CN103288974A - Method for extracting astragalus polysaccharide - Google Patents
Method for extracting astragalus polysaccharide Download PDFInfo
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- CN103288974A CN103288974A CN2013101730632A CN201310173063A CN103288974A CN 103288974 A CN103288974 A CN 103288974A CN 2013101730632 A CN2013101730632 A CN 2013101730632A CN 201310173063 A CN201310173063 A CN 201310173063A CN 103288974 A CN103288974 A CN 103288974A
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Abstract
The invention discloses a method for extracting astragalus polysaccharide. The method comprises the steps of: cutting astragalus membranaceus into 3-5cm of segments, adding water, refluxing for 2-3 hours to extract at 70-80 DEG C; separating extracting solution, and reserving filter residue; adding ethanol to the filter residue, stewing for 0.5-1 hour, adding 80-90 DEG C of water, refluxing for 0.4-0.6 hours, adding an enzyme preparation, controlling the temperature at 40-50 DEG C, adjusting the pH to 7-8; filtering to obtain filtrate after carrying out enzymolysis for 3-6 hours; merging the extracting solution with the filtrate; adding 95% of ethanol to carry out alcohol extraction, heating and warming to 95-100 DEG C, filtering to obtain solid when sediment appears after 20-30 minutes, and drying to obtain the astragalus polysaccharide. The ethanol is added to the filter residue; polysaccharide is further extracted; the enzyme preparation is added after water is added; the filtrate is obtained after enzymolysis; the next step of alcohol extraction is conveniently carried out; the extraction efficiency of the polysaccharide is improved.
Description
Technical field
The present invention relates to a kind of extracting method of vegetable polysaccharides, relate in particular to a kind of extraction of Astragalus Polysaccharides in Astragalus method.
Background technology
The Radix Astragali has tonifying Qi, diuresis, and the effect of hidroschesis improves effects such as body immunity, and astragalus polysaccharides is one of the most effective composition in the Radix Astragali.Extract polysaccharide from the Radix Astragali, mainly contain water extraction and microwave method, the water extraction extraction efficiency is low, and the step complexity, length consuming time, and loss of effective components is many; Microwave method is higher to equipment requirements, implements difficulty.Therefore, need provide a kind of new technical scheme to solve the problems referred to above.
Summary of the invention
The purpose of this invention is to provide the extraction of Astragalus Polysaccharides in Astragalus method that a kind of technology is simple, extraction efficiency is high.
The technical solution used in the present invention:
A kind of extraction of Astragalus Polysaccharides in Astragalus method is characterized in that, may further comprise the steps:
A, the Radix Astragali is cut into 3-5 centimetre segment, adds water, under 70-80 ℃, extracted in backflow 2-3 hour, separation and Extraction liquid, filter residue keeps;
Add ethanol in b, the filter residue, left standstill 0.5-1 hour, add 80-90 ℃ water, backflow 0.4-0.6 hour, add zymin again, 40-50 ℃ of control temperature, adjusting PH is 7-8, after enzymolysis 3-6 hour, filters, and gets filtrate;
C, united extraction liquid and filtrate, the ethanol that adds volumetric concentration 95% carries out alcohol extracting, heat temperature raising after 20-30 minute, occurs precipitation to 95-100 ℃, filter solid, after the drying astragalus polysaccharides.
Described zymin is neutral cellulase or neutral protease, and the add-on of zymin is the 2-3% of Radix Astragali percentage composition.
The mass ratio of the Radix Astragali and water is 1:3-5 in a step.
Add ethanol in the filter residue of b step, the mass ratio of ethanol and filter residue is 2-3:1.
Advantage of the present invention is: add ethanol in the filter residue, further extract polysaccharide, after adding water, add zymin again, behind the enzymolysis, get filtrate, be convenient to carry out next step alcohol extracting, improve the extraction yield of polysaccharide.
Embodiment
Following embodiment only is used for explanation the present invention, but can not limit protection scope of the present invention.
The technical solution used in the present invention:
Embodiment 1
A kind of extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, the Radix Astragali is cut into 3 centimetres segment, adds water, the mass ratio of the Radix Astragali and water is 1:3, under 70 ℃, and the extraction in 2 hours that refluxes, separation and Extraction liquid, filter residue keeps;
Add ethanol in b, the filter residue, add ethanol in the filter residue, the mass ratio of ethanol and filter residue is 2:1, left standstill 0.5 hour, and added 80 ℃ water, refluxed 0.4 hour, add zymin again, zymin is neutral cellulase, and the add-on of zymin is 2% of Radix Astragali percentage composition.40 ℃ of control temperature, regulating PH is 7, enzymolysis filtered after 3 hours, got filtrate;
C, united extraction liquid and filtrate, the ethanol that adds volumetric concentration 95% carries out alcohol extracting, heat temperature raising to 95 ℃ after 20 minutes, precipitation occurs, filter solid, after the drying astragalus polysaccharides.
Embodiment 2
A kind of extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, the Radix Astragali is cut into 4 centimetres segment, adds water, the mass ratio of the Radix Astragali and water is 1:4, under 75 ℃, and the extraction in 2.5 hours that refluxes, separation and Extraction liquid, filter residue keeps;
Add ethanol in b, the filter residue, add ethanol in the filter residue, the mass ratio of ethanol and filter residue is 2.5:1, left standstill 0.7 hour, and added 85 ℃ water, refluxed 0.5 hour, add zymin again, zymin is neutral protease, and the add-on of zymin is 2.5% of Radix Astragali percentage composition.45 ℃ of control temperature, regulating PH is 7.5, enzymolysis filtered after 4 hours, got filtrate;
C, united extraction liquid and filtrate, the ethanol that adds volumetric concentration 95% carries out alcohol extracting, heat temperature raising to 97 ℃ after 25 minutes, precipitation occurs, filter solid, after the drying astragalus polysaccharides.
Embodiment 3
A kind of extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, the Radix Astragali is cut into 5 centimetres segment, adds water, the mass ratio of the Radix Astragali and water is 1:5, under 80 ℃, and the extraction in 3 hours that refluxes, separation and Extraction liquid, filter residue keeps;
Add ethanol in b, the filter residue, add ethanol in the filter residue, the mass ratio of ethanol and filter residue is 3:1, left standstill 1 hour, and added 90 ℃ water, refluxed 0.6 hour, add zymin again, zymin is neutral cellulase, and the add-on of zymin is 3% of Radix Astragali percentage composition.50 ℃ of control temperature, regulating PH is 8, enzymolysis filtered after 6 hours, got filtrate;
C, united extraction liquid and filtrate, the ethanol that adds volumetric concentration 95% carries out alcohol extracting, heat temperature raising to 100 ℃ after 30 minutes, precipitation occurs, filter solid, after the drying astragalus polysaccharides.
Add ethanol in the filter residue, further extract polysaccharide, after adding water, add zymin again, behind the enzymolysis, get filtrate, be convenient to carry out next step alcohol extracting, improve the extraction yield of polysaccharide.
Enzyme handle to extract polysaccharide, have mild condition, easily remove impurity, keep the advantage of astragalus polysaccharides activity, the protein that enzyme can decompose in the Radix Astragali is peptide or amino acid, can accelerate the disintegration of organization structure of the plant, makes the bigger effectiveness of enzyme performance.
Claims (4)
1. an extraction of Astragalus Polysaccharides in Astragalus method is characterized in that, may further comprise the steps:
A, the Radix Astragali is cut into 3-5 centimetre segment, adds water, under 70-80 ℃, extracted in backflow 2-3 hour, separation and Extraction liquid, filter residue keeps;
Add ethanol in b, the filter residue, left standstill 0.5-1 hour, add 80-90 ℃ water, backflow 0.4-0.6 hour, add zymin again, 40-50 ℃ of control temperature, adjusting PH is 7-8, after enzymolysis 3-6 hour, filters, and gets filtrate;
C, united extraction liquid and filtrate, the ethanol that adds volumetric concentration 95% carries out alcohol extracting, heat temperature raising after 20-30 minute, occurs precipitation to 95-100 ℃, filter solid, after the drying astragalus polysaccharides.
2. a kind of extraction of Astragalus Polysaccharides in Astragalus method according to claim 1, it is characterized in that: described zymin is neutral cellulase or neutral protease, the add-on of zymin is the 2-3% of Radix Astragali percentage composition.
3. a kind of extraction of Astragalus Polysaccharides in Astragalus method according to claim 1, it is characterized in that: the mass ratio of the Radix Astragali and water is 1:3-5 in a step.
4. a kind of extraction of Astragalus Polysaccharides in Astragalus method according to claim 1, it is characterized in that: add ethanol in the filter residue of b step, the mass ratio of ethanol and filter residue is 2-3:1.
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| CN2013101730632A CN103288974A (en) | 2013-05-13 | 2013-05-13 | Method for extracting astragalus polysaccharide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104211835A (en) * | 2014-09-22 | 2014-12-17 | 山西大学 | Method for extracting araboxylan from astragalus medicine residues by using ionic liquid |
| CN104784248A (en) * | 2015-04-21 | 2015-07-22 | 四川国康药业有限公司 | Astragalus membranaceus extract, astragalus membranaceus tablet and preparation method thereof |
| CN104926951A (en) * | 2015-06-04 | 2015-09-23 | 季华 | Method for extracting astraglus polysaccharide |
| CN105412209A (en) * | 2015-12-08 | 2016-03-23 | 广东艾希德药业有限公司 | Astragalus extract and preparation method thereof and application |
| CN105777921A (en) * | 2014-12-26 | 2016-07-20 | 王贵孝 | Astragalus polysaccharide extraction process and application thereof |
| CN106977615A (en) * | 2017-03-10 | 2017-07-25 | 海门市协和农产品有限公司 | A kind of extracting method of efficient astragalus polyose |
| CN117122002A (en) * | 2023-09-11 | 2023-11-28 | 辅农(廊坊)生物科技有限公司 | Solid beverage for improving intestinal flora and preparation method thereof |
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| US20020131978A1 (en) * | 2001-03-19 | 2002-09-19 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
| CN101619107A (en) * | 2009-08-17 | 2010-01-06 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
| CN102875688A (en) * | 2012-10-05 | 2013-01-16 | 南通四海植物精华有限公司 | Process for extracting polysaccharide from milkvetch roots |
| CN102887963A (en) * | 2012-10-30 | 2013-01-23 | 河南牧翔动物药业有限公司 | Extraction method of astragalus polysaccharide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020131978A1 (en) * | 2001-03-19 | 2002-09-19 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
| CN101619107A (en) * | 2009-08-17 | 2010-01-06 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
| CN102875688A (en) * | 2012-10-05 | 2013-01-16 | 南通四海植物精华有限公司 | Process for extracting polysaccharide from milkvetch roots |
| CN102887963A (en) * | 2012-10-30 | 2013-01-23 | 河南牧翔动物药业有限公司 | Extraction method of astragalus polysaccharide |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104211835A (en) * | 2014-09-22 | 2014-12-17 | 山西大学 | Method for extracting araboxylan from astragalus medicine residues by using ionic liquid |
| CN105777921A (en) * | 2014-12-26 | 2016-07-20 | 王贵孝 | Astragalus polysaccharide extraction process and application thereof |
| CN104784248A (en) * | 2015-04-21 | 2015-07-22 | 四川国康药业有限公司 | Astragalus membranaceus extract, astragalus membranaceus tablet and preparation method thereof |
| CN104784248B (en) * | 2015-04-21 | 2019-01-18 | 四川国康药业有限公司 | A kind of Astragalus Root P.E, astragalus mongholicus tablet and preparation method thereof |
| CN104926951A (en) * | 2015-06-04 | 2015-09-23 | 季华 | Method for extracting astraglus polysaccharide |
| CN105412209A (en) * | 2015-12-08 | 2016-03-23 | 广东艾希德药业有限公司 | Astragalus extract and preparation method thereof and application |
| CN105412209B (en) * | 2015-12-08 | 2020-10-02 | 广东艾希德药业有限公司 | Astragalus extract and preparation method and application thereof |
| CN106977615A (en) * | 2017-03-10 | 2017-07-25 | 海门市协和农产品有限公司 | A kind of extracting method of efficient astragalus polyose |
| CN117122002A (en) * | 2023-09-11 | 2023-11-28 | 辅农(廊坊)生物科技有限公司 | Solid beverage for improving intestinal flora and preparation method thereof |
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Application publication date: 20130911 |