CN102875688A - Process for extracting polysaccharide from milkvetch roots - Google Patents
Process for extracting polysaccharide from milkvetch roots Download PDFInfo
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- CN102875688A CN102875688A CN2012103739213A CN201210373921A CN102875688A CN 102875688 A CN102875688 A CN 102875688A CN 2012103739213 A CN2012103739213 A CN 2012103739213A CN 201210373921 A CN201210373921 A CN 201210373921A CN 102875688 A CN102875688 A CN 102875688A
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- radix astragali
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Abstract
The invention discloses a process for extracting polysaccharide from milkvetch roots. The process is characterized by including the following steps of step a, using a dry milkvetch root powder as a raw material, placing the dry milkvetch root powder in a reaction vessel, and adding a mixed solvent to the reaction vessel; step b, heating a solution obtained after the mixing reaction in the step a, and adding a multienzyme complex to the mixed solution, wherein the multienzyme complex comprises cellulose and pectinase with the weight ratio of 1:1; step c, cooling the mixed solution obtained after enzymolysis reaction, and removing lower layer precipitates after the cooling to obtain supernatant; step d, concentrating the supernatant obtained from the step c to be paste, adding ethanol for precipitation, and performing filtering to obtain a milkvetch root polysaccharide crude product; and step e, refining the milkvetch root polysaccharide crude product to obtain a milkvetch root polysaccharide refined product. The process for extracting the polysaccharide from the milkvetch roots has the advantages of being simple in operation, conditions are easy to achieve, the yield rate of the polysaccharide is high, and the purity is high.
Description
Technical field
The present invention relates to a kind of technique of from the Radix Astragali, extracting polysaccharide, belong to medical technical field.
Background technology
Mainly contain polysaccharide, saponin(e, flavones, protein, Mierocrystalline cellulose and trace element in the Radix Astragali.Polysaccharide content is about about 20% in the natural Radix Astragali, the at present extraction of astragalus polysaccharides mainly contains water extraction and alcohol extracting method, but this extracting method exists problems: boil for a long time heating and can make astragalus polysaccharides be hydrolyzed destruction, cause the astragalus polysaccharides pharmacologically active to descend, and can other components in the Radix Astragali be extracted simultaneously, purity and the color of product all had a great impact.In addition, the extracting method workload of this mode is large, cost is high, yield and content are all lower.Therefore, should provide a kind of new technical scheme to address the above problem.
Summary of the invention
The objective of the invention is: for above-mentioned deficiency, provide the extraction process that a kind of astragalus polysaccharides productive rate is high, purity is high, cost is low.
For achieving the above object, the technical solution used in the present invention is:
A kind of technique of extracting polysaccharide from the Radix Astragali comprises the steps:
A, employing Radix Astragali dry powder are as starting material, and the particle size range of control Radix Astragali dry powder is the 40-60 order, and Radix Astragali dry powder is put into reaction vessel, then adds mixed solvent in reaction vessel, and the part by weight of control Radix Astragali dry powder and mixed solvent is 1:5-8;
B, the solution after the step a hybrid reaction is heated, the control Heating temperature is 80-90 ℃, then in mixing solutions, add multienzyme complex, enzymolysis 1-3h, described multienzyme complex comprises that part by weight is cellulase and the polygalacturonase of 1:1, and the part by weight of described multienzyme complex and Radix Astragali dry powder is 1:8-10;
C, the mixing solutions behind the enzyme digestion reaction is cooled off, the control temperature is 10-20 ℃, discards lower sediment after the cooling, obtains supernatant liquor;
D, supernatant concentration that step c is obtained add ethanol again and precipitate to paste shape, filter and obtain the astragalus polysaccharides crude product, and the part by weight of described ethanol and paste shape supernatant liquor is 4-6:1;
E, the astragalus polysaccharides crude product refining is namely got the astragalus polysaccharides highly finished product.
Mixed solvent among the described step a is the mixture of ethanol, water and propyl carbinol.
Part by weight=the 4:3:3 of described ethanol, water and propyl carbinol.
Advantage of the present invention is: the present invention extracts the extraction process of polysaccharide from the Radix Astragali, simple to operate, and condition is easy to realization, and the polysaccharide output capacity is high, and purity is high.Adopt multienzyme complex to carry out enzymolysis, improved greatly activity and the immunity of astragalus polysaccharides, keep Radix Astragali effective constituent and original color, smell and taste, improved to greatest extent the extraction yield of astragalus polysaccharides.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.Following examples just illustrate of the present invention, do not limit protection scope of the present invention.
Embodiment 1
A kind of technique of extracting polysaccharide from the Radix Astragali comprises the steps:
A, employing 50g Radix Astragali dry powder are as starting material, the particle diameter of control Radix Astragali dry powder is 40 orders, Radix Astragali dry powder is put into reaction vessel, then in reaction vessel, add the 250g mixed solvent, described mixed solvent is the mixture of ethanol, water and propyl carbinol, wherein part by weight=the 4:3:3 of ethanol, water and propyl carbinol;
B, with the solution after step a hybrid reaction heating, the control Heating temperature is 80 ℃, then adds the 6g multienzyme complex in mixing solutions, enzymolysis 1.5h, multienzyme complex comprise that part by weight is cellulase and the polygalacturonase of 1:1;
C, the mixing solutions behind the enzyme digestion reaction is cooled off, the control temperature is 10 ℃, discards lower sediment after the cooling, obtains the 150g supernatant liquor;
D, 150g supernatant concentration that step c is obtained add 600g ethanol again and precipitate to paste shape, filter and obtain the astragalus polysaccharides crude product;
E, the astragalus polysaccharides crude product refining is namely got 8g astragalus polysaccharides highly finished product.
Embodiment 2
A kind of technique of extracting polysaccharide from the Radix Astragali comprises the steps:
A, employing 100g Radix Astragali dry powder are as starting material, the particle diameter of control Radix Astragali dry powder is 50 orders, Radix Astragali dry powder is put into reaction vessel, then in reaction vessel, add the 500g mixed solvent, described mixed solvent is the mixture of ethanol, water and propyl carbinol, wherein part by weight=the 4:3:3 of ethanol, water and propyl carbinol;
B, with the solution after step a hybrid reaction heating, the control Heating temperature is 85 ℃, then adds the 12g multienzyme complex in mixing solutions, enzymolysis 2h, multienzyme complex comprise that part by weight is cellulase and the polygalacturonase of 1:1;
C, the mixing solutions behind the enzyme digestion reaction is cooled off, the control temperature is 15 ℃, discards lower sediment after the cooling, obtains the 300g supernatant liquor;
D, 300g supernatant concentration that step c is obtained add 1200g ethanol again and precipitate to paste shape, filter and obtain the astragalus polysaccharides crude product;
E, the astragalus polysaccharides crude product refining is namely got 16g astragalus polysaccharides highly finished product.
Embodiment 3
A kind of technique of extracting polysaccharide from the Radix Astragali comprises the steps:
A, employing 150g Radix Astragali dry powder are as starting material, the particle diameter of control Radix Astragali dry powder is 60 orders, Radix Astragali dry powder is put into reaction vessel, then in reaction vessel, add the 750g mixed solvent, described mixed solvent is the mixture of ethanol, water and propyl carbinol, wherein part by weight=the 4:3:3 of ethanol, water and propyl carbinol;
B, with the solution after step a hybrid reaction heating, the control Heating temperature is 85 ℃, then adds the 18g multienzyme complex in mixing solutions, enzymolysis 3h, multienzyme complex comprise that part by weight is cellulase and the polygalacturonase of 1:1;
C, the mixing solutions behind the enzyme digestion reaction is cooled off, the control temperature is 20 ℃, discards lower sediment after the cooling, obtains the 450g supernatant liquor;
D, 450g supernatant concentration that step c is obtained add 1800g ethanol again and precipitate to paste shape, filter and obtain the astragalus polysaccharides crude product;
E, the astragalus polysaccharides crude product refining is namely got 25g astragalus polysaccharides highly finished product.
Claims (3)
1. a technique of extracting polysaccharide from the Radix Astragali is characterized in that, comprises the steps:
A, employing Radix Astragali dry powder are as starting material, and the particle size range of control Radix Astragali dry powder is the 40-60 order, and Radix Astragali dry powder is put into reaction vessel, then adds mixed solvent in reaction vessel, and the part by weight of control Radix Astragali dry powder and mixed solvent is 1:5-8;
B, the solution after the step a hybrid reaction is heated, the control Heating temperature is 80-90 ℃, then in mixing solutions, add multienzyme complex, enzymolysis 1-3h, described multienzyme complex comprises that part by weight is cellulase and the polygalacturonase of 1:1, and the part by weight of described multienzyme complex and Radix Astragali dry powder is 1:8-10;
C, the mixing solutions behind the enzyme digestion reaction is cooled off, the control temperature is 10-20 ℃, discards lower sediment after the cooling, obtains supernatant liquor;
D, supernatant concentration that step c is obtained add ethanol again and precipitate to paste shape, filter and obtain the astragalus polysaccharides crude product, and the part by weight of described ethanol and paste shape supernatant liquor is 4-6:1;
E, the astragalus polysaccharides crude product refining is namely got the astragalus polysaccharides highly finished product.
2. a kind of technique of extracting polysaccharide from the Radix Astragali according to claim 1, it is characterized in that: the mixed solvent among the described step a is the mixture of ethanol, water and propyl carbinol.
3. a kind of technique of extracting polysaccharide from the Radix Astragali according to claim 2 is characterized in that: the part by weight=4:3:3 of described ethanol, water and propyl carbinol.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103739213A CN102875688A (en) | 2012-10-05 | 2012-10-05 | Process for extracting polysaccharide from milkvetch roots |
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| CN2012103739213A CN102875688A (en) | 2012-10-05 | 2012-10-05 | Process for extracting polysaccharide from milkvetch roots |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288974A (en) * | 2013-05-13 | 2013-09-11 | 茅兴娟 | Method for extracting astragalus polysaccharide |
| CN103382228A (en) * | 2013-07-31 | 2013-11-06 | 瑞普（天津）生物药业有限公司 | Preparation method for high-activity astragalus polysaccharide |
| CN103613674A (en) * | 2013-09-09 | 2014-03-05 | 华南农业大学 | Extraction method for astragalus polysacharin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| CN1709916A (en) * | 2005-06-15 | 2005-12-21 | 甘肃祁连山生物科技开发有限责任公司 | Method for extracting high purity polysaccharide of membranous milk vetch and saponin of same |
| CN101619107A (en) * | 2009-08-17 | 2010-01-06 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
-
2012
- 2012-10-05 CN CN2012103739213A patent/CN102875688A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| CN1709916A (en) * | 2005-06-15 | 2005-12-21 | 甘肃祁连山生物科技开发有限责任公司 | Method for extracting high purity polysaccharide of membranous milk vetch and saponin of same |
| CN101619107A (en) * | 2009-08-17 | 2010-01-06 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288974A (en) * | 2013-05-13 | 2013-09-11 | 茅兴娟 | Method for extracting astragalus polysaccharide |
| CN103382228A (en) * | 2013-07-31 | 2013-11-06 | 瑞普（天津）生物药业有限公司 | Preparation method for high-activity astragalus polysaccharide |
| CN103613674A (en) * | 2013-09-09 | 2014-03-05 | 华南农业大学 | Extraction method for astragalus polysacharin |
| CN103613674B (en) * | 2013-09-09 | 2015-12-02 | 华南农业大学 | A kind of extracting method of astragalus polysaccharides |
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Application publication date: 20130116 |