CN103283488A - Method for purifying substrate mycelium from edible fungi mother culture - Google Patents
Method for purifying substrate mycelium from edible fungi mother culture Download PDFInfo
- Publication number
- CN103283488A CN103283488A CN2013102428669A CN201310242866A CN103283488A CN 103283488 A CN103283488 A CN 103283488A CN 2013102428669 A CN2013102428669 A CN 2013102428669A CN 201310242866 A CN201310242866 A CN 201310242866A CN 103283488 A CN103283488 A CN 103283488A
- Authority
- CN
- China
- Prior art keywords
- substrate mycelium
- purification
- bacterial classification
- medium
- edible fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a method for purifying substrate mycelium. In an edible fungi mother culture totally polluted by bacteria, the substrate mycelium is purified after fully growing, and a culture which is high in purity, pure in color, and exuberant in growth, and keeps culture original characters can be obtained again. The method for purifying the substrate mycelium is an effective rescue measure to prevent rare cultures from loss.
Description
Technical field
Content of the present invention relates to a kind of substrate mycelium purification of parent edible fungus kind and the method for purifying, and belongs to the edible mashroom cultivating technical field.
Background technology
Edible mushroom is to do bacterial classification with mycelium, in bacterial classification manufacturing process, often runs into the polluted parent problem, and pollution source are mainly mould and bacterium, thereby causes the bacterial classification performance degradation.
Because the color of fungal hyphae is more special, be different from color--the white of hypha of edible fungus, so after it pollutes bacterial classification, be easy to be identified.And after the germ contamination bacterial classification, if rejected in the untimely inspection of when beginning and to female kind of problematic test tube, so after the meticulous bacterium bacterium colony of edible fungi wire cover, owing to can't identify in appearance, can cause such test tube female plant to escape from check and be applied to change expand and breed, like this, pollution range is enlarged, cause by the gross bacterial classification to scrap at last.
Germ contamination medium sterilization does not often thoroughly cause.It is not thorough to sterilize, and just has bacterial spore to stay in the medium, and one pot of property consequence just causes thus.Even but be subjected to germ contamination, also have certain rescue method.This method is exactly to utilize bacterial clump and the hypha of edible fungus different manifestations on medium, and the substrate mycelium of edible mushroom is purified, and can effectively avoid fine provenance to lose like this.
Summary of the invention
The present invention has following two aspect contents:
1, (annotated: the prerequisite that obtains the substrate mycelium of edible mushroom the cultivation time of cultivating substrate mycelium and fully growing required, be that substrate mycelium must be fully flourishing, it reaches the flourishing required time and is longer than that test tube is female plants conventional growth time, how long the time period that exceeds so has on earth, is within the present invention and holds.)
2, the method for purification of substrate mycelium
The technical solution adopted in the present invention is:
The parent edible fungus kind of selecting only to have polluted bacterium continues to cultivate, and makes the cultivation substrate mycelium fully grow into the bottom.Total cultivation time of the class of generally picking up the ears bacterial classification is 20d-22d, and mushroom, auricularia auriculajudae, Asparagus class bacterial classification are 25d-27d, and hedgehog fungus bacterial is 32d.
The method of purification of substrate mycelium:
1. sterilization: the plate of the tweezers of slant tube that will be to be inoculated, alcolhol burner, match, inoculation shovel, large size, cotton ball soaked in alcohol, Sheng refuse etc. is placed on the superclean bench, opens uviol lamp, sterilization 15min～20min;
2. the mother of polluted bacteria is planted test tube with cotton ball soaked in alcohol wiping sterilization repeatedly, put into superclean bench, will inoculate shovel again, tweezers (especially holding) are sterilized at alcolhol burner flame;
3. after treating the tweezers cooling, with tweezers end is smashed bacterial classification pipe bottom (contact medium one side), the medium back side is exposed;
4. the careful medium (preferably get the medium of mid portion, this part medium substrate mycelium is flourishing) of getting the soya bean size that shovels is seeded on the slant tube from the back side with the inoculation shovel;
5. will inoculate good bacterial classification puts into culturing room and cultivates, temperature control is at 20 ℃～22 ℃, humidity control is 60%～75%, check after cultivating 1d～2d, find not growth or the timely rejecting that pollution is arranged, stay purity height, color and luster just, robust growth and keep the bacterial classification of the original proterties of kind.
Content of the present invention is: taking place in the parent edible fungus kind of germ contamination comprehensively, by a kind of substrate mycelium method of purification, obtain again purity height, color and luster just, robust growth and keep the bacterial classification of the original proterties of kind.This method is one to prevent the precious former effective remedial measure lost of planting.
Embodiment
Mode 1: the substrate mycelium of flat mushroom strain is purified in the present embodiment, continues to cultivate for the flat mushroom test tube strains of only having polluted bacterium, and total cultivation time is 20d, makes flat mushroom cultivate substrate mycelium and fully grows into the bottom.
Method of purification:
1. sterilization: the plate of the tweezers of slant tube that will be to be inoculated, alcolhol burner, match, inoculation shovel, large size, cotton ball soaked in alcohol, Sheng refuse etc. is placed on the superclean bench, opens uviol lamp, sterilization 15min～20min;
2. the mother of polluted bacteria is planted test tube with cotton ball soaked in alcohol wiping sterilization repeatedly, put into superclean bench, will inoculate shovel again, tweezers (especially holding) are sterilized at alcolhol burner flame;
3. after treating the tweezers cooling, with tweezers end is smashed bacterial classification pipe bottom (contact medium one side), the medium back side is exposed;
4. the careful medium (preferably get the medium of mid portion, this part medium substrate mycelium is flourishing) of getting the soya bean size that shovels is seeded on the slant tube from the back side with the inoculation shovel;
5. will inoculate good bacterial classification and put into culturing room and cultivate, temperature control at 22 ℃, and humidity control 65%, checks discovery is not grown or the timely rejecting that pollution is arranged after cultivating 1d.
The female cultivation of planting through 8d of flat mushroom test tube after the purification, hypha of Pleurotus ostreatus dense white, close, aerial hyphae is more, colony edge is neat, prosperous, the fast growth of mycelia growing way, has the distinctive proterties of former kind.
Mode 2: the substrate mycelium of woodear bacterial classification is purified in the present embodiment, continues to cultivate for the woodear test tube strains of only having polluted bacterium, and total cultivation time is 27d, makes the interior mycelia of blackfungus culture medium fully grow into the bottom.Method of purification reference example 1.
The female cultivation of planting through 10d of woodear test tube after the purification, black fungus bacterial filament length gesture is prosperous, density is big, and mycelia is pure white, colony edge is neat and show stronger vitality, has the distinctive proterties of former kind.
Mode 3: the substrate mycelium of hedgehog fungus bacterial is purified in the present embodiment, continues to cultivate for the Hericium erinaceus test tube strains of only having polluted bacterium, and total cultivation time is 32d, makes Hericium erinaceus cultivate substrate mycelium and fully grows into the bottom.Method of purification reference example 1.
The female cultivation of planting through 15d of Hericium erinaceus test tube after the purification, the hericium mycelium canescence, dense, be close to slant medium, short and sparse, substrate mycelium is many, has the distinctive proterties of former kind.
Claims (5)
1. the substrate mycelium method of purification of a parent edible fungus kind is characterized in that the cultivation time that definite various parent edible fungus kind substrate myceliums fully grow required, and the method for operating of substrate mycelium purification.
2. the substrate mycelium method of purification of parent edible fungus kind according to claim 1, it is characterized in that making cultivation time that class bacterial classification substrate mycelium fully grows required of picking up the ears is 20d-22d.
3. the substrate mycelium method of purification of parent edible fungus kind according to claim 1 is characterized in that the cultivation time that makes mushroom, auricularia auriculajudae, Asparagus class bacterial classification substrate mycelium fully grow required is 25d-27d.
4. the substrate mycelium method of purification of parent edible fungus kind according to claim 1 is characterized in that the cultivation time that makes the hedgehog fungus bacterial substrate mycelium fully grow required is 32d.
5. the substrate mycelium method of purification of the parent edible fungus kind described in the claim 1-4 comprises the steps:
1. sterilization: the plate of the tweezers of slant tube that will be to be inoculated, alcolhol burner, match, inoculation shovel, large size, cotton ball soaked in alcohol, Sheng refuse etc. is placed on the superclean bench, opens uviol lamp, sterilization 15min～20min;
2. the mother of polluted bacteria is planted test tube with cotton ball soaked in alcohol wiping sterilization repeatedly, put into superclean bench, will inoculate shovel again, tweezers (especially holding) are sterilized at alcolhol burner flame;
3. after treating the tweezers cooling, with tweezers end is smashed bacterial classification pipe bottom (contact medium one side), the medium back side is exposed;
4. the careful medium (preferably get the medium of mid portion, this part medium substrate mycelium is flourishing) of getting the soya bean size that shovels is seeded on the slant tube from the back side with the inoculation shovel;
5. will inoculate good bacterial classification puts into culturing room and cultivates, temperature control is at 20 ℃～22 ℃, humidity control is 60%～75%, check after cultivating 1d～2d, find not growth or the timely rejecting that pollution is arranged, stay purity height, color and luster just, robust growth and keep the bacterial classification of the original proterties of kind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102428669A CN103283488A (en) | 2013-06-06 | 2013-06-06 | Method for purifying substrate mycelium from edible fungi mother culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102428669A CN103283488A (en) | 2013-06-06 | 2013-06-06 | Method for purifying substrate mycelium from edible fungi mother culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103283488A true CN103283488A (en) | 2013-09-11 |
Family
ID=49085475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102428669A Pending CN103283488A (en) | 2013-06-06 | 2013-06-06 | Method for purifying substrate mycelium from edible fungi mother culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103283488A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488557A (en) * | 2014-12-26 | 2015-04-08 | 青岛华盛绿能农业科技有限公司 | Method for purification and rejuvenation of edible fungus strain |
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
| CN111424104A (en) * | 2020-01-21 | 2020-07-17 | 福建农林大学 | Method for rapidly judging degradation of pleurotus eryngii strain based on bacterial community composition and abundance and application of method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084345A (en) * | 1993-08-21 | 1994-03-30 | 史新洋 | Method for purification of hypha of edible fungus |
-
2013
- 2013-06-06 CN CN2013102428669A patent/CN103283488A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084345A (en) * | 1993-08-21 | 1994-03-30 | 史新洋 | Method for purification of hypha of edible fungus |
Non-Patent Citations (1)
| Title |
|---|
| 高晓华: "一级种的基内菌丝提纯法", 《食用菌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488557A (en) * | 2014-12-26 | 2015-04-08 | 青岛华盛绿能农业科技有限公司 | Method for purification and rejuvenation of edible fungus strain |
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
| CN111424104A (en) * | 2020-01-21 | 2020-07-17 | 福建农林大学 | Method for rapidly judging degradation of pleurotus eryngii strain based on bacterial community composition and abundance and application of method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101653081B (en) | Artificial culture method of xylaria gracillima | |
| CN102633552A (en) | Formula of culture medium for white beech mushroom and method for cultivating white beech mushroom | |
| CN103430776A (en) | Silkworm pupa-worm grass culture method capable of improving yield and quality | |
| CN102440147A (en) | Production technology of edible mushroom liquid spawn by adopting reducing process | |
| CN106105773A (en) | The producing method for seed of edible mushroom cultivated species and prepared cultigen and cultivating method for edible fungi | |
| CN111763627B (en) | Fungus for promoting growth of medicinal dendrobium officinale seedlings | |
| CN103688759A (en) | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers | |
| CN101565689A (en) | Production method for high-density pure arbuscular mycorrhizal fungal spore | |
| CN101263778A (en) | Method for fast establishing DSE and plant symbiosis cultivation system and uses thereof | |
| CN103283488A (en) | Method for purifying substrate mycelium from edible fungi mother culture | |
| CN101138317B (en) | Tissue inner carrying bacterium explant disinfection processing method | |
| CN105132294B (en) | A kind of alternaric bacteria and its application in dendrobium candidum disease-resistanting leaf spot | |
| CN101182471B (en) | Highly effective cultivating method for Chinese caterpillar fungus | |
| CN103270890B (en) | A kind of Antrodia camphorata ware formula cultural method | |
| CN105754872A (en) | Method for fast separating, identifying and storing sweet potato sclerotium rolfsii | |
| CN103704022A (en) | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier | |
| CN102523927A (en) | Method for culturing cordyceps militaris in natural environment | |
| CN103688760A (en) | Method for culturing artificial cordyceps sinensis by using yellow mealworms as carriers | |
| CN102286385A (en) | Method for carrying out solid culturing on beauveria bassiana by stages | |
| CN102396343A (en) | Method for fruiting and breeding needle mushrooms on flat plate through multi-spore self-breeding | |
| CN104126508B (en) | The method of the quick Mycorrhizal of a kind of orchid aseptic seedling | |
| US20150250103A1 (en) | Mycelial Mass with Non-electrical Carbon Dioxide Transfer | |
| CN103688744B (en) | A kind of Cordceps militaris breeding method and using method | |
| CN103749149A (en) | Method for cultivating artificial worm grass with mealworm pupa as carrier | |
| JP4691307B2 (en) | Microbial growth control method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130911 |