CN109220560A - A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification - Google Patents
A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification Download PDFInfo
- Publication number
- CN109220560A CN109220560A CN201811391102.5A CN201811391102A CN109220560A CN 109220560 A CN109220560 A CN 109220560A CN 201811391102 A CN201811391102 A CN 201811391102A CN 109220560 A CN109220560 A CN 109220560A
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- Prior art keywords
- base
- purification
- critical point
- strain
- test tube
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to field of biotechnology, specifically a kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification.The steps include: for separated fruit body of edible fungi meat piece tissue to be placed in blank cuvette from slant medium have 2~5 millimeters of upper sides without base and have base critical point, mycelia sprouted under no media environment before this, after mycelia grows to contact culture medium, mycelia growth is rapid, vigorous, the mycelia of this state is passed through into purification, can effective detoxification, keep the stable excellent moral character of bacterial strain.Strain manufacturing process simple process of the invention, strong applicability, the tissue suitable for most edible mushroom strains separate breeding, and success rate can reach 99%.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to the test tube to a kind of Needle mushroom strain without base and has base critical point
Breeding, method of purification.
Background technique
China's Edible Fungi causes some edible fungus on the market there are still the tendency of " weight yield, lightweight "
It lacks competitiveness.Meanwhile the scientific research strength in terms of strain breeding is more weak, making strain successive propagation too much causes strain living
Power is degenerated, and the superiority and inferiority of strain directly influences the yield and quality of edible fungus.Focus on high-quality, high yield, disease-resistant, pest-resistant etc.
The breeding of strain excellent is to guarantee that edible mushroom improves the only way of product yield, quality, and improve Edible Fungi level
Important link.
Any one good edible fungus variety, if continuous cultivation or long-term preservation and turn generation can all have life in the process
Produce the phenomenon that performance deteriorates, kind property fails.So edible fungus species are separated and are screened, detoxification purification and rejuvenation, it can be effective
The degeneration for preventing kind and the most important thing during variation and edible fungus culturing.Therefore it periodically to introduce a fine variety or purificate and rejuvenate,
It just can guarantee that strain quality and kind property are stablized, to lay the foundation for ultimate output stabilization.Currently, each Edible Fungi person in order to
Guarantee that kind of a property is stablized, mostly use organize separation, stringent screening regularly greatly, acquisition strain, technique in this way carries out tissue
Separation, purification, it is necessary to be just able to achieve by tube more than three times and guarantee that strain quality and kind property are stablized.
" a kind of tube edible-fungus strain producing and transplanting method " that number of patent application is 98126599.5, operation of the present invention side
Just, quickly, time saving, laborsaving, switchable at any time, no less than 100 test tubes of switching in average everyone 1 hour, 99% or more survival rate,
Pollution rate 0.5% hereinafter, but the technique cannot effectively carry out detoxification, strain quality is unstable.
Number of patent application be 200910259431.9 " using protoplast regeneration technology carry out edible fungus species rejuvenation with
The method of detoxification ", the present invention and achieve the purpose that make edible mushroom rejuvenation and detoxification, restore the good characteristic of edible mushroom.But the work
Skill is sufficiently complex, and common people do not grasp.
Summary of the invention
The purpose of the present invention is to provide a kind of test tubes of Needle mushroom strain without base and to have base critical point breeding, purification side
The disadvantages of method, the strain quality that it overcomes conventional art process production is unstable, technique is sufficiently complex.It provides a kind of edible
The test tube of bacterium strain is without base and has base critical point breeding, method of purification technique.
The present invention is achieved through the following technical solutions:
The test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification, and processing step is as follows:
1, tissue separation: the fruit body of edible fungi of the excellent stalwartness of selection, in an aseptic environment by fructification cap and stem
Intersection cuts the meat piece tissue of 0.5~1 centimeter length.
2, test tube cultivation: there are 2~5 millimeters of sides from slant medium for what meat piece tissue was placed in blank PDA slant tube
Top without base and having base critical point.
3, cultural hypha: the test tube for connecting kind is placed under 20~25 DEG C of temperature, 75~80% environment of relative humidity and is trained
Bacteria silk.
4, tube purifies: after mycelia grows to contact slant medium, picking grows swift and violent, vigorous in an aseptic environment
Mycelia is forwarded to blank PDA slant tube, carries out strain purification.
Compared with prior art, the invention has the following advantages that
(1) present invention process is simple, effect is obvious.
(2) innovative point major embodiment of the invention was sprouted under no media environment before this in mycelia, when mycelia grows to
Contact culture medium after, mycelia growth it is rapid, vigorous, by the mycelia of this state by purification, can effective detoxification, keep bacterial strain it is steady
Fixed excellent moral character.
(3) the strain success rate that the present invention cultivates can reach 99%.
(4) the bright tissue suitable for most Strains of Flammulina velutipes of we separates, and can effectively purify, detoxification.
Specific embodiment
Method of the invention is further illustrated below with reference to embodiment.
The test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification, and processing step is as follows:
1, tissue separation: the fruit body of edible fungi of the excellent stalwartness of selection, in an aseptic environment by fructification cap and stem
Intersection cuts the meat piece tissue of 0.5~1 centimeter length.
2, test tube cultivation: there are 2~5 millimeters of sides from slant medium for what meat piece tissue was placed in blank PDA slant tube
Top without base and having base critical point.
3, cultural hypha: the test tube for connecting kind is placed under 20~25 DEG C of temperature, 75~80% environment of relative humidity and is trained
Bacteria silk.
4, tube purifies: after mycelia grows to contact slant medium, picking grows swift and violent, vigorous in an aseptic environment
Mycelia is forwarded to blank PDA slant tube, carries out strain purification.
Claims (1)
1. a kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification, it is characterized in that processing step is as follows:
(1) tissue separates: the fruit body of edible fungi of the excellent stalwartness of selection in an aseptic environment has a common boundary fructification cap and stem
Cut the meat piece tissue of 0.5~1 centimeter length in place.
(2) test tube cultivation: there are 2~5 millimeters of upper sides from slant medium for what meat piece tissue was placed in blank PDA slant tube
Without base and having base critical point.
(3) cultural hypha: the test tube for connecting kind is placed under 20~25 DEG C of temperature, 75~80% environment of relative humidity and cultivates bacterium
Silk.
(4) tube purifies: after mycelia grows to contact slant medium, picking grows swift and violent, vigorous bacterium in an aseptic environment
Silk is forwarded to blank PDA slant tube, carries out strain purification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391102.5A CN109220560A (en) | 2018-11-19 | 2018-11-19 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811391102.5A CN109220560A (en) | 2018-11-19 | 2018-11-19 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109220560A true CN109220560A (en) | 2019-01-18 |
Family
ID=65076129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811391102.5A Pending CN109220560A (en) | 2018-11-19 | 2018-11-19 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
Country Status (1)
| Country | Link |
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| CN (1) | CN109220560A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113455289A (en) * | 2021-08-05 | 2021-10-01 | 河南省农业科学院植物营养与资源环境研究所 | Method for rapidly detecting fruiting performance of edible mushroom tissue isolated strain |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084345A (en) * | 1993-08-21 | 1994-03-30 | 史新洋 | Method for purification of hypha of edible fungus |
| CN1796536A (en) * | 2004-12-22 | 2006-07-05 | 任玉凤 | Purifying polluted parent edible fungus |
| CN101773050A (en) * | 2009-12-22 | 2010-07-14 | 唐山师范学院 | Method for rejuvenating and detoxifying edible fungal strain by using protoplast regeneration technology |
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN103250548A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Edible fungus strain taking method besides tissue isolation |
| CN103283488A (en) * | 2013-06-06 | 2013-09-11 | 邬金飞 | Method for purifying substrate mycelium from edible fungi mother culture |
-
2018
- 2018-11-19 CN CN201811391102.5A patent/CN109220560A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084345A (en) * | 1993-08-21 | 1994-03-30 | 史新洋 | Method for purification of hypha of edible fungus |
| CN1796536A (en) * | 2004-12-22 | 2006-07-05 | 任玉凤 | Purifying polluted parent edible fungus |
| CN101773050A (en) * | 2009-12-22 | 2010-07-14 | 唐山师范学院 | Method for rejuvenating and detoxifying edible fungal strain by using protoplast regeneration technology |
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN103250548A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Edible fungus strain taking method besides tissue isolation |
| CN103283488A (en) * | 2013-06-06 | 2013-09-11 | 邬金飞 | Method for purifying substrate mycelium from edible fungi mother culture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113455289A (en) * | 2021-08-05 | 2021-10-01 | 河南省农业科学院植物营养与资源环境研究所 | Method for rapidly detecting fruiting performance of edible mushroom tissue isolated strain |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190118 |
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| WD01 | Invention patent application deemed withdrawn after publication |