CN103250548A - Edible fungus strain taking method besides tissue isolation - Google Patents
Edible fungus strain taking method besides tissue isolation Download PDFInfo
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- CN103250548A CN103250548A CN2012100343553A CN201210034355A CN103250548A CN 103250548 A CN103250548 A CN 103250548A CN 2012100343553 A CN2012100343553 A CN 2012100343553A CN 201210034355 A CN201210034355 A CN 201210034355A CN 103250548 A CN103250548 A CN 103250548A
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- edible fungus
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- mycelia
- edible
- tissue isolation
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Abstract
The invention relates to the technical field of biology, in particular to an edible fungus strain taking method besides tissue isolation. The method particularly includes the steps: performing tissue isolation by the aid of growing primordia in edible fungus test tube mother strain or bottled (bagged) stock culture; and transferring strains to other tubes and purifying the strains. The method is novel and simple in process and applicable to tissue isolation of most edible fungus strains. Hyphae cultured by the method are robust, strong white, uniform, high in growth speed and obviously superior to test tube strains cultured by traditional tissue isolation.
Description
Technical field
The present invention relates to biological technical field, be specifically related to a kind of edible fungus species tissue and get kind of a method beyond separating.
Background technology
China's Edible Fungi is generally comparatively weak in the scientific research strength of bacterial classification aspect breeding, most of Edible Fungi persons purchase the bacterial classification that uses others in the middle of production and operation, or produce with bacterial classification tube use repeatedly, ignore the bacterial classification successive propagation and caused spawn activity to be degenerated too much.The quality of bacterial classification directly has influence on output and the quality of edible fungus.Paying attention to breeding of high-quality, high yield, strain excellent such as disease-resistant, pest-resistant, is to guarantee that edible mushroom improves the only way of product yield, quality, also is the important step that improves the Edible Fungi level.
If the edible fungus variety that any one is good is cultivation or long-term preservation and change for all existing productivity to become phenomenon bad, the decline of kind property in the process continuously.So edible fungus species is organized separation, screening and detoxification purification and rejuvenation, can effectively prevent degeneration and the variation of kind, also be the most important thing in the edible fungus culturing process.Therefore the tissue of GPRS edible fungus species separates or the purification and rejuvenation technology, could guarantee effectively that strain quality and kind property are stable, thereby lay the first stone for final stable yield.
Current, the bacterial classification tissue that each Edible Fungi person adopts separates, be the fruit body of edible fungi of selecting good stalwartness mostly, and get on the medium that cube meat tissue in the fruit body is positioned over blank PDA slant tube and cultivate mycelia, through behind twice above tube and strict screening and obtain bacterial classification, technology is very traditional and extremely loaded down with trivial details again.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art and provide brand-new a kind of edible fungus species tissue to get kind of a method beyond separating, specifically comprise: the original hase that grows when taking the female kind of edible mushroom test tube or bottle (bag) dress original seed to cultivate is organized separation, and tube is purified.
The present invention is achieved through the following technical solutions:
1, a kind of edible fungus species tissue separates kind of the method for getting in addition, comprises in its technical scheme: the original hase that grows when taking the female kind of edible mushroom test tube or the cultivation of bottle (bag) dress original seed is organized separation, and tube is purified.
2, a kind of edible fungus species tissue separates kind of the method for getting in addition, and processing step is as follows in its technical scheme:
(1) with the long edible mushroom test tube that original hase arranged female plant or bottle (bag) dress original seed under gnotobasis with pocket knife with growing way by force, the original hase individuality that is about to be differentiated to form the mushroom flower bud extracts the fritter of mung bean grain size, move on the medium of receiving blank PDA slant tube with the inoculation hook then.
(2) after tissue separates, cultivate mycelia.
(3) account for when in vitro cultivating on the basal plane 1/3 when mycelia is about, swift and violent, the vigorous mycelia of picking growth is forwarded to blank PDA slant tube and carries out bacterial classification and purify under gnotobasis.
Compared with prior art, the present invention has the following advantages:
1, method technology novelty proposed by the invention, simple.
2, the present invention is in implementation process, and original hase can directly move pipe cultivation mycelia once extracting separation, and is convenient and swift; And traditional bright massee fruiting bodies separates, and also will be chosen in the skin and flesh of fruit body cap and stem intersection excision outside, only gets the cube meat tissue of the inside as bacterial classification, and operation is many and loaded down with trivial details.
3, the mycelia stalwartness of cultivating out through the present invention, dense white, neat and consistent, fast growth; Obviously be better than the test tube strains that the tradition tissue separates.
4, the bright tissue of most edible mushroom strains that is applicable to of we separates.
5, through the present invention cultivate the bacterial classification success rate can reach 99%.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
A kind of edible fungus species tissue separates kind of the method for getting in addition, and specific implementation method is as follows:
1, a kind of edible fungus species tissue separates kind of the method for getting in addition, comprises in its technical scheme: the original hase that grows when taking the female kind of edible mushroom test tube or the cultivation of bottle (bag) dress original seed is organized separation, and tube is purified.
2, a kind of edible fungus species tissue separates kind of the method for getting in addition, and processing step is as follows in its technical scheme:
(1) with the long edible mushroom test tube that original hase arranged female plant or bottle (bag) dress original seed under gnotobasis with pocket knife with growing way by force, the original hase individuality that is about to be differentiated to form the mushroom flower bud extracts the fritter of mung bean grain size, move on the medium of receiving blank PDA slant tube with the inoculation hook then.
(2) after tissue separates, cultivate mycelia.
(3) account for when in vitro cultivating on the basal plane 1/3 when mycelia is about, swift and violent, the vigorous mycelia of picking growth is forwarded to blank PDA slant tube and carries out bacterial classification and purify under gnotobasis.
Claims (2)
1. get kind of a method beyond an edible fungus species tissue separates, its feature comprises: take that the edible mushroom test tube is female plants or original hase that bottle (bag) dress original seed grows when cultivating is organized separation, the tube purification.
2. according to kind of the method for getting beyond a kind of edible fungus species tissue separation described in the claim 1, it is characterized in that processing step is as follows:
(1) with the long edible mushroom test tube that original hase arranged female plant or bottle (bag) dress original seed under gnotobasis with pocket knife with growing way by force, the original hase individuality that is about to be differentiated to form the mushroom flower bud extracts the fritter of mung bean grain size, move on the medium of receiving blank PDA slant tube with the inoculation hook then.
(2) after tissue separates, cultivate mycelia.
(3) account for when in vitro cultivating on the basal plane 1/3 when mycelia is about, swift and violent, the vigorous mycelia of picking growth is forwarded to blank PDA slant tube and carries out bacterial classification and purify under gnotobasis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100343553A CN103250548A (en) | 2012-02-15 | 2012-02-15 | Edible fungus strain taking method besides tissue isolation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100343553A CN103250548A (en) | 2012-02-15 | 2012-02-15 | Edible fungus strain taking method besides tissue isolation |
Publications (1)
| Publication Number | Publication Date |
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| CN103250548A true CN103250548A (en) | 2013-08-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2012100343553A Pending CN103250548A (en) | 2012-02-15 | 2012-02-15 | Edible fungus strain taking method besides tissue isolation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488557A (en) * | 2014-12-26 | 2015-04-08 | 青岛华盛绿能农业科技有限公司 | Method for purification and rejuvenation of edible fungus strain |
| CN105393792A (en) * | 2015-11-12 | 2016-03-16 | 黄福忠 | Method for taking strain and inoculation of strain from bottom of original strain bag |
| CN106386161A (en) * | 2015-08-11 | 2017-02-15 | 天津市庆祥农业科技有限公司 | Method for preparing edible fungus strains |
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279004A (en) * | 2000-08-01 | 2001-01-10 | 韩省华 | Equipment and technology for producing edible fungus spawn |
| JP2007053928A (en) * | 2005-08-23 | 2007-03-08 | Kinokkusu:Kk | New fungal strain and method for culturing new fruit body |
| CN101473758A (en) * | 2009-01-19 | 2009-07-08 | 北京林业大学 | Method for tissue separation of edible fungus |
| CN102172173A (en) * | 2011-03-04 | 2011-09-07 | 广东省微生物研究所 | Method for cultivating and producing sporocarps of dictyophora multicolor berk. and broome |
-
2012
- 2012-02-15 CN CN2012100343553A patent/CN103250548A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279004A (en) * | 2000-08-01 | 2001-01-10 | 韩省华 | Equipment and technology for producing edible fungus spawn |
| JP2007053928A (en) * | 2005-08-23 | 2007-03-08 | Kinokkusu:Kk | New fungal strain and method for culturing new fruit body |
| CN101473758A (en) * | 2009-01-19 | 2009-07-08 | 北京林业大学 | Method for tissue separation of edible fungus |
| CN102172173A (en) * | 2011-03-04 | 2011-09-07 | 广东省微生物研究所 | Method for cultivating and producing sporocarps of dictyophora multicolor berk. and broome |
Non-Patent Citations (2)
| Title |
|---|
| 罗国涛: "金针菇原基分离培养菌种试验", 《食用菌》, no. 03, 30 June 1995 (1995-06-30) * |
| 蒋中海等: "食用菌菌质分离的研究", 《生物技术》, no. 05, 20 October 1991 (1991-10-20) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488557A (en) * | 2014-12-26 | 2015-04-08 | 青岛华盛绿能农业科技有限公司 | Method for purification and rejuvenation of edible fungus strain |
| CN106386161A (en) * | 2015-08-11 | 2017-02-15 | 天津市庆祥农业科技有限公司 | Method for preparing edible fungus strains |
| CN105393792A (en) * | 2015-11-12 | 2016-03-16 | 黄福忠 | Method for taking strain and inoculation of strain from bottom of original strain bag |
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
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Application publication date: 20130821 |