CN1796536A - Purifying polluted parent edible fungus - Google Patents
Purifying polluted parent edible fungus Download PDFInfo
- Publication number
- CN1796536A CN1796536A CN 200410075594 CN200410075594A CN1796536A CN 1796536 A CN1796536 A CN 1796536A CN 200410075594 CN200410075594 CN 200410075594 CN 200410075594 A CN200410075594 A CN 200410075594A CN 1796536 A CN1796536 A CN 1796536A
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- CN
- China
- Prior art keywords
- suppress
- edible fungi
- edible fungus
- penicillium notatum
- polluted
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This invention describes a process for the purification of polluted edible fungi, comprising the steps of: adding 40,000 unit gentamycin into a PDA inclined-plane culture medium to suppress the polluting bacteria; adding nystafungin tablets into the PDA inclined-plane culture medium to suppress the pollutiong melanomyces; immersing filter paper into formaldehyde or methanol and then covering on the penicillium notatum colony to suppress enicillium notatums, thus obtaining purified high-yield, high-quality edible fungi. The purified edible fungi reserve all the nutrients of the previous fungi as well as such advantages as high yield, high quality, delicious and tender. The process effectively resolves the problem of edible fungi's pollution.
Description
Technical field: the present invention relates to a kind of purification process of polluted parent edible fungus, belong to edible fungus species purification and rejuvenation field.
Background technology: edible mushrooms will expect that high yield, stable yields must have good pure parent edible fungus kind, since when mother plants tube or tampon when moist or medium sterilization not thorough, strain excellent is contaminated bacterium or mould, current do not have a kind of ideal method of purification.In order to obtain pure bacterial strain, the invention provides under three kinds of situations and handle, can obtain the pure female bacterial strain of planting of edible mushrooms.
Summary of the invention: but the present invention is characterised in that bacterial contamination purification, Penicillium notatum pollute purification, melanomyces pollutes and purifies.
Embodiment: the present invention realizes by following technical proposal:
Embodiment 1
(1) material: content is one of the gentamicin of 40,000 units/1 milliliter; Through 10 of autoclaved PDA slant mediums (18 * 180 millimeters of test tube specifications), one of autoclaved 1 milliliter of syringe; No. 6 pins first.
(2) method: in sterile cupboard, the gentamicin of 40,000 units/1 milliliter is also severed with emery wheel, injected 10 slant tube substratum that are cooled to about 45 ℃ respectively with asepsis injector, the wave and culture base makes it abundant mixing, puts into the inclined-plane immediately.Being moved in this slant medium with the aseptic technique method, put 25 ℃ and cultivate down then by the bacterial classification of bacterial contamination.Can get rid of bacterium for 2 times, effect is excellent, to hypha of edible fungus unrestraint effect.Can carry out bacterial contamination purifies.
Embodiment 2
(1) the test tube tampon makes moist and has polluted Penicillium notatum, mostly appears at the inclined-plane front end, can burn together with substratum one fast Shuo on spirit lamp flame, makes local heating bake dead mould.Test tube is latter half of with wet gauze parcel, tube then.
(2) Penicillium notatum occurs in the middle of the inclined-plane, with a filter paper bigger slightly than mould, drip and go up formaldehyde or alcohol, cover gently on mold colony, can limit the Penicillium notatum growth, it is long slightly to treat that hypha of edible fungus stretches, and tube promptly obtains the purifying mycelia at once.Also can provoke a spot of mycelia, be transplanted to new slant medium front end, when mycelium germination outwards spreads, in time will plant piece and dig out together with substratum from polluting an end far away.Handle 2～3 times continuously, can get the purifying mycelia.The Penicillium notatum of can purifying pollutes.
Embodiment 3
Occur melanomyces in the inclined-plane, also can arrive pharmacy and buy the nystatin tablet, tablet is placed in the sterilized water removes sugar-coat for a moment, then, in being placed on the little triangular flask that fills 10 milliliters, equally operate with (2) of bacterial contamination method.Can get rid of melanomyces for 2 times.Can get pure edible mushroom strains.
Claims (3)
1, it is by adding the gentamicin of 40,000 units, the bacterium that can suppress to pollute in the PDA slant medium;
2, in the PDA slant medium, add nystatin tablet, the melanomyces that can suppress to pollute;
3, immerse formaldehyde or alcohol on filter paper, covering on the Penicillium notatum bacterium colony gently can suppress Penicillium notatum.
Use mother multi-form, that edible mushrooms is polluted to plant and handle, thereby purify out your original pure bacterial strain.Can obtain the pure edible mushroom strains of original good quality and high output good character.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410075594 CN1796536A (en) | 2004-12-22 | 2004-12-22 | Purifying polluted parent edible fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410075594 CN1796536A (en) | 2004-12-22 | 2004-12-22 | Purifying polluted parent edible fungus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1796536A true CN1796536A (en) | 2006-07-05 |
Family
ID=36817856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410075594 Pending CN1796536A (en) | 2004-12-22 | 2004-12-22 | Purifying polluted parent edible fungus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1796536A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
-
2004
- 2004-12-22 CN CN 200410075594 patent/CN1796536A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220560A (en) * | 2018-11-19 | 2019-01-18 | 遵义周星星菌业有限公司 | A kind of test tube of Needle mushroom strain is without base and has base critical point breeding, method of purification |
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Legal Events
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |