CN107926478A - A kind of bacterial strain of suitable factory culture white gold needle mushroom and its application - Google Patents
A kind of bacterial strain of suitable factory culture white gold needle mushroom and its application Download PDFInfo
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- CN107926478A CN107926478A CN201710972612.0A CN201710972612A CN107926478A CN 107926478 A CN107926478 A CN 107926478A CN 201710972612 A CN201710972612 A CN 201710972612A CN 107926478 A CN107926478 A CN 107926478A
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Abstract
The present invention provides a kind of bacterial strain of suitable factory culture white gold needle mushroom, Classification And Nomenclature is needle mushroom（Flammulina velutipes）CG FVDA12, have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No. 12971.Present invention also offers the method using above-mentioned Strains of Flammulina velutipes CG FVDA12 factory culture white gold needle mushrooms.For the Strains of Flammulina velutipes CG FVDA12 of the present invention under the conditions of factory culture, the fructification after fruiting has the advantages that mushroom body white color, regularity height, cap rounding are not easy parachute-opening, to cut root back root part white, can be widely popularized use.
Description
Technical field
The invention belongs to edible fungus industrial cultivation technical field, and in particular to a kind of suitable factory culture white acupuncture needle
The bacterial strain of mushroom and its application.
Background technology
Needle mushroom is the fruiting performances at low temperature edible mushroom occurred in a kind of autumn end to early spring, is in plexi when wild, and needle mushroom has
The characteristics of stem is tender and crisp, cap sticks sliding, delicious flavour, rich in amino acid, be otherwise known as " increasing intelligence mushroom ", and needle mushroom has yellow and white
Two strains of color, compared with Yellow strain, the exterior of commodity of White strain is more preferable, therefore is subject to market to welcome, existing market pin
The most of product for Industrialized mode cultivation of white sold.Compared with traditional cultivation, factory culture pattern using it is various from
Dynamicization equipment, the natural growing environment of artificial creation climatic simulation edible mushroom, realize the standardization of edible mushroom, mechanization, from
Dynamic metaplasia production, the needle mushroom of production is more guaranteed in food security aspect, therefore is loved by consumers.Strain is needle mushroom work
Factoryization cultivation it is crucial and basic, strain quality excellent and stablize it is final have decided on whether can stable high yield, but current state
The Varieties in Flammulina velutipes common manifestation inside used is bad, easily variation, and very big influence is caused to the stability of per unit area yield and production.
The content of the invention
Bacterial strain and its application, the bacterial strain the object of the present invention is to provide a kind of suitable factory culture white gold needle mushroom exist
Under the conditions of factory culture, there is the fructification after fruiting mushroom body white color, regularity height, cap rounding to be not easy parachute-opening, cut root
The advantages of back root part is white, can be widely popularized use.
The present invention provides a kind of bacterial strain of suitable factory culture white gold needle mushroom, its Classification And Nomenclature is needle mushroom
(Flammulina velutipes) CG-FVDA12, was preserved in Chinese microorganism strain preservation pipe on 09 21st, 2016
Reason committee common micro-organisms center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, its deposit number are CGMCC No.12971.
Needle mushroom (Flammulina velutipes) the bacterial strain CG-FVDA12 is by needle mushroom (Flammulina
Velutipes spore) is obtained after bacterial strain CG-FVDA001 fruitings, is obtained after crossbreeding.
Needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA001, on 07 12nd, 2012 are preserved in
State's Microbiological Culture Collection administration committee common micro-organisms center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology of the academy of sciences of state, its deposit number are CGMCC No.6343.
The biological characteristics of needle mushroom (Flammulina velutipes) the bacterial strain CG-FVDA12 is as follows：
(1) colony morphology characteristic
White mycelium, bacterium colony are in thin velveteen shape, slightly climb wall phenomenon, and during mycelia aging, bacterium colony surface is in khaki.
(2) mycelia microscopic features
Under microscopy conditions, mycelia even thickness, nucleated mycelium has typical clamp connection structure, and uninucleate hyphae is without lock shape
Joint.
(3) sporophore shape feature
Fructification is grown thickly, and mature sporophore is made of cap, lamella, stem three parts.Cap pure white, diameter 1.0cm
~1.5cm, initial stage are hemispherical, rear gradually open and flat, cap surface stick-slip during moistening.Lamella white or near-white.Stem is pure white
Color, long 15~17cm, diameter 2mm~4mm, base portion have white flock hair, and medulla enriches inside initial stage, and the later stage becomes hollow.Spore
Son print white.
(4) demand of the mycelia to temperature
Most fast 22~25 DEG C of the temperature of mycelial growth, optimum temperature are 18~20 DEG C.It is 13~15 DEG C that temperature, which occurs, for former base, son
Entity developmental temperature is 4~13 DEG C.
(5) demand of the mycelia to acid-base value
The bacterial strain cultural hypha optimal pH is 6.0~6.5.
Needle mushroom (Flammulina velutipes) the bacterial strain CG-FVDA12 is in factory culture white gold needle mushroom
Using comprising the following steps：
Step 1, liquid spawn is prepared with needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA12, implantation is planted
It is inoculated with training bottle, every bottle of inoculum concentration is 25~30mL；
Step 2, the culture bottle for connecting liquid spawn is moved into culturing room, according to needle mushroom factory production process control ring
Border condition carries out bacterium germination culture, and fertility room progress fruiting is moved into again after the culture bottle after bacterium germination is carried out mycelium stimulation processing, you can.
The present invention needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA12 under the conditions of factory culture,
Fructification after fruiting has the advantages that mushroom body white color, regularity be high, cap rounding is not easy parachute-opening, it is white to cut root back root part, can
It is widely popularized use.
Brief description of the drawings
Fig. 1 is the opposite culture front of CG-FVDA12 and CG-FVDA001 antagonistic experiments in embodiment 1；
Fig. 2 is the opposite culture back side of CG-FVDA12 and CG-FVDA001 antagonistic experiments in embodiment 1；
Fig. 3 is the process flow chart that CG-FVDA12 factory cultures are tested in embodiment 2；
Fig. 4 is the harvesting situation of CG-FVDA12 in embodiment 2；
Fig. 5 is that CG-FVDA12 and CG-FVDA001 harvests contrast situation in embodiment 2；
Wherein, 1 it is CG-FVDA12,2 is CG-FVDA001.
Embodiment
With reference to embodiment, the present invention is described in further detail.
The formula of PDA culture medium is used in following embodiments：Per 1L culture mediums potato 200g, glucose 20g, agar
20g。
Embodiment 1
The selection of needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA12
Needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA001 fruitings under the conditions of batch production, collect spore
Son, is diluted to spore suspension with sterile water by spore, is uniformly coated on PDA solid mediums, and 18~20 DEG C of cultures, spore is sprouted
After hair forms uninucleate hyphae, isolating and purifying for uninucleate hyphae is carried out under the microscope, monokaryon can be obtained after 18~20 DEG C of cultures
More plants of mononuclear bacterial strains of acquisition are matched cross experiment, after the contact of two uninucleate hyphaes, place one by mycelia pure culture two-by-two
The section time, the contact site of both pickings, examines under microscope, has the explanation successful matching of clamp connection, picking culture block connects
Kind on the 90mm culture dishes containing PDA solids, during a diameter of 3cm~4cm of mycelium, the single double-core bacterium of picking under the microscope
Silk tip, is purified.Multiple pure culture double-core bacterial strains of acquisition are carried out continuously multiple biological characteristics experiment and fruiting examination
Screening is tested, strain excellent CG-FVDA12 is selected by synthetical comparison and assessment.
The batch production condition and needle mushroom of needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA001 fruitings
The factory culture experiment of (Flammulina velutipes) bacterial strain CG-FVDA12 is identical.
The PDA strain blocks of strain excellent CG-FVDA12 and bacterial strain CG-FVDA001 are inoculated into the 90mm containing solid PDA
In culture dish, antagonistic experiment is carried out, as depicted in figs. 1 and 2, in the front and back of opposite culture bacterium colony intersection, CG-
FVDA12 and CG-FVDA001 antagonism lines are obvious, illustrate that CG-FVDA12 from CG-FVDA001 is different Strains of Flammulina velutipes.
To verify the biological characteristics of bacterial strain CG-FVDA12, related experiment is carried out, concrete outcome is as follows：
1. cultivating utensil temperature experiment, bacterial strain CG-FVDA12 is inoculated into solid PDA culture dishes, different temperature is set
Gradient, is cultivated, and measures mycelial growth rate in time, as a result such as following table：
2. culture dish pH is tested, bacterial strain CG-FVDA12 is inoculated into solid PDA culture dishes, PDA culture medium is set when configuring
Different pH gradients is put, is cultivated under the conditions of 18 DEG C, measures mycelial growth rate in time, as a result such as following table：
3. culture material pH is tested, bacterial strain CG-FVDA12 is inoculated into solid medium, different pH gradients is set, trained
Based formulas is supported as corncob 35%, rice bran 39%, wheat bran 15%, megasse 5%, corn flour 5%, oyster shell whiting is added according to pH,
Moisture content 68%, is cultivated after inoculation under the conditions of 18 DEG C, measures mycelial growth rate in time, as a result such as following table：
Embodiment 2
The factory culture experiment of needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA12, specific steps are such as
Under：
First, the preparation of liquid spawn
1st, using needle mushroom (Flammulina velutipes) bacterial strain CG-FVDA12 of screening as strain, PDA examinations are inoculated into
Cultivated in pipe culture medium, under the conditions of 18 DEG C 8 days and expand culture；
2nd, access liquid triangular flask culture medium in, be formulated for 20g level-one white granulated sugar+3g200 mesh dregs of beans+0.6g magnesium sulfate+
0.6g potassium dihydrogen phosphate+1L purified waters, pH6.0,18 DEG C of shake cultures 9 days；
3rd, in shaking flask strain access fermentation tank culture medium, it is formulated as 12Kg level-one white granulated sugar+1.5Kg200 mesh dregs of beans+300g
Magnesium sulfate+300g potassium dihydrogen phosphate+30mL defoamer+700L pure water, pH6.0,18 DEG C culture 9 days after liquid spawn is made；
2nd, factory culture is tested
1st, by formula (corncob 35%, rice bran 39%, wheat bran 15%, megasse 5%, corn flour 5%, oyster shell whiting 1%,
According to the final moisture content 68% of culture medium plus water) culture medium is prepared, add water by automatic bottling machine to load volume after stirring
In 1200mL plastics culture bottles, bottle cap is covered；
2nd, culture bottle is subjected to high pressure steam sterilization；
3rd, force to be cooled to 18 DEG C or so under purification condition in the culture bottle after sterilizing, aseptically with automatic vaccination
Liquid spawn is inoculated into culture bottle by machine, and every bottle of inoculum concentration is 25~30mL；
4th, the culture bottle for connecting liquid spawn is moved into culturing room, under 18 DEG C of temperature, 60% air humidity, dark condition
Bacterium germination culture is carried out, i.e. bacterium germination expires bottle within 19 days or so；
5th, the culture bottle after bacterium germination is subjected to mycelium stimulation processing in the 21st day, removes the old mycelia of charge level, and form mechanical stimulus,
Then by culture bottle move to fertility room, 15~17 DEG C, air humidity 85%~95%, CO2Concentration is in 3000~4000ppm conditions
Lower flower bud, charge level is covered with mushroom flower bud after 4~6 days, and Xanthophyll cycle (intensity of illumination 100Lux) is carried out to mushroom flower bud and wind suppresses (blowing speed
Rate is 1~3m/s, air quantity 150m3/ h), by CO2Concentration control is in 3000~8000ppm, and gradient cooling is to 5~8 DEG C, mushroom flower bud
Grow into mushroom bud, during the long 1~2cm of bottle outlet of mushroom bud, wrap plastic bag mushroom piece, improve CO2Concentration promotes to more than 10000ppm
The elongation of needle mushroom stem；
6th, when needle mushroom grows to 16~17cm, reach harvesting height, harvested.
Concrete technology flow process is shown in Fig. 3.
CG-FVDA12 bacterial strains set the output in 3 storehouses altogether, and the strain is in cultivation stage mycelia mycelium growing period and CG-
FVDA001 differences are little, and early period, mycelium germination was very fast, and mycelia is dense compared with MLCG-FVDA001, and charge level has a small amount of physiology within the 4th day
Water, then growth are slowed down, and later stage growing way and CG-FVDA001 are basically identical, and the full bottle time at 19 days or so, carried out in the 21st day
Mycelium stimulation.
The harvesting situations of CG-FVDA12 bacterial strains as shown in figure 4, the harvesting of CG-FVDA12 and CG-FVDA001 to such as Fig. 5
It is shown.CG-FVDA12 bacterial strains are analyzed from yield and quality, in terms of yield：3 storehouses of CG-FVDA12 bacterial strains are averaged per unit area yield about
450g, than MLCG-FVDA001 high 10g or so；In terms of quality：CG-FVDA12 bacterial strains are less relatively thick in fertility room stage bud number,
Cut that root back root part whiteness is good, and the degree of packing is preferable, mushroom type is good, and regularity is high, mushroom cap white color, and uniformity is good.
Claims (5)
1. a kind of bacterial strain of suitable factory culture white gold needle mushroom, Classification And Nomenclature is needle mushroom（Flammulina velutipes）CG-FVDA12, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation is compiled
Number it is CGMCC No. 12971.
2. the bacterial strain of the suitable factory culture white gold needle mushroom described in claim 1 should factory culture white gold needle mushroom
With.
3. application according to claim 2, it is characterised in that：By needle mushroom（Flammulina velutipes）Bacterial strain
CG-FVDA12 activation after successively bacterium germination culture, mycelium stimulation processing and fruiting, you can.
4. application according to claim 3, it is characterised in that：The culture medium prescription of bacterium germination culture is：Corncob 35%, rice
Chaff 39%, wheat bran 15%, megasse 5%, corn flour 5%, oyster shell whiting 1%, by final moisture content 68% plus water.
5. application according to claim 3, it is characterised in that：The condition of bacterium germination culture is 18 DEG C, 60% air humidity, black
Dark condition.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111466255A (en) * | 2020-03-27 | 2020-07-31 | 江苏华绿生物科技股份有限公司 | Method for rejuvenating flammulina velutipes strains by using wood chip culture medium |
| CN112266878A (en) * | 2020-08-29 | 2021-01-26 | 福建农林大学 | Flammulina velutipes suitable for industrial cultivation and molecular identification method thereof |
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| CN101366346A (en) * | 2008-07-31 | 2009-02-18 | 芜湖野树林生物科技有限公司 | Clear-white gold needle mushroom cultivation method |
| CN102986536A (en) * | 2012-12-02 | 2013-03-27 | 中华全国供销合作总社昆明食用菌研究所 | Flammulina velutipes strain and preparation method |
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| CN101366346A (en) * | 2008-07-31 | 2009-02-18 | 芜湖野树林生物科技有限公司 | Clear-white gold needle mushroom cultivation method |
| CN102986536A (en) * | 2012-12-02 | 2013-03-27 | 中华全国供销合作总社昆明食用菌研究所 | Flammulina velutipes strain and preparation method |
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| CN111466255A (en) * | 2020-03-27 | 2020-07-31 | 江苏华绿生物科技股份有限公司 | Method for rejuvenating flammulina velutipes strains by using wood chip culture medium |
| CN112266878A (en) * | 2020-08-29 | 2021-01-26 | 福建农林大学 | Flammulina velutipes suitable for industrial cultivation and molecular identification method thereof |
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