CN103272065A - Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil - Google Patents
Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil Download PDFInfo
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Abstract
The invention discloses a method for extracting a volatile oil from agilawood seeds, and a usage and an application of the volatile oil. In the method disclosed by the invention, a supercritical CO2 fluid extraction method is adopted to extract volatile components from aquilaria sinensis seeds; and a DPPH method is used to study anti-oxidation capability of effective components of the volatile components. With the method, squalene with relatively high content is detected in the aquilaria sinensis seeds for the first time; and the volatile oil has a certain anti-oxidation capability, so that scientific basis is provided for further studying active components of the aquilaria sinensis seeds development and utilization The extraction method is simple in process flow, high in extraction yield and low in extraction temperature, and can prevent degradation of heat-sensitive components. The method fully develops rich resources of the agilawood rare medicinal material, and makes the best use of the agilawood. With the method, effective components in the agilawood seeds are extracted simply and effectively, thereby developing novel raw materials and extraction process for the squalene.
Description
[technical field]
The present invention relates to a kind of extracting method of Lignum Aquilariae Resinatum seed extract, particularly a kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed the invention still further relates to purposes and the application of this Lignum Aquilariae Resinatum seed extract volatile oil, belongs to biological medicine, health care technology field.
[background technology]
The resiniferous timber of Lignum Aquilariae Resinatum Aquilaria sinensis (lour.) Gilgl. is called Lignum Aquilariae Resinatum, is called homemade Lignum Aquilariae Resinatum, TUCHENXIANG or tabernaemontanus bulrush perfume (or spice) again, and Lignum Aquilariae Resinatum also is unique plant resources that China produces the Lignum Aquilariae Resinatum medical material.The wild resource of Lignum Aquilariae Resinatum is endangered, now has been listed in the rare three-level protective plant in imminent danger of country and national secondary and has laid special stress on protecting plant.Lignum Aquilariae Resinatum, acrid in the mouth, hardship, warm in nature, have the effect of promoting the circulation of QI to relieve pain, warming middle-JIAO to arrest vomiting, helping inspiration to relieve asthma, be used for the treatment of the vexed pain of breast abdominal distention, gastrofrigid vomiting singultus, the QI rising in reverse order of suffering from a deficiency of the kidney dyspnea with rapid respiration, be the traditional precious Chinese medicine of China.
Lignum Aquilariae Resinatum is high megaphanerophyte, poor growth, and the Edgeworthia chrysantha Lindl. cycle is long, needs 5 years above diameters of a cross-section of a tree trunk 1.3 meters above the ground of the age of tree to reach 15cm and just can manually make perfume (or spice).And Lignum Aquilariae Resinatum can be yielded positive results in plantation in 3 years, and a large amount of seeds of annual generation only have the small part seed to be used for growing seedlings at present, and its affluent resources are also effectively developed.Discover that the Lignum Aquilariae Resinatum seed has certain methicillin-resistant staphylococcus aureus resistance (MRSA) activity, and the exploitation of its other medicinal parts and the comprehensive utilization of resource be yet there are no report.
Squalene has another name called zamene, squalene, the cod-liver oil terpene, it is a kind of height unsaturated hydrocarbons compounds, in black shark liver oil, found in 1906 by Japanization scholar Tsujimoto at first, Squalene is a kind of lipid non-saponifiable matter, claim the cod-liver oil terpene again, has superoxide dismutase (SOD) activity in the body of raising, the enhancing human body immunity ability, sexual function improving, defying age, resisting fatigue, different physiological roles such as antitumor, Squalene has extremely strong oxygen delivery capacity, but anticancer generates, prevent cancerous cell diffusion and because of the chemotherapy leukopenia, also has resisting fatigue simultaneously, anti-cardiovascular disease, the anti-infective effect is a kind of avirulent marine bioactivity material with the effect of preventing and curing diseases.Squalene distributes very wide in plant, but content is not high, is lower than 5% of non-saponifiable matter in the vegetable oil more, and only minority content is more, contains Squalene 150-700mg/100g as olive oil, and Testa oryzae oil content is 332mg/100g; Oleum Camelliae is the general name to oily substance in the Semen Camelliae, also contains more Squalene in the Oleum Camelliae.Because the content height of Squalene is 40%-74% as content in the armour shark-liver oil in the shark liver of deep-sea, the spinule shark contains 49%-89%, therefore, mostly adopts the deep-sea shark liver oil to extract Squalene at present; But so the mass production Squalene can cause the disruption of ecological balance of deep-sea shark.
Also have a small amount of method of extracting Squalene from plant to announce in recent years, Chinese patent (CN201110027119.4) " be method and Oleum Camelliae Squalene soft capsule that raw material extract Squalene with the Semen Camelliae " is to utilize lixiviate, chromatographic isolation and room temperature extraction and several extracting method of supercritical extraction in conjunction with extracting the high-purity squalene that is suitable for; Chinese patent (CN200910069670.8) " being the method that raw material extracts high-purity squalene with the olive oil " is that the separating technology with secondary molecular distillation and silica gel chromatography combination extracts high-purity squalene; The process that Chinese patent (CN201010190275.8) " method of extraction Squalene from plant oil deodorizing distillate " utilizes molecular distillation to be combined with extraction, crystalline phase is extracted the high concentration Squalene; These methods all need multiple extracting method to be used in combination, and technology is more complicated all, and introducing has other chemical substances, difficult control during operating procedure.
Contain abundant Squalene in the Lignum Aquilariae Resinatum seed, being expected to exploitation becomes medicine, cosmetics and health food etc.
[summary of the invention]
Purpose of the present invention is just in order to remedy the blank and not enough of prior art, provide a kind of technological process simple, the extraction efficiency height, extraction temperature is low, can avoid the method for from the Lignum Aquilariae Resinatum seed, extracting volatile oil of thermal sensitivity composition degraded, open up the development approach at new drug usefulness position in the Lignum Aquilariae Resinatum seed.
Another object of the present invention provides purposes and the application of volatile oil in a kind of above-mentioned Lignum Aquilariae Resinatum seed.
The present invention to achieve these goals, by the following technical solutions:
A kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed is characterized in that adopting supercritical carbon dioxide to extract.
A kind of method of from the Lignum Aquilariae Resinatum seed, extracting volatile oil of the present invention, it may further comprise the steps:
Get dry Lignum Aquilariae Resinatum seed, with pulverizer it is pulverized, place supercritical extraction reactor with the CO of 10-30L/h then
2At 40～50 ℃, extract 1.5～2.5h under 18～25MPa condition, will extract the back fluid then and carry out decompression separation collection extract twice, namely get volatile oil.
A kind of method of from the Lignum Aquilariae Resinatum seed, extracting volatile oil of the present invention, twice decompression separation wherein comprises first separating still, pressure 5～7MPa, temperature is 45～50 ℃ and second separating still, pressure 5～7MPa, 45～50 ℃ of temperature.
The present invention is a kind of to extract from the Lignum Aquilariae Resinatum seed in the method for volatile oil, and the Lignum Aquilariae Resinatum seed is pulverized the back and crossed 20～40 mesh sieves.
The present invention is a kind of to extract in the method for volatile oil the water content of dry Lignum Aquilariae Resinatum seed≤10% from the Lignum Aquilariae Resinatum seed
Effective ingredient in the volatile oil that the present invention extracts is Squalene.
The volatile oil that the present invention extracts from the Lignum Aquilariae Resinatum seed is used for removing the purposes of DPPH free radical.
The application preparation of the volatile oil that the present invention extracts from the Lignum Aquilariae Resinatum seed is soft capsule.
This soft capsule was mixed with the uniform liquid filling by volatile oil, vitamin E and soybean oil when the application preparation of the volatile oil that the present invention extracts from the Lignum Aquilariae Resinatum seed was soft capsule.
Adopt supercritical CO of the present invention
2Volatile oil in the fluid extraction Lignum Aquilariae Resinatum seed has following advantage:
Technological process is simple, the extraction efficiency height, extraction temperature is low, can avoid the degraded of thermal sensitivity composition, the present invention detects the higher Squalene of content first in the volatile oil of Lignum Aquilariae Resinatum seed, this volatile oil has certain oxidation resistance, for further its active component of research and development and use provide scientific basis.
The present invention adopts the annual superfluous a large amount of seeds of Lignum Aquilariae Resinatum, the affluent resources of this valuable ingredient of Chinese medicine of Lignum Aquilariae Resinatum have fully been developed, accomplish and make the best use of everything that the present invention has extracted the effective ingredient in the Lignum Aquilariae Resinatum seed simply and effectively, for Squalene has been opened up new raw material and extraction process.
Squalene content height in the volatile oil that the present invention extracts, its content accounts for 41.345% of volatile ingredient, and other subsidiary component contents are lower, do not influence the effectiveness performance of Squalene composition, also are easy to separate.
The volatile oil that the present invention extracts has extremely strong oxygen delivery capacity, but the anticancer generation prevents the cancerous cell diffusion and because of the chemotherapy leukopenia, also has resisting fatigue, anti-cardiovascular disease, anti-infective effect simultaneously.Contain abundant Squalene in the Lignum Aquilariae Resinatum seed, being expected to exploitation becomes medicine, cosmetics and health food etc.
Technology of the present invention is simply single, and is easy to control, do not introduce new impurity in the volatile oil that extracts, and original other compositions do not influence the use of main functional component Squalene yet.
On the other hand, it is 68.5% that the Lignum Aquilariae Resinatum of discovery seed oil content is arranged, seed far above other xylophytas, have the potentiality as oil plant or biodiesel resource, the present invention effectively do not introduce again new impurity extraction the volatile oil in the Lignum Aquilariae Resinatum seed, for new way has been opened up in the abundant development and utilization of Lignum Aquilariae Resinatum seed.
[description of drawings]
Fig. 1 is Lignum Aquilariae Resinatum seed volatile oil GC-MS analyzing total ion flow;
Fig. 2 is the absorption spectrum curve figure of DPPH;
Fig. 3 is volatile oil sample solution trap curve chart over time;
Fig. 4 is that Lignum Aquilariae Resinatum seed volatile oil is removed the bar diagram of DPPH free radical ability.
[specific embodiment]
The invention will be further described below in conjunction with the specific embodiment:
One, instrument and medical material
Material: medical material is Lignum Aquilariae Resinatum Aquilaria sinensis (lour.) the Gilgl. seed of planting base, Lignum Aquilariae Resinatum mountain, Maoming, is accredited as certified products through the author.
Instrument: gas phase-mass spectrometry chromatograph is the Agilent6890-5973N type of U.S. Agilent company; Supercritical extraction equipment is the HA121-50-05 of Jiangsu Huaan Science Research Instrument Co., Ltd model; Ultraviolet spectrophotometer is day UV-2550 model of island proper Tianjin instrument company.
Reagent: DPPH (1,1-Dipheyl-2-picrylhydrazyl radicle, 1,1-diphenyl-2-picryl hydrazine) Aladdin reagent (Shanghai) Co., Ltd.; Dehydrated alcohol Shantou Xilong Chemical Co., Ltd is analytical pure.
Two, extracting method:
When the present invention extracts the Lignum Aquilariae Resinatum seed and waves oil, get dry Lignum Aquilariae Resinatum seed, with pulverizer it was crushed to 20～40 mesh sieves, place supercritical CO then
2In the extraction kettle with the flow of 20L/h, at 40～50 ℃, extract 1.5～2.5h under 18～25MPa condition, then respectively with the first separating still pressure, 5～7MPa, 45～50 ℃ of temperature, the second separating still pressure, 5～7MPa, 45～50 ℃ of collections of temperature have the yellow transparent grease of strong fragrance, i.e. volatile oil.
Three, chemical composition analysis
The GC-MS analysis condition
Chromatographic condition:
Chromatographic column Agilent HP-5MS (0.25umx250umx30m), injector temperature are 250 ℃, and carrier gas is He gas, and the post flow is 1.0ml/min, the temperature programming condition: 50 ℃ of initial temperatures, rise to 220 ℃ with 4 ℃/min, and the back rises to 300 ℃ with 10 ℃/min.
The mass spectrum condition:
Normal pictures is tuning, and ion source temperature is 230 ℃, and level Four bar temperature is 150 ℃, and the data acquisition scan pattern is full scan, solvent delay time 3min.
Adopt gas chromatograph-mass spectrometer (GC-MS) (GC-MS) that Lignum Aquilariae Resinatum seed ethereal oil is separated and content analysis, by above-mentioned analysis condition, its gas chromatography-mass spectrum (GC-MS) total ion current figure as shown in Figure 1, vertical coordinate is represented abundance, the abscissa express time, gained chromatograph and mass spectrum information are retrieved and manual retrieval, contrast and parsing automatically through NIST2011 standard spectrum storehouse, identify the chemical constituent in the Lignum Aquilariae Resinatum volatile oil, determine the mass fraction of each composition with area normalization method.
This experimental identification 9 main compound in the Lignum Aquilariae Resinatum volatile oil, the composition majority is fatty acid, content is higher Squalene squalene(41.345%), ethyl oleate ethyl oleate(32.233%) and n-hexadecanoic acid(16.708%) etc., concrete analysis the results are shown in Table 1:
Table 1: Lignum Aquilariae Resinatum chemical composition of volatile oil analysis result C
14
Four, the mensuration of volatile oil oxidation resistance
The preparation of DPPH solution:
Precision takes by weighing in 0.0198g DPPH to the 50ml volumetric flask, is settled to graduation mark with dehydrated alcohol, as storing solution.The time spent precision pipettes in storing solution 10ml to the 100ml volumetric flask, obtains the DPPH working solution of 0.1mmol/L with the dehydrated alcohol standardize solution.
The preparation of sample solution:
With dehydrated alcohol Lignum Aquilariae Resinatum seed volatile oil is mixed with low concentration group and high concentration group, low concentration group is respectively: 2,4,6,8,10mg/mL sample solution; The high concentration group is respectively: 20,40,60,80,100mg/mL sample solution.
Determining of maximum absorption wavelength:
Make DPPH solution in 280～700nm range of wavelengths, carry out spectral scan, can find that DPPH all has strong absworption peak at 326nm and 516nm place, after adding sample solution, these two peak values all have reduction, and the reduction of the peak value at 516nm wavelength place is comparatively remarkable, as shown in Figure 2, therefore, choose 516nm as measuring wavelength.
Determining of response time:
Get the 1ml20mg/mL sample solution and join in the 7ml DPPH solution, mix homogeneously is surveyed its absorbance (A value) every 5min, and is basicly stable up to the A value.The solution A value over time rule as shown in Figure 3, as seen from Figure 3, in adding the 20min of sample, absorbance descends very fast, and along with the increase of time, the downward trend of absorbance tends towards stability gradually, it is stable that the 40min afterreaction reaches substantially, therefore selects 40min as the response time.The antioxidation of fixation response time is measured:
The sample solution that pipettes the 2ml variable concentrations respectively adds in the DPPH solution of 4ml, mix homogeneously, and room temperature is placed 40min, measures its absorbance (A), parallel assay 3 times at maximum absorption wavelength 516nm place; With the DPPH solution that adds the 2ml dehydrated alcohol as blank (A
0).At last calculate each concentration sample to the clearance rate of DPPH free radical according to following formula: clearance rate (ρ)=1-A/A
0* 100%.
The DPPH molecule can generate the stable nitrogen free radical that contains in solution, solution colour shows purple, does the time spent when it and free radical scavenger, can generate colourless product, and solution colour is shoaled.Different antioxidants is different to the clearance rate of free radical with removing effect, and clearance rate can reflect that to measured by esr technique clearance rate is more big, and antioxidant activity is just more strong, otherwise then more weak.
The Lignum Aquilariae Resinatum volatile oil of variable concentrations is removed the contrast of DPPH free radical ability and is seen shown in Figure 4, the result shows, the volatile oil of Lignum Aquilariae Resinatum seed has certain removing ability to the DPPH free radical, high concentration group clearance rate is apparently higher than low concentration group, and along with the increase of concentration, clearance rate increases gradually, when concentration is 80mg/mL, clearance rate reaches 82.8%, and along with concentration increases, clearance rate increases not obvious afterwards.
Five, following is several specific embodiment of the present invention
Embodiment 1:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 40 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 40 ℃, extract 1.5h under the 25MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 6MPa, 50 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 132g, extraction yield is 44%, and the extraction ratio of Squalene is 16.72%.
Embodiment 2:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 30 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 40 ℃, extract 2h under the 18MPa condition, then respectively at first separating still (pressure 6MPa, 48 ℃ of temperature) with second separating still (pressure 5MPa, 48 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 117g, extraction yield is 39%, and the extraction ratio of Squalene is 14.04%.Embodiment 3:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 20 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 40 ℃, extract 2.5h under the 21MPa condition, then respectively at first separating still (pressure 7MPa, 45 ℃ of temperature) with second separating still (pressure 5MPa, 45 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 147g, extraction yield is 49%, and the extraction ratio of Squalene is 19.11%.
Embodiment 4:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 20 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 45 ℃, extract 1.5h under the 18MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 6MPa, 48 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 105g, extraction yield is 35%, and the extraction ratio of Squalene is 14.7%.
Embodiment 5:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 40 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 45 ℃, extract 2h under the 21MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 5MPa, 45 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 189g, extraction yield is 63%, and the extraction ratio of Squalene is 25.83%.
Embodiment 6:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 30 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 45 ℃, extract 2.5h under the 25MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 5MPa, 45 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 138g, extraction yield is 46%, and the extraction ratio of Squalene is 19.32%.
Embodiment 7:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 30 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 50 ℃, extract 1.5h under the 21MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 7MPa, 50 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 162g, extraction yield is 54%, and the extraction ratio of Squalene is 20.52%.
Embodiment 8:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 20 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 50 ℃, extract 2h under the 25MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 5MPa, 45 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 138g, extraction yield is 46%, and the extraction ratio of Squalene is 16.56%.Embodiment 9:
Get the dry Lignum Aquilariae Resinatum seed of water content≤10%, with pulverizer it was crushed to 40 mesh sieves, accurately take by weighing 300g and place extraction kettle with the CO of 20L/h then
2At 50 ℃, extract 2.5h under the 18MPa condition, then respectively at first separating still (pressure 7MPa, 50 ℃ of temperature) with second separating still (pressure 5MPa, 45 ℃ of temperature) the yellow transparent grease of strong fragrance is collected in decompression in, be volatile oil 144g, extraction yield is 48%, and the extraction ratio of Squalene is 17.76%.
By above-described embodiment parameter and interpretation of result as can be known, among the influence factor to extraction yield in the extracting method of the present invention, extracting pressure〉raw meal particle size〉extraction temperature〉the extraction time.
And the extraction ratio that from other raw materials, extracts Squalene the highest just be 2.09%.The present invention compares with the existing Squalene method of extracting, the extraction ratio height, and technology is simple, and is safe and reliable.
Claims (9)
1. a method of extracting volatile oil from the Lignum Aquilariae Resinatum seed is characterized in that adopting supercritical carbon dioxide to extract.
2. a kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed according to claim 1 is characterized in that may further comprise the steps:
Get dry Lignum Aquilariae Resinatum seed, with pulverizer it is pulverized, place supercritical extraction reactor with the CO of 10-30L/h then
2At 40～50 ℃, extract 1.5～2.5h under 18～25MPa condition, will extract the back fluid then and carry out decompression separation collection extract twice, namely get volatile oil.
3. a kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed according to claim 2 is characterized in that described twice decompression separation comprises first separating still, pressure 5～7MPa, temperature is 45～50 ℃, with second separating still, pressure 5～7MPa, 45～50 ℃ of temperature.
4. a kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed according to claim 2 is characterized in that crossing 20～40 mesh sieves after described Lignum Aquilariae Resinatum seed is pulverized.
5. a kind of method of extracting volatile oil from the Lignum Aquilariae Resinatum seed according to claim 2 is characterized in that water content≤10% of described dry Lignum Aquilariae Resinatum seed.
6. according to each described a kind of method of from the Lignum Aquilariae Resinatum seed, extracting volatile oil among the claim 2-5, it is characterized in that the effective ingredient in the described volatile oil is Squalene.
7. the volatile oil that extracts in the Lignum Aquilariae Resinatum seed according to claim 6 is used for removing the purposes of DPPH free radical.
8. the application preparation of the volatile oil that extracts in the Lignum Aquilariae Resinatum seed according to claim 6 is soft capsule.
9. the application preparation of the volatile oil that extracts in the Lignum Aquilariae Resinatum seed according to claim 8 is characterized in that described soft capsule is mixed with uniform liquid by volatile oil, vitamin E and soybean oil and fills.
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| CN103981020A (en) * | 2014-05-16 | 2014-08-13 | 吴江市英力达塑料包装有限公司 | Extraction-separation method of eaglewood oil |
| CN104940050A (en) * | 2015-05-07 | 2015-09-30 | 孙广娥 | Use of agilawood volatile oil stoste in preparation of skin-care product |
| WO2017084001A1 (en) * | 2015-11-16 | 2017-05-26 | 澄霖农业生技股份有限公司 | Aquilaria food contributing to the cure of diabetic nephropathy in human body |
| CN107778724A (en) * | 2017-11-17 | 2018-03-09 | 广州中医药大学 | A kind of agalloch eaglewood seed extract and its production and use |
| CN113350231A (en) * | 2020-03-04 | 2021-09-07 | 谢慧萍 | Application of agarwood seed extract in preparing skin repairing composition |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981020A (en) * | 2014-05-16 | 2014-08-13 | 吴江市英力达塑料包装有限公司 | Extraction-separation method of eaglewood oil |
| CN104940050A (en) * | 2015-05-07 | 2015-09-30 | 孙广娥 | Use of agilawood volatile oil stoste in preparation of skin-care product |
| WO2017084001A1 (en) * | 2015-11-16 | 2017-05-26 | 澄霖农业生技股份有限公司 | Aquilaria food contributing to the cure of diabetic nephropathy in human body |
| CN108601808A (en) * | 2015-11-16 | 2018-09-28 | 澄霖农业生技股份有限公司 | It is a kind of that human body is contributed to repair the agalloch eaglewood food that diabetes cause renal lesions |
| CN107778724A (en) * | 2017-11-17 | 2018-03-09 | 广州中医药大学 | A kind of agalloch eaglewood seed extract and its production and use |
| CN107778724B (en) * | 2017-11-17 | 2020-04-21 | 广州中医药大学 | Agilawood seed extract and preparation method and application thereof |
| CN113350231A (en) * | 2020-03-04 | 2021-09-07 | 谢慧萍 | Application of agarwood seed extract in preparing skin repairing composition |
| CN113350231B (en) * | 2020-03-04 | 2023-06-02 | 谢慧萍 | Use of agalloch eaglewood seed extract for preparing skin repair composition |
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