CN102688431A - Kidney-reinforcing oral liquid and preparation method thereof - Google Patents
Kidney-reinforcing oral liquid and preparation method thereof Download PDFInfo
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- CN102688431A CN102688431A CN2012101784572A CN201210178457A CN102688431A CN 102688431 A CN102688431 A CN 102688431A CN 2012101784572 A CN2012101784572 A CN 2012101784572A CN 201210178457 A CN201210178457 A CN 201210178457A CN 102688431 A CN102688431 A CN 102688431A
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Abstract
The invention provides a kidney-reinforcing oral liquid, which comprises the following drug components by weight: 10g of cornu cervi parvum, 20g of epimedium, 12g of cassia, 20g of dodder, 30g of wolfberry fruit, 40g of prepared fleece flower root, 20g of fructus psoraleae, 30g of raspberry, 20g of cistanche salsa, 12g of cloves, 40g of rehmanniae, 40g of prepared rehmannia roots, 20g of dogberry, 20g of calcined dragon's bone, 20g of morinda officinalis, 20g of salt semen allii tuberose, 20g of fructus alpiniae oxyphyllae, 12g of white prepared lateral root of aconite, 20g of shizandra, 20g of yam, 12g of Tuckahoe, 12g of alisma, 20g of achyranthes and 20g of eucommia. The drug produced according to the scheme is brownish red to dark brown liquid with sweet smell and semi-sweet semi-pungent taste, has excellent curative effects for reinforcing kidney and supporting yang as well as strengthening tendons and bones, is especially suitable for soreness of the waist and knees, numbness of the muscles and painful joints, intolerance to cold and cold limbs, impotence and the like that are caused by insufficiency of kidney-yang and kidney essence deficiency.
Description
Technical field
The present invention relates to the heath kidney-tonifying field of pharmaceutical technology, refer in particular to the fine and soft osmanthus oral liquor for spleen and kidney of a kind of reinforcing the kidney and supporting YANG, bone and muscle strengthening.
Background technology
The Along with people's growth in the living standard, the man weighs because of work, life, family burden, occurs the renal function heavy losses easily and causes sexual disorder, fatigue and weak, lower limb tiredness; Greenish-yellow complexion, sore waist and aching in the waist and the back, alopecia, poliosis; Tinnitus, deafness, prostatitis, nervous asthma; Male sexual disorder and all kinds of dermatosis in order to alleviate this situation, the medicine of man's health occurs much improving, still on the market; These medicines belong to mostly that take stopgap measures, do not treat to the renal function heavy losses, cause therapeutic effect not obvious or the effect persistency is poor.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of treatment reinforcing the kidney and supporting YANG, bone and muscle strengthening are provided, be particularly useful for the oral liquor for spleen and kidney that soreness of the waist and knees, arthralgia, aversion to cold and cold limbs, sexual impotence due to insufficiency of kidney-YANG, the deficiency of the kidney are used.
For realizing above-mentioned purpose; Technical scheme provided by the present invention is: a kind of oral liquor for spleen and kidney, the medicine component and the weight of said oral liquor for spleen and kidney are: Cornu Cervi Pantotrichum 8～12g, Herba Epimedii 15～25g, Cortex Cinnamomi 10～18g, Semen Cuscutae 15～24g, Fructus Lycii 20～35g, Radix Polygoni Multiflori 35～50g, Fructus Psoraleae 15～23g, Fructus Rubi 25～35g, Herba Cistanches 15～25g, Flos Caryophylli 8～15g, Radix Rehmanniae 30～50g, Radix Rehmanniae Preparata 30～50g, Fructus Corni 15～20g, Os Draconis (calcined) 15～25g, Radix Morindae Officinalis 15～25g, salt Semen Allii Tuberosi 10～20g, Fructus Alpiniae Oxyphyllae 20～30g, gun tab 8～15g, Fructus Schisandrae Chinensis 15～25g, Rhizoma Dioscoreae 10～25g, Poria 8～15g, Rhizoma Alismatis 10～15g, Radix Achyranthis Bidentatae 18～25g, the Cortex Eucommiae 10～25g.
The method for preparing of above-mentioned prescription includes following steps:
First step: press the metering of recipe ingredient and weight ratio;
Second step: Cornu Cervi Pantotrichum is thinly sliced, and adds 10 times of amount 70% ethanol, adopts reflux type to extract secondary, and each 2 hours, merge extractive liquid,, filtration, the A that obtains filtrating is subsequent use;
Third step: with Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae mixing, fragmentation, steam distillation 2～3 hours, it is subsequent use to collect the about 80ml of Aromatic water; Aqueous solution after the distillation is collected in addition;
The 4th step: the medicinal residues of Cornu Cervi Pantotrichum, Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae are mixed with remaining medical material, add 15 times of water gagings and decoct twice, 2 hours for the first time, 1 hour for the second time, collecting decoction, filtration obtained liquor B;
The 5th step: the aqueous solution that third step is obtained adds in the liquor B, and being concentrated into relative density is the clear paste of 1.13 (60 ℃);
The 6th step: the alcohol amount of containing of adding be ethanol 100～150ml of 95% to clear paste, stir, make that to contain the alcohol amount be 50%, left standstill 20～24 hours;
The 7th step: the supernatant of getting the 6th step merges with filtrating A, and decompression recycling ethanol was put cold 20～26 hours;
The 8th step: add refined honey 300g, water adjustment total amount is 880～920ml, and cold preservation 20～24 hours is filtered and obtained liquor C,
The 9th step: Aromatic water adds liquor C, and adjustment total amount to 980～1050ml stirs;
The tenth step: fill, label is pasted in sterilization, and in the carton of packing into, every box-packed 10, in the big carton of packing into, joint sealing promptly gets.
The medicine that said method is made is water preparation, tablet, pill or capsule.
The medicine of this programme production is that brownish red to brown liquid, gas are fragrant, flavor is little sweet, hot; Treatment reinforcing the kidney and supporting YANG, bone and muscle strengthening there are better curative effect, are particularly useful for soreness of the waist and knees, arthralgia, aversion to cold and cold limbs, sexual impotence etc. due to insufficiency of kidney-YANG, the deficiency of the kidney.
The specific embodiment
Be described further in the face of the present invention down, preferred embodiment of the present invention is: the medicine component and the weight of the said oral liquor for spleen and kidney of present embodiment are: Cornu Cervi Pantotrichum 10g, Herba Epimedii 20g, Cortex Cinnamomi 12g, Semen Cuscutae 20g, Fructus Lycii 30g, Radix Polygoni Multiflori Preparata 40g, Fructus Psoraleae 20g, Fructus Rubi 30g, Herba Cistanches 20g, Flos Caryophylli 12g, Radix Rehmanniae 40g, Radix Rehmanniae Preparata 40g, Fructus Corni 20g, Os Draconis (calcined) 20g, Radix Morindae Officinalis 20g, salt Semen Allii Tuberosi 20g, Fructus Alpiniae Oxyphyllae 20g, gun tab 12g, Fructus Schisandrae Chinensis 20g, Rhizoma Dioscoreae 20g, Poria 12g, Rhizoma Alismatis 12g, Radix Achyranthis Bidentatae 20g, Cortex Eucommiae 20g;
The manufacturing approach of said prescription includes following steps:
First step: press the metering of recipe ingredient and weight ratio;
Second step: Cornu Cervi Pantotrichum is thinly sliced, and adds 10 times of amount 70% ethanol, adopts reflux type to extract secondary, and each 2 hours, merge extractive liquid,, filtration, the A that obtains filtrating is subsequent use;
Third step: with Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae mixing, fragmentation, steam distillation 2 hours, it is subsequent use to collect the about 80ml of Aromatic water; Aqueous solution after the distillation is collected in addition;
The 4th step: the medicinal residues of Cornu Cervi Pantotrichum, Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae are mixed with remaining medical material, add 15 times of water gagings and decoct twice, 2 hours for the first time, 1 hour for the second time, collecting decoction, filtration obtained liquor B;
The 5th step: the aqueous solution that third step is obtained adds in the liquor B, and being concentrated into relative density is the clear paste of 1.13 (60 ℃);
The 6th step: the alcohol amount of containing of adding be 95% ethanol 150ml to clear paste, stir, make that to contain the alcohol amount be 50%, left standstill 24 hours;
The 7th step: the supernatant of getting the 6th step merges with filtrating A, and decompression recycling ethanol was put cold 24 hours;
The 8th step: add refined honey 300g, water adjustment total amount is 900ml, and cold preservation 24 hours is filtered and obtained liquor C,
The 9th step: Aromatic water adds liquor C, and the adjustment total amount stirs to 1000ml;
The tenth step: fill, label is pasted in sterilization, and in the carton of packing into, every box-packed 10, in the big carton of packing into, joint sealing promptly gets.
[character] these article are brownish red to brown liquid; Gas is fragrant, and it is little sweet, hot to distinguish the flavor of.
These article 60ml is got in [discriminating] (1), puts in the separatory funnel, and the 30ml that adds diethyl ether, jolting is extracted, and obtains ether solution, volatilizes, and residue adds ethanol 1ml makes dissolving, as need testing solution.Other gets the cinnamic aldehyde reference substance, adds ethanol and processes the solution that every 1ml contains 1 μ l, as reference substance solution.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw need testing solution 5～10 μ l, reference substance solution 2 μ l; Put respectively on same silica gel g thin-layer plate; With petroleum ether (60～90 ℃)-ethyl acetate (85:15) is developing solvent, launches, and takes out; Dry, spray is with dinitrophenylhydrazine ethanol test solution.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(2) get the eugenol reference substance, add ethanol and process the solution that every 1ml contains 20ul, as reference substance solution.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B) test, draw the need testing solution 20ul and the above-mentioned reference substance solution 5ul that differentiate under (1) item, put respectively on same silica gel g thin-layer plate; With petroleum ether (60～90 ℃)-ethyl acetate (9:1) is developing solvent; Launch, take out, dry; Spray is with 5% vanillin sulfuric acid solution, put 105 ℃ dry by the fire to the speckle colour developing clear.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
(3) get these article 50ml, put in the separatory funnel, add ethyl acetate 20ml, jolting is extracted, and obtains ethyl acetate liquid, and evaporate to dryness, residue add ethyl acetate 1ml dissolving, as need testing solution.Other gets psoralen and isopsoralen reference substance, adds ethyl acetate and processes the mixed solution that every 1ml contains 2mg, as reference substance solution.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B); Drawing each 5～10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with petroleum ether (60～90 ℃)-ethyl acetate (20:10); Launch; Take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color.
(4) get these article 50ml, put in the conical flask, add dilute hydrochloric acid 10ml and chloroform 30ml, put in the water-bath and to reflux 2 hours, put coldly, move in the separatory funnel, obtain chloroform solution, evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the emodin reference substance, adds methanol and processes the solution that every 1ml contains 1mg, as reference substance solution.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B); Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with the upper solution of petroleum ether (30～60 ℃)-Ethyl formate-formic acid (15:5:1); Launch; Take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color; Put in the ammonia steam smoked after, it is red that speckle shows.
(5) get these article 50ml, put in the separatory funnel, add the ethyl acetate jolting and extract 2 times, each 20ml merges ethyl acetate liquid, and evaporate to dryness, residue add ester acetoacetic ester 1ml makes dissolving, as need testing solution.Other gets Fructus Lycii control medicinal material 0.5g, and decocte with water 30 minutes is put coldly, filters, and filtrating is shone medical material solution in pairs with legal system.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 B); Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with ethyl acetate-chloroform-formic acid (3:6:1); Launch; Take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
[inspection] aconitine limit precision is measured these article 100ml, puts in the conical flask, adds ammonia solution 10ml, jolting 10 minutes; Placed 2 hours, the 100ml that adds diethyl ether, jolting 1 hour was placed 24 hours; Obtain ether solution, evaporate to dryness, residue add dehydrated alcohol makes dissolving, moves in the 2ml measuring bottle; Add dehydrated alcohol and be diluted to scale, shake up, as need testing solution.Other gets the negative sample 100ml that lacks Radix Aconiti Lateralis Preparata, processes negative controls with method.Precision takes by weighing aconitine reference substance (Nat'l Pharmaceutical & Biological Products Control Institute, lot number: 110720-200410), add dehydrated alcohol and process the solution that every 1ml contains 2mg, as reference substance solution again.According to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test; Drawing each 20 μ l of above-mentioned need testing solution and negative controls, reference substance solution 1 μ l, put respectively on same silica gel g thin-layer plate, is developing solvent with methylene chloride-methanol-ammonia (20:4:0.2); Launch; Take out, dry, spray is with the improvement bismuth potassium iodide test solution.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on the speckle that occurs should or speckle appear less than the speckle of reference substance.
Relative density should be not less than 1.06 (an appendix VII of Chinese Pharmacopoeia version in 2000 A).
PH value should be 4.0～6.0 (an appendix VII of Chinese Pharmacopoeia version in 2000 G).
Other should meet each item regulation (an appendix I of Chinese Pharmacopoeia version in 2000 J) relevant under the mixture item.
[assay] measured according to HPLC (an appendix VI of Chinese Pharmacopoeia version in 2000 D).
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; Methanol-water (55:45) is a mobile phase; Detect wavelength 270nm.Number of theoretical plate should be not less than 1000 by the icariin peak.
The preparation of reference substance solution: it is an amount of that precision takes by weighing the icariin reference substance, adds methanol and process the solution that every 1ml contains 30 μ g, promptly gets.
The preparation of need testing solution: precision is measured these article 20ml, puts in the conical flask, adds ethyl acetate 25ml; Supersound process 15 minutes leaves standstill, and obtains vinegar ester ethyl ester extracting solution; Repeat aforesaid operations 5 times, merge ethyl acetate liquid, evaporate to dryness; Residue adds mobile mutual-assistance dissolving and moves in the 25ml measuring bottle, adds mobile phase and is diluted to scale, as need testing solution.
Algoscopy: accurate respectively test sample and each 10 μ l of reference substance solution of drawing, inject high performance liquid chromatograph, measure, promptly get.
Every of these article contain icariin (C
33H
40O
15) meter, must not be less than 0.20mg
[function with cure mainly] reinforcing the kidney and supporting YANG, bone and muscle strengthening.Be applicable to diseases such as soreness of the waist and knees due to insufficiency of kidney-YANG, the deficiency of the kidney, arthralgia, aversion to cold and cold limbs, sexual impotence.
[usage and consumption] is oral, and a 10ml 2 times on the one, obeys sooner or later on an empty stomach.
Shady and cool dry place is put in [storage] sealing.
The embodiment of the above only is the present invention's preferred embodiment, is not to limit practical range of the present invention with this, so the variation that all shapes according to the present invention, principle are done all should be encompassed in protection scope of the present invention.
Claims (3)
1. oral liquor for spleen and kidney, it is characterized in that: the medicine component and the weight of said oral liquor for spleen and kidney are: Cornu Cervi Pantotrichum 8～12g, Herba Epimedii 15～25g, Cortex Cinnamomi 10～18g, Semen Cuscutae 15～24g, Fructus Lycii 20～35g, Radix Polygoni Multiflori 35～50g, Fructus Psoraleae 15～23g, Fructus Rubi 25～35g, Herba Cistanches 15～25g, Flos Caryophylli 8～15g, Radix Rehmanniae 30～50g, Radix Rehmanniae Preparata 30～50g, Fructus Corni 15～20g, Os Draconis (calcined) 15～25g, Radix Morindae Officinalis 15～25g, salt Semen Allii Tuberosi 10～20g, Fructus Alpiniae Oxyphyllae 20～30g, gun tab 8～15g, Fructus Schisandrae Chinensis 15～25g, Rhizoma Dioscoreae 10～25g, Poria 8～15g, Rhizoma Alismatis 10～15g, Radix Achyranthis Bidentatae 18～25g, the Cortex Eucommiae 10～25g.
2. method for preparing that is used for the said prescription of claim 1, it is characterized in that: said method for preparing includes following steps:
First step: press the metering of recipe ingredient and weight ratio;
Second step: Cornu Cervi Pantotrichum is thinly sliced, and adds 10 times of amount 70% ethanol, adopts reflux type to extract secondary, and each 2 hours, merge extractive liquid,, filtration, the A that obtains filtrating is subsequent use;
Third step: with Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae mixing, fragmentation, steam distillation 2～3 hours, it is subsequent use to collect the about 80ml of Aromatic water; Aqueous solution after the distillation is collected in addition;
The 4th step: the medicinal residues of Cornu Cervi Pantotrichum, Cortex Cinnamomi, Flos Caryophylli, Fructus Alpiniae Oxyphyllae are mixed with remaining medical material, add 15 times of water gagings and decoct twice, 2 hours for the first time, 1 hour for the second time, collecting decoction, filtration obtained liquor B;
The 5th step: the aqueous solution that third step is obtained adds in the liquor B, and being concentrated into relative density is the clear paste of 1.13 (60 ℃);
The 6th step: the alcohol amount of containing of adding be 95% ethanol 100-150ml to clear paste, stir, make that to contain the alcohol amount be 50%, left standstill 20～24 hours;
The 7th step: the supernatant of getting the 6th step merges with filtrating A, and decompression recycling ethanol was put cold 20～26 hours;
The 8th step: add refined honey 300g, water adjustment total amount is 880～920ml, and cold preservation 20～24 hours is filtered and obtained liquor C,
The 9th step: Aromatic water adds liquor C, and adjustment total amount to 980～1050ml stirs;
The tenth step: fill, label is pasted in sterilization, and in the carton of packing into, every box-packed 10, in the big carton of packing into, joint sealing promptly gets.
3. method for preparing according to claim 2 is characterized in that: the medicine of making is water preparation, tablet, pill or capsule.
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Cited By (13)
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| CN102988703A (en) * | 2012-12-27 | 2013-03-27 | 青岛华仁信息技术开发有限公司 | Traditional Chinese medicine preparation for treating kidney-yang deficiency and preparation method thereof |
| CN102988728A (en) * | 2012-10-29 | 2013-03-27 | 严中明 | Chinese medicinal formulae for treating kidney deficiency type lumbar muscle degeneration |
| CN103330898A (en) * | 2013-07-18 | 2013-10-02 | 黄石市中心医院（普爱院区） | Traditional Chinese medicine composition for curing impotence and prospermia, as well as preparation method and application of traditional Chinese medicine composition |
| CN103341107A (en) * | 2013-06-27 | 2013-10-09 | 柳晖 | Chinese medicine composition for treating enuresis |
| CN103977215A (en) * | 2014-05-27 | 2014-08-13 | 张耀华 | Traditional Chinese medicine for treating deficiency of kidneys |
| CN104161951A (en) * | 2014-08-18 | 2014-11-26 | 河南科技大学第一附属医院 | Nutritional bone-strengthening powder and preparation method thereof |
| CN105125809A (en) * | 2015-08-31 | 2015-12-09 | 董中立 | Healthy beverage and preparation method thereof |
| CN105288093A (en) * | 2015-12-05 | 2016-02-03 | 徐萍 | Medicine for treating impotence and preparation method thereof |
| CN105456382A (en) * | 2016-01-15 | 2016-04-06 | 白云兵 | Kidney tonifying medicine |
| CN105770317A (en) * | 2016-03-14 | 2016-07-20 | 李光亮 | Wine with effects of tonifying kidneys and strengthening bones and method for preparing wine |
| CN106511877A (en) * | 2016-11-15 | 2017-03-22 | 臧明礼 | Pure traditional Chinese medicinal preparation for treating human kidney deficiency |
| CN107213335A (en) * | 2017-06-13 | 2017-09-29 | 史铜川 | A kind of tonifying kidney and strengthening yang medicine and preparation method |
| CN112933203A (en) * | 2020-11-26 | 2021-06-11 | 四川烨哼健康药房有限公司 | Traditional Chinese medicine composition of medicinal liquor for resisting fatigue and improving sexual function and preparation method of traditional Chinese medicine composition |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988728A (en) * | 2012-10-29 | 2013-03-27 | 严中明 | Chinese medicinal formulae for treating kidney deficiency type lumbar muscle degeneration |
| CN102988703A (en) * | 2012-12-27 | 2013-03-27 | 青岛华仁信息技术开发有限公司 | Traditional Chinese medicine preparation for treating kidney-yang deficiency and preparation method thereof |
| CN102988703B (en) * | 2012-12-27 | 2014-09-03 | 青岛华仁技术孵化器有限公司 | Traditional Chinese medicine preparation for treating kidney-yang deficiency and preparation method thereof |
| CN103341107A (en) * | 2013-06-27 | 2013-10-09 | 柳晖 | Chinese medicine composition for treating enuresis |
| CN103330898A (en) * | 2013-07-18 | 2013-10-02 | 黄石市中心医院（普爱院区） | Traditional Chinese medicine composition for curing impotence and prospermia, as well as preparation method and application of traditional Chinese medicine composition |
| CN103977215A (en) * | 2014-05-27 | 2014-08-13 | 张耀华 | Traditional Chinese medicine for treating deficiency of kidneys |
| CN104161951A (en) * | 2014-08-18 | 2014-11-26 | 河南科技大学第一附属医院 | Nutritional bone-strengthening powder and preparation method thereof |
| CN105125809A (en) * | 2015-08-31 | 2015-12-09 | 董中立 | Healthy beverage and preparation method thereof |
| CN105288093A (en) * | 2015-12-05 | 2016-02-03 | 徐萍 | Medicine for treating impotence and preparation method thereof |
| CN105456382A (en) * | 2016-01-15 | 2016-04-06 | 白云兵 | Kidney tonifying medicine |
| CN105770317A (en) * | 2016-03-14 | 2016-07-20 | 李光亮 | Wine with effects of tonifying kidneys and strengthening bones and method for preparing wine |
| CN106511877A (en) * | 2016-11-15 | 2017-03-22 | 臧明礼 | Pure traditional Chinese medicinal preparation for treating human kidney deficiency |
| CN107213335A (en) * | 2017-06-13 | 2017-09-29 | 史铜川 | A kind of tonifying kidney and strengthening yang medicine and preparation method |
| CN112933203A (en) * | 2020-11-26 | 2021-06-11 | 四川烨哼健康药房有限公司 | Traditional Chinese medicine composition of medicinal liquor for resisting fatigue and improving sexual function and preparation method of traditional Chinese medicine composition |
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Application publication date: 20120926 |