CN101002809A - Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof - Google Patents
Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof Download PDFInfo
- Publication number
- CN101002809A CN101002809A CN 200710051331 CN200710051331A CN101002809A CN 101002809 A CN101002809 A CN 101002809A CN 200710051331 CN200710051331 CN 200710051331 CN 200710051331 A CN200710051331 A CN 200710051331A CN 101002809 A CN101002809 A CN 101002809A
- Authority
- CN
- China
- Prior art keywords
- jin
- sha
- pternopetali vulgaris
- radix pternopetali
- extractive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A medicine containing the extract of pternoptalum root for treating stomach-ache, abdominalgia, peptic ulcer, smooth muscle spasm, etc is prepared from pternoptalum root through extracting in alcohol or water and preparing tablets or capsules.
Description
Technical field
The present invention relates to a kind of preparation method that contains the medicine of extractive of Zi-Jin-Sha, the invention still further relates to the medicine that contains extractive of Zi-Jin-Sha at analgesia, antiulcer and separate application in the spasm medicine.
Background technology
Radix Pternopetali vulgaris is Tujia's common drug, is distributed in Hubei, Hunan, Chongqing etc. and economizes ground.Effect with warming spleen and stomach for dispelling cold, regulating QI to relieve pain.Be used for the treatment of stomachache, stomachache, chest and hypochondrium, rheumatic arthritis, traumatic injury, venom etc.Because the effect brilliance enjoys high reputation on Shennongjia, Yichang and other places, finds a good sale at home and abroad.Document has different records to the Ji Yuan of Radix Pternopetali vulgaris, and " national Chinese herbal medicine compilation ", " Chinese medicine voluminous dictionary " record Radix Pternopetali vulgaris is the root of Umbelliferae capsule lobe celery plant capsule lobe celery Pternopetalum vulgare (Dunn) Hand.--Mazz.." Hubei flora " record Radix Pternopetali vulgaris turns the root of celery Angelica polymorpha Maxim. for the Umbelliferae archangel." Chinese Shennongjia natural resources of Chinese medicinal materials " record Radix Pternopetali vulgaris has another name called Rhizoma Hedychii Coronarii, is the root of Umbelliferae capsule lobe apium plant capsule lobe celery Pternopetalum vulgare (Dunn) Hand.--Mazz.; Or the root of umbelliferae angelica platymiscium Bletilla striata (Thunb.ex A.Murray)Rchb.f. Radix Angelicae Pubescentis Angelica polymorpha Maxim..Utilize the water extract of Radix Pternopetali vulgaris or ethanol extract to be prepared into medicine and do not see relevant report.
Summary of the invention
The objective of the invention is to provide a kind of and contain the process for preparing medicine of extractive of Zi-Jin-Sha and at analgesia, antiulcer and separate the spasm medicinal application.
The object of the present invention is achieved like this:
A kind of process for preparing medicine that contains extractive of Zi-Jin-Sha,
Radix Pternopetali vulgaris water extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add entry, soaked 1 hour, and connected extractor, twice of reflux, extract,, each 1.5-3 hour, obtain Radix Pternopetali vulgaris volatile oil in oil water separator, water liquid filters, and filtrate concentrating obtains extractum, constant pressure and dry or vacuum drying get the Radix Pternopetali vulgaris water extract;
Radix Pternopetali vulgaris alcohol extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add 95% ethanol, soaked 1 hour, connect extractor, reflux, extract, twice each 1.5-3 hour, is filtered, seal behind the filtrate decompression recovered alcohol, room temperature is placed, and separates the upper strata oily liquids, lower floor's extractum vacuum drying, with oily liquids mixing (adding right amount of auxiliary materials such as starch in case of necessity), get the Radix Pternopetali vulgaris alcohol extract.
Get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, and be equivalent to crude drug in whole 10g for a, get mixture 1000-10000 part, add tabletting behind the adjuvant, making every, to contain chemical compound (sweet) bisabolangelone be 0-30mg, β-phellandrene 0-3mg obtains tablet medicine;
Get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, and be equivalent to crude drug in whole 10g for a, mix, get mixture 1000-10000 part, add adjuvant, granulate, granulate, encapsulated, make that to contain chemical compound (sweet) bisabolangelone in every capsules be 0-30mg, β-phellandrene 0-3mg.
Used adjuvant can be one or more in tablet such as microcrystalline Cellulose, modified starch, magnesium stearate, ethyl cellulose, methylcellulose, hydroxyethyl-cellulose, hydroxypropyl emthylcellulose, sodium carboxymethyl cellulose and the capsule adjuvant commonly used.
The application of extractive of Zi-Jin-Sha in analgesic.
The application of extractive of Zi-Jin-Sha in anti-ulcer medicament.
The application of extractive of Zi-Jin-Sha in the spasmolytic medicine.
By the invention provides the medicine that contains extractive of Zi-Jin-Sha that method obtains, extractive of Zi-Jin-Sha is made tablet or capsule, taking convenience; The made medicine that contains extractive of Zi-Jin-Sha has made full use of the active ingredient of Radix Pternopetali vulgaris, has analgesia, antiulcer and spasmolysis, and stomachache, stomachache, peptic ulcer, gastrointestinal smooth muscular spasm, bronchial smooth muscle are had better curative effect.
Description of drawings
Fig. 1 is that Radix Pternopetali vulgaris volatile oil of the present invention carries out GC-MS analysis peak figure.
Fig. 2 is the thin-layer chromatogram of The compounds of this invention 1 and 2.
Fig. 3 is the high-efficient liquid phase chromatogram of chemical compound 1.
Fig. 4 is the ultra-violet analysis spectrogram of chemical compound 1.
Fig. 5 is the high-efficient liquid phase chromatogram of chemical compound 2.。
Fig. 6 is the ultra-violet analysis spectrogram of chemical compound 2.
Fig. 7 is the high performance liquid chromatography of extract.
The specific embodiment
The preparation method of the medicine that the present invention relates to is as follows:
One, raw material: Radix Pternopetali vulgaris; Pick up from Shennongjiawooded Area wooden fish town.Turn the root of celery Angelica polymorpha Maxim. through being accredited as the umbelliferae angelica platymiscium.
Two, the pharmacognosy feature of Radix Pternopetali vulgaris plant
1, former plant trait
Perennial herb, high 40 ~ 90 ~ 160cm, gas is hot fragrant.Stem is upright, hollow, and green or band purple, branch is arranged at top.The leaf alternate, basal leaf is early withered, 2 ~ 3 times winglike compound leaves of stem leaf, lobule is avette to ovum shape lanceolar, long 3 ~ 7cm, wide 1 ~ 3cm, tip be point or tail point gradually, and leaflet edge has irregular thick sharp sawtooth, along vein undercoat is arranged.Petiole is long, and base portion is the sheath shape and embraces stem.Give birth on the universal umbel top, and phyllary does not exist usually, and total bennet, umbrella obstruct all close by short rough hair, bennet 20 ~ 30, and petal 5, white, cochlear are to obovate, and stamen 5 is longer than petal, and style 2 is elongated.Bloom 7 ~ August, 8 ~ October the result.Diachenium is flat, and is oval to wide ellipse, long 5 ~ 7mm, wide 3 ~ 5mm.Grow in the mountain valley limes marginis or roadside weeds clump of height above sea level 1300 ~ 2000m.The thick shape of root, meat, cylindric.
2, medical material character
Root is closely cylindrical, long 10～25cm, and many supporting roots are arranged at the bottom.Root head class sphere, diameter 1.5～5cm.The surface Huang exhausts color to brownish black, the most vertical wrinkles of tool and the hole skin of growing crosswise.There is purple to exhaust color or yellow green stem, leaf residue.Main root twists more, and the vertical wrinkle in surface is intensive, vertical collude darker; Surplus the supporting root 3 to 10 piece, upper coarse and lower fine, how crooked.Matter is harder, little lignify.The section yellow-white has the crack to yellowish-brown, and cambium layer is obvious, and there is brown oil drop in skin zone, is extruded with brown oil and oozes out.Gas perfume (or spice) is special, and Xin Xiang is refrigerant.Sweet in the mouth, pungent, little hardship.
Three, the Radix Pternopetali vulgaris volatile oil component is analyzed
3.1, the volatile oil component analysis
3.1.1 volatile oil extracts
Cut essence after getting the Radix Pternopetali vulgaris weighing.Place the 1000ml round-bottomed flask, add suitable quantity of water, soaked 1 hour.Connect condensation reflux unit and volatile oil determination apparatus, place temperature adjustable electrically heated putting, extracted 4 hours, get volatile oil, oil yield is 0.5%.
3.1.2 volatile oil testing conditions
The gas chromatography-mass spectrum condition: instrument is the U.S. HP68590GC/5973MS of an Agilent Technologies company gas chromatograph-mass spectrometer.GC condition: HP-5MS quartz capillary (30m * 0.25mm * 0.25 μ m); 80 ~ 240 ℃ of column temperatures, 3 ℃/min of temperature programming; 250 ℃ of injector temperatures; Press 100kPa before the post; Sample size 0.06 μ l; Split ratio 15:1; Carrier gas is a high-pure helium.MS condition: ionization mode EI; Electron energy 70eV; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; Mass range 35 ~ 450; The qualitative back of wikey7n.1 library searching is confirmed.
3.1.3 volatile oil testing result
By above-mentioned condition Radix Pternopetali vulgaris volatile oil is carried out GC-MS and analyze, isolate 69 peaks.See Fig. 1, related data is manually resolved and is checked in machine examination rope NIST02 standard mass spectrometric data storehouse as calculated, has confirmed wherein 54 compositions.Calculate the relative amount of each composition in volatile oil with the peak area normalization method, the results are shown in Table 1.
The chemical constituent of table 1 Radix Pternopetali vulgaris volatile oil and relative percentage composition
| Sequence number | Chemical compound | Percentage composition (%) |
| 1 | α-thujene | 0.10 |
| 2 | Australene | 11.86 |
| 3 | Camphene | 0.10 |
| 4 | Nopinene | 0.54 |
| 5 | Myrcene | 1.97 |
| 6 | 2-methyl-2 penetenoic acid | 0.10 |
| 7 | α-phellandrene | 13.35 |
| 8 | The 3-carene | 1.72 |
| 9 | α-terpinene | 0.36 |
| 10 | Cymol | 3.48 |
| 11 | β-phellandrene | 23.91 |
| 12 | Ocimene | 0.05 |
| 13 | γ-Song Youxi | 0.05 |
| 14 | The 4-methylphenol | 2.60 |
| 15 | Terpinolene | 2.60 |
| 16 | The 6-camphenone | 0.28 |
| 17 | 3 Methylbutanoic acid-3-methyl butyl ester | 2.72 |
| 18 | Suitable-rose oxide | 0.10 |
| 19 | 1-methyl-4-isopropyl-2-cyclohexene-1-alcohol | 1.01 |
| 20 | Instead-Flos Rosae Rugosae alkene | 0.08 |
| 21 | Oleum Pini-1-alcohol | 0.77 |
| 22 | 2,5-diethyl thiophene | 6.34 |
| 23 | 4-isopropyl-2-cyclohexene-1-ketone | 0.89 |
| 24 | Suitable-piperitol | 0.47 |
| 25 | The own ester of 3 Methylbutanoic acid | 0.39 |
| 26 | 10-Pinen-3-ol | 0.34 |
| 27 | Instead-piperitol | 0.49 |
| 28 | Carvacrol methyl ether | 0.10 |
| 29 | N-hexyl valerate | 0.27 |
| 30 | Piperitone | 0.05 |
| 31 | 4-hydroxy-3-methyl 1-Phenylethanone. | 1.87 |
| 32 | Borneol acetate | 0.94 |
| 33 | Acetic acid lavandula angustifolia ester | 0.43 |
| 34 | The 2-Methyl Butyric Acid heptyl ester | 0.30 |
| 35 | Heptyl valerate | 0.42 |
| 36 | 4-ethyl-1, the 2-dimethoxy benzene | 0.05 |
| 37 | Citronellyl acetate | 0.10 |
| 38 | δ-elemene | 0.84 |
| 39 | The valeric acid monooctyl ester | 0.28 |
| 40 | Ene | 0.21 |
| 41 | α-Gu Yunxi | 0.39 |
| 42 | β-farnesene | 0.57 |
| 43 | Fragrance-curcumene | 0.22 |
| 44 | α-farnesene | 0.84 |
| 45 | β-bergaptene | 0.60 |
| 46 | β-bisabolene | 0.32 |
| 47 | δ-cadinene | 0.25 |
| 48 | α-bisabolene | 0.38 |
| 49 | Nerolidol | 0.05 |
| 50 | Citronellyl valerate | 0.23 |
| 51 | Spathulenol | 0.32 |
| 52 | Globulol | 0.78 |
| 53 | α-bisabolol | 1.67 |
| 54 | 9-15 carbene alcohol | 0.87 |
3.2 composition extracts and analyzes
3.2.1, the preparation of laboratory sample
Take by weighing the 1.0kg Radix Pternopetali vulgaris, extract 4 times with ethyl acetate backflow, each 1.5h, merge extractive liquid, obtains ethyl acetate extract behind the concentrating under reduced pressure, use for separating.
3.2.2, column chromatography for separation
Get 200g silica gel (200-300 order) wet method dress post.Get sample on the ethyl acetate extract dry method.With petroleum ether-ethyl acetate (4: 1) normal pressure eluting, every 50ml is a flow point, obtains 46 flow points altogether.Detect through TLC, merge identical flow point, recrystallization obtains chemical compound 1 and 2 at flow point 6～9 and 11～15 respectively.Chemical compound 1 is a white needle-like crystals, and chemical compound 2 is the white plates crystal.
With these two kinds of chemical combination object points on same block of silica gel plate, with petroleum ether: ethyl acetate=4: 1 is done developing solvent.Obtain two single speckles respectively, their Rf value is respectively R
F1=0.54, R
F2=0.27, as Fig. 2.Detect under ultraviolet light, under the 254nm, two chemical compounds all have purple fluorescence; Under the 365nm, chemical compound 1 has yellow-green fluorescence, and chemical compound 2 no fluorescence.
3.2.3, high-efficient liquid phase chromatogram condition
Chromatographic column is YMC-Pack AQ C18,250 * 4.6mm I.D; Mobile phase is acetonitrile-water (70: 30); Sample size is 10 μ l, and flow velocity is 1.0ml/min; Column temperature is 25 ℃, and the detection wavelength is 254nm.
3.2.4, compound structure identifies
Through being with document control compound 1 structure:
C16H14O4 270.28
Isoimperatorin (Isoimperatorin)
The structure of chemical compound 2 is:
(sweet) bisabolangelone (Bisabolangelone)
3.2.5, the high performance liquid chromatography of extract
Prepare for test agent: take by weighing the 2.0kg Radix Pternopetali vulgaris, add methanol 20ml and soak 1.5h, backflow 0.5h, 0.45 μ membrane filtration is used for measuring.
Chromatographic condition: chromatographic column is YMC-PackAQ C18,250 * 4.6mm I.D; Mobile phase is acetonitrile-water (70: 30); Sample size is 10 μ 1, and flow velocity is 1.0ml/min; Column temperature is 25 ℃, and the detection wavelength is 254nm.Chemical compound 1 retention time 9.15 ± 0.5min wherein, chemical compound 2 retention times 5.03 ± 0.5min
Four, preparation method
1, Radix Pternopetali vulgaris water extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add entry, soaked 1 hour, and connected extractor, twice of reflux, extract,, each 1.5-3 hour, obtain Radix Pternopetali vulgaris volatile oil in oil water separator, water liquid filters, and filtrate concentrating obtains extractum, constant pressure and dry or vacuum drying get the Radix Pternopetali vulgaris water extract.
2, Radix Pternopetali vulgaris alcohol extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add 95% ethanol, soaked 1 hour, connect extractor, reflux, extract, twice each 1.5-3 hour, is filtered, seal behind the filtrate decompression recovered alcohol, room temperature is placed, and separates the upper strata oily liquids, lower floor's extractum vacuum drying, with oily liquids mixing (adding right amount of auxiliary materials such as starch in case of necessity), get the Radix Pternopetali vulgaris alcohol extract.
3, contain the preparation of Radix Pternopetali vulgaris medicinal tablet medicine: get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, 0～10 part of Radix Pternopetali vulgaris oil, and be equivalent to crude drug in whole 10g for a, get mixture 1000-10000 part, tabletting behind the adding adjuvant, making every, to contain chemical compound (sweet) bisabolangelone be 0-30mg, and β-phellandrene 0-3mg obtains tablet medicine.
4, contain the preparation of Radix Pternopetali vulgaris medicine capsule: get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, 0～10 part of Radix Pternopetali vulgaris oil, and be equivalent to crude drug in whole 10g for a, mix, get mixture 1000-10000 part, add adjuvant, granulate granulate, encapsulated, make that to contain chemical compound (sweet) bisabolangelone in every capsules be 0-30mg, β-phellandrene 0-3mg.
Used adjuvant can be one or more in tablet such as microcrystalline Cellulose, modified starch, magnesium stearate, ethyl cellulose, methylcellulose, hydroxyethyl-cellulose, hydroxypropyl emthylcellulose, sodium carboxymethyl cellulose and the capsule adjuvant commonly used.
Five, pharmacological action
5.1, medication preparation
Get about 100g Radix Pternopetali vulgaris, weighing divides three extractions.Place the 500ml round-bottomed flask, add suitable quantity of water, soaked 1 hour.Connect condensation reflux unit and volatile oil extractor, place temperature adjustable electrically heated putting, 50V extracted 4 hours down, obtained volatile oil.Continue decocting half an hour again, filter, filtrate concentrates, and obtains extractum.It is dry down to be put in 80 ℃ of thermostatic drying chambers, promptly gets water solubility extract.
5.2, the application of extractive of Zi-Jin-Sha in analgesic.
Get female kunming mice and place (55 ± 0.5 ℃) on the hot plate dolorimeter, measure mice from being placed on the hot plate to the metapedes required time occurring licking, less than 10 seconds or give it up greater than 60 seconds or leaper.Repeat to survey its normal pain threshold 2 times, get two subnormal threshold of pain meansigma methodss as this Mus administration before the basic threshold of pain.60 mices are divided into 6 groups at random by medicine preceding threshold of pain equilibrium.Every group gavages different pharmaceutical respectively, and administration 3 days is in not measuring the pain threshold in 30min, 60min, the 120min after the administration after time administration.The results are shown in Table 2.
Table 2 extractive of Zi-Jin-Sha to the analgesic activity of mice thermostimulation pain (
N=10)
| Group | Dosage (mg/kg) | 30min pain threshold raising rate (%) | 60min pain threshold raising rate (%) | 120min pain threshold raising rate (%) |
| Normal saline tramadol hydrochloride water extract low dosage water extract high dose volatile oil low dosage volatile oil high dose | / 16.0 415.0 830.0 8.0 16.0 | 1.60±12.65 59.89±19.12** 31.33±18.67 6.81±28.36 35.27±5.71** 60.42±36.98** | -1.54±11.13 64.29±16.92** 31.7±21.84** 4.71±1.88 37.16±15.18* 70.18±8.70** | -2.83±9.51 74.20±15.10** 90.78±33.50** 99.68±28.31** 40.15±15.25* 83.64±23.41** |
Compare * p＜0.05, * * p＜0.01 with the normal saline group
Get 60 of kunming mices, be divided into 6 groups at random.Every group gavages different pharmaceutical respectively, administration 3 days, after time administration behind the 30min each Mus lumbar injection 0.8% acetum (0.2ml/ only) observe and record injection acetic acid after the number of times of writhing response appears occurring in time (latent time) of writhing response and the 10-20min for the first time.The results are shown in Table 3.
Table 3 extractive of Zi-Jin-Sha Dichlorodiphenyl Acetate induced mice turn round body analgesic activity (x ± s, n=10)
| Group | Dosage (mg/kg) | Incubation period (s) | Turn round body number of times (inferior) |
| Normal saline tramadol hydrochloride water extract low dosage water extract high dose volatile oil low dosage volatile oil high dose | / 16.0 415.0 830.0 8.0 16.0 | 230.75±60.04 408.75±124.13** 261.29±61.21 344.17±159.69* 290.86±62.70* 396.60±74.47** | 19.1±7.4 15.4±4.1** 16.7±6.4** 10.3±4.5** 8.3±4.1** 11.3±4.0** |
Compare * p＜0.05, * * p＜0.01 with the normal saline group
The result shows that Radix Pternopetali vulgaris water extract, volatile oil hit caused pain to physics and chemistry thorn and all have significant analgesia role.
5.2. the application of extractive of Zi-Jin-Sha in anti-ulcer medicament.
Get 60 of kunming mices, be divided into 6 groups at random.Every group gavages different pharmaceutical respectively, fasting 24h before not inferior administration.Do not press 100mg/kg with aspirin-alcoholic solution of 2mmol/l after time administration and irritate stomach.Behind the 4h, mice is put to death, get stomach, find out ulcer surface and observe, with the ulcer expression ulcer index of counting with anatomic microscope.
The effect of table 4 extractive of Zi-Jin-Sha anti-gastric-ulcer
[19](x ± s, n=10)
| Group | Dosage (mg/kg) | Ulcer is counted | The OD value |
| Normal saline cimetidine water extract low dosage water extract high dose volatile oil low dosage volatile oil high dose | / 66.0 415.0 830.0 8.0 16.0 | 28.0±3.3 3.8±1.2** 7.8±1.4** 2.0±3.3** 3.3±2.1** 1.7±1.6** | 2.240±0.353 2.550±0.217 4.936±0.4721** 3.294±0.568* 3.330±0.732* 3.122±0.531 |
Compare * * p＜0.01 with the normal saline group
The result shows that Radix Pternopetali vulgaris water extract, volatile oil have tangible antiulcer action to aspirin-alcoholic solution induced mice gastric ulcer.Radix Pternopetali vulgaris water extract and volatile oil can promote gastric tissue PGE
2Generation, play the protection gastric mucosa effect.
5.3, the application of extractive of Zi-Jin-Sha in separating the spasm medicine.
Influence to the intestinal smooth muscle motion
Get 24 hours rabbit of fasting, the stripped ileum flesh specimen of preparation, the long 2cm of specimen connects tonotransducer with specimen, places in the thermostatic bath that fills tyrode's solution (37 ± 0.5 ℃), continuously aerating oxygen.Record myenteron shrinkage curve.Balance 30min, treat baseline stability after, drip the Radix Pternopetali vulgaris volatile oil of various dose, muscular tension curve after the record administration is observed Radix Pternopetali vulgaris volatile oil to the exsomatize effect of myenteron of rabbit.The results are shown in Table 5.
The influence that table 5 extractive of Zi-Jin-Sha moves to intestinal smooth muscle (
N=6)
| Group | Dosage (ug/ml) | Tension force (g) before the administration | Tension force after the administration (g) | Suppression ratio (%) |
| The water extract low dosage | 200 | 3.21±0.27 | 2.30±0.12* | 28.12 |
| The water extract high dose | 400 | 3.83±0.21 | 2.10±0.23* | 45.15 |
| Volatile oil low dosage volatile oil high doses of |
20 40 50 50 | 3.71±0.22 3.83±0.37 3.94±0.32 3.36±0.28 | 2.56±0.19* 2.10±0.20* 2.94±0.26* 1.68±0.17* | 31.00 45.20 25.76 50.00 |
With compare * p＜0.05 before the administration
Get 50 of Kunming mouses about 18-22g, be divided into 5 groups at random.Every group gavaged relative medicine respectively 3 days, and every day 1 time, fasting is 12 hours before the experiment, freely drinks water.Test and be administered once again in preceding 30 minutes, after 30 minutes, the charcoal end liquid of preparation is newly irritated stomach, irritate stomach and take off neck after 15 minutes and put to death, calculate charcoal end propelling rate
[24](charcoal end glue propelling rate (%)=(charcoal end glue is at the enteral advance distance)/(small intestinal total length) * 100%).The results are shown in Table 6.
The influence that table 6 extractive of Zi-Jin-Sha moves to mouse small intestine (x ± s, n=10)
| Group | Dosage (mg/kg) | Propelling rate (%) |
| The normal saline group | / | 54.39±4.61 |
| The water extract low dosage | 415.0 | 21.96±2.47** |
| The water extract high dose | 830.0 | 20.44±9.27** |
| The volatile oil low dosage | 8.0 | 25.23±6.27** |
| The volatile oil high dose | 16.0 | 23.57±5.71** |
Compare * p＜0.05, * * p＜0.01 with the normal saline group
The result shows that extractive of Zi-Jin-Sha has the effect that suppresses the intestinal smooth muscle motion.Chemical compound 2 is one of its main effective ingredient.
Claims (9)
1, a kind of process for preparing medicine that contains extractive of Zi-Jin-Sha is characterized in that:
Radix Pternopetali vulgaris water extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add entry, soaked 1 hour, and connected extractor, twice of reflux, extract,, each 1.5-3 hour, obtain Radix Pternopetali vulgaris volatile oil in oil water separator, water liquid filters, and filtrate being concentrated into obtains extractum, constant pressure and dry or vacuum drying get the Radix Pternopetali vulgaris water extract.
2, a kind of process for preparing medicine that contains extractive of Zi-Jin-Sha is characterized in that: Radix Pternopetali vulgaris alcohol extract preparation: get the Radix Pternopetali vulgaris decoction pieces, add 95% ethanol, soaked 1 hour, and connected extractor, twice of reflux, extract,, each 1.5-3 hour, filter, seal behind the filtrate decompression recovered alcohol, room temperature is placed, separate the upper strata oily liquids, lower floor's extractum vacuum drying with oily liquids mixing (adding right amount of auxiliary materials such as starch in case of necessity), gets the Radix Pternopetali vulgaris alcohol extract.
3, the process for preparing medicine that contains extractive of Zi-Jin-Sha according to claim 1 and 2 is characterized in that: get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, be equivalent to crude drug in whole 10g for a, get mixture 1000-10000 part, tabletting behind the adding adjuvant, making every, to contain chemical compound (sweet) bisabolangelone (Bisabolangelone) be 0-30mg, (0-3mg of β-phellandrene) obtains tablet medicine to β-phellandrene.
4, the process for preparing medicine that contains extractive of Zi-Jin-Sha according to claim 3 is characterized in that: used adjuvant can be one or more in tablet medicines such as microcrystalline Cellulose, modified starch, magnesium stearate, ethyl cellulose, methylcellulose, hydroxyethyl-cellulose, hydroxypropyl emthylcellulose, the sodium carboxymethyl cellulose adjuvant commonly used.
5, the process for preparing medicine that contains extractive of Zi-Jin-Sha according to claim 1 and 2 is characterized in that:
Get 0～10 part of Radix Pternopetali vulgaris water extract, 0～10 part of Radix Pternopetali vulgaris volatile oil; Or get 0～10 part of Radix Pternopetali vulgaris ethanol extract, and be equivalent to crude drug in whole 10g for a, mix, get mixture 1000-10000 part, add adjuvant, granulate, granulate, encapsulated, make that to contain chemical compound (sweet) bisabolangelone in every capsules be 0-30mg, β-phellandrene 0-3mg.
6, the process for preparing medicine that contains extractive of Zi-Jin-Sha according to claim 5 is characterized in that: used adjuvant can be one or more in capsules such as microcrystalline Cellulose, modified starch, magnesium stearate, ethyl cellulose, methylcellulose, hydroxyethyl-cellulose, hydroxypropyl emthylcellulose, the sodium carboxymethyl cellulose adjuvant commonly used.
7, the application of extractive of Zi-Jin-Sha in analgesic.
8, the application of extractive of Zi-Jin-Sha in anti-ulcer medicament.
9, the application of extractive of Zi-Jin-Sha in the spasmolytic medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710051331 CN101002809A (en) | 2007-01-19 | 2007-01-19 | Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710051331 CN101002809A (en) | 2007-01-19 | 2007-01-19 | Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101002809A true CN101002809A (en) | 2007-07-25 |
Family
ID=38702335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710051331 Pending CN101002809A (en) | 2007-01-19 | 2007-01-19 | Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101002809A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104740472A (en) * | 2014-12-19 | 2015-07-01 | 徐菲 | Traditional Chinese medicine external application agent for treating postoperative cystospasm and nursing method |
| CN105380937A (en) * | 2015-10-26 | 2016-03-09 | 三峡大学 | Sesquiterpenoid anti-ulcer drug, preparation method and application |
| CN105412186A (en) * | 2014-09-03 | 2016-03-23 | 天津药物研究院 | Extracting preparation method for traditional Chinese medicinal material containing volatile oil and composition of traditional Chinese medicinal material |
-
2007
- 2007-01-19 CN CN 200710051331 patent/CN101002809A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105412186A (en) * | 2014-09-03 | 2016-03-23 | 天津药物研究院 | Extracting preparation method for traditional Chinese medicinal material containing volatile oil and composition of traditional Chinese medicinal material |
| CN104740472A (en) * | 2014-12-19 | 2015-07-01 | 徐菲 | Traditional Chinese medicine external application agent for treating postoperative cystospasm and nursing method |
| CN105380937A (en) * | 2015-10-26 | 2016-03-09 | 三峡大学 | Sesquiterpenoid anti-ulcer drug, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ganesh et al. | Phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS | |
| CN1307131C (en) | Antineoplastic extract of Chinese fan palm and its preparation method and uses | |
| CN103610722A (en) | Asarum heterotropoide extract, preparation method and application of extract | |
| Dhingra et al. | Evaluation of antidepressant-like activity of aqueous and ethanolic extracts of Terminalia bellirica Roxb. fruits in mice | |
| CN100435815C (en) | Medicine composition for treating pain and preparing method therefor | |
| CN101590065A (en) | Siberia Radix Polygalae sugar A1, Siberia Radix Polygalae sugar A5 and the tenuifoliside A application in preparation treatment depression product | |
| CN101002809A (en) | Medicine preparation containing extractive of Zi-Jin-Sha, preparing method and use thereof | |
| CN100478000C (en) | Application of valeriana wallichii and its extract in preparation of medicine for treating anxiety neurosis | |
| CN108434399A (en) | A kind of Chinese medicine composition and preparation method of anti-curing oncoma | |
| CN102861255A (en) | Preparation method and quality control method for medicine and preparation thereof for treating influenza | |
| CN1327859C (en) | Schisandra fruit extractive, its preparation process and purposes | |
| CN107837301A (en) | A kind of great Ye Betel extracts and preparation method and application | |
| CN102908519A (en) | Medicine for treating influenza as well as preparation method and quality detection method of preparation of medicine | |
| CN113754620B (en) | Lignan amide compound in fructus cannabis, and preparation method and application thereof | |
| CN107753531B (en) | Wenwang-herba violae extract and application thereof in preparing anti-cancer drugs | |
| CN103123343B (en) | Quality control method for Songling Xuemaikang capsules | |
| CN102078374A (en) | Tibetan medical dracocephalum heterophyllum benth extract and application thereof | |
| CN101375983B (en) | Chinese medicinal composition for treating dysmenorrhea and preparation method thereof | |
| CN104673493A (en) | Method for separating medicine pair volatile oil extracted by steam distillation method | |
| Ravi et al. | In vitro glucose uptake by isolated rat hemidiaphragm study of Aegle marmelos Correa root | |
| CN114601875A (en) | A radix Saxifragae extract, its preparation method and application in preparing medicine with effects of relaxing muscles and tendons and relieving pain | |
| CN104402897B (en) | Method for extracting high purity asarinin by supercritical carbon dioxide extraction method and application thereof | |
| Abu-Shandi et al. | A Quick GC/MS Method correlated with LC/MS/MS for the Identification of Medicinal Natural Products in Convolvulus arvensis: An Injury Healing Plant. | |
| CN109206392B (en) | Coumarin compound and preparation method and application thereof | |
| Lazzara et al. | Hypericum spp.: a resource from wild Mediterranean flora for the treatment of mild depression |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20070725 |