CN102266369B - Application of calliopsis extract in liver protection and antioxidation - Google Patents
Application of calliopsis extract in liver protection and antioxidation Download PDFInfo
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- CN102266369B CN102266369B CN2011101396178A CN201110139617A CN102266369B CN 102266369 B CN102266369 B CN 102266369B CN 2011101396178 A CN2011101396178 A CN 2011101396178A CN 201110139617 A CN201110139617 A CN 201110139617A CN 102266369 B CN102266369 B CN 102266369B
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Abstract
The invention relates to the technical fields of medicines and health-care food, and relates to new application of calliopsis extract for preparing liver protective and antioxidant medicaments and health-care foods. A preparation method for the calliopsis extract comprises the following steps of: crushing calliopsis capitulum, sieving with a 50-mesh sieve, refluxing and extracting by using 95 volume percent of ethanol twice and refluxing and extracting by using 50 volume percent of ethanol once, mixing extracting solution, and evaporating to dryness to obtain calliopsis total extract; and performing gradient elution on D-101 macroporous adsorbent resin, eluting by using 30 to 95 percent ethanol, collecting eluent, concentrating by a rotary evaporator, and drying by a drying box at constant temperature to obtain brown powder, wherein the yield is between 0.07 and 1.3 percent. Animal experiments discover that 30 to 95 percent ethanol eluate has the effects of liver protection and antioxidation and can be used for preparing the liver protective and antioxidant medicaments and the health-care foods.
Description
Technical field
The present invention relates to coreopsis extract protects the liver and the medicine of antioxidation and the application in the health food in preparation.
Technical background
Coreopsis basalis (Coreopsis tinctoria Nutt.) has another name called the snow chrysanthemum, is the dry capitulum of Compositae (Compositae) golden pheasant Chrysanthemum (Coreopsis), single-lobe, polyphyll or half polyphyll, and ligulate flower is yellow or golden yellow, florescence 6～JIUYUE.The Flos Caryophylli Coreopsis basalis belongs to the north temperate zone plant, originate in the North America, Florida state and Mexico state, China introduced a fine variety son as ornamental plant in 1936 and cultivates to Mount Lushan, the Southern Mountain and other places, various places are except confession is viewed and admired at present, also use all herbal medicine, and the food coloring of extraction from spend, the effect of heat-clearing and toxic substances removing and blood pressure lowering had.Comparatively extensive in the regional plantation of hotan at present, its dimension language name is called just complies with (Gulqai), trade name " snow chrysanthemum " in the Gu.Local resident's multiselect is quoted when tea with the snow chrysanthemum, and extraordinary use among the people basis is arranged.
Summary of the invention
The new purposes that the purpose of this invention is to provide coreopsis extract namely protects the liver in preparation, the medicine of antioxidation and the application in the health food.
Coreopsis basalis head titbit 500g, pulverized 50 mesh sieves, be 95% alcohol reflux 2 times and with 50% alcohol reflux 1 time with volume fraction respectively, solid-liquid ratio 1: 10, each 2h merges 3 times extracting solution, evaporate to dryness, get the Coreopsis basalis total extract, upper D-101 macroporous adsorbent resin carries out gradient elution, respectively water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, 95% ethanol elution, collect each eluent, concentrated through Rotary Evaporators, thermostatic drying chamber is dry, obtains brown ceramic powder, its yield is respectively: total extract, 16.8%, the water elution thing, 3.57%, 10% pure eluate, 0.95%, 30% pure eluate, 1.30%, 50% pure eluate, 0.09%, 70% pure eluate, 0.06%, 95% pure eluate, 0.07%.
Coreopsis basalis head titbit 500g pulverized 50 mesh sieves, was 95% alcohol reflux 2 times and with 50% alcohol reflux 1 time with volume fraction respectively, solid-liquid ratio 1: 10, each 2h merges 3 times extracting solution, evaporate to dryness gets the Coreopsis basalis total extract, and the D-101 macroporous adsorbent resin carries out gradient elution on the Coreopsis basalis total extract, use the 25%-75% ethanol elution, collect each eluent, concentrated through Rotary Evaporators, thermostatic drying chamber is dry, obtain brown ceramic powder, namely get coreopsis extract.
Coreopsis extract can be used for preparing protect the liver, medicine and the health food of antioxidation, aspect dosage form, can adopt various dosage forms, such as dosage forms such as tablet, capsule, granule, powder, slow releasing agent or pills.
Coreopsis extract can be used for preparing protect the liver, medicine and the health food of antioxidation, it is shaped as generic shape or special shape, also can be the dosage forms such as tablet, capsule or pill.
The coreopsis extract experimentation:
1, coreopsis extract Experimental Study on Antioxidation
1.2 coreopsis extract is to hydroxy radical (scavenging action test method OH)
Adopt Fenton reaction principle method, get 1mL 0.5mmol/L orthophenanthroline solution in test tube, the phosphate buffer (pH=7.4) that adds 2mL 0.2mol/L, abundant mixing, the 0.5mmol/L copperas solution that adds again the new preparation of 1mL adds new 0.1% hydrogen peroxide of preparing of 1mL, behind 37 ℃ of water-bath 60min behind the mixing, survey absorbance at the 510nm place, institute's light absorption value of surveying is the light absorption value that damages pipe; Do not damage pipe and replace hydrogen peroxide in the damage pipe with the 1mL distilled water, operational approach is with the damage pipe; Sample cell is with the distilled water in the 1mL sample replacement damage pipe, and operational approach is with the damage pipe.
Clearance rate=[(A
2-A
1)/(A
0-A
1)] * 100%
In the formula: A
0For not damaging the light absorption value of pipe; A
1Light absorption value for the damage pipe; A
2Light absorption value for sample cell.
1.3 coreopsis extract is to ultra-oxygen anion free radical (O
- 2) the scavenging action test method
Adopt the pyrogallol Autoxidation Method, get 50mmol/L Tris-HCl buffer solution (pH=8.2) 4.5mL, place 25 ℃ of water-bath preheating 20min, add respectively 1mL sample and 0.4mL 25mmol/L pyrogallol solution, in 25 ℃ of water-baths, behind the reaction 5min, add 8mol/LHCl 0.8mL cessation reaction behind the mixing.The autoxidation pipe replaces sample in the sample cell with the 1mL distilled water, the same sample cell of operational approach, at 325nm place mensuration light absorption value, with the Tris-HCl buffer of equal-volume pH value 8.2 as blank.
The clearance rate of superoxide anion=(A
0-A
1)/A
0* 100%
In the formula, A
0Light absorption value for the autoxidation pipe; A
1Light absorption value for sample cell.
1.4 coreopsis extract is to the scavenging action test method of DPPH free radical
With each extract of Coreopsis basalis and VC be made into 0.2,0.4,0.6,0.8, the solution for standby of 1.0g/L concentration.Get respectively the sample 1mL of each concentration in test tube, after the numbering, every test tube adds 6.5 * 10
-5Mol/LDPPH ethanol solution 4.0ml evenly mixes, and at room temperature lucifuge leaves standstill 30min.With the dehydrated alcohol zeroing, measure its absorbance A in the 517nm place.The blank group adopts dehydrated alcohol to substitute DPPH solution, and other reagent are identical with sample sets.Each experimental group all do 5 times parallel, get average.The DPPH free radical scavenging activity is calculated as follows.Clearance rate (%)=[1-(Ai-Aj)/A0] * 100%
A0 4.0mL6.5 * 10-5mol/LDPPH ethanol solution+1mL sample solvent
Ai 4.0mL6.5 * 10
-5Mol/LDPPH ethanol solution+1mL sample
Aj 4.0mL dehydrated alcohol+1mL sample
2, experimental result
2.1 the scavenging action of the p-OH free radical of coreopsis extract
Hydroxy radical is the most active free radical, and its reaction rate is exceedingly fast, and it is the maximum free radical of body harm.Experiment utilizes Fenton reaction principle method, and the Fenton reaction is the chemical reaction of modal generation hydroxy radical, H
2O
2Amount and the amount of the OH that produces of Fenton reaction be directly proportional, after giving electron acceptor, use the gress reagent colour development, the formation red material, the amount of its colour generation and OH is proportional.The p-OH free radical of coreopsis extract has obvious scavenging action as seen from Figure 1, and along with the increase of extract concentrations, the removing ability strengthens, and shows that the removing ability of the p-OH free radical of coreopsis extract and extract concentrations have obvious dose-effect relationship.30% ethanol extraction (clearance rate is 91.19%), 50% ethanol extraction (clearance rate is 86.19%) is removed ability and the V of free radical when concentration is 1.0g/L
C(clearance rate is 96.73%) is suitable.
2.2 coreopsis extract is to the scavenging action of O-2 free radical
Make reference with the blank group, survey the light absorption value of each concentration sample, draw coreopsis extract to the removing effect (Fig. 2) of ultra-oxygen anion free radical.As shown in Figure 2, when coreopsis extract concentration is 0.2～1.0g/L, also increase with the increase of the solution concentration clearance rate to ultra-oxygen anion free radical thereupon; When concentration reaches 1.0g/L, wherein 30% ethanol extraction and 50% ethanol extraction are respectively 55.65% and 97.90% to the clearance rate of ultra-oxygen anion free radical, 50% ethanol extraction is particularly remarkable to the clearance rate of ultra-oxygen anion free radical, obviously is better than positive control drug V
C(when concentration 1.0g/L, clearance rate is 68.89%).Illustrating that coreopsis extract has ultra-oxygen anion free radical under certain concentration conditions removes effect preferably.
2.3 coreopsis extract is to the scavenging action of DPPH free radical
DPPH is a kind of very stable free radical centered by N, at the 517nm place strong absworption peak is arranged, and DPPH is usually used to detect the antioxidant activity of antioxidant.When having free radical scavenger to exist, DPPH can and the proton that provides of scavenger in conjunction with formation DPPH-H, thereby make solution colour become yellow by purple.As can be seen from Figure 3,30% ethanol extraction, 50% ethanol extraction and 70% ethanol extraction are when concentration is 1.0g/L, to the clearance rate of free radical all surpassed 70% this show that coreopsis extract has the ability of very strong removing DPPH-and obvious dose dependent is arranged, experimental result explanation coreopsis extract is good proton donor, has significant antioxidation.
Experimental result shows, coreopsis extract has the effect of removing hydroxy radical, and along with the increase of extract concentrations, and the scavenging action of hydroxy radical is strengthened, and is obvious dose-effect relationship; Show that by pyrogallol autoxidation system the oxygen-derived free radicals in the obviously removing system of coreopsis extract shows extremely strong antioxidant activity.In addition, coreopsis extract also shows stronger antioxidation to the DPPH free radical.Confirm that by the scavenging action to free radical coreopsis extract is a kind of effective free radical scavenger, in the test mass concentration range, wherein 30% ethanol extraction and 50% ethanol extraction are to clearance rate and the V of DPPH and OH free radical
CQuite, 50% ethanol extraction is to O
- 2The clearance rate of free radical obviously is better than V
CShow that coreopsis extract is effective exogenous natural organic antioxidant.
3, coreopsis extract on carbon tetrachloride hepatic injury impact
Blank group and model group respectively gavage give the distilled water of 0.2ml/10g; The positive controls gavage gives the 400mg/kg bifendate; The administration group, every day, gavage gave coreopsis extract, continuous 5 days.After the last administration 1 hour, all the other respectively organize CCl4 (vegetable oil preparation) 0.2ml/10g of lumbar injection 0.1% except the blank group.Water is can't help in fasting, plucks eyeball after 24 hours and gets blood, measures ALT and AST activity with full automatic biochemical apparatus.
Annotate: # and blank group be p＜0.01 relatively, and * and model group are than p＜0.05
Model group serum alt, the active obviously rising (p＜0.01) of AST illustrate the modeling success.Administration group serum alt, AST level reduce (p＜0.05), show that coreopsis extract can suppress because of CCl
4Transaminase activity raises in the Mice Body that causes.
Description of drawings
Fig. 1 is the scavenging action figure of the p-OH free radical of coreopsis extract;
Fig. 2 is that coreopsis extract is to O
- 2The scavenging action figure of free radical;
Fig. 3 is that coreopsis extract is to the scavenging action figure of DPPH free radical.
Claims (3)
1. coreopsis extract, it is characterized in that Coreopsis basalis head titbit 500g, pulverized 50 mesh sieves, be 95% alcohol reflux 2 times and with 50% alcohol reflux 1 time with volume fraction respectively, solid-liquid ratio 1: 10, each 2h merges 3 times extracting solution, evaporate to dryness, get the Coreopsis basalis total extract, the D-101 macroporous adsorbent resin carries out gradient elution on the Coreopsis basalis total extract, uses the 25%-75% ethanol elution, collects each eluent, concentrated through Rotary Evaporators, thermostatic drying chamber is dry, obtains brown ceramic powder, and get final product.
2. the application of the medicine that protects the liver in preparation of coreopsis extract claimed in claim 1 and the health food that protects the liver is characterised in that coreopsis extract mixes with suitable carrier pharmaceutically, makes tablet, capsule, granule, powder, pill or slow releasing agent.
3. coreopsis extract claimed in claim 1 is in the application of preparation in the antioxidant functional food, is characterised in that coreopsis extract mixes with suitable carrier pharmaceutically, makes tablet, capsule, granule, powder, pill or slow releasing agent.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102696834A (en) * | 2012-06-21 | 2012-10-03 | 唐军涛 | Instant senecio cineraria tea cream and preparing process thereof |
| CN102764285B (en) * | 2012-08-01 | 2014-09-24 | 乌鲁木齐三高和药业有限公司 | Coreopsis tinctoria extract |
| CN102775811A (en) * | 2012-08-01 | 2012-11-14 | 乌鲁木齐三高和药业有限公司 | Process of extracting flavonoids from Coreopsis bicolor |
| CN102942466A (en) * | 2012-10-27 | 2013-02-27 | 青岛农业大学 | Chalcone compound as well as preparation method and application of chalcone compound |
| CN103830301A (en) * | 2012-11-26 | 2014-06-04 | 北京健旭康技术有限公司 | Flos chrysanthemi with liver tonifying function and application thereof |
| CN104523796B (en) * | 2014-12-22 | 2018-05-08 | 新疆大学 | Dichromatism coreopsis extract is as the application for preparing treatment leucoderma medicament |
| CN106665891B (en) * | 2017-01-09 | 2020-09-11 | 中国农业大学 | Preparation and use method of coreopsis tinctoria polyphenol |
| CN115581757A (en) * | 2022-11-07 | 2023-01-10 | 新疆农业大学 | Medicine for treating alcoholic liver injury and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102048786A (en) * | 2010-12-09 | 2011-05-11 | 新疆维吾尔自治区药物研究所 | Preparation and application of coreopsis extract |
| CN102058649A (en) * | 2009-11-18 | 2011-05-18 | 新疆维吾尔自治区药物研究所 | Application of extract of Coreopsis tinctoria Nuff. to preparation of medicaments or health foods for treating hypertension and hyperlipidemia |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058649A (en) * | 2009-11-18 | 2011-05-18 | 新疆维吾尔自治区药物研究所 | Application of extract of Coreopsis tinctoria Nuff. to preparation of medicaments or health foods for treating hypertension and hyperlipidemia |
| CN102048786A (en) * | 2010-12-09 | 2011-05-11 | 新疆维吾尔自治区药物研究所 | Preparation and application of coreopsis extract |
Non-Patent Citations (2)
| Title |
|---|
| 朱笃等.大孔树脂对金鸡菊黄酮吸附分离特性研究.《食品科学》.2006,420-423. * |
| 朱笃等.金鸡菊总黄酮的提取及含量测定.《食品科学》.2005,314-316. * |
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