CN102266369A - Application of calliopsis extract to liver protection and antioxidation - Google Patents
Application of calliopsis extract to liver protection and antioxidation Download PDFInfo
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- CN102266369A CN102266369A CN2011101396178A CN201110139617A CN102266369A CN 102266369 A CN102266369 A CN 102266369A CN 2011101396178 A CN2011101396178 A CN 2011101396178A CN 201110139617 A CN201110139617 A CN 201110139617A CN 102266369 A CN102266369 A CN 102266369A
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- coreopsis basalis
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Abstract
The invention relates to the technical fields of medicines and health-care food, and relates to new application of calliopsis extract for preparing liver protective and antioxidant medicaments and health-care foods. A preparation method for the calliopsis extract comprises the following steps of: crushing calliopsis capitulum, sieving with a 50-mesh sieve, refluxing and extracting by using 95 volume percent of ethanol twice and refluxing and extracting by using 50 volume percent of ethanol once, mixing extracting solution, and evaporating to dryness to obtain calliopsis total extract; and performing gradient elution on D-101 macroporous adsorbent resin, eluting by using 30 to 95 percent ethanol, collecting eluent, concentrating by a rotary evaporator, and drying by a drying box at constant temperature to obtain brown powder, wherein the yield is between 0.07 and 1.3 percent. Animal experiments discover that 30 to 95 percent ethanol eluate has the effects of liver protection and antioxidation and can be used for preparing the liver protective and antioxidant medicaments and the health-care foods.
Description
Technical field
The present invention relates to the Coreopsis basalis extract protects the liver and the medicine of antioxidation and the application in the health food in preparation.
Technical background
Coreopsis basalis (Coreopsis tinctoria Nutt.) has another name called the snow chrysanthemum, is the dry capitulum of Compositae (Compositae) golden pheasant Chrysanthemum (Coreopsis), single-lobe, polyphyll or half polyphyll, and ligulate flower is yellow or golden yellow, florescence 6～JIUYUE.The Flos Caryophylli Coreopsis basalis belongs to the north temperate zone plant, originate in the North America, Florida state and Mexico state, China introduced a fine variety son as ornamental plant in 1936 and cultivates to Mount Lushan, the Southern Mountain and other places, various places are except that confession is viewed and admired at present, also use all herbal medicine, and the food coloring that from spend, extracts, the effect of heat-clearing and toxic substances removing and blood pressure lowering had.Comparatively extensive in the regional plantation of hotan at present, its dimension language name is called just complies with (Gulqai), trade name " snow chrysanthemum " in the Gu.Local resident's multiselect is quoted when tea with the snow chrysanthemum, and extraordinary use among the people basis is arranged.
Summary of the invention
The new purposes that the purpose of this invention is to provide the Coreopsis basalis extract promptly protects the liver in preparation, the medicine of antioxidation and the application in the health food.
Coreopsis basalis head titbit 500g, pulverized 50 mesh sieves, be 95% alcohol reflux 2 times and with 50% alcohol reflux 1 time with volume fraction respectively, solid-liquid ratio 1: 10, each 2h merges 3 times extracting solution, evaporate to dryness, get the Coreopsis basalis total extract, last D-101 macroporous adsorbent resin carries out gradient elution, respectively water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, 95% ethanol elution, collect each eluent, concentrate through Rotary Evaporators, the thermostatic drying chamber drying obtains brown ceramic powder, its yield is respectively: total extract, 16.8%, the water elution thing, 3.57%, 10% pure eluate, 0.95%, 30% pure eluate, 1.30%, 50% pure eluate, 0.09%, 70% pure eluate, 0.06%, 95% pure eluate, 0.07%.
The Coreopsis basalis extract can be used for preparing protect the liver, the medicine and the health food of antioxidation, aspect dosage form, can adopt various dosage forms, as dosage forms such as tablet, granule, powder or pills.
The Coreopsis basalis extract can be used for preparing protect the liver, the medicine and the health food of antioxidation, it is shaped as common shape or special shape, also can be dosage forms such as tablet, capsule or pill.
Coreopsis basalis extract experimentation:
1, Coreopsis basalis extract Experimental Study on Antioxidation
1.2 the Coreopsis basalis extract is to hydroxy radical (scavenging action test method OH)
Adopt Fenton reaction principle method, get 1mL 0.5mmol/L orthophenanthroline solution in test tube, the phosphate buffer (pH=7.4) that adds 2mL 0.2mol/L, abundant mixing, the 0.5mmol/L copperas solution that adds the new preparation of 1mL again adds new 0.1% hydrogen peroxide of preparing of 1mL, behind 37 ℃ of water-bath 60min behind the mixing, survey absorbance at the 510nm place, institute's light absorption value of surveying is the light absorption value that damages pipe; Do not damage pipe and replace hydrogen peroxide in the damage pipe with the 1mL distilled water, operational approach is with the damage pipe; Sample cell is with the distilled water in the 1mL sample replacement damage pipe, and operational approach is with the damage pipe.
Clearance rate=[(A
2-A
1)/(A
0-A
1)] * 100%
In the formula: A
0For not damaging the light absorption value of pipe; A
1Light absorption value for the damage pipe; A
2Light absorption value for sample cell.
1.3 the Coreopsis basalis extract is to ultra-oxygen anion free radical (O
- 2) the scavenging action test method
Adopt pyrogallol autoxidation method, get 50mmol/L Tris-HCl buffer solution (pH=8.2) 4.5mL, place 25 ℃ of water-bath preheating 20min, add 1mL sample and 0.4mL 25mmol/L pyrogallol solution respectively, in 25 ℃ of water-baths, behind the reaction 5min, add 8mol/LHCl 0.8mL cessation reaction behind the mixing.The autoxidation pipe replaces sample in the sample cell with the 1mL distilled water, the same sample cell of operational approach, at 325nm place mensuration light absorption value, with the Tris-HCl buffer of equal-volume pH value 8.2 as blank.
The clearance rate of superoxide anion=(A
0-A
1)/A
0* 100%
In the formula, A
0Light absorption value for the autoxidation pipe; A
1Light absorption value for sample cell.
1.4 the Coreopsis basalis extract is to DPPH measured by esr technique test method
With each extract of Coreopsis basalis and VC be made into 0.2,0.4,0.6,0.8, the solution for standby of 1.0g/L concentration.Get each concentrations samples 1mL respectively in test tube, after the numbering, every test tube adds 6.5 * 10
-5Mol/LDPPH ethanol solution 4.0ml uniform mixing, at room temperature lucifuge leaves standstill 30min.With the dehydrated alcohol zeroing, measure its absorbance A in the 517nm place.The blank group adopts dehydrated alcohol to substitute DPPH solution, and other reagent are identical with sample sets.Each experimental group all do 5 times parallel, get average.The DPPH free radical scavenging activity is calculated as follows.Clearance rate (%)=[1-(Ai-Aj)/A0] * 100%
A0 4.0mL6.5 * 10-5mol/LDPPH ethanol solution+1mL sample solvent
Ai 4.0mL6.5 * 10
-5Mol/LDPPH ethanol solution+1mL sample
Aj 4.0mL dehydrated alcohol+1mL sample
2, experimental result
2.1 the Coreopsis basalis extract is right-the OH measured by esr technique
Hydroxy radical is the most active free radical, and its reaction rate is exceedingly fast, and it is the maximum free radical of body harm.Experiment utilizes Fenton reaction principle method, and the Fenton reaction is the chemical reaction of modal generation hydroxy radical, H
2O
2Amount and the amount of the OH that produces of Fenton reaction be directly proportional, after giving electron acceptor, use the gress reagent colour development, the formation red material, the amount of its colour generation and OH is proportional.As seen from Figure 1 the Coreopsis basalis extract right-the OH free radical has tangible scavenging action, and along with the increase of extract concentrations, the removing ability strengthens, show the Coreopsis basalis extract right-the removing ability and the extract concentrations of OH free radical have tangible dose-effect relationship.30% ethanol extraction (clearance rate is 91.19%), 50% ethanol extraction (clearance rate is 86.19%) is removed the ability and the V of free radical during for 1.0g/L in concentration
C(clearance rate is 96.73%) is suitable.
2.2 the Coreopsis basalis extract is to O
- 2Measured by esr technique
Make reference with the blank group, survey the light absorption value of each concentration sample, draw the removing effect (Fig. 2) of Coreopsis basalis extract ultra-oxygen anion free radical.As shown in Figure 2, when the Coreopsis basalis extract concentrations is 0.2～1.0g/L, also increase with the increase of solution concentration clearance rate to ultra-oxygen anion free radical thereupon; When concentration reaches 1.0g/L, wherein 30% ethanol extraction and 50% ethanol extraction are respectively 55.65% and 97.90% to the clearance rate of ultra-oxygen anion free radical, 50% ethanol extraction is particularly remarkable to the clearance rate of ultra-oxygen anion free radical, obviously is better than positive control drug V
C(when concentration 1.0g/L, clearance rate is 68.89%).Illustrating that the Coreopsis basalis extract has ultra-oxygen anion free radical under certain concentration conditions removes effect preferably.
2.3 the Coreopsis basalis extract is to the DPPH measured by esr technique
DPPH be a kind of very stable be the free radical at center with N, at the 517nm place strong absworption peak is arranged, DPPH is used to detect the antioxidant activity of antioxidant usually.When free radical scavenger exists, DPPH can and the proton that provides of scavenger in conjunction with formation DPPH-H, thereby make solution colour become yellow by purple.As can be seen from Figure 3,30% ethanol extraction, 50% ethanol extraction and 70% ethanol extraction are when concentration is 1.0g/L, to the clearance rate of free radical all surpassed 70% this show that the Coreopsis basalis extract has the ability of very strong removing DPPH and tangible dose dependent is arranged, experimental result explanation Coreopsis basalis extract is good proton donor, has significant antioxidant role.
Experimental result shows, the Coreopsis basalis extract has the effect of removing hydroxy radical, and along with the increase of extract concentrations, and the scavenging action of hydroxy radical is strengthened, and is tangible dose-effect relationship; Show that by pyrogallol autoxidation system the oxygen-derived free radicals in the obviously removing system of Coreopsis basalis extract shows extremely strong antioxidant activity.In addition, the Coreopsis basalis extract also shows stronger antioxidation to the DPPH free radical.By measured by esr technique is confirmed that the Coreopsis basalis extract is a kind of effective free radical scavenger, in the test mass concentration range, wherein 30% ethanol extraction and 50% ethanol extraction are to the clearance rate and the V of DPPH and OH free radical
CQuite, 50% ethanol extraction is to O
- 2The clearance rate of free radical obviously is better than V
CShow that the Coreopsis basalis extract is effective exogenous natural organic antioxidant.
3, the Coreopsis basalis extract to carbon tetrachloride hepatic injury influence
Blank group and model group are irritated the distilled water that stomach gives 0.2ml/10g respectively; Positive controls is irritated stomach and is given 400mg/kg bifendate; The administration group is irritated stomach every day and is given the Coreopsis basalis extract, continuous 5 days.After the last administration 1 hour, all the other respectively organize CCl4 (vegetable oil preparation) 0.2ml/10g of lumbar injection 0.1% except that the blank group.Water is can't help in fasting, plucks eyeball after 24 hours and gets blood, measures ALT and AST activity with full automatic biochemical apparatus.
Annotate: # and blank group be p＜0.01 relatively, and * and model group are than p＜0.05
Model group serum alt, the active obviously rising (p＜0.01) of AST illustrate the modeling success.Administration group serum alt, AST level reduce (p＜0.05), show that the Coreopsis basalis extract can suppress because of CCl
4Transaminase activity raises in the mice body that causes.
Description of drawings
Fig. 1 be the Coreopsis basalis extract right-OH measured by esr technique figure;
Fig. 2 is that the Coreopsis basalis extract is to O
- 2Measured by esr technique figure;
Fig. 3 is that the Coreopsis basalis extract is to DPPH measured by esr technique figure.
Claims (2)
1. the described to specifications Coreopsis basalis extract that obtains, it is characterized in that the D-101 macroporous adsorbent resin carries out gradient elution on the Coreopsis basalis total extract, use the 25%-75% ethanol elution, collect each eluent, concentrate through Rotary Evaporators, the thermostatic drying chamber drying, obtain brown ceramic powder, this extract can be used for preparing medicine that protects the liver and the health food that protects the liver, be characterised in that the Coreopsis basalis extract can mix with suitable carrier pharmaceutically, makes tablet, capsule, granule, powder, pill or slow releasing agent etc.
2. the described to specifications Coreopsis basalis extract that obtains, it is characterized in that the D-101 macroporous adsorbent resin carries out gradient elution on the Coreopsis basalis total extract, use the 25%-75% ethanol elution, collect each eluent, concentrate the thermostatic drying chamber drying through Rotary Evaporators, obtain brown ceramic powder, this extract can be used for preparing anti-oxidation health food, is characterised in that the Coreopsis basalis extract can mix with suitable carrier pharmaceutically, makes tablet, capsule, granule, powder, pill or slow releasing agent etc.
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| CN2011101396178A CN102266369B (en) | 2011-05-27 | 2011-05-27 | Application of calliopsis extract in liver protection and antioxidation |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696834A (en) * | 2012-06-21 | 2012-10-03 | 唐军涛 | Instant senecio cineraria tea cream and preparing process thereof |
| CN102764285A (en) * | 2012-08-01 | 2012-11-07 | 乌鲁木齐三高和药业有限公司 | Coreopsis tinctoria extract |
| CN102775811A (en) * | 2012-08-01 | 2012-11-14 | 乌鲁木齐三高和药业有限公司 | Process of extracting flavonoids from Coreopsis bicolor |
| CN102942466A (en) * | 2012-10-27 | 2013-02-27 | 青岛农业大学 | Chalcone compound as well as preparation method and application of chalcone compound |
| CN103830301A (en) * | 2012-11-26 | 2014-06-04 | 北京健旭康技术有限公司 | Flos chrysanthemi with liver tonifying function and application thereof |
| CN104523796A (en) * | 2014-12-22 | 2015-04-22 | 新疆大学 | Application of coreopsis tinctoria extract in preparation of medicine for treating leucoderma |
| CN106665891A (en) * | 2017-01-09 | 2017-05-17 | 中国农业大学 | Preparation and use method of snow chrysanthemum polyphenol |
| CN115581757A (en) * | 2022-11-07 | 2023-01-10 | 新疆农业大学 | Medicine for treating alcoholic liver injury and preparation method thereof |
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| CN102048786A (en) * | 2010-12-09 | 2011-05-11 | 新疆维吾尔自治区药物研究所 | Preparation and application of coreopsis extract |
| CN102058649A (en) * | 2009-11-18 | 2011-05-18 | 新疆维吾尔自治区药物研究所 | Application of extract of Coreopsis tinctoria Nuff. to preparation of medicaments or health foods for treating hypertension and hyperlipidemia |
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2011
- 2011-05-27 CN CN2011101396178A patent/CN102266369B/en not_active Expired - Fee Related
Patent Citations (2)
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| CN102058649A (en) * | 2009-11-18 | 2011-05-18 | 新疆维吾尔自治区药物研究所 | Application of extract of Coreopsis tinctoria Nuff. to preparation of medicaments or health foods for treating hypertension and hyperlipidemia |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696834A (en) * | 2012-06-21 | 2012-10-03 | 唐军涛 | Instant senecio cineraria tea cream and preparing process thereof |
| CN102764285A (en) * | 2012-08-01 | 2012-11-07 | 乌鲁木齐三高和药业有限公司 | Coreopsis tinctoria extract |
| CN102775811A (en) * | 2012-08-01 | 2012-11-14 | 乌鲁木齐三高和药业有限公司 | Process of extracting flavonoids from Coreopsis bicolor |
| CN102764285B (en) * | 2012-08-01 | 2014-09-24 | 乌鲁木齐三高和药业有限公司 | Coreopsis tinctoria extract |
| CN102942466A (en) * | 2012-10-27 | 2013-02-27 | 青岛农业大学 | Chalcone compound as well as preparation method and application of chalcone compound |
| CN103830301A (en) * | 2012-11-26 | 2014-06-04 | 北京健旭康技术有限公司 | Flos chrysanthemi with liver tonifying function and application thereof |
| CN104523796A (en) * | 2014-12-22 | 2015-04-22 | 新疆大学 | Application of coreopsis tinctoria extract in preparation of medicine for treating leucoderma |
| CN104523796B (en) * | 2014-12-22 | 2018-05-08 | 新疆大学 | Dichromatism coreopsis extract is as the application for preparing treatment leucoderma medicament |
| CN106665891A (en) * | 2017-01-09 | 2017-05-17 | 中国农业大学 | Preparation and use method of snow chrysanthemum polyphenol |
| CN115581757A (en) * | 2022-11-07 | 2023-01-10 | 新疆农业大学 | Medicine for treating alcoholic liver injury and preparation method thereof |
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|---|---|
| CN102266369B (en) | 2013-03-20 |
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