CN107029150A - A kind of barley young leaf extract and its preparation method and application - Google Patents
A kind of barley young leaf extract and its preparation method and application Download PDFInfo
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- CN107029150A CN107029150A CN201710306155.1A CN201710306155A CN107029150A CN 107029150 A CN107029150 A CN 107029150A CN 201710306155 A CN201710306155 A CN 201710306155A CN 107029150 A CN107029150 A CN 107029150A
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- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 106
- 239000000284 extract Substances 0.000 title claims abstract description 79
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 240000005979 Hordeum vulgare Species 0.000 title 1
- 241000209219 Hordeum Species 0.000 claims abstract description 106
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 239000000843 powder Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 150000003254 radicals Chemical class 0.000 claims abstract description 19
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 238000002525 ultrasonication Methods 0.000 claims abstract description 8
- 238000000527 sonication Methods 0.000 claims abstract description 5
- 239000003480 eluent Substances 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims 1
- 238000002835 absorbance Methods 0.000 description 7
- 230000002000 scavenging effect Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000002292 Radical scavenging effect Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000005445 natural material Substances 0.000 description 5
- 238000000605 extraction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 231100000957 no side effect Toxicity 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000209510 Liliopsida Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of barley young leaf extract, comprise the following steps：Take barley young leaf dry powder, add distilled water, be well mixed, mixed liquid is placed in the 4min of ultrasonication 2 in ultrasonic cell disruptor, wherein, ultrasonic frequency is 25 40kHz, and the solid-liquid ratio of barley young leaf dry powder and distilled water is 1g:10‑20ml；The absolute ethyl alcohol of 23 times of volumes is added in liquid after sonication, microwave treatment is then carried out, microwave power is 500 700W, and the microwave time is 4 6min；Liquid after microwave treatment is placed into 2 3h, centrifuged, supernatant is obtained, the supernatant is dried in vacuo, barley young leaf extract is obtained.Invention additionally discloses the application of a kind of barley young leaf extract and extract.The barley young leaf extract of the present invention can be purged to free radical.
Description
Technical field
The present invention relates to biomedicine technical field, more particularly to a kind of barley young leaf extract and preparation method thereof and
Using.
Background technology
Barley is grass family Hordeum monocotyledon, is a kind of medicinal and edible plant.Contain what is enriched in barley wheat seeding
Nutriment, at present, the fashionable multiple countries of the food being made using wheat seeding as raw material.Barley young leaf is barley growth to 20-30
The seedling of centimetre, nutriment contained by the seedling leaves in this period enriches the most, and research shows, the material in barley young leaf has
Oxidation resistant effect, can remove free radical, protect human body from the damage of free radical.
Free radical is also known as free radical, is group or atom with uncoupled electron, free radical can by electro transfer plus
Into matter interactions such as polysaccharide, amino acid, protein, nucleic acid, the lipids in the various ways and organism such as, dehydrogenation, cause
These material oxidative damages, further make meronecrosis or mutation.
How a kind of pure natural substance for removing free radical is provided, be radicals scavenging field urgent problem to be solved it
One.
The content of the invention
, can the present invention solves the technical problem of a kind of barley young leaf extract and its preparation method and application is provided
Pure natural barley young leaf extract is prepared, the extract has radicals scavenging function.
In order to solve the above technical problems, the present invention provides a kind of preparation method of barley young leaf extract, including following step
Suddenly：Barley young leaf is dried, and is crushed to 60-80 mesh, barley young leaf dry powder is obtained；Barley young leaf dry powder is taken, adds and steams
Distilled water, is well mixed, mixed liquid is placed in into ultrasonication 2-4min in ultrasonic cell disruptor, wherein, ultrasound
Wave frequency rate is 25-40kHz, and the solid-liquid ratio of barley young leaf dry powder and distilled water is 1g:10-20ml；Liquid after sonication
The absolute ethyl alcohol of 2-3 times of volume is added in body, microwave treatment is then carried out, microwave power is 500-700W, and the microwave time is 4-
6min；Liquid after microwave treatment is placed into 2-3h, centrifuged, supernatant is obtained, the supernatant is dried in vacuo, obtained
Obtain barley young leaf extract.
Wherein, barley young leaf dry powder is 70 mesh.
Wherein, ultrasonic treatment time is that 3min, frequency are 35kHz.
Wherein, the solid-liquid ratio of barley young leaf dry powder and distilled water is 1g:15ml.
Wherein, after ultrasonication liquid and the volume ratio of absolute ethyl alcohol is 1:2.5.
Wherein, microwave treatment time is that 5min, microwave power are 600W.
Wherein, the barley young leaf extract of acquisition is dissolved using distilled water, then upper large pore resin absorption column, with
Distillation be washed to efflux it is colourless after, then with 75-85% ethanol elution, the elution amount of eluent is 3-4BV, collects eluent,
Eluent is dried, the barley young leaf extract of purifying is obtained.
In order to solve the above technical problems, the present invention provides the barley young leaf extraction that a kind of above-mentioned preparation method is prepared
Thing.
In order to solve the above technical problems, the present invention provides a kind of barley young leaf extract as removing free radical medicine or guarantor
The application of strong product.Specifically, barley young leaf extract is tablet, capsule as the formulation of removing free radical medicine or health products
Agent, granule or powder.
The beneficial effects of the invention are as follows：It is different from the situation of prior art, the preparation of barley young leaf extract of the invention
Method comprises the following steps：Barley young leaf is dried, and is crushed to 60-80 mesh, barley young leaf dry powder is obtained；If taking barley
Leaf dried powder, adds distilled water, is well mixed, mixed liquid is placed in into ultrasonication 2- in ultrasonic cell disruptor
4min, wherein, ultrasonic frequency is 25-40kHz, and the solid-liquid ratio of barley young leaf dry powder and distilled water is 1g:10-20ml；Super
The absolute ethyl alcohol of 2-3 times of volume is added in liquid after sonicated, microwave treatment is then carried out, microwave power is 500-
700W, the microwave time is 4-6min；Liquid after microwave treatment is placed into 2-3h, centrifuged, supernatant is obtained, to the supernatant
Liquid is dried in vacuo, and obtains barley young leaf extract.Barley young leaf extract prepared by the above method is pure natural substance, can
Free radical is purged.Above-mentioned preparation method has the characteristics that：Simply, it is easy to operate；Due to passing through to barley young leaf cell
Ultrasonic wave, microwave are fully crushed, and preferably the material in barley young leaf can be extracted, and can reduce extraction time, raising
Extraction efficiency；The barley young leaf extract prepared has higher recovery rate, and nontoxic, harmless；Utilize macroporous absorbent resin
Post can prepare purer barley young leaf extract.
Embodiment
Embodiment 1
The preparation method of barley young leaf extract comprises the following steps：Barley young leaf is dried, and is crushed to 70 mesh, is obtained
Barley young leaf dry powder；Barley young leaf dry powder is taken, distilled water is added, is well mixed, mixed liquid is placed in supersonic cell
Ultrasonication 3min in pulverizer, wherein, ultrasonic frequency is 35kHz, and the solid-liquid ratio of barley young leaf dry powder and distilled water is
1g:15ml；The absolute ethyl alcohol of 2.5 times of volumes is added in liquid after sonication, microwave treatment, microwave work(is then carried out
Rate is 600W, and the microwave time is 5min；Liquid after microwave treatment is placed into 2.5h, centrifuged, supernatant is obtained, on this
Clear liquid is dried in vacuo, and obtains barley young leaf extract.
Barley young leaf extract manufactured in the present embodiment can be purged to free radical, right because it is pure natural substance
Human body has no side effect.
Embodiment 2
The preparation method of barley young leaf extract comprises the following steps：Barley young leaf is dried, and is crushed to 70 mesh, is obtained
Barley young leaf dry powder；Barley young leaf dry powder is taken, distilled water is added, is well mixed, mixed liquid is placed in supersonic cell
Ultrasonication 2.5min in pulverizer, wherein, ultrasonic frequency is 30kHz, barley young leaf dry powder and distilled water solid-liquid ratio
For 1g:15ml；The absolute ethyl alcohol of 2 times of volumes is added in liquid after sonication, microwave treatment, microwave work(is then carried out
Rate is 550W, and the microwave time is 4.5min；Liquid after microwave treatment is placed into 2.5h, centrifuged, supernatant is obtained, to this
Supernatant is dried in vacuo, and obtains barley young leaf extract.
Barley young leaf extract manufactured in the present embodiment can be purged to free radical, right because it is pure natural substance
Human body has no side effect.
Embodiment 3
On the basis of embodiment 1, the barley young leaf extract of acquisition is dissolved using distilled water, then upper macroporous absorption
Resin column, with distill be washed to efflux it is colourless after, then with 80% ethanol elution, the elution amount of eluent is 3.5BV, is collected
Eluent, eluent is dried, and obtains the barley young leaf extract of purifying.
Embodiment 4
On the basis of embodiment 2, the barley young leaf extract of acquisition is dissolved using distilled water, then upper macroporous absorption
Resin column, with distill be washed to efflux it is colourless after, then with 80% ethanol elution, the elution amount of eluent is 3.5BV, is collected
Eluent, eluent is dried, and obtains the barley young leaf extract of purifying.
Embodiment 5
The preparation of barley young leaf extract tablet：Barley young leaf extract one-shot tabletting prepared by embodiment 1 or embodiment 2
Machine breaks into a diameter of 7mm, and weight is 350mg piece, produces barley young leaf extract tablet.
Embodiment 6
The preparation of barley young leaf extract capsule agent：Barley young leaf extract prepared by embodiment 1 or embodiment 2 is with 350mg
No. 1 capsulae enterosolubilis is packed into, barley young leaf extract capsule agent is produced.
Embodiment 7
The preparation of barley young leaf extract particles agent：Barley young leaf extract prepared by embodiment 1 or embodiment 2, which adds water, to be made
Softwood, crosses 15 mesh sieves and is granulated, barley young leaf extract particles agent is produced after drying.
Embodiment 8
The preparation of barley young leaf extract powder：Barley young leaf extract prepared by embodiment 1 or embodiment 2 crosses 250 mesh sieves,
Produce barley young leaf extract powder.
Below by the radicals scavenging effect of description of test barley young leaf extract of the present invention.
Weigh DPPH（1,1- diphenyl -2- trinitrophenyl-hydrazines）, dissolved using ethanol, it is 0.1mmol/ to make its concentration
L；Barley young leaf extract, the barley young leaf extract of the preparation of embodiment 2 of the preparation of embodiment 1 are weighed, is carried out using distilled water
Dissolving, is respectively prepared the solution of 10 concentration gradients, concentration gradient is specially：0.2mg/ml、0.4mg/ml、0.6mg/ml、
0.8mg/ml、1.0mg/ml、1.2mg/ml、1.4mg/ml、1.6mg/ml、1.8mg/ml、2.0mg/ml；It is V to take volume1's
DPPH solution, adds isometric absolute ethyl alcohol, mixes, room temperature, avoid light place 20min, and absorbance A is surveyed at 517nm0。
Volume is separately added into 10 solution that barley young leaf extract prepared by embodiment 1 is made for V1DPPH it is molten
Liquid and absolute ethyl alcohol, according to the method described above, survey absorbance A1, wherein, the absolute ethyl alcohol of addition and the volume of extract solution it
With for V1；In each extract solution, absolute ethyl alcohol is added, wherein, the absolute ethyl alcohol of addition and the volume of extract solution are equal
For V1, according to the method described above, survey absorbance A2；Clearance rate is calculated according to absorbance：Clearance rate=[A0-（A1-A2）]/A0×100%。
Volume is separately added into 10 solution that barley young leaf extract prepared by embodiment 2 is made for V1DPPH it is molten
Liquid and absolute ethyl alcohol, according to the method described above, survey absorbance A1, wherein, the absolute ethyl alcohol of addition and the volume of extract solution it
With for V1；In each extract solution, absolute ethyl alcohol is added, wherein, the absolute ethyl alcohol of addition and the volume of extract solution are equal
For V1, according to the method described above, survey absorbance A2；Clearance rate is calculated according to absorbance：Clearance rate=[A0-（A1-A2）]/A0×100%。
By abscissa of barley young leaf extract concentrations, clearance rate be that ordinate draws curve, linear according to curve returns
Return equation, calculate the 503nhibiting concentration for removing free radical（IC50）.
Experimental result：The IC of barley young leaf extract prepared by embodiment 1 to the Scavenging activity of free radical50For 1.50mg/
Ml, the IC of barley young leaf extract prepared by embodiment 2 to the Scavenging activity of free radical50For 1.52mg/ml.According to experimental result
As can be seen that barley young leaf extract prepared by embodiment 1 and embodiment 2 shows strong radical scavenging activity, with good
Good antioxidation activity.
IC of the barley young leaf general flavone to radical scavenging activity is calculated using above-mentioned experimental method50, by calculating, IC50
It is worth for 3.16mg/ml.According to result of calculation, it can be seen that barley young leaf extract prepared by embodiment 1 or embodiment 2 is compared with barley
If leaf flavonoids have more preferable radical scavenging activity.
The barley young leaf extract prepared using above-mentioned experimental method calculating embodiment 3, embodiment 4 is to radical scavenging activity
The IC of power50, the IC of barley young leaf extract prepared by embodiment 3 to the Scavenging activity of free radical50For 1.47mg/ml, embodiment
IC of the 4 barley young leaf extracts prepared to the Scavenging activity of free radical50For 1.48mg/ml.According to experimental result as can be seen that
Barley young leaf extract prepared by embodiment 3, embodiment 4 has compared with barley young leaf extract prepared by embodiment 1, embodiment 2
More preferable radical scavenging activity.
In summary, barley young leaf extract of the invention can be purged to free radical, and it is pure natural substance, right
Human body has no side effect.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent transformation that bright description is made, or other related technical fields are directly or indirectly used in, similarly it is included in
In the scope of patent protection of the present invention.
Claims (10)
1. a kind of preparation method of barley young leaf extract, it is characterised in that comprise the following steps：
Barley young leaf is dried, and is crushed to 60-80 mesh, barley young leaf dry powder is obtained；
The barley young leaf dry powder is taken, distilled water is added, is well mixed, mixed liquid is placed in ultrasonic cell disruptor
Middle ultrasonication 2-4min, wherein, ultrasonic frequency is 25-40kHz, the solid-liquid ratio of the barley young leaf dry powder and distilled water
For 1g:10-20ml；
The absolute ethyl alcohol of 2-3 times of volume is added in liquid after sonication, microwave treatment is then carried out, microwave power is
500-700W, the microwave time is 4-6min；
Liquid after microwave treatment is placed into 2-3h, centrifuged, supernatant is obtained, the supernatant is dried in vacuo, obtained
Obtain barley young leaf extract.
2. the preparation method of barley young leaf extract according to claim 1, it is characterised in that the barley young leaf dry powder
For 70 mesh.
3. the preparation method of barley young leaf extract according to claim 1, it is characterised in that ultrasonic treatment time is
3min, frequency are 35kHz.
4. the preparation method of barley young leaf extract according to claim 1, it is characterised in that the barley young leaf dry powder
Solid-liquid ratio with distilled water is 1g:15ml.
5. the preparation method of barley young leaf extract according to claim 1, it is characterised in that the liquid after ultrasonication
The volume ratio of body and absolute ethyl alcohol is 1:2.5.
6. the preparation method of barley young leaf extract according to claim 1, it is characterised in that microwave treatment time is
5min, microwave power are 600W.
7. the preparation method of barley young leaf extract according to claim 1, it is characterised in that using distilled water to described
Barley young leaf extract is dissolved, then upper large pore resin absorption column, with distill be washed to efflux it is colourless after, then with 75-
85% ethanol elution, the elution amount of eluent is 3-4BV, collects eluent, eluent is dried, obtain the big of purifying
If wheat leaf extract.
8. the barley young leaf extract that the preparation method described in claim 1-7 any one is prepared.
9. the barley young leaf extract described in claim 8 is used as the application for removing free radical medicine or health products.
10. application according to claim 9, it is characterised in that the barley young leaf extract is used as removing free radical medicine
The formulation of thing or health products is tablet, capsule, granule or powder.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109170643A (en) * | 2018-08-28 | 2019-01-11 | 北海市海庆兄弟水产有限公司 | One seed shrimp cunning and preparation method thereof |
| CN109221983A (en) * | 2018-09-29 | 2019-01-18 | 北海市海庆兄弟水产有限公司 | Shrimp Rong and preparation method thereof |
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| CN102060825A (en) * | 2009-11-13 | 2011-05-18 | 天津市食品研究所有限公司 | Method for extracting flavone from young barley grass |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060825A (en) * | 2009-11-13 | 2011-05-18 | 天津市食品研究所有限公司 | Method for extracting flavone from young barley grass |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170643A (en) * | 2018-08-28 | 2019-01-11 | 北海市海庆兄弟水产有限公司 | One seed shrimp cunning and preparation method thereof |
| CN109221983A (en) * | 2018-09-29 | 2019-01-18 | 北海市海庆兄弟水产有限公司 | Shrimp Rong and preparation method thereof |
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Application publication date: 20170811 |