CN102872195B - A kind of preparation method and application thereof of Chinese cassia tree total polyphenols extract - Google Patents
A kind of preparation method and application thereof of Chinese cassia tree total polyphenols extract Download PDFInfo
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- CN102872195B CN102872195B CN201210434838.2A CN201210434838A CN102872195B CN 102872195 B CN102872195 B CN 102872195B CN 201210434838 A CN201210434838 A CN 201210434838A CN 102872195 B CN102872195 B CN 102872195B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 32
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Chinese cassia tree Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, and Determination of Cinnamaldehyde is lower than 5%. This Chinese cassia tree total polyphenols extract is to extract from Chinese cassia tree, makes through macroporous resin column purifying, and preparation method is simple, and extraction efficiency is high, and production cost is low, safe, is suitable for suitability for industrialized production. This Chinese cassia tree total polyphenols extract can be prepared into and be used for the treatment of, prevents the pharmaceutical preparations such as the oral tablet, oral disnitegration tablet, lozenge, dispersing tablet, hard capsule, soft capsule, sustained release preparation, granule, pill, dripping pill, oral liquid, dry suspensoid agent of diabetes and complication thereof.
Description
Technical field
The invention belongs to pharmaceutical preparations technology field, relate to a kind of active component extracting from Chinese traditional medicine cinnamon, the invention still further relates toThe preparation method of this active component and medical usage thereof. Relate to specifically a kind of Chinese cassia tree total polyphenols extract, this extract adoptsThe method of alcohol extract, purification with macroreticular resin makes, and can be prepared into and be used for the treatment of, prevents diabetes and complication thereofPharmaceutical preparation.
Background technology
Diabetes are one diseases widely, and the incidence of disease is all rising every year, and the whole world is the patient of diabetes of approximately 1.25 hundred million left and right at presentPerson, has become one of three large difficult disease in the present age. China is existing diabetic approximately 4,000 ten thousand people at present, while diabetic populationDevelopment is just being tending towards becoming younger, and diabetes itself are also with a lot of complication in addition, and hypertension, coronary heart disease etc., have a strong impact on patientQuality of life. In the prevention and treatment field of diabetes, in the urgent need to develop a kind of good effect, side effect low, haveThe natural drug that multiple effect and cost are low.
Chinese cassia tree is the dry bark of canella Chinese cassia tree CinnamomumcassiaPresl, the Shennong's Herbal that begins to be loaded in,China has the medicinal history of 2000 at least, and its acrid-sweet flavor, hot greatly has the effects such as the fire of benefit is supporing yang, let the fire back to its origin, eliminating cold to stop pain,Fecund is in provinces such as Yunnan Province of China, Guangxi, Guangdong, Fujian. Chinese cassia tree is the vegetable material of the dietotherapeutic of the Ministry of Public Health of China announcement,Both be widely used as flavorant among the people, on tcm clinical practice, used as medicine again. The contained chemical composition of Chinese cassia tree has Chinese cassia treeOil, polyphenol, organic acid, tannin, polysaccharide, cinnamoside, cassia bark glycosides, steroidal, lignin etc.
More external scientists find that through a large amount of experiments and research the effect of Chinese cassia tree Polyphenols extract for treating diabetic is remarkable,All obtained success with it mouse and human body, and the external multiplex ground cinnamon of patient or Chinese cassia tree Polyphenols extract are as auxiliaryHypoglycemic medicine is taken, and obtains good effect. Research is found, is contained a kind of material that is similar to insulin, Yi Zhongduo in Chinese cassia treeAldehydes matter, can improve insulin to the stabilization of blood sugar level and reduce insulin resistance. The mode that realizes this type of function is:1. activation energy stimulates the enzyme of insulin receptor. 2. be that insulin signaling pathway in skeletal muscle tissue is more unimpeded.
In the disclosed patent application about Chinese cassia tree of Chinese patent communique, there are following several parts to relate to cinnamomum cassia extract and at sugarUrinate the application of disease aspect. Application number is 200610117512.1, name be called " preparation method of cinnamomum cassia extract, cinnamomum cassia extract,Its composition and purposes " patent application relate to the preparation method of cinnamomum cassia extract, comprise the following steps: a) that Chinese cassia tree is formerMaterial mixes with aqueous solvent; B) extracting is by a) mixture of gained of step; C) separate residue, obtain cinnamomum cassia extract. GainedIn cinnamomum cassia extract, contain cinnamyl alcohol, cinnamic acid, cinnamic acid, eugenol etc., outward appearance is yellow clear liquid, and pH is 4.6 left sidesRight. This invention also provides the composition that contains this extract. The cinnamomum cassia extract of this invention can be used for preparation treatment and has the blood of fallingSugar and the double effects of reducing blood lipid and there are medicine or the health products of the function of protecting the liver. The related cinnamomum cassia extract of this invention is mainlyCortex Cinnamomi volatile oil.
Application number is 200610201217.4, and name is called " Chinese cassia tree is purposes and product and its preparation method in diabetes in treatment "Patent application relates to medicine, health products or the food for the preparation of prevention, treatment diabetes by Chinese cassia tree. This invents described Chinese cassia treeFor the water extract of Chinese cassia tree medicinal powder or Chinese cassia tree; The composition of cinnamon water extract is water-soluble polyphenol, saponin(e and a small amount of tanninAnd alkaloid, wherein water-soluble polyphenol content is 10-99%. The preparation method of the water extract of Chinese cassia tree obtains Chinese cassia tree boilingTo water extraction liquid, concentrated, with organic solvent extraction, extract concentrate drying obtains water extract; Or organic solvent extraction liquid is concentratedCarry out chromatography with silicagel column, wash-out, the dry water extract that obtains. The method that this invention has adopted water to decoct, extracts temperature higher,Polyphenol components is comparatively responsive to temperature, easily under hot conditions, is damaged.
Application number is 200810044451.X, and name is called the patent application of " method of preparing antidiabetic agent preparation taking Chinese cassia tree as raw material "Relate to the method for preparing antidiabetic agent preparation taking Chinese cassia tree as raw material. Main points are to extract with ester soluble solvent after Chinese cassia tree is pulverized, or meatThe broken rear water of cassia lignea flour or water-ethanol solution boil and extract with ester soluble solvent, and toxic component safrole is dissolved in ester soluble solventIn, the powdered substance that the extraction solution after the Chinese cassia tree powder after extraction or extraction obtains through distillation is antidiabetic agent preparation. ShouldThe leaching process of invention comprises that ester soluble solvent extracts or the step of extraction, and security is low, is unfavorable for industrial production.
In Chinese cassia tree, contain the harmful components such as cumarin, cinnaldehydrum. Cumarin has hepatotoxicity wind agitation, and median lethal dose Oral Administration in Rats is293mg/kg, mouse is oral is 196mg/kg. Low dose of cinnaldehydrum can cause that mouse movement suppresses, and ptosis, draws in a large numberPlay strong spasm, ataxia, ear blood vessel dilatation, cinclisis, righting reflex loss, death. Outward appearance behaves with composure, butStill responsive to sound and haptic stimulus reaction. Therefore in the time that Chinese cassia tree is developed as to anti-diabetic goods, must reduce wherein as far as possibleThe content of the composition such as cumarin, cinnaldehydrum is comparatively safe while making it be controlled at long-term use.
The purifying of Chinese medicine extract adopts macroporous absorbent resin more in recent years, the porous network structure of macroreticular resin, huge surfaceAmass and make it have good absorption property. Result of study shows, at refining, Effective Component of Chinese Medicine and the active component of natural drugSeparation, purifying, the aspects such as the quality control of Chinese patent drug, macroporous absorbent resin has demonstrated unique Action advantage. Chinese medicine is carriedGet liquid refining thing of obtaining after macroporous absorbent resin is processed and can make effective component highly enriched, impurity significantly reduces; Meanwhile,Resin treatment can be removed the compositions such as a large amount of carbohydrates in aqueous extract, inorganic salts, lymphatic temperament effectively, reduces the moisture absorption of product,Significantly reduce dose, the stability and the quality control that are conducive to improve product, be conducive to make the Chinese medicine of modern formulation.
Macroporous absorbent resin is by the difference of the difference of its polarity size and selected monomer molecule structure, can be divided into nonpolar, inIsopolarity, polarity and strong polarity Four types. 1) nonpolar adsorption resin: be styrene-divinylbenzene polymer, also claim virtueFragrant family adsorbent. This type of polymeric adsorbent kind is more, as DaionHP-20, D101, HPD100 etc.; 2) middle polarity is inhaledAttached resin: be polyacrylate polymer, using the methacrylate of function groups as crosslinking agent, also claim aliphatic absorptionAgent, AmberliteXAD-6, AmberliteXAD-7, AmberliteXAD-8 etc.; 3) Polar Adsorbent Resin: sulfur-bearingThe group such as oxygen, acid amides, as AmberliteXAD-9, AmberliteXAD-10, NKA-9 etc.; 4) strong Polar Adsorbent Resin:Containing amino, pyridine, nitrogen oxide isopolarity group, as AmberliteXAD-11, AmberliteXAD-12 etc.
Macroporous absorbent resin reaches separating-purifying object by the sieving actoion in physical absorption and the netted hole of resin. Macroporous absorption treeThe netted opening structure that fat comprises many microcosmic bead compositions, has huge specific area; Add that macroreticular resin is in building-up processThe polar group of middle introducing, makes it demonstrate good characterization of adsorption. The absorption affinity of macroreticular resin is due to Van der Waals force or generationThe result of hydrogen bond. Wherein, Van der Waals force is a kind of intermolecular force, comprises directive force, dispersion force, induction force etc. Due toThe existence in the netted hole of macroreticular resin porous, makes its material to different molecular size have certain screening effect. Therefore,Different organic compounds is difference in size from the different and molecular weight of the absorption affinity of macroreticular resin according to it, in the absorption of resinMechanism separates with realizing under the effect of screening principle.
At present, it is few that disclosed data is studied in the separation and purification of Chinese cassia tree polyphenol components, is not applicable to the data of suitability for industrialized production.
Summary of the invention
The object of this invention is to provide a kind of Chinese cassia tree total polyphenols extract of high-efficiency low-toxicity.
Another object of the present invention is to provide the preparation method of above-mentioned Chinese cassia tree total polyphenols extract.
A further object of the invention is to provide above-mentioned Chinese cassia tree total polyphenols extract in preparation treatment, prevention diabetes and complication thereofMedicine or be prepared with and help fall the application in hypoglycemic health products.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of Chinese cassia tree total polyphenols extract, it is the total polyphenols extracting from Chinese traditional medicine cinnamon, by weight percentage Determination of PolyphenolsHigher than 50%, impurity cumarin content is lower than 0.5%, and Determination of Cinnamaldehyde is lower than 5%;
And this Chinese cassia tree total polyphenols extract is that the method by comprising the following steps is carried out:
(1) after Chinese cassia tree is pulverized, extracts at least one times with 10%～95% ethanolic solution, merging extract, concentrates extractTo almost without alcohol taste, stir with the water dilution of 0.5 times of concentrate～4 times of amounts, leave standstill, remove sediment, obtain Chinese cassia tree thickExtract;
(2) the Chinese cassia tree crude extract of (1) gained is added on to large pore resin absorption column, first water or not higher than 15% low concentration secondAlcohol wash-out, is discarded water or low-concentration ethanol eluent, except the large impurity of depolarization, then uses 20%～80% ethanolic solutionWash-out, collects eluent, reclaims ethanol concentrated, dry, obtains Chinese cassia tree total polyphenols extract.
In Chinese cassia tree total polyphenols extract of the present invention, Determination of Polyphenols is preferably higher than 60% by weight percentage, impurity cumarinContent is preferably lower than 0.1%, and Determination of Cinnamaldehyde is preferably lower than 2.5%.
Assay method: the mensuration of total polyphenols adopts Folin-Ciocalteus method; The mensuration of cumarin and cinnaldehydrum adopts high efficiency liquid phaseMethod, chromatographic column: HypersilC18 (4.6mm × 250mm, 5 μ m), mobile phase: acetonitrile-0.1% phosphoric acid (28:72), flow velocity:1mL·min-1, measure wavelength: 280nm, column temperature: 30 DEG C.
In the preparation method of Chinese cassia tree polyphenol extract of the present invention, the method with ethanolic solution extracts described in (1) step comprises temperatureSoak that method, percolation, Microwave-assisted Extraction are followed the example of, supersonic oscillations extraction method, extract temperature and be no more than 80 DEG C.
In the preparation method of Chinese cassia tree polyphenol extract of the present invention, the macroporous absorbent resin described in (2) step be in low polarity largeMacroporous adsorbent resin, comprises HPD-100C, D-101, AB-8, DM130, X-5, D4020, HP20 etc.
Being applied as of above-mentioned Chinese cassia tree total polyphenols extract: preparation is treated, prevented the medicine of diabetes and complication thereof or contribute toFall hypoglycemic health products, wherein contain the above-mentioned Chinese cassia tree total polyphenols extract of effective dose.
Described diabetes and complication thereof comprise: type i diabetes, type ii diabetes, diabetic complication comprise eyeground pathological changes,Diabetic nephropathy, infection, DPN and cardiovascular and cerebrovascular diseases.
Described pharmaceutical dosage form comprises: oral tablet, oral disnitegration tablet, lozenge, dispersing tablet, hard capsule, soft capsule,Sustained release preparation, granule, pill, dripping pill, oral liquid, dry suspensoid agent.
Preparation method's of the present invention major advantage is:
The one, select hydrophilic solvent to extract, the recovery rate of polyphenol components is very high, and the recovery rate of oil-soluble impurities is lower,In the time adopting macroporous absorbent resin to carry out purifying to extract, reduce the extent of damage to macroporous absorbent resin, extend macroporeThe service life of polymeric adsorbent.
The 2nd, because polyphenol components is unstable, easily oxidized, especially more easily oxidized under hot conditions, the present invention adoptsExtracting method at room temperature can complete, and controls to extract that temperature is the highest is no more than 70 DEG C, and condition is gentleer, neither affects polyphenolThe extraction effect of constituents, is conducive to again the stable existence of polyphenol components.
The 3rd, adopt nonpolar or low pole macroporous absorbent resin to carry out purifying, effectively improve the content of Chinese cassia tree total polyphenols, significantlyDegree has reduced the content of the impurity such as cumarin, cinnaldehydrum, while like this Chinese cassia tree total polyphenols extract being prepared as to anti-diabetic goods,Its security is guaranteed.
Compared with prior art, in Chinese cassia tree total polyphenols extract of the present invention, the content of total polyphenols is high, and impurity is few, is preparing patent medicineWhen product or health products, can reduce dose, also can reduce to a certain extent toxic and side effect, be conducive to the security of product and haveEffect property.
Detailed description of the invention
The present invention is further elaborated by following examples, but scope of the present invention is not limited to these embodiment. So,Under method prerequisite of the present invention, simple modifications of the present invention is all belonged to the scope of protection of present invention.
Embodiment 1
Adopt the temperature method of soaking to extract, macroporous absorbent resin HPD-100C purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 500g pulverizing, taking 50% ethanol water as solvent, under 50 DEG C of conditions, extract three times, ethanolic solution is usedAmount is respectively 5L, 4L and 3L, and extraction time is respectively 3 hours, 3 hours and 2 hours, filters to merge to obtain extract.Extract is evaporated to almost without alcohol taste, adds the water dilution of 1 times of amount of concentrate, stir as suspension, leave standstill, removeAfter sediment, adsorb with macroporous absorbent resin HPD-100C, first use the water elution of 2 times of amount of resin (V/W) except depolarizationImpurity, then use the ethanol of 5 times of amount of resin (V/W) 50% to carry out wash-out, collect eluent, reduced pressure concentration reclaims ethanol, trueEmpty dry, obtain Chinese cassia tree total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight is 31.93g, and polyphenol content is 64.22%, and cumarin content is 0.05%, and cinnaldehydrum containsAmount is 1.52%.
Embodiment 2
Adopt the temperature method of soaking to extract, macroporous absorbent resin D101 purification Chinese cassia tree total polyphenols extract:
Get after two parts of pulverizing of Chinese cassia tree 500g, add 60% ethanolic solution under 40 DEG C (1), 60 DEG C of (2) conditions, to extract three times respectively,Ethanolic solution consumption is respectively 5L, 4L and 3L, extraction time is respectively 3 hours, 3 hours and 2 hours, filters and mergesTo extract. Extract is evaporated to almost without alcohol taste, adds the water dilution of 2 times of amounts of concentrate, stir as suspension,Leave standstill, remove after sediment and adsorb with macroporous absorbent resin D101, first use the water elution of 2 times of amount of resin (V/W) to removeThe impurity that depolarization is large, then use the ethanol of 4 times of amount of resin (V/W) 60% to carry out wash-out, collect eluent, reduced pressure concentration returnsReceive ethanol, vacuum drying, obtains Chinese cassia tree total polyphenols extract.
Result is as follows:
(1) Chinese cassia tree total polyphenols extract gross weight 31.0g, polyphenol content is 62.52%, cumarin content is 0.06%, cinnaldehydrumContent is 1.83%.
(2) Chinese cassia tree total polyphenols extract gross weight 32.5g, polyphenol content is 61.33%, cumarin content is 0.05%, cinnaldehydrumContent is 1.75%.
Embodiment 3
Employing percolation extracts, macroporous absorbent resin D4020 purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 1kg pulverizing, add 50% ethanolic solution 2L to mix thoroughly, airtight placement, after 20 minutes, packs in percolator,Add again 50% ethanolic solution of 6L, place 24 hours, make to carry out diacolation after the abundant diffusion of solvent, collect percolate.Percolate is evaporated to almost without alcohol taste, adds the water dilution of 1.5 times of amounts of concentrate, stir as suspension, leave standstill, removeGo to adsorb with macroporous absorbent resin D4020 after sediment, first use 10% ethanolic solution of 1.5 times of amount of resin (V/W) to washRemove the impurity that depolarization is large, then use the ethanol of 5 times of amount of resin (V/W) 50% to carry out wash-out, collect eluent, reduce pressure denseRetract and receive ethanol, spraying is dry, obtains Chinese cassia tree total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight 62.12g, polyphenol content is 64.83%, cumarin content is 0.05%, Determination of CinnamaldehydeBe 1.96%.
Embodiment 4
Adopt the temperature method of soaking to extract, macroporous absorbent resin AB-8 purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 200g pulverizing, add 60% ethanolic solution to extract twice under 50 DEG C of conditions, ethanolic solution consumption is respectively500mL and 400mL, extraction time is respectively 4 hours and 2 hours, filters to merge to obtain extract. Extract is reduced pressure denseBe reduced to almost without alcohol taste, add the water dilution of 2 times of amounts of concentrate, stir as suspension, leave standstill, use macropore after removing sedimentPolymeric adsorbent D101 adsorbs, and first uses the water elution of 2 times of amount of resin (V/W) except the large impurity of depolarization, then uses 4 timesThe ethanol of amount of resin (V/W) 70% carries out wash-out, collects eluent, and reduced pressure concentration reclaims ethanol, and spraying is dry, obtains meatOsmanthus total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight 10.31g, polyphenol content is 62.52%, cumarin content is 0.06%, Determination of CinnamaldehydeBe 1.83%.
Embodiment 5
Adopt the temperature method of soaking to extract, macroporous absorbent resin DM130 purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 1kg pulverizing, add 30% ethanolic solution, extract three times at 50 DEG C, each ethanolic solution consumption is 8000ml,Each extraction 3 hours, filters merging and obtains extract. Extract is evaporated to almost without alcohol taste, adds concentrate 1.5Doubly the water of amount stirs as suspension, leaves standstill, and removes after sediment and adsorbs with macroporous absorbent resin DM130, first uses 1.5 timesAmount of resin (V/W) 15% ethanolic solution washes away polar impurity, then uses the ethanol of 8 times of amount of resin (V/W) 40% to carry out wash-out,Collect eluent, reduced pressure concentration reclaims ethanol, and spraying is dry, obtains Chinese cassia tree total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight 62.4g, polyphenol content is 63.57%, cumarin content is 0.06%, Determination of CinnamaldehydeBe 1.83%.
Embodiment 6
Employing percolation extracts, macroporous adsorption resin X-5 purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 1kg pulverizing, add 70% ethanolic solution 2L to mix thoroughly, airtight placement, after 20 minutes, packs in percolator,Add again 50% ethanolic solution of 5L, place 24 hours, make to collect percolate after the abundant diffusion of solvent. Percolate is subtractedPressure is concentrated into almost without alcohol taste, with the water dilution of 3 times of amounts of concentrate, stirs as suspension, leaves standstill, and removes after sediment with largeMacroporous adsorbent resin X-5 adsorbs, and first uses the washing depolarization impurity of 2 times of amount of resin (V/W), then uses 5 times of amount of resin (V/W)50% ethanol carries out wash-out, collects eluent, and reduced pressure concentration reclaims ethanol, and vacuum drying, obtains Chinese cassia tree total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight is 61.37g, and polyphenol content is 61.57%, and cumarin content is 0.07%, and cinnaldehydrum containsAmount is 2.01%.
Embodiment 7
Employing percolation extracts, macroporous absorbent resin HP20 purification Chinese cassia tree total polyphenols extract:
Get after Chinese cassia tree 500g pulverizing, add 40% ethanolic solution 1L to mix thoroughly, airtight placement, after 20 minutes, packs in percolator,Add again 40% ethanolic solution of 3L, place 24 hours, make to collect percolate after the abundant diffusion of solvent. Percolate is subtractedPressure is concentrated into almost without alcohol taste, with the water dilution of 2 times of amounts of concentrate, stirs as suspension, leaves standstill, and removes after sediment with largeMacroporous adsorbent resin X-5 adsorbs, and first uses the washing depolarization impurity of 2 times of amount of resin (V/W), then uses 4 times of amount of resin (V/W)60% ethanol carries out wash-out, collects eluent, and reduced pressure concentration reclaims ethanol, and vacuum drying, obtains Chinese cassia tree total polyphenols extract.
Result is as follows:
Chinese cassia tree total polyphenols extract gross weight is 30.26g, and polyphenol content is 62.96%, and cumarin content is 0.06%, and cinnaldehydrum containsAmount is 1.97%.
Embodiment 8
The preparation of the tablet medicine that contains Chinese cassia tree total polyphenols extract:
Get the Chinese cassia tree total polyphenols extract 30g that embodiment 1 obtains, pregelatinized starch 10g, microcrystalline cellulose 10g, mixes allEven, water is granulated, and sieves, dry, then adds dolomol 0.25g, and total mixed rear compressing tablet, obtains containing Chinese cassia tree total polyphenols extractTablet medicine.
Embodiment 9
The granules medicine preparation that contains Chinese cassia tree total polyphenols extract:
Get the Chinese cassia tree total polyphenols extract 30g that in embodiment 2, (1) obtains, dextrin 20g, sweet mellow wine 50g, mixes,Use 30% alcohol granulation, sieve, dry, make the granules medicine containing Chinese cassia tree total polyphenols extract.
Embodiment 10
The hard capsule medicine preparation that contains Chinese cassia tree total polyphenols extract:
Get the Chinese cassia tree total polyphenols extract 30g that embodiment 3 obtains, talcum powder 1g, is loaded on after mixing in No. 2 capsulae vacuuses,Make the hard capsule medicine containing Chinese cassia tree total polyphenols extract.
Claims (10)
1. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage, wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt the temperature method of soaking to extract, macroporous absorbent resin HPD-100C purification Chinese cassia tree total polyphenols extract, get after Chinese cassia tree 500g pulverizing, taking 50% ethanol water as solvent, under 50 DEG C of conditions, extract three times, ethanolic solution consumption is respectively 5L, 4L and 3L, extraction time is respectively 3 hours, 3 hours and 2 hours, filter to merge and obtain extract, extract is evaporated to almost without alcohol taste, add the water dilution of 1 times of amount of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous absorbent resin HPD-100C, first remove polar impurity with volume mass than the water elution that is 2 times of amount of resin, carry out wash-out with volume mass than 50% ethanol that is 5 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, vacuum drying, obtain Chinese cassia tree total polyphenols extract.
2. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt the temperature method of soaking to extract, macroporous absorbent resin D101 purification Chinese cassia tree total polyphenols extract, get after two parts of pulverizing of Chinese cassia tree 500g, add 60% ethanolic solution respectively 40 DEG C (1), under 60 DEG C of (2) conditions, extract three times, ethanolic solution consumption is respectively 5L, 4L and 3L, extraction time is respectively 3 hours, 3 hours and 2 hours, filter to merge and obtain extract, extract is evaporated to almost without alcohol taste, add the water dilution of 2 times of amounts of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous absorbent resin D101, first remove the large impurity of depolarization with volume mass than the water elution that is 2 times of amount of resin, carry out wash-out with volume mass than 60% ethanol that is 4 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, vacuum drying, obtain Chinese cassia tree total polyphenols extract.
3. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt percolation to extract, macroporous absorbent resin D4020 purification Chinese cassia tree total polyphenols extract, get after Chinese cassia tree 1kg pulverizing, add 50% ethanolic solution 2L to mix thoroughly, airtight placement is after 20 minutes, pack in percolator, add again 50% ethanolic solution of 6L, place 24 hours, make to carry out diacolation after the abundant diffusion of solvent, collect percolate, percolate is evaporated to almost without alcohol taste, add the water dilution of 1.5 times of amounts of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous absorbent resin D4020, first remove the large impurity of depolarization with volume mass than the 10% ethanolic solution wash-out that is 1.5 times of amount of resin, carry out wash-out with volume mass than 50% ethanol that is 5 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, spraying is dry, obtain Chinese cassia tree total polyphenols extract.
4. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt the temperature method of soaking to extract, macroporous absorbent resin AB-8 purification Chinese cassia tree total polyphenols extract, get after Chinese cassia tree 200g pulverizing, add 60% ethanolic solution to extract twice under 50 DEG C of conditions, ethanolic solution consumption is respectively 500mL and 400mL, extraction time is respectively 4 hours and 2 hours, filter to merge and obtain extract, extract is evaporated to almost without alcohol taste, add the water dilution of 2 times of amounts of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous absorbent resin D101, first remove the large impurity of depolarization with volume mass than the water elution that is 2 times of amount of resin, carry out wash-out with volume mass than 70% ethanol that is 4 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, spraying is dry, obtain Chinese cassia tree total polyphenols extract.
5. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt the temperature method of soaking to extract, macroporous absorbent resin DM130 purification Chinese cassia tree total polyphenols extract, get after Chinese cassia tree 1kg pulverizing, add 30% ethanolic solution, at 50 DEG C, extract three times, each ethanolic solution consumption is 8000ml, each extraction 3 hours, filter to merge and obtain extract, extract is evaporated to almost without alcohol taste, add the water of 1.5 times of amounts of concentrate to stir as suspension, leave standstill, remove after sediment and adsorb with macroporous absorbent resin DM130, first wash away polar impurity with volume mass than 15% ethanolic solution that is 1.5 times of amount of resin, carry out wash-out with volume mass than 40% ethanol that is 8 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, spraying is dry, obtain Chinese cassia tree total polyphenols extract.
6. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt percolation to extract, macroporous adsorption resin X-5 purification Chinese cassia tree total polyphenols extract: get after Chinese cassia tree 1kg pulverizing, add 70% ethanolic solution 2L to mix thoroughly, airtight placement is after 20 minutes, pack in percolator, add again 50% ethanolic solution of 5L, place 24 hours, make to collect percolate after the abundant diffusion of solvent, percolate is evaporated to almost without alcohol taste, with the water dilution of 3 times of amounts of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous adsorption resin X-5, first with volume mass than the washing depolarization impurity that is 2 times of amount of resin, carry out wash-out with volume mass than 50% ethanol that is 5 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, vacuum drying, obtain Chinese cassia tree total polyphenols extract.
7. a Chinese cassia tree total polyphenols extract, it is characterized in that by weight percentage wherein Determination of Polyphenols is higher than 50%, impurity cumarin content is lower than 0.5%, Determination of Cinnamaldehyde is lower than 5%, preparation method is as follows: adopt percolation to extract, macroporous absorbent resin HP20 purification Chinese cassia tree total polyphenols extract, get after Chinese cassia tree 500g pulverizing, add 40% ethanolic solution 1L to mix thoroughly, airtight placement is after 20 minutes, pack in percolator, add again 40% ethanolic solution of 3L, place 24 hours, make to collect percolate after the abundant diffusion of solvent, percolate is evaporated to almost without alcohol taste, with the water dilution of 2 times of amounts of concentrate, stir as suspension, leave standstill, remove after sediment and adsorb with macroporous adsorption resin X-5, first with volume mass than the washing depolarization impurity that is 2 times of amount of resin, carry out wash-out with volume mass than 60% ethanol that is 4 times of amount of resin again, collect eluent, reduced pressure concentration reclaims ethanol, vacuum drying, obtain Chinese cassia tree total polyphenols extract.
8. according to the Chinese cassia tree total polyphenols extract described in claim 1-7 any one, it is characterized in that in described Chinese cassia tree total polyphenols extract, Determination of Polyphenols is higher than 60% by weight percentage, impurity cumarin content is lower than 0.1%, and Determination of Cinnamaldehyde is lower than 2.5%.
9. according to the application of the Chinese cassia tree total polyphenols extract described in claim 1-7 any one, it is characterized in that the application of Chinese cassia tree total polyphenols extract in the medicine of preparation treatment, prevention diabetic complication.
10. the application in the medicine of preparation treatment, prevention diabetic complication according to the Chinese cassia tree total polyphenols extract described in claim 1-7 any one, is characterized in that described pharmaceutical dosage form comprises: oral tablet, capsule, granule, pill, oral liquid, dry suspensoid agent.
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