CN103265583B - A kind of preparation method of stachyose crystal - Google Patents
A kind of preparation method of stachyose crystal Download PDFInfo
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- CN103265583B CN103265583B CN201310068090.3A CN201310068090A CN103265583B CN 103265583 B CN103265583 B CN 103265583B CN 201310068090 A CN201310068090 A CN 201310068090A CN 103265583 B CN103265583 B CN 103265583B
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Abstract
The invention discloses a kind of preparation method of stachyose crystal, the method comprises the steps: (1), and liquid glucose is just prepared: cleaned by raw material, squeeze the juice after hot water extraction to obtain liquid glucose, liquid glucose is evaporated to refractive power 30 ~ 50 ° of Brix, in liquid glucose: ethanol (V/V)=1:(2-6) ratio add 95% ethanol, stir, leave standstill back cardboard to filter, filter residue 75% washing with alcohol, filtrate mixes, at 45 ~ 75 DEG C of vacuum concentration to 70 ~ 80 ° Bix, obtain the molten part medicinal extract of alcohol; (2) coarse crystallization; (3) liquid glucose is prepared again; (4) recrystallization; (5) vacuum-drying, obtain a kind of stachyose purity higher than 99.0% stachyose crystal.
Description
Technical field
The present invention relates to a kind of preparation method of stachyose crystal, particularly relating to a kind of take natural phant as the method that high purity stachyose crystallization prepared by raw material.
Background technology
Stachyose is extensively present in leguminous plants, labiate and goatweed, is a kind of natural functional oligose, can profitable strain (bifidus bacillus and Bacterium lacticum etc.) in Effective multiplication enteron aisle, has good physiological function.Along with reaching its maturity and the Normalization in stachyose market of stachyose production extractive technique, the preparation of high purity stachyose crystallization will be studied for stachyose basic test and Check and Inspection means lay a good foundation.
Look into patent " preparation method of high purity water threose " (patent No. 200910083903.X) and adopt the plant such as Rhizome of Bear's-foot Fern, silver bar to be raw material, application of fermentation legal system is for high purity stachyose, and stachyose purity reaches more than 85%.Patent " prepares the method for high purity water threose ", and (patent No. 200910085212.3) adopts the plant such as Rhizome of Bear's-foot Fern, silver bar to be raw material with industrial chromatographic separation technology, and application chromatography prepares high purity stachyose, and stachyose purity reaches more than 90%.Patent " stachyose and its production and use " (number of patent application 201210039455.5) adopts the red sage root to be raw material separation and Extraction stachyose, and stachyose purity is more than 80%.Above-mentioned patent is all different from stachyose purifying technique of the present invention, and purity is also different.
Patent " crystallization of carbohydrate " (patent No. 03818058.8) relate to a kind of by nanofiltration, hydrolysis and/or chromatography from containing one or more reducing sugars solution remove crystallization inhibitor, reducing sugar is selected from fructose and wood sugar.Different from crystallization processes of the present invention, raw material and final product are also different.
Be raw material with glutinous rehmannia in paper " preparation and osmanthus attached glutinous rehmannia side's polysaccharide quality control method of stachyose reference material are studied ", have employed the column chromatography means such as macroporous adsorbent resin, gac, silica gel, ODS, obtain the stachyose reference material that purity is about 99.8%.Different from purification means of the present invention, products therefrom state is also different.
In sum, the present invention adopts natural phant silver bar, Rhizome of Bear's-foot Fern, the red sage root, glutinous rehmannia etc. for raw material, adopt water extraction in conjunction with alcohol extracting method carry out recrystallization obtain stachyose purity higher than 99.0% crystal, the preparation for stachyose reference material provides environmental protection thinking easily.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of preparation method of stachyose crystal is provided.
Technical scheme of the present invention is summarized as follows:
A preparation method for stachyose crystal, is made up of following steps:
(1) liquid glucose is just prepared: select the raw material being rich in stachyose to comprise the plants such as fresh silver bar, Rhizome of Bear's-foot Fern, the red sage root, glutinous rehmannia, raw material is cleaned, squeeze the juice after hot water extraction and get liquid glucose, liquid glucose is evaporated to refractive power 30 ~ 50 ° of Brix, in liquid glucose: ethanol (V/V)=1:(2-6) ratio add 95% ethanol, stir, leave standstill back cardboard to filter, filter residue 75% washing with alcohol, filtrate mixes, at 45 ~ 75 DEG C of vacuum concentration to 70 ~ 80 ° Bix, obtain the molten part medicinal extract of alcohol;
(2) coarse crystallization: medicinal extract is poured in insulating container, nature slow cooling, after 1-10 days, crystalline particulate is had to occur in medicinal extract inside, and prolongation crystal becomes large gradually in time, adopt 20-80% ethanolic soln cleaning medicinal extract, 30-50 DEG C of vacuum-drying, obtain stachyose purity higher than the coarse crystallization of 90%, collect ethanol filtrate;
(3) liquid glucose is prepared again: step (2) gained stachyose coarse crystallization or commercially available stachyose product (stachyose purity >=80%) are dissolved, be mixed with the liquid glucose of 10-30 ° of Bix, add 0.5-1.5% activated carbon decolorizing, be heated to 80 ~ 90 DEG C, stir 10-30min, cardboard filter, becomes the liquid glucose of refractive power 40-70 ° Brix at 45 ~ 75 DEG C of vacuum concentration by filtrate;
(4) recrystallization: in step (3) gained liquid glucose: ethanol (V/V)=1:(0.5-2) ratio add the ethanol of 70-95% purity, solution become turbid post-heating be stirred to 65 DEG C keep 5-20min, place in insulating container and keep slow cooling, leave standstill and deposit, transparent diamond crystal particle is there is after 1-7 days, adopt 30-70% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning, mix concentrated with ethanol filtrate in step (2), reclaim ethanol, concentrated liquid glucose is for the preparation of ordinary purity stachyose product;
(5) crystalline particle after cleaning is carried out vacuum-drying at 30-50 DEG C by vacuum-drying, obtains stachyose recrystallization.In crystallization, stachyose content is greater than 99.0%.
Described step (1) is preferably: select the raw material being rich in stachyose to comprise the plants such as silver bar, Rhizome of Bear's-foot Fern, the red sage root, glutinous rehmannia, raw material is cleaned, squeeze the juice after hot water extraction and get liquid glucose, liquid glucose is evaporated to refractive power 45 ° of Brix, in liquid glucose: the ratio of ethanol (V/V)=1:3 adds 95% ethanol, stir, leave standstill back cardboard to filter, filter residue 75% washing with alcohol, filtrate mixes, at 60 DEG C of vacuum concentration to 73 ° Bix, obtain the molten part medicinal extract of alcohol.
Described step (2) is preferably: poured into by medicinal extract in insulating container, natural slow cooling, after 4 days, there is crystalline particulate to occur in medicinal extract inside, adopt 50% ethanolic soln cleaning medicinal extract, 40 DEG C of vacuum-dryings, obtain the coarse crystallization of stachyose purity about 95%, collect ethanol filtrate.
Described step (3) is preferably: step (2) gained stachyose coarse crystallization or commercially available stachyose product (stachyose purity >=85%) are dissolved, be mixed with the liquid glucose of 20 ° of Bix, add 1% activated carbon decolorizing, be heated to 85 DEG C, stir 20min, cardboard filter, becomes the liquid glucose of refractive power 55 ° of Brix at 60 DEG C of vacuum concentration by filtrate.
Described step (4) is preferably: in step (3) gained liquid glucose: the ratio of ethanol (V/V)=1:1 adds 90% ethanol, solution become turbid post-heating be stirred to 65 DEG C keep 10min, place in insulating container and keep slow cooling, leave standstill after depositing 4 days and occur transparent diamond crystal particle, adopt 50% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning, mix concentrated with ethanol filtrate in step (2), reclaim ethanol, concentrated liquid glucose is for the preparation of ordinary purity stachyose product, and in product, stachyose purity is about 75%.
Belonging to step (5) preferably: by cleaning after crystalline particle carry out vacuum-drying at 40 DEG C, obtain stachyose recrystallization, in crystallization, stachyose content is greater than 99.0%.
Feature of the present invention:
1. the present invention adopts crystallization process from natural phant, prepare stachyose crystal, and stachyose crystal purity reaches more than 99.0%, and the Basic Experiment Study that preparation and stachyose for stachyose reference material are correlated with has laid good basis.
2. the present invention is preparing the simple environmental protection of solvent adopted in stachyose crystal technological process, can recycle and reuse.
3. the present invention take natural phant as raw material, water extraction is adopted to obtain the stachyose crystal of high purity more than 99.0% in conjunction with the technique of alcohol extracting crystallization, surplus solution also can be used to preparation purity at the ortho-water threose product of about 70%, improves the utility value of natural phant.
Accompanying drawing explanation
Accompanying drawing 1 is the HPLC figure of fresh Rhizome of Bear's-foot Fern Aqueous extracts.
Accompanying drawing 2 is the HPLC figure of fresh Rhizome of Bear's-foot Fern liquid glucose alcohol extracting coarse crystallization.
The HPLC figure collection of illustrative plates 1 that accompanying drawing 3 fresh Rhizome of Bear's-foot Fern liquid glucose alcohol extracting recrystallization and sigma stachyose standard substance contrast is that the HPLC of recrystallization schemes, and collection of illustrative plates 2 is the HPLC figure of stachyose standard substance (cas54261-98-2)
Accompanying drawing 4 is the HPLC figure of ortho-water threose product prepared by the ethanol filtrate collected.
Embodiment
The present invention adopts natural phant silver bar, Rhizome of Bear's-foot Fern, the red sage root, glutinous rehmannia etc. for raw material, adopt water extraction in conjunction with alcohol extracting method carry out recrystallization obtain stachyose purity higher than 99.0% crystal
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment 1
A preparation method for stachyose crystal, is made up of following steps:
(1) liquid glucose is just prepared: cleaned by fresh Rhizome of Bear's-foot Fern, squeeze the juice after hot water extraction and get liquid glucose, liquid glucose is evaporated to refractive power 45 ° of Brix, in liquid glucose: the ratio of ethanol (V/V)=1:2 adds 95% ethanol, stirs, leave standstill back cardboard and filter, filter residue 75% washing with alcohol, filtrate mixes, and at 55 DEG C of vacuum concentration to 75 ° Bix, obtains the molten part medicinal extract of alcohol;
(2) coarse crystallization: medicinal extract is poured in insulating container, nature slow cooling, after 7 days, have crystalline particulate to occur in medicinal extract inside, and prolongation crystal becomes large gradually in time, adopt 50% ethanolic soln cleaning medicinal extract, 40 DEG C of vacuum-dryings, obtain stachyose coarse crystallization, purity is 95.1%, collects ethanol filtrate;
(3) liquid glucose is prepared again: step (2) gained stachyose coarse crystallization dissolved, be mixed with the liquid glucose of 20 ° of Bix, add 1.5% activated carbon decolorizing, be heated to 85 DEG C, and stir 15min, cardboard filter, becomes the liquid glucose of refractive power 55 ° of Brix at 60 DEG C of vacuum concentration by filtrate;
(4) recrystallization: in step (3) gained liquid glucose: the ratio of ethanol (V/V)=1:1 adds the ethanol of 90% purity, solution become turbid post-heating be stirred to 65 DEG C keep 10min, place in insulating container and keep slow cooling, leave standstill and deposit, transparent diamond crystal particle is there is after 5 days, adopt 40% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning, mix concentrated with ethanol filtrate in step (2), reclaim ethanol, ordinary purity stachyose product prepared by concentrated liquid glucose, and product purity is 75.2%;
(5) crystalline particle after cleaning is carried out vacuum-drying at 40 DEG C by vacuum-drying, obtains stachyose recrystallization.In crystallization, stachyose purity is 99.8%.
Embodiment 2
A preparation method for stachyose crystal, is made up of following steps:
(1) liquid glucose is just prepared: cleaned by new Rehmannia Root, squeeze the juice after hot water extraction and get liquid glucose, liquid glucose is evaporated to refractive power 40 ° of Brix, in liquid glucose: the ratio of ethanol (V/V)=1:3 adds 95% ethanol, stirs, leave standstill back cardboard and filter, filter residue 75% washing with alcohol, filtrate mixes, and at 75 DEG C of vacuum concentration to 80 ° Bix, obtains the molten part medicinal extract of alcohol;
(2) coarse crystallization: medicinal extract is poured in insulating container, natural slow cooling, after 3 days, have crystalline particulate to occur in medicinal extract inside, and prolongation crystal becomes large gradually in time, adopt 70% ethanolic soln cleaning medicinal extract, 50 DEG C of vacuum-dryings, obtaining stachyose purity in coarse crystallization is 92.2%;
(3) liquid glucose is prepared again: step (2) gained stachyose coarse crystallization dissolved, be mixed with the liquid glucose of 30 ° of Bix, add 1% activated carbon decolorizing, be heated to 90 DEG C, and stir 30min, cardboard filter, becomes the liquid glucose of refractive power 65 ° of Brix at 75 DEG C of vacuum concentration by filtrate;
(4) recrystallization: in step (3) gained liquid glucose: the ratio of ethanol (V/V)=1:0.8 adds the ethanol of 95% purity, solution become turbid post-heating be stirred to 65 DEG C keep 20min, place in insulating container and keep slow cooling, leave standstill and deposit, transparent diamond crystal particle is there is after 2 days, adopt 50% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning, mix concentrated with ethanol filtrate in step (2), reclaim ethanol, ordinary purity stachyose product prepared by concentrated liquid glucose, and product purity is 72%;
(5) crystalline particle after cleaning is carried out vacuum-drying at 50 DEG C by vacuum-drying, obtains stachyose recrystallization.In crystallization, stachyose is 99.5%.
Experimental example 3
A preparation method for stachyose crystal, is made up of following steps:
(1) liquid glucose preparation: commercially available stachyose product (stachyose purity is 86%) is dissolved, is mixed with the liquid glucose of 10 ° of Bix, adds 0.5% activated carbon decolorizing, be heated to 80 DEG C, stir 15min, cardboard filter, becomes the liquid glucose of refractive power 30 ° of Brix at 45 DEG C of vacuum concentration by filtrate;
(2) recrystallization: by gained liquid glucose: the ratio of ethanol (V/V)=1:2 adds the ethanol of 90% purity, solution become turbid post-heating be stirred to 65 DEG C keep 5min, place in insulating container and keep slow cooling, leave standstill and deposit, after 7 days, occur transparent diamond crystal particle, adopt 60% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning to concentrate, reclaim ethanol, the stachyose product purity that concentrated filtrate is prepared is 73%;
(3) vacuum-drying: the crystalline particle after cleaning is carried out vacuum-drying at 30 DEG C, obtains stachyose crystal.In crystallization, stachyose content is 99.1%.
Claims (1)
1. the preparation method of a stachyose crystal, it is characterized in that, be made up of following steps: (1) liquid glucose is just prepared: raw material be selected from be rich in stachyose fresh silver bar, Rhizome of Bear's-foot Fern, the red sage root or glutinous rehmannia, raw material is cleaned, squeeze the juice after hot water extraction and get liquid glucose, liquid glucose is evaporated to refractive power 30 ~ 50 ° of Brix, in liquid glucose: ethanol (V/V)=1:(2-6) ratio add 95% ethanol, stir, leave standstill back cardboard to filter, filter residue 75% washing with alcohol, filtrate mixes, at 45 ~ 75 DEG C of vacuum concentration to 70 ~ 80 ° Brix, obtain the molten part medicinal extract of alcohol; (2) coarse crystallization: medicinal extract is poured in insulating container, nature slow cooling, after 1-10 days, crystalline particulate is had to occur in medicinal extract inside, and prolongation crystal becomes large gradually in time, adopt 20-80% ethanolic soln cleaning medicinal extract, 30-50 DEG C of vacuum-drying, obtain stachyose purity higher than the coarse crystallization of 90%, collect ethanol filtrate; (3) liquid glucose is prepared again: dissolved by the commercially available stachyose product of step (2) gained stachyose coarse crystallization or stachyose purity >=80%, be mixed with the liquid glucose that refractive power is 10-30 ° of Brix, add 0.5-1.5% activated carbon decolorizing, be heated to 80 ~ 90 DEG C, stir 10-30min, cardboard filter, becomes the liquid glucose of refractive power 40-70 ° Brix at 45 ~ 75 DEG C of vacuum concentration by filtrate; (4) recrystallization: in step (3) gained liquid glucose: ethanol (V/V)=1:(0.5-2) ratio add the ethanol of 70-95% purity, solution become turbid post-heating be stirred to 65 DEG C keep 5-20min, place in insulating container and keep slow cooling, leave standstill and deposit, transparent diamond crystal particle is there is after 1-7 days, adopt 30-70% ethanolic soln cleaning crystalline particle, collect the ethanol filtrate after cleaning, mix concentrated with ethanol filtrate in step (2), reclaim ethanol, concentrated liquid glucose is for the preparation of ordinary purity stachyose product; (5) vacuum-drying: the crystalline particle after cleaning is carried out vacuum-drying at 30-50 DEG C, and obtain stachyose recrystallization, in crystallization, stachyose content is greater than 99.0%.
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| CN104262418B (en) * | 2014-09-26 | 2017-04-19 | 何龙 | Production method of crystallized water threose |
| CN104621436B (en) * | 2014-10-10 | 2017-12-15 | 浙江玛卡人生生物工程研究所 | A kind of Maca extract removes sugared technique |
| CN105017339B (en) * | 2015-07-01 | 2018-03-09 | 浙江大学 | A kind of method that SMBC separation prepares raffinose and stachyose |
| CN106632527A (en) * | 2016-12-19 | 2017-05-10 | 西安源森生物科技有限公司 | Stachyose preparation method |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1223265A (en) * | 1998-01-12 | 1999-07-21 | 济南三株药业有限公司 | Process for preparing stachyose and its products and application |
| CN1300857A (en) * | 1999-12-23 | 2001-06-27 | 中国食品发酵工业研究所 | Stachyose and its preparing process |
| CN1317492A (en) * | 2001-03-20 | 2001-10-17 | 黄京晨 | Process for preparing bifidobacterium factor oligose from gestumor tuber |
| CN1339443A (en) * | 2000-08-25 | 2002-03-13 | 陈梅英 | Extracting method for stachyose |
| CN1473835A (en) * | 2001-08-13 | 2004-02-11 | 西安大鹏生物科技股份有限公司 | Process for extracting high purity stachyose from stachys sieboldii |
| CN1699388A (en) * | 2005-05-23 | 2005-11-23 | 青岛国风药业股份有限公司 | Raffinoses oligosaccharide with stachyose as main materials and method for preparing same |
| CN1990494A (en) * | 2005-12-30 | 2007-07-04 | 上海中医药大学附属曙光医院 | Dried rehmannia root oligosaccharide and its preparation method and uses |
| CN101077880A (en) * | 2006-05-26 | 2007-11-28 | 厦门牡丹香化实业有限公司 | Method for extracting stachyose from euroleon sinicus |
| CN101139370A (en) * | 2007-09-07 | 2008-03-12 | 凤县沃普森生物科技有限公司 | Method for extracting stachyose |
| CN101597635A (en) * | 2009-05-12 | 2009-12-09 | 中国食品发酵工业研究院 | The preparation method of high purity stachyose |
| CN101633676A (en) * | 2009-06-04 | 2010-01-27 | 中国食品发酵工业研究院 | Method for preparing high-purity stachyose by using plant chromatographic separation technology |
| CN101717415A (en) * | 2009-12-24 | 2010-06-02 | 江南大学 | Method for efficiently preparing stachyose from stachys sieboldii |
| CN102229627A (en) * | 2011-05-13 | 2011-11-02 | 南京中医药大学 | Method for preparing stachyose by using water-extraction alcohol-precipitation of salvia miltiorrhiza |
| CN102558249A (en) * | 2012-02-21 | 2012-07-11 | 复旦大学 | Salvia miltiorrhiza stachyose, and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3623974B2 (en) * | 1993-09-13 | 2005-02-23 | カルピス株式会社 | Oligosaccharide purification method |
-
2013
- 2013-03-05 CN CN201310068090.3A patent/CN103265583B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1223265A (en) * | 1998-01-12 | 1999-07-21 | 济南三株药业有限公司 | Process for preparing stachyose and its products and application |
| CN1300857A (en) * | 1999-12-23 | 2001-06-27 | 中国食品发酵工业研究所 | Stachyose and its preparing process |
| CN1339443A (en) * | 2000-08-25 | 2002-03-13 | 陈梅英 | Extracting method for stachyose |
| CN1317492A (en) * | 2001-03-20 | 2001-10-17 | 黄京晨 | Process for preparing bifidobacterium factor oligose from gestumor tuber |
| CN1473835A (en) * | 2001-08-13 | 2004-02-11 | 西安大鹏生物科技股份有限公司 | Process for extracting high purity stachyose from stachys sieboldii |
| CN1699388A (en) * | 2005-05-23 | 2005-11-23 | 青岛国风药业股份有限公司 | Raffinoses oligosaccharide with stachyose as main materials and method for preparing same |
| CN1990494A (en) * | 2005-12-30 | 2007-07-04 | 上海中医药大学附属曙光医院 | Dried rehmannia root oligosaccharide and its preparation method and uses |
| CN101077880A (en) * | 2006-05-26 | 2007-11-28 | 厦门牡丹香化实业有限公司 | Method for extracting stachyose from euroleon sinicus |
| CN101139370A (en) * | 2007-09-07 | 2008-03-12 | 凤县沃普森生物科技有限公司 | Method for extracting stachyose |
| CN101597635A (en) * | 2009-05-12 | 2009-12-09 | 中国食品发酵工业研究院 | The preparation method of high purity stachyose |
| CN101633676A (en) * | 2009-06-04 | 2010-01-27 | 中国食品发酵工业研究院 | Method for preparing high-purity stachyose by using plant chromatographic separation technology |
| CN101717415A (en) * | 2009-12-24 | 2010-06-02 | 江南大学 | Method for efficiently preparing stachyose from stachys sieboldii |
| CN102229627A (en) * | 2011-05-13 | 2011-11-02 | 南京中医药大学 | Method for preparing stachyose by using water-extraction alcohol-precipitation of salvia miltiorrhiza |
| CN102558249A (en) * | 2012-02-21 | 2012-07-11 | 复旦大学 | Salvia miltiorrhiza stachyose, and preparation method and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| Preparation of crystalline stachyose;R.S.Shallenberger,;《Chemistry & Industry》;19610415;第475页 * |
| 地黄中水苏糖的分离与脱色;樊海燕,等;《精细化工》;20081130;第25卷(第11期);第1087-1091页 * |
| 地黄水苏糖提取工艺研究;洪毅,等;《中国药业》;20090305;第18卷(第05期);第37-39页 * |
| 水苏糖的提取及初步精制工艺的研究;梁丽心;《中国食品添加剂》;20051215(第06期);第25-28页 * |
| 银条水苏糖提取条件研究;陈燕,等;《天然产物研究与开发》;20111231;第23卷(第01期);第123-130页 * |
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