CN1473835A - Process for extracting high purity stachyose from stachys sieboldii - Google Patents
Process for extracting high purity stachyose from stachys sieboldii Download PDFInfo
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Abstract
The present invention provides the extracting process of high purity, as high as 85-95 %, stachyose from plant stachys sieboldii. The said process is one clean production process, in which continuous leaching technology, membrane separating technology and ion exchange technology are used. The stachyose product is white powder and is one excellent bifidofactor capable of proliferating bifidobacteria obviously.
Description
The present invention is a kind of production method of extracting high purity stachyose from kobold, and stachyose is naturally occurring tetrose, is made up of fructose-glucose-semi-lactosi-semi-lactosi, is good bifidus factor.Main the promotion short chain fatty acid increases, and makes intestinal acidityization as breeding intestinal bifidobacteria, has the spoilage product of inhibition to form, the effect of resistance poison, toxin expelling, detoxifcation and the element absorption such as raising calcium, magnesium of inhibition cholesterol rising etc.
Human understanding to bifidus factor is the understanding that starts from bifidus bacillus, got the laboratory found bifidus bacillus from breast-feeding infant ight soil since at Bath from the Tisser of France, unremitting effort through numerous scientists, confirmed that bifidus bacillus is one of dominant bacteria among the human intestinal microflora, it also is the flora of newborn infant's enteron aisle field planting the earliest.The function of bifidus bacillus can reduce: (1) diarrhea and anti-constipation; Its physiological action is to keep good intestinal environment by the field planting of attacking bacterium or pathogenic bacteria outside stoping, thus the two-ways regulation function that realization is not only controlled diarrhoea but also prevented constipation.(2) immunomodulatory and antitumor; Full cell of bifidus bacillus or cell wall components have strengthens or promotes autoimmune attack effect to malignant cell.(3) synthesise vitamins and amino acid; But known bifidus bacillus synthesise vitamins B1, B2, B6, K, nicotinic acid, folic acid etc. have a positive effect to HUMAN HEALTH.(4) reduce cholesterol levels in the blood, prevent and treat hypertension.(5) anti-infective.
Bifidus bacillus quantity in the human intestinal is with different significant differences of age, Zong bifidus bacillus accounts for more than 90% of bacterium in the enteron aisle in the breast-feeding infant enteron aisle, the artificial feeding infant is 61%, and health adult is 14.8%, and the elderly is 3.2%.And bifidus bacillus is a kind of anerobe, not only produces difficulty, even and take active bacteria formulation, can make it to arrive enteron aisle and survivor's quantity also seldom, can't bring into play its health-care effect.Is thereby what the material of the promotion bifidus bacillus propagation that a lot of scientists are contained in exploring breast milk? so the notion of bifidus factor has just been arranged.Enter after the seventies, the scientific worker confirms that the oligomeric galactose that contains in the breast milk is only the material that promotes bifidus bacillus propagation, has so just promoted research and development and production to bifidus factor greatly.
The bifidus factor oligose of producing both at home and abroad mainly contains at present: oligofructose, dextrinosan, soybean oligosaccharide, xylo-oligosaccharide, oligomeric galactose etc., wherein except that soybean oligosaccharide, great majority adopt Production by Enzymes.And these oligose are mixtures of several oligose, and not good to the cultivation effect of bifidus bacillus.Simultaneously, because technology, these oligose can only come into the market with liquid form mostly, and it is not too convenient to transport.
The high purity stachyose that the present invention produces is a kind of white solid powder, and stachyose content is up to 85-95%.And the soybean oligosaccharide syrup that Japan is produced consists of: water 24%, stachyose 18%, raffinose 6%, sucrose 34% and other carbohydrates 18%.In addition, do not have the report that enzyme process or chemical method are produced stachyose yet, it serves to show that the present invention produces the height of stachyose purity.
Find all that through test of many times research naturally occurring stachyose has very strong bifidus bacillus cultivation effect, this is relevant with α-galactosyl that it has, and its physiological property is as follows:
1. be difficult to digestibility: owing to be the α type, intravital beta-galactosidase enzymes can not decompose, and the sucrose position has connected 2 semi-lactosis in the molecule, and sucrase also is difficult to effect, and there is not alpha-galactosidase in human body alimentary canal, so the awkward digestibility sugar of sugar.Secondly it has certain stability to hydrochloric acid in gastric juice, so can arrive at enteron aisle smoothly, has reconfirmed its indigestibility.
2. for the cultivation effect of bifidus bacillus: make bifidus bacillus reach the purpose of advantage flora, only make significantly propagation and sugared source that deleterious spoilage organism can not or seldom utilize is only optimal of bifidus bacillus in the intestines.Stachyose improves the desirable sugared source of intestinal environment just.Figure below is listed several oligose in the in vitro tests to the proliferative of intestinal bacterium.
Several oligose in the in vitro tests are to the proliferative of intestinal bacterium
Annotate: n is the experimental strain number.The evaluation of bacterium fertility :-: 0.099; ±: 0.100-0.199; +: 0.200-0.399; ++: 0.400-0.599; +++:＞0.600
| Intestinal bacterium | ?n | Refining soybean oligosaccharide | Raffinose | Stachyose | Oligofructose |
| Bifidumbacterium bifidum bifidus longum bb bifidobacterium breve bifidobacteria infantis bifidobacterium adolescentis | ?6 ?8 ?3 ?2 ?8 | ????- ????+++ ????+++ ????+++ ????++ | ????- ????+++ ????+++ ????+++ ????++ | ????± ????????+++ ????+++ ????+++ ????++ | ????- ????++ ????+ ????++ ????++ |
| Lactobacterium acidophilum lactobacillus salivarius lactobacterium casei bacterium lactis aerogenes | ?2 ?2 ?2 ?1 | ????± ????????++ ????- ????+ | ????± ????????++ ????- ????+ | ????± ????????++ ????- ????- | ????± ????????+ ????- ????+ |
| Other bacterioides of bacteroides fragilis bacteroides distasonis bacteroides vulgatus bacteroides thetaiotaomicron | ?2 ?5 ?3 ?2 ?4 | ????+ ????+ ????± ????????- ????- | ????+ ????± ????????± ????????± ????????- | ????+ ????+ ????+ ????± ????????± | ????+ ????± ????????+ ????- ????- |
| Other clostridiums of clostridieum welchii class clostridium septicum clostridium difficile clostridium butylicum | ?6 ?4 ?2 ?2 ?7 | ????- ????- ????- ????+ ????- | ????- ????- ????- ????+ ????- | ????- ????- ????- ????+ ????± | ????- ????- ????- ????± ????????± |
| Eubacterium | ?7 | ????± | ????± | ????± | ????- |
| Propionibacterium | ?2 | ????- | ????± | ????- | ????- |
| Fusobacterium | ?3 | ????- | ????± | ????- | ????± |
| Peptostreptococcus | ?6 | ????- | ????- | ????- | ????± |
| Suis | ?2 | ????± | ????± | ????+ | ????± |
| Enterococcus faecalis | ?1 | ????- | ????- | ????- | ????- |
| Wei Rong Shi coccus | ?2 | ????- | ????- | ????- | ????- |
| Intestinal bacteria | ?6 | ????- | ????- | ????- | ????- |
| Enterobacteria | ?1 | ????- | ????- | ????- | ????- |
The objective of the invention is to excavate native resource; protect rare species; make full use of this characteristic plant of kobold; the stachyose purity height that a kind of high efficiency extraction that provides and process for purification are produced; can breed bifidus bacillus in the intestines significantly; impel intestinal acidityization, and can suppress the formation and the cholesterol rising of spoilage product, have the effect that improves calcium, magnesium elements absorption.
------kobold, its place name claims the ground ring again, carries Lou, Jerusalem artichoke, grassy marsh orchid, non-irrigated lotus root, and is voluminous in Shaanxi, provinces such as Henan for achieving the above object, to the present invention relates to a kind of plant of distinct Chinese characteristics, a kind of rare species.This crop is suitable for temperate zone growth, and soil property for well, is not liked fertilely with sandy soil, and the ground that water is arranged is born in happiness, does not need too many field management, is suitable for the mountain area and plants.
Through test of many times, we think that master operation has: effective constituent leaches, removes various impurity, is condensed into powder etc.Best technical process is shown in Fig. 1 (Figure of description), and test-results confirms: water mixes with ethanol when the back is continuous leaches, and can make extraction rate reached more than 85%, and therefore selecting water and ethanol is extracting solution, and both blending ratios are:
Water: 70-80%; Ethanol: 0-30%
Precipitation agent of the present invention adopts the food grade industrial chemicals, and its prescription is as follows:
Bentonite: 40-100%; Diatomite: 0-50%
The present invention's enzymolysis process temperature of lowering the temperature is 30-80 ℃, and used enzyme is a polygalacturonase.It is 1000 that membrane filtration adopts the molecular weight cut-off of film, can make solution 95% or more through entering next procedure after circulation.
Cationic, anionic exchange resin of the present invention mainly rises removes the positive and negative ionization, specific conductivity is significantly descended, and coloring matter is removed, and final each parameter of syrup is:
PH：6-8
Specific conductivity: 6-20 μ s
Stachyose purity: 85-95%
Sugar amount: 20-30 °
Proportion: 1.05-1.20 is again through concentrating, promptly can be made into the white solid powder after the spraying drying.
Characteristics of the present invention are to use traditional continuous leaching-out technique, and in conjunction with modern separation and purification technology, as: membrane separation technique, ion exchange technique make that stachyose content is higher than the level of similar products at home and abroad 30-50% far away up to 85-95% in the product, occupy leading in the world.The membrane separation technique that adopts belongs to a kind of clearer production technology, reduced because of adding the waste residue that other precipitation agents produce, improved production efficiency to greatest extent, reduced disposal of pollutants, the demand that meets contemporary people, be the technology that a kind of economic benefit and environmental benefit are laid equal stress on, meet the win-win strategy of Sustainable development.Stachyose is as a novel bifidus factor, and its activity is very high.The data of delivering according to Japanese saccharic association: in the saccharic with bifidus bacillus propagation, the general oligose of effective intake of average every day (removing xylo-oligosaccharide) needs 8-15g, and stachyose only needs 3g, it serves to show the height of stachyose activity.
This stachyose is the outstanding person in the oligose, and it is remarkable to improve the intestinal environment effect.As us diarrhoea and constipation person are taken observation, the result: these goods experimenter group mean sufferer releasing time is 1.7 days, and patient's mental status recovers after 10 days, and patient's resistibility strengthens after 20 days, returns to one's perfect health.
For estimating the security of this stachyose, carried out mouse LD50 test, experimental results show that stachyose belongs to nontoxic level material, referring to figure below.
The safety testing of stachyose
Annotate: Horn ' s method LD50＞21500 μ l/kg B.W
| Dosage (μ l/kg) | Number of mice (only) male and female | Dead mouse is counted male and female |
| ????2150 ????4640 ????10000 ????21500 | ????5????5 ????5????5 ????5????5 ????5????5 | ????0????0 ????0????0 ????0????0 ????0????0 |
Regulate the ability of intestinal microflora for estimating this stachyose, we have carried out regulating the test of intestinal microflora.Stachyose human body recommended amounts is everyone 3g every day, it is everyone 3g/ every day day/60kg body weight, be equivalent to 0.05g/ day/kg, the mouse dose,equivalent is ten times of human body recommended amounts, it is 0.5g/kg body weight every day (middle dosage), upper and lowerly respectively establish a dosage group: 1.5g/kg body weight (high dosage) and 0.25g/kg body weight (low dosage), experimental data is checked with t, adopts ststa software to add up.Stachyose is to the figure below that the results are shown in of mouse intestinal flora regulating effect.
Stachyose is to the result of mouse intestinal flora regulating effect
| The normal control group | Low dose group | Middle dosage group | High dose group | |||||
| Before gavaging | After gavaging | Before gavaging | After gavaging | Before gavaging | After gavaging | Before gavaging | After gavaging | |
| Enterobacteria | 6.49 ±1.15 | ?6.77 ?±0.58 | ?6.39 ?±2.25 | ?6.36 ?±0.42 | ?6.59 ?±0.61 | ?6.75 ?±0.58 | 6.48 ±0.52 | ?6.22 ?±0.70 |
| Faecalis | 8.26 ±0.48 | ?8.22 ?±0.68 | ?8.20 ?±0.84 | ?7.80 ?±0.41 | ?8.10 ?±0.79 | ?7.68 ?±0.20 ?※ | 8.32 ±0.64 | ?7.48 ?±0.37 ?**※※ |
| Bifidus bacillus | 8.51 ±0.26 | ?8.63 ?±0.35 | ?8.28 ?±0.57 | ?8.95 ?±0.55 ?* | ?8.35 ?±0.48 | ?9.20 ?±0.42 ?**※※ | 8.72 ±0.41 | ?9.40 ?±0.41 ?**※※ |
| Bacterium lacticum | 8.41 ±0.86 | ?8.39 ?±0.71 | ?8.27 ?±0.81 | ?8.85 ?±0.42 | ?8.23 ?±0.90 | ?9.29 ?±0.34 ?**※※ | 8.24 ±0.99 | ?9.24 ?±0.36 ?**※※ |
* with before gavaging compare p≤0.05 * * and gavage preceding relatively p≤0.01
Compare after ※ and normal control group gavage and compare p≤0.01 in sum after p≤0.05 ※ ※ and normal control group gavage, utilize continuous leaching-out technique, the high purity stachyose that membrane separation technique and ion exchange technique are produced with cleaning technique has tangible physiological characteristic, through domestic and international online retrieval, only find the soybean oligosaccharide that from soybean, extracts, the soybean oligosaccharide that contains stachyose and raffinose 30% as japanese food industrial research centre report, the domestic report that extraction stachyose from glutinous rehmannia is also arranged, but its purity is low and production method falls behind, and can't compare with the present invention.Therefore, the advance that possesses skills of the present invention and the practicality of application.
Claims (4)
1. distinct Chinese characteristics plant is characterized in that: it is local to encircle by namely, carries Lou, Jerusalem artichoke, grassy marsh orchid, non-irrigated lotus root.
2. according to right 1 described plant, it is characterized in that: technological process is: raw material → the leach continuously → boil → enzymolysis → membrane sepn of lowering the temperature → ion-exchange → concentrate → spraying drying.
3. according to right 2: it is characterized in that: (1) vat liquor ratio when leaching continuously is as follows:
Water: 70-80%
Ethanol: 0-30%
(2) boiling institute adds the precipitation agent composition and is:
Bentonite: 40-100%
Diatomite: 0-50%
When (3) lowering the temperature enzymolysis, temperature is 30-80 ℃, and enzyme is a polygalacturonase.
(4) adopt membrane separation technique.
(5) adopt ion exchange technique to decolour, desalt.
4. according to syrup parameter after the right 2 described ion-exchanges be:
PH：6-8
Specific conductivity: 6-20 μ s
Stachyose purity: 85-95%
Sugar amount: 20-30 °
Proportion: 1.05-1.20
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| Application Number | Priority Date | Filing Date | Title |
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| CN 01124066 CN1199978C (en) | 2001-08-13 | 2001-08-13 | Process for extracting high purity stachyose from stachys sieboldii |
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| CN 01124066 CN1199978C (en) | 2001-08-13 | 2001-08-13 | Process for extracting high purity stachyose from stachys sieboldii |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100457765C (en) * | 2007-01-16 | 2009-02-04 | 广东太阳神集团有限公司 | Method for producing stabhyose, and method for producing stabhyose and catalpol by using rehmannia root |
| CN102020516A (en) * | 2009-09-14 | 2011-04-20 | 高鹏 | Stachyose containing bio-organic fertilizer and production method thereof |
| CN102174117A (en) * | 2011-01-14 | 2011-09-07 | 南京农业大学 | Method for preparing Stachys floridana schuttl.Exbent. polysaccharide with antitumor activity |
| CN103265583A (en) * | 2013-03-05 | 2013-08-28 | 中国食品发酵工业研究院 | Method for preparing stachyose crystal |
| CN105456407A (en) * | 2015-12-16 | 2016-04-06 | 西安膳方医药科技有限公司 | Novel functional plant composition and application thereof |
-
2001
- 2001-08-13 CN CN 01124066 patent/CN1199978C/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100457765C (en) * | 2007-01-16 | 2009-02-04 | 广东太阳神集团有限公司 | Method for producing stabhyose, and method for producing stabhyose and catalpol by using rehmannia root |
| CN102020516A (en) * | 2009-09-14 | 2011-04-20 | 高鹏 | Stachyose containing bio-organic fertilizer and production method thereof |
| CN102174117A (en) * | 2011-01-14 | 2011-09-07 | 南京农业大学 | Method for preparing Stachys floridana schuttl.Exbent. polysaccharide with antitumor activity |
| CN102174117B (en) * | 2011-01-14 | 2012-11-14 | 南京农业大学 | Method for preparing Stachys floridana schuttl.Exbent. polysaccharide with antitumor activity |
| CN103265583A (en) * | 2013-03-05 | 2013-08-28 | 中国食品发酵工业研究院 | Method for preparing stachyose crystal |
| CN103265583B (en) * | 2013-03-05 | 2016-01-13 | 中国食品发酵工业研究院 | A kind of preparation method of stachyose crystal |
| CN105456407A (en) * | 2015-12-16 | 2016-04-06 | 西安膳方医药科技有限公司 | Novel functional plant composition and application thereof |
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