CN204752588U - Microwave -assisted draws lentinan's equipment - Google Patents
Microwave -assisted draws lentinan's equipment Download PDFInfo
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- CN204752588U CN204752588U CN201520259275.7U CN201520259275U CN204752588U CN 204752588 U CN204752588 U CN 204752588U CN 201520259275 U CN201520259275 U CN 201520259275U CN 204752588 U CN204752588 U CN 204752588U
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Abstract
The utility model relates to a microwave -assisted draws lentinan's equipment, equipment is including the extraction element, decoloration device, purification device, the enrichment facility that set up in order, the extraction element includes the abstraction reaction ware, abstraction reaction ware upper end is equipped with enzyme preparation inlet port, install microwave generator on the abstraction reaction ware, the intussuseption of decoloration device is filled with macroporous resin, the macroporous resin particle diameter is 30-50 meshes, extraction element, decoloration device, purification device, enrichment facility are in order through the pipeline intercommunication, be equipped with filter equipment between extraction element and the decoloration device. The utility model discloses extracting the enzyme and combining with the microwave -assisted extract phase, not only easily industrial production has advantages such as the flow is short, simple process economy, and the lentinan who obtains through this equipment preparation in addition has characteristics such as extraction percentage height, purity height, has guaranteed simultaneously that lentinan's biological activity is not destroyed.
Description
Technical field
The utility model relates to a kind of Polyose extraction equipment, especially relates to a kind of equipment of microwave radiation exaraction lentinan.
Background technology
Mushroom is the basidiomycetes of Pleurotaceae, delicious flavour, is medicine-food two-purpose fungi.Particularly lentinan (Lentinan) wherein has significant anti-tumor activity, and can alleviate the toxic side effect of radiation and chemotherapy.Japanese scholars thousand in 1969 is former takes the lead in confirming the anti-tumor activity of mushroom hot water extraction thing, plumage field, assistant wood further research confirm that its effective constituent is lentinan, CoroChihara lixiviate from mushroom fruiting body goes out 6 kinds of lentinans, and prove that wherein one has obvious antitumor action, names as Lentinan.Within 1980, the second-class confirmation lentinan of trust is Th cell activator, and it makes body's immunity be restored and improves and suppress, engulf tumour cell, can be used for auxiliary cancer therapy.Lentinan is also to serum GPT(transaminase) there is obvious restraining effect, Lu Yongzhi etc. (1985) have done further checking from dynamic characteristic aspect.Simple literary compositions etc. (1986) also confirm that the extract containing lentinan has the effect of platelet aggregation-against.Some researchs simultaneously prove, lentinan can also improve SOD activity and reduce lipofuscin content and antifatigue effect.Because lentinan has good nutrition, medicinal and health value, recent domestic conducts extensive research utilization to it.
Lentinan finished product is white powdery solids, to light and thermally stable.In water, maxima solubility is 3g/L; Can be dissolved in 0.5mol/L sodium hydroxide, solubleness can reach 50g/L ~ 100g/L; Be insoluble in methyl alcohol, ethanol, acetone and other organic solvent.Lentinan has water absorbability, is 92.5% in relative humidity, and temperature is place 15 days in the environment of 25 DEG C, and water regain can reach 40%.At present, the main extracting method of lentinan has water extraction and alcohol precipitation method, extraction etc., but there is the shortcomings such as extraction process complexity, solvent usage quantity is large, the time is long, and easily causes polysaccharide to degrade, and biological activity reduces, and extraction yield is lower.
In addition, the polysaccharide soln extracted from mushroom, plant, general containing darker color, the existence of pigment affects purity and the theoretical investigation of polysaccharide, therefore will remove as far as possible, and conventional decoloring method has H
2o
2method, activated carbon method and ion-exchange-resin process etc., polysaccharide decolouring is a critical process of polysaccharide purification, and the polysaccharide decolouring darker for color does not still have desirable method.Existing method mainly adopts charcoal absorption to decolour, and the method decolorizing effect is general, and gac is serious to the absorption of polysaccharide, H
2o
2also for polysaccharide decolouring, although decolorizing effect is pretty good, there is the problem of polysaccharide degraded, this probably affects the biological activity of polysaccharide, therefore uses its decolouring to consider carefully.Ion exchange resin uses DEAE cellulose column usually, because DEAE Mierocrystalline cellulose is expensive, generally can only extract high purity polysaccharide and use in theoretical investigation, can not apply in suitability for industrialized production.
Extraction is the critical process of separation of polysaccharides purifying, and be the most basic work of research polysaccharide, its leaching process, technique, purity of polysaccharide directly affect the biological activity of polysaccharide, therefore, study efficient, economic lentinan extracting and developing method and have realistic meaning.
Utility model content
In order to overcome the deficiency that prior art exists, the utility model provides a kind of equipment of microwave radiation exaraction lentinan.
A kind of equipment of microwave radiation exaraction lentinan, described equipment comprises the extraction element, decoloration device, purification devices, the concentrating unit that arrange in turn, described extraction element comprises extraction reactor, described extraction reactor upper end is provided with zymin and adds entrance, and described extraction reactor is provided with microwave generator; Be filled with macroporous resin in described decoloration device, described macroporous resin particle diameter is 30 ~ 50 orders; Described extraction element, decoloration device, purification devices, concentrating unit sequentially pass through pipeline communication; Filtration unit is provided with between described extraction element and decoloration device.
As preferably, described equipment also comprises washing unit, drying installation and proofing unit, before described washing unit is arranged on extraction element, after drying installation is arranged on concentrating unit, after described proofing unit is arranged on drying installation, described proofing unit comprises spectrophotometer and high performance liquid chromatograph.
As preferably, described purification devices is pottery membrane microfiltration device, and described concentrating unit comprises rotary evaporating device, and described drying installation is freeze drier.
Concerning the extracting and developing of polysaccharide, note the problem of two aspects, one is the yield of polysaccharide, and two is the purity of polysaccharide, in the yield ensureing that polysaccharide biological activity can not be destroyed in leaching process, the purity of gained polysaccharide improves polysaccharide under not affecting the prerequisite of correlative study as far as possible.Extraction is the critical process of separation of polysaccharides purifying, research polysaccharide most basic work, its leaching process, technique, purity of polysaccharide directly affect polysaccharide biological activity, therefore, study efficient, economic lentinan extracting and developing method there is realistic meaning.
Advantage of the present utility model is:
Zymin pre-treatment: add corresponding enzyme to decompose mushroom cell walls in the process of lixiviate lentinan, promote polysaccharide release, increase the extraction yield of lentinan, corresponding protein enzyme also contributes to reducing protein content in extracting solution, reduce purification time and operation, improve purity of polysaccharide;
Microwave radiation exaraction: the heating effect and the biological wall breaking effect that utilize microwave, greatly can shorten experiment and production time, reduce energy consumption, minimizing solvent load and the generation of refuse, yield and extract purity can be improved simultaneously, its superiority is not only to reduce experimental implementation expense and production cost, the more important thing is that this technology meets the requirement of " green " environmental protection more;
Grading purification: ultra-filtration and separation purified polysaccharide is also be separated with the difference of shape according to polysaccharide molecular weight, the ultra-filtration membrane of PSPP can optionally through the polysaccharide that molecular weight or volume vary in size, and polysaccharide is carried out separation and purification, there is certain inspissated to polysaccharide extraction liquid, reduce concentration time and energy consumption.First carry out micro-filtration and remove supramolecular and particulate matter, then select and carry out ultrafiltration through membrane molecule amount, obtain the purifying lentinan in different fractionated molecule amount interval.Ultra-filtration and separation equipment is simple, easy to operate, and treatment capacity is large, relative to gel filtration chromatography, is applicable to suitability for industrialized production polysaccharide.
Enzyme extraction combines with microwave radiation exaraction by the utility model, is not only easy to suitability for industrialized production, has the advantages such as flow process is short, technique simple economy, and is had by the lentinan that this technique prepares that extraction yield is high, purity high.Ensure that the biological activity of lentinan is not destroyed simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model equipment;
Fig. 2 is the utility model process route chart.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail, but protection domain of the present utility model is not limited to this.
With reference to Fig. 1, a kind of equipment of microwave radiation exaraction lentinan, described equipment comprises the extraction element 1, decoloration device 2, purification devices 3, the concentrating unit 4 that arrange in turn, described extraction element 1 comprises extraction reactor, described extraction reactor upper end is provided with zymin and adds entrance 5, described extraction reactor is provided with microwave generator 6; Be filled with macroporous resin in described decoloration device 2, described macroporous resin particle diameter is 30 ~ 50 orders; Described extraction element 1, decoloration device 2, purification devices 3, concentrating unit 4 sequentially pass through pipeline communication; Filtration unit 7 is provided with between described extraction element 1 and decoloration device 2.
Described equipment also comprises washing unit, drying installation and proofing unit, before described washing unit is arranged on extraction element, after drying installation is arranged on concentrating unit, after described proofing unit is arranged on drying installation, described proofing unit comprises spectrophotometer and high performance liquid chromatograph.
Described purification devices is pottery membrane microfiltration device, and described concentrating unit comprises rotary evaporating device (Rotary Evaporators), and described drying installation is freeze drier.
With reference to Fig. 2, aforesaid device, for extracting the technique of lentinan, comprises the steps:
(1) thing pre-treatment to be extracted: mushroom is cleaned and dries, be ground into the meal that particle diameter is 0.25mm;
(2) zymin pre-treatment: thing to be extracted is placed in extraction reactor, adds certain volume water and neutral protease, adjust ph in extraction reactor, to mushroom extract enzymolysis certain hour; Wherein, feed liquid mass ratio is 1:22, neutral protein enzyme dosage 1.0%, and hydrolysis temperature is 50 DEG C, and extraction time is 60min, pH5.0;
(3) microwave extraction: open microwave device, adjustment microwave power is at 600W, and microwave radiation extraction 5min, filters to obtain lentinan extracting solution;
(4) decolorization: adopt particle diameter to be that 40 object DA201-C macroporous resins carry out decolouring purifying to lentinan extracting solution, utilize DA201-C macroporous resin in pH value 4 ~ 6, temperature 40 DEG C, under the processing condition of flow velocity 3BV/h, carries out the desolventing technology of lentinan extracting solution;
(5) grading purification: the lentinan extracting solution after decolouring is put into pottery membrane microfiltration device, carries out grading purification; First carry out micro-filtration and remove supramolecular and particulate matter, then select and carry out ultrafiltration through membrane molecule amount, obtain the purifying lentinan in different fractionated molecule amount interval, and certain inspissated is played to extracting solution;
(6) concentrated, alcohol precipitation process: utilize Rotary Evaporators to be carried out being concentrated to certain proportion by lentinan extracting solution, 95% ethanol adding 4 times of volumes in concentrated solution makes lentinan concentrated solution be settled out lentinan;
(7) drying process: adopt freeze-drying method to make lentinan dry;
(8) detection and structural analysis: carry out polysaccharide content detection by spectrophotometer and high performance liquid chromatograph, and analyzed by the monose composition of column front derivation to lentinan.The content obtaining lentinan product is 91%, and it is 9.12% that lentinan extracts yield.
Claims (3)
1. the equipment of a microwave radiation exaraction lentinan, it is characterized in that: described equipment comprises the extraction element, decoloration device, purification devices, the concentrating unit that arrange in turn, described extraction element comprises extraction reactor, described extraction reactor upper end is provided with zymin and adds entrance, and described extraction reactor is provided with microwave generator; Be filled with macroporous resin in described decoloration device, described macroporous resin particle diameter is 30 ~ 50 orders; Described extraction element, decoloration device, purification devices, concentrating unit sequentially pass through pipeline communication; Filtration unit is provided with between described extraction element and decoloration device.
2. the equipment of microwave radiation exaraction lentinan according to claim 1, it is characterized in that: described equipment also comprises washing unit, drying installation and proofing unit, before described washing unit is arranged on extraction element, after drying installation is arranged on concentrating unit, after described proofing unit is arranged on drying installation, described proofing unit comprises spectrophotometer and high performance liquid chromatograph.
3. the equipment of microwave radiation exaraction lentinan according to claim 1 and 2, is characterized in that: described purification devices is pottery membrane microfiltration device, and described concentrating unit comprises rotary evaporating device, and described drying installation is freeze drier.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520259275.7U CN204752588U (en) | 2015-04-27 | 2015-04-27 | Microwave -assisted draws lentinan's equipment |
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| CN201520259275.7U CN204752588U (en) | 2015-04-27 | 2015-04-27 | Microwave -assisted draws lentinan's equipment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107080025A (en) * | 2017-06-09 | 2017-08-22 | 留坝县中药材发展办公室 | A kind of Xianggu mushroom tea and preparation method thereof |
| CN110862464A (en) * | 2019-11-04 | 2020-03-06 | 东台汉方缘生物科技有限公司 | Extraction and production process of ginseng polysaccharide |
-
2015
- 2015-04-27 CN CN201520259275.7U patent/CN204752588U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107080025A (en) * | 2017-06-09 | 2017-08-22 | 留坝县中药材发展办公室 | A kind of Xianggu mushroom tea and preparation method thereof |
| CN110862464A (en) * | 2019-11-04 | 2020-03-06 | 东台汉方缘生物科技有限公司 | Extraction and production process of ginseng polysaccharide |
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