CN107594472A - A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method - Google Patents
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method Download PDFInfo
- Publication number
- CN107594472A CN107594472A CN201711032804.XA CN201711032804A CN107594472A CN 107594472 A CN107594472 A CN 107594472A CN 201711032804 A CN201711032804 A CN 201711032804A CN 107594472 A CN107594472 A CN 107594472A
- Authority
- CN
- China
- Prior art keywords
- betacyanins
- extraction
- suaeda salsa
- collect
- extraction method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Extraction Or Liquid Replacement (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to Betacyanins extractive technique field, more particularly to a kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, including pretreatment, ultrasonic wave added enzymolysis processing, supercritical extract, subcritical abstraction, purifying, drying steps.Using extracting method in the present invention, the recovery rate and purity of Suaeda salsa Betacyanins product are greatly improved, the yield of biogenic salt is high, and the biogenic salt decolouring removing heavy-metal time is short.
Description
Technical field
The present invention relates to Betacyanins extractive technique field, more particularly to a kind of Suaeda salsa biogenic salt and Betacyanins it is comprehensive
Close extracting method.
Background technology
Betacyanins, it is a kind of natural plant pigment being widely present in plant, by the Betacyanins and beet of red
Flavine forms, and main component is beet red glycosides in Betacyanins, accounts for the 75~95% of haematochrome, and remaining still has different betanin, preceding
Betanin etc..Betacyanins, its anticancer, antiviral and oxidation resistant bioactivity make Betacyanins be added in health products and food
Agent field receives significant attention.
Suaeda salsa is a kind of typical euhalophyte, widely distributed on the salt licks such as Coastal beach, be it is a kind of very
There are the natural resources of development potentiality, there is very big economic benefit, ecological benefits and social benefit.Research has confirmed salt at present
Containing abundant Betacyanins during ground alkali is fluffy, prior art has the report that Betacyanins or biogenic salt are extracted using Suaeda salsa as raw material
Road, but be all the extracting method individually extracted a certain composition and established, and the product purity of gained is low, and yield is low, sweet tea
The technique or method that dish red pigment and biogenic salt extract simultaneously have no report, therefore in view of the above-mentioned problems, are necessary to establish a kind of life
Thing salt and Betacyanins combined extraction method.
The content of the invention
The technical problems to be solved by the invention are：In view of the deficienciess of the prior art, product can be improved by providing one kind
The Suaeda salsa biogenic salt and Betacyanins combined extraction method of purity and recovery rate.
In order to solve the above technical problems, the technical scheme is that：
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, the extracting method comprise the following steps：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:5-1:10
Ratio adds Extraction solvent, homogenate extraction, filtering, collects supernatant and sediment respectively；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, add the compound of weight of precipitate 1-3%
Enzyme, mix, adjust pH, enzyme digestion reaction is carried out after supersound process, enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (3) to collect, according to solid-liquid ratio 1:5-1:10 ratio adds folder
Band agent, is extracted, and extraction temperature is 35-55 DEG C, extracting pressure 3.2-4.8Mpa, CO2Flow be 8-12L/h, extraction
Time is 30-60min, stops extraction, filtering, collects extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:5-1:8 ratio adds pH3-5
Deionized water extracted, extraction temperature be 30-60 DEG C, extracting pressure 2.3-3.0Mpa, CO2Flow be 8-10L/h,
Extraction time is 30-60min, stops extraction, filtering, collects filtrate；
(5) drying is purified：The extract for taking step (3) to collect, will be washed through excessive DEG C of hole resin adsorption again after ultrafiltration
SephadexG-25 dextran chromatography posts in the de- Betacyanins crude product solution collected, then after elution, collection is washed
De- component, by vacuum drying, obtains Betacyanins product；The supernatant that step (1) is collected and the filtrate of step (4) are closed
And obtain biogenic salt production finally by freeze-drying by filtering, the filtrate of collection again by decolourizing, after removing heavy-metal processing
Product.
As an improvement technical scheme, the extraction time of homogenate extraction is 1-5min in step (1), and Extracting temperature is
25-30 DEG C, extraction voltage is 180-210v.
As an improvement technical scheme, in step (1) Extraction solvent be volumetric concentration be 40-80% ethanol with
0.1-0.5M citric acid mixes.
As an improvement technical scheme, the complex enzyme in step (2) is by pectase, cellulase, neutral proteinase
With a- glucuroides according to 1-5:2-7:1-6:1-3 ratio mixes.
As an improvement technical scheme, the reaction conditions of step (2) are：PH3-6.5,25-37 DEG C of temperature, enzyme
The solution time is 1.5-3h.
As an improvement technical scheme, the ultrasonic power being ultrasonically treated in step (2) is 220-460W, ultrasonic time
For 5-15min.
As an improvement technical scheme, the entrainer of step (4) is by acetic acid, Tween-80 and ethanol according to 0.5-2:
0.1-0.5:1-3 ratio mixes, and the mass concentration of the Tween-80 is 5%-10%, and the volumetric concentration of ethanol is
40%-65%.
As an improvement technical scheme, the decolorization flocculation agent added when decolouring in step (5), removing heavy-metal processing
By chitosan, sodium alginate and recessed soil according to 0.1-0.5:0.1-0.3:0.1-0.5 ratio mixes.
As an improvement technical scheme, the macroreticular resin in step (5) is X-5 macroreticular resins.
As an improvement technical scheme, the eluent in step (5) is by tartaric acid and water according to 1:2-5 ratio is mixed
Conjunction forms, and the flow velocity of the eluent is 1-2ml/min.
After employing above-mentioned technical proposal, the beneficial effects of the invention are as follows：
Compared with prior art, the present invention mainly has advantages below：
(1) present invention uses flash extraction, ultrasonic wave added enzymolysis, supercritical extract and subcritical abstraction GC-MS, with
Suaeda salsa is the extraction that raw material carries out Betacyanins and biogenic salt, can make biogenic salt component in a short time by flash pre- carry
Fully discharge, then ultrasonic wave added enzymolysis is carried out as raw material using the flash pre- sediment that obtains carried, by pectase, cellulose
The complex enzyme of enzyme, neutral proteinase and a- glucuroides composition can carry out fully degraded to cell wall structure, further have
Help Betacyanins to discharge from cell interior, while also reduce protein component and polysaccharide component content, then from acetic acid,
Tween-80 and ethanol carry out supercritical extract as entrainer, on the one hand can reduce tension force caused by Extraction solvent surface, promote
Enter Betacyanins component dissolution, on the other hand can prevent Betacyanins from degrading；The sediment that supercritical extract is obtained again is carried out
Subcritical abstraction, further increase the yield of biogenic salt.
(2) present invention isolates and purifies method for combined use using macroporous resin adsorption and sephadex chromatography, by heavy metal and
The other components such as organic acid are effectively separated, and greatly improve the purity of Betacyanins product；The present invention is from chitosan, marine alga
Sour sodium and recessed soil are handled by decolouring, removing heavy-metal to biogenic salt crude product as decolorization flocculation agent, then after filtering, carried significantly
The high purity and decolouring, removing heavy-metal processing time is short of biological product salt.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Example 1
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, comprise the following steps：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:5 ratio
Adding Extraction solvent, (ethanol and 0.1M citric acid that volumetric concentration is 40% are according to 1:0.5 ratio mixes), it is flash
Extract (extraction time 1.5min, Extracting temperature are 25 DEG C, and extraction voltage is 180v), filtering, collect supernatant respectively and sink
Starch；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, add weight of precipitate 1-3% complex enzyme
(by pectase, cellulase, neutral proteinase and a- glucuroides according to 1:2:1:1 ratio mixes), mix, adjust
PH, after supersound process (ultrasonic power 220W, ultrasonic time 5min) carry out enzyme digestion reaction (enzymatic hydrolysis condition pH3.5,
25 DEG C of temperature, enzymolysis time 1.5h), enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (3) to collect, according to solid-liquid ratio 1:5 ratio adds extractant
(acetic acid, Tween-80 and ethanol are according to 0.5:0.1:1 ratio mixes, and the mass concentration of Tween-80 is 5%, ethanol
Volumetric concentration is 40%), to be extracted, and extraction temperature is 35 DEG C, extracting pressure 3.2Mpa, CO2Flow be 8L/h, extraction
Time is 30min, stops extraction, filtering, collects extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:5 ratio add pH3 go from
Sub- water is extracted, and extraction temperature is 30 DEG C, extracting pressure 2.3Mpa, extraction time 30min, stops extraction, is filtered, and is received
Collect filtrate；
(5) drying is purified：The extract for taking step (3) to collect, passes through X-5 macroporous resin adsorptions again after ultrafiltration, will
SephadexG-25 dextran chromatography posts in the Betacyanins crude product solution that deionized water elution is collected, then by elution
(tartaric acid and water are according to 1 afterwards:2 ratio mixes, and the flow velocity of the eluent is 1ml/min), collect elution fraction, warp
Vacuum drying is crossed, obtained Betacyanins product purity is 99.42%, the Betacyanins of Unit Weight stem and leaf of S. salsa (L.) Pall extraction
Product can reach 7.46mg/g；
The supernatant that step (1) is collected and the filtrate of step (4) merge, add decolorization flocculation agent (decolorization flocculation agent by
Chitosan, sodium alginate and recessed soil are according to 0.1:0.1:0.1 ratio mixes) by decolourizing, after removing heavy-metal processing, then
By filtering, the filtrate of collection is finally by freeze-drying, and the purity for obtaining biological product salt is 99.56%, biological product salt
Yield can reach 2.28%, the decolorization time is 18min.
Example 2
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, comprise the following steps：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:8 ratio
Adding Extraction solvent, (ethanol and 0.3M citric acid that volumetric concentration is 45% are according to 1:0.5 ratio mixes), it is flash
Extraction (extraction time 2min, Extracting temperature are 28 DEG C, and extraction voltage is 185v), filtering, collects supernatant and precipitation respectively
Thing；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, add weight of precipitate 1-3% complex enzyme
(by pectase, cellulase, neutral proteinase and a- glucuroides according to 2:3:2:1.5 ratio mixes), mix,
PH is adjusted, (ultrasonic power 260W, ultrasonic time 8min) carries out enzyme digestion reaction (enzymatic hydrolysis condition is after supersound process
PH3.5,28 DEG C of temperature, enzymolysis time 1.8h), enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (3) to collect, according to solid-liquid ratio 1:8 ratio adds extractant
(acetic acid, Tween-80 and ethanol are according to 0.8:0.3:1.5 ratio mixes, and the mass concentration of Tween-80 is 6.8%, second
45%) volumetric concentration of alcohol is, is extracted, extraction temperature is 40 DEG C, extracting pressure 3.5Mpa, CO2Flow be 9L/h,
Extraction time is 35min, stops extraction, filtering, collects extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:6 ratio adds going for pH3.5
Ionized water is extracted, and extraction temperature is 35 DEG C, extracting pressure 2.5Mpa, extraction time 35min, stops extraction, is filtered,
Collect filtrate；
(5) drying is purified：The extract for taking step (3) to collect, passes through X-5 macroporous resin adsorptions again after ultrafiltration, will
SephadexG-25 dextran chromatography posts in the Betacyanins crude product solution that deionized water elution is collected, then by elution
(tartaric acid and water are according to 1 afterwards:3 ratio mixes, and the flow velocity of the eluent is 1.5ml/min), elution fraction is collected,
By vacuum drying, obtained Betacyanins product purity is 99.65%, the beet red of Unit Weight stem and leaf of S. salsa (L.) Pall extraction
Plain product can reach 7.8mg/g；
The supernatant that step (1) is collected and the filtrate of step (4) merge, add decolorization flocculation agent (decolorization flocculation agent by
Chitosan, sodium alginate and recessed soil are according to 0.1-0.5:0.1-0.3:0.1-0.5 ratio mixes) by decolourizing, taking off weight
After metal processing, then by filtering, finally by freeze-drying, the purity for obtaining biological product salt is the filtrate of collection
99.62%, the yield of biological product salt can reach 2.36%, and the decolorization time is 15min.
Example 3
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, comprise the following steps：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:10 ratio
Adding Extraction solvent, (ethanol and 0.45M citric acid that volumetric concentration is 75% are according to 1：0.5 ratio mixes), it is flash
Extract (extraction time 4min, Extracting temperature are 28.5 DEG C, and extraction voltage is 202v), filtering, collect supernatant respectively and sink
Starch；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, add weight of precipitate 1-3% complex enzyme
(by pectase, cellulase, neutral proteinase and a- glucuroides according to 4.5:6:5:2.7 ratio mixes), mix
It is even, pH is adjusted, (ultrasonic power 420W, ultrasonic time 12min) carries out enzyme digestion reaction (enzymatic hydrolysis condition is after supersound process
PH6.2,36 DEG C of temperature, enzymolysis time 2.5h), enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (3) to collect, according to solid-liquid ratio 1:8 ratio adds extractant
(acetic acid, Tween-80 and ethanol are according to 1.5:0.4:2.8 ratio mixes, and the mass concentration of Tween-80 is 8.8%, second
60%) volumetric concentration of alcohol is, is extracted, extraction temperature is 53 DEG C, extracting pressure 4.5Mpa, CO2Flow be 11L/
H, extraction time 55min, stop extraction, filtering, collect extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:8 ratio adds going for pH4.8
Ionized water is extracted, and extraction temperature is 58 DEG C, extracting pressure 2.8Mpa, extraction time 55min, stops extraction, is filtered,
Collect filtrate；
(5) drying is purified：The extract for taking step (3) to collect, passes through X-5 macroporous resin adsorptions again after ultrafiltration, will
SephadexG-25 dextran chromatography posts in the Betacyanins crude product solution that deionized water elution is collected, then by elution
(tartaric acid and water are according to 1 afterwards:4.5 ratio mixes, and the flow velocity of the eluent is 1.8ml/min), collect elution group
Point, by vacuum drying, obtained Betacyanins product purity is 99.79%, the sweet tea of Unit Weight stem and leaf of S. salsa (L.) Pall extraction
Dish red pigment product can reach 8.26mg/g；
The supernatant that step (1) is collected and the filtrate of step (4) merge, add decolorization flocculation agent (decolorization flocculation agent by
Chitosan, sodium alginate and recessed soil are according to 0.4:0.2:0.3 ratio mixes) by decolourizing, after removing heavy-metal processing, then
By filtering, the filtrate of collection is finally by freeze-drying, and the purity for obtaining biological product salt is 99.78%, biological product salt
Yield can reach 2.54%, the decolorization time is 5min.
Example 4
A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, comprise the following steps：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:5-1:10
Ratio adds Extraction solvent (volumetric concentration is that 80% ethanol and 0.5M citric acid mix), and homogenate extraction is (during extraction
Between be 5min, Extracting temperature is 30 DEG C, and extraction voltage be 210v), filter, respectively collection supernatant and sediment；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, add the complex enzyme of weight of precipitate 3%
(by pectase, cellulase, neutral proteinase and a- glucuroides according to 5:7:6:3 ratio mixes), mix, adjust
PH, (ultrasonic power 460W, ultrasonic time 15min) carries out enzyme digestion reaction (enzymatic hydrolysis condition is after supersound process
PH6.5,37 DEG C of temperature, enzymolysis time 3h), enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (3) to collect, according to solid-liquid ratio 1:10 ratio adds extractant
(acetic acid, Tween-80 and ethanol are according to 2:0.5:3 ratio mixes, and the mass concentration of Tween-80 is 9.8%, ethanol
Volumetric concentration is 65%), to be extracted, and extraction temperature is 55 DEG C, extracting pressure 4.8Mpa, CO2Flow be 12L/h, extraction
It is 60min to take the time, stops extraction, filtering, collects extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:8 ratio add pH5 go from
Sub- water is extracted, and extraction temperature is 60 DEG C, extracting pressure 3.0Mpa, extraction time 60min, stops extraction, is filtered, and is received
Collect filtrate；
(5) drying is purified：The extract for taking step (3) to collect, passes through X-5 macroporous resin adsorptions again after ultrafiltration, will
SephadexG-25 dextran chromatography posts in the Betacyanins crude product solution that deionized water elution is collected, then by elution
(tartaric acid and water are according to 1 afterwards:5 ratio mixes, and the flow velocity of the eluent is 2ml/min), collect elution fraction, warp
Vacuum drying is crossed, obtained Betacyanins product purity is 99.75%, the Betacyanins of Unit Weight stem and leaf of S. salsa (L.) Pall extraction
Product can reach 8.15mg/g；
The supernatant that step (1) is collected and the filtrate of step (4) merge, add decolorization flocculation agent (decolorization flocculation agent by
Chitosan, sodium alginate and recessed soil are according to 0.5:0.3:0.5 ratio mixes) by decolourizing, after removing heavy-metal processing, then
By filtering, the filtrate of collection is finally by freeze-drying, and the purity for obtaining biological product salt is 99.76%, biological product salt
Yield can reach 2.52%, the decolorization time is 8min.
In order to which Betacyanins product can be improved when preferably proving using the extracting method of the present invention to extract anthocyanidin
Purity and recovery rate, the present invention have done 3 comparative examples simultaneously.
Comparative example 1
As different from Example 3, ultrasonic wave added enzymolysis step is lacked, remaining condition is identical, the beet that comparative example 1 is extracted
The purity of red pigment product is 99.75%, and the Betacyanins product of Unit Weight stem and leaf of S. salsa (L.) Pall extraction can reach 7.2mg/g；
The purity 99.74% of biological product salt, the yield 2.24% of biological product salt, the time of decolouring removing heavy-metal processing is 6min.
Comparative example 2
As different from Example 3, subcritical abstraction step is lacked, remaining condition is identical, the beet red that comparative example 2 is extracted
The purity of plain product is 99.77%, and the Betacyanins product of Unit Weight stem and leaf of S. salsa (L.) Pall extraction can reach 8.21mg/g；It is raw
The purity 99.73% of thing product salt, the yield 2.45% of biological product salt, the time of decolouring removing heavy-metal processing is 6min.
Comparative example 3
As different from Example 3, only adsorbed when Betacyanins purify from macroreticular resin, biogenic salt decolourizes de- heavy
Metal processing when decolorization flocculation agent be aluminium polychloride, remaining condition is identical, comparative example 3 extraction Betacyanins product it is pure
Spend for 99.56%, the Betacyanins product of Unit Weight stem and leaf of S. salsa (L.) Pall extraction can reach 8.05mg/g, biological product salt
Purity 99.52%, the yield 2.51% of biological product salt, the time of decolouring removing heavy-metal processing is 30min.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method, it is characterised in that the extracting method includes following
Step：
(1) pretreatment of raw material：By the stem and leaf of S. salsa (L.) Pall of fresh harvesting, clean, dry, according to solid-liquid ratio 1:5-1:10 ratio
Extraction solvent is added, homogenate extraction, filtering, collects supernatant and sediment respectively；
(2) ultrasonic wave added enzymolysis processing：The sediment for taking step (1) to collect, weight of precipitate 1-3% complex enzyme is added, mixed
It is even, pH is adjusted, enzyme digestion reaction is carried out after supersound process, enzymolysis collects enzymolysis product after terminating；
(3) supercritical extract：The enzymolysis product for taking step (2) to collect, according to solid-liquid ratio 1:5-1:10 ratio adds entrainer,
Extracted, extraction temperature is 35-55 DEG C, extracting pressure 3.2-4.8Mpa, CO2Flow be 8-12L/h, extraction time is
30-60min, stop extraction, filtering, collect extract and sediment respectively；
(4) subcritical abstraction：The sediment for taking step (3) to collect, according to solid-liquid ratio 1:5-1:8 ratio adds going for pH3-5
Ionized water is extracted, and extraction temperature is 30-60 DEG C, extracting pressure 2.3-3.0Mpa, extraction time 30-60min, is stopped
Extraction, filtering, collect filtrate；
(5) drying is purified：The extract for taking step (3) to collect, elution is received through excessive DEG C of hole resin adsorption again after ultrafiltration
SephadexG-25 dextran chromatography posts in the Betacyanins crude product solution of collection, then after elution, collect elution group
Point, by vacuum drying, obtain Betacyanins product；The supernatant that step (1) is collected and the filtrate of step (4) merge, warp
Cross after decolouring, removing heavy-metal processing and obtain biological product salt finally by freeze-drying by filtering, the filtrate of collection again.
2. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
The extraction time of homogenate extraction is 1-5min in step (1), and Extracting temperature is 25-30 DEG C, and extraction voltage is 180-210v.
3. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
Extraction solvent is that ethanol that volumetric concentration is 40-80% and 0.1-0.5M citric acid mix in step (1).
4. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
Complex enzyme in step (2) is by pectase, cellulase, neutral proteinase and a- glucuroides according to 1-5:2-7:1-6:1-
3 ratio mixes.
5. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
The reaction conditions of step (2) are：PH3-6.5,25-37 DEG C of temperature, enzymolysis time 1.5-3h.
6. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
The ultrasonic power being ultrasonically treated in step (2) is 220-460W, ultrasonic time 5-15min.
7. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
The entrainer of step (3) is by acetic acid, Tween-80 and ethanol according to 0.5-2:0.1-0.5:1-3 ratio mixes, described to tell
The mass concentration of temperature -80 is 5%-10%, and the volumetric concentration of ethanol is 40%-65%.
8. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
The decolorization flocculation agent that decolouring, removing heavy-metal in step (5) add when handling is by chitosan, sodium alginate and recessed soil according to 0.1-
0.5:0.1-0.3:0.1-0.5 ratio mixes.
9. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, it is characterised in that：
Macroreticular resin in step (5) is X-5 macroreticular resins.
10. a kind of Suaeda salsa biogenic salt according to claim 1 and Betacyanins combined extraction method, its feature exist
In：Eluent in step (5) is by tartaric acid and water according to 1:2-5 ratio mixes, and the flow velocity of the eluent is 1-
2ml/min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711032804.XA CN107594472A (en) | 2017-10-30 | 2017-10-30 | A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711032804.XA CN107594472A (en) | 2017-10-30 | 2017-10-30 | A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107594472A true CN107594472A (en) | 2018-01-19 |
Family
ID=61083677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711032804.XA Pending CN107594472A (en) | 2017-10-30 | 2017-10-30 | A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107594472A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110041727A (en) * | 2019-05-17 | 2019-07-23 | 石河子大学 | A kind of method of ultrasound assisted extraction beet red pigment |
| CN111480828A (en) * | 2020-04-24 | 2020-08-04 | 山东省蚕业研究所 | Method for co-extracting betacyanin and plant salt in suaeda salsa |
| CN111925402A (en) * | 2020-08-03 | 2020-11-13 | 北京师范大学 | Method for separating and refining multiple betacyanins from suaeda salsa |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204217A (en) * | 2007-12-20 | 2008-06-25 | 山东师范大学 | Method for extracting beet red pigment from Suaeda salsa |
| CN102321384A (en) * | 2011-06-21 | 2012-01-18 | 西北大学 | Liquid-phase supercritical method for removing and separating spiciness from capsicum red pigment and device special for liquid-phase critical method |
| CN102551028A (en) * | 2012-03-01 | 2012-07-11 | 山东凯尔海洋生物科技有限公司 | Method for preparing suaeda salsa biogenetic salt |
| CN102653634A (en) * | 2011-03-02 | 2012-09-05 | 盐城工学院 | Ultrasound-assisted method for extracting betacyanin from suaeda salsa |
| CN105876744A (en) * | 2016-04-19 | 2016-08-24 | 王胜 | Vegetable salt and method for extracting vegetable salt from suaeda salsa by adopting three-stage enzymolysis technology |
-
2017
- 2017-10-30 CN CN201711032804.XA patent/CN107594472A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204217A (en) * | 2007-12-20 | 2008-06-25 | 山东师范大学 | Method for extracting beet red pigment from Suaeda salsa |
| CN102653634A (en) * | 2011-03-02 | 2012-09-05 | 盐城工学院 | Ultrasound-assisted method for extracting betacyanin from suaeda salsa |
| CN102321384A (en) * | 2011-06-21 | 2012-01-18 | 西北大学 | Liquid-phase supercritical method for removing and separating spiciness from capsicum red pigment and device special for liquid-phase critical method |
| CN102551028A (en) * | 2012-03-01 | 2012-07-11 | 山东凯尔海洋生物科技有限公司 | Method for preparing suaeda salsa biogenetic salt |
| CN105876744A (en) * | 2016-04-19 | 2016-08-24 | 王胜 | Vegetable salt and method for extracting vegetable salt from suaeda salsa by adopting three-stage enzymolysis technology |
Non-Patent Citations (1)
| Title |
|---|
| 段迪: "盐地碱蓬甜菜红素提取分离及纯化工艺的研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110041727A (en) * | 2019-05-17 | 2019-07-23 | 石河子大学 | A kind of method of ultrasound assisted extraction beet red pigment |
| CN111480828A (en) * | 2020-04-24 | 2020-08-04 | 山东省蚕业研究所 | Method for co-extracting betacyanin and plant salt in suaeda salsa |
| CN111480828B (en) * | 2020-04-24 | 2022-10-25 | 山东省蚕业研究所 | Method for co-extracting betacyanin and plant salt in suaeda salsa |
| CN111925402A (en) * | 2020-08-03 | 2020-11-13 | 北京师范大学 | Method for separating and refining multiple betacyanins from suaeda salsa |
| CN111925402B (en) * | 2020-08-03 | 2022-07-05 | 北京师范大学 | Method for separating and refining multiple betacyanins from suaeda salsa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102219866B (en) | Method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp | |
| CN103333067B (en) | A kind of extracting method of high-purity chlorogenic acid | |
| CN107759555A (en) | A kind of Suaeda salsa anthocyanidin extracting method | |
| CN110684128B (en) | Method for extracting and refining polygonatum sibiricum polysaccharide | |
| CN101705275B (en) | Process for producing diosgenin and method for processing peltate yam after extracting same | |
| CN102491938B (en) | A kind of purification process of S-GI | |
| CN105193863A (en) | Preparation method of high-purity algal polyphenol | |
| CN105294790A (en) | Method for extracting high-purity steviol glycosides from stevia rebaudiana | |
| CN103087128A (en) | Method for extracting paeoniflorin from peony seed meal | |
| CN107594472A (en) | A kind of Suaeda salsa biogenic salt and Betacyanins combined extraction method | |
| CN104861079A (en) | Microwave assisted lentinan extraction equipment and technology | |
| CN101747388A (en) | Method for simultaneously extracting punicalagin and ellagic acid from pomegranate bark | |
| CN100522982C (en) | Production process for extracting tea saponin from tea-oil tree cake by using water as dissoluent | |
| CN102924544B (en) | Method for preparing stevioside and chlorogenic acid from stevia step by step | |
| CN104031158A (en) | Technique for extracting sulfate polysaccharide from Laminaria digitata | |
| CN107721966A (en) | A kind of Suaeda salsa anthocyanidin and biogenic salt combined extraction method | |
| CN104961839A (en) | Preparation method of specific pachyman formula granule | |
| CN103788152A (en) | Method for preparing geniposide in eucommia leaf | |
| CN109942657B (en) | Method for extracting dehydroepiandrosterone from sweet potatoes | |
| CN107594473A (en) | A kind of Suaeda salsa biogenic salt and Betacyanins integrated extraction technique | |
| CN107684568A (en) | A kind of method of the Hydrolysis kinetics high-purity silver flavone from ginkgo leaf | |
| CN105859916B (en) | A kind of preparation method of south No. 9 jerusalem artichoke inulins of jerusalem artichoke | |
| CN103772457B (en) | Method for preparing geniposidic acid in eucommia leaf | |
| CN107857825A (en) | A kind of extracting method of radix tetrastigme polysaccharide | |
| CN107760057A (en) | A kind of Suaeda salsa Betacyanins extracting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180119 |
|
| RJ01 | Rejection of invention patent application after publication |