CN103249835A - 猪肌抑素基因座位及其应用 - Google Patents
猪肌抑素基因座位及其应用 Download PDFInfo
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- CN103249835A CN103249835A CN2011800315627A CN201180031562A CN103249835A CN 103249835 A CN103249835 A CN 103249835A CN 2011800315627 A CN2011800315627 A CN 2011800315627A CN 201180031562 A CN201180031562 A CN 201180031562A CN 103249835 A CN103249835 A CN 103249835A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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- Biophysics (AREA)
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- Plant Pathology (AREA)
- Animal Husbandry (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Toxicology (AREA)
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- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
提供了一种猪肌抑素基因座位及其应用。还提供了一种表达盒，由启动子、外源基因和终止子组成：所述启动子为如下1）--3）中任一项所述的DNA分子：1）序列表的序列1自5’末端第2642-3778位核苷酸；2）序列表的序列1所示的核苷酸；3）在严格条件下与1）或2）限定的DNA序列杂交且具有相同功能的DNA分子。本发明的猪肌抑素基因座位为在该位点处进行基因打靶、转入并表达外源基因提供了有价值的基因资源。
Description
PCT国内申请，说明书已公开。
Claims (1)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2011/001119 WO2013003983A1 (zh) | 2011-07-06 | 2011-07-06 | 猪肌抑素基因座位及其应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103249835A true CN103249835A (zh) | 2013-08-14 |
| CN103249835B CN103249835B (zh) | 2015-05-20 |
Family
ID=47436435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180031562.7A Active CN103249835B (zh) | 2011-07-06 | 2011-07-06 | 猪肌抑素基因座位及其应用 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20140223592A1 (zh) |
| CN (1) | CN103249835B (zh) |
| WO (1) | WO2013003983A1 (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110157818B (zh) * | 2019-07-08 | 2023-04-25 | 云南东恒经贸集团猪育种有限公司 | 一种与猪眼肌高产性状相关的分子标记及其应用 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005820A2 (en) * | 1999-07-20 | 2001-01-25 | Pharmexa A/S | Method for down-regulating gdf-8 activity |
| CN1906211A (zh) * | 2003-11-28 | 2007-01-31 | 奥维塔有限公司 | 新的肌肉生长调节物 |
| CN101892264A (zh) * | 2010-05-28 | 2010-11-24 | 吉林大学 | 肌生成抑制素mstn基因敲除猪的建立 |
| CN102021201A (zh) * | 2010-09-06 | 2011-04-20 | 南京农业大学 | 一种肌肉生成抑制素基因敲除猪的制备方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050043234A1 (en) * | 1996-10-16 | 2005-02-24 | Deisher Theresa A. | Novel FGF homologs |
| US6469154B1 (en) * | 1999-05-21 | 2002-10-22 | The Regents Of The University Of California | Fluorescent protein indicators |
| EP1072680A1 (en) * | 1999-07-30 | 2001-01-31 | Pfizer Products Inc. | Myostatin regulatory region, nucleotide sequence determination and methods for its use |
| EP1593689A3 (en) * | 2000-01-18 | 2006-04-05 | Ovita Limited | Myostatin and mimetics thereof |
-
2011
- 2011-07-06 CN CN201180031562.7A patent/CN103249835B/zh active Active
- 2011-07-06 US US14/128,995 patent/US20140223592A1/en not_active Abandoned
- 2011-07-06 WO PCT/CN2011/001119 patent/WO2013003983A1/zh active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005820A2 (en) * | 1999-07-20 | 2001-01-25 | Pharmexa A/S | Method for down-regulating gdf-8 activity |
| CN1906211A (zh) * | 2003-11-28 | 2007-01-31 | 奥维塔有限公司 | 新的肌肉生长调节物 |
| CN101892264A (zh) * | 2010-05-28 | 2010-11-24 | 吉林大学 | 肌生成抑制素mstn基因敲除猪的建立 |
| CN102021201A (zh) * | 2010-09-06 | 2011-04-20 | 南京农业大学 | 一种肌肉生成抑制素基因敲除猪的制备方法 |
Non-Patent Citations (5)
| Title |
|---|
| A. STINCKENS ET AL.: "Characterization of the complete porcine MSTN gene and expression levels in pig breeds differing in muscularity", 《ANIMAL GENETICS》 * |
| KHANAM,T. ET AL.: "EU056363.1 Mus musculus transgenic enhanced green fluorescent protein (EGFP) mRNA, complete cds", 《GENBANK》 * |
| STINCKENS,A. ET AL.: "EF490988.1 Sus scrofa breed Meishan myostatin (MSTN) gene, complete cds", 《GENBANK》 * |
| 关学敏 等: "猪肌肉生长抑制素基因的研究进展", 《养猪》 * |
| 李鸿辉 等: "Myostatin基因打靶载体的构建及猪胎儿成纤维细胞Myostatin基因的敲除", 《生物技术通讯》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013003983A1 (zh) | 2013-01-10 |
| US20140223592A1 (en) | 2014-08-07 |
| CN103249835B (zh) | 2015-05-20 |
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