CN103211993A - Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis - Google Patents
Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis Download PDFInfo
- Publication number
- CN103211993A CN103211993A CN2013101482350A CN201310148235A CN103211993A CN 103211993 A CN103211993 A CN 103211993A CN 2013101482350 A CN2013101482350 A CN 2013101482350A CN 201310148235 A CN201310148235 A CN 201310148235A CN 103211993 A CN103211993 A CN 103211993A
- Authority
- CN
- China
- Prior art keywords
- weight portions
- herba
- chinese medicine
- medicine composition
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a traditional Chinese medicine composition for treating porcine transmissible gastroenteritis. The traditional Chinese medicine composition comprises the following active pharmaceutical ingredients: costustoot, natural indigo, common goldenrod, perfoliote knotweed herb, cochinchnese asparagus root, fresh bamboo sap, pterocephalus hookeri, coix seed, common St. Paulswort herb, entada phaseoloides, tree-of-heaven ailanthus bark, small centipeda herb, Japanese flowering fern rhizome, kudzuvine root, ovateleaf holly bark, south dodder seed, rhizome, abelmoschus manihot, amur corktree bark, Chinese taxillus twig, bistort rhizome, pilose Asiabell root and ash bark. The traditional Chinese medicine composition can overcome the shortcomings of the existing western medicine therapy and has the advantages of good efficacy against the porcine transmissible gastroenteritis, low toxicity and side effects, less residue, rich resources and low price.
Description
Technical field
The present invention relates to Chinese veterinarian's technical field, relate in particular to the Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine.
Background technology
Transmissible gastroenteritis of swine is a kind of acute infectious intestinal disease that is caused by coronavirus, propagate rapidly, the sickness rate height, mortality rate can reach 100% within 2 ages in week, even pilosity is educated bad after the piglet rehabilitation, growth retardation, virus mainly is present in the mucous membrane of small intestine and content of pig, excrete with fecal vomitus, infect through digestive tract, respiratory tract, primary disease is a feature with vomiting, diarrhoea, dehydration, morbidity season is obvious, be mainly in cold winter, spring, big piglets all can fall ill, and then death is seldom more than 2 all ages.Primary disease has taken place and has determined that its cause of disease is a coronavirus in the U.S. in reported first in 1945 in Doyle and Hutchings.Japan in 1956, nineteen fifty-seven, primary disease took place in Britain in succession, and primary disease has become a kind of global pig infectious disease at present.TaiWan, China was found transmissible gastroenteritis of swine first in 1958, be separated to transmissible gastroenteritis of swine in 1973 first in the China's Mainland.Up to now, the most of provinces and cities of China all have primary disease to take place.This disease outburst can cause the large quantities of death of newborn piglet, brings enormous economic loss to pig industry, is classified as the pig infectious disease that the category-B eqpidemic disease must be examined by World Organization for Animal Health's code.
This sick case is changed to the disease pig and becomes thin, and dehydration obviously; Gastric contains ziega, and gastric mucosa hyperemia, serous coat are hemorrhage; The small intestinal air-blowing, the visible diffusivity hyperemia of small bowel, hemorrhage, attenuation also lacks flexibility, mesenteric lymph node hyperemia, swelling, other do not have obviously observes pathological changes.As seen some case there is no other pathological changes except corpse dehydration, enteral full of liquid.
For transmissible gastroenteritis of swine; the also main at present immunotherapy that adopts; though it is strong that it has immunity; advantages such as the immunoprotection phase is long, but still there is remaining virulence in some weak malicious Seedlings, and indivedual Paracox still can cause the immunosuppressant of inoculation animal; the potential hazard of attenuated vaccine reversion still exists; on the other hand, because immune programme for children is misorient or route of inoculation is improper, also can cause immuning failure.Can produce certain curative effect though use symptomatic therapy, it is cured the symptoms, not the disease, and often causes this disease to be shown effect repeatedly.In addition, use antibiotic and easily produce drug resistance and residue problem, be unfavorable for the diagnosis and the treatment of disease, hindered the outlet of China's animal food.Veterinary clinic still lacks the medicine of safe and effective control transmissible gastroenteritis of swine at present, and therefore this medicine of development control is extremely urgent.
Chinese herbal medicine is China's tradition medical material, and it is little that it has toxic and side effects, residual few, aboundresources, advantage such as cheap a large number of experiments show that Chinese herbal medicine can not only influence duplicating of virus, can also by regulate immunity of organisms, non-specific antiinflammatory be brought into play its antivirus action.The cry of advocating pollution-free food after the China joined WTO is more and more higher, along with using the serious day by day of drug resistance problem that antibacterial brought, use herbal control animality disease and will cause more attention, add development of modern science and technology, science is selected the medicine prescription, and developing efficient, the safe Chinese herbal and crude drugs preparations of preventing and treating zoosis toxicity disease of more high quality will become possibility.
The present invention is exactly on this basis, proposes a kind of Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine, and it can overcome the deficiency of existing western medicine therapy, transmissible gastroenteritis of swine is had good curative effect, and toxic and side effects is little, and is residual few, aboundresources, cheap.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine, and the deficiency that it can overcome existing western medicine therapy has good curative effect to transmissible gastroenteritis of swine, toxic and side effects is little, residual few, aboundresources, cheap.
For solving the problems of the technologies described above, the invention provides a kind of Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine, its crude drug comprises the Radix Aucklandiae, Indigo Naturalis, Herba Solidaginis, Herba Polygoni cymosi, Radix Asparagi, fresh Succus Bambusae, Herba pterocephali, Semen Coicis, Herba Siegesbeckiae, Semen Entadae, Cortex Ailanthi, Herba Centipedae, Rhizoma Osmundae Japonicae, Radix Puerariae, Cortex Ilicis Rotundae, Semen Cuscutae, Rhizoma Polygonati, Flos abelmoschi manihot, Cortex Phellodendri, Herba Taxilli, Rhizoma Bistortae, Radix Codonopsis and Cortex Fraxini.
Wherein, the weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～30 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～70 weight portions, Herba Solidaginis 30 weight portions～40 weight portions, Herba Polygoni cymosi 25 weight portions～35 weight portions, Radix Asparagi 30 weight portions～40 weight portions, fresh Succus Bambusae 20 weight portions～30 weight portions, Herba pterocephali 15 weight portions～25 weight portions, Semen Coicis 20 weight portions～30 weight portion Herba Siegesbeckiaes, 10 weight portions～20 weight portion Semen Entadae, 20 weight portions～30 weight portions, Cortex Ailanthi 25 weight portions～35 weight portions, Herba Centipedae 30 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 20 weight portions～30 weight portions, Radix Puerariae 25 weight portions～35 weight portions, Cortex Ilicis Rotundae 20 weight portions～30 weight portions, Semen Cuscutae 35 weight portions～45 weight portions, Rhizoma Polygonati 50 weight portions～60 weight portions, Flos abelmoschi manihot 20 weight portions～30 weight portions, Cortex Phellodendri 40 weight portions～50 weight portions, Herba Taxilli 15 weight portions～25 weight portions, Rhizoma Bistortae 20 weight portions～30 weight portions, Radix Codonopsis 60 weight portions～70 weight portions and Cortex Fraxini 20 weight portions～30 weight portions.
Wherein, the weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～25 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～65 weight portions, Herba Solidaginis 30 weight portions～35 weight portions, Herba Polygoni cymosi 25 weight portions～30 weight portions, Radix Asparagi 30 weight portions～35 weight portions, fresh Succus Bambusae 20 weight portions～25 weight portions, Herba pterocephali 15 weight portions～20 weight portions, Semen Coicis 20 weight portions～25 weight portion Herba Siegesbeckiaes, 15 weight portions～20 weight portion Semen Entadae, 25 weight portions～30 weight portions, Cortex Ailanthi 30 weight portions～35 weight portions, Herba Centipedae 35 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 25 weight portions～30 weight portions, Radix Puerariae 30 weight portions～35 weight portions, Cortex Ilicis Rotundae 25 weight portions～30 weight portions, Semen Cuscutae 40 weight portions～45 weight portions, Rhizoma Polygonati 55 weight portions～60 weight portions, Flos abelmoschi manihot 25 weight portions～30 weight portions, Cortex Phellodendri 45 weight portions～50 weight portions, Herba Taxilli 20 weight portions～25 weight portions, Rhizoma Bistortae 25 weight portions～30 weight portions, Radix Codonopsis 65 weight portions～70 weight portions and Cortex Fraxini 25 weight portions～30 weight portions.
Wherein, the weight portion of each crude drug is respectively the Radix Aucklandiae 25 weight portions in the described Chinese medicine composition, Indigo Naturalis 65 weight portions, Herba Solidaginis 35 weight portions, Herba Polygoni cymosi 30 weight portions, Radix Asparagi 35 weight portions, fresh Succus Bambusae 25 weight portions, Herba pterocephali 20 weight portions, Semen Coicis 25 weight portion Herba Siegesbeckiaes 15 weight portion Semen Entadae 25 weight portions, Cortex Ailanthi 30 weight portions, Herba Centipedae 35 weight portions, Rhizoma Osmundae Japonicae 25 weight portions, Radix Puerariae 30 weight portions, Cortex Ilicis Rotundae 25 weight portions, Semen Cuscutae 40 weight portions, Rhizoma Polygonati 55 weight portions, Flos abelmoschi manihot 25 weight portions, Cortex Phellodendri 45 weight portions, Herba Taxilli 20 weight portions, Rhizoma Bistortae 25 weight portions, Radix Codonopsis 65 weight portions and Cortex Fraxini 25 weight portions.
Wherein, described Chinese medicine composition can be prepared into decoction liquor and injection.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, when described Chinese medicine composition is decoction liquor, concrete steps are: each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, is heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor of 1g/ml～2g/ml.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, and when described Chinese medicine composition was injection, concrete steps were: each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, hydrochloric acid solution with 4mol/l～6mol/l is regulated pH value to 2.0, and cold preservation 48h places precipitation, filter, filtrate is regulated pH value to 7.0 with 20% sodium hydroxide solution, adds to account for quality of liquid medicine 0.5% active carbon, keeps pH value 7.0, heat little boiling 15 minutes, cooling filters, and adds the injection water to 1000ml, canned, in 115 ℃ of sterilizations 30 minutes, cold preservation 48 hours filtered, packing promptly gets injection.
The present invention also provides above-mentioned Chinese medicine composition to be used for the treatment of application in the medicine of transmissible gastroenteritis of swine in preparation.
Beneficial effect of the present invention:
The Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine provided by the invention can overcome the deficiency of existing western medicine therapy, and transmissible gastroenteritis of swine is had good curative effect, and toxic and side effects is little, and is residual few, and aboundresources is cheap.
The specific embodiment
According to Chinese veterinarian's pathology theory, this disease is by due to the diseases caused by exogenous pathogenic factor epidemic disease caused by damp-heat pathogen poison, and sheerly gastrointestinal is damp and hot.Because stadium is different with pathology, the syndrome that is produced is variant.Sick exterior heat does not just separate gastrointestinal, for assorted gastrointestinal damp-heat syndrome is held in exterior heat under the arm, and middle after date, the damp-heat accumulation gastrointestinal, stomach-QI being unable to descend normally is so see vomiting, damp and hot criminal's spleen, then loss of appetite; Damp invasion of lower energizer, large intestine conduction and the pure and impure imbalance of intestinal secretion, water is wet can not to be separated, so rush down as annotating, short urination is few; Interior-heat is long-pending to be contained, so the happiness drink.
Therefore; treatment of the present invention is a principle with heat-clearing and toxic substances removing, dehumidifying antidiarrheal, benefiting qi and nourishing yin, antidiarrheal convergence, protection gastrointestinal mucosa; a kind of Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine is provided, and its crude drug comprises the Radix Aucklandiae, Indigo Naturalis, Herba Solidaginis, Herba Polygoni cymosi, Radix Asparagi, fresh Succus Bambusae, Herba pterocephali, Semen Coicis, Herba Siegesbeckiae, Semen Entadae, Cortex Ailanthi, Herba Centipedae, Rhizoma Osmundae Japonicae, Radix Puerariae, Cortex Ilicis Rotundae, Semen Cuscutae, Rhizoma Polygonati, Flos abelmoschi manihot, Cortex Phellodendri, Herba Taxilli, Rhizoma Bistortae, Radix Codonopsis and Cortex Fraxini.
Preferably, described Chinese medicine composition only is made of the above-mentioned raw materials medicine.
The weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～30 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～70 weight portions, Herba Solidaginis 30 weight portions～40 weight portions, Herba Polygoni cymosi 25 weight portions～35 weight portions, Radix Asparagi 30 weight portions～40 weight portions, fresh Succus Bambusae 20 weight portions～30 weight portions, Herba pterocephali 15 weight portions～25 weight portions, Semen Coicis 20 weight portions～30 weight portion Herba Siegesbeckiaes, 10 weight portions～20 weight portion Semen Entadae, 20 weight portions～30 weight portions, Cortex Ailanthi 25 weight portions～35 weight portions, Herba Centipedae 30 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 20 weight portions～30 weight portions, Radix Puerariae 25 weight portions～35 weight portions, Cortex Ilicis Rotundae 20 weight portions～30 weight portions, Semen Cuscutae 35 weight portions～45 weight portions, Rhizoma Polygonati 50 weight portions～60 weight portions, Flos abelmoschi manihot 20 weight portions～30 weight portions, Cortex Phellodendri 40 weight portions～50 weight portions, Herba Taxilli 15 weight portions～25 weight portions, Rhizoma Bistortae 20 weight portions～30 weight portions, Radix Codonopsis 60 weight portions～70 weight portions and Cortex Fraxini 20 weight portions～30 weight portions.
The weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～25 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～65 weight portions, Herba Solidaginis 30 weight portions～35 weight portions, Herba Polygoni cymosi 25 weight portions～30 weight portions, Radix Asparagi 30 weight portions～35 weight portions, fresh Succus Bambusae 20 weight portions～25 weight portions, Herba pterocephali 15 weight portions～20 weight portions, Semen Coicis 20 weight portions～25 weight portion Herba Siegesbeckiaes, 15 weight portions～20 weight portion Semen Entadae, 25 weight portions～30 weight portions, Cortex Ailanthi 30 weight portions～35 weight portions, Herba Centipedae 35 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 25 weight portions～30 weight portions, Radix Puerariae 30 weight portions～35 weight portions, Cortex Ilicis Rotundae 25 weight portions～30 weight portions, Semen Cuscutae 40 weight portions～45 weight portions, Rhizoma Polygonati 55 weight portions～60 weight portions, Flos abelmoschi manihot 25 weight portions～30 weight portions, Cortex Phellodendri 45 weight portions～50 weight portions, Herba Taxilli 20 weight portions～25 weight portions, Rhizoma Bistortae 25 weight portions～30 weight portions, Radix Codonopsis 65 weight portions～70 weight portions and Cortex Fraxini 25 weight portions～30 weight portions.
The weight portion of each crude drug is respectively the Radix Aucklandiae 25 weight portions in the described Chinese medicine composition, Indigo Naturalis 65 weight portions, Herba Solidaginis 35 weight portions, Herba Polygoni cymosi 30 weight portions, Radix Asparagi 35 weight portions, fresh Succus Bambusae 25 weight portions, Herba pterocephali 20 weight portions, Semen Coicis 25 weight portion Herba Siegesbeckiaes 15 weight portion Semen Entadae 25 weight portions, Cortex Ailanthi 30 weight portions, Herba Centipedae 35 weight portions, Rhizoma Osmundae Japonicae 25 weight portions, Radix Puerariae 30 weight portions, Cortex Ilicis Rotundae 25 weight portions, Semen Cuscutae 40 weight portions, Rhizoma Polygonati 55 weight portions, Flos abelmoschi manihot 25 weight portions, Cortex Phellodendri 45 weight portions, Herba Taxilli 20 weight portions, Rhizoma Bistortae 25 weight portions, Radix Codonopsis 65 weight portions and Cortex Fraxini 25 weight portions.
Described Chinese medicine composition can be prepared into decoction liquor and injection.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, when described Chinese medicine composition is decoction liquor, concrete steps are: each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, is heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor of 1g/ml～2g/ml.
The present invention also provides the using method of this decoction liquor, and 30 ages in days gavage 10ml～20ml with every of interior piglet at every turn, and the above person of 30 ages in days gavages 50ml～80ml at every turn, every day 2 times～3 times.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, and when described Chinese medicine composition was injection, concrete steps were: each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, hydrochloric acid solution with 4mol/l～6mol/l is regulated pH value to 2.0, and cold preservation 48h places precipitation, filter, filtrate is regulated pH value to 7.0 with 20% sodium hydroxide solution, adds to account for quality of liquid medicine 0.5% active carbon, keeps pH value 7.0, heat little boiling 15 minutes, cooling filters, and adds the injection water to 1000ml, canned, in 115 ℃ of sterilizations 30 minutes, cold preservation 48 hours filtered, packing promptly gets injection.
Injection provided by the invention can adopt the mode of intramuscular injection or intravenous drip to inject.
The present invention also provides the using method of this decoction liquor, and 30 ages in days are with every per injection 1ml～1.5ml of interior piglet, the above person's per injection of 30 ages in days 2ml～3ml, every day 2 times.
The Radix Aucklandiae: acrid in the mouth, hardship, warm in nature, go into spleen, stomach, large intestine, gallbladder meridian, week analgesic effect is arranged, all gas of energy lifting, it is stagnant to be longer than capable the intestines and stomach again, leads the heap soil or fertilizer over and around the roots of three warmers; The card of all mechanism of qi retardances as the intestines and stomach stagnation of QI, is gone up and to be vomiting, in be distension and fullness in the abdomen, down for having loose bowels, also can be used for the damp and hot stagnant dysentery of the intestines and stomach, symptoms such as tenesmus have inhibitory action to transmissible gastroenteritis, Bacillus typhi, bacillus subtilis, dysentery bacterium.
Indigo Naturalis: salty in the mouth, cold in nature, go into Liver Channel, have the effect of heat-clearing and toxic substances removing, cooling blood for hemostasis, purging liver-fire, be used for epidemic febrile disease and send out cards such as all cards of pyretic toxicity, liver-fire invading the lung, cough chest pain, sputum mixed with blood, hyperpyrexia infantile convulsion, convulsion with spasms such as speckle, haematemesis, laryngopharynx swelling and pain, herpes eczema.
Herba Solidaginis: latin name Solidaginis Herba, acrid in the mouth, hardship, cool in nature, return lung, Liver Channel, have the effect of heat-clearing and toxic substances removing, dispelling wind and heat pathogens, cure mainly cards such as sore throat, laryngopharynx swelling and pain, anemopyretic cold.
Herba Polygoni cymosi: sour in the mouth, cold nature is returned lung, urinary bladder channel, has the effect of heat-clearing and toxic substances removing, inducing diuresis to remove edema, cough-relieving, cures mainly laryngopharynx swelling and pain, cough due to lung-heat, children's's pertussis, edema oliguria, damp-heat dysentery, cards such as eczema.
Radix Asparagi: sweet in the mouth, hardship, cold in nature, return lung, kidney channel, have nourishing YIN to relieve dryness, the effect that lung heat clearing is promoted the production of body fluid cures mainly cough caused by dryness-heat, deficiency of YIN chronic cough, hyperactivity of fire caused by deficiency of YIN, the laryngopharynx swelling and pain of seminal emission, premature ejaculation and hyperactivity of deficient fire that kidney essense is not solid, calentura Tianjin wound is thirsty, the interior-heat cards such as dryness of the intestine constipation of quenching one's thirst.
Fresh Succus Bambusae: sweet in the mouth, cold in nature, GUIXIN, lung, Liver Channel, the effect of have heat clearing away, eliminating phlegm, moisturize, quenching the thirst cures mainly the calentura agitation, card such as the recurrence caused by overexertion of quenching one's thirst, wind-phlegm, deficiency-phlegm, apoplectic aphasia.
Herba pterocephali: latin name Pterocephali Herba, bitter in the mouth, cold in nature, have detoxifcation and remove pestilence, clearing away heat to cure dysentery, cards such as arthralgia aggravated by cold dispel the wind.
Semen Coicis: cool in nature, sweet in the mouth, light is returned spleen, stomach, lung meridian.Have the function of spleen invigorating eliminating dampness by diuresis, clearing away heat and discharging pus, eliminating impediment, diuretic, be used for weakness of the spleen and stomach, loose stool diarrhoea, or vaginal discharge diseases of women, the insufficiency of the spleen leading to overabundance of dampness edema, dysuria, or beriberi swells and ache; Damp and hot arthralgia pain, contracture of the limbs, miserable pain; Lung abscess cough and spit purulent sputum, or enterodynia contracture stomachache.
Herba Siegesbeckiae: latin name Siegesbeckiae Herba, flavor is cold, hot, bitter, returns Liver Channel, kidney channel, has wind-damp dispelling, sharp joint, the antidotal effect cures mainly cards such as rheumatic arthralgia, muscles and bones are unable, soreness of the waist and knees, quadriplegia, hemiplegia, rubella eczema.
Semen Entadae: mildly bitter flavor, liver, spleen, stomach, kidney channel are returned in cold, have QI invigorating and enrich blood, invigorating the stomach and promoting digestion, remove the wind pain relieving, the effect of strong muscle os osseum cures mainly the blood and slime deficiency, pale complexion, myasthenia of limbs, epigastric pain, indigestion and loss of appetite lack of appetite, the rheumatism limbs joint soft pain of withering, cards such as hyposexuality.
Cortex Ailanthi: bitter in the mouth, puckery, cold in nature, go into large intestine, stomach, Liver Channel, have heat clearing and damp drying, the astringing intestine to stop diarrhea leukorrhagia stopping, hemostasis, parasiticidal effect is used for damp-heat dysentery, the chronic diarrhea chronic dysentery, metrorrhagia is had blood in stool, hemorrhoid hematochezia, leukorrhagia, cards such as discharging fresh blood stool.
Herba Centipedae: latin name Centipedae Hebra, acrid in the mouth, warm in nature, go into lung, Liver Channel, have dispel the wind cold, clearing the nasal passage, eliminating phlegm and stopping cough, the effect of dissipating blood stasis for subsidence of swelling is used for exterior syndrome due to wind-cold, nasal obstruction watery nasal discharge and nasal sinusitis, cough with copious phlegm, pertussis, cards such as sore, carbuncle and painful swelling.
Rhizoma Osmundae Japonicae: bitter in the mouth, cold nature is returned spleen, and the stomach warp has heat-clearing and toxic substances removing, hemostasis by removing blood stasis, parasiticidal effect is used for influenza; Epidemic encephalitis; Encephalitis b parotitis; The carbuncle pyogenic infections from tumour or sore; Measles; Chickenpox; Dysentery; Spit blood; Epistaxis; Have blood in stool; Metrorrhagia; Leukorrhagia; Pinworm; Cards such as cestode.
Radix Puerariae: sweet in the mouth, suffering, cool in nature, go into spleen, stomach warp, the inspiration gastric qi is arranged, clearing away heat and promoting production of body fluid, expelling pathogenic factors from muscles is delivered, and gives birth to sun, oozes the effect of rash, is mainly used in fever caused by exogenous pathogens, headache, stiff nape and back, calentura is thirsty, quenches one's thirst; Diarrhea due to hypofunction of the spleen, cards such as clear YANG failing to ascend.
Cortex Ilicis Rotundae: cold in nature, bitter in the mouth is gone into lung, liver, large intestine channel, has heat-clearing and toxic substances removing, dampness removing, analgesic effect, cures mainly cards such as cold, fever, laryngopharynx swelling and pain, stomachache, administration's diarrhea due to damp pathogen rush down, jaundice, dysentery, traumatic injury, rheumatic arthralgia, eczema, furuncle.
Semen Cuscutae: sweet in the mouth, suffering, property is flat, goes into the liver,kidney,spleen warp, the kidney invigorating and essence nourishing is arranged, and the effect of nourishing the liver to improve visual acuity cures mainly the sexual impotence of insufficiency of kidney-YANG, the seminal emission premature ejaculation, frequent micturition, dizziness and tinnitus, the pain in the lumbar region of suffering from a deficiency of the kidney, married woman's leukorrhagia, nausea are not solid, dim-sighted, the blurring of vision of the binocular of both liver and kidney deficiency, deficiency of liver-blood, the loose stool stomachache of asthenia of both the spleen and kidney, cards such as aqueous-grainy diarrhea.
Rhizoma Polygonati: latin name Polygonati Rhizoma, sweet in the mouth, property is flat, go into lung, spleen, kidney channel, has the yin nourishing of fostering the spirit of nobility, spleen invigorating lung moistening, the effect of enriching kidney essence, be used for the taste deficiency of qi and yin, lack of appetite is unable, lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, quenching one's thirst of interior-heat caused by deficiency of YIN, cards such as deficiency of kidney essence, impotence and seminal emission, dizziness and tinnitus, blurred vision dim eyesight.
Flos abelmoschi manihot: latin name Abelmoschus manihot (L.) Medic, sweet in the mouth, suffering, cool in nature, GUIXIN; Kidney; Urinary bladder channel has inducing diuresis for treating stranguria syndrome; Invigorate blood circulation; Hemostasis; The effect of subduing swelling and detoxicating, main stranguria; Spit blood; Epistaxis; Metrorrhagia; Placenta retention; Carbuncle sore tumefacting virus; Burn due to hot liquid or fire.
Cortex Phellodendri: bitter in the mouth, cold in nature, go into kidney, bladder, large intestine channel, heat clearing and damp drying, eliminating fire and detoxication removes the hectic fever due to YIN-deficiency clearind deficient heat.Be used for damp-heat dysentery, jaundice, leukorrhagia, pyretic stranguria, beriberi, flaccidity is warded off, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema pruritus.The stir-baked CORTEX PHELLODENDRI with salt solution nourishing YIN to lower pathogenic fire.Be used for hyperactivity of fire caused by deficiency of YIN, the night sweat hectic fever due to YIN-deficiency.
Herba Taxilli: bitter in the mouth, sweet, property is flat, goes into liver, kidney channel, effect with invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling, antiabortive unit is used for the soreness of waist pain due to the deficiency of the liver and kindey, and the muscles and bones flaccidity is soft, the foot and knee being feeble, deficiency of the liver and kindey, essence and blood is empty to lose the frequent fetal movement of liability to abortion, cards such as waist abdomen pain.
Rhizoma Bistortae: bitter in the mouth, puckery, cold nature is returned lung, liver, large intestine channel, has heat-clearing and toxic substances removing, detumescence, the hemostatic effect is used for treatment of diseases such as enteritis, dysentery, hepatitis, bleeding hemorrhoids, chamber inflammation, pharyngolaryngitis.
Radix Codonopsis: latin name Codonopsis Radix, sweet in the mouth, property is flat, go into lung, spleen channel, the QI invigorating strengthening the spleen is arranged, the effect of nourishing blood to promote the production of body fluid, cure mainly deficiency of both the splenic and pulmonary QI or QI and blood deficiency fatigue and weakness, breathe hard, cards such as cough spontaneous perspiration, Radix Codonopsis is longer than the spleen reinforcing nourishing the stomach, transfers regulating the function of middle-JIAO, has the effect of nourishing blood concurrently, its property is flat, spleen invigorating fortune and not dry; Flavour is cloudy and not wet, can improve modulation on immune status, and disease resistance enhancemen, facilitating digestion absorb, and improve metabolism, promote the absorption of intestinal to nutrient substance.
Cortex Fraxini: bitter in the mouth, puckery, cold in nature, go into liver, gallbladder, large intestine channel, have that dampness-eliminating and dysentery-stopping, liver heat removing purge heat, the effect of relieving cough and asthma, be mainly used in the red white dysentery that the damp and hot pent-up of treatment, gastrointestinal conduction are obstructed, tenesmus; The leucorrhea with red and white discharge of damp invasion of lower energizer; The conjunctival congestion and swelling pain of flaming up of liver-fire, order is given birth to nebula film and lung-heat cough with asthma excessive phlegm, symptoms such as asthma, Cortex Fraxini has stronger inhibitory action to dysentery bacterium, alpha streptococcus etc., and the effect that suppresses enterokinesia, link enterospasm is arranged.
Below adopt embodiment to describe embodiments of the present invention in detail, how the application technology means solve technical problem to the present invention whereby, and the implementation procedure of reaching technique effect can fully understand and implements according to this.
Embodiment 1 decoction liquor 1
With Radix Aucklandiae 25g, Indigo Naturalis 65g, Herba Solidaginis 35g, Herba Polygoni cymosi 30g, Radix Asparagi 35g, fresh Succus Bambusae 25g, Herba pterocephali 20g, Semen Coicis 25g Herba Siegesbeckiae 15g Semen Entadae 25g, Cortex Ailanthi 30g, Herba Centipedae 35g, Rhizoma Osmundae Japonicae 25g, Radix Puerariae 30g, Cortex Ilicis Rotundae 25g, Semen Cuscutae 40g, Rhizoma Polygonati 55g, Flos abelmoschi manihot 25g, Cortex Phellodendri 45g, Herba Taxilli 20g, Rhizoma Bistortae 25g, Radix Codonopsis 65g and Cortex Fraxini 25g mix, add 4.5kg water, be heated to fluidized state with intense fire, subsequently with slow fire boiling 4 hours, decoction liquor is filtered, filtering residue adds the water of 3kg once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 3 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor 1 of 1g/ml.
Embodiment 2 decoction liquor 2
With Radix Aucklandiae 20g, Indigo Naturalis 60g, Herba Solidaginis 30g, Herba Polygoni cymosi 25g, Radix Asparagi 30g, fresh Succus Bambusae 20g, Herba pterocephali 15g, Semen Coicis 20g Herba Siegesbeckiae 20g Semen Entadae 30g, Cortex Ailanthi 35g, Herba Centipedae 40g, Rhizoma Osmundae Japonicae 30g, Radix Puerariae 30 weight portions～35g, Cortex Ilicis Rotundae 30g, Semen Cuscutae 45g, Rhizoma Polygonati 60g, Flos abelmoschi manihot 30g, Cortex Phellodendri 50g, Herba Taxilli 25g, Rhizoma Bistortae 30g, Radix Codonopsis 70g and Cortex Fraxini 30g mix, add 4.5kg water, be heated to fluidized state with intense fire, subsequently with slow fire boiling 4 hours, decoction liquor is filtered, filtering residue adds the water of 3kg once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 3 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor 2 of 2g/ml.
Embodiment 3 decoction liquor 3
With Radix Aucklandiae 30g, Indigo Naturalis 70g, Herba Solidaginis 40g, Herba Polygoni cymosi 35g, Radix Asparagi 40g, fresh Succus Bambusae 30g, Herba pterocephali 25g, Semen Coicis 30g Herba Siegesbeckiae 10g Semen Entadae 20g, Cortex Ailanthi 25g, Herba Centipedae 30g, Rhizoma Osmundae Japonicae 20g, Radix Puerariae 25g, Cortex Ilicis Rotundae 20g, Semen Cuscutae 35g, Rhizoma Polygonati 50g, Flos abelmoschi manihot 20g, Cortex Phellodendri 40g, Herba Taxilli 15g, Rhizoma Bistortae 20g, Radix Codonopsis 60g and Cortex Fraxini 20g mix, add 4.5kg water, be heated to fluidized state with intense fire, subsequently with slow fire boiling 4 hours, decoction liquor is filtered, filtering residue adds the water of 3kg once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 3 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor 3 of 1g/ml.
Embodiment 4 injection 1
With Radix Aucklandiae 25g, Indigo Naturalis 65g, Herba Solidaginis 35g, Herba Polygoni cymosi 30g, Radix Asparagi 35g, fresh Succus Bambusae 25g, Herba pterocephali 20g, Semen Coicis 25g Herba Siegesbeckiae 15g Semen Entadae 25g, Cortex Ailanthi 30g, Herba Centipedae 35g, Rhizoma Osmundae Japonicae 25g, Radix Puerariae 30g, Cortex Ilicis Rotundae 25g, Semen Cuscutae 40g, Rhizoma Polygonati 55g, Flos abelmoschi manihot 25g, Cortex Phellodendri 45g, Herba Taxilli 20g, Rhizoma Bistortae 25g, Radix Codonopsis 65g and Cortex Fraxini 25g mix, add 4.5kg water, be heated to fluidized state with intense fire, subsequently with slow fire boiling 4 hours, decoction liquor is filtered, filtering residue adds the water of 3kg once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 3 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, hydrochloric acid solution with 5mol/l is regulated pH value to 2.0, cold preservation 48h, place precipitation, filter, filtrate is regulated pH value to 7.0 with 20% sodium hydroxide solution, adds to account for quality of liquid medicine 0.5% active carbon, keeps pH value 7.0, heat little boiling 15 minutes, cooling filters, and adds the injection water to 1000ml, canned, in 115 ℃ of sterilizations 30 minutes, cold preservation 48 hours filtered, packing promptly gets injection 1.
The inhibitory action of 5 pairs of transmissible gastro-enteritis viruss of embodiment
1. reagent
The DMEM culture fluid, U.S. Gibco company product.After being dissolved in water, add 8% hyclone, NaHCO
3And conventional two anti-, make separately that concentration is respectively 10mmol/L, 0.37%, 100U/mL penicillin and 100U/mL streptomycin after regulating pH and being 7.0～7.2, are settled to 1000mL, and with the cellulose microporosity filter membrane filtration sterilization of 0.22 μ m, it is standby to be sub-packed in-20 ℃ of preservations; Hyclone (Qingdao Technological University); The packing of the magnificent company of trypsin Amresco); Crystal violet, analytical pure (Chinese oceangoing voyage chemical plant).All the other reagent are commercially available analytical pure.
2. instrument
96 well culture plates (Becton Dickinsen Labware company); The medical clean work station of YJ21450 (Suzhou Decontamination Equipment Plant); CO
2Incubator (Incubator IF241, Japan); Inverted phase contrast microscope (optical instrument factory, Chongqing); Full-automatic microplate reader (U.S. Bio2Rad Bole company).
3. virus and cell
(swinetesticle, ST) cell provides by Shanghai animal and veterinary institute for transmissible gastro-enteritis virus (TGEV), pig testis.
4. medicine
The injection 1 of the embodiment of the invention 4 preparations.
5. method
5.1ST cell recovery and cultivation
From-70 ℃ cryogenic refrigerator, take out the frozen pipe that contains the ST cell, put constantly jolting in 37 ℃ of water-baths immediately, the cell of frozen pipe is thawed in 30s.With uncap after 75% the wipes of alcohol wash disinfection, sucking-off cell suspension, also with the DMEM culture fluid dilution that contains 12% hyclone, the centrifugal 8min of 1500r/min abandons supernatant to the centrifuge tube of packing into, add and state culture fluid and suspend again, change in the culture bottle of 100mL, grow in the DMEM culture fluid that contains 12% hyclone, at 37 ℃, saturated humidity contains 5%CO
2Incubator in leave standstill cultivation, change culture fluid next day and continue to cultivate.Behind the 4d, with the ST cell in the conventional digestion of the trypsin culture bottle, with the DMEM inoculum preparation 2 * 10 that contains 12% hyclone
6The suspension inoculation of individual/mL on 96 well culture plates, every hole 0.4mL.
5.2 the mensuration of 1 pair of ST cytotoxicity of injection concentration
0.5ml injection 1 is joined in the cultivation plate hole, observe each effect of porocyte pathological changes (cytophathogenic effect, CPE) situation behind the cultivation 3d.Anosis become "-"; CPE＜25% pathological changes is "+"; 25%～50% pathological changes is " ++ "; 50%～75% pathological changes is " +++"; CPE〉75% pathological changes is " ++ ++ ".
5.3TGEV median infective dose (TCID
50) mensuration
Pressing CPE terminal point dilution method measures.With the DMEM culture fluid TGEV is progressively diluted from 10 by 10 times
-1To 10
-10The concentration of each gradient joins in 96 well culture plates that cover with monolayer ST cell then, at 37 ℃, and 5%CO
2And Mixed culture under the saturation temperature, observe CPE behind the 3d and reach 50% TGEV concentration value, be defined as TCID
50
The body outer suppressioning test of 1 couple of TGEV of 6 Chinese medicine injections of the present invention
6.1 the protective effect of the ST cell that 1 couple of TGEV of Chinese medicine injection of the present invention infects
On 96 orifice plates, inoculate 2 * 10
5The ST cell 0.3mL of individual/mL, treat that it grows into monolayer after, every hole adds TGEV virus liquid (100TCID
50/ mL) 0.1mL.After hatching 2h, 1,37 ℃ of the injection of adding 0.5ml embodiment 4 preparations, 5%CO
2Cultivate, each concentration is done 4 multiple holes, and experiment repeats 1 time.The CPE that carries out the ST cell behind the 3d earlier observes, and judges the CPE grade according to the cell number that occurs shrinking.Then, the supernatant in every hole is abandoned in suction, with 37 ℃, after the PBS washed twice of pH7.6, add 0.2% crystal violet solution 0.1mL dyeing 5min, reuse PBS washing 5 times, add 1% sodium dodecyl sulfate solution 0.1mL, 37 ℃ of insulation 0.5h make the thorough cracking of cell, put on the full-automatic microplate reader, survey the A value down in 630nm.
6.2 the direct effect of 1 couple of TGEV of Chinese medicine injection of the present invention
Injection 1 and TGEV virus liquid (100TCID with 0.5mL embodiment 4 preparations
50/ mL) 0.1mL mixes the back and hatches 2h in 37 ℃, and then be inoculated in the ST cell of 0.3mL, 37 ℃, 5%CO
2Cultivate.Observe the CPE degree behind the 3d and in the mensuration A of 630nm place value.
6.3 1 couple of TGEV of Chinese medicine injection of the present invention infects the preventive protection effect of ST cell
With the injection 1 of 0.5mL embodiment 4 preparation and ST Cell sap 0.3mL at 96 well culture plates, at 37 ℃, 5%CO
2After cultivating 4h, add TGEV virus liquid (100TCID
50/ mL) 0.1mL is hatched 2h, changes and keeps liquid, continues to cultivate 3d.Observe the A value of the CPE and the 630nm under the violet staining in each hole with method.
6.4 the protective effect of different phase behind 1 pair of ST cell infection of Chinese medicine injection of the present invention TGEV
Inoculation TGEV virus liquid (100TCID in the hole of containing ST Cell sap 0.3mL
50/ mL) 0.1mL, 1,2,4,6 and 8h after add the Chinese medicine injection 1 of the 0.1mL embodiment of the invention 4 preparations respectively, making its final concentration in the hole is 54.50 μ mol/L, and establishes the viral infection contrast and normal cell contrasts, at 37 ℃, 5%CO
2Continue to cultivate 3d.
Observe behind the 3d CPE of cell and the viable count situation of violet staining in each hole.
7. statistical analysis
Adopt SPSS13.0 software to carry out statistical procedures, data with
Expression, measurement data is checked with t, enumeration data χ
2Check.
8. result
8.1 the toxic action of 1 pair of ST cell of injection
Liquid group no significant difference is kept in the effect of 1 pair of ST cell of injection and cultivation, and the form of ST cell does not all have obvious change.
8.2TCID
50Mensuration
When the dilution factor of TGEV virus liquid is 10
-7The time, the ST cell in 6 holes has the half cell to produce pathological changes, and atrophy occurs, come off, so TCID
50Be defined as 10
-7In following each test, all adopt 100TCID
50The virus quantity of/mL.
8.3 the body outer suppressioning test of 1 couple of TGEV of injection
8.3.1 the protective effect of the ST cell that 1 couple of TGEV of injection infects
Table 1
| Group | A 630 | CPE |
| Blank group | 1.257±0.145 | - |
| The TGEV group | 0.091±0.016 | ++++ |
| Chinese drug-treated group | 1.573±0.122 | - |
As can be seen from Table 1, the injection 1 that adds 4 preparations of the 0.5ml embodiment of the invention behind the virus absorption onto cell 2h can improve the survival number of ST cell significantly, demonstrates anti-TGEV effect, and is good dependency.CPE observes and also shows same result, almost is to protect fully to the ST cell.
8.3.2 the direct effect of 1 couple of TGEV of injection
Table 2
| Group | A 630 | CPE |
| Blank group | 1.399±0.068 | - |
| The TGEV group | 0.268±0.019 | ++++ |
| Chinese drug-treated group | 1.492±0.089 | - |
As can be seen from Table 2, the ST cell survivaling number showed increased in each hole shows under injection 1 of the present invention and the TGEV Mixed culture, can reduce its virus virulence; Find that simultaneously injection 1 acts on TGEV earlier, can make the survival number of ST cell improve better effects if.
8.3.3 1 couple of TGEV of injection infects the preventive protection effect of ST cell
Table 3
| Group | A 630 | CPE |
| Blank group | 1.151±0.059 | - |
| The TGEV group | 0.192±0.025 | ++++ |
| Chinese drug-treated group | 0.993±0.040 | - |
As can be seen from Table 3, Chinese medicine injection 1 provided by the invention can make the ST cell survivaling number showed increased in each hole, does not see the generation of CPE, shows the attack that can make the ST cell avoid TGEV virus, suppresses the breeding of virus.
8.3.4 the protective effect of different phase behind 1 pair of ST cell infection of injection TGEV
Administration in 1～8h after TGEV infects the ST cell, injection 1 all can obviously suppress the breeding of virus, but postponement along with administration time, cell survivaling number decreases, A value during 6～8h is compared with 1h, significant minimizing is arranged, illustrate that virus and bonded time of cell are long more, Chinese medicine composition antivirus action of the present invention is weak more.
Embodiment 6 model cases 1
On JIUYUE 21st, 2008, certain village Lee's a body weight 150kg sow due to farrow, and the morning, food was normal, do not eat afternoon flatly, vomiting, and about 8 as a child began diarrhoea, feces is spurting, and yellow skin fishy smell is unpleasant, and this moment, sow can also eat a spot of food, 40 ℃ of body temperature, the Chinese medicine composition decoction liquor 1 that adopts the embodiment of the invention 1 to prepare, the morning, noon and afternoon respectively gavage 1 time, each 70ml, the 2nd day afternoon, stool can be in heaps, gavages two days again, stool is normal, recovery from illness.
Embodiment 7 model cases 2
On July 3rd, 2011, certain village Wu comes to go to a doctor.The main suit: bought 40 of piglets on June 23rd, 2011, when at a time when the rainy day, the colony house humidity, suffering from diarrhoea in 1 week back, fails to respond to any medical treatment with dipyrone, compound aminophenazone, penicillin, chloromycetin, moroxydine etc.The person of having a delicate constitution is astasia, and extremity are ice-cold.Through looking into, examine and be transmissible gastroenteritis, control Chinese medicine composition decoction liquor 1 with embodiment 1 preparation, through controlling 3d, the morning, noon and afternoon respectively gavage 1 time, each 20ml, diarrhoea all stops, and all diseases disappear, and spirit, appetite recover normal.
Embodiment 8 model cases 3
On JIUYUE 18th, 2011, and the piglet of 7 about 20kg of certain village's relative is gone to a doctor because of the food of acutely vomiting and diarrhoea, tremble, give up.See disease: tongue is red slightly, and yellow and thin fur trembles.Examine and be transmissible gastroenteritis, control Chinese medicine composition composition injection 1 with embodiment 4 preparations, every day 1 time, each 1.5ml, through controlling 5d, diarrhoea all stops, and all diseases disappear, and spirit, appetite recover normal.
This intellectual property of primary enforcement that all are above-mentioned is not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (8)
1. Chinese medicine composition that is used for the treatment of transmissible gastroenteritis of swine, it is characterized in that: the crude drug of described Chinese medicine composition comprises the Radix Aucklandiae, Indigo Naturalis, Herba Solidaginis, Herba Polygoni cymosi, Radix Asparagi, fresh Succus Bambusae, Herba pterocephali, Semen Coicis, Herba Siegesbeckiae, Semen Entadae, Cortex Ailanthi, Herba Centipedae, Rhizoma Osmundae Japonicae, Radix Puerariae, Cortex Ilicis Rotundae, Semen Cuscutae, Rhizoma Polygonati, Flos abelmoschi manihot, Cortex Phellodendri, Herba Taxilli, Rhizoma Bistortae, Radix Codonopsis and Cortex Fraxini.
2. Chinese medicine composition as claimed in claim 1 is characterized in that: the weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～30 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～70 weight portions, Herba Solidaginis 30 weight portions～40 weight portions, Herba Polygoni cymosi 25 weight portions～35 weight portions, Radix Asparagi 30 weight portions～40 weight portions, fresh Succus Bambusae 20 weight portions～30 weight portions, Herba pterocephali 15 weight portions～25 weight portions, Semen Coicis 20 weight portions～30 weight portion Herba Siegesbeckiaes, 10 weight portions～20 weight portion Semen Entadae, 20 weight portions～30 weight portions, Cortex Ailanthi 25 weight portions～35 weight portions, Herba Centipedae 30 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 20 weight portions～30 weight portions, Radix Puerariae 25 weight portions～35 weight portions, Cortex Ilicis Rotundae 20 weight portions～30 weight portions, Semen Cuscutae 35 weight portions～45 weight portions, Rhizoma Polygonati 50 weight portions～60 weight portions, Flos abelmoschi manihot 20 weight portions～30 weight portions, Cortex Phellodendri 40 weight portions～50 weight portions, Herba Taxilli 15 weight portions～25 weight portions, Rhizoma Bistortae 20 weight portions～30 weight portions, Radix Codonopsis 60 weight portions～70 weight portions and Cortex Fraxini 20 weight portions～30 weight portions.
3. Chinese medicine composition as claimed in claim 1 or 2 is characterized in that: the weight portion of each crude drug is respectively the Radix Aucklandiae 20 weight portions～25 weight portions in the described Chinese medicine composition, Indigo Naturalis 60 weight portions～65 weight portions, Herba Solidaginis 30 weight portions～35 weight portions, Herba Polygoni cymosi 25 weight portions～30 weight portions, Radix Asparagi 30 weight portions～35 weight portions, fresh Succus Bambusae 20 weight portions～25 weight portions, Herba pterocephali 15 weight portions～20 weight portions, Semen Coicis 20 weight portions～25 weight portion Herba Siegesbeckiaes, 15 weight portions～20 weight portion Semen Entadae, 25 weight portions～30 weight portions, Cortex Ailanthi 30 weight portions～35 weight portions, Herba Centipedae 35 weight portions～40 weight portions, Rhizoma Osmundae Japonicae 25 weight portions～30 weight portions, Radix Puerariae 30 weight portions～35 weight portions, Cortex Ilicis Rotundae 25 weight portions～30 weight portions, Semen Cuscutae 40 weight portions～45 weight portions, Rhizoma Polygonati 55 weight portions～60 weight portions, Flos abelmoschi manihot 25 weight portions～30 weight portions, Cortex Phellodendri 45 weight portions～50 weight portions, Herba Taxilli 20 weight portions～25 weight portions, Rhizoma Bistortae 25 weight portions～30 weight portions, Radix Codonopsis 65 weight portions～70 weight portions and Cortex Fraxini 25 weight portions～30 weight portions.
4. as the described Chinese medicine composition of claim 1 to 3, it is characterized in that: the weight portion of each crude drug is respectively the Radix Aucklandiae 25 weight portions in the described Chinese medicine composition, Indigo Naturalis 65 weight portions, Herba Solidaginis 35 weight portions, Herba Polygoni cymosi 30 weight portions, Radix Asparagi 35 weight portions, fresh Succus Bambusae 25 weight portions, Herba pterocephali 20 weight portions, Semen Coicis 25 weight portion Herba Siegesbeckiaes 15 weight portion Semen Entadae 25 weight portions, Cortex Ailanthi 30 weight portions, Herba Centipedae 35 weight portions, Rhizoma Osmundae Japonicae 25 weight portions, Radix Puerariae 30 weight portions, Cortex Ilicis Rotundae 25 weight portions, Semen Cuscutae 40 weight portions, Rhizoma Polygonati 55 weight portions, Flos abelmoschi manihot 25 weight portions, Cortex Phellodendri 45 weight portions, Herba Taxilli 20 weight portions, Rhizoma Bistortae 25 weight portions, Radix Codonopsis 65 weight portions and Cortex Fraxini 25 weight portions.
5. as the described Chinese medicine composition of claim 1 to 4, it is characterized in that: described Chinese medicine composition can be prepared into decoction liquor and injection.
6. the preparation method of the described Chinese medicine composition of claim 1 to 5, it is characterized in that: when described Chinese medicine composition is decoction liquor, concrete steps are that each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, is heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, concentrate and remove part water, obtaining crude drug concentration is the Chinese medicine composition decoction liquor of 1g/ml～2g/ml.
7. the preparation method of the described Chinese medicine composition of claim 1 to 5 is characterized in that: when described Chinese medicine composition was injection, concrete steps were that each raw material medicaments in part by weight is mixed, adding is with respect to 5～7 times of water of mixture, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, filtering residue adds the water with respect to 3～5 times in mixture once more, be heated to fluidized state with intense fire, subsequently with slow fire boiling 2～4 hours, decoction liquor is filtered, the filtrate of twice acquisition is merged, hydrochloric acid solution with 4mol/l～6mol/l is regulated pH value to 2.0, and cold preservation 48h places precipitation, filter, filtrate is regulated pH value to 7.0 with 20% sodium hydroxide solution, adds to account for quality of liquid medicine 0.5% active carbon, keeps pH value 7.0, heat little boiling 15 minutes, cooling filters, and adds the injection water to 1000ml, canned, in 115 ℃ of sterilizations 30 minutes, cold preservation 48 hours filtered, packing promptly gets injection.
8. the described Chinese medicine composition of claim 1 to 5 is used for the treatment of application in the medicine of transmissible gastroenteritis of swine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148235.0A CN103211993B (en) | 2013-04-25 | 2013-04-25 | Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148235.0A CN103211993B (en) | 2013-04-25 | 2013-04-25 | Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103211993A true CN103211993A (en) | 2013-07-24 |
| CN103211993B CN103211993B (en) | 2014-08-27 |
Family
ID=48810379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310148235.0A Expired - Fee Related CN103211993B (en) | 2013-04-25 | 2013-04-25 | Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103211993B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905031A (en) * | 2015-05-20 | 2015-09-16 | 来英 | Pig gastroenteritis preventing and treating feed additive and production method thereof |
| CN106729589A (en) * | 2017-03-14 | 2017-05-31 | 韦煜辉 | Treat the Chinese medicine preparation and preparation method of transmissible gastroenteritis of swine |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411392A (en) * | 2008-11-18 | 2009-04-22 | 蒋勇 | Chinese herbal medicine additive agent for preventing and treating pig viral enteritis |
| CN101647996A (en) * | 2009-08-28 | 2010-02-17 | 天津亿才得科技有限公司 | Chinese medicine composition for treating grice enteritidis and preparation method thereof |
| CN101985022A (en) * | 2010-07-02 | 2011-03-16 | 荣成市科学技术情报研究所 | Chinese medicinal composition for treating damp heat enteritis |
| CN102058860A (en) * | 2009-11-14 | 2011-05-18 | 张其权 | Traditional Chinese medicine for treating pig chronic superficial gastritis |
| CN102526525A (en) * | 2012-02-06 | 2012-07-04 | 青岛农业大学 | Traditional Chinese medicine composition for treating transmissible gastroenteritis of swine |
| CN102698123A (en) * | 2012-01-05 | 2012-10-03 | 刘霞 | Traditional Chinese medicinal composition for treating transmissible gastroenteritis of swine, feed, preparation method and application thereof |
| CN102754734A (en) * | 2012-05-28 | 2012-10-31 | 鲁东大学 | Chinese herbal livestock feed additive and application thereof |
| CN103007160A (en) * | 2013-01-16 | 2013-04-03 | 青岛农业大学 | Pharmaceutical composition for treating pig infective gastrointestinal inflammation |
| CN103041307A (en) * | 2012-12-30 | 2013-04-17 | 青岛恒波仪器有限公司 | Traditional Chinese medicine composition for curing chronic ulcerative colitis and preparation method thereof |
-
2013
- 2013-04-25 CN CN201310148235.0A patent/CN103211993B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411392A (en) * | 2008-11-18 | 2009-04-22 | 蒋勇 | Chinese herbal medicine additive agent for preventing and treating pig viral enteritis |
| CN101647996A (en) * | 2009-08-28 | 2010-02-17 | 天津亿才得科技有限公司 | Chinese medicine composition for treating grice enteritidis and preparation method thereof |
| CN102058860A (en) * | 2009-11-14 | 2011-05-18 | 张其权 | Traditional Chinese medicine for treating pig chronic superficial gastritis |
| CN101985022A (en) * | 2010-07-02 | 2011-03-16 | 荣成市科学技术情报研究所 | Chinese medicinal composition for treating damp heat enteritis |
| CN102698123A (en) * | 2012-01-05 | 2012-10-03 | 刘霞 | Traditional Chinese medicinal composition for treating transmissible gastroenteritis of swine, feed, preparation method and application thereof |
| CN102526525A (en) * | 2012-02-06 | 2012-07-04 | 青岛农业大学 | Traditional Chinese medicine composition for treating transmissible gastroenteritis of swine |
| CN102754734A (en) * | 2012-05-28 | 2012-10-31 | 鲁东大学 | Chinese herbal livestock feed additive and application thereof |
| CN103041307A (en) * | 2012-12-30 | 2013-04-17 | 青岛恒波仪器有限公司 | Traditional Chinese medicine composition for curing chronic ulcerative colitis and preparation method thereof |
| CN103007160A (en) * | 2013-01-16 | 2013-04-03 | 青岛农业大学 | Pharmaceutical composition for treating pig infective gastrointestinal inflammation |
Non-Patent Citations (3)
| Title |
|---|
| 沈留红等: "腹腔灌注法配合中药对奶牛急性、慢性胃肠炎的疗效观察", 《黑龙江畜牧兽医》 * |
| 王恒恭: "中药试治疑似猪传染性胃肠炎", 《中兽医学杂志》 * |
| 陈义堂等: "猪流行性腹泻与传染性胃肠炎混感染的中药防治", 《山东畜牧兽医》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905031A (en) * | 2015-05-20 | 2015-09-16 | 来英 | Pig gastroenteritis preventing and treating feed additive and production method thereof |
| CN106729589A (en) * | 2017-03-14 | 2017-05-31 | 韦煜辉 | Treat the Chinese medicine preparation and preparation method of transmissible gastroenteritis of swine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103211993B (en) | 2014-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103202974A (en) | Traditional Chinese medicine composition for treating coccidiosis in chicken | |
| CN102085315B (en) | Traditional Chinese medicine composition for treating acute and chronic osteomyelitis | |
| CN103211993B (en) | Traditional Chinese medicine composition for treating porcine transmissible gastroenteritis | |
| CN102698158B (en) | Traditional Chinese medicine for preventing and treating infectious bronchitis, preparation method thereof and feed | |
| CN102309740A (en) | Traditional Chinese medicine for treating chronic enteritis | |
| CN104510940A (en) | Traditional Chinese medicinal formula for treating kidney stone | |
| CN104474104A (en) | Traditional Chinese medicine composition for preventing and treating infectious rhinitis of chicken and preparation method thereof | |
| CN104547048A (en) | Chinese herbal medicine compound preparation for preventing and treating infantile hand-foot-mouth disease and preparation method thereof | |
| CN102512595B (en) | Soup containing assorted traditional Chinese medicine for neck scrofula | |
| CN104547870B (en) | For treating Chinese medicine preparation of blue otopathy and its preparation method and application | |
| CN106039074A (en) | Tablets for treating canine parvovirus | |
| CN105250548A (en) | Treatment medicine for severe fever with thrombocytopenia syndrome | |
| CN104922410A (en) | TCM (traditional Chinese medicine) preparation for treating porcine circovirus disease | |
| CN104147295A (en) | Traditional Chinese medicine agentia for treating diarrhea | |
| CN104225569A (en) | Chinese medicinal composition for treating chronic enteritis | |
| CN101716307A (en) | Traditional Chinese medicine for treating AIDS | |
| CN105708933A (en) | Traditional Chinese medicine composition for preventing and treating hepatitis and preparation method thereof | |
| CN101085255A (en) | Traditional Chinese medicine preparation for treating acute nephritis | |
| CN105663657A (en) | Decoction medicine capable of effectively treating fire excess from yin deficiency and preparation method thereof | |
| CN104524321A (en) | Traditional Chinese medicinal preparation for treating epidemic hemorrhagic fever and acute renal failure | |
| CN105106848A (en) | Traditional Chinese medicine composition for preventing and treating hepatitis B | |
| CN104873857A (en) | Traditional Chinese medicine for treating alimentary infection | |
| CN102166295B (en) | Traditional Chinese medicine composition for treating carcinosis | |
| CN104435503A (en) | Traditional Chinese medicine anesthetic special for pre-operation and preparation method | |
| CN105616516A (en) | Epidemic cerebrospinal meningitis treating injection and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 Termination date: 20210425 |