CN103202392B - Application of NCG (N-CarbamylGlutamate) and VC (Vitamin C) in preparing heat stress-resisting feed additives or feeds - Google Patents
Application of NCG (N-CarbamylGlutamate) and VC (Vitamin C) in preparing heat stress-resisting feed additives or feeds Download PDFInfo
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Abstract
The invention provides application of NCG (N-CarbamylGlutamate) and VC (Vitamin C) in preparing heat stress-resisting feed additives or feeds. According to the invention, when the NCG and the VC are combined, the heat stress resisting ability of livestock (particularly sows) can be remarkably increased, so that the NCG and the VC produce a synergistic effect, can be widely popularized and applied in the field of animal husbandry and have good market effect and social effect.
Description
Technical field
The present invention relates to livestock culturing field, be specifically related to NCG and VC in the application of preparing in anti-heat stress feed additive or feed.
Background technology
Feed addictive refers to a small amount of or micro substance adding in Feed Manufacturing processing, use procedure, in feed consumption seldom but effect significantly.Feed addictive comprises the additive of nutritive additive, medicine class additive and some promotion digestion or metabolism, it,, to strengthening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.
N-carbamylglutamic acid (N-Carbamylglutamate, NCG), Chinese is N-carbamylglutamic acid.In the past, NCG mainly used the mankind, can significantly increase muscle on the one hand produce (containing) amount as nutrition fortifier, added in the product of the short muscle growth of body building; As medicine, be used for the treatment of some orphan disease of the mankind on the other hand: N-acetylglutamat as congenital in neonate (NAG) synzyme lacks the hyperammonemia and the rare genetic disease-maple syrup urine disease of neonate (Kalkan etc., 2009) that cause.
Recent research shows, NCG can promote arginic endogenous synthetic.Arginine is the free radical scavenger (Jiang Zhen etc., 2000) of body, and arginine and oxygen molecule oxygen generate nitric oxide (NO) through nitric oxide synthetase (NOS) catalysis.NO is a kind of novel cell informational molecule, has hemangiectasis, suppresses platelet aggregation, suppresses cell proliferation, suppresses the effects (Assumpcao etc., 2008) such as lipid peroxidation and advanced glycation end products formation.In animal body, appropriate NO can remove oxygen radical, effect ((Beligni etc., 2002 of performance anti-oxidation stress; Huang etc., 2009).Therefore, NCG, by promoting arginine endogenous synthetic, can improve multiparous animal nest litter size and birth weight, promotion young animal g and D.
Heat stress refers to the summation of nonspecific physiological reaction that any requirement that body in high ambient temperature proposes body thermal environment does.Non-specific physiological reaction main manifestations is: respiratory movement is accelerated, and carbon dioxide is got rid of to be increased, respiratory alkalosis; Heart failure, encephalemia, pulmonary edema, anoxic etc.; Vivo oxidation metabolism increases, and peroxide increases, film system injury; Metabolism is accelerated, hypoimmunity; The increase of urinating, ion is lost to be increased.Along with global warming, heat stress has become one of key factor of harm herding production.Animal including pig tends to produce heat stress, easily causes above-mentioned reaction and to cause appetite to decline even sick, and then impact growth.From different results of study, NCG adds in sow diets can improve production performance really, and determines that after deliberation the suitableeest addition of NCG in sow diets should be 0.5kg/ ton, and the optimum interpolation time is antenatal January-wean.But NCG is as a kind of conventional feed addictive, the anti-heat stress ability that it shows after adding in sow feed, and there is no at present bibliographical information with the effect that vitamin C is used in conjunction with.
Summary of the invention
The object of the invention is to overcome the defect of conventional feed anti-heat stress scarce capacity, provide NCG and vitamin C in the application of preparing in anti-heat stress feed additive and feed.
The present invention studies discovery, although use separately NCG and vitamin C to have to a certain extent the effect of anti-heat stress, effect degree is limited, be difficult to further raising, but, both are used in conjunction with to it and significantly improve in the effect aspect anti-heat stress, show that both have synergy.Based on this, the invention provides NCG and vitamin C in the application of preparing in anti-heat stress feed additive and feed.
Above-mentioned application, the preferred weight ratio of NCG and vitamin C is 1～2:1～2, more preferably 1:1.Content in feed is preferably 0.05～0.15%(w/w), be preferably 0.1%(w/w).
Above-mentioned feed and feed addictive, for improving the anti-heat stress ability of domestic animal, include but not limited to pig, especially say sow.
Above-mentioned feed addictive can directly make an addition in the daily ration of domestic animal, also can add with other feed addictives simultaneously, but should be noted that the combination matching of various compositions in the time adding.
Above-mentioned feed addictive should, to the time period interpolation between cub wean before production, can improve income and input ratio so to greatest extent.For example, sow should be first 1 month of production to adding feed addictive of the present invention between weaning period.
N-carbamylglutamic acid in the present invention is N-acetylglutamat (being NAG) analog, can as NAG, play a role in animal body and participate in body urea cycle, and long half time, metabolic stability, has biological function widely.Research shows, NCG can be used as metabolic activation agent and participates in dihydropyrrol-5-carboxylic acid synzyme (P5CS) and carbamyl phosphate synthetase I(CPS-I) activation, promote glutamine or proline synthesis citrulling, and then the synthetic (Wu etc. of promotion arginine (Arg), 2004), be also therefore referred to as activator in arginine.As NAG analog, NCG makes body arginine maintain higher physiological level, at the material that can be converted into important biomolecule activity after a series of metabolic processes, is mainly nitric oxide (NO) and polyamines (comprising putrescine, spermidine and spermine).By known above, arginine anti-oxidative stress in vivo is mainly realized by NO approach.
Vitamin C in the present invention is as water-soluble free radical scavenger, the reaction participating in is more than fat-soluble free radical scavenger vitamin E, the mechanism that visible vitamin C is removed free radical is also abundant than vitamin E, and vitamin C, except the function of free radical is removed in direct performance, can also assist vitamin E and glutathione to remove free radical.In swine rearing standard (NY/T65-2004) to ascorbic content in sow feed without explicitly calling for.In recent years since, vitamin C is improving reproductive capacity, the anti-heat stress ability of pig, prevents and treats the aspects such as infectious disease, poisoning and metabolic disease and finds broad application, and obtained significant effect (good fortune is established power, 2012).
The present invention makes an addition to NCG and vitamin C in sow feed and can reduce sow respiratory rate and body temperature under hot conditions, the content of MDA and cortisol in reduction blood, increase the content of immunoglobulin G and heat shock protein 70 in blood, especially show the content that has extremely significantly reduced MDA and cortisol in blood, reduce the generation of interior free yl, reduced the heat stress that high temperature produces sow.And the present invention's research shows, only use separately the anti-heat stress ability of NCG or vitamin C raising domestic animal very limited, but after both are combined, its anti-heat stress ability significantly improves, and shows that NCG and vitamin C have produced synergy.
Detailed description of the invention
Following examples are used for further illustrating the present invention, but should not be construed as limitation of the present invention.Do not deviating under the prerequisite of the present invention's spirit and essence, modification made for the present invention or replacement, all belong to category of the present invention.
Embodiment 1
1. material
1.1 additive N-carbamylglutamic acids (purity >=97%), vitamin C (purity >=99%)
1.2 animal genealogy of law large white sows (pig farm, large-scale kind of Beijing)
2. method and result
2.1 experimental design
Sow is tested 7～August in 2012 and carries out, and duration of test temperature on average is 25.8 degree, and daily maximum temperature average out to 30.2 is spent, and wherein daily maximum temperature is 47 days higher than the number of days of 30 DEG C; Medial humidity is 58.5%.
In sow feeding experiment, choose 120 of age, the subuniform genealogy of law large white sows of tire, be divided at random 6 groups, 20 every group.Between antenatal January-weaning period, accept at random the one processing in table 1 for every group, every group of additive addition using is in table 1.Use at additive start to record daily feed intake after 3 days, at participate in the experiment respiratory rate and the body temperature of pig of 14 mensuration every day, after childbirth, record every group of litter size, product young number alive, nascent litter weight, record wean young number alive, weight of weaning litter after 25 age in days wean, calculate respectively average respiratory rate, body temperature, nascent average individual weight and Weight each, gather vena cava anterior blood early morning on the same day at Farrowing, after separation of serum, measure MDA (MDA), immunoglobulin G (IgG), cortisol (Cor), heat shock protein 70 (HSP70) content.
Table 1 experimental design
2.2 result of the test
Between antenatal January-weaning period, add different additive sow respiratory rate, body temperature and feed intake have all been produced to impact (table 2).From table 2, in the antenatal January-feed for weaned of sow, add NCG, vitamin C or NCG and ascorbic composition can reduce sow respiratory rate and body temperature, improve the feed intake of sow under heat stress weather condition, especially obvious with C group.
The impact of table 2 different additive on sow respiratory rate, body temperature and feed intake
Note: the different lowercase alphabets of same row show significant difference (p<0.05).
Between antenatal January-weaning period, add different additive sow production performance has all been produced to impact (table 3).From table 3, between the antenatal January-weaning period of sow, in feed, add NCG, vitamin C or NCG and its production performance of ascorbic composition is all better than control group, wherein obvious with C group effect, while being mainly manifested in wean, can obtain maximum wean young number alive and individual counterpoise.
The impact of table 3 different additive on sow production performance
Note: the different lowercase alphabets of same row show significant difference (p<0.05), the different capitalizations of same row represent extremely significantly (p<0.01) of difference.
Between antenatal January-weaning period, add different additive sow anti-stress ability has all been produced to impact (table 4).From table 4, between the antenatal January-weaning period of sow, in feed, add NCG, vitamin C or NCG and its anti-stress ability of ascorbic composition makes moderate progress compared with control group, wherein obvious with C group effect, be mainly manifested in the content that reduces MDA and cortisol in blood, increase the content of immunoglobulin G and heat shock protein 70 in blood, especially show the content that has extremely significantly reduced MDA and cortisol in blood, reduce the generation of interior free yl, reduced the heat stress that high temperature produces sow.
The impact of table 4 different additive on sow anti-stress ability
Note: the different lowercase alphabets of same row show significant difference (p<0.05), the different capitalizations of same row represent extremely significantly (p<0.01) of difference.
Claims (3)
1.NCG and vitamin C are in the application of preparing in anti-heat stress feed additive or feed, and described NCG and ascorbic weight ratio are 1:1.
2. application according to claim 1, is characterized in that, described NCG and the vitamin C content in feed is 0.05～0.15% (w/w).
3. application according to claim 2, is characterized in that, described NCG and the vitamin C content in feed is 0.1% (w/w).
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| CN103518981A (en) * | 2013-08-27 | 2014-01-22 | 镇江天和生物技术有限公司 | Feed premix for preventing and treating heat stress of sows |
| CN104323034B (en) * | 2014-11-12 | 2017-12-22 | 江苏农牧科技职业学院 | A kind of feed that can improve broiler growth performance and its production and use |
| CN104431596B (en) * | 2014-12-18 | 2017-10-20 | 宁夏大学 | Improve the daily ration of drylot feeding sheep and goat carcass quality |
| CN104824377B (en) * | 2015-05-27 | 2017-12-26 | 北京市农林科学院 | One broad sow anti-heat stress promotees breeding compound additive |
| CN106721067A (en) * | 2016-12-15 | 2017-05-31 | 宁夏大学 | A kind of anti-stress feed addictive and its application process |
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| CA815054A (en) * | 1969-06-10 | Spa-Societa Prodotti Antibiotici S.P.A. | Salts of n-carbamyl-glutamic acid and of n-carbamyl-aspartic acid | |
| CN101953430B (en) * | 2010-07-15 | 2013-05-22 | 北京龙科方舟生物工程技术有限公司 | Feed, preparation method and use thereof in improvement in reproductive performance of sows |
| CN102106472B (en) * | 2010-12-07 | 2013-04-24 | 北京龙科方舟生物工程技术有限公司 | Complex additive for boars and preparation method and using method thereof |
| CN102423006A (en) * | 2011-11-15 | 2012-04-25 | 辽宁禾丰牧业股份有限公司 | Breastfeeding sow compound feed |
| CN102630638B (en) * | 2012-05-10 | 2014-01-15 | 广东温氏食品集团股份有限公司 | Method for improving the reproductive performance of sows |
| CN103125747B (en) * | 2013-03-22 | 2015-04-29 | 北京市农林科学院 | Feed additive and application thereof |
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Inventor after: Zeng Shaopeng Inventor after: Liu Yan Inventor after: Bai Jiahua Inventor after: Xu Xiaoling Inventor after: Guo Yong Inventor after: Huang Zheng Inventor before: Feng Tao Inventor before: Liu Yan Inventor before: Bai Jiahua Inventor before: Xu Xiaoling Inventor before: Guo Yong Inventor before: Huang Zheng |
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Effective date of registration: 20160310 Address after: 528100, No. 48-6, Datang garden, Sanshui Industrial Zone, Foshan, Guangdong Patentee after: FOSHAN BOEN BIOTECHNOLOGY CO., LTD. Address before: 100097 Beijing, Haidian District Shuguang Garden Road, No. 9 Patentee before: Beijing Academy of Agriculture and Forestry Sciences |