CN104286542A - Anti-stress regulator used for promoting growth and improving metabolism of pigs and preparation method thereof - Google Patents
Anti-stress regulator used for promoting growth and improving metabolism of pigs and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator is prepared from the following ingredients by weight: retinyl acetate, vitamin D3, d1-alpha-tocopheryl acetate, menadione, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, L-ascorbic acid, d-biotin, nicotinamide, pantothenic acid, folic acid, methionine, lysine, xylooligosaccharide, citric acid, sodium glutamate and glucose as balance. The invention also relates to a preparation method of the anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator prepared by the method can stimulate the self-metabolism regulation, can improve the feed utilization rate, can improve the food consumption of the growing-finishing pigs and the reproductive performance of the sows, can improve the anti-stress ability, can reduce the death rate and the diarrhea occurrence rate of piglets, has a remarkable growth promotion effect, can improve the growth speed of the weaned pigs, the growing-finishing pigs and the suckling pigs, and has remarkable economic benefit.
Description
Technical field
The invention belongs to feed pre-mixing material technical field, be specifically related to boar anti-stress growth promoting and improve metabolic conditioning agent, the invention still further relates to the preparation method that boar anti-stress growth promoting improves metabolic conditioning agent.
Background technology
Metabolism refers to that the nutriment that animal absorbs from the external world is converted into substance in vivo, simultaneously substance in vivo is converted into energy etc., and by process that useless material excretes.By metabolism, animal heat maintenance, contraction of muscle, glandular secretion and the vital movement such as growth, breeding could be realized.
The metabolism of pig is the same with other animals, comprise protein metabolism, glycometabolism, fat metabolism and water and minerals metabolism etc., the nutriment such as protein, fat and sugar class that pig is taken in, enzyme is with under the effect of other factors in vivo, first be decomposed into the simplest form, could absorbed by body and be converted into substance in vivo, and then carry out a series of metabolism, produce power utilizes for body simultaneously, and is excreted by useless even harmful metabolite.
Premix is the abbreviation of additive premix, it is by one or more microcomponents (comprising the additives such as various trace quantity mineral substance element, various vitamin, synthesizing amino acid, some drugs) and diluent or carrier proportioning on request, the osculant compound feed products made after Homogeneous phase mixing.Premix is a kind of important component of perfect compound feed.
At present, the additive used in feedstuff industry is still based on chemical synthesis, mainly comprise antibiotic, hormone, chemical synthetic drug etc., these additives improve metabolic method at pig anti-stress growth promoting and serve good effect, serve positive impetus to the development of feed industry and animal husbandry.But, along with increasing of clinical practice, the drawback of chemical synthesis additive also day by day manifests, as traditional intensive culture mode by adding tranquillizer chlorpromazine, the medicine such as stable carrys out retardation motion heat production to resist heat stress, but meeting remote-effects human health, China prohibits the use; While adding the growth of clenobuterol hydrochloride promotion pig, toxicity is transferred to mankind itself; Antibiotic use often causes the appearance of the endurance strain that toxicity is larger, the simultaneously generation of bacterial drug resistance and transfer, bacterium can be made again to produce drug resistance to people with antibiotic, cause difficulty to the treatment of infectious diseases clinically, European Union expressly forbids adding in pig feed for somatotrophic feeding antibiotic.
Summary of the invention
The object of this invention is to provide boar anti-stress growth promoting and improve metabolic conditioning agent, solve the body load existed in prior art large, can not fundamentally excite animal self active anti-stress growth promoting to improve metabolic problem.
Another object of the present invention is to provide the preparation method that a kind of boar anti-stress growth promoting improves metabolic conditioning agent.
First technical scheme of the present invention is, boar anti-stress growth promoting improves metabolic conditioning agent, with conditioning agent total amount, comprises following component: retinyl acetate 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters 2.5 × 10
4~ 3.8 × 10
4mg/kg, menadione 4.0 × 10
3~ 6.0 × 10
3mg/kg, thiamine hydrochloride 2.0 × 10
3~ 3.0 × 10
3mg/kg, riboflavin 4.5 × 10
3~ 6.5 × 10
3mg/kg, puridoxine hydrochloride 1.5 × 10
3~ 2.6 × 10
3mg/kg, cyanocobalamin 20 ~ 30mg/kg, L-AA 1.7 × 10
5~ 2.5 × 10
5mg/kg, D-biotin 175 ~ 264mg/kg, niacinamide 2.0 × 10
4~ 3.0 × 10
4mg/kg, pantothenic acid 0.85 × 10
4~ 1.32 × 10
4mg/kg, folic acid 0.80 × 10
3~ 1.2 × 10
3mg/kg, methionine 24 ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.40 × 10
4~ 3.60 × 10
4mg/kg, citric acid 2.00 × 10
5~ 3.00 × 10
5mg/kg, sodium glutamate 4.80 × 10
4~ 7.20 × 10
4mg/kg, supplies weight by glucose.
Second technical scheme of the present invention is, boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, and the concrete steps of described method are as follows:
Step 1, take various raw material by formula, with conditioning agent total amount, comprise following component: retinyl acetate 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters 2.5 × 10
4~ 3.8 × 10
4mg/kg, menadione 4.0 × 10
3~ 6.0 × 10
3mg/kg, thiamine hydrochloride 2.0 × 10
3~ 3.0 × 10
3mg/kg, riboflavin 4.5 × 10
3~ 6.5 × 10
3mg/kg, puridoxine hydrochloride 1.5 × 10
3~ 2.6 × 10
3mg/kg, cyanocobalamin 20 ~ 30mg/kg, L-AA 1.7 × 10
5~ 2.5 × 10
5mg/kg, D-biotin 175 ~ 264mg/kg, niacinamide 2.0 × 10
4~ 3.0 × 10
4mg/kg, pantothenic acid 0.85 × 10
4~ 1.32 × 10
4mg/kg, folic acid 0.80 × 10
3~ 1.2 × 10
3mg/kg, methionine 24 ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.40 × 10
4~ 3.60 × 10
4mg/kg, citric acid 2.00 × 10
5~ 3.00 × 10
5mg/kg, sodium glutamate 4.80 × 10
4~ 7.20 × 10
4mg/kg, supplies weight by glucose;
Step 2, by vitamin D
3, cyanocobalamin, D-biotin mix, and obtains once mixture;
Step 3, once mixture, retinyl acetate, thiamine hydrochloride, puridoxine hydrochloride, folic acid are carried out second time mixing, obtain secondary mixture;
Step 4, by secondary mixture, menadione, riboflavin, pantothenic acid, niacinamide, VE acetic acid esters, xylo-oligosaccharide, carry out third time mixing, obtain three mixtures;
Step 5, three mixtures, sodium glutamate, citric acid, L-AA, methionine, lysines are carried out the 4th mixing, obtain four mixtures;
Step 6, add in four mixtures rated weight glucose carry out the 5th time mixing, namely obtain finished product.
Feature of the present invention is also,
Further, with conditioning agent total amount, comprise following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.00 × 10
4mg/kg, citric acid 2.50 × 10
5mg/kg, sodium glutamate 6.00 × 10
4mg/kg, supplies weight by glucose.
Further, with conditioning agent total amount, comprise following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 6.00 × 10
4mg/kg, citric acid 3.0 × 10
5mg/kg, sodium glutamate 5.0 × 10
4mg/kg, supplies weight by glucose.
Further, with conditioning agent total amount, comprise following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.60 × 10
4mg/kg, citric acid 3.00 × 10
5mg/kg, sodium glutamate 7.20 × 10
4mg/kg, residuals weight supplied by glucose.
The invention has the beneficial effects as follows: the science by vitamin, amino acid, citric acid, xylo-oligosaccharide, sodium glutamate etc. in the present invention is composite, provide one and there is resisting stress, growth promotion, improvement metabolism, and not containing hormone, antibiotic safe, green, non-harmful pig conditioning agent; The present invention, by regulating vitamin, amino acid whose screening and consumption, makes its structure more reasonable, is conducive to absorbing in pig body; The Special attention will be given to application of sodium glutamate, citric acid and xylo-oligosaccharide in the present invention, while improving the palatability of conditioning agent, the more important thing is and regulate pig body metabolism, realized the effect of anti-stress growth promoting by the change of body self, impel body to change into present active adjustment from original passive adjustment.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
The invention provides boar anti-stress growth promoting and improve metabolic conditioning agent, with conditioning agent total amount, comprise following component: retinyl acetate (vitamin A) 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters (vitamin E) 2.8 ~ 3.5 × 10
4mg/kg, menadione (MSB) (vitamin k
3) 4.2 ~ 6.2 × 10
3mg/kg, thiamine hydrochloride (Cobastab
1) 2.0 ~ 3.0 × 10
3mg/kg, riboflavin (Cobastab
2) 4.40 ~ 6.5 × 10
3mg/kg, puridoxine hydrochloride (Cobastab
6) 1.8 ~ 2.6 × 10
3mg/kg, cyanocobalamin (Cobastab
12) 20 ~ 30mg/kg, L-AA (vitamin C) 1.7 ~ 2.5 × 10
5mg/kg, D-biotin 180 ~ 260mg/kg, niacinamide 2.0 ~ 3.0 × 10
4mg/kg, pantothenic acid 1.0 ~ 1.3 × 10
4mg/kg, folic acid 0.8 ~ 1.2 × 10
3mg/kg, methionine 24g/kg ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.4 ~ 3.6 × 10
4mg/kg, citric acid 2.0 ~ 3.0 × 10
5mg/kg, sodium glutamate 5.0 ~ 7.0 × 10
4mg/kg.
Sodium glutamate can be converted into glutamic acid under acid condition under one's belt, and being the coded amino acid in protein synthesis, is the most important amino acid of intestinal mucosa oxidation substrates, in gastrointestinal tract mucous preferential utilization, has the effect regulating Nutrition and Metabolism; The glutamic acid that enteron aisle is partial to utilize the glutamic acid in enteron aisle instead of glutamine metabolism to produce is with synthesizing glutathion, and glutamic acid is also the required precursor synthesizing glutamine needed for intestinal regeneration and other quick value-added cells in body, illustrates in daily ration that adding sodium glutamate can save branched-chain amino acid.Sodium glutamate growth promotion improves metabolic aspect and shows as: fat percentage and the leg stern ratio that can increase pig; Significantly improve yellowish pink and the marble grain scoring of biceps muscle of thigh; Promote the Fatty synthesis metabolism of biceps muscle of thigh, increase IMF content; Improve the ratio of unrighted acid and saturated fatty acid, increase the content of C8:1, C10:1 and trans C18:1 in biceps muscle of thigh, reduce the content of C15:1, C16:1, C18:1, C20:1, C22:1, C22:2, C24:0 and C24:1, thus regulate aliphatic acid metabolism in vivo.
Citric acid is one of important substance forming respiratory chain in mitochondria, is the important medium in CoA synthetic fatty acid process, can regulates tricarboxylic acid cycle Mitochondria adenylate cyclase activity; The omnipotent link forming bio-energy aerobic processes in tricarboxylic acid cycle process: citric acid passes through repeatedly decarboxylation and can generate, and provides the energy of 30 ATP; Citric acid in vivo metabolic conversion is CO
2and HCO
3 -, maintain the acid-base balance of blood, body fluid, ensure the metastable environment of body.The pH level that citric acid can reduce gastric content is added in diet; Improve small intestine contents trypsase and amylase activity; Improve beneficial microorganism be suitable for living environment or directly suppress, kill harmful microorganism; Promote the fermentation of lactic acid bacteria simultaneously, enhance metabolism, strengthen energy and amino acid whose utilization; The gastric emptying speed that slows down and food, in enteron aisle internal migration speed, are conducive to digesting and assimilating of food Middle nutrition material; While reducing gastrointestinal content pH, form easy absorbed complex compound with some mineral elements, be conducive to being absorbed by body, operate and utilizing; There is sealing process to feed metal ion, avoid catalytic oxidation, play the protectant effect of feed; Under stress situation, can be used for the urgent synthesis of ATP, thus improve anti-stress ability, reduce engine soma decomposes.
Xylo-oligosaccharide is that each D-wood sugar of 2-7 combines the straight chain sugar formed through β-Isosorbide-5-Nitrae glycosidic bond.Xylo-oligosaccharide optionally can promote beneficial bacterium rapidly, suppresses the propagation of harmful bacteria, thus improves intestinal microenvironment, reduces harmful bacteria and produce noxious material and cause damage to body; Utilized by bifidobacterium fermentation, synthesis and multivitamin absorption such as promotion nicotinic acid, folic acid etc.; Be that SCFA makes intestinal pH decline by bifidobacterium fermentation, be combined with trace element, increase solubility, and stimulate colon theca cell, improve enteron aisle to the absorption of mineral matter.Therefore, can organism metabolism be improved in animal productiong, promote growth of animals or poultry, improve the production performance of livestock and poultry, improve animal health level, prevent disease, raising animal immunizing power.
The present invention, on the basis of vitamin, amino acid material science compatibility and basic effect, adds xylo-oligosaccharide, citric acid and sodium glutamate innovation composition.While improving the palatability of conditioning agent, the more important thing is and regulate pig body metabolism, realized the effect of anti-stress growth promoting by the change of body self, impel body to change into present active adjustment from original passive adjustment.
Throw composition labelled amount be all up to the standards in 80%-120% category.Cut lower than 80% uncertain therapeutic efficacy, feeding intake higher than 120% one is that to there is waste two be according to dosage use portioned product easily to occur poisoning or other bad reactions.
The present invention also provides boar anti-stress growth promoting to improve the preparation method of metabolic conditioning agent, comprises the following steps:
Step 1, with conditioning agent total amount, take following component by formula: retinyl acetate (vitamin A) 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters (vitamin E) 2.8 ~ 3.5 × 10
4mg/kg, menadione (MSB) (vitamin k
3) 4.2 ~ 6.2 × 10
3mg/kg, thiamine hydrochloride (Cobastab
1) 2.0 ~ 3.0 × 10
3mg/kg, riboflavin (Cobastab
2) 4.40 ~ 6.5 × 10
3mg/kg, puridoxine hydrochloride (Cobastab
6) 1.8 ~ 2.6 × 10
3mg/kg, cyanocobalamin (Cobastab
12) 20 ~ 30mg/kg, L-AA (vitamin C) 1.7 ~ 2.5 × 10
5mg/kg, D-biotin 180 ~ 260mg/kg, niacinamide 2.0 ~ 3.0 × 10
4mg/kg, pantothenic acid 1.0 ~ 1.3 × 10
4mg/kg, folic acid 0.8 ~ 1.2 × 10
3mg/kg, methionine 24g/kg ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.4 ~ 3.6 × 10
4mg/kg, citric acid 2.0 ~ 3.0 × 10
5mg/kg, sodium glutamate 5.0 ~ 7.0 × 10
4mg/kg;
Step 2, by vitamin D
3, cyanocobalamin, D-biotin mix, and obtains once mixture;
Step 3, once mixture, retinyl acetate, thiamine hydrochloride, puridoxine hydrochloride, folic acid are carried out second time mixing, obtain secondary mixture;
Step 4, by secondary mixture, menadione, riboflavin, pantothenic acid, niacinamide, VE acetic acid esters, xylo-oligosaccharide, carry out third time mixing, obtain three mixtures;
Step 5, three mixtures, sodium glutamate, citric acid, L-AA, methionine, lysines are carried out the 4th mixing, obtain four mixtures;
Step 6, add in four mixtures rated weight glucose carry out the 5th time mixing, namely obtain finished product.
Vitamin D is adopted in step 2
3, cyanocobalamin, the low dose of composition mixing of D-biotin, and then step by step add heavy dose of composition, this conditioning agent can be made mix more fully, regulating effect is better.
Embodiment 1
One boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, comprises the following steps:
(1) various raw material is taken by formula: retinyl acetate (vitamin A) 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters (vitamin E) 3.20 × 10
4mg/kg, menadione (MSB) (vitamin k
3) 5.20 × 10
3mg/kg, thiamine hydrochloride (Cobastab
1) 2.60 × 10
3mg/kg, riboflavin (Cobastab
2) 5.40 × 10
3mg/kg, puridoxine hydrochloride (Cobastab
6) 2.20 × 10
3mg/kg, cyanocobalamin (Cobastab
12) 25mg/kg, L-AA (vitamin C) 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.00 × 10
4mg/kg, citric acid 2.50 × 10
5mg/kg, sodium glutamate 6.00 × 10
4mg/kg, all the other are glucose;
(2) by vitamin D
3, cyanocobalamin, D-biotin mix, and obtains once mixture;
(3) once mixture, retinyl acetate, thiamine hydrochloride, puridoxine hydrochloride, folic acid are carried out second time mixing, obtain secondary mixture;
(4) by secondary mixture, menadione, riboflavin, pantothenic acid, niacinamide, VE acetic acid esters, xylo-oligosaccharide, carry out third time mixing, obtain three mixtures;
(5) three mixtures, sodium glutamate, citric acid, L-AA, methionine, lysines are carried out the 4th mixing, obtain four mixtures;
(6) in four mixtures, add rated weight glucose carry out the 5th mixing, namely obtain finished product.
Described in embodiment 1, pig anti-stress growth promoting improves metabolic conditioning agent, is applicable to weanling pig, and adding proportion is 0.1%, can effectively improve weanling pig anti-stress ability, has obvious growth promotion to improve metabolic effect.
The impact of metabolic conditioning agent on piglet growth performance is improved with anti-stress growth promoting below in conjunction with concrete description of test embodiment 1 pig.
1. materials and methods
1.1 for examination pig and grouping
Two process are established in this test, each process three repetition, according to the principle of " parity is consistent, body weight is close, male and female half and half ", by " DLY " tri-crossbreeding 60 random packet of about 9kg.7 days preliminary trial periods, 40 days experimental periods.With reference to NRC (1998) " nutritional need of pig ", the basal diet of design experiment pig is as table 1, and on the basis of control group basal diet, this adds the premix described in embodiment 1 to test group daily ration.
1.2 basal diets and feeding and management basal diet composition and trophic level in table 1.
The composition of table 1 basal diet
| Corn/% | 64 |
| Expanded soybean/% | 8 |
| Dregs of beans/% | 22 |
| Fish meal/% | 2 |
| Premix/% | 4 |
| Add up to | 100 |
Every day is recorded the situations such as test pig appetite, spirit, ight soil, morbidity in 1.3 performance tests, and the feed intake of every day often organized in record.The first day started respectively at formal test, off-test day, morning empty stomach is carried out to all test pig and weigh.Calculate daily gain, daily ingestion amount and feed-weight ratio.
1.4 data processing test datas represent with average ± standard deviation, carry out one-way analysis of variance.All data SPSS statistics software carries out treatment and analyses.
2. during test results test, control group and test group piglet search for food, ight soil etc. is all normal.Compared with control group, test group piglet grazing speed is obviously very fast, and during off-test, body weight is obviously heavier, and hair color is brighter, and meanwhile, in process of the test, Incidence of Diarrhea is lower.Whole duration of test, control group and test group feed intake are without obvious difference, but from 4th week, test group weight gain of piglets is obviously very fast, and difference is extremely significantly (p ﹤ 0.01) compared with control group; Duration of test, control group piglet daily gain is 0.317kg, and test group is 0.388kg, and gaining effect is obvious.In whole process of the test, control group feedstuff-meat ratio all pole is significantly higher than test group, and illustrate that test group pig starter feed utilization rate is higher, gaining effect is obvious.In table 2 and table 3.
In sum, anti-stress growth promoting described in embodiment 1 improves the speed of growth that modulators of metabolism can significantly improve weanling pig, improves efficiency of feed utilization, reduces grice diarrhoea incidence, has the effect significantly improving weaned piglets.
Table 2 test group compares with control group production performance
| Index/kg | The last week | The first two week | First three week | Front surrounding | Quan Qi | |
| Daily gain | Control group | 0.214±0.025 a | 0.249±0.016 a | 0.280±0.017 a | 0.299±0.012 a | 0.317±0.031 a |
| Daily gain | Test group | 0.222±0.134 a | 0.282±0.011 a | 0.322±0.012 a | 0.364±0.013 B | 0.388±0.272 B |
| Feed intake | Control group | 0.422±0.085 a | 0.503±0.060 a | 0.606±0.052 a | 0.666±0.039 a | 0.732±0.050 a |
| Feed intake | Test group | 0.378±0.056 a | 0.494±0.047 a | 0.580±0.027 a | 0.671±0.033 a | 0.740±0.046 a |
| Feedstuff-meat ratio | Control group | 1.98±0.10 a | 2.03±0.12 a | 2.16±0.09 a | 2.23±0.08 a | 2.31±0.12 a |
| Feedstuff-meat ratio | Test group | 1.72±0.31 B | 1.75±0.14 B | 1.81±0.06 B | 1.86±0.04 B | 1.90±0.13 B |
Table 3 test group increases weight with control group and compares with diarrhea rate
| Index | Control group | Test group |
| First starting weight/kg | 8.09±0.16 a | 8.15±0.18 a |
| End weight/kg | 19.21±0.99 a | 21.75±0.90 B |
| Diarrhea rate/% | 6.67 | 3.33 |
Note: the data shoulder marker tape of different disposal has identical lowercase alphabet differential different not significantly (p ﹥ 0.05), show significant difference (p ﹤ 0.05) with different lowercase alphabets, represent that when lowercase is different with capitalization difference extremely significantly (p ﹤ 0.01).
Embodiment 2
One boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, comprises the following steps: (1) takes various raw material by formula: retinyl acetate (vitamin A) 6,200,000 IU/kg, vitamin D
36000000 IU/kg, VE acetic acid esters (vitamin E) 3.20 × 10
4mg/kg, menadione (MSB) (vitamin k
3) 5.20 × 10
3mg/kg, thiamine hydrochloride (Cobastab
1) 2.60 × 10
3mg/kg, riboflavin (Cobastab
2) 5.40 × 10
3mg/kg, puridoxine hydrochloride (Cobastab
6) 2.20 × 10
3mg/kg, cyanocobalamin (Cobastab
12) 25mg/kg, L-AA (vitamin C) 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 6.00 × 10
4mg/kg, citric acid 3.0 × 10
5mg/kg, sodium glutamate 5.0 × 10
4mg/kg, residuals weight supplied by glucose.All the other steps are with embodiment 1.
Described in embodiment 2, pig anti-stress growth promoting improves metabolic conditioning agent, is applicable to each growth phase of growing and fattening pigs, and adding proportion is 0.2%.
The impact of metabolic conditioning agent on pig growth performance is improved below in conjunction with description of test embodiment 2 anti-stress growth promoting.
1. materials and methods
1.1 for examination pig and grouping
According to the principle of " parity is consistent, body weight is close, male and female half and half ", select the growing-finishing pig 60 that age birthday, body weight etc. are basically identical, be divided into 2 groups at random.7 days preliminary trial periods, carry out playing overbit, epidemic prevention and expelling parasite.Weigh on an empty stomach at the end of preliminary trial period, record, enter the formal test phase, 90 days experimental periods.Feeding and management is undertaken by pig farm code.
1.2 feeding and management test pig are fed corn-soybean meal diet.With reference to NRC (1998) " nutritional need of pig ", the basal diet of design experiment pig is as table 4.On the basis of control group basal diet, this adds the premix described in embodiment 2 to test group daily ration.Growth period, feeding time was 43 days, and pig body weight is between about 25kg to about 55kg; Fattening period feeding time is 47 days, and pig body weight is between about 55kg to about 90kg.Duration of test, pig free choice feeding and freely drinking water, and weigh on an empty stomach respectively at the end of growth period and fattening period, sacrificed by exsanguination, samples and analyzes following index: dressing percentage, trunk composition, skin depth and the thickness of backfat, leg stern ratio, pH, yellowish pink, marble grain, cold cuts rate, percentage of water loss, dry, crude fat and the index such as thick protein, middle LCFA.
The composition of table 4 basal diet
| The 20-55kg stage | The 55-90kg stage | |
| Corn/% | 67.0 | 63.5 |
| Wheat bran/% | 7.3 | 13.7 |
| Dregs of beans/% | 19.4 | 18.0 |
| Fish meal/% | 3.0 | 2.0 |
| Stone flour/% | 0.7 | 0.8 |
| Calcium monohydrogen phosphate/% | 1.0 | 0.5 |
| Salt/% | 0.3 | 0.3 |
| Lysine/% | 0.3 | 0.2 |
| Premix/% | 1.0 | 1.0 |
1.3 data processing test datas represent with average ± standard deviation, carry out one-way analysis of variance.All data SPSS statistics software carries out treatment and analyses.
2. the control group average daily gain of result of the test grower pigs stage is 0.691kg, and test group is 0.764kg, increases by 10.56% than control group, obviously (p ﹤ 0.05), feedstuff-meat ratio is respectively 2.208 and 2.037 to gaining effect, and the two compares significant difference (p ﹤ 0.05); Growing and fattening pigs stage control group and test group average daily gain are respectively 0.604kg and 0.726kg, and test group improves 20.19% than control group, and gaining effect is obvious, and feedstuff-meat ratio is respectively 3.628 and 3.302, and the two compares significant difference.Contrast whole duration of test, control group Average weight increasing a day is 0.646kg, test group is 0.744kg, and comparatively control group improves 15.17%, has extremely significant effect of gain, average daily feed intake is respectively 1.895kg and 2.035kg, the two compares significant difference, and illustrate that described in embodiment 2, premix can significantly improve the feed intake of growing-finishing pig, the two feedstuff-meat ratio is respectively 2.941 and 2.740, test group comparatively control group reduces by 6.83%, significant difference.Experimental group is compared with control group, and carcass trait and composition there occurs obvious change, and conditioning agent adds leg stern ratio and fat percentage, the yellowish pink of biceps muscle of thigh and marble grain scoring all significantly increase, IMF content also has increase, and Fatty acid compositions improves, and the results are shown in Table 5-table 9.
In sum, anti-stress growth promoting described in embodiment 2 improves the speed of growth that modulators of metabolism can significantly improve growing-finishing pig, improves metabolism, improves feed intake and the price of deed, has obvious economic benefit.
Table 5 anti-stress growth promoting improves the impact of metabolic conditioning agent on store pig production performance
| Index | Control group | Test group |
| Beginning counterpoise/kg | 25.106±0.847 a | 24.984±1.183 a |
| End counterpoise/kg | 83.218±3.592 a | 91.964±3.376 B |
| Growth phase Average weight increasing a day/kg | 0.691±0.023 a | 0.764±0.046 b |
| Average daily feed intake/the kg of growth phase | 1.525±0.031 a | 1.553±0.047 a |
| Growth phase feedstuff-meat ratio | 2.208±0.080 a | 2.037±0.094 b |
| Fattening stage Average weight increasing a day/kg | 0.604±0.075 a | 0.726±0.048 b |
| Average daily feed intake/the kg of fattening stage | 2.177±0.140 a | 2.387±0.196 a |
| Fattening stage feedstuff-meat ratio | 3.628±0.246 a | 3.302±0.185 b |
| Test Average weight increasing a day/kg of full phase | 0.646±0.041 a | 0.744±0.045 B |
| Test average daily feed intake/kg of full phase | 1.895±0.051 a | 2.035±0.098 b |
| Test full phase feedstuff-meat ratio | 2.941±0.131 a | 2.740±0.162 b |
Note: the data shoulder marker tape of different disposal has identical lowercase alphabet differential different not significantly (p ﹥ 0.05), show significant difference (p ﹤ 0.05) with different lowercase alphabets, represent that when lowercase is different with capitalization difference extremely significantly (p ﹤ 0.01).
Table 6 conditioning agent is on the impact of growing and fattening quality of pig trunk
| Project | Control group | Experimental group |
| The thickness of backfat (cm) | 1.35±0.12 | 1.29±0.11 |
| Skin depth (cm) | 0.32±0.03 | 0.33±0.03 |
| Lean meat percentage (%) | 60.8±3.11 | 60.1±0.54 |
| Fat percentage (%) | 13.5±0.92 a | 16.7±0.37 b |
| Bone rate (%) | 17.2±0.34 | 15.6±0.55 |
| Skin rate (%) | 8.5±0.34 | 8.8±0.33 |
| Dressing percentage (%) | 68.5±0.74 a | 66.4±0.46 b |
| Leg stern ratio (%) | 29.5±0.76 a | 34.2±0.53 b |
Note: data representation format is mean+SD.Colleague's subscript difference represents significant difference (p ﹤ 0.05), and table 7-table 9 is same.
Table 7 conditioning agent is on the impact of growing and fattening pig flesh characters
| Project | Control group | Experimental group |
| Yellowish pink | 2.50±0.12 a | 3.79±0.15 b |
| Marble grain | 2.72±0.13 a | 3.33±0.23 b |
| Cold cuts rate (%) | 67.8±3.21 | 69.1±3.54 |
| Percentage of water loss (%) | 29.5±2.92 | 29.7±2.36 |
| Muscle pH (butchering rear 45min) | 6.2±0.34 | 6.2±0.15 |
| Muscle pH (butchering rear 24h) | 5.56±0.34 | 5.58±0.33 |
Table 8 conditioning agent is on the impact of growing-finishing pig muscle chemical composition
| Project (%) | Control group | Experimental group |
| Dry | 28.50±0.12 | 27.79±0.75 |
| Thick protein | 21.72±0.13 | 20.33±0.23 |
| Crude fat (IMF) | 3.08±0.21 a | 4.01±0.54 b |
The impact that table 9 conditioning agent forms growing-finishing pig intramuscular fatty acid
| Project | Control group | Experimental group |
| C8:1 | 0.03±0.002 a | 1.29±0.11 b |
| C10:1 | 0.09±0.003 a | 0.83±0.3 b |
| C14:1 | 0.89±0.11 | 0.71±0.54 |
| C16:0 | 33.5±3.92 | 21.7±3.7 |
| C15:1 | 0.06±0.03 a | 0.04±0.01 b |
| C16:1 | 0.75±0.14 a | 0.48±0.03 b |
| C18:1 | 28.5±2.74 a | 16.4±1.46 b |
| C18:1trans | 15.5±1.76 a | 20.2±1.53 b |
| C18:2 | 12.5±1.12 | 15.9±2.1 |
| C20:0 | 0.02±0.01 a | 0.01±0.0 b |
| C18:3 | 3.2±0.11 | 3.91±0.54 |
| C22:0 | 0.15±0.02 a | 0.05±0.01 b |
| C20:3 | 0.45±0.04 | 0.43±0.05 |
| C22:1 | 0.15±0.04 a | 0.05±0.01 b |
| C22:2 | 0.15±0.04 a | 0.07±0.01 b |
| C24:0 | 0.09±0.01 a | 0.03±0.00 b |
| C24:1 | 0.09±0.02 a | 0.05±0.01 b |
| C22:6 | 14.3±1.03 a | 13.3±0.03 |
| Saturated fatty acid | 24.8±3.11 | 21.1±0.54 |
| Unrighted acid | 75.5±6.92 | 78.7±5.37 |
| MUFA | 47.2±5.34 | 45.6±3.55 |
| PUFA | 30.5±2.34 | 33.8±4.33 |
Embodiment 3
One boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, comprises the following steps: (1) takes various raw material by formula: retinyl acetate (vitamin A) 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters (vitamin E) 3.20 × 10
4mg/kg, menadione (MSB) (vitamin k
3) 5.20 × 10
3mg/kg, thiamine hydrochloride (Cobastab
1) 2.60 × 10
3mg/kg, riboflavin (Cobastab
2) 5.40 × 10
3mg/kg, puridoxine hydrochloride (Cobastab
6) 2.20 × 10
3mg/kg, cyanocobalamin (Cobastab
12) 25mg/kg, L-AA (vitamin C) 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.60 × 10
4mg/kg, citric acid 3.00 × 10
5mg/kg, sodium glutamate 7.20 × 10
4mg/kg, residuals weight supplied by glucose.All the other steps are with embodiment 1.
Described in embodiment 3, pig anti-stress growth promoting improves metabolic conditioning agent, is applicable to herd boar, broad sow and in-pig, and adding proportion is 0.05%.
The impact of metabolic conditioning agent on pig growth performance is improved below in conjunction with description of test embodiment 3 anti-stress growth promoting.
1. materials and methods
1.1, for examination pig and a point group selection parity (2 ~ 3 tire), the sow 40 that grows up that body condition is close, are divided into 2 groups successively according to breeding order, often organize 20, identical Duroc boars remating.
1.2 feeding and management test pig are fed corn-soybean meal diet.With reference to NRC (1998) " nutritional need of pig ", the basal diet of design experiment pig is as table 10.On the basis of control group basal diet, this adds the premix described in embodiment 3 to test group sow diets.
The composition of table 10 basal diet
| Gestation daily ration | Lactation daily ration | |
| Corn/% | 58 | 59 |
| Wheat bran/% | 23 | 18 |
| Dregs of beans/% | 13 | 17 |
| Fish meal/% | 2 | 2 |
| Premix/% | 0.05 | 0.05 |
1.3 Testing index detect the product often organizing test sow young mean alive, weigth at birth, first repiece counterpoise, wean in 28 days young number alive and weight of weaning litter, and analyze the comprehensive index of reproduction of each group of sow.
1.4 data processing test datas represent with average ± standard deviation, carry out one-way analysis of variance.All data SPSS statistics software carries out analyzing and processing.
2. result of the test
Duration of test, all there is not disease in test sow, the situation such as diet, spirit is all normal, and the situations such as miscarriage do not occur.Control group and the average number born alive of test group are respectively 9.85 and 11.15, and the two compares significant difference (p ﹤ 0.05), and during to 28 age in days wean, young number of living is respectively 9.35 and 10.85, and the death rate is respectively 5.08% and 2.69%.Control group piglet weigth at birth is 13.68kg, and test group is 16.27kg, improves 18.93% than control group, and be 64.81kg to control group litter weight during 28 age in days wean, test group is 77.05kg, and the two is pole significant difference (p ﹤ 0.01).Test group and the control group sow condition of production are in table 11.
In sum, compared with common premix product, add premix described in embodiment 3 in in-pig daily ration and can significantly improve sow reproductive performance and promote suckling pig growth, reduce the piglet death rate, there is obvious economic benefit.
Table 11 control group and test group sow condition of production contrast table
| Index | Control group | Test group |
| Test sow/head | 20 | 20 |
| Produce young mean/head of living | 9.85±1.79 a | 11.15±1.66 b |
| Weigth at birth/kg | 13.68±2.08 a | 16.27±2.74 b |
| First repiece counterpoise/kg | 1.40±0.07 a | 1.49±0.09 b |
| Wean young number for 28 days | 9.35±2.03 a | 10.85±2.08 b |
| 28 days weight of weaning litters | 64.81±9.05 a | 77.05±8.81 b |
| The comprehensive index of reproduction of sow | 106.65 | 124.80 |
Note: represent that two groups of data compare significant difference (p ﹤ 0.05) with data line lowercase difference person, identical person represents that two groups of data compare difference not significantly (p ﹥ 0.05).
Note: the comprehensive index of reproduction SPI=2.67NBA+1.87N of sow
28+ 0.97W
28, in formula, NBA is number born alive, N
28, W
28for 28 days nests respectively wean young number and litter weight.
Embodiment 4
One boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, comprises the following steps: (1) takes various raw material by formula: with conditioning agent total amount, comprises following component: retinyl acetate 3,500,000 IU/kg, vitamin D
34600000 IU/kg, VE acetic acid esters 2.5 × 10
4mg/kg, menadione 6.0 × 10
3mg/kg, thiamine hydrochloride 2.0 × 10
3mg/kg, riboflavin 6.5 × 10
3mg/kg, puridoxine hydrochloride 1.5 × 10
3mg/kg, cyanocobalamin 30mg/kg, L-AA 1.7 × 10
5mg/kg, D-biotin 264mg/kg, niacinamide 2.0 × 10
4mg/kg, pantothenic acid 1.32 × 10
4mg/kg, folic acid 0.80 × 10
3mg/kg, methionine 36g/kg, lysine 8g/kg, xylo-oligosaccharide 3.60 × 10
4mg/kg, citric acid 2.00 × 10
5mg/kg, sodium glutamate 7.20 × 10
4mg/kg, supplies weight by glucose.All the other steps are with embodiment 1.
Embodiment 5
One boar anti-stress growth promoting improves the preparation method of metabolic conditioning agent, comprises the following steps: (1) takes various raw material by formula: with conditioning agent total amount, comprises following component: retinyl acetate 6,200,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.8 × 10
4mg/kg, menadione 4.0 × 10
3mg/kg, thiamine hydrochloride 3.0 × 10
3mg/kg, riboflavin 4.5 × 10
3, puridoxine hydrochloride 2.6 × 10
3mg/kg, cyanocobalamin 20mg/kg, L-AA 2.5 × 10
5mg/kg, D-biotin 175mg/kg, niacinamide 3.0 × 10
4mg/kg, pantothenic acid 0.85 × 10
4mg/kg, folic acid 1.2 × 10
3mg/kg, methionine 24g/kg, lysine 12g/kg, xylo-oligosaccharide 2.40 × 10
4mg/kg, citric acid 3.00 × 10
5mg/kg, sodium glutamate 4.80 × 10
4mg/kg, supplies weight by glucose.All the other steps are with embodiment 1.
Claims (5)
1. boar anti-stress growth promoting improves metabolic conditioning agent, it is characterized in that, with conditioning agent total amount, comprises following component: retinyl acetate 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters 2.5 × 10
4~ 3.8 × 10
4mg/kg, menadione 4.0 × 10
3~ 6.0 × 10
3mg/kg, thiamine hydrochloride 2.0 × 10
3~ 3.0 × 10
3mg/kg, riboflavin 4.5 × 10
3~ 6.5 × 10
3mg/kg, puridoxine hydrochloride 1.5 × 10
3~ 2.6 × 10
3mg/kg, cyanocobalamin 20 ~ 30mg/kg, L-AA 1.7 × 10
5~ 2.5 × 10
5mg/kg, D-biotin 175 ~ 264mg/kg, niacinamide 2.0 × 10
4~ 3.0 × 10
4mg/kg, pantothenic acid 0.85 × 10
4~ 1.32 × 10
4mg/kg, folic acid 0.80 × 10
3~ 1.2 × 10
3mg/kg, methionine 24 ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.40 × 10
4~ 3.60 × 10
4mg/kg, citric acid 2.00 × 10
5~ 3.00 × 10
5mg/kg, sodium glutamate 4.80 × 10
4~ 7.20 × 10
4mg/kg, supplies weight by glucose.
2. pig anti-stress growth promoting as claimed in claim 1 improves metabolic conditioning agent, it is characterized in that: further, with conditioning agent total amount, comprises following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.00 × 10
4mg/kg, citric acid 2.50 × 10
5mg/kg, sodium glutamate 6.00 × 10
4mg/kg, supplies weight by glucose.
3. pig anti-stress growth promoting as claimed in claim 1 improves metabolic conditioning agent, it is characterized in that: further, with conditioning agent total amount, comprises following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 6.00 × 10
4mg/kg, citric acid 3.0 × 10
5mg/kg, sodium glutamate 5.0 × 10
4mg/kg, supplies weight by glucose.
4. pig anti-stress growth promoting as claimed in claim 1 improves metabolic conditioning agent, it is characterized in that: further, with conditioning agent total amount, comprises following component: retinyl acetate 3,500,000 IU/kg, vitamin D
33000000 IU/kg, VE acetic acid esters 3.20 × 10
4mg/kg, menadione 5.20 × 10
3mg/kg, thiamine hydrochloride 2.60 × 10
3mg/kg, riboflavin 5.40 × 10
3mg/kg, puridoxine hydrochloride 2.20 × 10
3mg/kg, cyanocobalamin 25mg/kg, L-AA 2.10 × 10
5mg/kg, D-biotin 220mg/kg, niacinamide 2.50 × 10
4mg/kg, pantothenic acid 1.10 × 10
4mg/kg, folic acid 1.00 × 10
3mg/kg, methionine 30g/kg, lysine 10g/kg, xylo-oligosaccharide 3.60 × 10
4mg/kg, citric acid 3.00 × 10
5mg/kg, sodium glutamate 7.20 × 10
4mg/kg, residuals weight supplied by glucose.
5. a boar improves the preparation method of metabolic conditioning agent with anti-stress growth promoting, it is characterized in that: the concrete steps of described method are as follows:
Step 1, take various raw material by formula, with conditioning agent total amount, comprise following component: retinyl acetate 350 ~ 6,200,000 IU/kg, vitamin D
3300 ~ 4,600,000 IU/kg, VE acetic acid esters 2.5 × 10
4~ 3.8 × 10
4mg/kg, menadione 4.0 × 10
3~ 6.0 × 10
3mg/kg, thiamine hydrochloride 2.0 × 10
3~ 3.0 × 10
3mg/kg, riboflavin 4.5 × 10
3~ 6.5 × 10
3mg/kg, puridoxine hydrochloride 1.5 × 10
3~ 2.6 × 10
3mg/kg, cyanocobalamin 20 ~ 30mg/kg, L-AA 1.7 × 10
5~ 2.5 × 10
5mg/kg, D-biotin 175 ~ 264mg/kg, niacinamide 2.0 × 10
4~ 3.0 × 10
4mg/kg, pantothenic acid 0.85 × 10
4~ 1.32 × 10
4mg/kg, folic acid 0.80 × 10
3~ 1.2 × 10
3mg/kg, methionine 24 ~ 36g/kg, lysine 8 ~ 12g/kg, xylo-oligosaccharide 2.40 × 10
4~ 3.60 × 10
4mg/kg, citric acid 2.00 × 10
5~ 3.00 × 10
5mg/kg, sodium glutamate 4.80 × 10
4~ 7.20 × 10
4mg/kg, supplies weight by glucose;
Step 2, by vitamin D
3, cyanocobalamin, D-biotin mix, and obtains once mixture;
Step 3, once mixture, retinyl acetate, thiamine hydrochloride, puridoxine hydrochloride, folic acid are carried out second time mixing, obtain secondary mixture;
Step 4, by secondary mixture, menadione, riboflavin, pantothenic acid, niacinamide, VE acetic acid esters, xylo-oligosaccharide, carry out third time mixing, obtain three mixtures;
Step 5, three mixtures, sodium glutamate, citric acid, L-AA, methionine, lysines are carried out the 4th mixing, obtain four mixtures;
Step 6, add in four mixtures rated weight glucose carry out the 5th time mixing, namely obtain finished product.
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| US11426347B2 (en) * | 2017-03-28 | 2022-08-30 | Ajinomoto Co., Inc. | Food for improving intraintestinal environment |
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