CN103190545A - Gosling anti-stress synbiotics and traditional Chinese medicine composition and preparation method of composition - Google Patents
Gosling anti-stress synbiotics and traditional Chinese medicine composition and preparation method of composition Download PDFInfo
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- CN103190545A CN103190545A CN2013101293221A CN201310129322A CN103190545A CN 103190545 A CN103190545 A CN 103190545A CN 2013101293221 A CN2013101293221 A CN 2013101293221A CN 201310129322 A CN201310129322 A CN 201310129322A CN 103190545 A CN103190545 A CN 103190545A
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Abstract
The invention relates to the field of livestock rearing, and in particular relates to a micro-ecologic feed and a preparation method thereof. The gosling anti-stress synbiotics and traditional Chinese medicine composition is composed of a micro-ecologic synbiotics feed additive and Chinese herbal medicine additives, wherein the micro-ecologic synbiotics feed additive is composed of the following components in parts by weight: 10-60 parts of micro-ecologic probiotics and 40-90 parts of oligosaccharide, the viable count of bifidobacterium in each gram of the micro-ecologic synbiotics feed additive is 20-45 billion, the micro-ecologic probiotics is a bifidobacterium preparation, the oligosaccharide comprises fructooligosaccharide, xylooligosaccharide, isomalto-oligosaccharide and soybean oligosaccharide, and the micro-ecologic synbiotics feed additive accounts for 1%-2% of the total weight of the gosling anti-stress synbiotics traditional Chinese medicine composition; and the Chinese herbal medicine additives are composed of the following components in parts by weight: 20-35 parts of spina data seeds, 15-25 parts of water lily, 10-20 parts of purple cathoclines, and 10-20 parts of folium isatidis. The formulation provided by the invention is capable of obviously improving the anti-stress ability of gosling.
Description
Technical field
The present invention relates to the livestock rearing field, particularly micro-ecological feed and preparation method thereof.
Background technology
What is called stress (stress) refer to a kind of nonspecific reaction that animal shows the poor environment factor.These factors comprise: the flip-flop of living environment is as transportation, crowded and trample etc. when changeing group and resettlement; The flip-flop of the flip-flop of environmental condition such as the variation of weather, light application time and intensity; The flip-flop of the change of nutritional status such as the kind of feed, quality, quantity, feed formula is unreasonable, the long-term lacking of some nutritional labeling or excessive; Deterioration such as the cultivation density of living environment is too high, harmful gas concentration is excessive, the dysdipsia of searching for food, water quality inferiority, ventilation is bad, dust is many, pathogenic microorganism is many, sanitary condition difference etc.; The flip-flop of feeding and management program, as grab goose, docking, injection, controlled feeding, suddenly clear excrement etc.; Disease is undoubtedly stress factor, and dispensing also easily cause stress.The phosphamidon class medical instrument of high dose has peculiar smell, excitant and toxicity, and mordant disinfectant is used for that the drinking-water, spraying, sterilization of feeding process etc. easily causes stress; Stressor because of some vaccine and immunologic process thereof can both cause stress.The unexpected variation of the unexpected movement of object, unexpected noise, work clothes color (particularly red) etc. can cause all that livestock and poultry are excited and generation stress.In fact, all can cause that the livestock and poultry body does not feel like oneself and causes the factor of body damage all to can be described as stressor.
Young goose is that a class is to the highstrung animal of environmental change.Young goose is under the stress situation, often shows symptoms such as apocleisis, dysphoria, growth are slowed down, death rate rising.Because the uncontrollability of Changes in weather adds China raiser and generally take the pattern of high density intensive culture for pursuing cultured output, therefore, in various degree stress phenomenon inevitable takes place in breeding process growing chickens goose.Because stress, annual China's direct economic loss that young goose aquaculture is brought of giving is above 2,000,000,000 yuan.Therefore, develop a kind of effect significantly, stable, the young goose Antistress agent easy to use of effect has very important meaning.
At present, be used for livestock and poultry anti-stress medicine or additive, mostly be chemicals, the long-term use, not only can cause bad reactions such as the livestock and poultry body develops immunity to drugs, stress reaction, poisoning, and its harmful components residue in the livestock products such as meat, egg, milk, can directly be detrimental to health.Modern medicine study shows that antibiotic residue may cause the human DNA structure to be undergone mutation in the animal derived food, thereby causes serious consequences such as " disabling ", " teratogenesis ", " carcinogenic ".
Natural Chinese medicinal herb and little ecological symphysis unit's feed addictive having no side effect with its uniqueness, no hazard residue, non-stress, do not produce drug resistance, advantage such as free from environmental pollution, become the benefit-additives of production green animal food and emphasis and the focus of herding worker research, external approved uses as the feed medicated premix.Along with further developing of China's animal husbandry and animal products foreign trade.Must pay attention to the reasonable use of medicated premix.
Summary of the invention
The object of the present invention is to provide the composition of forming with a kind of little ecological symphysis unit's feed addictive and Chinese herbal feed, the number of viable height that described additive contains, the stress reaction that can treat young goose with the feed of this additive preparation.
For achieving the above object, technical scheme of the present invention is:
Young goose is anti-stress symphysis unit Chinese medicine composition, formed by little ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing the bifidobacteria viable bacteria number in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 200-450, described little ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and the soyabean oligosaccharides; Described little ecological symphysis unit feed addictive accounts for the anti-1-2% that stress symphysis unit Chinese medicine composition gross weight of described young goose, and described Chinese herb feed additive is composed of the following components by weight: spina date seed 20-35 part; Water lily 15-25 part; Purple cyathocline herb 10-20 part; Folium isatidis 10-20 part.
Further, described young goose is anti-stress symphysis unit Chinese medicine composition, and to contain the bifidobacteria viable bacteria number be 40,000,000,000 to every gram in the described little ecological symphysis unit feed addictive.
Further, described young goose is anti-stress symphysis unit Chinese medicine composition, described little ecological symphysis unit feed addictive account for described young goose anti-stress symphysis unit Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 30 parts of spina date seeds; 20 parts of water lilys; 15 parts of purple cyathocline herbs; 15 parts of folium isatidis.
Contain the anti-feed that stress symphysis unit Chinese medicine composition of described young goose.
Further, containing mass fraction in the described feed is that 0.5% described young goose is anti-stress symphysis unit Chinese medicine composition.
Another object of the present invention is to provide the preparation method of the anti-preparation that stress symphysis unit Chinese medicine composition of above-mentioned young goose, this method is simple to operate, and production cost is low, is applicable to large-scale production.
The preparation method of the anti-preparation that stress symphysis unit Chinese medicine composition of described young goose with the 4-6% inoculation that Bifidobacterium press culture volume, is 6.0-8.0 in the pH value, and 37 ℃ of constant temperature leave standstill cultivation 24-72 hour, get bifidobacterium preparations; The Bifidobacterium culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get the anti-preparation that stress symphysis unit Chinese medicine composition of young goose.
Further, among the described preparation method, described bifidobacterium preparations specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the Bifidobacterium culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, took out refrigerator and preserved, stand-by;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of Bifidobacterium culture volume, be inoculated in the Bifidobacterium culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; The taking-up refrigerator is preserved, and is stand-by; The Bifidobacterium culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermented and cultured
With the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the Bifidobacterium culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets nutrient solution;
The D centrifugal dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained nutrient solution cooling back, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, place under the 40-50 ℃ of condition and dry, get bifidobacterium preparations.
Further, among the described preparation method, Bifidobacterium culture medium prescription: 0.6 part of egg peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
Beneficial effect of the present invention is: 1) have the following advantages with single comparing with the first feed addictive of ecological symphysis: can strengthen immunity and the anti-stress ability of animal, reduce the incidence of disease, the few or not medication of medication is conducive to health of human body and environmental protection; Be fit to modernization industry and produce constant product quality; 2) Chinese herbal medicine in this prescription, spina date seed have analgesia, anticonvulsion, cooling effect; Water lily has wet brain calms the nerves, and the effect of hot nourishing the liver falls in the heat-clearing bushing; Purple cyathocline herb has clearing heat and detoxicating, anti-inflammation hemostasia, dehumidifying diuresis; Folium isatidis is mainly used in the heat poison and sends out diseases such as spot, erysipelas, abscess of throat, aphthae, sore and toxic; Use in conjunction can improve young goose anti-stress ability.
The specific embodiment
The anti-preparation that stress symphysis unit Chinese medicinal composition preparation of embodiment 1 young goose
One, taking by weighing oligosaccharides is that each 10Kg of FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides is standby;
Two, the preparation of bifidobacterium preparations:
Bacterial classification is the Bifidobacterium (Bifidobacterium) that freeze-drying is preserved; The Bifidobacterium culture medium calculates composed of the following components by weight: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums, pH6.8,115 ℃ of high pressure steam sterilization 30min;
1. enlarge kind of a daughter bacteria, get bacterial classification on the inclined-plane (as an oese) inoculation and go into to be equipped with the Bifidobacterium culture medium in vitro, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the first order seed bacterium, takes out refrigerator and preserves, stand-by;
2. the preparation of secondary seed bacterium: with 5% the ratio of first order seed bacterium in the volume of Bifidobacterium culture medium, be inoculated in the Bifidobacterium bacterium culture medium, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium;
3. produce the preparation of the Bifidobacterium culture medium of usefulness: take by weighing each composition in the composition ratio of Bifidobacterium culture medium and be dissolved in water, pour in the microorganism fermentation tank, in 115 ℃ of steam sterilizations 30 minutes;
4. inoculation: the Bifidobacterium culture medium of usefulness to be produced is chilled to about 37 ℃, adds the secondary seed bacterium under flame control, inoculates by the ratio of the 6-8% of Bifidobacterium culture volume, stirs;
5. cultivate: close all breather valves, 37 ℃ of constant temperature culture 60h;
6. centrifugal dehydration: in biological fermentation tank, take out nutrient solution, refrigerator is temporary, treat nutrient solution cooling back in 3000 rev/mins centrifugal 20 minutes, remove supernatant, getting 2 times of chaff powder of bacterium mud adding mixes, the disk of packing into places oven dry in 45 ℃ of air dry ovens, carries out the Bifidobacterium evaluation by the GB4789-2012 method, and calculate total viable count, pack, get bifidobacterium preparations, keep in Dark Place standby at shady and cool dry place.
The preparation of the anti-preparation that stress symphysis unit Chinese medicine composition of three young geese
1) little ecological symphysis unit's feed addictive prescription one (being called for short additive 1): calculate composed of the following components by weight: 50 parts of bifidobacterium preparations, 90 parts of FOSs, wherein, viable count is 30,000,000,000 in the ecological symphysis of the every gram unit feed addictive.
Young goose is anti-stress symphysis unit Chinese traditional medicine composition composition formula one (being called for short composition 1): additive 1 accounts for 1% of described composition 1 gross weight, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), and described Chinese herb feed additive is composed of the following components by weight: 20 parts of spina date seeds; 15 parts of water lilys; 20 parts of purple cyathocline herbs; 20 parts of folium isatidis.
2) little ecological symphysis unit's feed addictive prescription two (being called for short additive 2): calculate composed of the following components by weight: 60 parts of bifidobacterium preparations, 40 parts of soyabean oligosaccharides, wherein, viable count is 45,000,000,000 in the ecological symphysis of the every gram unit feed addictive.
Young goose is anti-stress symphysis unit Chinese traditional medicine composition composition formula two (being called for short composition 2): additive 2 accounts for 2% of described composition 2 gross weights, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 2), and described Chinese herb feed additive is composed of the following components by weight: 35 parts of spina date seeds; 25 parts of water lilys; 10 parts of purple cyathocline herbs; 10 parts of folium isatidis.
3) little ecological symphysis unit's feed addictive prescription three (being called for short additive 3): calculate composed of the following components by weight: 20 parts of bifidobacterium preparations, 80 parts of xylo-oligosaccharides, wherein, viable count is 40,000,000,000 in the ecological symphysis of the every gram unit feed addictive.
Young goose is anti-stress symphysis unit Chinese traditional medicine composition composition formula one (being called for short composition 3): additive 3 accounts for 1.5% of described composition 3 gross weights, surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 3), and described Chinese herb feed additive is composed of the following components by weight: 30 parts of spina date seeds; 20 parts of water lilys; 15 parts of purple cyathocline herbs; 15 parts of folium isatidis.Mouthfeel the best of this prescription.
Each component in the said herbal medicine additive, see " national Chinese herbal medicine compilation " (Xie Zongwan chief editor for details, the People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition.
Each component of formula ratio is mixed in proportion, make granule, so that quality control, preservation, transportation and use.
Embodiment 2 anti-stress effects
One, experiment grouping
1 experimental group
Be chosen at 300 young geese that occur the transport stress reaction in the transportation, freely ingest and contain the feed of embodiment 1 described composition 3.The mass percent of the anti-preparation that stress symphysis unit Chinese medicine composition of the young goose that adds in the described feed is 1%.
2 control groups
Be chosen at 50 young geese that occur the transport stress reaction in the transportation, edible normal diet.
Two, index detects
Blood is got in blood sample acquisition method oxter, and blood sample was taked behind 1 day, administration after respectively at preceding 1 day of administration, administration in 3 days.Every each blood sampling 0.5ml, separation of serum is put-20 ℃ of refrigerators, makes the serum biochemistry index test, finishes in 4 days.
Biochemical investigation method serum physiological and biochemical index send laboratory, the People's Hospital, Rongchang County to detect.
Serum glucose, T4, the urea nitrogen assay of the young goose of experimental group and control group see Table 1.By table 1 as seen, test group serum glucose, T4, urea nitrogen average are tending towards descending, and through the t check, with control group utmost point significant difference (P＜0.01) are arranged.
The variation of table 1 serum glucose, T4, urea nitrogen
The lymphocytic variation of T: test group with control group relatively, 3 days difference extremely significantly (P＜0.01) after preceding 1 day of T lymphocyte percentage administration and the administration.The results are shown in Table 2.
The influence of table 2 pair T lymphocyte percentage
%, sky
(
*：P＜0.01；
**P＜0.05)
Experimental group is continuously in edible one week of feed of containing embodiment 1 described composition 3.After control group and experimental group were under equal conditions raised January respectively, the death rate of control group was 32%, and the experimental group death rate is 6%.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (8)
1. young goose is anti-stress symphysis unit Chinese medicine composition, it is characterized in that, formed by little ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing the bifidobacteria viable bacteria number in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 200-450, described little ecological probio is bifidobacterium preparations, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and the soyabean oligosaccharides; Described little ecological symphysis unit feed addictive accounts for the anti-1-2% that stress symphysis unit Chinese medicine composition gross weight of described young goose, and described Chinese herb feed additive is composed of the following components by weight: spina date seed 20-35 part; Water lily 15-25 part; Purple cyathocline herb 10-20 part; Folium isatidis 10-20 part.
2. young goose according to claim 1 is anti-stress symphysis unit Chinese medicine composition, it is characterized in that, to contain the bifidobacteria viable bacteria number be 40,000,000,000 to every gram in the described little ecological symphysis unit feed addictive.
3. young goose according to claim 1 is anti-stress symphysis unit Chinese medicine composition, it is characterized in that: described little ecological symphysis unit feed addictive account for described young goose anti-stress symphysis unit Chinese medicine composition gross weight 1.5%, described Chinese herb feed additive is composed of the following components by weight: 30 parts of spina date seeds; 20 parts of water lilys; 15 parts of purple cyathocline herbs; 15 parts of folium isatidis.
4. the preparation method of the anti-preparation that stress symphysis unit Chinese medicine composition of each described young goose of claim 1-3, it is characterized in that, with the 4-6% inoculation that Bifidobacterium is pressed culture volume, be 6.0-8.0 in the pH value, 37 ℃ of constant temperature leave standstill to be cultivated 24-72 hour, got bifidobacterium preparations; The Bifidobacterium culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, cysteine 0.1-0.2 part, sodium chloride 0.1-0.5 part, glucose 1-1.5 part, hepar siccatum 0.1-0.2 part, aqueous medium 90-99 part, sterilization; The bifidobacterium preparations of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get the anti-preparation that stress symphysis unit Chinese medicine composition of young goose.
5. preparation method according to claim 4 is characterized in that, described bifidobacterium preparations specifically may further comprise the steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification inoculation and go into to be equipped with the Bifidobacterium culture medium in vitro, 37 ℃ of constant temperature leave standstill to be cultivated 24 hours, obtained the first order seed bacterium, stored refrigerated;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of Bifidobacterium culture volume, be inoculated in the Bifidobacterium culture medium of sterilizing and cooling off, 37 ℃ of constant temperature leave standstill cultivates 24h, obtains the secondary seed bacterium; Stored refrigerated; The Bifidobacterium culture medium is poured in the microorganism fermentation tank into sterilization and cooling;
The C fermented and cultured
With the ratio inoculation of step B gained secondary seed bacterium in the 6-8% of the Bifidobacterium culture volume of sterilizing and cooling off, stir, 37 ℃ of constant temperature leave standstill cultivates 48-72h, gets nutrient solution;
The D centrifugal dehydration
Supernatant in the centrifugal 20min of 3000r/min, is removed in step C gained nutrient solution cooling back, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, place under the 40-50 ℃ of condition and dry, get bifidobacterium preparations.
6. preparation method according to claim 5 is characterized in that: the Bifidobacterium culture medium prescription: 0.6 part of peptone, 0.3 part of beef extract, 0.15 part of cysteine, 0.5 part in sodium chloride, 1.2 parts of glucose, 0.2 part of hepar siccatum, 95 parts of aqueous mediums.
7. contain the anti-feed that stress symphysis unit Chinese medicine composition of the described young goose of claim 1.
8. feed according to claim 7 is characterized in that, contain mass fraction in the described feed and be 0.5% described young goose is anti-stress symphysis unit Chinese medicine composition.
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| CN105124286A (en) * | 2015-09-15 | 2015-12-09 | 管天球 | Ecological goose feed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105124286A (en) * | 2015-09-15 | 2015-12-09 | 管天球 | Ecological goose feed |
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