CN103169823B - Veterinary drug composition, application, preparation and preparation method for preventing and treating mycotoxin poisoning - Google Patents

Abstract

The invention discloses a veterinary drug composition as well as a preparation and a preparation method thereof and particularly relates to a veterinary drug composition for preventing and controlling mycotoxicoses as well as a preparation and a preparation method of the veterinary drug composition. The veterinary drug composition for preventing and controlling mycotoxicoses is prepared from the following raw materials in parts by weight: 5-8 parts of radix bupleuri, 3-6 parts of radix scutellariae, 4-7 parts of astragalus, 5-8 parts of rhizome alismatis and 4-6 parts of schisandra chinensis. The veterinary drug composition for preventing and controlling mycotoxicoses, provided by the invention, can be used for effectively improving or reversing pathological injury caused by the mycotoxicoses, reducing the death rate of animals eating the mycotoxicoses, and furthermore, playing a role in effectively preventing and controlling the mycotoxicoses of the animals.

Description

Veterinary drug composition, application, preparation and preparation method for preventing and treating mycotoxin poisoning

technical field

The invention relates to a veterinary drug composition, a preparation and a preparation method thereof, in particular to a veterinary drug composition, a preparation and a preparation method thereof for preventing and treating mycotoxin poisoning.

Background technique

Mold is a general term for filamentous fungi, which tend to form branched and luxuriant mycelia, but do not produce large fruiting bodies like mushrooms; they can be roughly divided into Asperquillus (Asperquillus), Penicillium (Penicillium) , Fusarum (Fusarum), etc. Mycotoxins are metabolites produced during the growth and reproduction of molds on grains, food and feed, such as fusarium toxins and aflatoxins. Mycotoxins are usually small molecular substances with a molecular weight of several hundred to several thousand Daltons, and they have no resistance and are thermally stable substances that will not be destroyed by simple heating, which often cause mold in livestock, poultry or humans Toxin poisoning phenomenon.

One mold can produce multiple mycotoxins, and the same mycotoxin can also be produced by several molds. So far, dozens of mycotoxins that are recognized as harmful to livestock and human health have been isolated; the mycotoxins that are common in feed products and cause serious damage to the breeding industry include aflatoxins (AF) , trichothecenes (including vomitoxin and T-2 toxin), zearalenone (ZEN), ochratoxin A (ochratoxin, OT), fumonisins (fumonisins, FUM) and Ergot toxin (ergotoxin, ERG) and so on.

The above-mentioned mycotoxins are commonly found in feed and feed materials, which often cause serious harm to livestock and poultry breeding and human health, and bring huge economic losses. Since the intake of feed by livestock and poultry is continuous during the breeding process of livestock and poultry, mycotoxins will continue to accumulate. Even if the mycotoxins in a single weight of feed are only trace components, they can also be accumulated after a period of time. reach pathogenic or lethal doses.

The existing methods for removing mycotoxins in animal feed generally use traditional mycotoxin adsorbents or biological mold removers; although the former adsorbent can adsorb part of mycotoxins, it can also adsorb nutrients in the feed; The latter biological mold remover is difficult to be popularized due to poor self-stability, heat resistance and acid resistance.

Another existing method is to use traditional Chinese medicine compound to prevent the lesions caused by mycotoxins. The existing traditional Chinese medicine compound for the prevention and treatment of mycotoxin poisoning is generally a traditional Chinese medicine composition that protects the liver and restores liver function. The toxins are combined, and then the toxins are transported into the liver for detoxification and then excreted from the body; the Chinese medicine compound can remove the mycotoxins that have been absorbed into the body, and its medicinal ingredients can also effectively fight against liver damage and other diseases caused by mycotoxins. However, so far, there is still no traditional Chinese medicine compound that can effectively alleviate or prevent mycotoxin poisoning, especially the poisoning caused by aflatoxin.

Aflatoxin is one of the important causes of mycotoxins in livestock and poultry at present, and its toxicity is often greater than other mycotoxins; at the same time, the pathogenic mechanism of aflatoxin is to directly cause substantial pathological damage to the liver of livestock and poultry , manifested as decreased growth performance, decreased digestive capacity, increased liver enzymes, increased oxidative stress, etc., and may even lead to liver cirrhosis or liver cancer. Therefore, aflatoxins pose a greater threat to the livestock and poultry industry than other mycotoxins.

Contents of the invention

In view of this, the present invention provides a veterinary drug composition for preventing and treating mycotoxins, which can effectively improve or reverse the pathological damage caused by mycotoxin poisoning in animals, reduce the mortality rate of animals after mycotoxins, and thus effectively prevent and treat mycotoxin poisoning in animals role.

In order to solve the above technical problems, the technical solution of the present invention is to adopt a veterinary drug composition for preventing and treating mycotoxin poisoning. The veterinary drug composition for preventing and treating mycotoxin poisoning is prepared from the following Chinese medicinal materials, and each raw material is calculated in parts by weight :

Bupleurum 5-8 parts by weight;

Scutellaria baicalensis 3-6 parts by weight;

Astragalus 4-7 parts by weight;

Alisma 5-8 parts by weight; and

Schisandra 4-6 parts by weight.

Preferably, the veterinary drug composition for preventing and treating mycotoxin poisoning is prepared from the following Chinese medicinal materials, and each raw material is calculated in parts by weight:

Bupleurum 6-7 parts by weight;

Scutellaria baicalensis 4-5 parts by weight;

Astragalus 5-6 parts by weight;

Alisma 6-7 parts by weight; and

Schisandra 5-6 parts by weight.

Compared with the prior art, the present invention is described in detail as follows:

The technical solution adopted in the present invention is a veterinary drug composition for preventing and treating mycotoxin poisoning, which is prepared from the following Chinese medicinal materials, and each raw material is counted in parts by weight:

Bupleurum 5-8 parts by weight;

Scutellaria baicalensis 3-6 parts by weight;

Astragalus 4-7 parts by weight;

Alisma 5-8 parts by weight; and

Schisandra 4-6 parts by weight.

The present invention mainly uses the aforementioned five kinds of Chinese medicinal materials as raw materials to prepare a veterinary drug composition for preventing and treating mycotoxins, and the main raw materials of Chinese medicinal materials are Bupleuri, Scutellaria baicalensis, Radix Astragali, Alisma and Schisandra.

According to the theory of traditional Chinese veterinary medicine, from the perspective of syndrome differentiation and treatment, mycotoxin poisoning in livestock and poultry can be diagnosed as damp heat beating the liver and gallbladder. Damp heat stagnates in the liver and gallbladder, resulting in poor liver qi, abnormal bile secretion and excretion, weakened spleen and stomach transportation and transformation, and loss of appetite. According to the theory of traditional Chinese medicine, its treatment principle is to soothe the liver and relieve stagnation, clear away heat and dampness. Therefore, such Chinese medicinal materials can be used for configuration.

The inventors conducted research on a large number of Chinese medicinal materials, and found that the composition prepared from the raw material components and contents of the traditional Chinese medicine described in the present invention can effectively prevent and treat mycotoxin poisoning in animals, especially aflatoxin poisoning.

The traditional Chinese medicine Bupleurum used in the present invention enters the liver and gallbladder meridian and is good at dredging diarrhea; Scutellaria baicalensis enters the gallbladder meridian and is good at clearing away heat and dampness; The composition prepared by the five kinds of traditional Chinese medicine raw materials can play a relatively obvious preventive effect on animal mycotoxin poisoning (for the actual effect, please refer to the subsequent specific examples).

The raw materials in the above-mentioned Chinese medicine formula and the parts by weight of each raw material are all obtained on the basis of a large number of experiments. Even if the raw materials are the same as the present invention, if the parts by weight of each raw material are different from the present invention, the obtained The drug effect result is just not ideal enough.

Further, the present invention preferably adopts the veterinary drug composition for preventing and treating mycotoxin poisoning to be prepared from the following Chinese medicinal materials, and each Chinese medicinal material is calculated in parts by weight:

Bupleurum 6-7 parts by weight;

Scutellaria baicalensis 4-5 parts by weight;

Astragalus 5-6 parts by weight;

Alisma 6-7 parts by weight; and

Schisandra 5-6 parts by weight.

It can be seen from the following specific examples that further preferred embodiments of the present invention can improve the efficacy of preventing and treating mycotoxin poisoning.

The second object of the present invention is to provide the application of the aforementioned veterinary drug composition in the preparation of medicines for the prevention or treatment of mycotoxin poisoning in livestock and poultry.

The aforementioned veterinary drug composition of the present invention can be used to prepare medicines for preventing or treating mycotoxin poisoning of livestock and poultry. Mycotoxin poisoning has good efficacy.

Further, the present invention preferably adopts aflatoxin as mycotoxin in the aforementioned applications. Aflatoxin is currently the primary cause of mycotoxin poisoning in livestock and poultry, and, as can be seen from the examples below, the veterinary drug composition of the present invention has better drug efficacy data for preventing and treating aflatoxin poisoning than other mycotoxins efficacy data.

The third object of the present invention is to provide a veterinary drug preparation for preventing and treating mycotoxin poisoning, said veterinary drug preparation comprising any one of the aforementioned veterinary drug compositions. When preparing any of the aforementioned veterinary drug compositions into veterinary drug preparations, the preparation method can adopt conventional methods of existing traditional Chinese medicine veterinary drug preparations, and meanwhile, its dosage form can also adopt conventional preparations of existing veterinary drug preparations.

Further, the present invention preferably adopts the dosage form of the veterinary drug preparation as granules. Granular preparations not only have the characteristics of large dispersion, fast absorption, moderate action, and low toxic and side effects of traditional Chinese medicine oral liquid, but also have high drug stability. In addition, the granule preparation can be mixed with water or mixed with animal feed during use, which is convenient for group administration in large-scale farms. Moreover, compared with the oral liquid, the granule preparation is convenient for production, storage and transportation; therefore, the preferred dosage form of the veterinary preparation in the present invention is a granule.

The fourth object of the present invention is to provide a method for preparing the aforementioned veterinary drug preparation for preventing and treating mycotoxin poisoning, which includes the following steps:

1) Add 15-25 times the weight of Bupleurum, Scutellaria, Astragalus, and Schisandra to water, decoct for 0.8-1 hour, filter to obtain a decoction, and concentrate the obtained decoction to a relative density of 1.21-1.25;

2) Soak Alisma alisma in ethanol for 2-3 hours, collect 6-8 times the percolation liquid at a flow rate of 4-5mL/min; concentrate to a relative density of 1.01-1.05;

3) Combine the concentrate obtained in 1) and 2) to obtain the extract;

4) Mix the extract obtained in 3) with sugar according to the volume/mass ratio of 1L/1kg of 1:3-4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxin poisoning. The dosage form of the preparation is Granules.

The present invention adopts the above-mentioned steps to prepare the veterinary drug preparation in the form of granules. The above-mentioned preparation method can effectively and fully extract the medicinal ingredients in each Chinese medicinal material from the obtained veterinary drug preparation, thereby improving the medicinal effect of the preparation.

The percolation liquid is obtained by adopting the percolation method in conventional extraction of traditional Chinese medicine, and the solvent is ethanol.

Further, in the present invention, the decoction in the step 1) is preferably divided into two times, each time for 0.8-1 h, and the two filtrates are combined after filtration to obtain a water decoction.

Further, in the present invention, it is preferred to soak Alisma in step 2) with 75% ethanol.

Further, the present invention preferably adopts the preparation method comprising the following steps:

1) Add Bupleurum, Scutellaria, Radix Astragali, and Schisandra to 20 times the weight of water, decoct twice for 0.8-1 hour each time, filter to obtain decoction, and concentrate the obtained decoction to a relative density of 1.21-1.25;

2) Soak Alisma alisma in 75% ethanol for 3 hours, collect 8 times the percolation liquid at a flow rate of 4-5mL/min; concentrate to a relative density of 1.01-1.05;

3) Combine the concentrate obtained in 1) and 2) to obtain the extract;

4) Mix the extract obtained in 3) with sugar at a volume/mass ratio of 1L/1kg of 1:4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxin poisoning, and the dosage form of the preparation is granules .

Detailed ways

In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below in conjunction with specific examples.

Comparative example 1

Take each raw material according to the following parts by weight:

Bupleurum 1 part

Scutellaria baicalensis 15 parts

Comparative example 2

Take each raw material according to the following parts by weight:

Comparative example 3

Take each raw material according to the following parts by weight:

Comparative example 4

Take each raw material according to the following parts by weight:

Comparative example 5

Take each raw material according to the following parts by weight:

Comparative example 6

Take each raw material according to the following parts by weight:

Comparative example 7

Take each raw material according to the following parts by weight:

Comparative example 8

Take each raw material according to the following parts by weight:

Comparative example 9

Take each raw material according to the following parts by weight:

Comparative example 10

Take each raw material according to the following parts by weight:

Comparative Example 11

Take each raw material according to the following parts by weight:

Comparative example 12

Take each raw material according to the following parts by weight:

Comparative example 13

Take each raw material according to the following parts by weight:

Each of Comparative Examples 1-13 was prepared according to the following preparation methods to prepare corresponding veterinary drug compositions for the prevention and treatment of mycotoxin poisoning. The steps of each preparation method are as follows:

method 1):

Simply pulverize and mix the traditional Chinese medicines described in Comparative Examples 1-10 to obtain a veterinary drug composition.

Method (2):

1) Add bupleurum, scutellaria baicalensis, astragalus, and schisandra described in Comparative Example 7-13 to 15-25 times the weight of water, decoct for 0.8-1 hour, filter to obtain a decoction, and concentrate the obtained decoction to a relative density 1.21-1.25;

2) Soak Alisma alisma in 60% ethanol for 2-3 hours, collect 6-8 times the amount of percolate, and the flow rate is 4-5mL/min; the obtained percolate is concentrated to a relative density of 1.01-1.05;

3) Combine the concentrate obtained in 1) and 2) to obtain the extract;

4) Mix the extract obtained in 3) with sugar at a volume/mass ratio of 1:3-4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxins, and the preparation is in the form of granules.

Method (3):

1) Add the bupleurum, baicalin, astragalus, and schisandra described in Comparative Examples 7, 9, 11, and 13 to 20 times the weight of water, decoct twice, each time for 0.8-1h, filter and combine twice to decoct in water liquid, and the obtained decoction is concentrated to a relative density of 1.21-1.25;

2) Soak Alisma alisma in 75% ethanol for 3 hours, collect 8 times the amount of percolate at a flow rate of 4-5mL/min; the obtained percolate is concentrated to a relative density of 1.01-1.05;

3) Combine the concentrate obtained in 1) and 2) to obtain the extract;

4) Mix the extract obtained in 3) with sugar at a volume/mass ratio of 1:4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxins, and the preparation is in the form of granules.

Example 1——Efficacy test of the veterinary drug compositions of Comparative Examples 1-10 prepared according to method (1)

experimental method:

1.1 The basal ration was formulated with reference to the nutritional requirements of broiler chicks from the American NRC (1994). The formula and main nutritional indicators are shown in Table 1.

Table 1 Experimental basal diet composition and nutritional indicators

Multidimensional trace elements: 1500IU vitamin A, 200IU vitamin D3, 10mg vitamin E, 0.5mg vitamin K, 4mg vitamin Bz, 10mg d-pantothenic acid, 25mg vitamin B6, 25mg niacin, 800mg choline chloride, 0.15mg biotin, 60mg Mn, 40mg Zn, 80mg Fe, 8.0mg Cu, 0.35mgI, 0.15mg Se.

1.2 Animal grouping is feeding experiment

Select 1-day-old healthy broiler chickens, purchased from Sichuan Zhengda Farm, and divide them into 12 groups according to the requirements of the feeding experiment using a 2x2 factor completely random design, labeled as T1, T2, T3, T4, T5, T6, T7, T8, T9 , T10, T11, T12, a total of 12 experimental groups, each group set up 3 replicates, each replicate male and female half and half; the basic diet was fed to 7 days old, and the weak ones were eliminated.

T12 group: 8-27 days old were fed with basic diet (excluding aflatoxin B1), and T12 group was used as the blank control group;

Groups T1-T10: 8-21 days of age were uniformly fed with basal diet containing ochratoxin A 50 μg/kg; Taking the medicine, T1-T10 were administered by intragastric administration once a day according to the veterinary drug composition obtained in Comparative Example 1-10 respectively;

Group T11: From 8 to 21 days old, the basal diet containing ochratoxin A 50 μg/kg was uniformly fed, and from 22 days to 27 days, only the basal diet (without ochratoxin A) was fed.

The above-mentioned T1-T12 groups were routinely vaccinated against fowl plague, chicken viral hepatitis, and avian influenza. Raised on the ground, free access to food and water, 24h of light, the test period of 27d (1 to 27 days old).

Ochratoxin A is produced by fermenting Ochratoxin 2471 (CICC code, purchased from China Industrial Microorganism Collection Center) according to the method of Marquardt et al. Ochratoxin A in corn and feed was determined by high performance liquid chromatography.

1.3 Slaughter test and sample collection

After the feeding experiment, samples were taken at 21, 23, 25, and 27 days of age for dynamic treatment observation. Broilers with similar body weight were selected for each group, given water but no feed, fasted for 12 hours, and weighed. Vacuum blood collection tubes without anticoagulant in the jugular vein, the serum was naturally precipitated, divided into Eppendof tubes, and stored in a -20°C refrigerator. Immediately take part of the liver tissue for analysis of hepatic cell apoptosis by flow cytometry, and another part of the liver tissue is fixed in 10% neutral formaldehyde for pathological tissue sectioning.

1.4 Analysis of serum biochemical indicators

Alanine aminotransferase (GPT), aspartate aminotransferase (GOT), alkaline phosphatase (AKP), glutathione-S transferase (GST), using the kit provided by Nanjing Jiancheng Bioengineering Institute in Bio-rad680 enzyme labeling instrument and UV spectrophotometer.

1.5 Determination of serum antioxidant indexes

Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) were tested on Bio-rad680 microplate reader and ultraviolet spectrometer using kits provided by Nanjing Jiancheng Bioengineering Institute. Photometer measurement.

1.6 Data processing

Experimental data processing and analysis adopted the general linear model (General Linear Models Procedure) analysis of variance in SPSS (17.0). When the difference was significant, Duncan's method was used to carry out multiple comparisons between the means of each group. P<0.05 indicated that the difference was significant. express.

2 results

2.1 Serum biochemical indicators

Table 2 - Alkaline Phosphatase Determination Data

Note: The same vertical shoulder mark * means significant difference compared with 1-T11 group (P<0.05); the same vertical shoulder mark ** means extremely significant difference compared with 1-T11 group (P<0.01).

Table 3 - Determination data of superoxide dismutase

Note: The same vertical shoulder mark * means significant difference compared with 1-T11 group (P<0.05); the same vertical shoulder mark ** means extremely significant difference compared with 1-T11 group (P<0.01).

From the measurement data in Table 2 and Table 3 above, it can be seen that the efficacy data of the veterinary drug composition for preventing and treating mycotoxins prepared by Comparative Example 1-6 according to the preparation method (1) is significantly lower than that of Comparative Example 7- 10 Pharmacodynamic data of the veterinary drug composition for preventing and treating mycotoxins prepared according to the preparation method (1).

Example 2—Efficacy test of the veterinary drug preparations of Comparative Examples 7-13 prepared according to method (2)

The experimental method and steps of Example 2 are the same as in Example 1, only the experimental toxin is replaced by aflatoxin B1, and the experimental object is replaced by Cherry Valley commercial meat duck, purchased from Sichuan Mianyang Cherry Valley Duck Seed Industry Company, so as to carry out the same medicine Effect test, each experimental group is marked as 2-T1, 2-T2, 2-T3, 2-T4, 2-T5, 2-T6, 2-T7, 2-T8, 2-T9, among them, 2-T1 ——2-T7 is the experimental group that adopts the veterinary drug preparation that control example 7-13 makes respectively, and its feeding method is the same as 1-T1——1-T10 in embodiment 1; 2-T8 is the blank control group, and its feeding method The method is the same as that of 1-T12 in Example 1, and 2-T9 is the poisoning experiment group, and the feeding method is the same as that of 1-T11 in Example 1. Examples of measured results are as follows:

Table 4 - Determination data of alanine aminotransferase

Note: The same vertical shoulder mark * means significant difference compared with 2-T9 group (P<0.05); the same vertical shoulder mark ** means extremely significant difference compared with 2-T9 group (P<0.01).

The production of aflatoxin is formed by fermentation of Aspergillus flavus strain 2219 (CICC number, purchased from China Industrial Microorganism Collection Center) according to the method of Odette L. Aflatoxin B1 in corn and feed was determined by high performance liquid chromatography.

It can be seen from Table 4 above that in the results of the determination of the efficacy of the veterinary drug preparations prepared according to the preparation method (2) of Comparative Examples 7-13 for the prevention and treatment of mycotoxins - aflatoxins, Comparative Examples 11-13 were significantly higher than those of the Control Example 7-10.

Example 3—Efficacy test of the veterinary drug preparations of Comparative Examples 7, 9, 11, and 13 prepared according to method (3)

The experimental method and steps of Example 3 are the same as those in Example 1. The experimental toxins were set to aflatoxin B1 and ochratoxin A respectively, and the experimental subjects were changed to Cherry Valley commercial meat ducks, which were purchased from Sichuan Mianyang Cherry Valley Duck Seed Company. To carry out the same drug efficacy test; each experimental group is marked as aflatoxin B1 experimental group: 3-T1, 3-T2, 3-T3, 3-T4, 3-T5, 3-T6 and ochratoxin A experiment Groups: 4-T1, 4-T2, 4-T3, 4-T4, 4-T5, 4-T6; among them, 3-T1——3-T4 and 4-T1——4-T4 are the control examples respectively 7, 9, 11, and 13 prepared experimental groups of veterinary drug preparations, the feeding method is the same as that of 1-T1——1-T10 in Example 1; 3-T5 and 4-T5 are blank control groups, and the feeding method is the same as In Example 1, 1-T12; 3-T6 and 4-T6 are poisoning experimental groups, and the feeding method is the same as that of 1-T11 in Example 1. Examples of measured results are as follows:

Aflatoxin B1 experimental group:

Table 5 - Determination data of alanine aminotransferase

Note: The same vertical shoulder mark * means significant difference compared with 3-T6 group (P<0.05); the same vertical shoulder mark ** means extremely significant difference compared with 3-T6 group (P<0.01).

Ochratoxin A experimental group:

Table 6 - Determination data of alanine aminotransferase

Note: The same vertical shoulder mark * means significant difference compared with 4-T6 group (P<0.05); the same vertical shoulder mark ** means extremely significant difference compared with 4-T6 group (P<0.01).

It can be seen from Table 5 and Table 6 that the veterinary drug composition and veterinary drug preparation of the present invention have better control effects on aflatoxin than other mycotoxins.

The above are only preferred implementations of the present invention, and it should be noted that the above preferred implementations should not be regarded as limiting the present invention, and the scope of protection of the present invention should be based on the scope defined in the claims. For those skilled in the art, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (9)

1. A veterinary drug composition for preventing and treating mycotoxin poisoning, characterized in that: the veterinary drug composition for preventing and treating mycotoxin poisoning is prepared from the following Chinese medicinal materials, and each raw material is calculated in parts by weight: 2. The veterinary drug composition for preventing and treating mycotoxin poisoning according to claim 1, characterized in that: the veterinary drug composition for preventing and treating mycotoxin poisoning is prepared from the following Chinese medicinal materials, and each raw material is calculated in parts by weight: 3. The application of the veterinary drug composition according to any one of claims 1-2 in the preparation of medicines for preventing or treating livestock and poultry mycotoxin poisoning. the 4. The application according to claim 3, characterized in that: the mycotoxin is aflatoxin. the 5. A veterinary drug preparation for preventing and treating mycotoxin poisoning, characterized in that: the veterinary drug preparation comprises the veterinary drug composition according to any one of claims 1-2. the 6. The veterinary drug preparation according to claim 5, characterized in that: the dosage form of the preparation is granules. the 7. The preparation method of the veterinary drug preparation for preventing and treating mycotoxin poisoning according to claim 6, characterized in that it comprises the following steps: 1) Add 15-25 times the weight of Bupleurum, Scutellaria, Astragalus, and Schisandra to water, decoct for 0.8-1 hour, filter to obtain a decoction, and concentrate the obtained decoction to a relative density of 1.21-1.25; 2) Soak Alisma alisma in ethanol for 2-3 hours, collect 6-8 times the amount of percolation liquid at a flow rate of 4-5mL/min; concentrate to a relative density of 1.01-1.05; 3) Combine 1) and 2) the obtained concentrated solution to obtain extract; 4) Mix the extracted extract obtained in 3) with sugar according to the ratio of L/kg volume/mass ratio of 1:3-4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxin poisoning, and the dosage form of the preparation is Granules. the 8. The preparation method according to claim 7, characterized in that: the decocting in the step 1) is divided into two times, each time for 0.8-1h, after filtering, the two filtrates are combined to obtain a water decoction. the 9. The preparation method according to claim 7, characterized in that: in the step 2), Alisma is soaked with 75% ethanol. the 10. The preparation method according to claim 7, characterized in that it comprises the following steps: 1) Add Bupleurum, Scutellaria, Radix Astragali, and Schisandra with 20 times the weight of water, decoct twice, each time for 0.8-1h, filter to obtain a decoction, and concentrate the obtained decoction to a relative density of 1.21-1.25; 2) Soak Alisma alisma in 75% ethanol for 3 hours, collect 8 times the percolation liquid at a flow rate of 4-5mL/min; concentrate to a relative density of 1.01-1.05; 3) Combine 1) and 2) the obtained concentrated solution to obtain extract; 4) Mix the extract obtained in 3) with sugar according to the ratio of L/kg volume/mass ratio of 1:4, sieve, and dry to obtain the veterinary drug preparation for preventing and treating mycotoxin poisoning, and the dosage form of the preparation is granule . the