CN103155853A - Technical method of creating parent of polygene type colony heterosis utilization - Google Patents
Technical method of creating parent of polygene type colony heterosis utilization Download PDFInfo
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- CN103155853A CN103155853A CN2011104344866A CN201110434486A CN103155853A CN 103155853 A CN103155853 A CN 103155853A CN 2011104344866 A CN2011104344866 A CN 2011104344866A CN 201110434486 A CN201110434486 A CN 201110434486A CN 103155853 A CN103155853 A CN 103155853A
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Abstract
A technical method of creating a parent of polygene type colony heterosis utilization is a new technology of breeding the parent of the polygene type colony heterosis utilization. The technical method of creating the parent of the polygene type colony heterosis utilization can effectively polymerize merits of resource materials. The technical method of creating the parent of the polygene type colony heterosis utilization includes the following steps of constructing a recombinant inbred line through two subject parents, wherein the two parents hybridization F1 is obvious in superiority and preferably one of the two parents is a local main cultivar, selecting a monophyly from an F5 generation, wherein agriculture characters of the monophyly is basically consistent with that of a female parent, screening a monophyly from an F6 generation through a markers assisted selection technology, wherein genetic background of the monophyly is approximate to a male parent, paring the monophyly screened from the F6 generation and the original female parent, identifying characters including heterosis, resistance, uniformity, quality and the like of an F2 genration, and breeding a new material, wherein the new material and the female parent have complementary advantages, the plant type of the new material is ideal, the resistance of the new material is wide, the quality of the new material is good, characters influencing the colony regularity are all shown consistently, the genetic background difference of the new material is large, and heterosis of the F2 generation is prominent in superiority. The technical method of creating the parent of the polygene type colony heterosis utilization effectively solves the problem that parent selection of the polygene type colony heterosis utilization is difficult and enables teleonomy of the parent breeding to be more explicit and controllability of the parent breeding to be stronger.
Description
Affiliated technical field
Patent of the present invention belongs to Japonica Hybrid genetic breeding field, is a kind of molecular marker assisted selection Japonica Hybrid parent that relates to, and promotes the breeding technique of Japonica Hybrid propagation and employment.
Background technology
The development result of Japonica Hybrid is remarkable in recent years, still remains to be improved but compare with the development speed of indica Hybrid Rice.The reason of restriction Developing Japonica Hybrid Rice is many-sided, and wherein hybrid vigour problem and Seed production of hybrid japonica rice production problems are the principal elements that affects the Japonica Hybrid application.Proposition and the enforcement of " japonica rice polygene type colony heterosis utilization " technical method have improved heterotic utilization to a certain extent, have most importantly solved the problem that Seed production of hybrid japonica rice yields poorly, cost is high, have a big risk.For the development of Japonica Hybrid provides new thinking and reference.But now the parent of " polygene type colony heterosis utilization " selects, it is mainly mass-election from existing kind, to select like this meet consistent, the basic indifference of various economical characters breeding time, genetic background differs greatly and the light temperature is very large than the material difficulty of insensitiveness, the development of this new technology of restriction to a great extent.Therefore, parent's the bottleneck problem that is selected to this technical development and application.
Summary of the invention
invented a kind of seed selection " polygene type colony heterosis utilization " parent's new technology, this technology has changed the method for mass-election parent in existing kind, but autotelic initiative parent, (it is with the obvious advantage that two parents are hybridized F1 by two main body parents, one of them is preferably local main breed) the structure RIL, F5 is for selecting the monosystem basically identical with maternal economical character, F6 is for filtering out genetic background near the monosystem of male parent with Molecular Marker Assisted Selection Technology again, again with former maternal combo, to the F2 advantage, resistance, regularity, the proterties such as quality are identified, have complementary advantages thereby select with female parent, plant type is desirable, resistance is extensive, best in quality, the proterties that affects colony's regularity all shows extremely consistent, genetic background difference is large, the outstanding new material of hybridization F2 advantage, use with development for the Rapid Implementation of " polygene type colony heterosis utilization " new technology the more parent material of horn of plenty is provided.
The technical solution adopted for the present invention to solve the technical problems is:
1. select the rice varieties (or strain) of different hereditary basiss to carry out between kind or the hybridization of the artificial emasculation of subspecies, screening output, quality, resistance etc. have the combination of advantage;
2. plant and plant hybrid combination F1, mix results F1 seed plantation F2, according to the breeding objective of determining, in aspect comprehensive selection such as target breeding time, plant type, output, rice matter, ripening rate, resistances, mixedly receive 10,000 in the F2 segregation population;
3. the direct-seeding planting previous generation mixed F3 colony of receiving is no less than 4000 residue backups, continues to select according to breeding goal, eliminates plant height, breeding time and departs from larger individual plant, mixes results F4 seed and is no less than 10,000;
4. continue in Hainan the mixed F4 of receipts of live previous generation colony and be no less than 4000 residue backups, set up contrast breeding time, continue to select according to breeding goal, mixedly receive every strain and receive 2-3 grain seed.
5. plant and plant season 4000, the mixed F5 seed of receiving, take to get single transplantation of seedlings, increase seeding row spacing, at F5 for the maturing stage focal selection, will be under the prerequisite that keeps high yield, high-quality, better resistance, require selected system and maternal breeding time within the front and back of contrast breeding time 3 days, and meet the target application ecotope breeding time, each economical character must be consistent with female parent, each monosystem is collected two fringes.
6. tassel of Tianjin plantation, stay a tassel backup.6 leaves are carried DNA during the phase, the molecular labeling classification is divided into maternal class, the male parent class.Select genetic background near male parent, phenotype is near monosystem and the former maternal preparing hybrid kind (artificial emasculation hybridization) of female parent.
7, crossbreed Hainan adds generation, is respectively to mix respectively to receive the solid seed of F1.Positive season is established 3 repetitions to target application ecotope plantation F2 colony, repeats to be no less than 100 square metres at every turn.Take local main breed as contrast, the indexs such as each F2 colony regularity, yield heterosis, resistance, Mi Zhi are identified the product ratio, select best monosystem as new parent system.
8. the parental stock expansion is numerous, and large seeding row spacing list transplantation of seedlings is in time removed the larger strain of variation in heading stage, pustulation period, mixes sowing after maturation.Stored refrigerated.
The invention has the beneficial effects as follows, the one: the present invention in implementation process, merit that can the polymerization resource material.The 2nd: the parent who efficiently solves " polygene type colony heterosis utilization " selects hard problem, and make the purpose of parent's seed selection clearer and more definite, can be handling stronger.The 3rd: utilize Molecular Marker Assisted Selection Technology, can filter out very rapidly phenotype similar, the material of genetic background significant difference, the preparation of making up for advantage provides the hereditary basis reference.
Embodiment:
The present invention is further described below in conjunction with embodiment.
1, in August, 05 (Tianjin) Liaoxing No.1/D100 (artificial emasculation hybridization), gather in the crops 50 crossbreed.
2, late September Hainan plantation crossbreed (F1) 20 strains then.
3, in April, 06,10,000, the solid seed of F1 was received in Hainan, wherein 4000 live (30m2), the remaining preservation.
4, in July, 06, the solid seed of F2 plant was collected in Hainan, and each individual plant is received the 2-3 grain, live 4000/30m2, the remaining preservation.
5, the solid seed of in November, 06 Hainan results F3 plant, each individual plant is received the 2-3 grain, wherein 4000 individual plant rice transplantings, the remaining preservation inserted simultaneously L67 and done contrast.
6, in April, 07, collect plant type like each two tassel (F5) of individual plant of Liaoxing No.1.Summer in 2007, each individual plant was planted a tassel to Tianjin, stayed a tassel.6 leaves are carried DNA during the phase, the molecular labeling classification is divided into maternal class (Liaoxing No.1 class), male parent class (D100 class).Select genetic background near D100, phenotype is near the monosystem L100 of Liaoxing No.1.Prepare then Liaoxing No.1/L100 (artificial emasculation hybridization) seed August, 100 of results crossbreed.
7, the winter in 2007, plantation 50 hybridization grains in Hainan add generation, the mixed solid seed of true hybrid of receiving.
8, the summer in 2008, in areas of Shenyang plantation F2 colony, establish three repetition, Liaoxing No.1 compares, and F2 colony shows neat and consistent, and yield heterosis is obvious, and resistance (blast resisting) is than the obvious improve of Liaoxing No.1, and is best in quality.
9, L100 formally puts into production use as parent material.
Claims (2)
1. technical method of formulating the polygene type heterosis utilization parent of colony is characterized by:
(1) select the rice varieties (or strain) of different hereditary basiss to carry out between kind or the hybridization of the artificial emasculation of subspecies, screening output, quality, resistance etc. have the combination of advantage;
(2) build RIL
(3) plantation upper season mixed receipts 4000, F5 seed, take to get single transplantation of seedlings, increase seeding row spacing, at F5 for the maturing stage focal selection, will be under the prerequisite that keeps high yield, high-quality, better resistance, require selected system and maternal breeding time within the front and back of contrast breeding time 3 days, and meet the target application ecotope breeding time, each economical character must be consistent with female parent, each monosystem is collected two fringes.
(4) tassel of Tianjin plantation stays a tassel backup.6 leaves are carried DNA during the phase, the molecular labeling classification is divided into maternal class, the male parent class.Select genetic background near male parent, phenotype is near monosystem and the former maternal preparing hybrid kind (artificial emasculation hybridization) of female parent.
(5) crossbreed Hainan adds generation, is respectively to mix respectively to receive the solid seed of F1.Positive season is established 3 repetitions to target application ecotope plantation F2 colony, repeats to be no less than 100 square metres at every turn.Take local main breed as contrast, the indexs such as each F2 colony regularity, yield heterosis, resistance, Mi Zhi are identified the product ratio, select best monosystem as new parent system.
(6) the parental stock expansion is numerous, and large seeding row spacing list transplantation of seedlings is in time removed the larger strain of variation in heading stage, pustulation period, mixes sowing after maturation.Stored refrigerated.
The present invention different from the past in current material mass-election parent's method, but autotelic initiative parent material.Be embodied in following some: (1) the present invention in implementation process, merit that can the polymerization resource material.(2) parent who efficiently solves " polygene type colony heterosis utilization " selects hard problem, and make the purpose of parent's seed selection clearer and more definite, can be handling stronger.(3) utilize Molecular Marker Assisted Selection Technology, can filter out very rapidly phenotype similar, the material of genetic background significant difference, the preparation of making up for advantage provides the hereditary basis reference.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115316266A (en) * | 2022-09-05 | 2022-11-11 | 新疆农垦科学院 | Crop population improvement and variety breeding method |
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| US20030115624A1 (en) * | 2000-03-10 | 2003-06-19 | Terry L. Irene | Quantitative trait loci for insect resistance in soybeans |
| US6884931B2 (en) * | 2002-08-30 | 2005-04-26 | Ricetec, Inc. | Rice hybrid RH101 |
| CN1568665A (en) * | 2004-04-26 | 2005-01-26 | 广东省农业科学院水稻研究所 | Seed selection method of paddy in population and variety |
| CN101268757A (en) * | 2008-04-02 | 2008-09-24 | 江苏省农业科学院 | Excellent flavour rice breeding method |
| CN102027880A (en) * | 2009-09-28 | 2011-04-27 | 天津天隆农业科技有限公司 | Rice variety breeding method partially utilizing hybrid vigor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115316266A (en) * | 2022-09-05 | 2022-11-11 | 新疆农垦科学院 | Crop population improvement and variety breeding method |
| CN115316266B (en) * | 2022-09-05 | 2023-09-08 | 新疆农垦科学院 | Crop population improvement and variety breeding method |
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Application publication date: 20130619 |