CN103145459A - Process for preparing liquid fertilizer by using enzymolysis and fermentation of animal bloods and taking oil supply angle as defoaming agent - Google Patents
Process for preparing liquid fertilizer by using enzymolysis and fermentation of animal bloods and taking oil supply angle as defoaming agent Download PDFInfo
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- CN103145459A CN103145459A CN2013101086607A CN201310108660A CN103145459A CN 103145459 A CN103145459 A CN 103145459A CN 2013101086607 A CN2013101086607 A CN 2013101086607A CN 201310108660 A CN201310108660 A CN 201310108660A CN 103145459 A CN103145459 A CN 103145459A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a process for preparing a liquid fertilizer by using the enzymolysis and fermentation of animal bloods and taking an oil supply angle as a defoaming agent, and belongs to the technical field of bioengineering. For solving the problems that in the past, the bloods poured from slaughterhouses in the process of red overglazing are all used as a raw material for producing feed additives, once the bloods are not cleaned and transported timely, the potential safety hazards of feeds caused by the decomposition of the bloods, the environmental pollution caused by organic wastewater, and the economic losses are caused, the invention provides a process for preparing a liquid fertilizer by using the enzymolysis and fermentation of animal bloods and taking an oil supply angle as a defoaming agent, and the process is implemented by taking animal bloods (pig blood, cattle blood, sheep blood and donkey blood and the like) as the main raw materials of a fermentation substrate and being supplemented by brown sugar, white sugar, an oil supply angle, protease, associated strains, wherein the oil supply angle especially achieves an important effect of preventing the losses of materials caused by overflowing in the process of heating and sterilization of raw and auxiliary materials, thereby avoiding the secondary pollution of the environment, and meanwhile, the organic phosphorus content is increased.
Description
Technical field
The invention belongs to technical field of bioengineering, relate to a kind of animal blood enzymatic hydrolysis and fermentation that utilizes and prepare liquid fertilizer technique with footwork and take oily angle as defoamer.
Background technology
China be in the world meat production and consumption big country, in recent years, along with the raising day by day of people's living standard, fowl poultry kind food demand amount increases year by year, thereby has driven developing on an unprecedented scale of aquaculture.The annual slaughtering amount of China is large, and blood resource is very abundant.Control blood was out fallen all as the raw material of producing fodder additives smearing red operation in the slaughterhouse in the past, in case clear the untimely corrupt problem that will cause feed safety hidden danger and organic waste water contaminate environment and financial loss that occurs.
Summary of the invention
Fall control blood out all as the raw material of producing fodder additives in order to solve slaughterhouse in the past smearing red operation, untimelyly occur corrupt and feed safety hidden danger and organic waste water contaminate environment and financial loss problem that cause in case clear, the invention provides and a kind ofly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer.This technology utilization animal blood (pig blood, ox blood, sheep blood, donkey blood etc.) is the main raw material of fermentation substrate, be aided with brown sugar, white sugar, You Jiao, proteolytic enzyme, mixed strains, wherein especially play aspect the froth breaking of oily angle in supplementary material intensification sterilization process and prevent that material from overflowing loss, avoid the vital role of secondary environmental pollution, increase simultaneously organophosphorus content.
The present invention utilizes animal blood (pig blood, ox blood, sheep blood, donkey blood etc.) to be the main raw material of fermentation substrate, be aided with brown sugar, white sugar, You Jiao, proteolytic enzyme, mixed strains, adopt enzymatic hydrolysis and fermentation simultaneous techniques degraded slaughterhouse animal blood and organic waste water to produce organic fertilizer, concrete steps are as follows:
1, get 1000 kilograms of animal bloods (pig blood, ox blood, sheep blood, donkey blood etc.) and be placed in sterilization tank;
2, get brown sugar 100-200kg, white sugar 100-200kg, add the slaughterhouse to clean drop blood groove organic waste water and make mixed solution 800-1500kg;
3, the syrup mixed solution is squeezed in sterilization tank, with the animal blood mix and blend, be warming up to 95 ± 5 ℃, sterilized 0.5-1 hour; Add successively when temperature rises to more than 85 ℃ and account for the oily angle stirring froth breaking that mixes rear material gross weight 0.5-1.0%;
4, be cooled to 50 ± 2 ℃, move to fermentor tank; In tank through 30 minutes, 115 ℃ of High Temperature Sterilizations.
5, add animal proteolytic enzyme 2.5-5.4kg, in tank, temperature is controlled at 50 ± 2 ℃;
6, after enzymolysis 4-6 hour, be cooled to 38 ± 2 ℃, adding the mass ratio of the outstanding fourth pichia spp of bacterial classification mixed solution 10-15kg(, Bacillus licheniformis and lactobacillus rhamnosus is 2-5:5-10:2-5) stir, carry out aerobic, anaerobic stages fermentation.
7, interim leavening temperature is connected with the oxygen supply time changing curve and is opened the filtration uncontaminated air pipeline valve that connects tank body, 38 ℃ ± 2 ℃ temperature continue enzymolysis below, limit aerobic fermentation 10-12 hour, are cooled to 30 ℃ ± 2 ℃, close and filter uncontaminated air valve, 13-15 hour anaerobically fermenting.
8, more than sampling survey viable count reaches 200,000,000/mL, survey pH value 5-6, Dan Lin Jia＞=4% can be cooled to 20 ℃ or room temperature, carries out can.
The present invention fully degrades the protein in blood by adding animal proteolytic enzyme to carry out enzymolysis and outstanding fourth pichia spp, Bacillus licheniformis and the compound viable bacteria of lactobacillus rhamnosus are fermented.This compound viable bacteria is relatively stable, inulinase-producing activity is high, can utilize protein in blood and auxiliary material, carbohydrate etc. carries out growth and breeding, form dominant microflora, and effectively suppress the breeding of multiple spoilage organism, after fermentation, amino peptide nitrogen content, Soluble Protein Contents, organophosphorus etc. all obviously increase.Due in the composition of animal blood except the oxyphorase degraded product, also contain the active substance that appropriate minerals and vitamins and other physiological metabolism produce, especially contain a large amount of Yelkin TTS in oily angle, the common microbial organic fertilizer that forms useful crop root and vine growth and development after degraded.
The liquid composite microbial organic fertilizer of this explained hereafter can as the starter of solid biofertilizer, also can dilute 350-500 times of liquid irrigating root foliage-spray and replenish crop alimentary.Activating soil improves ground temperature, administers the soil compaction that causes due to the life-time service chemical fertilizer, promotes crop root flourishing, improves resistance against diseases.Especially suitable tobacco and fruit tree and greengrocery are used.
Embodiment
Elaborate technical scheme of the present invention below in conjunction with embodiment; but do not limit protection scope of the present invention; any those of ordinary skill in the art are in the technical scope that the present invention discloses, and the variation that can expect easily or replacement are within all should being encompassed in protection scope of the present invention.
The present invention prepares liquid microbial organic fertilizer in accordance with the following steps:
One, bacterium solution preparation:
1.1 lactobacillus rhamnosus bacterium liquid:
Substratum: glucose 20.0g, casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, anhydrous sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, diammonium hydrogen citrate 2.0g, MgSO47H
2O 0.58g, MnSO
4H
2O 0.33g; Adding distil water is to 1000mL, 6.4,121 ℃ of sterilization 20min of pH.
Be inoculated into the lactobacillus rhamnosus bacterium liquid after activation in the triangular flask that contains above-mentioned substratum by 4% inoculum size, liquid amount is the bottled 200mL substratum of 500mL triangle, at 39 ℃, 100rpm shaking culture 48-72 hour, it is 10 that bacterium liquid centrifugal concentrating is adjusted bacterial concentration with stroke-physiological saline solution
10Cfu/mL.
1.2 subtilis bacterium liquid:
Substratum: extractum carnis 3g; Peptone 5g; Water 1000ml, 7.0,121 ℃ of sterilization 20min of pH;
Subtilis bacterium liquid after activation is inoculated into by 4% inoculum size in the triangular flask that contains above-mentioned substratum, and liquid amount is 500 mL, the bottled 200 mL substratum of triangle, at 37 ℃, 100rpm shaking culture 24-48 hour, with bacterium liquid centrifugal concentrating stroke-physiological saline solution, adjusting bacterial concentration is 10
10Cfu/mL.
1.3 outstanding fourth Pichia yeast liquid:
Substratum: yeast extract paste 10g; Peptone 20g, 10g glucose, pH 7.0,1000ml water, 121 ℃ of sterilization 20min.
Be inoculated into the outstanding fourth Pichia yeast liquid after activation in the triangular flask that contains above-mentioned substratum by 4% inoculum size, liquid amount is the bottled 200 mL substratum of 500 mL triangles, at 28-30 ℃, 200rpm shaking culture 48-72 hours, it is 10 that bacterium liquid centrifugal concentrating is adjusted bacterial concentration with stroke-physiological saline solution
10Cfu/mL.
1.4 outstanding fourth pichia spp is deposited separately; Lactobacillus rhamnosus mixes with subtilis; Standing for subsequent technique.
Two, zymotechnique: take pig blood as example
1, get 1000 kilograms of pig blood and be placed in sterilization tank;
2, get brown sugar 100-200kg, white sugar 100-200kg, add the slaughterhouse to clean drop blood groove organic waste water and make mixed solution 800-1500kg;
3, the syrup mixed solution is squeezed in sterilization tank, with the animal blood mix and blend, be warming up to 95 ± 5 ℃, sterilized 0.5-1 hour; Add successively when temperature rises to more than 85 ℃ and account for the oily angle stirring froth breaking that mixes rear material gross weight 0.5-1.0%;
4, be cooled to 50 ± 2 ℃, move to fermentor tank; In tank through 30 minutes, 115 ℃ of High Temperature Sterilizations;
5, add animal proteolytic enzyme 2.5-5.4kg, in tank, temperature is controlled at 50 ± 2 ℃; In present embodiment animal proteolytic enzyme used be Pangbo Bioengineering Co Ltd, Nanning produce " animal proteolytic enzyme A7, enzyme activity is: 150,000 u/g.
6, enzymolysis after 5 hours, is cooled to 38 ± 2 ℃, adds the outstanding fourth pichia spp of bacterial classification mixed solution 10kg(2kg, Bacillus licheniformis and 6kg, lactobacillus rhamnosus 2kg) stir, carry out aerobic, anaerobic stages fermentation.In the present embodiment, three kinds of bacterial classifications used all derive from China Committee for Culture Collection of Microorganisms agricultural microorganism center.
7, interim leavening temperature is connected with the oxygen supply time changing curve and is opened the filtration uncontaminated air pipeline valve that connects tank body, 38 ℃ ± 2 ℃ temperature continue enzymolysis below, and limit aerobic fermentation 10 hours is cooled to 30 ℃ ± 2 ℃, close and filter uncontaminated air valve, 15 hours anaerobically fermentings.
8, stirred 10 minutes every 2 hours during the aerobic fermentation enzymolysis.
9, through after 30 hours enzymatic hydrolysis and fermentations, sampling is surveyed more than viable count reaches 200,000,000/mL, surveys pH value 5-6, and Dan Lin Jia＞=4% can be cooled to 20 ℃ or room temperature, is filled in the container of specified volume to get final product.
Claims (9)
1. utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that described technique produces the liquid bio-organic fertilizer material with the method that animal proteolytic enzyme solution and fermented by mixed bacterium act on simultaneously take animal blood as fermentation substrate, and before implanting the beneficial microorganism bacterial classification, when heating up sterilization to raw and auxiliary material, with oily angle as defoamer, effective cancellation offscum, avoid material to overflow loss and contaminate environment, increase simultaneously organophosphorus content, in same still, first enzymolysis secondary fermentation; First aerobic rear anaerobism during fermentation; Stage realizes that enzymatic hydrolysis and fermentation synchronously carries out, and concrete steps are as follows:
A, get 1000 kilograms of animal bloods and be placed in sterilization tank;
B, get brown sugar 100-200kg, white sugar 100-200kg, add the slaughterhouse to clean drop blood groove organic waste water and make mixed solution 800-1500kg;
C, the syrup mixed solution is squeezed in sterilization tank, with the animal blood mix and blend, be warming up to 95 ± 5 ℃, sterilized 0.5-1 hour; Add successively when temperature rises to more than 85 ℃ and account for the oily angle stirring froth breaking that mixes rear material gross weight 0.5-1.0%;
D, be cooled to 50 ± 2 ℃, move to fermentor tank;
E, add proteolytic enzyme 2.5-5.4kg, in tank, temperature is controlled at 50 ± 2 ℃;
After f, enzymolysis 4-6 hour, be cooled to 38 ± 2 ℃, add bacterial classification mixed solution 10-15kg to stir, open the filtration uncontaminated air pipeline valve that connects tank body, at 38 ± 2 ℃ of temperature, enzymolysis was continued limit aerobic fermentation 10-12 hour in the limit, was cooled to 30 ± 2 ℃, close and filter uncontaminated air valve, 13-15 hour anaerobically fermenting;
G, sampling survey viable count are surveyed pH value 5-6 more than reaching 200,000,000/mL, and Dan Lin Jia＞=4% can be cooled to 20 ℃ or room temperature, carries out can.
2. according to claim 1ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that described animal blood is pig blood, ox blood, sheep blood or donkey blood.
3. according to claim 1ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that in described steps d, in the fermentor tank tank through 30 minutes, 115 ℃ of High Temperature Sterilizations.
4. according to claim 1ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that described proteolytic enzyme is animal proteolytic enzyme A7.
5. according to claim 1ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that stirring 10 minutes every 2 hours during described aerobic fermentation enzymolysis.
6. according to claim 1ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, it is characterized in that described mixed bacteria liquid is the mixture of outstanding fourth pichia spp, Bacillus licheniformis and lactobacillus rhamnosus, the mass ratio of outstanding fourth pichia spp, Bacillus licheniformis and lactobacillus rhamnosus is 2-5:5-10:2-5.
7. according to claim 6ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, the bacterial concentration that it is characterized in that described lactobacillus rhamnosus is 10
10Cfu/mL.
8. according to claim 6ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, the bacterial concentration that it is characterized in that described outstanding fourth pichia spp is 10
10Cfu/mL.
9. according to claim 6ly utilize the animal blood enzymatic hydrolysis and fermentation and prepare liquid fertilizer technique take oily angle as defoamer, the bacterial concentration that it is characterized in that described Bacillus licheniformis is 10
10Cfu/mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105461385A (en) * | 2015-11-09 | 2016-04-06 | 新疆农业大学 | Water-soluble amino acid fertilizer and preparation method thereof |
| TWI640616B (en) * | 2018-03-05 | 2018-11-11 | 國立高雄科技大學 | Method for treating petroleum-contaminated soil by fermented blood |
| CN110651888A (en) * | 2019-04-12 | 2020-01-07 | 株式会社三多 | Method for producing fermented product of pig blood, and antibacterial composition and chicken feed composition using the fermented product |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101525253A (en) * | 2008-03-03 | 2009-09-09 | 迈德金生物技术（北京）有限公司 | Microbial fertilizer and preparation method thereof |
| CN101913922A (en) * | 2010-08-11 | 2010-12-15 | 牟钰德 | Technology for preparing liquid bio-organic fertilizer by using animal blood fermentation method |
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2013
- 2013-04-01 CN CN201310108660.7A patent/CN103145459B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101525253A (en) * | 2008-03-03 | 2009-09-09 | 迈德金生物技术（北京）有限公司 | Microbial fertilizer and preparation method thereof |
| CN101913922A (en) * | 2010-08-11 | 2010-12-15 | 牟钰德 | Technology for preparing liquid bio-organic fertilizer by using animal blood fermentation method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105461385A (en) * | 2015-11-09 | 2016-04-06 | 新疆农业大学 | Water-soluble amino acid fertilizer and preparation method thereof |
| TWI640616B (en) * | 2018-03-05 | 2018-11-11 | 國立高雄科技大學 | Method for treating petroleum-contaminated soil by fermented blood |
| CN110651888A (en) * | 2019-04-12 | 2020-01-07 | 株式会社三多 | Method for producing fermented product of pig blood, and antibacterial composition and chicken feed composition using the fermented product |
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