CN103141659A - Chewing gum containing sweet tea extract and preparation method thereof - Google Patents
Chewing gum containing sweet tea extract and preparation method thereof Download PDFInfo
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Abstract
The invention provides a chewing gum containing a sweet tea extract and a preparation method thereof. The sweet ingredient is a sweet tea extract which accounts for 0.2-10 percent of the weight of the chewing gum; the rubusoside weight accounts for 5-40 percent of the sweet tea extract; and the sweet tea polyphenol accounts for 2-21 percent. The preparation method comprises the following steps: mixing the sweet tea extract and a gum base, heating the mixture at the temperature of 50-60 DEG C until the extract is melted, uniformly stirring, and cooling to form a sugar blank; rolling the sugar blank into sugar tablets, cooling, and slicing so as to obtain the chewing gum. The chewing gum takes the sweet tea extract as the sweet ingredient, when the rubusoside in the sweet tea extract accounts for 5-40 percent and the content of the sweet tea polyphenol ranges from 2 to 21 percent, the rubusoside and the sweet tea polyphenol interact with each other, and the effects of preventing decayed tooth and improving the mouth health can be achieved. When the sweet tea extract in the chewing gum has a certain content, the effect of preventing decayed tooth is improved along with decrease of the rubusoside content and the increase of the sweet tea polyphenol content.
Description
Technical field
The present invention relates to a kind of chewing gum, be specifically related to a kind of chewing gum that contains sweet tea extract and preparation method thereof.
Background technology
Sweet tea (Rubus Suavissmus S Lee) is the distinctive natural sweet taste plant in Guangxi, is also best in the world Gao Tian, low-heat, safety non-toxic and the sweet taste plant with health care.Approximately contain in sweet tea more than 3.2% flavone compound, the sweet tea tannins of 9% left and right and 5～8% glycosides (Rubusoside), in addition, also be rich in the necessary trace element of several amino acids and human body, have higher nutritive value and health care value.Glycosides is the sweet taste factor that sweet tea presents the sweet taste mouthfeel, and it is the low in calories high sweet substance of the diterpene glucoside that is combined into of steviol (Steviol) and glucose, and its molecular formula is C
32H
50O
12The glycosides that extracts from sweet tea is mouthfeel the best in the sweet taste plant, and the pure degree of sweet taste is near white sugar, and its sugariness is equivalent to 300 times of sucrose, is sugared Dai Pin best in the Nature.Glycosides can also suppress in the oral cavity to produce the activity of the glucosyltransferase (GTF) of cariogenic streptococcus mutans (S.mutans) growth and synthetic glucan, thereby suppress the water-insoluble glucan (WIG) of strong adhesion and the generation of acid, prevent the generation of bacterial plaque, reach the effect of caries prevention.Sweet tea tannins is active component important in sweet tea, comprise distinctive GOD type Ellagitannins, also comprise gallic acid, ellagic acid, 2-pyrones-4, the 6-dicarboxylic acids, brevifolin carboxylic acid, sanguisorbic acid dilactone, caffeic acid etc., having obviously anti-oxidant, anti-sudden change, reduce cholesterol, reduce low-density lipoprotein in blood, suppress increased blood pressure, suppress blood sugar and rise, suppress platelet aggregation, antibiotic, anti-food hypersenstivity, improve the effect of stomach microbial environment, smelly eliminating, is to find at present optimal natural antioxidant.Therefore, sweet tea extract is a kind of desirable sweetener, can be applicable to the chewing gum production field.
the document " comparative study to the insoluble exocellular polysaccharide of streptococcus mutans synthetic water of glycosides and xylitol " that is published in the 32nd the 3rd phase of volume of " Guangxi medical science " March in 2010 is take glycosides as raw material, with the tryptone-tryptose that contains 0.5% sucrose-yeast extract medium (TTY), adopt doubling dilution to be made into the glycosides solution of 5 kinds of variable concentrations, be followed successively by 5g/100ml, 2.5g/100ml, 0.125g/100ml, 0.625g/100ml, 0.313g/100ml, the glycosides solution of above-mentioned concentration has all produced certain inhibitory action to the insoluble exocellular polysaccharide of streptococcus mutans synthetic water, in above-mentioned concentration range, reduction along with glycosides concentration, inhibition descends.the document " sweet tea protects gear piece to the impact of glucosyltransferase and extracellular water-insoluble polysaccharide " that is published in the 34th the 6th phase of volume of " Guangxi medical science " June in 2012 is to integrate with Guilin the sweet tea that fine jade strength natural Co., Ltd produces to protect the tooth lozenge as raw material, with the tryptone-tryptose that contains 1% sucrose-yeast extract medium (TTY), adopt doubling dilution to be made into the solution of 5 kinds of variable concentrations, be followed successively by 5g/50ml, 2.5g/50ml, 1.250g/50ml, 0.625g/50ml, 0.313g/50ml, the content that protects glycosides in the tooth lozenge due to this sweet tea is 5.5%, be converted into the concentration of glycosides, be followed successively by 0.275g/50ml, 0.138g/50ml, 0.068g/50ml, 0.034g/50ml, 0.017g/50ml, the sweet tea of above-mentioned concentration protects the tooth lozenge has obvious inhibitory action to streptococcus mutans GTF enzyme activity, also can suppress the synthetic of WIG, in above-mentioned concentration range, reduction along with glycosides concentration, inhibition descends.Comprehensive above-mentioned two pieces of documents, we can draw a conclusion, and namely glycosides concentration is higher, and is stronger to the inhibition of streptococcus mutans, otherwise, more weak.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of chewing gum that contains sweet tea extract and preparation method thereof, this chewing gum is take sweet tea extract as sweet ingredient, when the sweet tea tannins that contains in sweet tea extract than the glycosides of low dosage and higher dosage, the effect that can reach caries prevention preferably and improve oral health.
Technical scheme provided by the invention is a kind of chewing gum that contains sweet tea extract, comprises matrix and sweet ingredient, and described sweet ingredient is sweet tea extract, and it accounts for 0.2～10% of this chewing gum weight; In described sweet tea extract, the weight content of glycosides is 5～40%, and the weight content of sweet tea tannins is 2～21%.
Sweet tea extract of the present invention accounts for 0.2～5% of this chewing gum weight.
Sweet tea extract of the present invention accounts for 0.2～3.5% of this chewing gum weight.
Sweet tea extract of the present invention accounts for 0.2～2.5% of this chewing gum weight.
Sweet tea extract of the present invention accounts for 2.5% of this chewing gum weight.
In sweet tea extract of the present invention, the weight content of glycosides is 5～40%, and the weight content of sweet tea tannins is 7～21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5～20%, and the weight content of sweet tea tannins is 17～21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5～10%, and the weight content of sweet tea tannins is 18～21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5%, and the weight content of sweet tea tannins is 21%.
For improving mouthfeel, also can add the tart flavour conditioning agent in chewing gum of the present invention, it accounts for 0.2～1.2% of this chewing gum weight.The tart flavour conditioning agent can be selected a kind of in citric acid, tartaric acid and malic acid.
For improving mouthfeel, fresh breath also can add peppermint oil in chewing gum of the present invention, and it accounts for 0.05%～0.5% of this chewing gum weight.
The present invention also provides the above-mentioned preparation method who contains the chewing gum of sweet tea extract, and sweet tea extract and matrix are mixed, and is heated to its melting under 50～60 ℃, stirs evenly the cooling candy batch that is; Candy batch is rolled into sugar-tablet, cooling, stripping and slicing, and get final product.
In above-mentioned mixed process, can also add the tart flavour conditioning agent that accounts for this chewing gum weight 0.2～1.2%, described tart flavour conditioning agent is citric acid, tartaric acid or malic acid.
In above-mentioned mixed process, can also add the peppermint oil that accounts for this chewing gum weight 0.05%～0.5%.
The sweet ingredient that contains the chewing gum of sweet tea extract of the present invention is sweet tea extract, when the content of glycosides in sweet tea extract 5～40% and the scope of sweet tea tannins 2～21% in, glycosides and sweet tea tannins interact, the activity that can suppress preferably the Streptococcus mutans growth and synthesize the glucosyltransferase (GTF) of glucan, thereby suppress the water-insoluble glucan (WIG) of strong adhesion and the generation of acid, prevent the generation of bacterial plaque, and then reach the effect of caries prevention.In chewing gum of the present invention sweet tea extract content one regularly, its caries prevention effect reduces along with the rising of glycosides content in sweet tea extract, reducing of sweet tea tannins content.
The specific embodiment
The present invention is further elaborated for following specific embodiment, but not as a limitation of the invention.
Following percentage is percetage by weight.Embodiment 1
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 21% of sweet tea extract weight.
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, be 40 ℃ of lower refluxing extraction 2 times in ultrasonic wave 40KHz, temperature, extract 20min at every turn, merge extract, filter, get filtrate and reclaim solvent, get sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 9, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 70%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 21%.
Embodiment 2
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 0.2%, matrix 99.8%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 ℃ of water lixiviates 2 times, each lixiviate 20min merges extract, filters, and concentrated, drying is sweet tea extract, and wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 2%.
Embodiment 3
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 10%(v/v) ethanol, normal temperature lixiviate 2 times, each lixiviate 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) with the D101 macroporous absorbent resin, sweet tea crude extract is carried out adsorbing separation, uses 50%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, and wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 21%.
Embodiment 4
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 30%(v/v) ethanol, normal temperature lixiviate 2 times, each lixiviate 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) with the D101 macroporous absorbent resin, sweet tea crude extract is carried out adsorbing separation, uses 50%(v/v) ethanol elution, eluent is concentrated, and drying is sweet tea extract, and wherein, the weight content of glycosides is 10%, the weight content of sweet tea tannins is 18%.
Embodiment 5
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 15% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, be 40 ℃ of lower lixiviates 2 times in ultrasonic wave 40KHz, temperature, each lixiviate 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 11, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 70%(v/v) ethanol elution, eluent is concentrated, and drying is sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 15%.
Reference examples 1
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 70%, sweet tea tannins accounts for 7% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water and mix, add the cellulase of sweet tea weight 0.3%, enzymolysis is 2 hours under 40 ℃ of temperature, pH4.5 condition, and the enzyme that goes out filters, and gets filtrate;
2) the macromolecular filtering film that is 10,000 molecular weight with filtrate by the aperture separates, and obtains parting liquid;
3) parting liquid is carried out adsorbing separation with anion exchange resin, obtain parting liquid for the second time, parting liquid carries out adsorbing separation by ADS-7 type macroporous absorbent resin for the second time again, then use 50%(v/v) ethanol elution, get eluent concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 70%, the weight content of sweet tea tannins is 7%.
Reference examples 2
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 95%, sweet tea tannins accounts for 2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water and mix, add the cellulase of sweet tea weight 0.5%, the complex enzyme of hemicellulase, enzymolysis is 3 hours under 40 ℃ of temperature, pH4.5 condition, and the enzyme that goes out filters, and gets filtrate;
2) be that the hollow ceramic film device that can hold back 10,000 molecular weight carries out ultra-filtration and separation with filtrate by the aperture, get trapped fluid and parting liquid;
3) parting liquid is carried out adsorbing separation by anion exchange resin, obtain parting liquid for the second time, parting liquid carries out adsorbing separation by ADS-7 type macroporous absorbent resin for the second time again, then use 70%(v/v) ethanol elution, eluent is concentrated, and drying is sweet tea extract, wherein, the weight content of glycosides is 95%, the weight content of sweet tea tannins is 2%.
Embodiment 6
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 15% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 5.
Embodiment 7
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 8
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 35%, sweet tea tannins accounts for 16% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, be 40 ℃ of lower lixiviates 2 times in ultrasonic wave 30KHz, temperature, each 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 10.5, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 60%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, wherein, the weight content of glycosides is 35%, the weight content of sweet tea tannins is 16%.
Embodiment 9
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 96.5%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 4.
Embodiment 10
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 96.5%; Wherein, glycosides account for sweet tea extract weight 20%, sweet tea tannins accounts for 20% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 30%(v/v) ethanol, be lixiviate 20min under 40 ℃ of conditions in temperature, filter, get filtrate and reclaim solvent, get sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 10, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 50%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, wherein, the weight content of glycosides is 20%, the weight content of sweet tea tannins is 20%.
Embodiment 11
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 1.5%, matrix 98.5%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 4.
Embodiment 12
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 1.5%, matrix 98.5%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 13
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 0.3%, matrix 99.7%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 14
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 0.5%, matrix 99.5%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 15
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, tartaric acid 1%; Wherein, glycosides account for sweet tea extract weight 30%, sweet tea tannins accounts for 19% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 10%(v/v) ethanol, be lixiviate 2 times under 30 ℃ of conditions in ultrasonic wave 20KHz, temperature, each 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 10.8, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 50%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, wherein, the weight content of glycosides is 30%, the weight content of sweet tea tannins is 19%.
Embodiment 16
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 95.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 8.9%, sweet tea tannins accounts for 20.2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, normal temperature lixiviate 2 times, each 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 9.5, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 60%(v/v) ethanol elution, get eluent concentrated, drying is sweet tea extract, wherein, the weight content of glycosides is 8.9%, the weight content of sweet tea tannins is 20.2%.
Embodiment 17
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 5% of sweet tea extract weight.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 ℃ of water lixiviate 30min, merge extract, filter, concentrated, drying is sweet tea extract, and wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 5%.
Embodiment 18
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 8%, matrix 91%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 7% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, be 70 ℃ of lower lixiviates 2 times in ultrasonic wave 40KHz, temperature, each lixiviate 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 12.5, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 50%(v/v) ethanol elution, eluent is concentrated, and drying is sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 7%.
Embodiment 19
The formula that contains the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 10% of sweet tea extract weight.
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, be 60 ℃ of lower lixiviates 2 times in ultrasonic wave 40KHz, temperature, each lixiviate 20min merges extract, filters, and gets filtrate and reclaims solvent, gets sweet tea crude extract.
2) adding limewash to make its pH value in sweet tea crude extract is 11, and after contamination precipitation, the elimination precipitation is got supernatant standby.
3) regulate supernatant pH to neutrality, with the D101 macroporous absorbent resin, it carried out adsorbing separation, use 50%(v/v) ethanol elution, eluent is concentrated, and drying is sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 10%.
Embodiment 1～13 and reference examples 1, the 2 described preparation methods that contain the chewing gum of sweet tea extract mix sweet tea extract and matrix, are heated to its melting under 50～60 ℃, stir evenly the cooling candy batch that is; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing is sweet tea chewing gum.
The described preparation method who contains the chewing gum of sweet tea extract of embodiment 15 mixes sweet tea extract, matrix and tartaric acid, is heated to its melting under 50～60 ℃, stirs evenly the cooling candy batch that is; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing is sweet tea chewing gum.
The described preparation method who contains the chewing gum of sweet tea extract of embodiment 16～19 mixes sweet tea extract, matrix, malic acid and peppermint oil, is heated to its melting under 50～60 ℃, stirs evenly the cooling candy batch that is; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing is sweet tea chewing gum.
Experimental example
For showing the chewing gum of sweet tea extract that contains of the present invention to the preventive effect of carious tooth, the applicant has carried out experiment in vitro research for this chewing gum to the mechanism of action of cariogenic bacteria (streptococcus mutans), specifically is reported as follows:
1, experimental technique
1.1 the preparation of chewing gum solution and bacteria suspension
Experimental group: the chewing gum 25g that gets respectively the embodiment of the present invention 3～5 and reference examples 1～2, with its pulverizing, be placed in test tube, then add respectively the TTY fluid nutrient medium (tryptone-tryptose-yeast extract medium) that 100ml contains 1% sucrose and be made into chewing gum solution.
Negative control group: adopt the TTY fluid nutrient medium that does not contain this chewing gum.
With streptococcus mutans (S.mutans) after TSB culture medium (Trypsin Induced soybean fluid nutrient medium) activation 3h, being inoculated in 37 ℃ of lower anaerobism of MSB solid medium (light saliva is selected culture medium) cultivated 48 hours, the picking colonies typical is made suspension in the 5ml SPSS, harmonic length is the 540nm place, OD value (Optical Density, OD) is 1.0 bacteria suspension.
1.2, chewing gum solution is to the cariogenic bacteria affects on the growth
Get respectively the 0.3ml bacterial suspension inoculation in the chewing gum solution of 3ml variable concentrations, 37 ℃ of lower anaerobism were cultivated 48 hours, and each test tube bacterium of the centrifugal collection of difference, were placed in 2ml physiological saline, mixing, it is the OD value at 540nm place that ultra-violet and visible spectrophotometer is surveyed wavelength.
1.3, chewing gum solution is on the impact of S.mutans acid production
Get respectively the 3ml bacterial suspension inoculation in the chewing gum solution of each concentration of equivalent, 37 ℃ of lower anaerobism were cultivated 48 hours, and calculated the △ pH value before and after cultivating, and contrasted with the △ pH value of negative control group.
1.4, chewing gum solution is on the impact of S.mutans adhesive attraction
Be the ratio of 10:1 in chewing gum solution and bacteria suspension, respectively with bacterial suspension inoculation in the chewing gum solution of each concentration, after 30 ° of horizontal angle anaerobism of all test tubes are cultivated 48h, with the bacterium that adheres to test tube wall NaOH eluant solution, measure wavelength in the OD value at 540nm place, and calculate the adhesion inhibition rate, with the adhesion inhibition rate contrast of negative control group.
1.5, the extraction of glucosyltransferase (Glucosyltransferase, GTF) and the detection of water-insoluble exocellular polysaccharide (WIG)
Get each 3ml of chewing gum solution of variable concentrations, add S.mutans bacteria suspension 0.3ml, anaerobism is centrifugal with inoculum after cultivating, collect supernatant, add solid ammonium sulfate, make saturation degree reach 60%, after centrifugal, precipitation is dissolved in phosphate buffer dialyses, centrifugal, supernatant is the thick enzyme of GTF.Get the thick enzyme of 0.1mlGTF, add 4ml distilled water, adopt Coomassie brilliant blue method (Bradford method) to measure total protein content.Separately get 100 μ l0.5mmol/L sucrose solutions, the thick enzyme 0.1ml of GTF, add the 0.1mmol/L sodium phosphate buffer, make the cumulative volume of mixed liquor reach 500 μ l, hatch 60min for 37 ℃, be heated to cessation reaction after 100 ℃ of lasting 10min, add 2.5ml distilled water mixing, centrifugal, collect supernatant and adopt the Smogyi method to measure reduced sugar amount in enzyme one substrate reactions liquid.GTF unit of activity (IU) is defined as (37 ℃ of reaction 1h are per minute from the sucrose release 1 required enzyme amount of μ mol reduced sugar) under standard conditions, and calculates the ratio vigor of enzyme.
Get inoculum centrifugal, abandon supernatant, taking precipitate adds the NaOH5ml of 0.5mol/L, centrifugal, get supernatant, add the absolute ethyl alcohol of its 3 times of volumes, centrifugal, precipitation is the water-insoluble exocellular polysaccharide that reaction generates, and measures the content of water-insoluble exocellular polysaccharide with anthrone method.Enzymatic activity and the contrast of insoluble exocellular polysaccharide with S.mutans in negative control group.
2, experimental result:
2.1, in above-mentioned experiment, streptococcus mutans growth is all arranged in chewing gum solution, and along with the rising of glycosides content in sweet tea extract and the reduction of sweet tea tannins content, in solution, the concentration of streptococcus mutans increases.The OD value population mean of experimental group S.mutans is lower than negative control group (P＜0.01), and its inhibition increases with the reduction of glycosides content in sweet tea extract and the increase of sweet tea tannins.Concrete outcome sees Table 1.
Table 1 chewing gum solution is to the inhibiting OD value of streptococcus mutans (X ± s)
2.2, all have difference between △ pH (pH variable quantity) population mean before and after the chewing gum solution experiments, in sweet tea extract glycosides content be 5%, when sweet tea tannins content is 21%, the △ pH value variable quantity of S.mutans is minimum; Between the population mean of experimental group △ pH value significantly lower than negative control group △ pH (P＜0.001).Specifically see Table 2.
Table 2 chewing gum solution produces effect of acid (X ± s) to streptococcus mutans
2.3, along with the rising of glycosides content in sweet tea extract, the reduction of sweet tea tannins content, the adhesion inhibition rate also decreases.The population mean of the OD value of experimental group adhesion bacterium liquid is significantly lower than negative control group group (p＜0.01), and concrete outcome sees Table 3.
Table 3 chewing gum solution is on the impact of Adherence of Streptococcus mutans (X ± s)
2.4, experimental group is on the impact of total protein, GTF enzyme activity, water-insoluble exocellular polysaccharide in S.mutans enzyme liquid
2.4.1 along with glycosides content in sweet tea extract raises, the reduction of sweet tea tannins content, in S.mutans enzyme liquid, the content of total protein reduces on the contrary.In the chewing gum solution of experimental group variable concentrations, total protein content is on the contrary higher than the negative control group group.Specifically see Table 4.
Table 4 chewing gum solution is on the impact of total protein content in S.mutans enzyme liquid (X ± s)
2.4.2 along with glycosides content in sweet tea extract raises, the reduction of sweet tea tannins content, the GTF activity of S.mutans raises gradually.The reduced sugar amount of negative control group, enzyme activity, than vigor all apparently higher than experimental group (p＜0.001).Specifically see Table 5.
Table 5 chewing gum solution is on the impact of GTF in S.mutans (X ± s)
2.4.3 along with glycosides content in sweet tea extract raises, the reduction of sweet tea tannins content, the synthetic water-insoluble exocellular polysaccharide content of S.mutans is rising trend; The ability of the insoluble exocellular polysaccharide of negative control group S.mutans synthetic water is significantly higher than experimental group (p＜0.001).Specifically see Table 6.
Table 6 chewing gum solution is on the impact of the insoluble exocellular polysaccharide of S.mutans synthetic water (X ± s)
Through above-mentioned experiment, in the GTF enzymatic activity of experimental group and sweet tea extract, glycosides content is proportionate, be negative correlativing relation with sweet tea tannins content, in chewing gum the content one of sweet tea extract regularly, to the inhibition of S.mutans along with glycosides content raises, sweet tea tannins content reduces and reduces.
Claims (10)
1. the chewing gum that contains sweet tea extract comprises matrix and sweet ingredient, it is characterized in that: described sweet ingredient is sweet tea extract, and it accounts for 0.2～10% of this chewing gum weight; In described sweet tea extract, the weight content of glycosides is 5～40%, and the weight content of sweet tea tannins is 2～21%.
2. the chewing gum that contains sweet tea extract according to claim 1, it is characterized in that: described sweet tea extract accounts for 0.2～5% of this chewing gum weight.
3. the chewing gum that contains sweet tea extract according to claim 2, it is characterized in that: described sweet tea extract accounts for 0.2～3.5% of this chewing gum weight.
4. the chewing gum that contains sweet tea extract according to claim 3, it is characterized in that: described sweet tea extract accounts for 0.2～2.5% of this chewing gum weight.
5. the chewing gum that contains sweet tea extract according to claim 4, it is characterized in that: described sweet tea extract accounts for 2.5% of this chewing gum weight.
6. the described chewing gum that contains sweet tea extract of any one according to claim 1～5, it is characterized in that: in described sweet tea extract, the weight content of glycosides is 5～40%, the weight content of sweet tea tannins is 7～21%.
7. the described chewing gum that contains sweet tea extract of any one according to claim 1～5, it is characterized in that: in described sweet tea extract, the weight content of glycosides is 5～20%, the weight content of sweet tea tannins is 17～21%.
8. the described chewing gum that contains sweet tea extract of any one according to claim 1～5, it is characterized in that: in described sweet tea extract, the weight content of glycosides is 5～10%, the weight content of sweet tea tannins is 18～21%.
9. the described chewing gum that contains sweet tea extract of any one according to claim 1～5, it is characterized in that: in described sweet tea extract, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 21%.
10. the preparation method who contains the chewing gum of sweet tea extract claimed in claim 1, is characterized in that: sweet tea extract and matrix are mixed, be heated to its melting under 50～60 ℃, stir evenly the cooling candy batch that is; Candy batch is rolled into sugar-tablet, cooling, stripping and slicing, and get final product.
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| CN114223774A (en) * | 2021-12-24 | 2022-03-25 | 中国农业科学院都市农业研究所 | Sugar-free chewing gum containing Lithocarpus litseifolius extract and preparation method thereof |
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