CN103141659B - Chewing gum containing sweet tea extract and preparation method thereof - Google Patents
Chewing gum containing sweet tea extract and preparation method thereof Download PDFInfo
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Abstract
The invention provides a chewing gum containing a sweet tea extract and a preparation method thereof. The sweet ingredient is a sweet tea extract which accounts for 0.2-10 percent of the weight of the chewing gum; the rubusoside weight accounts for 5-40 percent of the sweet tea extract; and the sweet tea polyphenol accounts for 2-21 percent. The preparation method comprises the following steps: mixing the sweet tea extract and a gum base, heating the mixture at the temperature of 50-60 DEG C until the extract is melted, uniformly stirring, and cooling to form a sugar blank; rolling the sugar blank into sugar tablets, cooling, and slicing so as to obtain the chewing gum. The chewing gum takes the sweet tea extract as the sweet ingredient, when the rubusoside in the sweet tea extract accounts for 5-40 percent and the content of the sweet tea polyphenol ranges from 2 to 21 percent, the rubusoside and the sweet tea polyphenol interact with each other, and the effects of preventing decayed tooth and improving the mouth health can be achieved. When the sweet tea extract in the chewing gum has a certain content, the effect of preventing decayed tooth is improved along with decrease of the rubusoside content and the increase of the sweet tea polyphenol content.
Description
Technical field
The present invention relates to a kind of chewing gum, be specifically related to a kind of chewing gum containing sweet tea extract and preparation method thereof.
Background technology
Sweet tea (Rubus Suavissmus S Lee) is the distinctive natural sweet taste plant in Guangxi, is also Gao Tian best in the world, low-heat, safety non-toxic and have the sweet-tasting plant of health care.In sweet tea about containing more than 3.2% flavone compound, the sweet tea tannins of about 9% and the glycosides (Rubusoside) of 5 ~ 8%, in addition, also be rich in several amino acids and the necessary trace element of human body, there is higher nutritive value and health value.Glycosides is the sweet taste factor that sweet tea presents sweet taste mouthfeel, and it is the low in calories high sweet substance of the diterpene glucoside that steviol (Steviol) and glucose are combined into, and its molecular formula is C
32h
50o
12.The glycosides extracted from sweet tea is mouthfeel the best in sweet-tasting plant, and the pure degree of sweet taste is close to white sugar, and its sugariness is equivalent to 300 times of sucrose, is sugared Dai Pin best in the Nature.Glycosides can also suppress the activity of the glucosyltransferase (GTF) producing cariogenic streptococcus mutans (S.mutans) growth and synthesis glucan in oral cavity, thus suppress the water-insoluble glucan (WIG) of strong adhesion and the generation of acid, prevent the generation of bacterial plaque, reach the effect of caries prevention.Sweet tea tannins is active component important in sweet tea, comprise distinctive GOD type Ellagitannins, also comprise gallic acid, ellagic acid, 2-pyrones-4,6-dicarboxylic acids, brevifolin carboxylic acid, sanguisorbic acid dilactone, caffeic acid etc., the effect having obviously anti-oxidant, anti-sudden change, reduce cholesterol, reduce low-density lipoprotein in blood, suppress blood pressure to rise, suppress blood sugar to rise, suppress platelet aggregation, antibacterial, anti-food hypersenstivity, improve gastrointestinal microbiological environment, smelly eliminating finds optimal natural antioxidant at present.Therefore, sweet tea extract is a kind of desirable sweetener, can be applicable to chewing gum production field.
The document " glycosides and xylitol are to the comparative study of the insoluble exocellular polysaccharide of streptococcus mutans synthetic water " being published in volume the 3rd phase in " Guangxi medical science " March the 32nd in 2010 take glycosides as raw material, by the tryptone-tryptose-yeast extract medium (TTY) containing 0.5% sucrose, doubling dilution is adopted to be made into the glycosides solution of 5 kinds of variable concentrations, be followed successively by 5g/100ml, 2.5g/100ml, 0.125g/100ml, 0.625g/100ml, 0.313g/100ml, the glycosides solution of above-mentioned concentration all creates certain inhibitory action to the insoluble exocellular polysaccharide of streptococcus mutans synthetic water, in above-mentioned concentration range, along with the reduction of glycosides concentration, inhibition declines.The document " sweet tea protects the impact of gear piece on glucosyltransferase and extracellular water-insoluble polysaccharide " being published in volume the 6th phase in " Guangxi medical science " June the 34th in 2012 integrates Sweet-tea tooth protection lozenge that the natural Co., Ltd of fine jade strength produces as raw material with Guilin, by the tryptone-tryptose-yeast extract medium (TTY) containing 1% sucrose, doubling dilution is adopted to be made into the solution of 5 kinds of variable concentrations, be followed successively by 5g/50ml, 2.5g/50ml, 1.250g/50ml, 0.625g/50ml, 0.313g/50ml, because in this Sweet-tea tooth protection lozenge, the content of glycosides is 5.5%, be converted into the concentration of glycosides, be followed successively by 0.275g/50ml, 0.138g/50ml, 0.068g/50ml, 0.034g/50ml, 0.017g/50ml, the Sweet-tea tooth protection lozenge of above-mentioned concentration has obvious inhibitory action to streptococcus mutans GTF enzyme activity, also the synthesis of WIG can be suppressed, in above-mentioned concentration range, along with the reduction of glycosides concentration, inhibition declines.Comprehensively above-mentioned two sections of documents, we can draw a conclusion, and namely glycosides concentration is higher, stronger to the inhibition of streptococcus mutans, otherwise, then more weak.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of chewing gum containing sweet tea extract and preparation method thereof, this chewing gum take sweet tea extract as sweet ingredient, when in sweet tea extract containing sweet tea tannins compared with the glycosides of low dosage and higher dosage time, good caries prevention can be reached and improve the effect of oral health.
Technical scheme provided by the invention is a kind of chewing gum containing sweet tea extract, and comprise matrix and sweet ingredient, described sweet ingredient is sweet tea extract, and it accounts for 0.2 ~ 10% of this gum; In described sweet tea extract, the weight content of glycosides is 5 ~ 40%, and the weight content of sweet tea tannins is 2 ~ 21%.
Sweet tea extract of the present invention accounts for 0.2 ~ 5% of this gum.
Sweet tea extract of the present invention accounts for 0.2 ~ 3.5% of this gum.
Sweet tea extract of the present invention accounts for 0.2 ~ 2.5% of this gum.
Sweet tea extract of the present invention accounts for 2.5% of this gum.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 40%, and the weight content of sweet tea tannins is 7 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 20%, and the weight content of sweet tea tannins is 17 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 10%, and the weight content of sweet tea tannins is 18 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5%, and the weight content of sweet tea tannins is 21%.
For improving mouthfeel, also can add acidity regulator in chewing gum of the present invention, it accounts for 0.2 ~ 1.2% of this gum.Acidity regulator can select the one in citric acid, tartaric acid and malic acid.
For improving mouthfeel, fresh breath, also can add peppermint oil in chewing gum of the present invention, it accounts for 0.05% ~ 0.5% of this gum.
Present invention also offers the preparation method of the above-mentioned chewing gum containing sweet tea extract, by sweet tea extract and matrix mixing, at 50 ~ 60 DEG C, be heated to its melting, stir evenly, cooling is candy batch; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing, to obtain final product.
In above-mentioned mixed process, can also add the acidity regulator accounting for this gum 0.2 ~ 1.2%, described acidity regulator is citric acid, tartaric acid or malic acid.
In above-mentioned mixed process, the peppermint oil accounting for this gum 0.05% ~ 0.5% can also be added.
The sweet ingredient of the chewing gum containing sweet tea extract of the present invention is sweet tea extract, when glycosides in sweet tea extract content 5 ~ 40% and sweet tea tannins in the scope of 2 ~ 21%, glycosides and sweet tea tannins interact, the activity of the glucosyltransferase (GTF) of Streptococcus mutans growth and synthesis glucan can be suppressed preferably, thus suppress the water-insoluble glucan (WIG) of strong adhesion and the generation of acid, prevent the generation of bacterial plaque, and then reach the effect of caries prevention.Sweet tea extract content one timing in chewing gum of the present invention, its caries prevention effect is along with the rising of glycosides content in sweet tea extract, the reduction of sweet tea tannins content and reducing.
Detailed description of the invention
The present invention is further elaborated for following specific embodiment, but not as a limitation of the invention.
Following percentage is percetage by weight.Embodiment 1
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 21% of sweet tea extract weight.
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, at ultrasonic wave 40KHz, temperature are 40 DEG C, refluxing extraction 2 times, extracts 20min at every turn, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 9, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 70%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 21%.
Embodiment 2
Formula containing the chewing gum of sweet tea extract: sweet tea extract 0.2%, matrix 99.8%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 DEG C of water lixiviates 2 times, each lixiviate 20min, merge extract, filter, concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 2%.
Embodiment 3
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 10%(v/v) ethanol, normal temperature lixiviate 2 times, each lixiviate 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) with D101 macroporous absorbent resin, adsorbing separation is carried out, with 50%(v/v to sweet tea crude extract) ethanol elution, get eluent and concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 21%.
Embodiment 4
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 30%(v/v) ethanol, normal temperature lixiviate 2 times, each lixiviate 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) with D101 macroporous absorbent resin, adsorbing separation is carried out, with 50%(v/v to sweet tea crude extract) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 10%, the weight content of sweet tea tannins is 18%.
Embodiment 5
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 15% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasonic wave 40KHz, temperature are 40 DEG C, each lixiviate 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 11, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 70%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 15%.
Reference examples 1
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 70%, sweet tea tannins accounts for 7% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water mixing, adds the cellulase of sweet tea weight 0.3%, enzymolysis 2 hours under temperature 40 DEG C, pH4.5 condition, and go out enzyme, filters, obtain filtrate;
2) filtrate is separated by the macromolecular filtering film that aperture is 10,000 molecular weight, obtains parting liquid;
3) parting liquid anion exchange resin is carried out adsorbing separation, obtain second time parting liquid, again second time parting liquid is carried out adsorbing separation by ADS-7 type macroporous absorbent resin, then use 50%(v/v) ethanol elution, get eluent concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 70%, the weight content of sweet tea tannins is 7%.
Reference examples 2
Formula containing the chewing gum of sweet tea extract: sweet tea extract 10%, matrix 90%; Wherein, glycosides account for sweet tea extract weight 95%, sweet tea tannins accounts for 2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water mixing, adds the cellulase of sweet tea weight 0.5%, the complex enzyme of hemicellulase, enzymolysis 3 hours under temperature 40 DEG C, pH4.5 condition, and go out enzyme, filters, obtain filtrate;
2) be that the hollow ceramic film device that can retain 10,000 molecular weight carries out ultra-filtration and separation by filtrate by aperture, obtain trapped fluid and parting liquid;
3) parting liquid is carried out adsorbing separation by anion exchange resin, obtain second time parting liquid, again second time parting liquid is carried out adsorbing separation by ADS-7 type macroporous absorbent resin, then use 70%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 95%, the weight content of sweet tea tannins is 2%.
Embodiment 6
Formula containing the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 15% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 5.
Embodiment 7
Formula containing the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 8
Formula containing the chewing gum of sweet tea extract: sweet tea extract 5%, matrix 95%; Wherein, glycosides account for sweet tea extract weight 35%, sweet tea tannins accounts for 16% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, lixiviate 2 times at ultrasonic wave 30KHz, temperature are 40 DEG C, each 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 10.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 60%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 35%, the weight content of sweet tea tannins is 16%.
Embodiment 9
Formula containing the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 96.5%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 4.
Embodiment 10
Formula containing the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 96.5%; Wherein, glycosides account for sweet tea extract weight 20%, sweet tea tannins accounts for 20% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 30%(v/v) ethanol, lixiviate 20min under temperature is 40 DEG C of conditions, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 10, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 20%, the weight content of sweet tea tannins is 20%.
Embodiment 11
Formula containing the chewing gum of sweet tea extract: sweet tea extract 1.5%, matrix 98.5%; Wherein, glycosides account for sweet tea extract weight 10%, sweet tea tannins accounts for 18% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 4.
Embodiment 12
Formula containing the chewing gum of sweet tea extract: sweet tea extract 1.5%, matrix 98.5%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 13
Formula containing the chewing gum of sweet tea extract: sweet tea extract 0.3%, matrix 99.7%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 14
Formula containing the chewing gum of sweet tea extract: sweet tea extract 0.5%, matrix 99.5%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 21% of sweet tea extract weight.
The preparation method of described sweet tea extract is with embodiment 3.
Embodiment 15
Formula containing the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, tartaric acid 1%; Wherein, glycosides account for sweet tea extract weight 30%, sweet tea tannins accounts for 19% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 10%(v/v) ethanol, lixiviate 2 times under ultrasonic wave 20KHz, temperature are 30 DEG C of conditions, each 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 10.8, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 30%, the weight content of sweet tea tannins is 19%.
Embodiment 16
Formula containing the chewing gum of sweet tea extract: sweet tea extract 3.5%, matrix 95.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 8.9%, sweet tea tannins accounts for 20.2% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, normal temperature lixiviate 2 times, each 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 9.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 60%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 8.9%, the weight content of sweet tea tannins is 20.2%.
Embodiment 17
Formula containing the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 5%, sweet tea tannins accounts for 5% of sweet tea extract weight.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 DEG C of water lixiviate 30min, merge extract, filter, concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 5%.
Embodiment 18
Formula containing the chewing gum of sweet tea extract: sweet tea extract 8%, matrix 91%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 7% of sweet tea extract weight.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasonic wave 40KHz, temperature are 70 DEG C, each lixiviate 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 12.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 7%.
Embodiment 19
Formula containing the chewing gum of sweet tea extract: sweet tea extract 2.5%, matrix 96.5%, malic acid 0.8%, peppermint oil 0.2%; Wherein, glycosides account for sweet tea extract weight 40%, sweet tea tannins accounts for 10% of sweet tea extract weight.
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasonic wave 40KHz, temperature are 60 DEG C, each lixiviate 20min, merge extract, filter, get filtrate recycling design, obtain sweet tea crude extract.
2) in sweet tea crude extract, adding limewash makes its pH value be 11, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous absorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 10%.
The preparation method of embodiment 1 ~ 13 and the chewing gum containing sweet tea extract described in reference examples 1,2 is that at 50 ~ 60 DEG C, be heated to its melting, stir evenly, cooling is candy batch by sweet tea extract and matrix mixing; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing, is sweet tea chewing gum.
Sweet tea extract, matrix and tartaric acid mix by the preparation method of the chewing gum containing sweet tea extract described in embodiment 15, and at 50 ~ 60 DEG C, be heated to its melting, stir evenly, cooling is candy batch; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing, is sweet tea chewing gum.
Sweet tea extract, matrix, malic acid and peppermint oil mix by the preparation method of the chewing gum containing sweet tea extract described in embodiment 16 ~ 19, and at 50 ~ 60 DEG C, be heated to its melting, stir evenly, cooling is candy batch; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing, is sweet tea chewing gum.
Experimental example
For showing that the chewing gum containing sweet tea extract of the present invention is to the preventive effect of carious tooth, applicant has carried out experiment in vitro research for the mechanism of action of this chewing gum to cariogenic bacteria (streptococcus mutans), is specifically reported as follows:
1, experimental technique
The preparation of 1.1 chewing gum solution and bacteria suspension
Experimental group: the chewing gum 25g getting the embodiment of the present invention 3 ~ 5 and reference examples 1 ~ 2 respectively, pulverized, be placed in test tube, then interpolation 100ml is made into chewing gum solution containing the TTY fluid nutrient medium (tryptone-tryptose-yeast extract medium) of the sucrose of 1% respectively.
Negative control group: adopt not containing the TTY fluid nutrient medium of this chewing gum.
By streptococcus mutans (S.mutans) after TSB culture medium (Trypsin Induced soybean broth) activates 3h, to be inoculated at MSB solid medium (light saliva Selective agar medium) 37 DEG C Anaerobic culturel 48 hours, picking colonies typical makes suspension in 5ml SPSS, harmonic length is 540nm place, OD value (Optical Density, OD) is the bacteria suspension of 1.0.
1.2, chewing gum solution impact that cariogenic bacteria is grown
Get 0.3ml bacterial suspension inoculation respectively in the chewing gum solution of 3ml variable concentrations, Anaerobic culturel 48 hours at 37 DEG C, and each test tube bacterium of difference collected by centrifugation, be placed in 2ml physiological saline, mixing, ultra-violet and visible spectrophotometer surveys the OD value that wavelength is 540nm place.
1.3, chewing gum solution is on the impact of S.mutans acid production
Get 3ml bacterial suspension inoculation respectively in the chewing gum solution of each concentration of equivalent, Anaerobic culturel 48 hours at 37 DEG C, and calculate the △ pH value before and after cultivating, and contrast with the △ pH value of negative control group.
1.4, chewing gum solution is on the impact of S.mutans adhesive attraction
Be the ratio of 10:1 in chewing gum solution and bacteria suspension, respectively by bacterial suspension inoculation in the chewing gum solution of each concentration, after all test tubes 30 ° of horizontal angle Anaerobic culturel 48h, by the bacterium adhering to test tube wall NaOH solution wash-out, measure the OD value of wavelength at 540nm place, and calculate adhesion inhibition rate, contrast with the adhesion inhibition rate of negative control group.
1.5, the extraction of glucosyltransferase (Glucosyltransferase, GTF) and the detection of water-insoluble exocellular polysaccharide (WIG)
Get each 3ml of chewing gum solution of variable concentrations, add S.mutans bacteria suspension 0.3ml, after Anaerobic culturel, inoculum is centrifugal, collect supernatant, add solid ammonium sulfate, make saturation degree reach 60%, after centrifugal, precipitation be dissolved in phosphate buffer and dialyse, centrifugal, supernatant is the thick enzyme of GTF.Get the thick enzyme of 0.1mlGTF, add 4ml distilled water, adopt Coomassie Brilliant Blue (Bradford method) to measure total protein content.Separately get 100 μ l0.5mmol/L sucrose solutions, the thick enzyme 0.1ml of GTF, add 0.1mmol/L sodium phosphate buffer, the cumulative volume of mixed liquor is made to reach 500 μ l, hatch 60min for 37 DEG C, be heated to cessation reaction after 100 DEG C of lasting 10min, add the mixing of 2.5ml distilled water, centrifugal, collect supernatant and adopt Smogyi method to measure reduced sugar amount in enzyme one substrate reactions liquid.Under GTF unit of activity (IU) is defined as standard conditions (37 DEG C reaction 1h per minute discharge enzyme amount needed for 1 μm of ol reduced sugar from sucrose), and calculate the Rate activity of enzyme.
Get inoculum centrifugal, abandon supernatant, taking precipitate adds the NaOH5ml of 0.5mol/L, centrifugal, get supernatant, add the absolute ethyl alcohol of its 3 times of volumes, centrifugal, precipitation is the water-insoluble exocellular polysaccharide that reaction generates, and measures the content of water-insoluble exocellular polysaccharide with anthrone method.Contrast with the enzymatic activity of S.mutans in negative control group and insoluble exocellular polysaccharide.
2, experimental result:
2.1, in above-mentioned experiment, in chewing gum solution, all have streptococcus mutans to grow, and along with the rising of glycosides content in sweet tea extract and the reduction of sweet tea tannins content, in solution, the concentration of streptococcus mutans increase.The OD value population mean of experimental group S.mutans is lower than negative control group (P<0.01), and its inhibition increases with the reduction of glycosides content in sweet tea extract and the increase of sweet tea tannins.Concrete outcome is in table 1.
Table 1 chewing gum solution is to the inhibiting OD value (X ± s) of streptococcus mutans
2.2, all have difference between △ pH (pH variable quantity) population mean before and after chewing gum solution experiments, in sweet tea extract, glycosides content is 5%, sweet tea tannins content is when being 21%, and the △ pH value variable quantity of S.mutans is minimum; Significantly lower than negative control group △ pH (P<0.001) between the population mean of experimental group △ pH value.Specifically in table 2.
Table 2 chewing gum solution produces the impact (X ± s) of acid to streptococcus mutans
2.3, along with the rising of glycosides content in sweet tea extract, the reduction of sweet tea tannins content, adhesion inhibition rate also decreases.Experimental group adheres to the population mean of the OD value of bacterium liquid significantly lower than negative control group group (p<0.01), and concrete outcome is in table 3.
Table 3 chewing gum solution is on the impact (X ± s) of Adherence of Streptococcus mutans
2.4, experimental group is on the impact of total protein, GTF enzyme activity, water-insoluble exocellular polysaccharide in S.mutans enzyme liquid
2.4.1 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, in S.mutans enzyme liquid, the content of total protein reduces on the contrary.In the chewing gum solution of experimental group variable concentrations, total protein content is on the contrary higher than negative control group group.Specifically in table 4.
Table 4 chewing gum solution is on the impact (X ± s) of total protein content in S.mutans enzyme liquid
2.4.2 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, the GTF activity of S.mutans raises gradually.The reduced sugar amount of negative control group, enzyme activity, Rate activity are all apparently higher than experimental group (p<0.001).Specifically in table 5.
Table 5 chewing gum solution is on the impact (X ± s) of GTF in S.mutans
2.4.3 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, the water-insoluble exocellular polysaccharide content of S.mutans synthesis is rising trend; The ability of the insoluble exocellular polysaccharide of negative control group S.mutans synthetic water is significantly higher than experimental group (p<0.001).Specifically in table 6.
Table 6 chewing gum solution is on the impact (X ± s) of the insoluble exocellular polysaccharide of S.mutans synthetic water
Through above-mentioned experiment, in the GTF enzymatic activity of experimental group and sweet tea extract, glycosides content is proportionate, be negative correlativing relation with sweet tea tannins content, when content one timing of sweet tea extract in chewing gum, to the suppression of S.mutans along with glycosides content raise, sweet tea tannins content reduces and reduces.
Claims (1)
1., containing the chewing gum of sweet tea extract, comprise matrix and sweet ingredient, it is characterized in that: described sweet ingredient is sweet tea extract, and it accounts for 2.5% of this gum; In described sweet tea extract, the weight content of glycosides is 5%, and the weight content of sweet tea tannins is 21%; Its preparation method is that at 50 ~ 60 DEG C, be heated to its melting, stir evenly, cooling is candy batch by sweet tea extract and matrix mixing; Candy batch is rolled into sugar-tablet, and cooling, stripping and slicing, to obtain final product.
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| CN104432410B (en) * | 2014-12-23 | 2017-07-21 | 贵州省健康茶科技有限公司 | A kind of Sweet tea preservative |
| CN106266021A (en) * | 2016-08-20 | 2017-01-04 | 陈爱梅 | A kind of preparation method and applications of sweet tea extract |
| CN109717445B (en) * | 2019-01-30 | 2022-10-18 | 湖南绿蔓生物科技股份有限公司 | Sweet taste composition and preparation method and application thereof |
| CN114223774A (en) * | 2021-12-24 | 2022-03-25 | 中国农业科学院都市农业研究所 | Sugar-free chewing gum containing Lithocarpus litseifolius extract and preparation method thereof |
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| JP2003088345A (en) * | 2001-09-14 | 2003-03-25 | Torigoe Flour Milling Co Ltd | Life prolonging agent for food |
| CN101669642A (en) * | 2009-09-23 | 2010-03-17 | 上海应用技术学院 | Fruity sweet tea effervescent tablets and preparation method thereof |
| CN102118982A (en) * | 2008-08-11 | 2011-07-06 | 罗蒂株式会社 | Deodorant composition comprising peroxidase and effective under neutral conditions |
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| JP2003088345A (en) * | 2001-09-14 | 2003-03-25 | Torigoe Flour Milling Co Ltd | Life prolonging agent for food |
| CN102118982A (en) * | 2008-08-11 | 2011-07-06 | 罗蒂株式会社 | Deodorant composition comprising peroxidase and effective under neutral conditions |
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