CN103191036B - Sweet tea extract-contained toothpaste - Google Patents
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Abstract
The invention provides sweet tea extract-contained toothpaste. The toothpaste comprises a sweetening agent, wherein the sweetening agent is a sweet tea extract which is 0.01-0.1% of the toothpaste based on weight, the sweet tea extract contains 5-40% of rubusoside, and 2-21% of weet tea polyphenol. The sweet ingredient in the sweet tea extract-contained toothpaste is the sweet tea extract, when the rubusoside content in the sweet tea extract is within the range of 5-40% and the sweet tea polyphenol in the sweet tea extract is within the range of 2-21%, the rubusoside and the sweet tea polyphenol interact to achieve the effect of well preventing tooth decay and improving oral cavity health, and when the sweet tea extract content in the toothpaste is certain, the effect of preventing the tooth decay of the toothpaste is improved along with the reduction of the rubusoside content and the increase of the sweet tea polyphenol content.
Description
Technical field
Of the present inventionly belong to daily-use chemical industry technical field, be specifically related to a kind of toothpaste containing sweet tea extract.
Background technology
Toothpaste is made up of abrasivus, wetting agent, surfactant, binding agent, spice, sweeting agent and water.Sweeting agent can improve the mouthfeel of toothpaste, general selection glucide or xylitol, and the sugariness of glucide is high, is 300 ~ 500 times of sucrose, but takes in for a long time or take in human health in a large number.Xylitol has the advantages such as sugariness is high, heat is low, but Excess free enthalpy xylitol, triglyceride in blood of human body can be caused to raise, cause coronary atherosclerosis, also likely cause the diseases such as abdominal discomfort, flatulence, diarrhoea.
Folium hydrangeae strigosae (Rubus Suavissmus S Lee) is the distinctive natural sweet taste plant in Guangxi, is also Gao Tian best in the world, low grade fever, safety non-toxic and have the sweet-tasting plant of health care.In Folium hydrangeae strigosae about containing more than 3.2% flavone compound, the sweet tea tannins of about 9% and the glycosides (Rubusoside) of 5 ~ 8%, in addition, also be rich in several amino acids and the necessary trace element of human body, there is higher nutritive value and health value.Glycosides is the sweet taste factor that Folium hydrangeae strigosae presents sweet taste mouthfeel, and it is the low in calories high sweet substance of the diterpene glycoside that steviol (Steviol) and glucose are combined into, and its molecular formula is C
32h
50o
12.The glycosides extracted from Folium hydrangeae strigosae is mouthfeel the best in sweet-tasting plant, and the pure degree of sweet taste is close to white sugar, and its sugariness is equivalent to 300 times of sucrose, is sugared Dai Pin best in the Nature.Glycosides can also suppress the activity of the glucosyltransferase (GTF) producing cariogenic Streptococcus mutans (S.mutans) growth and synthesis glucosan in oral cavity, thus suppress the water-insoluble glucan (WIG) of strong adhesion and the generation of acid, prevent the generation of dental plaque, reach the effect of caries prevention.Sweet tea tannins is active component important in Folium hydrangeae strigosae, comprise distinctive GOD type elladitannin, also comprise gallic acid, ellagic acid, 2-pyrone-4,6-dicarboxylic acids, brevifolin carboxylic acid, sanguisorbic acid dilactone, caffeic acid etc., having obvious antioxidation, mutation, reduction cholesterol, the effect that reduces low density lipoprotein, LDL in blood, suppress blood pressure to rise, suppress blood glucose to rise, suppress platelet aggregation, antibacterial, anti-food anaphylaxis, improve gastrointestinal microbiological environment, smelly eliminating, is find optimal natural antioxidant at present.Therefore, sweet tea extract is a kind of desirable sweeting agent, can be applied to toothpaste production field.
The document " glycosides and xylitol are to the comparative study of the insoluble extracellular polysaccharide of Streptococcus mutans synthetic water " being published in volume the 3rd phase in " Guangxi medical science " March the 32nd in 2010 take glycosides as raw material, by the tryptone-tryptose-yeast extract medium (TTY) containing 0.5% sucrose, doubling dilution is adopted to be made into the glycosides solution of 5 kinds of variable concentrations, be followed successively by 5g/100ml, 2.5g/100ml, 0.125g/100ml, 0.625g/100ml, 0.313g/100ml, the glycosides solution of above-mentioned concentration all creates certain inhibitory action to the insoluble extracellular polysaccharide of Streptococcus mutans synthetic water, in above-mentioned concentration range, along with the reduction of glycosides concentration, inhibition declines.The document " Folium hydrangeae strigosae tooth protection sheet is on the impact of glucosyltransferase and extracellular water-insoluble polysaccharide " being published in volume the 6th phase in " Guangxi medical science " June the 34th in 2012 integrates Sweet-tea tooth protection lozenge that the natural company limited of fine jade strength produces as raw material with Guilin, by the tryptone-tryptose-yeast extract medium (TTY) containing 1% sucrose, doubling dilution is adopted to be made into the solution of 5 kinds of variable concentrations, be followed successively by 5g/50ml, 2.5g/50ml, 1.250g/50ml, 0.625g/50ml, 0.313g/50ml, because in this Sweet-tea tooth protection lozenge, the content of glycosides is 5.5%, be converted into the concentration of glycosides, be followed successively by 0.275g/50ml, 0.138g/50ml, 0.068g/50ml, 0.034g/50ml, 0.017g/50ml, the Sweet-tea tooth protection lozenge of above-mentioned concentration has obvious inhibitory action to Streptococcus mutans GTF enzyme activity, also the synthesis of WIG can be suppressed, in above-mentioned concentration range, along with the reduction of glycosides concentration, inhibition declines.Comprehensively above-mentioned two sections of documents, we can draw a conclusion, and namely glycosides concentration is higher, stronger to the inhibition of Streptococcus mutans, otherwise, then more weak.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of toothpaste containing sweet tea extract, this toothpaste take sweet tea extract as sweet ingredient, when in sweet tea extract containing during compared with the glycosides of low dosage and higher dosage sweet tea tannins, good caries prevention can be reached and improve the effect of oral health.
Technical scheme provided by the invention is a kind of toothpaste containing sweet tea extract, and comprise sweeting agent, described sweeting agent is sweet tea extract, and it accounts for 0.2 ~ 2.5% of this toothpaste weight; In described sweet tea extract, the weight content of glycosides is 5 ~ 40%, and the weight content of sweet tea tannins is 2 ~ 21%.
Sweet tea extract of the present invention accounts for 2 ~ 2.5% of this toothpaste weight.
Sweet tea extract of the present invention accounts for 2.5% of this toothpaste weight.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 40%, and the weight content of sweet tea tannins is 7 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 40%, and the weight content of sweet tea tannins is 15 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 35%, and the weight content of sweet tea tannins is 16 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 30%, and the weight content of sweet tea tannins is 17 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 20%, and the weight content of sweet tea tannins is 18 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5 ~ 10%, and the weight content of sweet tea tannins is 18 ~ 21%.
In sweet tea extract of the present invention, the weight content of glycosides is 5%, and the weight content of sweet tea tannins is 21%.
Toothpaste of the present invention also comprises abrasivus, wetting agent, surfactant, binding agent, spice and water.Abrasivus can be calcium carbonate, dicalcium phosphate dehydrate, calcium phosphate dibasic anhydrous, calcium pyrophosphate, silicate hydrate or aluminium hydroxide.Wetting agent can be glycerol, propylene glycol or sorbitol.Surfactant generally adopts neutral detergent--sodium lauryl sulfonate, can foam, and the dirt in rinsing the mouth again, free from extraneous odour.Binding agent can to make in toothpaste batching be uniformly dispersed, and generally adopts sanlose and derivant thereof or sodium alginate.Spice is generally pennyroyal spice, specifically Oleum menthae, Mentholum, can give toothpaste and clearly feel, and can be also fruits essence, as citrus flavor etc.
Applicant finds, when employing glycosides content is 5 ~ 40%, during the sweeting agent of the sweet tea extract of sweet tea tannins content in the scope of 2 ~ 21% as toothpaste, toothpaste is made to have suitable sweet taste on the one hand, to adapt to the mouthfeel needs of part population, on the other hand, in sweet tea extract, glycosides and sweet tea tannins are with the contact process in oral cavity, synergism can be played, give full play to the effect of the activity of the glucosyltransferase (GTF) suppressing Streptococcus mutans growth and reduce synthesis glucosan, thus reach and suppress the water-insoluble glucan (WIG) of strong adhesion and telling on of acid, prevent dental plaque from producing, and then reach the effect of caries prevention.Applicant also finds, when content one timing of sweet tea extract in toothpaste, caries prevention effect is along with the rising of glycosides content in sweet tea extract, the reduction of sweet tea tannins content and reducing.
Detailed description of the invention
The present invention is further elaborated for following specific embodiment, but not as a limitation of the invention.
Following percent is percetage by weight.
Embodiment 1
Formula containing the toothpaste of sweet tea extract: sweet tea extract 0.2 part, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 27.8 parts, water.
In described sweet tea extract, the weight content of glycosides is 40%, sweet tea tannins is 21%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, at ultrasound wave 40KHz, temperature are 40 DEG C, reflux, extract, 2 times, extracts 20min, merge extractive liquid, at every turn, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 9, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 70%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 21%.
Embodiment 2
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2.5 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 25.5 parts, water.
In described sweet tea extract, the weight content of glycosides is 5%, the content of sweet tea tannins is 2%.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 DEG C of water lixiviates 2 times, each lixiviate 20min, merge extractive liquid, filter, concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 2%.
Embodiment 3
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26 parts, water.
In described sweet tea extract, the weight content of glycosides is 5%, sweet tea tannins is 21%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 10%(v/v) ethanol, room temperature lixiviate 2 times, each lixiviate 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) with D101 macroporous adsorbent resin, adsorbing separation is carried out to Folium hydrangeae strigosae crude extract, with 50%(v/v) ethanol elution, get eluent and concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 21%.
Embodiment 4
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26 parts, water.
In described sweet tea extract, the weight content of glycosides is 10%, sweet tea tannins is 18%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 30%(v/v) ethanol, room temperature lixiviate 2 times, each lixiviate 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) with D101 macroporous adsorbent resin, adsorbing separation is carried out to Folium hydrangeae strigosae crude extract, with 50%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 10%, the weight content of sweet tea tannins is 18%.
Embodiment 5
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26 parts, water.
In described sweet tea extract, the weight content of glycosides is 40%, sweet tea tannins is 15%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasound wave 40KHz, temperature are 40 DEG C, each lixiviate 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 11, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 70%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 15%.
Comparative examples 1
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26 parts, water.
In described sweet tea extract, the weight content of glycosides is 70%, sweet tea tannins is 7%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water mixing, adds the cellulase of sweet tea weight 0.3%, enzymolysis 2 hours under temperature 40 DEG C, pH4.5 condition, enzyme denaturing, filters, obtain filtrate;
2) filtrate is separated by the macromolecular filtering film that aperture is 10,000 molecular weight, obtains separating medium;
3) separating medium anion exchange resin is carried out adsorbing separation, obtain second time separating medium, again second time separating medium is carried out adsorbing separation by ADS-7 type macroporous adsorbent resin, then use 50%(v/v) ethanol elution, get eluent concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 70%, the weight content of sweet tea tannins is 7%.
Comparative examples 2
Formula containing the toothpaste of sweet tea extract: sweet tea extract 2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26 parts, water.
In described sweet tea extract, the weight content of glycosides is 95%, sweet tea tannins is 2%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, add water mixing, adds the cellulase of sweet tea weight 0.5%, the compound enzyme of hemicellulase, enzymolysis 3 hours under temperature 40 DEG C, pH4.5 condition, enzyme denaturing, filters, obtain filtrate;
2) be that the hollow ceramic film device that can retain 10,000 molecular weight carries out ultra-filtration and separation by filtrate by aperture, obtain trapped fluid and separating medium;
3) separating medium is carried out adsorbing separation by anion exchange resin, obtain second time separating medium, again second time separating medium is carried out adsorbing separation by ADS-7 type macroporous adsorbent resin, then use 70%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 95%, the weight content of sweet tea tannins is 2%.
Embodiment 6
Formula containing the toothpaste of sweet tea extract: sweet tea extract 1.5 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26.5 parts, water.
In described sweet tea extract, the weight content of glycosides is 35%, sweet tea tannins is 16%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, lixiviate 2 times at ultrasound wave 30KHz, temperature are 40 DEG C, each 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 10.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 60%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 35%, the weight content of sweet tea tannins is 16%.
Embodiment 7
Formula containing the toothpaste of sweet tea extract: sweet tea extract 0.5 part, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 27.5 parts, water.
In described sweet tea extract, the weight content of glycosides is 20%, sweet tea tannins is 20%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 20 orders, put into 30%(v/v) ethanol, lixiviate 20min under temperature is 40 DEG C of conditions, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 10, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 20%, the weight content of sweet tea tannins is 20%.
Embodiment 8
Formula containing the toothpaste of sweet tea extract: sweet tea extract 1.8 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26.2 parts, water.
In described sweet tea extract, the weight content of glycosides is 30%, sweet tea tannins is 19%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 10%(v/v) ethanol, lixiviate 2 times under ultrasound wave 20KHz, temperature are 30 DEG C of conditions, each 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 10.8, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 30%, the weight content of sweet tea tannins is 19%.
Embodiment 9
Formula containing the toothpaste of sweet tea extract: sweet tea extract 1 part, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 27 parts, water.
In described sweet tea extract, the weight content of glycosides is 8.9%, sweet tea tannins is 20.2%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 30 orders, put into 20%(v/v) ethanol, room temperature lixiviate 2 times, each 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 9.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 60%(v/v) ethanol elution, get eluent to concentrate, dry, be sweet tea extract, wherein, the weight content of glycosides is 8.9%, the weight content of sweet tea tannins is 20.2%.
Embodiment 10
Formula containing the toothpaste of sweet tea extract: sweet tea extract 0.3 part, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 27.7 parts, water.
In described sweet tea extract, the weight content of glycosides is 5%, sweet tea tannins is 5%.
The preparation method of described sweet tea extract:
Get sweet tea and be crushed to 5 orders, put into 80 DEG C of water lixiviate 30min, merge extractive liquid, filter, concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 5%, the weight content of sweet tea tannins is 5%.
Embodiment 11
Formula containing the toothpaste of sweet tea extract: sweet tea extract 1.2 parts, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 26.8 parts, water.
In described sweet tea extract, the weight content of glycosides is 40%, sweet tea tannins is 7%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasound wave 40KHz, temperature are 70 DEG C, each lixiviate 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 12.5, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 7%.
Embodiment 12
Formula containing the toothpaste of sweet tea extract: sweet tea extract 0.4 part, hydrogen phosphate dihydrate Ca45 part, glycerol 10 parts, sorbitol 10 parts, carboxymethyl cellulose 1 part, sodium laurylsulfate 5 parts, Mentholum 1 part, 27.6 parts, water.
In described sweet tea extract, the weight content of glycosides is 40%, sweet tea tannins is 10%.
The preparation method of described sweet tea extract:
1) get sweet tea and be crushed to 40 orders, put into 30%(v/v) ethanol, lixiviate 2 times at ultrasound wave 40KHz, temperature are 60 DEG C, each lixiviate 20min, merge extractive liquid, filter, get filtrate recycling design, obtain Folium hydrangeae strigosae crude extract.
2) in Folium hydrangeae strigosae crude extract, adding lime water makes its pH value be 11, and after contamination precipitation, elimination precipitates, and gets supernatant for subsequent use.
3), after regulating supernatant pH to neutrality, with D101 macroporous adsorbent resin, adsorbing separation is carried out to it, with 50%(v/v) ethanol elution, eluent is concentrated, dry, be sweet tea extract, wherein, the weight content of glycosides is 40%, the weight content of sweet tea tannins is 10%.
Embodiment 1 ~ 12 and the toothpaste described in comparative examples 1 ~ 2 prepare all according to a conventional method.
Experimental example
For showing that the toothpaste containing sweet tea extract of the present invention is to the preventive effect of dental caries, applicant has carried out experiment in vitro research for the mechanism of action of this toothpaste to cariogenic bacteria (Streptococcus mutans), is specifically reported as follows:
1, experimental technique
The preparation of 1.1 toothpaste solution and bacteria suspension
Experimental group: the toothpaste 125g getting the embodiment of the present invention 3 ~ 5 and comparative examples 1 ~ 2 respectively, pulverized, be placed in test tube, then interpolation 100ml is made into toothpaste solution containing the TTY fluid medium (tryptone-tryptose-yeast extract medium) of the sucrose of 1% respectively.
Negative control group: adopt not containing the TTY fluid medium of this toothpaste.
By Streptococcus mutans (S.mutans) after TSB culture medium (trypsinization soybean broth) activates 3h, to be inoculated at MSB solid medium (light saliva Selective agar medium) 37 DEG C Anaerobic culturel 48 hours, picking colonies typical makes suspension in 5ml physiological saline solution, harmonic length is 540nm place, optical density value (Optical Density, OD) is the bacteria suspension of 1.0.
1.2, toothpaste solution impact that cariogenic bacteria is grown
Get 0.3ml bacterial suspension inoculation respectively in the toothpaste solution of 3ml variable concentrations, Anaerobic culturel 48 hours at 37 DEG C, and each test tube antibacterial of difference collected by centrifugation, be placed in 2ml normal saline, mixing, ultra-violet and visible spectrophotometer surveys the OD value that wavelength is 540nm place.
1.3, toothpaste solution is on the impact of S.mutans acid production
Get 3ml bacterial suspension inoculation respectively in the toothpaste solution of each concentration of equivalent, Anaerobic culturel 48 hours at 37 DEG C, and calculate the △ pH value before and after cultivating, and contrast with the △ pH value of negative control group.
1.4, toothpaste solution is on the impact of S.mutans adhesive attraction
Be the ratio of 10:1 in toothpaste solution and bacteria suspension, respectively by bacterial suspension inoculation in the toothpaste solution of each concentration, after all test tubes 30 ° of horizontal angle Anaerobic culturel 48h, by the antibacterial adhering to test tube wall NaOH solution eluting, measure the OD value of wavelength at 540nm place, and calculate adhesion inhibition rate, contrast with the adhesion inhibition rate of negative control group.
1.5, the extraction of glucosyltransferase (Glucosyltransferase, GTF) and the detection of water-insoluble extracellular polysaccharide (WIG)
Get each 3ml of toothpaste solution of variable concentrations, add S.mutans bacteria suspension 0.3ml, after Anaerobic culturel, inoculum is centrifugal, collect supernatant, add solid ammonium sulfate, make saturation reach 60%, after centrifugal, precipitation be dissolved in phosphate buffer and dialyse, centrifugal, supernatant is the thick enzyme of GTF.Get the thick enzyme of 0.1mlGTF, add 4ml distilled water, adopt Coomassie Brilliant Blue (Bradford method) to measure total protein content.Separately get 100 μ l0.5mmol/L sucrose solutions, the thick enzyme 0.1ml of GTF, add 0.1mmol/L sodium phosphate buffer, the cumulative volume of mixed liquor is made to reach 500 μ l, hatch 60min for 37 DEG C, be heated to cessation reaction after 100 DEG C of lasting 10min, add the mixing of 2.5ml distilled water, centrifugal, collect supernatant and adopt Smogyi method to measure reducing sugar amount in enzyme one substrate reactions liquid.Under GTF unit of activity (IU) is defined as standard conditions (37 DEG C reaction 1h per minute discharge enzyme amount needed for 1 μm of ol reducing sugar from sucrose), and calculate the Rate activity of enzyme.
Get inoculum centrifugal, abandon supernatant, taking precipitate adds the NaOH5ml of 0.5mol/L, centrifugal, get supernatant, add the dehydrated alcohol of its 3 times of volumes, centrifugal, precipitation is the water-insoluble extracellular polysaccharide that reaction generates, and measures the content of water-insoluble extracellular polysaccharide with anthrone method.Contrast with the enzymatic activity of S.mutans in negative control group and insoluble extracellular polysaccharide.
2, experimental result:
2.1, in above-mentioned experiment, in toothpaste solution, all have Streptococcus mutans to grow, and along with the rising of glycosides content in sweet tea extract and the reduction of sweet tea tannins content, in solution, the concentration of Streptococcus mutans increase.The OD value population mean of experimental group S.mutans is lower than negative control group (P<0.01), namely when glycosides content in sweet tea extract 5 ~ 95% and sweet tea tannins in the scope of 2 ~ 21% time, have inhibition to the growth of Streptococcus mutans, and its inhibition increases with the reduction of glycosides content and the increase of sweet tea tannins content.Concrete outcome is in table 1.
Table 1 toothpaste solution is to the inhibiting OD value (X ± s) of Streptococcus mutans
2.2, all have difference between △ pH (pH variable quantity) population mean before and after toothpaste solution experiment, in sweet tea extract, glycosides content is 5%, sweet tea tannins content is when being 21%, and the △ pH value variable quantity of S.mutans is minimum; Significantly lower than negative control group △ pH (P<0.001) between the population mean of experimental group △ pH value.Specifically in table 2.
Table 2 toothpaste solution produces the impact (X ± s) of acid to Streptococcus mutans
2.3, along with the rising of glycosides content in sweet tea extract, the reduction of sweet tea tannins content, adhesion inhibition rate also decreases.Experimental group adheres to the population mean of the OD value of bacterium liquid significantly lower than negative control group group (p<0.01), and concrete outcome is in table 3.
Table 3 toothpaste solution is on the impact (X ± s) of Adherence of Streptococcus mutans
2.4, experimental group is on the impact of total protein, GTF enzyme activity, water-insoluble extracellular polysaccharide in S.mutans enzyme liquid
2.4.1 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, in S.mutans enzyme liquid, the content of total protein reduces on the contrary.In the toothpaste solution of experimental group variable concentrations, total protein content is on the contrary higher than negative control group group.Specifically in table 4.
Table 4 toothpaste solution is on the impact (X ± s) of total protein content in S.mutans enzyme liquid
2.4.2 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, the GTF activity of S.mutans raises gradually.The reducing sugar amount of negative control group, enzyme activity, Rate activity are all apparently higher than experimental group (p<0.001).Specifically in table 5.
Table 5 toothpaste solution is on the impact (X ± s) of GTF in S.mutans
2.4.3 along with the reduction of the rising of glycosides content, sweet tea tannins content in sweet tea extract, the water-insoluble extracellular polysaccharide content of S.mutans synthesis is rising trend; The ability of the insoluble extracellular polysaccharide of negative control group S.mutans synthetic water is significantly higher than experimental group (p<0.001).Specifically in table 6.
Table 6 toothpaste solution is on the impact (X ± s) of the insoluble extracellular polysaccharide of S.mutans synthetic water
Through above-mentioned experiment, in the GTF enzymatic activity of experimental group and sweet tea extract, glycosides content is proportionate with sweet tea tannins content is negative correlativing relation, when sweet tea extract content one timing in toothpaste, to the inhibition of S.mutans along with glycosides content increase, sweet tea tannins content reduces and declines.
Claims (2)
1., containing the toothpaste of sweet tea extract, comprise sweeting agent, it is characterized in that: described sweeting agent is sweet tea extract, and it accounts for 2% of this toothpaste weight; In described sweet tea extract, the weight content of glycosides is 5%, and the weight content of sweet tea tannins is 21%.
2., containing the toothpaste of sweet tea extract, comprise sweeting agent, it is characterized in that: described sweeting agent is sweet tea extract, and it accounts for 2% of this toothpaste weight; In described sweet tea extract, the weight content of glycosides is 10%, and the weight content of sweet tea tannins is 18%.
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| 茶多酚对变形链球菌的体外抗菌活性研究;梁文红等;《遵义医学院学报》;20040630;第27卷(第3期);第230-231页 * |
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