CN103141499B - Application of nerol in preparing banana flower thrip inducing agent - Google Patents

Application of nerol in preparing banana flower thrip inducing agent Download PDF

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Publication number
CN103141499B
CN103141499B CN201310102038.5A CN201310102038A CN103141499B CN 103141499 B CN103141499 B CN 103141499B CN 201310102038 A CN201310102038 A CN 201310102038A CN 103141499 B CN103141499 B CN 103141499B
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China
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nerol
banana
banana flowers
attractant
flowers thrips
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CN201310102038.5A
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Chinese (zh)
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CN103141499A (en
Inventor
刘奎
张帆
付步礼
邱海燕
伍祎
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中国热带农业科学院环境与植物保护研究所
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Priority to CN201310102038.5A priority Critical patent/CN103141499B/en
Publication of CN103141499A publication Critical patent/CN103141499A/en
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Abstract

The invention discloses an application of nerol in preparing a banana flower thrip inducing agent. Nerol is active as active component of the inducing agent. When the inducing agent is used for inducing the banana flower thrip, the volume percentage concentration of nerol is 10-20%. An experiment of inducing the banana flower thrip through a Y-shaped tube shows that when the inducing agent is a paraffin oil solution of nerol which is 10-20% in volume percentage concentration, an average inducing rate on banana flower thrip adult insects is 90.00-76.67%; and in a field induction experiment, within 10d of treating through the paraffin oil solution of nerol which is 10% in volume percentage concentration, the inducing effect on the banana flower thrip adult insects is remarkably higher than that on banana bud, yellow sticky colored card and water. Nerol can be used for indicating occurrence and occurrence quantity of banana flower thrip in advanced in bud stage, and indicating to use an insecticide to prevent and control the banana flower thrip in the field.

Description

The application of nerol in preparation banana flowers thrips attractant

Technical field

The present invention relates to a kind of novelty teabag of nerol, be specifically related to the application of nerol in preparation banana flowers thrips attractant.

Background technology

Banana flowers thrips [Thrips hawaiiensis (Morgan)] belongs to thrips Thripidae, flower thrips belongs to, it is the important pests at the plant florescences such as banana, citrus, banyan, extensively be distributed in east, the world, the Pacific region and southern US, occur in south China banana producing region serious in recent years.Banana flowers thrips is activity centre with petal in any of several broadleaf plants garden, is assembled, the thrips quantity of intrusion petal is increased sharply after flower bud taken out by banana by the external world.Because banana flowers thrips female adult pest hobby is spending interior children tender ovary epidermis position to lay eggs, worm's ovum surrounding plants tissue is because of irriate, and growth failure and the protuberance that expands, form coarse little blackspot at pericarp, has a strong impact on any of several broadleaf plants exterior quality really.

Banana flowers thrips is caused harm in banana flowers, very hidden, not easily finds, works the mischief when by the time finding.Produce upper in order to ensure banana exterior quality, the common practice be from banana take out flower bud just use insecticide control, sprayed a medicine every 3-5 days, until during banana cutting off male flower cluster bagging.Doing like this is compare insurance, but adds use cost and the labor cost of agricultural chemicals, too increases the pollution of environment.At present, a kind of method of effective monitoring banana flowers thrips population dynamic is lacked to instruct field medication.Production utilizes usually yellow haftplatte to lure banana flowers thrips, it is that yellow plate can cling other small insects that Problems existing mainly contains two: one, and polypide mixes, and is difficult to distinguish; Two is that yellow haftplatte can not play the attracting action stronger than banana flowers, is difficult to the technology as monitoring.

Nerol (cis-3,7-dimethyl-2,6-octadienols; Molecular formula: C 10h 18o, molecular weight: 154.25; CAS:106-25-2; Its chemical formula is such as formula I Suo Shi), colourless liquid is a kind of flavor of raw material with Rose Essentielle, is widely used in the allotment of diet, food, the high-grade essence of daily use chemicals.Nerol is also the middle product of other important spices of synthesis, and is the critical materials of these important spices of synthesis.At present, there is not yet the correlative study report that nerol is prevented and treated for banana flowers thrips.

Summary of the invention

The object of this invention is to provide a kind of novelty teabag of nerol.This novelty teabag is the application of nerol in preparation banana flowers thrips attractant.

The active component of described attractant is nerol.

Described attractant can be the paraffin oil of nerol.

The present invention protects nerol or described attractant preparing the application had in the product of luring banana flowers thrips function.In described product, nerol separately as active component, also can have with other active component preventing and treating banana flowers thrips and carry out compatibility jointly as active component.

In above-mentioned application, when using described product to lure banana flowers thrips, the concentration expressed in percentage by volume of nerol is 10%-20%, specifically can be 10%.

The present invention also provides a kind of method of preventing and treating banana flowers thrips, comprises the step using described attractant to lure banana flowers thrips.

The present invention also provides a kind of method of monitoring banana flowers thrips quantity, comprises the step using described attractant to lure banana flowers thrips.

In the above described two methods, when banana flowers thrips lured by the described attractant of described use, the concentration expressed in percentage by volume of nerol is 10%-20%, specifically can be 10%.

In above-mentioned application or method, described in lure banana flowers thrips for luring banana flowers thrips adult.

Experiment proves, lure in experiment what carry out banana flowers thrips with Y-piece, when attractant be concentration expressed in percentage by volume is the paraffin oil of the nerol of 10%-20%, 90.00%-76.67% is reached to the rate of on average luring of banana flowers thrips adult, and with using banana flowers as odor source and using concentration expressed in percentage by volume be 10% the paraffin oil of ethyl nicotinate also show as the comparing result of attractant, concentration expressed in percentage by volume is that the paraffin oil of the nerol of 10% on average lures rate higher to banana flowers thrips adult.Lure in land for growing field crops in experiment, by concentration expressed in percentage by volume be the nerol of 10% paraffin oil process 10 days in, to the attracting action of banana flowers thrips adult apparently higher than banana petal, Yellow sticky trap and water.The present invention can be applicable to banana and takes out the quantity that the flower bud phase indicates field banana flowers thrips whether to occur and occur in advance, and guides field use of insecticide to carry out the control of banana flowers thrips.

Accompanying drawing explanation

Fig. 1 is the schematic diagram that Y-piece connects wide-mouth bottle.Wherein, direction shown in arrow is the direction of gas flowing in pipe.

Embodiment

The experimental technique used in following embodiment if no special instructions, is conventional method.

Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.

Chemical reagent information used in following embodiment is as follows:

Nerol: like that (Shanghai) changes into industrial development Co., Ltd purchased from ladder is uncommon, catalogue is numbered N0077, purity > 98.0%.

Geraniol: like that (Shanghai) changes into industrial development Co., Ltd purchased from ladder is uncommon, catalogue is numbered G0027, purity > 96.0%.

β-citronellol: purchased from Tokyo HuaCheng Industry Co., Ltd, catalogue is numbered C0370, purity > 92.0%.

Adjacent anisaldehyde: like that (Shanghai) changes into industrial development Co., Ltd purchased from ladder is uncommon, catalogue is numbered A0479, and purity is 98%.

Benzaldehyde: purchased from Tokyo HuaCheng Industry Co., Ltd, catalogue is numbered B2379, purity > 98.0%.

3-phenylpropion aldehyde: purchased from Aladdin Chemistry Co.Ltd, catalogue is numbered 1105836, purity is 95%.

Artificial neroli oil: purchased from Tokyo HuaCheng Industry Co., Ltd, catalogue is numbered A0500, and purity is 99%.

Ethyl nicotinate: purchased from Aladdin Chemistry Co.Ltd, catalogue is numbered D1201024, purity is 99%.

Above-mentioned 8 kinds of compounds are the volatile liquid of tool.

Embodiment 1, nerol are to the attracting action of banana flowers thrips

One, laboratory test

1, the different compounds of variable concentrations are to the attracting action of banana flowers thrips

Be determined as follows 24 kinds of different attractants respectively to the attracting action of banana flowers thrips adult:

Nerol, geraniol, β-citronellol, adjacent anisaldehyde, benzaldehyde, 3-phenylpropion aldehyde, artificial neroli oil or ethyl nicotinate;

Concentration expressed in percentage by volume be 1% nerol, geraniol, β-citronellol, adjacent anisaldehyde, benzaldehyde, 3-phenylpropion aldehyde, artificial neroli oil or ethyl nicotinate paraffin oil;

Concentration expressed in percentage by volume be 10% nerol, geraniol, β-citronellol, adjacent anisaldehyde, benzaldehyde, 3-phenylpropion aldehyde, artificial neroli oil or ethyl nicotinate paraffin oil.

Concrete assay method is as follows: as shown in Figure 1, at Y-piece (internal diameter 0.5cm, the long 5cm of straight tube, two side arms pipe is isometric, for 5cm, angle 60 °) two side arms all connect the wide-mouth bottle (internal diameter 5cm at the bottom of bottle, internal diameter of the bottleneck are 2.5cm, bottle height 11cm) that odor source is housed, air filter tube and mass air flow sensor that active carbon is housed, control gas flow rate by mass air flow sensor, the gas that odor source is distributed is along the side arm tube at its place to Y-piece threeway place directed flow, wherein, odor source in the wide-mouth bottle be connected with Y-piece one side arm be a kind of attractant 10ul is dropped in 1 square centimeter filter paper on after filter paper is placed at the bottom of the bottle of wide-mouth bottle, odor source in the wide-mouth bottle be connected with another side arm be paraffin oil 10ul is dropped in 1 square centimeter filter paper on after filter paper is placed at the bottom of the bottle of wide-mouth bottle, the flow velocity of two side arms mass air flow sensor is all set to 60ml/min, it is one group with 5 banana flowers thrips [Thrips hawaiiensis (Morgan)] adult, put into the porch of described Y-piece straight tube, observe the set direction (three/a period of time that the distance that in 3min, adult moves ahead in the arm of the Y-piece left and right sides is more than or equal to this sidewall pipe range is designated as selection) of adult in 3min, statistics selects the adult quantity of the side arm tube (process pipe) being connected with attractant, the adult quantity of the side arm tube (control tube) of paraffin oil is connected with selection, often survey the direction that a composition worm exchanges a Y-piece two side arms, often kind of attractant measures 6 groups, and (different two groups in Y-piece two side arms direction are for once to repeat, repeat for totally 3 times), what calculate often kind of attractant on average lures rate (=selection enters adult quantity/adult sum × 100% of participating in the experiment in process pipe), result is as table 1, shown in table 2 and table 3.

Table 1, undiluted compound are to the attracting action of banana flowers thrips adult

Table 2, concentration expressed in percentage by volume are that each compound of 1% is to the attracting action of banana flowers thrips adult

Table 3, concentration expressed in percentage by volume are that each compound of 10% is to the attracting action of banana flowers thrips adult

Note: in table, worm amount is the sum repeated for 3 times, rate of on average luring is 3 mean ± standard errors repeated; If on average lure containing identical lowercase after rate between different disposal, then represent that two kinds of process are not remarkable in P ﹤ 0.05 difference; If without identical lowercase, then represent that two kinds of process are at P ﹤ 0.05 significant difference.

The result of table 1-3 shows: when attractant be concentration expressed in percentage by volume is the paraffin oil of the nerol of 10%, on average lures rate the highest to banana flowers thrips adult, reaches 90.00%.

2, the nerol of variable concentrations is to the attracting action of banana flowers thrips

Carry out according to the assay method in step 1, difference is: the paraffin oil of to be concentration expressed in percentage by volume the be nerol of 5%, 10%, 20% or 50% of the attractant in the wide-mouth bottle of Y-piece side, result is as shown in table 4.

The nerol of table 4, different volumes percentage concentration is to the attracting action of banana flowers thrips adult

Nerol concentration To participate in the experiment adult sum Adult quantity in corresponding side arm tube On average lure rate (%) 5% 30 12 40.00±0.00c 10% 30 27 90.00±0.00a 20% 30 23 76.67±3.33b 50% 30 11 36.67±6.67c

Note: in table, worm amount is the sum repeated for 3 times, rate of on average luring is 3 mean ± standard errors repeated; If on average lure containing identical lowercase after rate between different disposal, then represent that two kinds of process are not remarkable in P ﹤ 0.05 difference; If without identical lowercase, then represent that two kinds of process are at P ﹤ 0.05 significant difference.

3, with the contrast experiment of banana flowers as odor source

Carry out according to the assay method in step 1, difference is: the paraffin oil (being called for short 10% nerol) of to be concentration expressed in percentage by volume the be nerol of 10% of the attractant in the wide-mouth bottle of Y-piece side, loads the flower (referred to as banana flowers) of the in vitro opening of Brazilian any of several broadleaf plants in opposite side wide-mouth bottle; Add up attractant and banana flowers respectively and on average lure rate to banana flowers thrips adult, result is as shown in table 5.

Table 5, with banana flowers as the comparative test result of odor source

Note: in table, worm amount is the sum repeated for 3 times, rate of on average luring is 3 mean ± standard errors repeated; If on average lure containing identical lowercase after rate between different disposal, then represent that two kinds of process are not remarkable in P ﹤ 0.05 difference; If without identical lowercase, then represent that two kinds of process are at P ﹤ 0.05 significant difference.

The result of table 5 shows, concentration expressed in percentage by volume is that the paraffin oil of the nerol of 10% is significantly higher than banana flowers as the rate of on average luring of attractant to banana flowers thrips adult.

4, with the contrast experiment of 10% ethyl nicotinate as attractant

Carry out according to the assay method in step 1, difference is: the paraffin oil (being called for short 10% nerol) of to be concentration expressed in percentage by volume the be nerol of 10% of the attractant in the wide-mouth bottle of Y-piece side, the paraffin oil (10% ethyl nicotinate) of to be concentration expressed in percentage by volume the be ethyl nicotinate of 10% of the attractant in opposite side wide-mouth bottle; Add up different attractant respectively and on average lure rate to banana flowers thrips adult, result is as shown in table 6.

Table 6, with 10% ethyl nicotinate as the comparative test result of attractant

Note: in table, worm amount is the sum repeated for 3 times, rate of on average luring is 3 mean ± standard errors repeated; If on average lure containing identical lowercase after rate between different disposal, then represent that two kinds of process are not remarkable in P ﹤ 0.05 difference; If without identical lowercase, then represent that two kinds of process are at P ﹤ 0.05 significant difference.

The result of table 6 shows, concentration expressed in percentage by volume is that the paraffin oil of the nerol of 10% is significantly higher than as the rate of on average luring of attractant to banana flowers thrips adult the paraffin oil that concentration expressed in percentage by volume is the ethyl nicotinate of 10%.

Two, field trial

Select to plant the outdoor land for growing field crops that banana variety is " Brazilian any of several broadleaf plants ", test adopts random district group, Gong Shesange district group (repetition), and each district group establishes 4 communities (process), the area of each community is approximately 96 square metres, Banana tree plantation line-spacing is about 2m, spacing in the rows is about 2m.Respectively be treated to the 1-3 days after flower bud taken out by banana, hang different inducing substances respectively in the middle part of each male bud, specific as follows:

Process 1: hang the paraffin oil (10% nerol) that concentration expressed in percentage by volume is the nerol of 10%;

Process 2: hang water;

Process 3: hang Yellow sticky trap (50cm × 13cm, purchased from Hebi, Henan Jia Duo science, industry and trade Co., Ltd);

The the 1st, 4,7,10 day after hanging different inducing substances, record the banana flowers thrips quantity in each process, record the banana flowers thrips adult quantity of the single banana petal appearance that each process is hung, result gets the mean value of each repetition, as shown in table 7 simultaneously.

Table 7, field different disposal lure quantity to banana flowers thrips adult

Time 10% nerol Water Yellow sticky trap Single petal appearance 1d after process 4.67±0.33a 0c 1c 2.33±0.33b 4d after process 8±0.58a 0c 0c 3.00±0.00b 7d after process 8.67±0.33a 0c 0c 3.67±0.33b 10d after process 10.33±0.33a 0c 0c 5.67±0.33b

Note: if containing identical lowercase after same line number word, then represent that two kinds of process are not remarkable in P ﹤ 0.05 difference; If without identical lowercase, then represent that two kinds of process are at P ﹤ 0.05 significant difference.

The result of table 7 shows, in processing 10 days, concentration expressed in percentage by volume be the paraffin oil of the nerol of 10% in field to the attracting action of banana flowers thrips adult apparently higher than banana petal, Yellow sticky trap and water.

Claims (6)

1. a banana flowers thrips attractant, is characterized in that: described attractant is the paraffin oil of nerol, and the active component of described attractant is nerol, and the concentration expressed in percentage by volume of nerol is 10%-20%.
2. the application had in the product of luring banana flowers thrips function prepared by attractant described in claim 1.
3. application according to claim 2, is characterized in that: described in lure banana flowers thrips for luring banana flowers thrips adult.
4. prevent and treat a method for banana flowers thrips, it is characterized in that: comprise and use attractant described in claim 1 to lure the step of banana flowers thrips.
5. monitor a method for banana flowers thrips quantity, it is characterized in that: comprise and use attractant described in claim 1 to lure the step of banana flowers thrips.
6. the method according to claim 4 or 5, is characterized in that: described in lure banana flowers thrips for luring banana flowers thrips adult.
CN201310102038.5A 2013-03-27 2013-03-27 Application of nerol in preparing banana flower thrip inducing agent CN103141499B (en)

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CN106719909A (en) * 2016-11-12 2017-05-31 广东中迅农科股份有限公司 A kind of thrips attractant
CN107624807A (en) * 2017-09-10 2018-01-26 张月婵 A kind of fragrant thrips attractant
CN107927012A (en) * 2017-12-01 2018-04-20 中国热带农业科学院环境与植物保护研究所 Application of three kinds of plants essential oils in yellow chest thrips attractant is prepared
CN108323525A (en) * 2018-02-08 2018-07-27 青岛农业大学 Application of the nerol in preparing termiticides

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