CN103120121A - Method for determining thermo sensitive genetic male sterile line through sowing at different stages in cold water treatment period - Google Patents
Method for determining thermo sensitive genetic male sterile line through sowing at different stages in cold water treatment period Download PDFInfo
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- CN103120121A CN103120121A CN2013100572006A CN201310057200A CN103120121A CN 103120121 A CN103120121 A CN 103120121A CN 2013100572006 A CN2013100572006 A CN 2013100572006A CN 201310057200 A CN201310057200 A CN 201310057200A CN 103120121 A CN103120121 A CN 103120121A
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Abstract
The invention discloses a method for determining a thermo sensitive genetic male sterile line through sowing at different stages in a cold water treatment period. Seeds of a sterile line to be treated with cold water are divided into two parts, A and B, wherein the part A is sown on a certain day during April 20th and May 10th and served as an early sterile line, and the part B is sown 9-11 days after the part A is sown and used as a late sterile line; the parts A and B are sown and transplanted under normal field management, the plant morphological change of the part A is closely observed during the end of June and the beginning of July, and the part B is immediately and correspondingly barreled and put into a cold water treatment tank to be treated for 6-8 days and then is taken out during a flat pulvinus period of the part A; and the last and penultimate tassels which have a pulvinus distance of+/-1cm are selected, and the residual tassels are removed by scissors, so that the thermo sensitive genetic male sterile line is obtained. The method has the advantages of simplicity, efficiency, low cost, strong environmental controllability and convenience in operation.
Description
Technical field
The present invention relates to the new processing method field of rice heterosis utilization new material, is exactly that a kind of interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period.
Background technology
The double-hybrid rice strains kind has been gathered high yield, high-quality and the strong merit such as anti-, has obtained large-area popularization.Double-linear hybrid rice is based on temperature sensitive core sterile material and utilizes paddy rice hybrid vigour as maternal preparing hybrid Rice Combination, rice thermosensitive genie male sterile male sterile under long day hot conditions can be produced crossbreed, and under short day cryogenic conditions, male-fertile can be bred self.The expression of the sterility of temperature-sensitive sterile line depends on that to be in temperature sensitive sterile sensitive periods be pollen mother cell formation stage inducing to the long day high temperature of the external environment of pollen monokaryotic stage in Rice Panicle differentiation, yet season in full summer, the rice temp-sensing sterile line that is in sterile sensitive periods meets with the relative low temperature of accident, can not fully induce sterile and cause the great risk of hybrid seed production failure with regard to having, alleviating or avoiding this production risk is two to be the key that paddy rice is successfully promoted.
Seed selection is hanged down the fertility origin temp and is prevented the drift of origin temp, is that Rice Production is safely vital to ensureing two; Fertility-sensitive period is artificially created low temperature treatment, and to be in two of segregative generation be caryon sterile line, can seed selection be nuclear sterile rice to two of low origin temp, ensure the safety of Rice Production, same method process two be the former kind of groups of caryon sterile line will greatly alleviate the risk of origin temp drift.
Xu Mengliang etc. have proposed artificial water cooling pond, and to set low temperature treatment two be the method for temperature-sensitive sterile line, and the environment controllability is strong, and is easy to operate, is the seed selection of temperature-sensitive sterile line and prevents that temperature sensitive two-line sterile line drift all has tangible result two.Determine that two is that the concrete toeatment period of temperature-sensitive sterile line is the key of this technical operation, the present invention determines the rice thermosensitive genie male sterile cold water treatment simple and easy method in period.
Summary of the invention
A kind of interval sowing simple and easy, efficient, with low cost of purpose of the present invention is determined the temperature-sensitive sterile line cold water treatment method in period.
Above-mentioned purpose realizes by following scheme:
A kind of interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that:
(1) seed that will treat the male sterile line of cold water treatment is divided into two parts of A and B, A part is called the early stage male sterile line as observing the growing process mark post sowing in certain 1 day of April 20 to May 10, and B part is called the later stage male sterile line as the cold water treatment object in the sowing in after planting 9-11 days of A part;
(2) after A part and the vernalization of B part at field sowing, move into Plastic Drum after the 19-21 days length of time rice seedlings grow, subsequently by common technical management, treat that the plant growing process of A part material 80% left and right enters pulvinus section at ordinary times, B part material of correspondence is positioned over process in water cooling pond after 4-6 days immediately and takes out;
(3) chose down 1 leaf the same day and fell the pulvinus of 2 leaves and list apart from the tassel for ± 1cm, other tassels are rejected with scissors, and get final product.
Described a kind of interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that: the B part described in step (1) is called the later stage male sterile line as the cold water treatment object in the sowing in after planting 10 days of A part.
Described a kind of interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that: in step (3), B part is positioned over to process in water cooling pond took out afterwards in 5 days.
Principle of the present invention is: be namely the temperature sensitive period that the fertility of male sterile line transforms the period of rice thermosensitive genie male sterile cold water treatment, the pollen mother cell formation stage of namely Rice Panicle differentiation is to the pollen monokaryotic stage, corresponding to the 5th and 6 phases that fringe is grown, last 5-7 days; Showing as down 1 leaf when the 6th phase of fringe differentiation finishes on morphology aligns with the auricle of 2 leaves, be " pulvinus is flat ", when finishing take cold water treatment 5 days, " pulvinus is flat " as the best cold water treatment period of rice temp-sensing two-line sterile line, can reach that to screen two of low origin temp be caryon sterile line with prevent that two is the purpose of nuclear sterile rice genetic drift.Yet, be in rice plant impalpable on form of the 5th phase that fringe grows, make the cold water treatment of accurately determining temperature-sensitive sterile line very difficult period.
Table 1 rice young panicle differentiation and development process determination methods
The present invention divides the sowing of two phases with pending temperature sensitive dual purpose genic male sterile line, be separated by between two phases 10 days, the process of two phases fringe differentiation was separated by about 5 days, owing to differing 10 days on sowing time front and back phase, the environmental temperature of later stage male sterile line field growing will be higher than the male sterile line in early stage, the growing process of later stage male sterile line is relatively very fast, and identical dual-purpose male sterile line differs sowing in 10 days, and its growing process approximately differed about 5 days.Therefore, the sterile line material of sowing in early stage is in " pulvinus is flat " period that form is easy to observe, and the sterile line material of later stage sowing is in fringe phase beginning of the 5th phase of differentiation, and this is the caryon sterile line fertility-sensitive period just, carries out cold water treatment the best.
Beneficial effect of the present invention is: simple and easy, efficient, accurate, the environment controllability is strong, and is easy to operate.
Embodiment
A kind of interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period,
(1) seed that will treat the male sterile line of cold water treatment is divided into two parts of A and B, and A part is called the early stage male sterile line as observing the growing process mark post in sowing on April 30, and B part is called the later stage male sterile line as the cold water treatment object in the sowing in after planting 10 days of A part;
(2) after A part and the vernalization of B part at field sowing, move into Plastic Drum after 20 day length of time rice seedlings grow, subsequently by common technical management, treat that the plant growing process of A part material 80% enters pulvinus section at ordinary times, immediately B part material of correspondence is positioned over to process in water cooling pond and took out afterwards in 5 days;
(3) chose down 1 leaf the same day and fell the pulvinus of 2 leaves and list apart from the tassel for ± 1cm, other tassels are rejected with scissors, and get final product.
(4) pollen microscopic examination and bagging selfing after the heading of the B part male sterile line after the processing, the sterility of evaluation male sterile line namely gets temperature-sensitive sterile line.
Table 1 rice young panicle differentiation and development process determination methods
The present invention divides the sowing of two phases with pending temperature sensitive dual purpose genic male sterile line, be separated by between two phases 10 days, the process of two phases fringe differentiation was separated by about 5 days, owing to differing 10 days on sowing time front and back phase, the environmental temperature of later stage male sterile line field growing will be higher than the male sterile line in early stage, the growing process of later stage male sterile line is relatively very fast, and identical dual-purpose male sterile line differs sowing in 10 days, and its growing process approximately differed about 5 days.Therefore, the sterile line material of sowing in early stage is in " pulvinus is flat " period that form is easy to observe, and the sterile line material of later stage sowing is in fringe phase beginning of the 5th phase of differentiation, and this is the caryon sterile line fertility-sensitive period just, carries out cold water treatment the best.
Claims (3)
1. an interval sowing is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that:
(1) seed that will treat the male sterile line of cold water treatment is divided into two parts of A and B, A part is called the early stage male sterile line as observing the growing process mark post sowing in certain 1 day of April 20 to May 10, and B part is called the later stage male sterile line as the cold water treatment object in the sowing in after planting 9-11 days of A part;
(2) after A part and the vernalization of B part at field sowing, move into Plastic Drum after the 19-21 days length of time rice seedlings grow, subsequently by common technical management, treat that the plant growing process of A part material 80% left and right enters pulvinus section at ordinary times, B part material of correspondence is positioned over process in water cooling pond after 4-6 days immediately and takes out;
(3) chose the same day B part material fall 1 leaf with fall the pulvinus of 2 leaves and list apart from the tassel for ± 1cm, other tassels are rejected with scissors, and get final product.
2. a kind of interval sowing according to claim 1 is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that: the B part described in step (1) is in the sowing in after planting 10 days of A part
Be called the later stage male sterile line as the cold water treatment object.
3. a kind of interval sowing according to claim 1 is determined the temperature-sensitive sterile line cold water treatment method in period, it is characterized in that: in step (3), B part is positioned over to process in water cooling pond took out afterwards in 5 days.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009901A (en) * | 2015-07-23 | 2015-11-04 | 安徽省农业科学院水稻研究所 | Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method |
| CN108271683A (en) * | 2018-02-01 | 2018-07-13 | 安徽省农业科学院水稻研究所 | A method of utilizing the temperature sensitive permanent line with genic sterile of cold water temperature-controlling system breeding rice |
| CN112021107A (en) * | 2020-09-08 | 2020-12-04 | 安徽省农业科学院水稻研究所 | Production method of core seeds of two-line photo-thermo-sensitive male sterile line of rice |
| CN113079984A (en) * | 2021-04-08 | 2021-07-09 | 安徽省农业科学院水稻研究所 | Production method of rice two-line photo-thermo-sensitive male sterile line stock |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009901A (en) * | 2015-07-23 | 2015-11-04 | 安徽省农业科学院水稻研究所 | Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method |
| CN108271683A (en) * | 2018-02-01 | 2018-07-13 | 安徽省农业科学院水稻研究所 | A method of utilizing the temperature sensitive permanent line with genic sterile of cold water temperature-controlling system breeding rice |
| CN112021107A (en) * | 2020-09-08 | 2020-12-04 | 安徽省农业科学院水稻研究所 | Production method of core seeds of two-line photo-thermo-sensitive male sterile line of rice |
| CN113079984A (en) * | 2021-04-08 | 2021-07-09 | 安徽省农业科学院水稻研究所 | Production method of rice two-line photo-thermo-sensitive male sterile line stock |
| CN113079984B (en) * | 2021-04-08 | 2023-08-22 | 安徽省农业科学院水稻研究所 | Production method of rice two-line photo-thermo-sensitive male nuclear sterile line stock |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
| CN114830936B (en) * | 2022-04-24 | 2023-07-21 | 河南师范大学 | Method for thoroughly sterility of pollen of different fertility populations of BNS series thermosensitive genic male sterile wheat |
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