CN103119165B - 制备富集文库的方法 - Google Patents

制备富集文库的方法 Download PDF

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Publication number
CN103119165B
CN103119165B CN201180046388.3A CN201180046388A CN103119165B CN 103119165 B CN103119165 B CN 103119165B CN 201180046388 A CN201180046388 A CN 201180046388A CN 103119165 B CN103119165 B CN 103119165B
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structures
target
nucleic acid
binding
dna
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Chinese (zh)
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CN103119165A (zh
Inventor
N·J·V·汉森
A·B·克里斯滕森
L·K·拉森
F·A·斯勒克
L·K·彼得森
J·拉斯穆森-迪特沃斯特
P·布莱克斯克耶尔
T·H·汉森
J·霍尔姆奎斯特
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Vipergen ApS
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Vipergen ApS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201180046388.3A 2010-09-27 2011-09-01 制备富集文库的方法 Active CN103119165B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10180435 2010-09-27
EP10180435.9 2010-09-27
PCT/EP2011/065117 WO2012041633A1 (en) 2010-09-27 2011-09-01 A method for making an enriched library

Publications (2)

Publication Number Publication Date
CN103119165A CN103119165A (zh) 2013-05-22
CN103119165B true CN103119165B (zh) 2017-04-12

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CN201180046388.3A Active CN103119165B (zh) 2010-09-27 2011-09-01 制备富集文库的方法

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US (2) US20130288929A1 (https=)
EP (2) EP3399035B1 (https=)
JP (2) JP6193761B2 (https=)
CN (1) CN103119165B (https=)
CA (1) CA2808656C (https=)
DK (2) DK3399035T3 (https=)
IL (1) IL224694B (https=)
WO (1) WO2012041633A1 (https=)

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WO2012041633A1 (en) * 2010-09-27 2012-04-05 Vipergen A method for making an enriched library
WO2014088830A2 (en) * 2012-12-05 2014-06-12 The Regents Of The University Of California Screening of nucleic acid agents via particle display
CN104630202A (zh) * 2013-11-13 2015-05-20 北京大学 一种能够减小微量核酸物质整体扩增时产生偏倚的扩增方法
GB201420852D0 (en) * 2014-11-24 2015-01-07 Genevillage Kft Method
WO2017031378A1 (en) * 2015-08-18 2017-02-23 Krusemark Casey J Systems and methods for proteomic activity analysis using dna-encoded probes
AU2017259794B2 (en) 2016-05-02 2023-04-13 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
US11186836B2 (en) 2016-06-16 2021-11-30 Haystack Sciences Corporation Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation OLIGONUCLEOTID-DRIVEN COMBINATORY SYNTHESIS VERIFICATION MOLECULES AND ASSOCIATED MANUFACTURING AND USE PROCESSES
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
RU2020112854A (ru) 2017-11-30 2021-12-30 Арракис Терапьютикс, Инк. Фотозонды, связывающие нуклеиновые кислоты, и способы их применения
EP3914704B9 (en) * 2019-01-22 2023-10-04 Vipergen APS A method for screening of an in vitro display library within a cell
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
CN112941634B (zh) * 2019-12-10 2023-09-26 成都先导药物开发股份有限公司 通过dna编码化合物库筛选同时结合多个生物靶标的化合物的方法
US20240209350A1 (en) * 2021-04-26 2024-06-27 European Molecular Biology Laboratory High complexity microcompartment-based interaction screening
GB202317973D0 (en) 2023-11-24 2024-01-10 Nuclera Ltd Use of DNA encoded libraries

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP1123305A1 (en) 1998-10-19 2001-08-16 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
EP1423400B2 (en) 2001-03-19 2013-05-22 President and Fellows of Harvard College Evolving new molecular function
ATE371026T1 (de) 2001-06-20 2007-09-15 Nuevolution As Eine methode zur synthese von matrizenabhängigen molekülen
EP1670939B1 (en) * 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
CN1898257B (zh) * 2003-12-17 2010-06-23 普雷西斯药品公司 合成编码文库的方法
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006047791A2 (en) * 2004-10-27 2006-05-04 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library device and method for use
DK1809743T3 (da) * 2004-11-08 2009-05-04 Vipergen Aps Strukturel nukleinsyre-styret kemisk syntese
EP1828381B1 (en) 2004-11-22 2009-01-07 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries
WO2008103900A2 (en) 2007-02-23 2008-08-28 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
CA2704226A1 (en) * 2007-11-01 2009-05-07 The Arizona Board Of Regents On Behalf Of The University Of Arizona Cell-free methods for detecting protein-ligand interctions
US8153446B2 (en) 2008-05-23 2012-04-10 Kent State University Fluorogenic compounds converted to fluorophores by photochemical or chemical means and their use in biological systems
EP2452195A4 (en) * 2009-07-07 2012-12-05 Agency Science Tech & Res METHODS OF IDENTIFYING A PAIR OF LIAISON PARTNERS
WO2012041633A1 (en) * 2010-09-27 2012-04-05 Vipergen A method for making an enriched library

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DNA 展示技术的原理及应用;张永钢等;《国际生物制品学杂志》;20061231;126-129 *
STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro;Doi et al;《FEBS Letters》;19991231;227-230 *

Also Published As

Publication number Publication date
EP2622073B1 (en) 2018-04-18
JP2013540440A (ja) 2013-11-07
JP6193761B2 (ja) 2017-09-06
WO2012041633A1 (en) 2012-04-05
CA2808656A1 (en) 2012-04-05
DK3399035T3 (da) 2021-05-17
JP2017060513A (ja) 2017-03-30
IL224694B (en) 2018-08-30
US20130288929A1 (en) 2013-10-31
EP3399035A1 (en) 2018-11-07
IL224694A0 (en) 2013-07-31
CN103119165A (zh) 2013-05-22
US20170233726A1 (en) 2017-08-17
EP3399035B1 (en) 2021-02-17
DK2622073T3 (da) 2018-07-30
US10513700B2 (en) 2019-12-24
CA2808656C (en) 2019-12-03
DK2622073T5 (en) 2019-02-25
EP2622073A1 (en) 2013-08-07

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