CN103114077B - Process for extracting active SOD (Superoxide Dismutase) from wheat seedlings - Google Patents

Abstract

The invention discloses a process for extracting active SOD (Superoxide Dismutase) from wheat seedlings. The process comprises the following steps of: harvesting fresh wheat seedlings, sorting, cleaning, juicing, filtering, concentrating, spraying and drying, collecting powder, checking and accepting, and packaging and storing. According to the process disclosed by the invention, the time from harvesting the fresh wheat seedlings to collecting the powder is controlled within 5 hours. The whole process is a physical process, the mechanical degree is high, the extracting rate is high and the temperature is controlled to 25 DEG C to 45 DEG C. The preparation process disclosed by the invention can be used for efficiently extracting SOD in the wheat seedlings and sufficiently storing and keeping the function activities; besides, the process is green and environment-friendly, the wheat seedlings can be recycled and the residues can be used as feed, so that industrial pollution is avoided.

Description

A kind of technique extracting active SOD from wheat seeding

Technical field

The invention belongs to active substance of plant extractive technique field, is more particularly a kind of technique extracting active SOD from wheat seeding.

Background technology

Superoxide-dismutase (english abbreviation SOD) have the title of human body waste " street cleaner ", " anti-ageing king ", " beauty treatment favourite son ", being a kind of important active substances of promoting HUMAN HEALTH as free-radical scavengers, is also the important functional food ingredient of a class.As far back as the middle and later periods sixties, just have scientist from animal blood, to extract SOD successively, but the common defects that these SOD exist is that price is high, security is unstable.Therefore, World Health Organization WHO appeals from animal plasma, not extract SOD, and European Union has also announced progressively to stop using animal proteinum, advocates and uses vegetable-protein.

In recent years, the production technology of vegetable-protein achieves breakthrough, but the overwhelming majority is chemical solvents extraction process, and these class methods exist the sex change danger that SOD is chemically contaminated, generally can not obtain highly purified active plant SOD, and chemical solvents waste liquid can cause a hidden trouble to environment.In addition, some novel extraction methods, as: microwave radiation exaraction, ultrasonic extraction etc., also mostly rest on laboratory stage.For meeting the growing demand of market to vegetable active SOD, seeking material of vegetable origin widely, realizing the industrialization scale operation of highly effective and safe is the only way which must be passed, enterprise need give full play to own technical characterstic and advantage, study, reference, absorption modern technique and experience, the extractive technique of the plant SOD that constantly brings forth new ideas and technique.

CN95117689.7 discloses a kind of preparation technology of wheat seedling powder, and the product of production is mainly used in nutrient.SOD is to temperature sensitive, and more than 45 DEG C by inactivation, this technical process controls undesirable to production temperature and time, and production efficiency need to improve.Due to limited containing SOD number of plant, and in wheat seeding, the content of SOD, far away higher than other plant, therefore, must make full use of resource, and under reasonable terms, from wheat seeding, the work meaning of the active SOD of high efficiency extraction is far-reaching.

Summary of the invention

Technical problem to be solved by this invention is, overcomes deficiency of the prior art, provides a kind of raw material to be easy to get, the preparation technology extracting active SOD from wheat seeding of safe and efficient, environmental protection.

From wheat seeding, extract a technique of active SOD, this technical process comprises the following steps:

(1) raw material harvesting, sorting: gather in wheat seeding in wheat seeding the best harvest time (winter), Lao Ye, Huang Ye are removed in sorting, obtain fresh and tender part as target material, often criticize raw material harvesting, in sorting 0.5 hour used time of control;

(2) clean, broken, squeeze the juice: target material step (1) obtained drops into automatic turning cleaning equipment, surface impurity is removed through rinsing, spray, fresh wheat seeding after cleaning enters crusher to carry out physical wall breaking and squeezes the juice, and crusher rotating speed is 1440r/min; Material after fragmentation presses juice by juice extractor; Control in 1 hour used time;

(3) filter, concentrate: the pasty state wheat juice that step (2) is obtained filters through resonance filter, adopt single-effect vacuum cryoconcentration method by filtrate continuous concentration, controlling vacuum tightness is-0.090MPa to-0.095MPa, vaporization temperature is 40 DEG C to 45 DEG C, obtain the concentrated solution that active substance content is 15% to 20%, solution remains pH value 7.4 ~ 7.8; This step controls in 1.5 hours used times;

(4) spraying dry, receipts powder: adopt spray-drying process instantaneously that the concentrated solution that step (3) is obtained is carried out wink-dry granulation, the spray-drying tower height adopted is 10.3m, process 300L concentrated solution per hour, temperature in tower is kept to be 25 DEG C to 45 DEG C, moment charging is completely dried in 10 to 20 seconds, receive powder in time by cyclonic separator, receiving relative humidity between powder is 35% to 40%, and temperature remains on 15 DEG C to 25 DEG C; The operation of this step controls in 1.5 hours used times;

(5) check and accept, pack warehouse-in: by powder barrelling sealing obtained for step (4), warehouse-in preservation.

Further, in step (2), production plant envrionment temperature is natural room temperature 5-40 DEG C.

In step (4), by controlling inlet temperature and air outlet temperature, concentrated solution moisture evaporation in the spray drying tower can be made to reach balance with external heat.

In step (4), before spray-drying process, in dish juice concentrated solution, add appropriate maltodextrin, stir, powder sticky wall in spray-drying tower can be prevented.

The present invention compared with prior art, has the following advantages and effect: 1, the strict temperature control of whole process, under the equal control temperature of each link is suitable for the state of surviving in production process at SOD: 25 DEG C to 45 DEG C, substantially increases the extraction survival rate of SOD.

2, technique is simple, easy to operate, and whole process level of automation is high, time short, high efficiency, extraction time comparatively traditional method (1-2 days) greatly shorten more than 4/5, the used time is less than 5 hours, workable, remarkable in economical benefits.

3, the present invention only relates to physical process, comprises fragmentation, squeezes the juice, cryoconcentration, spraying dry, and whole process, without chemical reaction, effectively can preserve active components of plants, and SOD purity is high, and every 100 grams contain: superoxide-dismutase (SOD) >=2000u.

4, wheat seeding dregs can be used as feed and agricultural fertilizer, green, environmental protection, capable of circulation.

Active SOD extraction process of the present invention, has green, environmental protection, feature capable of circulation concurrently simultaneously, zero discharging of waste liquid in production process, and wheat seeding dregs can be used as poultry, cattle food and agricultural organic fertilizer.Therefore, the present invention has good economic and social benefit.

Embodiment

Now the present invention is described in further detail in conjunction with the embodiments

Embodiment 1

(1) in wheat seeding best harvest time, hand harvest's wheat seeding, removes Lao Ye, Huang Ye through sorting, obtains fresh and tender wheat seeding 2000kg, 30 minutes used times.

(2) fresh and tender wheat seeding is evenly dropped into automatic turning cleaning equipment, surface impurity is removed through rinsing, spray, fresh wheat seeding after cleaning enters cage mill through travelling belt to carry out physical wall breaking and squeezes the juice, and crusher rotating speed is 1440r/min, blade indention, two-sided blade; The mixed solution obtained after fragmentation presses juice by the 5T/H type juice extractor of band automatic deviation correction and automatic washing band function, obtains 50 minutes pasty state wheat juice used times.

(3) pasty state wheat juice filters to obtain clear liquid through resonance filter, adopt single-effect vacuum cryoconcentration method by clear liquid continuous concentration, obtain deep green concentrated solution, the vacuum tightness of vaporizer is-0.090MPa to-0.095MPa, by effective cooling water system, remain that the vaporization temperature of solution in vaporizer is 40 DEG C to 45 DEG C, gained concentrated solution pH7.8 is in alkalescence.1.5 hours this step used times.

(4) concentrated solution adopts spray-drying process instantaneously to carry out wink-dry granulation, spray-drying tower height is 10.3m, per hourly process 300L concentrated solution, keep temperature in tower to be 25 DEG C to 45 DEG C, moment charging is completely dried in 10 to 20 seconds, receives powder in time by cyclonic separator, produce end advance pedestrian work at this batch of material and sweep tower once, sweep 10 minutes tower used times, receiving relative humidity between powder is 38%, temperature 20 DEG C; Dry to receive when powder shares 1.5 hours.

(5) dry powder is weighed: 60kg, extraction yield is 3%, active SOD detected result: every 100 grams of dry powder are 2100u containing superoxide-dismutase (SOD), meet the requirements (every 100 grams contain: superoxide-dismutase (SOD) >=2000u), barrelling sealing warehouse-in is preserved.

Claims (2)

1. from wheat seeding, extract a technique of active SOD, it is characterized in that, this technique comprises the following steps:

(1) raw material harvesting, sorting: gather in wheat seeding in wheat seeding best harvest time, Lao Ye, Huang Ye are removed in sorting, obtain fresh and tender part as target material, often criticize raw material harvesting, in sorting 0.5 hour used time of control:

(2) clean, broken, squeeze the juice: target material step (1) obtained drops into automatic turning cleaning equipment, surface impurity is removed through rinsing, spray, fresh wheat seeding after cleaning enters crusher to carry out physical wall breaking and squeezes the juice, and crusher rotating speed is 1440r/min; Material after fragmentation presses juice by juice extractor, obtains pasty state wheat juice; This step controls in 1 hour used time;

(3) filter, concentrate: the pasty state wheat juice that step (2) is obtained filters through resonance filter, adopt single-effect vacuum cryoconcentration method by filtrate continuous concentration, controlling vacuum tightness is-0.090MPa to-0.095MPa, vaporization temperature is 40 DEG C to 45 DEG C, obtain the concentrated solution that solid matter content is 15% to 20%, solution remains pH value 7.4 ~ 7.8; This step controls in 1.5 hours used times;

(4) spraying dry, receipts powder: adopt spray-drying process instantaneously that the concentrated solution that step (3) is obtained is carried out wink-dry granulation, the spray-drying tower height adopted is 10.3m, process 300L concentrated solution per hour, temperature in tower is kept to be 25 DEG C to 45 DEG C, moment charging is completely dried in 10 to 20 seconds, receive powder in time by cyclonic separator, receiving relative humidity between powder is 35% to 40%, and temperature remains on 15 DEG C to 25 DEG C; The operation of this step controls in 1.5 hours used times;

(5) check and accept, pack warehouse-in: by powder barrelling sealing obtained for step (4), warehouse-in preservation.

2. technique according to claim 1, is characterized in that, in step (2), production environment temperature is natural room temperature 5-40 DEG C.