CN107988077A - A kind of fresh marigold flower cell membrane wall-breaking method - Google Patents
A kind of fresh marigold flower cell membrane wall-breaking method Download PDFInfo
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- CN107988077A CN107988077A CN201711143375.3A CN201711143375A CN107988077A CN 107988077 A CN107988077 A CN 107988077A CN 201711143375 A CN201711143375 A CN 201711143375A CN 107988077 A CN107988077 A CN 107988077A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
A kind of fresh marigold flower cell membrane wall-breaking method, including complex enzyme formulation is prepared, fresh flower broken wall, it is characterised in that by weight, 13 parts of fungal xylanases are taken, the ratio that 52 parts of endoglucanase is mixed into complex enzyme formulation；Foregoing complex enzyme formulation is used for fresh marigold flower broken wall, and between complex enzyme formulation is 0.005 0.03% relative to the dosage of fresh flower, in the case where temperature is 10 40 DEG C of ambient temperature conditions, stacking is fermented 35 days effects for reaching fresh flower broken wall.The enzyme preparation of addition can be quickly by fresh flower broken wall, and only needing 3 to 5 days can be complete by fresh flower broken wall, and the time, 1/6th equivalent to art methods broken time arrived a quarter, its lutein extraction rate reached at least adds 7% to 97.2%；Not only broken time had been shortened but also had reduced the yield of waste water, and shortened the production cycle of product and reduce cost.
Description
Technical field
The present invention relates to a kind of fresh marigold flower cell membrane wall-breaking method.
Background technology
Marigold plantation, processing, the process for the fresh marigold flower for extracting the purchase of Xanthophylls from Marigold manufacturing enterprise are：
Fresh flower is purchased --- and-blue or green store up is fermented（Breaking-wall cell）--- -- drying granulation ----lutein extracts.The process of wherein blue or green storage fermentation
More than 20 days are needed, principle is the biology enzyme that is produced in growth course using lactic acid bacteria by fresh flower cell wall lysis, is conducive to
Lutein is extracted more complete.The fresh flower lutein recovery rate handled without breaking wall by fermentation is 90% or so, existing for this method
Problem is：
1st, fermentation time is up to more than 20 days, it is impossible to carries out baking granulation processing to fresh flower in time；2nd, long-time storage has largely
Lactic Acid Wastewater discharge, the organic acid contained in waste water has environment certain adverse effect.
The content of the invention
The purpose of the present invention is disclosing a kind of fresh marigold flower cell membrane wall-breaking method, to solve at present from fresh marigold flower
The recovery rate of lutein is extracted only 90% or so, causes wastage of material, cost increases, and the process of green grass or young crops storage fermentation, time length is asked
Topic.Lutein recovery rate is up to 97% after broken wall treatment.
Technical solution：
A kind of fresh marigold flower cell membrane wall-breaking method, including complex enzyme formulation is prepared, fresh flower broken wall, i.e., is added in fresh flower multiple
Close fermented by biological enzyme；By weight, 1-3 parts of fungal xylanases are taken, the ratio that 5-2 parts of endoglucanase is mixed into complex enzyme
Preparation；Foregoing complex enzyme formulation is used for the mode of fresh marigold flower broken wall：Enzyme preparation is 0.005- relative to the dosage of fresh flower
Between 0.03%, in the case where temperature is 10-40 DEG C of ambient temperature conditions, stackingFermentationReach within 3-5 days the effect of fresh flower broken wall.
Preferably, by weight, fungi Mu Ju Tang Mei ︰ endoglucanases are taken to be mixed to prepare complex enzyme formulation for 1 ︰ 2；
Preferably, in fresh marigold flower broken wall process, by weight, with every 100 kilograms of fresh marigold flower complex enzyme systems
0.018-0.022 kilograms of agent.
The 2/3 of complex enzyme formulation used is used for fresh flower during the heap of Hua Chizhong, is watered uniform sprinkling;Another 1/3 uses
Flower raw material surface after heap is watered sprinkling.The foregoing amount of being watered is no less than 100 times of complex enzyme formulation weight.
Preferably, it is 25-35 DEG C to have used the environment temperature that the stacking of the fresh marigold flower of complex enzyme formulation is fermented.
The selection of stacking fermentation process carries out in unlimited fermentation flower pond.
Its broken wall situation to fresh marigold flower is detected, it turns out that, the enzyme preparation being directly added into can quickly will be fresh
Flower broken wall, only needing 3 to 5 days can be complete by fresh flower broken wall, and the time, 1/6th equivalent to usual method broken time arrived
Its lutein extraction rate reached of a quarter at least adds 7% to 97.2%；Not only shorten broken time but also reduce the production of waste water
Raw amount.Shorten the production cycle of product and reduce cost.
Embodiment
The application example of the present invention is given below：
First, complex enzyme formulation prepares example：
1st, 10 kilograms of fungal xylanases are taken, then take 50 kilograms of endoglucanases, are uniformly mixed to obtain 60 kilograms of complex enzyme formulation
It is spare.
2nd, 8 kilograms of fungal xylanases are taken, then take 16 kilograms of endoglucanases, are uniformly mixed to obtain the public affairs of complex enzyme formulation 24
Jin is spare.
3rd, 30 kilograms of fungal xylanases are taken, then take 70 kilograms of endoglucanases, are uniformly mixed to obtain complex enzyme formulation 100
It is kilogram spare.
4th, 20 kilograms of fungal xylanases are taken, then take 30 kilograms of endoglucanases, are uniformly mixed to obtain complex enzyme formulation 50
It is kilogram spare.
5th, 15 kilograms of fungal xylanases are taken, then take 40 kilograms of endoglucanases, are uniformly mixed to obtain complex enzyme formulation 55
It is kilogram spare.
6th, 20 kilograms of fungal xylanases are taken, then take 45 kilograms of endoglucanases, are uniformly mixed to obtain complex enzyme formulation 65
It is kilogram spare.
7th, 15 kilograms of fungal xylanases are taken, then take 45 kilograms of endoglucanases, are uniformly mixed to obtain complex enzyme formulation 60
It is kilogram spare.
2nd, fresh flower broken wall example：
1st, according to the dosage of flower pond size, in advance complex enzyme formulation used in estimation, such as 300 tons of colored ponds, it is contemplated that use complex enzyme system
60 kilograms of agent, wherein 2/3 collects for marigold flower raw material and is watered sprinkling into pond process, specifically enters pond in marigold flower raw material
When, complex enzyme formulation is sprayed when being thrown into, strives that the flower raw material into pond is all spread across.Another 1/3 complex enzyme formulation stays in Feng Chiqian and converts
Water is sprayed at fresh flower surface, and black film is covered after having sprayed.
The amount of being watered is no less than 100 times of complex enzyme formulation amount, under the premise of follow-up baking operation is to the upper limit of moisture requirement,
Water is converted much, sprinkling it is more uniform, effect is better.
Observation flower pond situation, natural environment temperature are not less than under conditions of 20 DEG C daily, and fresh marigold flower is 3 in fermentation vat
It can go out pond baking, and when natural environment temperature is less than 20 DEG C, longer fermentation times can go out pond barbecue by 4-5 days.
2nd, 200 tons of Hua Chi, using 38 kilograms of complex enzyme formulation, the marigold for broken wall is sprayed at by 1 method of example in two times
In fresh flower, 4.5 tons of water are taken, first 38 kilograms of complex enzyme formulations are blended into and are stirred into mixed liquor, then 4538 kilograms are mixed
Liquid is sprayed in the fresh marigold flower in pond at twice, and flower covering black film in surface in pond, 15 DEG C of environment temperature, ferments 5 days, open
The raw material that black film takes out broken wall enters baking operation.
3rd, 280 tons of Hua Chi, are mixed for the spray twice of fresh marigold flower with 70 kilograms of complex enzyme formulation with 7700 kg of water
Spill, then lid black film, in 30 DEG C of ferment under ambient temperature 3 days, take off film and take out and send dryer to toast.
According to the method described above, complex enzyme formulation is first produced, is used further to fresh marigold flower broken wall, during shortening broken wall greatly
Time and improve shell-broken effect, reach raising work efficiency, reduce the purpose of cost.
Claims (6)
1. a kind of fresh marigold flower cell membrane wall-breaking method, including complex enzyme formulation is prepared, fresh flower broken wall, it is characterised in that by weight
Gauge, takes 1-3 parts of fungal xylanases, the ratio that 5-2 parts of endoglucanase is mixed into complex enzyme formulation；Foregoing complex enzyme system
Agent is used for the mode of fresh marigold flower broken wall：Complex enzyme formulation relative to the dosage of fresh flower between 0.005-0.03%, in temperature
For under 10-40 DEG C of ambient temperature conditions, stackingFermentationReach within 3-5 days the effect of fresh flower broken wall.
2. a kind of fresh marigold flower cell membrane wall-breaking method according to claim 1, it is characterised in that by weight, take true
Bacterium Mu Ju Tang Mei ︰ endoglucanases are mixed to prepare complex enzyme formulation for 1 ︰ 2.
3. a kind of fresh marigold flower cell membrane wall-breaking method according to claim 1, it is characterised in that in fresh marigold flower
In broken wall process, by weight, with 0.018-0.022 kilograms of complex enzyme formulation of every 100 kilograms of fresh marigold flowers.
A kind of 4. fresh marigold flower cell membrane wall-breaking method according to claim 1, it is characterised in that complex enzyme system used
The 2/3 of agent is used for fresh flower during the heap of Hua Chizhong, is watered uniform sprinkling;Another 1/3 is used for the flower raw material surface after heaping,
It is watered sprinkling.
5. a kind of fresh marigold flower cell membrane wall-breaking method according to claim 4, it is characterised in that the foregoing amount of being watered is not
Less than 100 times of complex enzyme formulation weight.
6. a kind of fresh marigold flower cell membrane wall-breaking method according to claim 1, it is characterised in that used complex enzyme
The environment temperature of the stacking fermentation of the fresh marigold flower of preparation is 25-35 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711143375.3A CN107988077A (en) | 2017-11-17 | 2017-11-17 | A kind of fresh marigold flower cell membrane wall-breaking method |
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| CN201711143375.3A CN107988077A (en) | 2017-11-17 | 2017-11-17 | A kind of fresh marigold flower cell membrane wall-breaking method |
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| CN107988077A true CN107988077A (en) | 2018-05-04 |
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| CN201711143375.3A Pending CN107988077A (en) | 2017-11-17 | 2017-11-17 | A kind of fresh marigold flower cell membrane wall-breaking method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679934A (en) * | 2019-10-28 | 2020-01-14 | 青岛佳一生物科技股份有限公司 | Lutein-containing enzyme capable of clearing liver and improving eyesight and preparation method thereof |
| CN112175871A (en) * | 2020-10-12 | 2021-01-05 | 北京工商大学 | Marigold flower and fresh flower compound leavening agent and application thereof |
| CN112641825A (en) * | 2020-12-31 | 2021-04-13 | 赣江新区生鸿企业管理中心(有限合伙) | Traditional Chinese medicine composition mainly containing high-purity lutein for relieving eyestrain |
Citations (2)
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|---|---|---|---|---|
| CN1840686A (en) * | 2006-01-13 | 2006-10-04 | 江苏瑞迪生科技有限公司 | Process for preparing lutein fatty acid ester by enzyme degradation and solvent extraction method |
| CN106190555A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of cigarette Flos Tagetis Erectae spice |
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2017
- 2017-11-17 CN CN201711143375.3A patent/CN107988077A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840686A (en) * | 2006-01-13 | 2006-10-04 | 江苏瑞迪生科技有限公司 | Process for preparing lutein fatty acid ester by enzyme degradation and solvent extraction method |
| CN106190555A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of cigarette Flos Tagetis Erectae spice |
Non-Patent Citations (6)
| Title |
|---|
| H. B. SOWBHAGYA等: "Enzyme-Assisted Extraction of Flavorings and Colorants from Plant Materials", 《CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION》 * |
| HALAGUR B 等: "Effect of pretreatments on extraction of pigment from marigold flower", 《JOURNAL OF FOOD SCIENCE AND TECHNOLOGY》 * |
| JOSÉ LUIS NAVARRETE-BOLANTILDEOS 等: "Improving Xanthophyll Extraction from Marigold Flower Using Cellulolytic Enzymes", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| 庄苏 等: "纤维素酶与木聚糖酶对象草青贮发酵品质的影响", 《南京农业大学学报》 * |
| 林筱璐 等: "添加复合酶制剂对香蕉叶青贮品质的影响", 《家畜生态学报》 * |
| 蒋旺: "酶辅助若干中药有效成分的提取", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679934A (en) * | 2019-10-28 | 2020-01-14 | 青岛佳一生物科技股份有限公司 | Lutein-containing enzyme capable of clearing liver and improving eyesight and preparation method thereof |
| CN112175871A (en) * | 2020-10-12 | 2021-01-05 | 北京工商大学 | Marigold flower and fresh flower compound leavening agent and application thereof |
| CN112641825A (en) * | 2020-12-31 | 2021-04-13 | 赣江新区生鸿企业管理中心(有限合伙) | Traditional Chinese medicine composition mainly containing high-purity lutein for relieving eyestrain |
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Application publication date: 20180504 |