Background technology
Yuan root (Brassica rapa L.) has another name called turnip, turnip, for Cruciferae (Cruciferae) Btassica (Brassica) herbaceous plant, be have hide cultural features food, medicine, raise three use plants, grow in high altitude localities more than height above sea level 3500m, the ground such as the Qushui River, main product Tibet, a surname's language " Niu Ma " by name, the plantation history of existing more than 1000 year, is rich in the nutritional factors of multiple needed by human body and special functional component.The Yuan root that the Tibetanmedicine masterpiece Four-Volume Medical Code is recorded, the circular radish of outer likeness in form, individual little taste is sweet, packing is high, taste is sweet, warm in nature, has effect of clearing away summerheat, nourishing oxygenation, reducing blood lipid, can play significant mitigation to anoxic, fatigue, the symptom such as not acclimatized.
2007, Dalian Light Industry College applied for " utilizing the preparation of Yuan root to have the method for the product of anti-anoxic, anti-fatigue effect " [Zhu Beiwei etc., application number is 200710090934.9], existing authorized.This patent relates to the muddy juice that Yuan root fresh goods is obtained after cleaning, remove the peel, precook, pulling an oar, by allotment, homogeneous, the beverage series that filling, sterilization obtains anti-anoxic, antifatigue.And claim to add several rare Chinese herbal medicines such as fern fiber crops, safflower, rhodiola root and apple, pear and other fruits, to increase local flavor and effect of product.
Experimental study in recent years has further confirmed the oxygen lack resistant function of Yuan root, and announcement may be mainly the contribution of Yuan root polysaccharide.As superfine in the people [Xie Yue such as the Xie of Sichuan University Yue in 2009, horse, the Primary Study of Tibet yuenkanin extract to mouse oxygen lack resistant function. Sichuan animal, 2009,28 (6): 853-856] studied the oxygen lack resistant function of yuenkanin extract to mouse, shown that all the resistance to the survival time under hypoxic condition of normal pressure, Ischemia Injury in Brain anoxic time, poisoning time-to-live of natrium nitrosum and the mouse peripheral blood of energy significant prolongation mouse resemble middle red blood cell number and content of hemoglobin for Yuan root water extract and alcohol extract.The patent of people's applications such as the Jiang Siping of Tibetan Autonomous Region Plateau Biology Institute discloses the purposes that a kind of Yuan of utilization root polysaccharide is prepared anti-anoxic medicine, claim the mean molecule quantity of Yuan root polysaccharide between 3-8 ten thousand, there is significant anti-hypoxia activity, can be used for prevention or treatment oxygen deprivation anoxic, hemic hypoxia, circulatory hypoxia and Poisoning anoxic [Jiang Siping, Gao Ping, Yuan root polysaccharide is for the preparation of the purposes of the medicine of anti-anoxic, CN200910082294.6].
Bamboo extractive (being bamboo-leaves flavones) is a kind of new bio flavones preparation of China's independent research, except have good Green Tea Extract, anti-oxidant, strengthen immunity, effect of delaying senility, also there is significant resistance to anoxic, antifatigue and anti-stress effect.Shenyang Pharmaceutical University pay the system research that dawn, spring etc. carried out [oxygen lack resistant function of bamboo extractive, new Chinese medicine and clinical pharmacology 2005,16(2): 100-102; The protective effect of bamboo extractive to hypoxic-ischemic cardiac muscle, Chinese patent drug 2006,28(4): 558-560; The protective effect of bamboo leave extract on cardiomyocytes injuried by hypoxia/reoxygenation, modern food and medicine magazine 2006,16(1): 18-21; The protective effect of bamboo extractive to myocardial ischemia-reperfusion injury, research and development of natural products 2006,18:214-218; Bamboo extractive is prevented and treated the pharmacodynamic study of cerebral ischemia, Traditional Chinese Medicine University Of Guangzhou's journal 2006,23(6): 492-495; The research of bamboo leave flavone Chinese People's Anti-Japanese Military and Political College rat cardiomyocyte apoptotic effect, combination of Chinese tradiational and Western medicine cardiovascular and cerebrovascular diseases magazine 2007,5(2): 125-126] show that the mouse survival time under bamboo-leaves flavones energy significant prolongation normobaric hypoxia condition significantly improves the anti-anoxia ability of whole animal; The cardiac muscle of acute ischemia, anoxic is produced to obvious protective effect, activities of antioxidant enzymes in its mechanism of action and its antagonism radical damage, reinforcement, reduce MCO, to reduce heart stalk area relevant.
Along with being open to traffic of Qinghai-Tibet Railway, the visitor's that enters into Tibet quantity is constantly soaring in recent years, and acute high altitude reaction becomes a large obstacle of tourist industry.Meanwhile, along with the continuous propelling that Tibetan area economy is built, the crowd of the work of more and more entering into Tibet needs altitude acclimatization.In addition, also have the soldier on a large amount of army limit, live in high altitude localities throughout the year, in the urgent need to the functional food of anti-anoxic and antifatigue, auxiliary its adapts to the severe extreme climate in plateau as early as possible.So distinctive Yuan Root Resource is support take Tibetan area, exploitation high-quality, efficient functional food have very wide market prospects.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of food compositions with hypoxia tolerance, and this food compositions has good effect at aspects such as anti-acute anoxia and/or prevention altitude sicknesses.
In order to solve the problems of the technologies described above, the invention provides a kind of food compositions with hypoxia tolerance, this food compositions comprises the function ingredients of following weight portion: Yuan root powder 30-80 part and bamboo-leaves flavones 20-40 part.
Improvement as the food compositions with hypoxia tolerance of the present invention: this food compositions is made up of the function ingredients of following weight portion: Yuan root powder 30-80 part, bamboo-leaves flavones 20-40 part, vitamin C 5-20 part and vitamin(e) C palmitate 5-10 part.
Further improvement as the food compositions with hypoxia tolerance of the present invention: the preparation method of Yuan root powder for following any one:
Mode A, dry Yuan root stem tuber (moisture content≤10%) is pulverized, obtained Yuan root powder (also a name Yuan root shines dry powder);
Mode B, fresh Yuan root stem tuber cleaned successively, cuts into slices, dehydrated and pulverizes, obtaining Yuan root powder (the also full powder of name Yuan root, moisture content≤5%; In the time that the mode of dehydrating is selected vacuum freeze drying, called after: Yuan root freeze-dried powder);
Mode C, dry Yuan root stem tuber (moisture content≤10%) is extracted, the extract of gained, successively through concentrated, dry, obtains Yuan root powder (also name yuenkanin extract powder);
Aforesaid way B is optimal way.
Further improvement as the food compositions with hypoxia tolerance of the present invention: in mode B: dehydrate as vacuum drying, vacuum microwave drying, vacuum freeze drying or vacuum microwave freeze drying.
Further improvement as the food compositions with hypoxia tolerance of the present invention: in mode C: the dry water of Yuan root stem tuber or alcohol-water mixture are carried out to refluxing extraction according to the solid-liquid ratio of 1 g:5 ~ 20 mL; Be dried as vacuum drying, vacuum microwave drying, vacuum freeze drying or spraying are dried, obtain Yuan root powder (also name yuenkanin extract).
Remarks: the said extracted time is generally 2 ~ 6 hours.
Further improvement as the food compositions with hypoxia tolerance of the present invention: food compositions is made up of the function ingredients of following weight portion: Yuan root powder 50-60 part, bamboo-leaves flavones 25-30 part, vitamin C 10-15 part, vitamin(e) C palmitate 5-10 part.
Further improvement as the food compositions with hypoxia tolerance of the present invention: food compositions is made up of the function ingredients of following weight portion: 55 parts, Yuan root powder, 25 parts of bamboo-leaves flavones, 15 parts of vitamin Cs, 5 parts of vitamin(e) C palmitates.
Further improvement as the food compositions with hypoxia tolerance of the present invention: Yuan root powder adopts mode B preparation and obtains dehydrating as vacuum freeze drying in mode B.
The food compositions with hypoxia tolerance of the present invention, bamboo-leaves flavones, vitamin C and vitamin(e) C palmitate all can obtain by commercial mode; Yuan root powder can obtain according to any one preparation in 3 kinds of modes provided by the invention.
For example: bamboo-leaves flavones (having another name called bamboo extractive) is commercially available prod, can select the product of Hangzhou Youmeite Technology Co., Ltd., product code is BLF-d24 [outward appearance brown color powder, active ingredient index is: general flavone (in rutin) >=24%, Lutonaretin >=2%, p-Coumaric Acid >=0.5 %] or BLF-b12 [outward appearance is brown color powder, and active ingredient index is: general flavone (in rutin) >=12%].
For the preparation of the raw material of Yuan root powder---Yuan root stem tuber is done or fresh Yuan root stem tuber can be purchased in the Tibetan family of Tibetan area.
The food compositions with hypoxia tolerance of the present invention, only need be simply uniformly mixed Yuan root powder, bamboo-leaves flavones, vitamin C and vitamin(e) C palmitate.
The food compositions with hypoxia tolerance of the present invention, in every 100 g, general flavone content is not less than 2000 mg, and polyoses content is not less than 2000 mg.
The food compositions with hypoxia tolerance of the present invention is pulvis, direct-edible; Also can add on its basis suitable auxiliary material, thus granulation agent, capsule or tablet; Auxiliary material can comprise the conventional auxiliary materials such as microcrystalline cellulose, starch, dolomol, dextrin, sodium carboxymethylcellulose.
The food compositions adult suggestion dose with hypoxia tolerance of the present invention is generally every day 1-2 time, each 0.5-3.0 g.
The food compositions with hypoxia tolerance of the present invention of often ingesting, can effectively promote the tolerance of human body to low-oxygen environment, and prevention plateau does not accommodate the generation of acute high altitude sickness.
Composition of the present invention tool compared with existing anti-anoxic product on the market has the following advantages:
By the Yuan root with nourishing oxygenation effect with there is Green Tea Extract, bamboo-leaves flavones anti-oxidant, that improve myocardial anoxia, protection cardiovascular and cerebrovascular carries out composite, be aided with vitamin C and ester class thereof simultaneously, bring into play significant synergistic function, can effectively promote the tolerance of human body to low-oxygen environment, prevention plateau discomfort; Can not only alleviate the crowd's of entering into Tibet acute high altitude reaction, the protection that is also conducive to plateau generation and occupies crowd's chronic plateau sickness., bamboo culture of long standing and well established to the geographical sign product of Tibetan of China (Yuan root) and China is merged mutually, product has more market affinity meanwhile, is easy to accept into consumers in general.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated, but the present invention is not limited to embodiment.
Embodiment 1: the preparation of Yuan root powder
Embodiment 1-1: choose the Tibet Yuan root stem tuber fresh goods that profile is neat, nothing is gone rotten, do not felt like jelly, clean, be cut into the thin slice of 5 mm left and right, spread out in dish, put into vacuum freeze drier (vacuum: 100Pa; Temperature :-28 ℃ ~-30 ℃; Temperature of heating plate: 65 ℃-70 ℃; Time: 16 h), collect dry complete Yuan heel piece, be crushed to 80 order left and right, obtain Yuan root freeze-dried powder (moisture is less than 5%).
Embodiment 1-2: get the homemade Yuan root of Tibetan stem tuber dry (moisture is 10% left and right), be crushed to 80 order left and right, obtain Yuan root and shine dry powder (moisture is in 10% left and right).
Embodiment 1-3: get dry (moisture is 10% left and right) 1 kg of the homemade Yuan root of Tibetan stem tuber, be ground into 10-20 object meal, add the pure water of 10 L, 2 h are extracted in hot reflux, temporary after extracting liquid filtering, (said extracted process repeats once, substitute the multiple said extracted of Yuan root stem tuber dry weight with filter residue), merge filtrate twice, be evaporated to solid content 25 % left and right, put into vacuum drier dehydration (45Pa, 45 ℃, 25h), be dried rear taking-up material, be crushed to 80-100 order left and right, obtain yuenkanin extract 0.64 kg (moisture 5% left and right), for the dry water extract powder of Yuan root stem tuber.
Embodiment 2: preparation has the food compositions of hypoxia tolerance
Embodiment 2-1: take Yuan root and shine dry powder 7.0 kg, bamboo-leaves flavones (BLF-b12) 2.0 kg, vitamin C 0.5 kg, vitamin(e) C palmitate 0.5 kg, puts into respectively cone-type mixer, mixes rear packing, becomes oral pulvis.
In every kg pulvis, contain general flavone 25 g, polysaccharide 45 g.Adult's suggestion dose is every day 1-2 time, each 1.0-3.0 g.
Embodiment 2-2: take Yuan root freeze-dried powder 4.0 kg, bamboo-leaves flavones (BLF-d24) 3.0 kg, vitamin C 2.0 kg, vitamin(e) C palmitate 1.0 kg, medical starch 1.0 kg, after putting into cone-type mixer and mixing, add 95% ethanol to make softwood as adhesive 0.1 kg, granulate with 20 eye mesh screens, wet grain is dried at 50 ℃.Collect dry particle, add microcrystalline cellulose (1.0 %, microcrystalline cellulose is 1.0 % with the mass ratio of dry particle) to mix, with the whole grain of 14 eye mesh screens, obtain granule.In every kg granule, contain general flavone 64.5 g, polysaccharide 21.1 g.Adult suggestion dose is every day 1-2 time, 0.5-2.0 g at every turn.
Above-mentioned granule is packed in hard shell capsules, capsule; Add again the lubricants such as dolomol routinely method make tablet.
Embodiment 2-3
Taking 0.55 kg Yuan root freeze-dried powder, 0.25kg bamboo-leaves flavones (BLF-d24), 0.15 kg vitamin C and 0.05 kg vitamin(e) C palmitate evenly mixes.
Embodiment 2-4
Taking 0.55 kg Yuan root solarization dry powder, 0.25kg bamboo-leaves flavones (BLF-d24), 0.15 kg vitamin C and 0.05 kg vitamin(e) C palmitate evenly mixes.
Embodiment 2-5
Taking 0.55 kg yuenkanin extract, 0.25kg bamboo-leaves flavones (BLF-d24), 0.15 kg vitamin C and 0.05 kg vitamin(e) C palmitate evenly mixes.
Experiment 1: the functional verification of composition
For investigating the hypoxia tolerance of the present composition, by embodiment 2-3 preparation and the food compositions with hypoxia tolerance (being designated hereinafter simply as pulvis A), by embodiment 2-4 preparation and the food compositions with hypoxia tolerance (being designated hereinafter simply as pulvis B), by embodiment 2-5 preparation and the food compositions with hypoxia tolerance (being designated hereinafter simply as pulvis C) carry out anti-anoxia ability research.
(1) animal used as test: healthy male ICR mouse, body weight (22 ± 2) g, is provided by Zhejiang University's animal center; Before experiment, animal adapts to 5 days.
(2) experiment grouping: Normal group, pulvis A group, pulvis B group, pulvis C group, 15 every group; Totally 7 days;
Normal group difference every day gavage distilled water 0.5 mL/ only;
Pulvis A organizes distilled water 0.5 mL/ that every day, gavage contained 3.0 mg pulvis A respectively;
Pulvis B organizes distilled water 0.5 mL/ that every day, gavage contained 3.0 mg pulvis B respectively;
Pulvis C organizes distilled water 0.5 mL/ that every day, gavage contained 3.0 mg pulvis C respectively;
Remarks explanation: the amount that every mouse has the food compositions of hypoxia tolerance is about 10 times of human body recommended amounts.
In 7 days, the feed of freely drinking water of the mouse of above-mentioned experimental group.
(3) the resistance to anoxic experiment of normal pressure is according to improving the resistance to anoxia tolerant function method of inspection in " functional food check and assessment technique standard " (2003 editions) function assessment evaluation test method, experiment mice by above-mentioned gavage after 7 days is put into the 250 mL wide-mouth bottles that 10 g soda limes are housed one by one, bottle cap is smeared vaseline sealing around, make it air tight, timing immediately after adding a cover; Take breath stopped as standard, the beginning and ending time is index, records mouse because of the dead time of anoxic.
(4) result: experimental session, the mouse in each group is all without abnormal response, and result shows, and pulvis A, pulvis B and pulvis C all can significantly improve the resistance to the survival time under hypoxic condition of ICR mouse normal pressure.The order of the raising hypoxia-bearing capability of the functional food composition of three kinds of hypoxia tolerances is followed successively by pulvis A, pulvis C and pulvis B.Explanation thus, the Yuan root freeze-dried powder (the full powder of Yuan root) that vacuum freeze-drying method obtains has retained the anoxia tolerance function composition in Yuan root preferably.Specifically in table 1.
The respectively comparison of the resistance to anoxic life span of group mouse normal pressure of table 1 (
)
Group |
N (number of elements) |
Time-to-live * (min) |
Normal group |
15 |
8.97±1.01?
a |
Pulvis A group |
15 |
12.67±1.02?
d |
Pulvis B group |
15 |
10.16±1.12?
b?
|
Pulvis C group |
15 |
11.57±1.33?
c |
* different letter representation significant differences, P<0.05.
Experiment 2: in order to prove that functional food composition of the present invention has the effect that improves human body hypoxia tolerance, inventor has carried out following experiment:
Choose the age each two of man, female experimenter between 20-30 year, be divided at random I, II group (every group of 1 male 1 female, grouping is in table 2).In the test of continuous 14 d, every day timing take embodiment 2-3 preparation and the food compositions with hypoxia tolerance (being designated hereinafter simply as pulvis A), I group taking dose is 0.5 g/ days, II group taking dose is 1.0 g/ days.Within the 14th day, carry out hypoxemia reaction test respectively at (the 0th day) before taking and after taking.
Hypoxemia reaction test grouping situation is shown in following table 2.
The reaction test of table 2 hypoxemia
Hypoxemia reaction test concrete grammar is: experimenter sucks nitrogen oxygen mixed gas, simulation 5000 m or bis-height above sea levels of 7500 m of being produced by the low oxygen mixing instrument of DY-84 type, and oxygen content is respectively 10.5 % or 7.1 %, and gas flux is 15 L/min; Meanwhile, experimenter carries out the operation of mirror writing numeral, observes the various physiological reactions of experimenter, is measured the transcutaneous oxygen saturation (SpO at the unknown nail folds in a garment circulation of left hand position by bedside monitor
2) and heart rate (HR).Before test starts, experimenter's rest of sitting quietly, makes HR and SpO
2etc. physical signs in stable state (SpO
2>=97 %), signal on-test; Experimenter brings breathing mask, starts to suck low-oxygen gas, presses immediately manual time-keeping, records HR and SpO every 10 s
2; Experimenter on request on record sheet mirror writing digital direct stop test when there is obvious mistake or hypoxemia reaction symptom (dizziness, alliteration, feel sick, be in a cold sweat etc.), if invariably tolerate main suit, as experimenter SpO
2value drops to 60 %, takes rapidly face shield, termination test.Because of body SpO
2in the time of 66-60 %, can lose suddenly consciousness, therefore by SpO
2drop to 60 % and be made as lower limit, termination test at once.By SpO
2being down to 60 % timing definition used from initial value is hypoxemia tolerance time (hypoxia tolerance time, ht-T).
Participate in 4 experimenters of trial test, SpO in hypoxemia reaction test
2variation as shown in Figure 1.Along with breathing the increase of hypoxemia time, human body SpO
2value reduces gradually, and in body, oxygen content is lower, SpO
2value declines faster, until body can adapt to low-oxygen environment in compensatory ground.Under the hypoxia condition of simulation 5000m height above sea level, initial stage experimenter's SpO
2decline very fast, after body can compensatory adaptation low-oxygen environment, SpO
2tend towards stability, as take before composite capsule F
1be stabilized in (80 ± 1) %, M
2higher, reach (90 ± 1) %.Take continuously after pulvis A14 d F
1and M
2spO while reaching compensatory adaptation low-oxygen environment
2mean value increases before taking, and increase rate is respectively 2.5 % and 5.5 %.Under the hypoxia condition of simulation 7500 m height above sea levels, experimenter's SpO
2straight line drops to below 60 %, do not occur compensatory adaptation low-oxygen environment plateau (as simulated under 5000 m height above sea level hypoxia conditions, F
1be stabilized in 80% ± 1%).Relatively the variation of experimenter ht-T under simulation 7500 m height above sea level low-oxygen environments, takes after pulvis A14 d F
2and M
1ht-T extend respectively 41.1%(from 170s to 240s) and 57.1 %(from 140 s to 220 s).
Result shows, functional food composition of the present invention has the human body of raising hypoxia tolerance.
Comparative example 1,0.8kg Yuan root freeze-dried powder, 0.15kg vitamin C and 0.05 kg vitamin(e) C palmitate are evenly mixed, obtain food compositions.
Comparative example 2,0.65kg Yuan root freeze-dried powder, 0.15kg bamboo-leaves flavones (BLF-d24), 0.15kg vitamin C and 0.05 kg vitamin(e) C palmitate evenly mix; Obtain food compositions.
Comparative example 3,0.45kg Yuan root freeze-dried powder, 0.35kg bamboo-leaves flavones (BLF-d24), 0.15 kg vitamin C and 0.05 kg vitamin(e) C palmitate evenly mix; Obtain food compositions.
Comparative example 4,0.55kg Yuan root freeze-dried powder, 0.25kg bamboo-leaves flavones (BLF-d24), 0.05kg vitamin C and 0.15kg vitamin(e) C palmitate evenly mix; Obtain food compositions.
Comparative example 5,0.55 kg Yuan root freeze-dried powder, 0.25 kg bamboo-leaves flavones (BLF-d24), 0.20 kg vitamin C evenly mix; Obtain food compositions.
Comparative example 6,0.55 kg Yuan root freeze-dried powder, 0.25 kg bamboo-leaves flavones (BLF-d24), 0.20 kg vitamin(e) C palmitate evenly mix; Obtain food compositions.
The food compositions of above-mentioned comparative example 1 ~ comparative example 6 gained is checked according to the method for experiment 1, and acquired results is as shown in table 3.
Table 3, respectively organize the resistance to anoxic life span of mouse normal pressure comparison (
)
Group |
N (number of elements) |
Time-to-live * (min) |
Normal group |
15 |
8.97±1.01
a |
Comparative example 1 |
15 |
9.30±0.97
a |
Comparative example 2 |
15 |
10.17±0.91
b |
Comparative example 3 |
15 |
11.11±1.04
c |
Comparative example 4 |
15 |
11.32±1.16
c |
Comparative example 5 |
15 |
11.61±1.21
c |
Comparative example 6 |
15 |
10.86±1.05
c |
* different letter representation significant differences, P<0.05.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.