CN103080316B - 突变δ9延伸酶和它们在制备多不饱和脂肪酸中的用途 - Google Patents

突变δ9延伸酶和它们在制备多不饱和脂肪酸中的用途 Download PDF

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CN103080316B
CN103080316B CN201180040811.9A CN201180040811A CN103080316B CN 103080316 B CN103080316 B CN 103080316B CN 201180040811 A CN201180040811 A CN 201180040811A CN 103080316 B CN103080316 B CN 103080316B
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seq
acid
egd9es
elongase
mutant
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CN103080316A (zh
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M.W.博斯蒂克
H.何
Y.李
Q.朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201180040811.9A 2010-08-26 2011-08-26 突变δ9延伸酶和它们在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN103080316B (zh)

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US37724810P 2010-08-26 2010-08-26
US61/377248 2010-08-26
US61/377,248 2010-08-26
PCT/US2011/049361 WO2012027676A1 (en) 2010-08-26 2011-08-26 Mutant delta-9 elongases and their use in making polyunsaturated fatty acids

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CN103080316A CN103080316A (zh) 2013-05-01
CN103080316B true CN103080316B (zh) 2016-08-31

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US (1) US8980589B2 (enExample)
EP (1) EP2609201B1 (enExample)
JP (1) JP5963749B2 (enExample)
KR (1) KR20140008295A (enExample)
CN (1) CN103080316B (enExample)
AU (1) AU2011293167B2 (enExample)
BR (1) BR112013004356A2 (enExample)
CA (1) CA2808000A1 (enExample)
CL (1) CL2013000507A1 (enExample)
DK (1) DK2609201T3 (enExample)
WO (1) WO2012027676A1 (enExample)

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US8822497B2 (en) * 2007-03-01 2014-09-02 Novartis Ag PIM kinase inhibitors and methods of their use
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
JP2016502851A (ja) 2012-12-21 2016-02-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 微生物細胞中における脂質生成を改変するためのｓｏｕ２ソルビトール利用タンパク質をコードするポリヌクレオチドの下方制御
CN110358692B (zh) * 2018-04-09 2021-07-27 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
KR102085555B1 (ko) * 2018-08-27 2020-03-06 대한민국(농촌진흥청장) 아라키돈산 생합성을 위한 신규 델타-5 불포화효소 및 이의 용도

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CN101765658A (zh) * 2007-04-03 2010-06-30 纳幕尔杜邦公司 复合酶及其在制备多不饱和脂肪酸中的用途
CN101848996A (zh) * 2007-04-16 2010-09-29 纳幕尔杜邦公司 △9延伸酶及其在制备多不饱和脂肪酸中的用途

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AU2011293167A1 (en) 2013-01-24
CA2808000A1 (en) 2012-03-01
JP5963749B2 (ja) 2016-08-03
AU2011293167B2 (en) 2016-12-08
KR20140008295A (ko) 2014-01-21
BR112013004356A2 (pt) 2017-06-27
US8980589B2 (en) 2015-03-17
WO2012027676A1 (en) 2012-03-01
EP2609201B1 (en) 2016-03-02
US20120226062A1 (en) 2012-09-06
EP2609201A1 (en) 2013-07-03
CL2013000507A1 (es) 2014-03-28
CN103080316A (zh) 2013-05-01
DK2609201T3 (en) 2016-06-06
JP2013541328A (ja) 2013-11-14

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