CN103013179A - Method for enhancing extinction ratio of capsanthin pigment - Google Patents
Method for enhancing extinction ratio of capsanthin pigment Download PDFInfo
- Publication number
- CN103013179A CN103013179A CN2013100097712A CN201310009771A CN103013179A CN 103013179 A CN103013179 A CN 103013179A CN 2013100097712 A CN2013100097712 A CN 2013100097712A CN 201310009771 A CN201310009771 A CN 201310009771A CN 103013179 A CN103013179 A CN 103013179A
- Authority
- CN
- China
- Prior art keywords
- capsanthin
- absorption ratio
- washed
- intermediate liquid
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses a method for enhancing extinction ratio of a capsanthin pigment, which comprises the following steps: degumming: washing a capsanthin pigment stock solution with a water-petroleum ether mixed solvent to obtain a washed capsanthin pigment A and an intermediate solution; and alcohol washing: rewashing the washed capsanthin pigment A with anhydrous alcohol to obtain a high-extinction-ratio capsanthin pigment, rewashing the intermediate solution to obtain a low-extinction-ratio capsanthin pigment, centrifuging the intermediate solution obtained by rewashing the intermediate solution by using a centrifugal machine to obtain a sample C, and drying C which is the byproduct. The invention has the following advantages: (1) the extinction ratio of the product is effectively enhanced to 1.0024, exceeding 1.0000; (2) the cost is low since all the organic solvents are common; and (3) the technique is simple and feasible for operation, and is beneficial to large-scale production.
Description
Technical field
The present invention relates to a kind of production method of capsanthin, especially a kind of method that improves the capsanthin absorption ratio.
Background technology
After the capsanthin absorption ratio refers to that capsanthin is with acetone solution, the ratio of absorbancy under 470nm and 454nm respectively, directly reflected the ratio of red composition (Capsorubin, capsorubin) and yellow composition (zeaxanthin, β-carotene) in the capsanthin, this index affects the quality of capsanthin product to a great extent.
At present, the absorption ratio of capsanthin all is lower than 1.0000.People are tending towards refinement to the research of capsanthin, and the client is also more and more stricter to the requirement of product, in order to satisfy the client to the requirement of this index of absorption ratio, therefore, study a kind of method that improves the capsanthin absorption ratio, are the present technical issues that need to address.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method that improves the capsanthin absorption ratio.
The technical scheme that the present invention solves its technical problem is:
A kind of method that improves the capsanthin absorption ratio, comprise come unstuck step and alcohol wash two steps, the described step of coming unstuck is about to the mixed solvent of capsanthin stoste water and sherwood oil composition and washes, namely get and wash rear capsanthin A and intermediate liquid, described alcohol wash namely will be washed rear capsanthin A with dehydrated alcohol and be washed, high absorption ratio capsanthin namely, intermediate liquid is washed to get low absorption ratio capsanthin again, interstitial fluid carried out centrifugal treating with whizzer and gets sample C among intermediate liquid was washed again, with the C oven dry, C is byproduct.
Described washing namely got a certain amount of capsanthin stoste and is positioned in the container, according to capsanthin stoste: the mixed solvent that the volume ratio of water: sherwood oil=1:1:2 forms water and sherwood oil mixes with capsanthin and stirs, then place separating funnel to leave standstill some minutes, lower layer of water is bled off, again upper strata liquid is separated with intermediate liquid, at last with upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition wash after capsanthin A; Described intermediate liquid is washed again, be about to intermediate liquid by intermediate liquid: the volume ratio of water: sherwood oil=1:1:2 mixes the mixed solvent of water and sherwood oil composition and stirs with intermediate liquid, place separating funnel to leave standstill some minutes, repeat again aforesaid operations once, with rear twice upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition and merge to get sample B, B is inferior grade absorption ratio capsanthin, rear twice intermediate liquid merging is carried out centrifugal treating with whizzer get sample C, with the C oven dry, C is byproduct.
Described alcohol wash, namely will wash rear capsanthin A according to the volume ratio of washing rear capsanthin A: dehydrated alcohol=1:3 mixes with dehydrated alcohol and stirs, upper strata liquid is separated with subnatant, subnatant is heavily being washed as stated above, wash altogether 4 times, above-mentioned 4 times upper strata liquid is concentrated respectively to get A1, A2, A3 and A4, at last subnatant is concentrated to get sample D, D is high absorption ratio capsanthin, and A1, A2, A3 and A4 are respectively the capsanthin of different grades absorption ratio.
The centrifugal condition of described sample C is 30 ℃ of 4000r, 10 minutes, bake out temperature.
Described time of repose is one hour.
Advantage of the present invention is:
(1) effectively improves the absorption ratio of product, make absorption ratio reach 1.0024, surpassed 1.0000.
(2) cost is low, use all be the organic solvent of commonly using.
(3) technique is simple, and operation feasible is conducive to scale operation.
Embodiment
A kind of method that improves the capsanthin absorption ratio, comprise come unstuck step and alcohol wash two steps, the described step of coming unstuck is about to the mixed solvent of capsanthin stoste water and sherwood oil composition and washes, namely get and wash rear capsanthin A and intermediate liquid, described alcohol wash namely will be washed rear capsanthin A with dehydrated alcohol and be washed, high absorption ratio capsanthin namely, intermediate liquid is washed to get low absorption ratio capsanthin again, interstitial fluid carried out centrifugal treating with whizzer and gets sample C among intermediate liquid was washed again, with the C oven dry, C is byproduct.
Described washing namely got a certain amount of capsanthin stoste and is positioned in the container, according to capsanthin stoste: the mixed solvent that the volume ratio of water: sherwood oil=1:1:2 forms water and sherwood oil mixes with capsanthin and stirs, then place separating funnel to leave standstill some minutes, lower layer of water is bled off, again upper strata liquid is separated with intermediate liquid, at last with upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition wash after capsanthin A; Described intermediate liquid is washed again, be about to intermediate liquid by intermediate liquid: the volume ratio of water: sherwood oil=1:1:2 mixes the mixed solvent of water and sherwood oil composition and stirs with intermediate liquid, place separating funnel to leave standstill one hour, repeat again aforesaid operations once, with rear twice upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition and merge to get sample B, B is inferior grade absorption ratio capsanthin, with rear twice intermediate liquid merge with whizzer centrifugal treating under the 4000r condition 10 minutes, under 30 ℃ of temperature condition with the C oven dry, C is byproduct.Described alcohol wash, namely will wash rear capsanthin A according to the volume ratio of washing rear capsanthin A: dehydrated alcohol=1:3 mixes with dehydrated alcohol and stirs, upper strata liquid is separated with subnatant, subnatant is heavily being washed as stated above, wash altogether 4 times, above-mentioned 4 times upper strata liquid is concentrated respectively to get A1, A2, A3 and A4, at last subnatant is concentrated to get sample D, D is high absorption ratio capsanthin, and A1, A2, A3 and A4 are respectively the capsanthin of different grades absorption ratio.
Embodiment:
1, comes unstuck and get the 1000ml capsanthin, according to pigment: the ratio of water: sherwood oil=1:1:2 is washed pigment, wash three times, upper strata liquid concentrates to get sample A for the first time, rear twice upper strata liquid is concentrated to merge to get sample B, bleeds off water layer, merges intermediate liquid liquid and carries out the centrifugal sample C of getting, oven dry, pending.And record absorption ratio and the look valency of sample A and B.
2, alcohol wash is washed respectively sample A according to the ratio of pigment: dehydrated alcohol=1:3, washes four times, and then each upper strata liquid concentrates to get A1 A2 A3 A4, the concentrated sample D that to get of subnatant.
Annotate: 40 ℃-0.09MP of above-mentioned rotary evaporation condition.
Time of repose is one hour.
Whizzer condition: 4000r 10 minutes.
Claims (5)
1. method that improves the capsanthin absorption ratio, it is characterized in that, comprise come unstuck step and alcohol wash two steps, the described step of coming unstuck is about to the mixed solvent of capsanthin stoste water and sherwood oil composition and washes, namely get and wash rear capsanthin A and intermediate liquid, described alcohol wash namely will be washed rear capsanthin A with dehydrated alcohol and be washed, high absorption ratio capsanthin namely, intermediate liquid is washed to get low absorption ratio capsanthin again, interstitial fluid carried out centrifugal treating with whizzer and gets sample C among intermediate liquid was washed again, with the C oven dry, C is byproduct.
2. the method for raising capsanthin absorption ratio according to claim 1, it is characterized in that, described washing namely got a certain amount of capsanthin stoste and is positioned in the container, according to capsanthin stoste: the mixed solvent that the volume ratio of water: sherwood oil=1:1:2 forms water and sherwood oil mixes with capsanthin and stirs, then place separating funnel to leave standstill some minutes, lower layer of water is bled off, again upper strata liquid is separated with intermediate liquid, at last with upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition wash after capsanthin A; Described intermediate liquid is washed again, be about to intermediate liquid by intermediate liquid: the volume ratio of water: sherwood oil=1:1:2 mixes the mixed solvent of water and sherwood oil composition and stirs with intermediate liquid, place separating funnel to leave standstill some minutes, repeat again aforesaid operations once, with rear twice upper strata liquid with rotatory evaporator 40 ℃ ,-concentrate under the 0.09MP condition and merge to get sample B, B is inferior grade absorption ratio capsanthin, rear twice intermediate liquid merging is carried out centrifugal treating with whizzer get sample C, with the C oven dry, C is byproduct.
3. the method for raising capsanthin absorption ratio according to claim 1, it is characterized in that, described alcohol wash, namely will wash rear capsanthin A according to the volume ratio of washing rear capsanthin A: dehydrated alcohol=1:3 mixes with dehydrated alcohol and stirs, upper strata liquid is separated with subnatant, subnatant is heavily being washed as stated above, wash altogether 4 times, above-mentioned 4 times upper strata liquid is concentrated respectively to get A1, A2, A3 and A4, at last subnatant is concentrated to get sample D, D is high absorption ratio capsanthin, A1, A2, A3 and A4 are respectively the capsanthin of different grades absorption ratio.
4. the method for raising capsanthin absorption ratio according to claim 2 is characterized in that, the centrifugal condition of sample C is 30 ℃ of 4000r, 10 minutes, bake out temperature.
5. the method for raising capsanthin absorption ratio according to claim 2 is characterized in that, time of repose is one hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100097712A CN103013179A (en) | 2013-01-11 | 2013-01-11 | Method for enhancing extinction ratio of capsanthin pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100097712A CN103013179A (en) | 2013-01-11 | 2013-01-11 | Method for enhancing extinction ratio of capsanthin pigment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103013179A true CN103013179A (en) | 2013-04-03 |
Family
ID=47962309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100097712A Pending CN103013179A (en) | 2013-01-11 | 2013-01-11 | Method for enhancing extinction ratio of capsanthin pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103013179A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305028A (en) * | 2013-06-20 | 2013-09-18 | 邯郸市中进天然色素有限公司 | Method for improving color value of capsanthin |
| CN107523090A (en) * | 2017-06-29 | 2017-12-29 | 河北邯兆生物科技有限公司 | A kind of method for improving, improving capsicum red pigment absorption ratio |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080089716A (en) * | 2007-04-02 | 2008-10-08 | (주)원텍 | Extraction of pigment material and capsaicin from a kind of red pepper using a supercritical carbon dioxide |
| CN101760039A (en) * | 2009-09-26 | 2010-06-30 | 美科尔(河北)生物科技有限公司 | Method for removing capsaicin during production process of capsicum red pigment |
| WO2011135519A1 (en) * | 2010-04-29 | 2011-11-03 | Synthite Industries Ltd. | A capsicum annuum extract and process thereof |
| CN102492307A (en) * | 2011-12-08 | 2012-06-13 | 晨光生物科技集团股份有限公司 | Hydration degumming method for hot pepper extract |
| CN102604417A (en) * | 2011-12-15 | 2012-07-25 | 武城县英潮经贸有限公司 | New technology for separating haematochrome and pectin from capsanthin centrifugate |
| CN102807770A (en) * | 2012-07-30 | 2012-12-05 | 江苏科技大学 | Method for preparing paprika red pigment by ultrasonic pre-treatment |
-
2013
- 2013-01-11 CN CN2013100097712A patent/CN103013179A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080089716A (en) * | 2007-04-02 | 2008-10-08 | (주)원텍 | Extraction of pigment material and capsaicin from a kind of red pepper using a supercritical carbon dioxide |
| CN101760039A (en) * | 2009-09-26 | 2010-06-30 | 美科尔(河北)生物科技有限公司 | Method for removing capsaicin during production process of capsicum red pigment |
| WO2011135519A1 (en) * | 2010-04-29 | 2011-11-03 | Synthite Industries Ltd. | A capsicum annuum extract and process thereof |
| CN102492307A (en) * | 2011-12-08 | 2012-06-13 | 晨光生物科技集团股份有限公司 | Hydration degumming method for hot pepper extract |
| CN102604417A (en) * | 2011-12-15 | 2012-07-25 | 武城县英潮经贸有限公司 | New technology for separating haematochrome and pectin from capsanthin centrifugate |
| CN102807770A (en) * | 2012-07-30 | 2012-12-05 | 江苏科技大学 | Method for preparing paprika red pigment by ultrasonic pre-treatment |
Non-Patent Citations (1)
| Title |
|---|
| 乔世伟: "辣椒红色素提取工艺研究", 《山西食品工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305028A (en) * | 2013-06-20 | 2013-09-18 | 邯郸市中进天然色素有限公司 | Method for improving color value of capsanthin |
| CN107523090A (en) * | 2017-06-29 | 2017-12-29 | 河北邯兆生物科技有限公司 | A kind of method for improving, improving capsicum red pigment absorption ratio |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jung et al. | Comparison of lignin, cellulose, and hemicellulose contents for biofuels utilization among 4 types of lignocellulosic crops | |
| CN102621029A (en) | Measurement method of soft asphalt quinoline insoluble substance | |
| CN103030516B (en) | Purification method of coking xylene | |
| CN103013179A (en) | Method for enhancing extinction ratio of capsanthin pigment | |
| CN103965950A (en) | Method for separating phenols in coal liquefaction oil | |
| CN103898785A (en) | Comprehensive utilization method of biomass raw material | |
| CN102276638A (en) | Process for producing boron trifluoride complex | |
| CN102399302B (en) | A kind of ultrasonic extracting method of pectin | |
| CN104448923A (en) | Process for enhancing extinction ratio of capsanthin pigment | |
| BR112015020808A2 (en) | process of producing alcohols and / or solvents from lignocellulosic biomass by washing the solid residue obtained after fermentation | |
| CN104862997A (en) | High-temperature-resistant textile dye | |
| EA201500606A1 (en) | METHOD FOR ISOLATION OF LEVULINIC ACID AND FORMIC ACID | |
| CN104448902A (en) | Grape skin pigment extraction technology | |
| CN103602520A (en) | Method for reducing color of soybean oil by using synergistic effect of monochromatic light and attapulgite | |
| CN102603916B (en) | Refining method of (1-3)-beta-D-glucan | |
| CN102464898B (en) | Method for recovering yellow ginger pigment from yellow ginger hydrolysate saponin extraction waste liquid | |
| CN103572382A (en) | Deoiling and degumming treatment method for waste bave | |
| CN103864868A (en) | Green extraction process of natural active flavone-rutin | |
| AR096349A1 (en) | PROCEDURE FOR EXTRACTION OF FERULIC ACID WITH PRE-TREATMENT | |
| CN104710544A (en) | Method for extracting pectin from tobacco waste by using ultrasonic and organic acid | |
| CN105038848A (en) | Pretreatment method of biomass pyrolysis tar | |
| CN103665933A (en) | Disperse dye | |
| CN103485158A (en) | Bleaching and dyeing process of cotton warp linen | |
| CN204462068U (en) | The oil determination instrument of novel crude oil exploitation | |
| RU2013136998A (en) | METHOD FOR PREPARING PROTEIN-CARBOHYDRATE PRODUCTS BASED ON SOY |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130403 |