CN107523090A - A kind of method for improving, improving capsicum red pigment absorption ratio - Google Patents
A kind of method for improving, improving capsicum red pigment absorption ratio Download PDFInfo
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- CN107523090A CN107523090A CN201710515538.XA CN201710515538A CN107523090A CN 107523090 A CN107523090 A CN 107523090A CN 201710515538 A CN201710515538 A CN 201710515538A CN 107523090 A CN107523090 A CN 107523090A
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- red pigment
- capsicum red
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- ethyl acetate
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of method for improving, improving capsicum red pigment absorption ratio, the present invention includes degumming removal of impurities and the step of liquid-liquid extraction two, the degumming step, capsicum red pigment stoste is stirred with water and ethyl acetate mixture, stand, then by centrifuging and being concentrated in vacuo capsicum red pigment A and middle interstitial fluid after producing degumming；The liquid-liquid extraction extracts degumming capsicum red pigment A ethanol, ethyl acetate mixture, isolated lower floor's mixed liquor, and concentration is de- residual to obtain the capsicum red pigment of high absorption ratio.The preparation method of the present invention is simple to operate, change the polarity of mixed solution by adjusting the content of ethyl acetate in extract in concrete operations, utilize red component in capsicum red pigment and yellow color component in the mixed solvent solubility or the difference of distribution coefficient, yellow color component in capsicum red pigment is set to be dissolved in the mixed solvent, red component is separated from the mixed solvent.So as to obtain the capsicum red pigment that absorption ratio is more than 1.0000.
Description
Technical field
The present invention relates to a kind of method for improving, improving capsicum red pigment absorption ratio.
Background technology
Capsicum red pigment, also known as capsanthin, capsicum oleoresin, are the natural colorants extracted from capsicum, and capsicum shows
Color substance is mainly capsicum red pigment, and capsicum red pigment belongs to carotenoidses pigment, the main red larger including polarity
Component and the less yellow color component of polarity, red component are capsorubin and capsorubin, and the component of yellow is mainly Hu trailing plants
Bu Su and zeaxanthin.Wherein the main coloring components of capsicum red pigment are capsorubin and capsorubin.Capsicum flood pigment
Lovely luster, there is significant effect in terms of tinting strength, tinting power and Bao Se, it can be used not only for the safety coloring of varieties of food items,
But also there are certain nutrition health-care functions.
It is visible in 470nm and 454nm respectively after capsicum red pigment absorption ratio refers to capsicum red pigment acetone solution
The ratio of absorbance under light irradiation, absorption ratio can be used for reflecting the ratio of haematochrome component and yellow component in capsicum red pigment.
Extinction ratio is bigger, and red component content is higher, and the color that capsicum red pigment shows is closer to red；Extinction ratio is got over
Small, yellow color component content is higher, and the color that capsicum red pigment shows is closer to yellow.In addition, absorption ratio is largely
On can also reflect the quality of capsicum red pigment, for capsicum red pigment, its purity is higher, the Huang of the low absorption ratio mixed
Color colour component and capsorubin are fewer, and its absorption ratio is also higher.
With the improvement of living standards, people gradually have higher requirement to capsanthin product, people are to capsanthin
Research also tends to refine, and requirement of the client to product is also more and more stricter.(absorption ratio is less than common capsicum red pigment
1.000) can not meet the needs of people, the capsicum red pigment of high absorption ratio is because its color is vivider, and colorability is more
By force, utilization rate height is increasingly favored by people.
Preparing the capsicum red pigment of high absorption ratio at present has column chromatography, and for column chromatography because its is cumbersome, working condition will
Ask higher, and yield is smaller, can not realize industrialized production.Therefore the present invention, being capable of industrialized production in line with simple to operate
Purpose, develop a kind of method that organic solvent method prepares the capsicum red pigment of high absorption ratio.
The content of the invention
The technical problems to be solved by the invention be to provide it is a kind of prepare high-purity, suitable for large-scale production improvement,
The method for improving capsicum red pigment absorption ratio.
In order to solve the above technical problems, the technical solution used in the present invention is：One kind improves, improves capsicum red pigment
The method of absorption ratio, it is characterised in that：Including degumming removal of impurities and the step of liquid-liquid extraction two, the degumming step, i.e., by capsanthin
Pigment stoste is stirred with water and ethyl acetate mixture, is stood, then after centrifuging and vacuum concentration produces degumming
Capsicum red pigment A and middle interstitial fluid；The liquid-liquid extraction mixes degumming capsicum red pigment A ethanol, ethyl acetate molten
Liquid extracts, isolated lower floor's mixed liquor, and concentration is de- residual to obtain the capsicum red pigment of high absorption ratio.
Preferably, described degumming takes a certain amount of capsicum red pigment stoste to be positioned in container, red according to capsicum
Element：Water：Ethyl acetate is 1:2:The mixed liquor that water and ethyl acetate form is mixed and stirred by 3 volume ratio with capsicum red pigment
Mix uniformly, temperature is then risen to 45 DEG C, 45 DEG C of constant temperature stirs 0.5 hour, then stands 2 hours；Lower floor's water is bled off, then
Upper liquid and middle interstitial fluid are centrifuged, obtain centrifugation mixed liquor；The upper strata mixed liquor of mixed liquor will finally be centrifuged in 40-45
DEG C, be concentrated under reduced pressure under the conditions of-0.07-0.1MPa, it is capsicum red pigment A to obtain concentrate；Centrifuge lower floor's mixing of mixed liquor
Liquid obtains pigment mixing liquid B and pectic substance C, pigment mixing liquid B with centrifuge 4000r, 10 minutes centrifugal treatings
It is de- it is residual obtain pigment D, pectic substance C dries to obtain accessory substance.
Preferably, described solvent extraction, i.e., according to capsicum red pigment A：Ethanol, ethyl acetate mixture=1:2
Ratio carries out hybrid extraction, stirring at normal temperature 0.5 hour, stands 2 hours, and lower floor's solution is concentrated, and obtains capsicum red pigment E；
Ethanol, ethyl acetate mixture are ethanol：Ethyl acetate=95：5.
It is using beneficial effect caused by above-mentioned technical proposal：The preparation method of the present invention is simple to operate, used molten
Agent is Conventional solvents ethanol and ethyl acetate, and non-selected waving property of height acetone.Because acetone is volatile, excitant is stronger, solvent
The rate of recovery is relatively low, and easy corrosion pipeline.It is mixed so as to change by adjusting the content of ethyl acetate in extract in concrete operations
The polarity of solution is closed, utilizes red component in capsicum red pigment and yellow color component in the mixed solvent solubility or distribution coefficient
Difference, yellow color component in capsicum red pigment is set to be dissolved in the mixed solvent, red component is separated from the mixed solvent.From
And obtain the capsicum red pigment that absorption ratio is more than 1.0000.
The present invention can also prepare that purity is higher, absorption ratio while the capsicum red pigment of high absorption ratio is prepared
Yellow color component pigment in relatively low capsicum red pigment, the overall utilization rate of capsicum red pigment is improved, it is green, save
Resource.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described implementation
Example is only the part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, this area
The every other embodiment that those of ordinary skill is obtained under the premise of creative work is not made, belongs to guarantor of the present invention
The scope of shield.
Many details are elaborated in the following description to facilitate a thorough understanding of the present invention, still the present invention may be used also
Implemented with being different from other manner described here using other, those skilled in the art can be without prejudice in the present invention
Similar popularization is done in the case of culvert, therefore the present invention is not limited by following public specific embodiment.
Disclosure sets forth a kind of method for improving, improving capsicum red pigment absorption ratio, it includes degumming removal of impurities and liquid liquid
Two steps are extracted, described degumming takes a certain amount of capsicum red pigment stoste to be positioned in container, according to capsicum red pigment：
Water：Ethyl acetate is 1:2:3 volume ratio mixes the mixed liquor that water and ethyl acetate form with capsicum red pigment equal
It is even, temperature is then risen to 45 DEG C, 45 DEG C of constant temperature stirs 0.5 hour, then stands 2 hours；Lower floor's water is bled off, then will be upper
Layer liquid and middle interstitial fluid centrifuge, and obtain centrifugation mixed liquor；Finally by centrifuge mixed liquor upper strata mixed liquor 40-45 DEG C ,-
It is concentrated under reduced pressure under the conditions of 0.07-0.1MPa, it is capsicum red pigment A to obtain concentrate；The lower floor's mixed liquor for centrifuging mixed liquor is used
Centrifuge 4000r, 10 minutes centrifugal treatings obtain pigment mixing liquid B and pectic substance C, and pigment mixing liquid B takes off residual obtain
Dry to obtain accessory substance to pigment D, pectic substance C.
Described solvent extraction, i.e., according to capsicum red pigment A：Ethanol, ethyl acetate mixture=1:2 ratio is entered
Row hybrid extraction, stirring at normal temperature 0.5 hour, 2 hours are stood, and lower floor's solution is concentrated, obtain capsicum red pigment E；Ethanol,
Ethyl acetate mixture is ethanol：Ethyl acetate=95：5.
Embodiment 1
1. degumming takes 200kg capsicum red pigments, color value 82.54, absorption ratio is： 0.9856.According to pigment：Water：Acetic acid
Ethyl ester=1:2:3 ratio is added in extraction kettle, and mixed liquor is stirred 10 minutes, makes its mixing, then mixed liquor heats up
To 45 DEG C, 45 DEG C of constant temperature stirs 0.5 hour, makes it well mixed.Then mixed liquor is stood 2 hours.Lower floor's water is bled off, then
Upper liquid and middle interstitial fluid are centrifuged, obtain centrifugation mixed liquor；Centrifuge mixed liquor upper strata mixed liquor 40-45 DEG C ,-0.07-
Be concentrated under reduced pressure under the conditions of 0.1MPa, obtain concentrate be capsicum red pigment A 135kg, color value 120, absorption ratio 0.9905,
Centrifugation mixed liquor lower floor's mixed liquor obtains pigment mixing liquid B and pectin class thing with centrifuge 4000r, 10 minutes centrifugal treatings
Matter C, pigment mixing liquid B take off it is residual obtain pigment D, pectic substance dries to obtain accessory substance.
2. solvent extraction is placed in above-mentioned capsicum red pigment A135kg in extraction kettle, while 13.5kg second is added into kettle
Acetoacetic ester and 256.5kg ethanol.Stirring at normal temperature 1 hour, stand 2 hours.Mixed liquor is separated, and levels solution difference is dense
Contracting, obtains lower floor capsicum red pigment B, color value 131.7, absorption ratio 1.0024, quality 70kg.Upper strata capsicum red pigment
C, color value 115.5, quality 60kg, absorption ratio 0.9654.
3. by the production method with the present invention, the absorption ratio of capsicum red pigment is risen to by the 0.9856 of initiation material
1.0024, capsanthin yield of the absorption ratio more than 1.000 is 55.85%, and the total color value rate of recovery of capsicum red pigment is 97.82%.
Embodiment 2
1. degumming takes 200kg capsicum red pigments, color value 78.32, absorption ratio is： 0.9815.According to pigment：Water：Acetic acid
Ethyl ester=1:2:3 ratio is added in extraction kettle, and mixed liquor is stirred 10 minutes, makes its mixing, then mixed liquor heats up
To 45 DEG C, 45 DEG C of constant temperature stirs 0.5 hour, makes it well mixed.Then mixed liquor is stood 2 hours.Lower floor's water is bled off, then
Upper liquid and middle interstitial fluid are centrifuged, obtain centrifugation mixed liquor, centrifugation mixed liquor upper strata mixed liquor 40-45 DEG C ,-0.07-
Be concentrated under reduced pressure under the conditions of 0.1MPa, obtain concentrate be capsicum red pigment A 130kg, color value 115, absorption ratio 0.9910,
Centrifugation mixed liquor lower floor's mixed liquor obtains pigment mixing liquid B and pectin class thing with centrifuge 4000r, 10 minutes centrifugal treatings
Matter C, pigment mixing liquid B take off it is residual obtain pigment D, pectic substance dries to obtain accessory substance.
2. solvent extraction is placed in above-mentioned capsicum red pigment A130kg in extraction kettle, while 13kg acetic acid is added into kettle
Ethyl ester and 247kg ethanol.Stirring at normal temperature 1 hour, stand 2 hours.Mixed liquor is separated, and levels solution is concentrated respectively, is obtained
To lower floor capsicum red pigment B, color value 156.64, absorption ratio 1.0015, quality 60kg.Upper strata capsicum red pigment C, color
Valency is 105.5, quality 5.39kg, absorption ratio 0.9532.
3. by the production method with the present invention, the absorption ratio of capsicum red pigment is risen to by the 0.9815 of initiation material
1.0015, capsanthin yield of the absorption ratio more than 1.000 is 60.00%, and the total color value rate of recovery of capsicum red pigment is 96.30%.
Claims (3)
- A kind of 1. method for improving, improving capsicum red pigment absorption ratio, it is characterised in that：Including degumming removal of impurities and liquid-liquid extraction two Step, the degumming step, i.e., capsicum red pigment stoste is stirred with water and ethyl acetate mixture, stood, then By centrifuging and being concentrated in vacuo capsicum red pigment A and middle interstitial fluid after producing degumming；The liquid-liquid extraction is i.e. by degumming capsanthin Pigment A ethanol, ethyl acetate mixture extraction, isolated lower floor's mixed liquor, concentration is de- residual to obtain high absorption ratio Capsicum red pigment.
- 2. a kind of method for improving, improving capsicum red pigment absorption ratio according to claim 1, it is characterised in that described Degumming i.e. take a certain amount of capsicum red pigment stoste to be positioned in container, according to capsicum red pigment：Water：Ethyl acetate is 1:2: The mixed liquor that water and ethyl acetate form is mixed evenly 3 volume ratio with capsicum red pigment, then rises to temperature 45 DEG C, 45 DEG C of constant temperature stirs 0.5 hour, then stands 2 hours；Lower floor's water is bled off, then upper liquid and the centrifugation of middle interstitial fluid are divided From obtaining centrifugation mixed liquor；Finally the upper strata mixed liquor for centrifuging mixed liquor is depressurized under the conditions of 40-45 DEG C ,-0.07-0.1MPa Concentration, it is capsicum red pigment A to obtain concentrate；Centrifuge lower floor's mixed liquor centrifuge 4000r of mixed liquor, at centrifugation in 10 minutes Reason obtains pigment mixing liquid B and pectic substance C, pigment mixing liquid B take off it is residual obtain pigment D, pectic substance C is dried To accessory substance.
- A kind of 3. method for improving, improving capsicum red pigment absorption ratio according to claim 1 or 2, it is characterised in that institute The solvent extraction stated, i.e., according to capsicum red pigment A：Ethanol, ethyl acetate mixture=1:2 ratio carries out hybrid extraction, Stirring at normal temperature 0.5 hour, 2 hours are stood, and lower floor's solution is concentrated, obtain capsicum red pigment E；Ethanol, ethyl acetate mixing Solution is ethanol：Ethyl acetate=95：5.
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| CN201710515538.XA CN107523090A (en) | 2017-06-29 | 2017-06-29 | A kind of method for improving, improving capsicum red pigment absorption ratio |
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| CN201710515538.XA CN107523090A (en) | 2017-06-29 | 2017-06-29 | A kind of method for improving, improving capsicum red pigment absorption ratio |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029312A (en) * | 2020-08-20 | 2020-12-04 | 河北东之星生物科技股份有限公司 | Preparation method of capsorubin with high light absorption ratio and capsorubin with high content |
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|---|---|---|---|---|
| CN1392201A (en) * | 2002-07-18 | 2003-01-22 | 中山大学 | Extracting and purifying method for natural capsanthin pigment |
| CN103013179A (en) * | 2013-01-11 | 2013-04-03 | 邯郸市中进天然色素有限公司 | Method for enhancing extinction ratio of capsanthin pigment |
| CN103305028A (en) * | 2013-06-20 | 2013-09-18 | 邯郸市中进天然色素有限公司 | Method for improving color value of capsanthin |
| CN104448923A (en) * | 2013-09-24 | 2015-03-25 | 青岛中人智业生物科技有限公司 | Process for enhancing extinction ratio of capsanthin pigment |
| CN105017055A (en) * | 2015-07-05 | 2015-11-04 | 贵州大学 | Method for rapidly extracting and separating capsanthin and capsaicin from red chillies |
| CN105505225A (en) * | 2015-12-29 | 2016-04-20 | 云南宏绿辣素有限公司 | Method for separating capsaicin and pigment in capsicum oleoresin with high capsaicin content and refining capsaicin |
-
2017
- 2017-06-29 CN CN201710515538.XA patent/CN107523090A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1392201A (en) * | 2002-07-18 | 2003-01-22 | 中山大学 | Extracting and purifying method for natural capsanthin pigment |
| CN103013179A (en) * | 2013-01-11 | 2013-04-03 | 邯郸市中进天然色素有限公司 | Method for enhancing extinction ratio of capsanthin pigment |
| CN103305028A (en) * | 2013-06-20 | 2013-09-18 | 邯郸市中进天然色素有限公司 | Method for improving color value of capsanthin |
| CN104448923A (en) * | 2013-09-24 | 2015-03-25 | 青岛中人智业生物科技有限公司 | Process for enhancing extinction ratio of capsanthin pigment |
| CN105017055A (en) * | 2015-07-05 | 2015-11-04 | 贵州大学 | Method for rapidly extracting and separating capsanthin and capsaicin from red chillies |
| CN105505225A (en) * | 2015-12-29 | 2016-04-20 | 云南宏绿辣素有限公司 | Method for separating capsaicin and pigment in capsicum oleoresin with high capsaicin content and refining capsaicin |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029312A (en) * | 2020-08-20 | 2020-12-04 | 河北东之星生物科技股份有限公司 | Preparation method of capsorubin with high light absorption ratio and capsorubin with high content |
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Application publication date: 20171229 |