CN102987062A - Feed probiotic fermented base material - Google Patents

Feed probiotic fermented base material Download PDF

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Publication number
CN102987062A
CN102987062A CN 201210589745 CN201210589745A CN102987062A CN 102987062 A CN102987062 A CN 102987062A CN 201210589745 CN201210589745 CN 201210589745 CN 201210589745 A CN201210589745 A CN 201210589745A CN 102987062 A CN102987062 A CN 102987062A
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CN
China
Prior art keywords
feed
base material
probiotic fermented
preparation
manioc waste
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Pending
Application number
CN 201210589745
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Chinese (zh)
Inventor
邹步珍
龙泽云
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NANNING BAIJI BIOENGINEERING CO Ltd
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NANNING BAIJI BIOENGINEERING CO Ltd
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Application filed by NANNING BAIJI BIOENGINEERING CO Ltd filed Critical NANNING BAIJI BIOENGINEERING CO Ltd
Priority to CN 201210589745 priority Critical patent/CN102987062A/en
Publication of CN102987062A publication Critical patent/CN102987062A/en
Pending legal-status Critical Current

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Abstract

The invention relates to a feed probiotic fermented base material with manioc waste reducing sugar and a preparation method thereof. The base material takes the manioc waste reducing sugar as the main raw material, composites corn flour, fructo-oligosaccharide, milk powder and yeast extract, and is prepared by fully mixing the raw materials through methods of mixing, stirring and the like. When the feed probiotic fermented base material is used, the feed probiotics, such as lactobacillus lactis, bifidobacterium bifidum, saccharomyces cerevisiae and the like are fully mixed, a moderate amount of water is added, and after the mixture ferments for a period of time, the product can be used for enlarged cultivation of the feed probiotics or serve as the feed probiotic fermented base material. The feed probiotic fermented base material is remarkable in cultivation effect, simple in preparation process, saving in energy and time, and is apt to achieve industrial production.

Description

A kind of feeding probiotics fermention base-material
Technical field
The present invention relates to a kind of additive for microbe feedstuff, especially contain feeding probiotics fermention base-material of manioc waste reduced sugar and preparation method thereof.
Background technology
Probio is the active microorganism that can produce to body beneficial effect when intake is enough.People often call additive for microbe feedstuff to the probio that is applied in the animal husbandry production.Studies show that additive for microbe feedstuff has to be safeguarded that animal intestinal is healthy, improper release stress, improve the animal house environment, regulate the body fat metabolism and improve the function of livestock products quality.Also have the researcher to think that additive for microbe feedstuff has the effect of substitute antibiotics function.。
The research of additive for microbe feedstuff or feeding probiotic bacteria culture medium or fermentation material all there is report in pertinent literature and the patent at home.
1. Liu Yang etc. uses the test of 4 factors, 3 horizontal quadratures and single factor experiment that its culture medium is improved in " property research of a bacillus licheniformis and fermentation medium optimization ", and its fermentation condition is optimized.4 kinds of factors on the conspicuousness order that viable count affects are: MnSO4>NaCl>dusty yeast>peptone, conspicuousness order on the impact of gemma number is: MnSO4>peptone>ferment tank amplifies to be cultivated, the best is put the tank time should be at 14~16h, about 101.63 * 109/ml of thalline quantity that obtains, the gemma rate is 97.11%.(Agriculture of Anhui science-2012 year 3 phases);
2. Liu sees the impact on egg chick production performance after " yeast culture is on egg chick production performance, Biochemical Indices In Serum and Endocrine Levels impact research " has studied the yeast culture substitute antibiotics such as loyalty, and application in the egg chick produces provides theoretical foundation (Chinese feed .2004 (21) .-17-19) for yeast and culture thereof.
3. Zhang Yuanyuan Zhao Min opens rather. the optimization of compound probiotic fermentation of bacillus culture medium and condition [J]. and Journal of northeast Forestry university, 2012,40 (3): 93-97.
4. model Na old snow peak. the optimization of two kinds of probiotic mixed fermentation whey mediums [J]. Shangluo College's journal, 2011,25 (6): 50-55.
5. Guo Zhi Jie Lijie. the making of lactic acid bacteria preparation and the application [J] on piglet produces thereof. Chinese feed, 2011 (24): 26-29,33.
6. Cui wintersweet appoints the easy minister in ancient times Zhao Jie of emerald green beautiful Zhang Kai to become precious. the bacillus subtilis technique [J] that spreads cultivation. and cultivation and feed, 2011 (12): 41-44.
7. execute the red Hu of Da Lin Sun Mei Chen Qiu and insult the Zhang Weina group that rectifies. the optimization of lactobacillus acidophilus culture medium and High Density Cultivation [J]. food and fermentation science and technology, 2011,47 (2): 60-63.
8. gold stake Peng Jian Hu Xin galaxy of literary talent sound Chinese horse swallow. lactobacillus acidophilus-1.557 condition of culture and medium component optimization research [J]. feed industry, 2010 (2): 34-36.
9. refined Zhu Yu cyanines of Chen Lu Su star Liu Bo flavine Fang Geci. animal feeding probio LPF-2 is to the inhibiting condition of culture optimizations of Escherichia coli [J]. Chinese agronomy circular, 2009 (16): 13-16.
10. open wide quick Wang Wei and open the strong Lin Run of the intelligent fragrant Cui Wei Dongzhanfa of Xiao Linbao peak. the research [J] of Lactobacillus plantarum proliferation of high-density fermentation condition. Xinjiang Agricultural Sciences, 2009,46 (3): 643-650.
The Patents report has CN201110094856.6 disease-resistant growth promoter for aquatic animals and preparation method thereof
CN201110285009.8 antibiotic-free mixed feedstuff and preparation method thereof
CN201210253305.4 pure natural, cheaply Lactobacillus plantarum ST-III cultural method and products thereof and application
CN02136942.9 feeding spore-forming bacillus microbial attitude preparation---glycopeptide element and preparation method thereof
The manufacture method of a CN200310112346.2 probio composite bacteria agent capable
The preparation method of CN201110098451.X probio solid state fermentation complete feed
CN200710078231.4 complex medium that is used for lactobacillus ferment and preparation method thereof
CN200710098254.1 complex medium that is used for bifidobacterium fermentation and preparation method thereof
CN200710171447.5 fermentation material for composite microorganism and preparation method
CN200710151160.6 enzymolysis protein feedstuff and production method thereof that contains profitable probliotics
CN200910034990.X is used for the additive for microbe feedstuff of adjusting energy metabolism impairment of flocks and herds during perinatal period
CN200910072749.6 method for culturing solid nutrient carrier of intestinal probiotics
CN200910004555.2 livestock and poultry edible fungus bran probiotics-containing nutrient solution and preparation method thereof
CN201010110269.7 probiotic active product and preparation method thereof
The preparation method of a CN200810152413.6 probiotic feed additive
CN200810188087.4 probiotic bacteria culture medium and microorganism formulation thereof
CN201010110331.2 hybrid probiotic desiccate and preparation method thereof
CN201010145967.0 is based on the preparation method of the microbial compound preparation of multiple probiotic fermenting cultures
CN200910248915.3 astragalus polysaccharide-oligosaccharide compound probiotic colony culture medium
The method that the solid fermentation of a CN201010010060.3 complex-probiotic group is produced
CN201010213999.X contains the method for manufacturing Monascus of profitable probliotics
The preparation technology of a CN201110041959.6 complex microorganism fermented active feed
The preparation method of CN201110042591.5 biologically active montmorillonite powder and as the application of animal feed additive
CN201010244812.2 natural probiotic bacteria culture medium and probiotics preparation.
Relate generally to manioc waste direct fermentation production feed or feed addictive about manioc waste at the bibliographical information aspect the feed and patent.As: CN201110107304.4 manioc waste biological feed additive for pigs and preparation method thereof, a kind of manioc waste biological feed additive for pigs, the raw material that comprises following percentage by weight: manioc waste 50%-55%, tangerine bar powder 30%-35%, dregs of beans 10%-14%, microorganism fungus kind 1%.CN201210170534.X manioc waste sheep preparation method of Comprehesive biological feed; CN201210210877.4 prepares the method for biological feedstuff etc. take manioc waste as raw material.The present invention utilizes another invention achievement 200910114572.1 manioc wastes of our company to prepare a kind of method of reduced sugar and compound sugar, the manioc waste reduced sugar is made relevant probiotics fermention base-material, there are no relevant report.
The problem that existing disclosed manioc waste probiotics fermention base-material exists is: 1, majority all directly adopts manioc waste, and adopt the manioc waste product of processing less, because crude fibre is too much, probio is not difficult to take full advantage of the nutrient of manioc waste, good 2 for growth, the culture effect of main probio, use grain to be main component more.
Summary of the invention
The present invention mainly solves the problem that exists in the above-mentioned existing probiotics fermention base-material, improves fermentation efficiency, reduces cost, and shortening incubation time, raising viable count, energy savings, minimizing take grain.
Technical scheme of the present invention is:
Probiotics fermention base-material of the present invention is take manioc waste reduced sugar (another invention achievement 200910114572.1 manioc wastes of our company prepare a kind of method of reduced sugar and compound sugar) as main material, composite maize flour, FOS, milk powder, yeast extract, the methods such as employing mixes, fully mixing and get final product.During use, fully mix feeding probio, as: lactobacillus lactis, bifidobacterium bifidum, saccharomyces cerevisiae etc., and add amount of water, after after a while fermentation, product can be used for the expansion of feeding probio to be cultivated, or as the fermented feed base-material.
The preparation method of probiotics fermention base-material of the present invention is:
1) takes by weighing various raw materials by described percentage by weight;
2) above-mentioned various raw materials are put into blending tank, mix and get final product.
The present invention compared with prior art, substantive distinguishing features that it is outstanding and significant progress are:
Take the manioc waste reduced sugar as primary raw material, reduce that grain is taken, reduce product cost; Given full play to the effect of manioc waste, the probio growth efficiency is high, improves the effect of fermentation base-material.
The specific embodiment
Embodiment 1
Manioc waste reduced sugar (containing total reducing sugar 80%) 8000 grams, corn flour 1200 grams, FOS 500 grams, milk powder 200 grams, yeast extract 100 grams in the taper blending tank of packing into, mix namely getting the fermentation base-material in 10 minutes.Using method: add 5000 milliliters of cold water in the base-material, add 15 gram lactobacillus lactis dry powder (viable bacteria content 〉=20,000,000,000 CFU/g), mixing is mixed thoroughly, keeps 30~35 ℃ of temperature, and ferment got final product in 24 hours.Detect through the counting culture dish, viable count surpasses 5 * 10 8/ gram.
Embodiment 2
Manioc waste reduced sugar (containing total reducing sugar 80%) 8000 grams, corn flour 1200 grams, FOS 500 grams, milk powder 200 grams, yeast extract 100 grams in the taper blending tank of packing into, mix namely getting the fermentation base-material in 10 minutes.Using method: add 5000 milliliters of cold water in the base-material, add 20 gram bifidobacterium bifidum dry powder (viable bacteria content 〉=20,000,000,000 CFU/g), mixing is mixed thoroughly, keeps 30~35 ℃ of temperature, and ferment got final product in 24 hours.Detect through the counting culture dish, viable count surpasses 5 * 10 8The CFU/ gram.
Embodiment 3
Manioc waste reduced sugar (containing total reducing sugar 80%) 8000 grams, corn flour 1200 grams, FOS 500 grams, milk powder 200 grams, yeast extract 100 grams in the taper blending tank of packing into, mix namely getting the fermentation base-material in 10 minutes.Using method: add 5000 milliliters of cold water in the base-material, add 10 gram saccharomyces cerevisiae dry powder (viable bacteria content 〉=20,000,000,000 CFU/g), mixing is mixed thoroughly, keeps 30~35 ℃ of temperature, and ferment got final product in 24 hours.Detect through the counting culture dish, viable count surpasses 5 * 10 8The CFU/ gram.

Claims (1)

1. feeding probiotics fermention base-material, it is characterized in that: described fermentation base-material is by weight percentage: manioc waste reduced sugar 80%, corn flour 12%, FOS 5%, milk powder 2%, yeast extract 1%.
CN 201210589745 2012-12-31 2012-12-31 Feed probiotic fermented base material Pending CN102987062A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103355498A (en) * 2013-07-26 2013-10-23 河南碧云天饲料有限公司 Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103355498A (en) * 2013-07-26 2013-10-23 河南碧云天饲料有限公司 Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs
CN103355498B (en) * 2013-07-26 2014-10-22 河南碧云天饲料有限公司 Biological feed composite, compound premix and milk replacer without antibiotic or plasma protein for suckling pigs

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Application publication date: 20130327