CN102986742A - application of astragalus mongholicus water extract to biopesticides - Google Patents
application of astragalus mongholicus water extract to biopesticides Download PDFInfo
- Publication number
- CN102986742A CN102986742A CN2012105515715A CN201210551571A CN102986742A CN 102986742 A CN102986742 A CN 102986742A CN 2012105515715 A CN2012105515715 A CN 2012105515715A CN 201210551571 A CN201210551571 A CN 201210551571A CN 102986742 A CN102986742 A CN 102986742A
- Authority
- CN
- China
- Prior art keywords
- water extract
- radix astragali
- astragali water
- mother liquor
- purposes according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of an astragalus mongholicus water extract to biopesticides and particularly relates to application of the astragalus mongholicus water extract to weeding, and resistance to wheat glume blight and early blight of plants. The astragalus mongholicus water extract provided by the invention is capable of effectively weeding and inhibiting pathogenic bacteria of the wheat glume blight and the early blight of plants, and the selection range of the biopesticides is broadened.
Description
Technical field
The present invention relates to a kind of new purposes of Radix Astragali water extract, be specifically related to the application of Radix Astragali water extract aspect agricultural chemicals.
Background technology
The weeds that the whole world extensively distributes have 30000 kinds, and annual about 1800 kinds are caused in various degree harm to crop, and the annual crop production reduction that causes because of weeds harm is up to 9.7%.Although adopt chemical herbicide effectively to control many weeds, a large amount of uses of chemical agent have also caused a lot of bad problems, such as the degeneration of the appearance of herbicide-resistant weeds plant, soil pollution, water quality and to the harm of people, animal etc.
The former bacterium of wheat glume blight (Septoria nodorum) is one of the main pathogenic fungi that causes wheat glume blight, mainly cause harm wheat prematurity fringe section and stem stalk, also cause harm blade and leaf sheath.After fringe section catches an illness, first on the top or the top small ear occur, begin to be the dark brown spot on the clever shell, after become withered white and expand to whole clever shell, cover with mycelia and pore (pycnidium) on it; Be the brown scab after stipes is caught an illness, can invade conduit and with its obstruction, the distortion of envoy section, distortion, top stem stalk fractures and dead; After blade is caught an illness, just be the filbert stigma of spindle shape, after be expanded into the large spot of irregular shape, there is yellowish haloing at the edge, central canescence, close living pore on it, the killed distortion of sword-like leave is withered.Flavescence after the leaf sheath morbidity makes blade early withered.The harm of wheat glume blight is larger, has become the important disease of a class on China's Wheat Production, and rainy time and humid area occur especially serious.
Early blight pathogen (Alternaria solani) is the pathogen that causes plant of Solanaceae early blight, the vegetables such as tomato, potato, capsicum, eggplant of mainly causing harm.How in the morbidity in seedling stage, blade tip or terminal bud produce the water stain shape scab of crineous, cause rottenly, and feel is smooth, after rotting in seedling top, form without the top seedling, in addition mashed to the native face of bed, cause seedling stage just dead, endanger very large.
In order to prevent and treat disease, people increase resistance by breeding resistant variety on the one hand, carry out on the one hand chemical control.The former has certain limitation, and the latter can bring environmental pollution.Therefore the novel agrochemical of efficient, the low toxicity of exploitation, low-residual especially biogenic pesticide more and more come into one's own.
Milkvetch Root derives from the astragalus mongolicus into leguminous herbaceous plant, the root of Astragalus membranacus, claim again cotton stilbene, continuous stilbene, the continuous Radix Astragali (" Bencao Tujing "), the cotton Radix Astragali, yellow alpine yarrow, yellow over sixty years of age, arrow stilbene (Liu Shilian " medical science is integrated "), the arrow Radix Astragali, astragalus mongholicus Bge, prince's descendants' (" property of medicine opinion "), wear stilbene, wear crushed grain (" herbal classic "), wear mulberry, only mulberry, another name for Sichuan Province fat, hundred (" not Lu "), hundred absorbent cotton, hundred medicines continuous (Hou Ningji " medicine spectrum "), soil quick-fried root in mountain (" Xinjiang medicinal material "), the only son and heir (" Gansu Chinese medicine handbook), take out greatly, two people lift (" Liaoning economic plants will "), stilbene is arranged greatly, cover stilbene, unit's stilbene, red blue stilbene, RADIX ASTRAGALI, the casting skin stilbene, Astragalus membranacus, astragalus membranaceus, Inner Mongolia Astragalis, rush positive stilbene, Wuchuan stilbene.The Radix Astragali has the effects such as invigorating qi for strengthening superficies, diuresis holder poison, apocenosis, expelling pus and promoting granulation, be used for the treatment of that the deficiency of vital energy is weak, anorexia and loose stool, the sinking of qi of middle-jiao, for a long time rush down prolapse of the anus, the uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, ulcer difficultly burst, burst for a long time do not hold back, the deficiency of blood is sallow, interior heat is quenched one's thirst etc., modern study shows that the Radix Astragali has to be strengthened special, non-specific immune function, cardiac stimulant, antiviral property myocarditis, antiulcer, increase RBC number, protects the liver, antifatigue, antidotal effect, regulate the effect such as blood pressure.But its application aspect agricultural chemicals has no report.
Summary of the invention
In order to develop new biogenic pesticide, the invention provides the purposes of Radix Astragali water extract aspect agricultural chemicals, relate generally to cut weeds (Brassica rapa L.Chinensis Group), anti-wheat glume blight (Septoria nodorum) and anti-plant of Solanaceae early blight (Alternaria solani).
Radix Astragali water extract is prepared into mother liquor with the DMSO dissolving first when using.
Being applied in when extremely cutting weeds, is 60 μ g/cm2 with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to Radix Astragali ethanol extract.
When being applied in control plant of Solanaceae early blight and wheat glume blight, be 20～200ppm (mg/L) with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to Radix Astragali ethanol extract.
The present invention has the following advantages compared to existing technology: utilize Radix Astragali water extract as weeding, antibacterial agent, effectively weeding, Suppressing phytopathogens; Radix Astragali water extract wide material sources; Radix Astragali water extract is nontoxic to people and animals, and easily degraded can not produce and produce " three R " in the chemical pesticide use procedure, to the less pollution of environment.
Embodiment
Below in conjunction with specific embodiment Radix Astragali water extract of the present invention is elaborated in the application aspect the agricultural chemicals.
Test sample: Milkvetch Root is purchased from Nanjing medicine company limited company.
Experimental instrument: high speed Universalpulverizer; Vertical automatic pressure steam sterilizer; Shaking table; The ultrasonic oscillation instrument; Rotary Evaporators; Thermostat water bath; Electronic balance; The intelligence climatic cabinate; Vavuum pump.
Extracting method: filter Milkvetch Root boils 2 hours in the water of 8 times of amounts (W/V) after, filter residue boils with 8 times of water gagings and filters after 2 hours, reduced pressure concentration after filtrate merges, and 60 ℃ of lower dryings get brown medicinal extract.
Mother liquor configuration: get Radix Astragali water extract (brown medicinal extract) with the DMSO dissolving, be prepared into the mother liquor that concentration is 10mg/mL.
The configuration of test solution: during use mother liquor is diluted to debita spissitudo with 0.025%Tween 20.
Embodiment one
Careless for examination: rape (Brassica rapa L.Chinensis Group), Bermuda grass (Cynodon dactylon L.), duckweed (Lemna minor) are provided by the academy of agricultural sciences, Hubei Province.
Test method: screening technique is agar medium surface-coated method when adopting a variety of Chinese cabbage and Bermuda grass to be target.Adopt 96 porocyte culture plates, every hole adds entry agar 300 μ L and about 10 seeds, then adds dilute liquid medicine 20 μ L, and the primary dcreening operation liquor strength is 1.0mg/mL, 0.3mg/mL, 0.1mg/mL.After one week, check seed germination and plant strain growth situation.Sieving again concentration adjusts according to the primary dcreening operation result.
Adopt water culture during take duckweed as target.Adopt 24 porocyte culture plates, every hole adds duckweed and cultivates nutrient solution 2mL, accesses 3 duckweed blades, adds dilute liquid medicine 20 μ L again.Liquor strength is 1.0mg/mL during primary dcreening operation, checks the growing state of duckweed after the week.Sieving again concentration adjusts according to the primary dcreening operation result.
When sieving again, be 60ug/cm to the concentration of rape and Bermuda grass
2, be 10ppm to the concentration of duckweed.
Result of the test: Radix Astragali water extract is 60 μ g/em in concentration
2In time, suppress fully to rape, and duckweed and Bermuda grass are not had inhibitory action, has certain selectivity.
Embodiment two
For trying bacterium: Pythium ultimum bacterium (Pythium ultimum), botrytis cinerea (Botrytis cinerea), the former bacterium of wheat glume blight (Septoria nodorum), early blight pathogen (Alternaria Solani), gibberella saubinetii cause of disease bacterium (Fusariumculmorum), the former bacterium of damping off (Rhizoctonia solani) are provided by the academy of agricultural sciences, Hubei Province.
Test method: semisolid culturemedium method.Adopt 96 porocyte culture plates, every hole adds the mycelium of 90 μ L medium and fungi to be measured or the mixture of spore, and it is 10 μ L that every hole adds amount of liquid medicine.Primary dcreening operation concentration is 20ppm, sieves again concentration and adjusts according to the primary dcreening operation result.
Result of the test: Radix Astragali water extract has stronger inhibitory action to the former bacterium of wheat glume blight and plant of Solanaceae early blight pathogen when concentration is 20ppm, inhibiting rate is 77%.
Claims (7)
1. the application of Radix Astragali water extract aspect agricultural chemicals.
2. the described Radix Astragali water extract of claim 1 is: Milkvetch Root usefulness water boil filtered after 2 hours, the brown medicinal extract of filtrate concentrate drying gained.
3. purposes according to claim 1 is characterized in that: described Radix Astragali water extract is used for cutting weeds.
4. purposes according to claim 3 is characterized in that: described Radix Astragali water extract adopts first DMSO dissolving preparation mother liquor when using, getting described mother liquor before the use is 60 μ g/cm with the application concentration that 0.025% polysorbas20 is diluted to Radix Astragali water extract
2
5. purposes according to claim 1, it is characterized in that: described Radix Astragali water extract can suppress the former bacterium of wheat glume blight, is used for control wheat glume blight.
6. purposes according to claim 1, it is characterized in that: described Radix Astragali water extract can suppress plant early blight pathogen, is used for control plant of Solanaceae early blight.
7. described purposes according to claim 5-6 is characterized in that: described Radix Astragali water extract adopts first DMSO dissolving preparation mother liquor when using, and gets described mother liquor is diluted to Radix Astragali water extract with 0.025% polysorbas20 concentration before using and is 20ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105515715A CN102986742A (en) | 2012-12-18 | 2012-12-18 | application of astragalus mongholicus water extract to biopesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105515715A CN102986742A (en) | 2012-12-18 | 2012-12-18 | application of astragalus mongholicus water extract to biopesticides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102986742A true CN102986742A (en) | 2013-03-27 |
Family
ID=47916229
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105515715A Pending CN102986742A (en) | 2012-12-18 | 2012-12-18 | application of astragalus mongholicus water extract to biopesticides |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102986742A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957184A (en) * | 2015-08-06 | 2015-10-07 | 杨新兰 | Bactericide containing epoxiconazole and radix astragali |
| CN104957185A (en) * | 2015-08-06 | 2015-10-07 | 杨新兰 | Bactericide containing tebuconazole and radix astragali |
| CN104982463A (en) * | 2015-08-06 | 2015-10-21 | 杨新兰 | Bactericide containing coumoxystrobin and astragalus membranaceus |
| CN105053040A (en) * | 2015-08-06 | 2015-11-18 | 杨新兰 | Bactericide containing propiconazole and radix astragali |
| CN105053039A (en) * | 2015-08-06 | 2015-11-18 | 杨新兰 | Bactericide containing hexaconazole and radix astragali |
| CN105145654A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing azoxystrobin and radix astragali |
| CN105145655A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing carbendazim and radix astragali |
| CN105145653A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing tetraconazole and radix astragali |
| CN105145652A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing thifluzamide and radix astragali |
| CN106889124A (en) * | 2017-03-09 | 2017-06-27 | 海门市利美农副产品有限公司 | A kind of Chinese medicine herbicide |
| CN109430298A (en) * | 2018-12-29 | 2019-03-08 | 南京高正农用化工有限公司 | A kind of big granula of paddy field weed-killer and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664432A (en) * | 2008-09-02 | 2010-03-10 | 天津生机集团股份有限公司 | Method for preparing microbial fermentation preparations of astragalus roots |
| CN102805111A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Plant medicament for preventing and treating mustard aphids |
-
2012
- 2012-12-18 CN CN2012105515715A patent/CN102986742A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664432A (en) * | 2008-09-02 | 2010-03-10 | 天津生机集团股份有限公司 | Method for preparing microbial fermentation preparations of astragalus roots |
| CN102805111A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Plant medicament for preventing and treating mustard aphids |
Non-Patent Citations (1)
| Title |
|---|
| 于志同等: "34种植物丙酮提取物抑菌活性的初步筛选", 《西北农林科技大学学报（自然科学版）》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957184A (en) * | 2015-08-06 | 2015-10-07 | 杨新兰 | Bactericide containing epoxiconazole and radix astragali |
| CN104957185A (en) * | 2015-08-06 | 2015-10-07 | 杨新兰 | Bactericide containing tebuconazole and radix astragali |
| CN104982463A (en) * | 2015-08-06 | 2015-10-21 | 杨新兰 | Bactericide containing coumoxystrobin and astragalus membranaceus |
| CN105053040A (en) * | 2015-08-06 | 2015-11-18 | 杨新兰 | Bactericide containing propiconazole and radix astragali |
| CN105053039A (en) * | 2015-08-06 | 2015-11-18 | 杨新兰 | Bactericide containing hexaconazole and radix astragali |
| CN105145654A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing azoxystrobin and radix astragali |
| CN105145655A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing carbendazim and radix astragali |
| CN105145653A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing tetraconazole and radix astragali |
| CN105145652A (en) * | 2015-08-06 | 2015-12-16 | 杨新兰 | Bactericide containing thifluzamide and radix astragali |
| CN106889124A (en) * | 2017-03-09 | 2017-06-27 | 海门市利美农副产品有限公司 | A kind of Chinese medicine herbicide |
| CN109430298A (en) * | 2018-12-29 | 2019-03-08 | 南京高正农用化工有限公司 | A kind of big granula of paddy field weed-killer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102986742A (en) | application of astragalus mongholicus water extract to biopesticides | |
| CN101851356B (en) | Shitake mushroom extract and application thereof | |
| CN100463604C (en) | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice | |
| CN102217661B (en) | Botanical bactericide and preparation method and application thereof | |
| CN102177921B (en) | Compound biological control agent as well as preparation method and application thereof | |
| JP2008528523A (en) | A method of using metabolites of Alternaria alternata in purple stem bud flowers for biological weeding | |
| Oriabi | Moringa oleifera in vitro culture and its application as anti-diabetic in alloxan induced diabetic albino mice | |
| CN107114416A (en) | Prevent and treat the botanical fungicide of rice blast | |
| CN104488977B (en) | Bark of Pterocarya Stenoptera and berry extract and its new application | |
| CN105407725B (en) | The composition and method of selectivity control invasive species | |
| KR101433209B1 (en) | Hydroponic Culture Media for Ginseng and Preparation Method Thereof | |
| Dadkhah | Allelopathic effect of sugar beet (Beta vulgaris) and eucalyptus (Eucalyptus camaldulensis) on seed germination and growth of Portulaca oleracea | |
| CN102283235B (en) | Postemergence formulated herbicide used for cotton field | |
| CN106576769A (en) | Watermelon seedling cultivation method capable of resisting continuous cropping obstacles | |
| Rabothata | Interaction of vesicular arbuscular mycorrhiza, nematode and phytonematicides on growth and nutritional content of Cleome gynandra | |
| CN103242085A (en) | Organic plant foliar fertilizer and preparation and spraying methods of organic plant foliar fertilizer | |
| CN109938055A (en) | It is a kind of for preventing and treating the composition pesticide of rice sheath blight disease | |
| CN106305262A (en) | Planting method of Zijuan tea young seedlings | |
| CN1315387C (en) | Use of gingko endogenous fungus metabolite in preventing and treating plant fungus disease | |
| CN102986738A (en) | Application of poria cocos ethanol extract to biopesticides | |
| CN104838863B (en) | A kind of method for cultivating specific health-care efficacy crops | |
| CN104920480B (en) | A kind of plant insecticide and preparation method thereof | |
| Khurshid et al. | Growth responses of Parthenium hysterophorus L. growing under salt stress. | |
| RAZA | Role of Jasmonic Acid in Mitigating Mancozeb Induced Toxicity in Vicia faba | |
| CN108849899A (en) | A kind of compound bio medicament for preventing and treating peanut leaf diseases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130327 |