CN102986738A - Application of poria cocos ethanol extract to biopesticides - Google Patents
Application of poria cocos ethanol extract to biopesticides Download PDFInfo
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- CN102986738A CN102986738A CN2012105499322A CN201210549932A CN102986738A CN 102986738 A CN102986738 A CN 102986738A CN 2012105499322 A CN2012105499322 A CN 2012105499322A CN 201210549932 A CN201210549932 A CN 201210549932A CN 102986738 A CN102986738 A CN 102986738A
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- poria cocos
- ethanol extract
- cocos ethanol
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- blight
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Abstract
The invention discloses application of a poria cocos ethanol extract to biopesticides and particularly relates to application of the poria cocos ethanol extract to killing with poison of cotton bollworm and resistance to wheat glume blight and early blight of plants. The poria cocos ethanol extract provided by the invention is capable of effectively killing the cotton bollworm with poison and resisting the wheat glume blight and the early blight of plants, and the selection range of the biopesticides is broadened.
Description
Technical field
The present invention relates to a kind of new purposes of Poria cocos ethanol extract, be specifically related to the application of Poria cocos ethanol extract aspect agricultural chemicals.
Background technology
Cotton bollworm (Heliothis armigera) is the Lepidoptera noctuid, and host range is wide, and fertility is strong, is Asia, Oceania, Africa and European various places one of the primary pest of cotton and other crops of causing harm, and is global crop pests.Since the nineties, cotton bollworm obviously increases in main cotton producing region outbreak year frequencies such as China the Yellow River, the Changjiang river, Liaohe River Basin and Xinjiang, causes cotton underproduction rate up to 35～50%, part field even total crop failure that some is regional from eighties of last century.
Wheat glume blight bacterium (Septoria nodorum) is one of the main pathogenic fungi that causes wheat glume blight, mainly cause harm wheat prematurity fringe section and stem stalk, also cause harm blade and leaf sheath.After fringe section catches an illness, first on the top or the top small ear occur, begin to be the dark brown spot on the clever shell, after become withered white and expand to whole clever shell, cover with mycelia and pore (pycnidium) on it; Be the brown scab after stipes is caught an illness, can invade conduit and with its obstruction, the distortion of envoy section, distortion, top stem stalk fractures and dead; After blade is caught an illness, just be the filbert stigma of spindle shape, after be expanded into the large spot of irregular shape, there is yellowish haloing at the edge, central canescence, close living pore on it, the killed distortion of sword-like leave is withered.Flavescence after the leaf sheath morbidity makes blade early withered.The harm of wheat glume blight is larger, has become the important disease of a class on China's Wheat Production, and rainy time and humid area occur especially serious.
Early epidemic germ (Alternaria solani) is the pathogen that causes plant of Solanaceae early blight, the vegetables such as tomato, potato, capsicum, eggplant of mainly causing harm.How in the morbidity in seedling stage, blade tip or terminal bud produce the water stain shape scab of crineous, cause rottenly, and feel is smooth, after rotting in seedling top, form without the top seedling, in addition mashed to the native face of bed, cause seedling stage just dead, endanger very large.
In order to prevent and treat disease, people increase resistance by breeding resistant variety on the one hand, carry out on the one hand chemical control.The former has certain limitation, and the latter can bring environmental pollution.Therefore the novel agrochemical of efficient, the low toxicity of exploitation, low-residual especially biogenic pesticide more and more come into one's own.
Poria cocos is the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf, another name Fu Tu, the Fu spirit, Fu Ling, Fu Ling, volt Tu, the pine fat, lie prostrate tire deep red morning, Yun Ling, the Fu rabbit, the pine potato, the pine potato, Song Ling, nature and flavor are sweet, light, flat, thoughts of returning home warp, lung channel, the spleen channel, kidney channel, cure mainly difficult urination, the oedema turgor, phlegm and retained fluid is coughed contrary, vomiting, insufficiency of the spleen food is few, have loose bowels, palpitation and restlessness, insomnia forgetfulness, the seminal emission gonorrhoea, modern study shows that it has diuresis, suppress staphylococcus aureus, Escherichia coli, proteus, antitumor, caused by cyclophosphamide big white mouse leucocyte is reduced to accelerate, improve the effects such as immunity.But its application aspect agricultural chemicals is not arranged.
Summary of the invention
In order to develop new biogenic pesticide, the invention provides the purposes of Poria cocos ethanol extract aspect agricultural chemicals, relate generally to poisoning cotton bollworm (Heliothis armigera), anti-wheat glume blight (Septoria nodorum) and anti-plant early blight (Alternaria solani).
The Poria cocos ethanol extract is prepared into mother liquor with the DMSO dissolving first when using.
When being applied in the control cotton bollworm, be 60 μ g/cm with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to the Poria cocos ethanol extract
2
When being applied in control plant of Solanaceae early blight and wheat glume blight, be 20～200ppm (mg/L) with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to the Poria cocos ethanol extract.
The present invention has the following advantages compared to existing technology: utilize the Poria cocos ethanol extract as desinsection, antibacterial agent, effectively desinsection, Suppressing phytopathogens; Poria cocos ethanol extract wide material sources; The Poria cocos ethanol extract is nontoxic to people and animals, and easily degraded can not produce and produce " three R " in the chemical pesticide use procedure, to the less pollution of environment.
Embodiment
Below in conjunction with specific embodiment Poria cocos ethanol extract of the present invention is elaborated in the application aspect the agricultural chemicals.
Test sample: the Poria cocos medicinal material is purchased from Nanjing medicine company limited company.
Experimental instrument: high speed Universalpulverizer; Vertical automatic pressure steam sterilizer; Shaking table; The ultrasonic oscillation instrument; Rotary Evaporators; Thermostat water bath; Electronic balance; The intelligence climatic cabinate; Vavuum pump.
Extracting method: the Poria cocos medicinal material filters after 2 hours with 8 times of amount 70% ethanol (W/V) refluxing extraction, and filter residue filters after 2 hours with the extraction of 8 times of amount 70% alcohol refluxs again, and filtrate merges, reduced pressure concentration, and 60 ℃ are lower dry, get brown medicinal extract.
Mother liquor configuration: get Poria cocos ethanol extract (brown medicinal extract) with the DMSO dissolving, be prepared into the mother liquor that concentration is 10mg/mL.
The configuration of test solution: during use mother liquor is diluted to debita spissitudo with 0.025%Tween 20.
Embodiment one
For trying insect: cotton bollworm (Heliothis armigera), diamond-back moth (Plutella xylostella) and bean aphid (Aphis craccivora) are provided by the academy of agricultural sciences, Hubei Province.
Test method: during take diamond-back moth and cotton bollworm as the examination worm, ovum adopts the feed formula through academy of agricultural sciences, improved Beijing beam leather plum, the indoors artificial raising with 10% formalin sterilization 15min.The raising condition: 25 ℃ of temperature, relative moisture are 75%-85%.Sample Dilution is coated the artificial feed surface to finite concentration.Adopt 96 porocyte culture plates, every hole adds artificial feed 300 μ L and dilute liquid medicine 20 μ L, checking experiment result after the week.Liquor strength is 1.0mg/mL during primary dcreening operation, sieves again concentration and adjusts according to the primary dcreening operation result.
Adopt infusion method during take bean aphid as the examination worm.Primary dcreening operation concentration is 1.0mg/mL, directly has the bean seedlings of bean aphid to be immersed in the liquid with long, check result after the week.Sieving again concentration adjusts according to the primary dcreening operation result.
Result of the test: the Poria cocos ethanol extract is 60 μ g/cm in concentration
2The time cotton bollworm had 100% toxic action, to diamond-back moth and bean aphid without the poisoning effect.
Embodiment two
For trying bacterium: Pythium ultimum bacterium (Pythium ultimum), botrytis cinerea (Botrytis cinerea), the former bacterium of wheat glume blight (Septoria nodorum), early blight pathogen (Alternaria Solani), gibberella saubinetii cause of disease bacterium (Fusarium culmorum), damping off pathogen (Rhizoctonia solani) are provided by the academy of agricultural sciences, Hubei Province.
Test method: semisolid culturemedium method.Adopt 96 porocyte culture plates, every hole adds the mycelium of 90 μ L medium and fungi to be measured or the mixture of spore, and it is 10 μ L that every hole adds amount of liquid medicine.Primary dcreening operation concentration is 20ppm, sieves again concentration and adjusts according to the primary dcreening operation result.
Result of the test: the Poria cocos ethanol extract has stronger inhibitory action to the former bacterium of wheat glume blight and early blight pathogen when concentration is 20ppm, and inhibiting rate is 77%.
Claims (7)
1. the purposes of Poria cocos ethanol extract aspect agricultural chemicals.
2. Poria cocos ethanol extract claimed in claim 1 is: the Poria cocos medicinal material filters after extracting with 60%～100% alcohol reflux, the brown medicinal extract of concentrated gained.
3. purposes according to claim 1 is characterized in that: described Poria cocos ethanol extract is used for the poisoning cotton bollworm.
4. purposes according to claim 3, it is characterized in that: described Poria cocos ethanol extract is when using, adopt first DMSO dissolving preparation mother liquor, getting described mother liquor before the use is 60 μ g/cm with 0.025% polysorbas20 solution dilution to the application concentration of Poria cocos ethanol extract
2
5. purposes according to claim 1, it is characterized in that: described Poria cocos ethanol extract can suppress the former bacterium of wheat glume blight, is used for control wheat glume blight.
6. purposes according to claim 1, it is characterized in that: described Poria cocos ethanol extract can suppress the early blight pathogen, is used for control plant of Solanaceae early blight.
7. described purposes according to claim 5-6, it is characterized in that: described Poria cocos ethanol extract is when using, adopt first DMSO dissolving preparation mother liquor, get described mother liquor before using and be 20ppm with the application concentration that 0.025% polysorbas20 is diluted to the Poria cocos ethanol extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105499322A CN102986738A (en) | 2012-12-18 | 2012-12-18 | Application of poria cocos ethanol extract to biopesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105499322A CN102986738A (en) | 2012-12-18 | 2012-12-18 | Application of poria cocos ethanol extract to biopesticides |
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| Publication Number | Publication Date |
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| CN102986738A true CN102986738A (en) | 2013-03-27 |
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| CN2012105499322A Pending CN102986738A (en) | 2012-12-18 | 2012-12-18 | Application of poria cocos ethanol extract to biopesticides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108606012A (en) * | 2018-05-03 | 2018-10-02 | 蒋德云 | A kind of pesticide and preparation method thereof of the prevention viroses of plant |
| KR20230065692A (en) * | 2021-11-05 | 2023-05-12 | 충청북도 | Composition for controlling plant diseases comprising basidiomycetes mycelium or culture broth |
Citations (3)
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| US3249493A (en) * | 1963-01-29 | 1966-05-03 | Koppers Co Inc | Repelling termites with the fungus poria monticola |
| CN101427694A (en) * | 2007-11-08 | 2009-05-13 | 冷雪飞 | Natural green biological organic agricultural chemical composition and uses thereof |
| CN102133242A (en) * | 2011-03-07 | 2011-07-27 | 北京中医药大学 | Tuckahoe extract and preparation method thereof |
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2012
- 2012-12-18 CN CN2012105499322A patent/CN102986738A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3249493A (en) * | 1963-01-29 | 1966-05-03 | Koppers Co Inc | Repelling termites with the fungus poria monticola |
| CN101427694A (en) * | 2007-11-08 | 2009-05-13 | 冷雪飞 | Natural green biological organic agricultural chemical composition and uses thereof |
| CN102133242A (en) * | 2011-03-07 | 2011-07-27 | 北京中医药大学 | Tuckahoe extract and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王树桐等: "126种中草药提取物对2种植物病原真菌的抑制作用", 《河南农业科学》, no. 10, 15 October 2006 (2006-10-15), pages 62 - 65 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108606012A (en) * | 2018-05-03 | 2018-10-02 | 蒋德云 | A kind of pesticide and preparation method thereof of the prevention viroses of plant |
| KR20230065692A (en) * | 2021-11-05 | 2023-05-12 | 충청북도 | Composition for controlling plant diseases comprising basidiomycetes mycelium or culture broth |
| KR102629547B1 (en) * | 2021-11-05 | 2024-01-29 | 충청북도 | Composition for controlling plant diseases comprising basidiomycetes mycelium or culture broth |
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Application publication date: 20130327 |