CN102960371A - Application of radix trichosanthis water extract to biological pesticides - Google Patents
Application of radix trichosanthis water extract to biological pesticides Download PDFInfo
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- CN102960371A CN102960371A CN2012105513692A CN201210551369A CN102960371A CN 102960371 A CN102960371 A CN 102960371A CN 2012105513692 A CN2012105513692 A CN 2012105513692A CN 201210551369 A CN201210551369 A CN 201210551369A CN 102960371 A CN102960371 A CN 102960371A
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- water extract
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- chinese trichosanthes
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- blight
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Abstract
The invention discloses an application of a radix trichosanthis water extract to pesticides, and particularly relates to an application of the trichosanthis water extract to the resistance of wheat septoria nodorum blotch and plant early blight. The radix trichosanthis water extract is capable of completely suppressing the wheat septoria nodorum blotch pathogenic bacteria and the plant early blight pathogenic bacteria, so that the selection range of the biological pesticides is enlarged.
Description
Technical field
The present invention relates to a kind of new purposes of root of Chinese trichosanthes water extract, be specifically related to the application of root of Chinese trichosanthes water extract aspect agricultural chemicals.
Background technology
The former bacterium of wheat glume blight (Septoria nodorum) is one of the main pathogenic fungi that causes wheat glume blight, mainly cause harm wheat prematurity fringe section and stem stalk, also cause harm blade and leaf sheath.After fringe section catches an illness, first on the top or the top small ear occur, begin to be the dark brown spot on the clever shell, after become withered white and expand to whole clever shell, cover with mycelia and pore (pycnidium) on it; Be the brown scab after stipes is caught an illness, can invade conduit and with its obstruction, the distortion of envoy section, distortion, top stem stalk fractures and dead; After blade is caught an illness, just be the filbert stigma of spindle shape, after be expanded into the large spot of irregular shape, there is yellowish haloing at the edge, central canescence, close living pore on it, the killed distortion of sword-like leave is withered.Flavescence after the leaf sheath morbidity makes blade early withered.The harm of wheat glume blight is larger, has become the important disease of a class on China's Wheat Production, and rainy time and humid area occur especially serious.
Early blight pathogen (Alternaria solani) is the pathogen that causes plant of Solanaceae early blight, the vegetables such as tomato, potato, capsicum, eggplant of mainly causing harm.How in the morbidity in seedling stage, blade tip or terminal bud produce the water stain shape scab of crineous, cause rottenly, and feel is smooth, after rotting in seedling top, form without the top seedling, in addition mashed to the native face of bed, cause seedling stage just dead, endanger very large.
In order to prevent and treat disease, people increase resistance by breeding resistant variety on the one hand, carry out on the one hand chemical control.The former has certain limitation, and the latter can bring environmental pollution.Therefore the novel agrochemical of efficient, the low toxicity of exploitation, low-residual especially biogenic pesticide more and more come into one's own.
Root of Chinese trichosanthes, another name radices trichosanthis, pollen, building root, baiyao, timely snow, day melon powder, dung melon root, Chinese juniper beach wormwood powder, the beach wormwood powder is the dry root of cucurbitaceous plant snakegourd Trichosanthes kirilowii Maxim. or trichosanthes rosthornii Harms Trichosanthes rosthornii Herms.It is sweet to distinguish the flavor of; Little hardship; Cold nature.Return lung channel; The stomach warp.It is thirsty to cure mainly pyreticosis; Quench one's thirst and drink more; Lung-heat type cough; Sore swollen toxin.Medicinal material whole nation most areas has product.The ground such as main product Henan, Guangxi, Shandong, Jiangsu, Guizhou, Anhui.Modern pharmacological research shows that it has anticancer, anti-early pregnancy, antibiotic, antiviral, enhancing mouse immune effect, but its application aspect agricultural chemicals has no report.
Summary of the invention
In order to develop new biogenic pesticide, the invention provides the purposes of root of Chinese trichosanthes water extract aspect agricultural chemicals, relate generally to anti-wheat glume blight (Septoria nodorum) and anti-plant of Solanaceae early blight (Alternaria solani).
The root of Chinese trichosanthes water extract is prepared into mother liquor with the DMSO dissolving first when using.
When being applied in control plant of Solanaceae early blight and wheat glume blight, be 20～200ppm (mg/L) with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to the root of Chinese trichosanthes ethanol extract.
The present invention has the following advantages compared to existing technology: utilize the root of Chinese trichosanthes water extract as antibacterial agent, fully Suppressing phytopathogens; Root of Chinese trichosanthes water extract wide material sources; The root of Chinese trichosanthes water extract is nontoxic to people and animals, and easily degraded can not produce and produce " three R " in the chemical pesticide use procedure, to the less pollution of environment.
Embodiment
Below in conjunction with specific embodiment root of Chinese trichosanthes water extract of the present invention is elaborated in the application aspect the agricultural chemicals.
Test sample: the root of Chinese trichosanthes medicinal material is purchased from Nanjing medicine company limited company.
Experimental instrument: high speed Universalpulverizer; Vertical automatic pressure steam sterilizer; Shaking table; The ultrasonic oscillation instrument; Rotary Evaporators; Thermostat water bath; Electronic balance; The intelligence climatic cabinate; Vavuum pump.
Extracting method: filter the root of Chinese trichosanthes medicinal material boils 2 hours in the water of 8 times of amounts (W/V) after, filter residue boils with 8 times of water gagings and filters after 2 hours, reduced pressure concentration after filtrate merges, and 60 ℃ of lower dryings get brown medicinal extract.
Mother liquor configuration: get root of Chinese trichosanthes water extract (brown medicinal extract) with the DMSO dissolving, be prepared into the mother liquor that concentration is 10mg/mL.
The configuration of test solution: during use mother liquor is diluted to debita spissitudo with 0.025%Tween 20.
Embodiment
For trying bacterium: Pythium ultimum bacterium (Pythium ultimum), botrytis cinerea (Botrytis cinerea), the former bacterium of wheat glume blight (Septoria nodorum), early blight pathogen (Alternaria Solani), gibberella saubinetii cause of disease bacterium (Fusariumculmorum), the former bacterium of damping off (Rhizoctonia solani) are provided by the academy of agricultural sciences, Hubei Province.
Test method: semisolid culturemedium method.Adopt 96 porocyte culture plates, every hole adds the mycelium of 90 μ L medium and fungi to be measured or the mixture of spore, and it is 10 μ L that every hole adds amount of liquid medicine.Primary dcreening operation concentration is 20ppm, sieves again concentration and adjusts according to the primary dcreening operation result.
Result of the test: the root of Chinese trichosanthes water extract has very strong inhibitory action to the former bacterium of wheat glume blight and plant of Solanaceae early blight pathogen when concentration is 20ppm, inhibiting rate is 100%.
Claims (5)
1. the application of root of Chinese trichosanthes water extract aspect agricultural chemicals.
2. the described root of Chinese trichosanthes water extract of claim 1 is: root of Chinese trichosanthes medicinal material usefulness water boil filtered after 2 hours, the brown medicinal extract of filtrate concentrate drying gained.
3. purposes according to claim 1, it is characterized in that: described root of Chinese trichosanthes water extract can suppress the former bacterium of wheat glume blight, is used for control wheat glume blight.
4. purposes according to claim 1, it is characterized in that: described root of Chinese trichosanthes water extract can suppress plant early blight pathogen, is used for control plant of Solanaceae early blight.
5. described purposes according to claim 3-4 is characterized in that: described root of Chinese trichosanthes water extract adopts first DMSO dissolving preparation mother liquor when using, and gets described mother liquor is diluted to the root of Chinese trichosanthes water extract with 0.025% polysorbas20 concentration before using and is 20ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105513692A CN102960371A (en) | 2012-12-18 | 2012-12-18 | Application of radix trichosanthis water extract to biological pesticides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105513692A CN102960371A (en) | 2012-12-18 | 2012-12-18 | Application of radix trichosanthis water extract to biological pesticides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102960371A true CN102960371A (en) | 2013-03-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105513692A Pending CN102960371A (en) | 2012-12-18 | 2012-12-18 | Application of radix trichosanthis water extract to biological pesticides |
Country Status (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651615A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Biological insecticide containing lycopene and preparation method of biological insecticide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1109708A (en) * | 1994-12-28 | 1995-10-11 | 黄可荣 | Plant pesticide composition and preparing process thereof |
| WO2007139382A2 (en) * | 2006-05-29 | 2007-12-06 | Praktijkonderzoek Plant & Omgeving B.V. | Method for controlling a fungal foliar pathogens with an extract from yucca |
| CN101449804A (en) * | 2008-12-23 | 2009-06-10 | 浙江工业大学 | Extraction method of Mongolian snakegourd effective ingredient |
| CN102068506A (en) * | 2009-11-19 | 2011-05-25 | 孙胜波 | Sterilizing and antimicrobial Chinese medicinal disinfectant |
-
2012
- 2012-12-18 CN CN2012105513692A patent/CN102960371A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1109708A (en) * | 1994-12-28 | 1995-10-11 | 黄可荣 | Plant pesticide composition and preparing process thereof |
| WO2007139382A2 (en) * | 2006-05-29 | 2007-12-06 | Praktijkonderzoek Plant & Omgeving B.V. | Method for controlling a fungal foliar pathogens with an extract from yucca |
| CN101449804A (en) * | 2008-12-23 | 2009-06-10 | 浙江工业大学 | Extraction method of Mongolian snakegourd effective ingredient |
| CN102068506A (en) * | 2009-11-19 | 2011-05-25 | 孙胜波 | Sterilizing and antimicrobial Chinese medicinal disinfectant |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651615A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Biological insecticide containing lycopene and preparation method of biological insecticide |
| CN103651615B (en) * | 2013-12-18 | 2015-08-05 | 青岛百瑞吉生物工程有限公司 | A kind of biological insecticides containing lycopene and preparation method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130313 |