CN102980930A - Preparation method of electric wettability electrode - Google Patents
Preparation method of electric wettability electrode Download PDFInfo
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- CN102980930A CN102980930A CN201210548719XA CN201210548719A CN102980930A CN 102980930 A CN102980930 A CN 102980930A CN 201210548719X A CN201210548719X A CN 201210548719XA CN 201210548719 A CN201210548719 A CN 201210548719A CN 102980930 A CN102980930 A CN 102980930A
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Abstract
The invention discloses a preparation method of an electric wettability electrode. The preparation method comprises the following steps of: 1) loading conductive silver ink, and printing the silver electrode on a PET (Polyethylene Glycol Terephthalate) membrane by using a printer; 2) after the printing, sintering the printed electrode at a temperature of 120-200 DEG C for 3minutes to 30minutes; 3) treating by using ultraviolet/ozone with 20-40mW/cm<2> for 3-10minutes, subsequently washing by using double distilled water, and drying by using nitrogen; and 4) putting the electrode obtained in the step 3 into 1-3mM 1H, 1H, 2H, 2H-perfluor decyl mercaptan ethanol solutions, oscillating for 1.5-5 hours, taking out, washing by using ethanol, drying by using nitrogen and obtaining the electric wettability electrode. The electric wettability electrode prepared by the preparation method has the characteristics of convenience in manufacturing, low cost and long hydrophily maintaining time, can be used for manufacturing electric control valves in a micro-fluid device, and plays a positive role in the popularization and use of a portable micro-fluid analysis method.
Description
Technical field
The present invention relates to the analytical test field, particularly a kind of preparation method of the electric wetting electrode for portable microflow analysis device.
Background technology
Based on the bioanalysis device of microfluid, have the high advantage of high specificity and susceptibility, thereby more welcomed by the people.At present, the microfluid that is used for clinical disease diagnosis exists complex structure, the shortcoming such as expensive, should not move, thereby be not suitable for some places that need to detect such as farm on the spot and use.The special device of the mobile needs of liquid drives in the microfluid, normally adopts exogenous pump (such as micro-injection pump, peristaltic pump and or air driven pump) to provide power for it.Owing to used pump, the volume of checkout equipment increases greatly.Therefore, the scientific research personnel is being engaged in design and the development of pump-free type microfluid always.
Adopting driven by electroosmosis is a method of dealing with problems.Yet the voltage that electroosmotic flow requires is very high, and therefore, this method is in portable, the cheaply mobile seldom employing that detects in establishing.
Use capillary flow to have lot of advantages in microfluid, wherein the most outstanding is that liquid can flow in without the situation of pump, is very beneficial for simplification and the microminiaturization of system.This also is that direction finding flow analysis method can be widely used in the major reason that detects, diagnose.Capillary fluid flows in microfluidic device and is subjected to several factor affecting, comprises design, liquid viscosity and the solid-liquid contact angle of pipeline.Usually the liquid that uses in the analytic process is hydrophilic, and the polymer material that is used for making microfluidic device is hydrophobicity, and how to reduce both contact angles be one of study hotspot of this area to the application surface modification technique.It is the most frequently used hydrophilic method of acquisition that the surface is coated with, and for example, coats cellulose acetate in polyvinyl chloride surface, can increase Capillary Flow.
In addition, strengthen the subregional hydrophobicity of channel middle, flowing of the liquid that can slow down reaches to increase and hatches (such as the combination of antigen and the antibody) time.At present, increase the channel surface hydrophobicity and be used to the interior sample mix of microfluidic channel.
Medium powers on, and wetting (EWOD) is a kind of special phenomenon, and the polarity of hydrophobic medium can be changed when making alive.Teflon is a kind of hydrophobic medium, when powering up, can change polarity, is converted into water wettability, shows as contact angle and diminishes.Liquid except the property effect that is subjected to self, also is subjected to the impact of the contact angle of inner surface of pipeline in flow in capillary tube.So ducted hydrophobicity teflon door can be blocked flowing of capillary liquid in pipe.If teflon is converted into water wettability, capillary liquid recovers to flow.Therefore, in the situation that does not need the movability parts, this electric wetting state teflon can be made the valve of automatically controlled property.There is the scholar to studies show that, utilizes electric wetting phenomena can be made into valve, be used for the liquid that the siphon of control capillary drives.In the test, when elasticity dimethyl silicone polymer on glass was induced into water wettability, the capillary liquid in pipe could flow.Yet this finishing is not suitable for being prepared into commercial material.
Accordingly, the present invention discloses the method for the wet valve of a kind of electricity and preparation.Compare with elasticity poly dimethyl silica, this method have preparation easily, the characteristics of low-cost, high benefit, can keep water wettability for a long time.
Summary of the invention
The technical matters that solves: the objective of the invention is to prepare the electric wetting state electrode that is suitable for using on the microfluidic device, behind the on-load voltage, the polarity of electrode transfers to hydrophilicly by hydrophobic, reaches flowing and blocking-up of liquid in the control microfluid.
Technical scheme: a kind of preparation method of electric wetting state electrode, step is: 1). loads the conductive silver ink, prints silver electrode with printer at polyethylene terephthalate (PET) film; 2). printed rear 10～90 minutes, with the printed electrode sintering, 120～200 ℃ of temperature, time 3min～30min; 3). process with UV/ozone: 20～40mW/cm
2, time 3～10min, then distilled water cleans, nitrogen drying; 4). step 3 the electrode obtained is placed 1～3mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration, 1.5～5 hours, take out, ethanol cleans, and gets electric wetting state electrode behind the nitrogen drying.
Beneficial effect: existing microfluidic device adopts the mechanicalness valve usually, and volume is larger, carries difficulty, is not easy to detect on the spot.The electric wetting electrode of the present invention's preparation has characteristics easy to make, that cost is low, the maintenance water wettability time is long, can be used for making the electric control valve in the microfluidic device, for the positive role of having promoted the use of of portable microfluid analysis method.
Description of drawings
Fig. 1 is the silver electrode that prints on the PET film;
Fig. 2 is that the electrode that utilizes the present invention to make prepares the electrically-controlled valve synoptic diagram, and A is microfluid inner capillary tube stream among the figure, and B is common silver electrode, and C is electric wetting state electrode.
Embodiment
Adopt and measure the performance that contact angle is estimated the electric wetting state electrode of the present invention's preparation.
The contact angle of electrode: the contact angle of detecting electrode and pure water on angular instrument.
Electrode contact angle under electricity is wetting: the liquid of employing is 3 μ L 1M KCl solution, places diameter 25 μ m gold threads in the KCl drop, loads 4V voltage, measures contact angle and the variation relation of time.
Following embodiment does not limit technical scheme of the present invention in any form, and every employing is equal to replaces or technical scheme that the mode of equivalent transformation obtains all drops on protection scope of the present invention.
Embodiment 1
Adopt HP Photosmart C-4580 ink-jet printer, remove the ink in the print cartridge, clean up drying.((Sigma-Aldrich Corp., St.Louis, MO, USA) puts into print cartridge to the conductive silver ink.Adopt AutoCAD Software for Design electrode, and print.Before printing, be sprayed to PET(3M, St.Paul, MN, USA with the ethanol of 70%wt) on the film, then clean nitrogen drying with distilled water.Adopt high-quality (1200Dpi) to print, obtain silver electrode, as shown in Figure 1.
Embodiment 2
1) with embodiment 1 print electrode 10min after, with the printed electrode sintering, 190 ℃ of temperature, time 3min;
2) process with UV/ozone: 20mW/cm
2, time 5min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 3mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 4h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 140 ° for the contact angle of pure water.Contact angle to KCl is 106 °, loads 4V voltage, and behind the 15s, contact angle is 28 °, and behind the 60s, contact angle is 22 °.
Embodiment 3
1) with embodiment 1 print electrode 15min after, with the printed electrode sintering, 175 ℃ of temperature, time 5min;
2) process with UV/ozone: 28mW/cm
2, time 6min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 2mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 3h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 148 ° for the contact angle of pure water.Contact angle to KCl is 112 °, loads 4V voltage, and behind the 15s, contact angle is 29 °, and behind the 60s, contact angle is 23 °.
Embodiment 4
1) with embodiment 1 print electrode 90min after, with the printed electrode sintering, 150 ℃ of temperature, time 30;
2) process with UV/ozone: 40mW/cm
2, time 10min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 2mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 1.5h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 142 ° for the contact angle of pure water.Contact angle to KCl is 108 °, loads 4V voltage, and behind the 15s, contact angle is 26 °, and behind the 60s, contact angle is 21 °.
Embodiment 5
1) with embodiment 1 print electrode 75min after, with the printed electrode sintering, 160 ℃ of temperature, time 20;
2) process with UV/ozone: 28mW/cm
2, time 4min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 4mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 2h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 139 ° for the contact angle of pure water.Contact angle to KCl is 105 °, loads 4V voltage, and behind the 15s, contact angle is 27 °, and behind the 60s, contact angle is 25 °.
Embodiment 6
1) with embodiment 1 print electrode 30min after, with the printed electrode sintering, 120 ℃ of temperature, time 5;
2) process with UV/ozone: 28mW/cm
2, time 7min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 2.5mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 5h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 145 ° for the contact angle of pure water.Contact angle to KCl is 111 °, loads 4V voltage, and behind the 15s, contact angle is 26 °, and behind the 60s, contact angle is 22 °.
Embodiment 7
1) with embodiment 1 print electrode 60min after, with the printed electrode sintering, 130 ℃ of temperature, time 10;
2) process with UV/ozone: 20mW/cm
2, time 3min.Then distilled water cleans, nitrogen drying;
3) electrode is placed 1.5mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration 3h takes out, and ethanol cleans.Nitrogen drying.
The electrode that obtains be 150 ° for the contact angle of pure water.Contact angle to KCl is 115 °, loads 4V voltage, and behind the 15s, contact angle is 29 °, and behind the 60s, contact angle is 23 °.
Use the electrode obtained and prepare electrically-controlled valve, be used for the microfluid analysis device, by changing voltage, realize capillaceous flowing and blocking-up.As shown in Figure 2.Microfluidic device has two silver electrodes, and left side (upper strata) is common silver electrode, is water wettability, and right side (lower floor) is the prepared electric wetting state electrode of the present invention.Not during on-load voltage, liquid barrier in the kapillary.When loading 4V voltage, liquid flow is unobstructed in the microfluid pipeline.
Claims (1)
1. the preparation method of an electric wetting state electrode is characterized in that step is: 1). load the conductive silver ink, print silver electrode with printer at polyethylene terephthalate (PET) film; 2). printed rear 10～90 minutes, with the printed electrode sintering, 120～200 ℃ of temperature, time 3min～30min; 3). process with UV/ozone: 20～40mW/cm
2, time 3～10min, then distilled water cleans, nitrogen drying; 4). step 3 the electrode obtained is placed 1～3mM 1H, 1H, 2H, the ethanolic solution of 2H-perfluor decyl mercaptan, vibration, 1.5～5 hours, take out, ethanol cleans, and gets electric wetting state electrode behind the nitrogen drying.
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| CN201210548719.XA CN102980930B (en) | 2012-12-17 | 2012-12-17 | Preparation method of electric wettability electrode |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109806803A (en) * | 2019-02-28 | 2019-05-28 | 南京理工大学 | A kind of microfluid mixing device and its control method with electrowetting threshold gate |
| CN109908981A (en) * | 2019-02-28 | 2019-06-21 | 南京理工大学 | Electrowetting valve and its control method in a kind of micro-fluidic paper chip |
| CN110662965A (en) * | 2017-04-07 | 2020-01-07 | 脱其泰有限责任公司 | Flow sensing device having at least one electrically actuated fluid flow control valve and related methods |
| US11445577B2 (en) | 2016-06-17 | 2022-09-13 | Swansea University | Glass laminate structure |
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| CN1587023A (en) * | 2004-09-30 | 2005-03-02 | 清华大学 | Micro liquid drop driver based on power-on wetting of medium layer |
| CN101287845A (en) * | 2005-05-11 | 2008-10-15 | 先进液体逻辑公司 | Method and device for conducting biochemical or chemical reactions at multiple temperatures |
| CN102500436A (en) * | 2011-09-28 | 2012-06-20 | 复旦大学 | Single-sided two-dimensional driving digital microfluidic chip based on electrowetting |
| CN102698822A (en) * | 2012-06-11 | 2012-10-03 | 复旦大学 | Universal electrode structure based on digital microfluidic chip |
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2012
- 2012-12-17 CN CN201210548719.XA patent/CN102980930B/en not_active Expired - Fee Related
Patent Citations (4)
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| CN1587023A (en) * | 2004-09-30 | 2005-03-02 | 清华大学 | Micro liquid drop driver based on power-on wetting of medium layer |
| CN101287845A (en) * | 2005-05-11 | 2008-10-15 | 先进液体逻辑公司 | Method and device for conducting biochemical or chemical reactions at multiple temperatures |
| CN102500436A (en) * | 2011-09-28 | 2012-06-20 | 复旦大学 | Single-sided two-dimensional driving digital microfluidic chip based on electrowetting |
| CN102698822A (en) * | 2012-06-11 | 2012-10-03 | 复旦大学 | Universal electrode structure based on digital microfluidic chip |
Non-Patent Citations (1)
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| 李伟，李梅，路庆华: "离子液体在超疏水银膜表面的电润湿性能", 《上海交通大学学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11445577B2 (en) | 2016-06-17 | 2022-09-13 | Swansea University | Glass laminate structure |
| CN110662965A (en) * | 2017-04-07 | 2020-01-07 | 脱其泰有限责任公司 | Flow sensing device having at least one electrically actuated fluid flow control valve and related methods |
| CN110662965B (en) * | 2017-04-07 | 2023-09-12 | 脱其泰有限责任公司 | Flow sensing apparatus having at least one electrically actuated fluid flow control valve and related methods |
| CN109806803A (en) * | 2019-02-28 | 2019-05-28 | 南京理工大学 | A kind of microfluid mixing device and its control method with electrowetting threshold gate |
| CN109908981A (en) * | 2019-02-28 | 2019-06-21 | 南京理工大学 | Electrowetting valve and its control method in a kind of micro-fluidic paper chip |
| CN109806803B (en) * | 2019-02-28 | 2022-02-18 | 南京理工大学 | Microfluid mixing device with electrowetting valve and control method thereof |
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| CN102980930B (en) | 2014-11-05 |
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