CN102960539A - High-protein microbiological feed and preparation method thereof - Google Patents
High-protein microbiological feed and preparation method thereof Download PDFInfo
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Abstract
The invention provides a high-protein microbiological feed and a preparation method of the high-protein microbiological feed. Dross is one of products produced in the process of floating and clarifying the mixed juice during sugar preparing by the sugarcane, and is rich in various organic non-sugar components; the dross is always considered as impurities and mixed in the mud juice by a sugar plant, and finally becomes lime sludge to cause waste. The preparation method provided by the invention comprises the following steps of taking the dross in the crane sugar plant as the raw material, and taking a non-protein nitrogen source, phosphate and magnesium salt and the like as the auxiliary materials; mixing the raw material and the auxiliary materials; sterilizing; adding beneficial zymocyte preparation; and fermenting at the aerobic condition so as to obtain the high-protein microbiological feed. By adopting the preparation method provided by the invention, the problems that a large quantity of dross is in the crane sugar plant and is adhered and hardly processed, and the loss of the sugar is serious can be solved well; and the current situation that the protein feed resources are in a shortage can be relieved. According to the preparation method, the cost is low, the period is short, the production technology is simple, the limitation from the field and equipment is less, and the quality and the yield of the high-protein microbiological feed are high, and the preparation method plays an important role in popularization and application of the mixed juice floating and clarifying technology in sugar plants.
Description
Technical field
The invention belongs to resource environment and biological feedstuff field, be specially high protein microbiological feed of a kind of preparation take the cane sugar factory scum silica frost as primary raw material and preparation method thereof.
Background technology
Scum silica frost is the class accessory substance in the cane sugar manufacture production process.Because the hysteresis of correlative study, and the problems such as the viscosity of scum silica frost own is large, high humidity, it is impurity that scum silica frost is looked in sugar refinery all the time.In order to reclaim as far as possible the sugar in the scum silica frost, mix whole scum silica frost usually in sugar refinery with whole mud juices of settler, with vacuum filter without cloth or Filter Press, finally become filter mud again.
Improving the comprehensive utilization ratio of cane sugar factory mixed juice scum silica frost and the quality of commerieal sugar is the two large technical bottlenecks that sugar industry faces for a long time.Along with improving constantly and the day by day enhancing of environmental protection consciousness of commerieal sugar quality requirement, sugar enterprise is faced with and promotes the dual-pressure that production technology and byproduct comprehensive utilize.
For adapting to new production and product demand, mixed juice air float technique (mixed juice come-up technique) is suggested and is applied in the production, thereby this technique alleviates follow-up workshop section removal of impurities load and reduces the chemical assistant consumption by in advance the inorganic non-sugar in the mixed juice being separated, and changes the passive situation that the sugaring old technology " pollutes first again and administers " from the source.Simultaneously, the mixed juice floating method has also been avoided sugarcane juice high-temperature heating so that contained non-sugar impurities sex change or the decomposition such as protein, cerosin, sugarcane fat and pectin of sugarcane itself.From the mixed juice scum silica frost, extract or to utilize the biological active matters such as high-quality protein, cerosin, sugarcane fat and pectin to extract from filter mud more economical, feasible.At present, the research report of the comprehensive utilization of cane sugar factory mixed juice scum silica frost is detected in the scientific achievement of Chen Shan seminar of Guangxi University.This seminar has carried out comparatively full and accurate analysis to the contained various nutritional labelings of mixed juice scum silica frost, and extract the polyphenoils such as purification of flavone, Polyphenols take scum silica frost as raw material, and the testing identity extract has preferably anti-oxidation function, and this is indicating that scum silica frost might become raw material sources of feed, food service industry.The patent (CN200410012967.8) of this seminar existing " utilizing sugarcane fat and chlorophyll in the scum silica frost of solvent concurrent extracting sugar refinery " aspect separation and purification.
Protein resource is basis of feed industry, and at present protein feed shortage has become the outstanding problem that feedstuff industry develops.At present the internal feed demand is 1.5 hundred million ton/years, needs approximately 3,750 ten thousand tons of protein raw materials, wherein 70% dependence on import.Moreover, along with the development of feed industry, the demand of protein raw material also increases in proportion.Utilize existing biotechnology, originate take the industrial or agricultural accessory substance as major protein and can develop the multiple proteins feedstuff, can turn waste into wealth, environmental contamination reduction can be alleviated again feed industry to the dependence of grain, promotes the development of aquaculture.Chinese scholar Cai Jun has studied take brewex's grains as primary raw material, by mixed fermentation, improves the technique of its protein content, and has obtained good effect; Hao Junyuan etc. have invented a kind of method of utilizing waste potato dregs to prepare animal feeding-stuff containing somatic protein, waste residue after the method is processed with potato, add again and carry out the microbial fermentation cultivation after the raw materials such as zein, ammonium sulfate mix, utilize the microbial bacteria body protein to improve forage protein content; US Experts SMatsuoka utilizes Production by Microorganism Fermentation bagasse feed equally, and the method can make bagasse become the high protein feed of a kind of ruminant digestibility.At present, report out that both at home and abroad utilizing this technology to produce the used proteinogen material of high protein feed also has citrus pulp, bean dregs, bean curd stick slag, megasse, acid-sludge etc., has no the report that utilizes sugar refinery scum silica frost to be converted into microbial protein feed.
Guangxi is maximum sucrose producing region, the whole nation, and cane sugar industry is the pillar industry in Guangxi, but relatively external whole backwardness of production technology, particularly is short of aspect utilizing two at sugar products deep processing and byproduct comprehensive.The present invention is take cane sugar factory mixed juice scum silica frost as raw material, use the recycling that single cell protein fermenting organism technology realizes scum silica frost, not only can promote the mixed juice floating method in the promotion and application in sugar refinery, also can be China's feedstuff industry a kind of new feedstuff that is rich in high protein is provided, using value is large, and market prospects are wide.
Summary of the invention
The present invention is directed to the scum silica frost that produces in the Present Domestic cane sugar manufacture process mixed juice come-up clarification process and affect sucrose yield and quality, scum silica frost is difficult to the problem of recycling, and the present situation of domestic protein feed resources shortage, utilize sugar refinery scum silica frost as raw material, provide a kind of by biotechnology with high protein microbiological feed of sugar refinery scum silica frost fermenting and producing and preparation method thereof, the problem that is solving sugar refinery accessory substance and be difficult to process, when improving the sucrose quality, widen source and the production ways of protein feed, serve sugar industry, environmental protection industry (epi) and feed industry.
In order to realize described purpose, the present invention adopts the biotechnology principle, utilize beneficial microbe bacterial classification or strain combination can decompose, utilize the characteristic of the macromolecule carbon source materials such as cellulose in the scum silica frost, hemicellulose, lignin, sugarcane fat, starch, and under suitable non protein nitrogen, inorganic salts, temperature and humidity conditions cooperate, microorganism fungus kind cell fast breeding, be converted into the microbial bacteria body protein, thereby increased total protein content and the amino acid content of scum silica frost.
The present invention is achieved by the following technical solutions:
A kind of high protein microbiological feed, described high protein microbiological feed is to add useful fermenting agent fermentation to make in mixed material, described mixed material is comprised of the material of following weight part ratio: 30～70 parts of cane sugar factory scum silica frost (in the over dry material), 0.1～4 part of non protein nitrogen, 0.1～3 part of phosphate, 0.1～3 part of magnesium salts, 30～65 parts in water, regulating the pH value is 4.5～7.5, add the useful fermenting agent that 2～20% usefulness beneficial microbe bacterial classifications account for described mixed material gross weight are made, effective total viable count of described useful fermenting agent is not less than 100,000,000/g, through mixing, fermented and cultured, dry, pulverize, get the high protein microbiological feed.
Above-mentioned high protein microbiological feed comprises the composition of following percentage by weight: crude protein 〉=38%, total amino acid content 〉=20%, crude fat 7～15%, crude fibre 7～18%.
Described beneficial microbe bacterial classification is that long handle wood is mould, any one or more than one combination in the aspergillus niger, lonely humicola lanuginosa, penicillium funiculosum, geotrichum candidum, candida utili, brewer's yeast, fission yeast, candida tropicalis, cultivates by the expansion of bacterial classification and is prepared into liquid bacterial agent or solid fungicide.
Long handle wood is mould, and Latin is called Trichoderma longibrachiatum, trichoderma a kind of, and feed (additive) production is used bacterial classification.
Aspergillus niger, Latin is called Aspergillus niger, aspergillus a kind of, feed (additive) production is used bacterial classification.
Lonely humicola lanuginosa, Latin be called Humicola insolens, Humicola a kind of, feed (additive) production bacterial classification.
Penicillium funiculosum, Latin is called Penicillium funiculosum, Penicillium a kind of, feed (additive) production is used bacterial classification.
Geotrichum candidum, another name: newborn ovum spore is mould, and Latin be called Geotrichum candidum Link, Geotrichum a kind of, feed (additive) production bacterial classification.
Candida utili, another name: produce protein torula or edible torula, Latin be called candida utilis, candida a kind of, feed (additive) production bacterial classification.
Brewer's yeast, another name: saccharomyces cerevisiae, saccharomyces sake, Ka Ersibai yeast, Latin be called Saccharomycse cerevisiae Hansen, saccharomyces a kind of, feed (additive) production bacterial classification.
Fission yeast, Latin is called Schizosaccharomyces, Schizosaccharomyces a kind of, feed (additive) production is used bacterial classification.
Candida tropicalis, Latin is called Candida tropicalis, candida a kind of, feed (additive) production is used bacterial classification.
Described auxiliary material non protein nitrogen is any one or more than one combination in urea, ammonium chloride, ammonium sulfate, carbonic hydroammonium, ammonium dihydrogen phosphate (ADP), diammonium hydrogen phosphate, urea phosphate, sodium nitrate, the potassium nitrate, and these auxiliary materials are that food additives or feed addictive allow to use kind.
Urea, another name carbonyl diamide, phosphoamide, urea, CAS number: 57-13-6, chemical formula: CON
2H
4, CO (NH
2)
2Or CN
2H
4O, molecular weight: 60.05, outward appearance is white crystal or powder.
Ammonium chloride is commonly called as: electric salt, electric medicinal powder, smart, the sal ammoniac of salt, CAS number: 12125-02-9, chemical formula: NH
4Cl, molecular weight: 53.49, colourless cubic crystal or white crystalline powder.
Ammonium sulfate, CAS number: 7783-20-2, chemical formula: (NH
4)
2SO
4, molecular weight: 132.13, sterling is colourless rhomboidal crystal, industrial goods are that white is to faint yellow crystalline solid.Odorlessness.
Carbonic hydroammonium, Chinese another name: ammonium bicarbonate, ammonium bicarbonate, carbon ammonium, CAS number: 1066-33-7, chemical formula: NH
4HCO
3, molecular weight: 79.06, colourless or white prismatic crystallization.Little have an ammonia odor.
Ammonium dihydrogen phosphate (ADP) is called again MAP, CAS number: 7722-76-1, chemical formula: NH
4H
2PO
4, molecular weight: 115.03, white crystalline powder.
Diammonium hydrogen phosphate, Chinese another name: Diammonium phosphate (DAP); Industrial phosphoric acid two ammoniums; Diammonium hydrogen phosphate (technical grade); Ammonium hydrogen phosphate, CAS number: 7783-28-0, chemical formula: (NH
4)
2HPO
4, molecular weight: 132.06, white crystal or powder.
Urea phosphate, another name: urea phosphate, CAS number: 4861-19-2, chemical formula: CH
7N
2O
5P, molecular weight: 158.05, proterties water white transparency prism-shaped crystal.
Sodium nitrate, CAS number: 7631-99-4, chemical formula: NaNO
3, molecular weight: 85.00, water white transparency or the yellow rhomboidan of white little band.
Potassium nitrate is commonly called as nitre or native nitre, CAS number: 7757-79-1, chemical formula: KNO
3, molecular weight: 101.10, water white transparency prism-shaped or white particle or crystalline powder.
Described auxiliary material phosphate is any one or more than one combination in tripotassium phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, tertiary sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, diammonium hydrogen phosphate, ammonium hydrogen phosphate, the sodium pyrophosphate, and these auxiliary materials are that food additives or feed addictive allow to use kind.
Tripotassium phosphate, another name: potassium phosphate; Potassium phosphate (three water); Potassium orthophosphate; Anhydrous phosphoric acid potassium, CAS number: 7778-53-2; 16068-46-5, chemical formula: K
3PO
4, molecular weight: 212.26, colourless orthorhombic system crystallization or white crystalline powder.
Dipotassium hydrogen phosphate, another name: dikalium phosphate, CAS number: 7758-11-4, chemical formula: K
2HPO
43H
2O, molecular weight: 228.22, outward appearance is white crystals or amorphous white powder, and is soluble in water.
Potassium dihydrogen phosphate, CAS number: 7778-77-0, chemical formula: KH
2PO
4, molecular weight: 136.09, be colourless crystallization or white particle sprills.
Tertiary sodium phosphate, another name: anhydrous sodium phosphate, sodium orthophosphate, the commercial sodium phosphate that claims again.CAS number: 10101-89-0, chemical formula: Na
3PO
4.12H
2O, molecular weight: 380.20, colourless needle-like hexagonal crystal; CAS number: 7601-54-9, chemical formula: Na
3PO
4, molecular weight: 164, colourless needle-like hexagonal crystal.
Sodium hydrogen phosphate, another name: ten phosphate dihydrate disodiums; Disodium hydrogen phosphate, CAS number: 7558-79-4, chemical formula: Na
2HPO
412H
2O, molecular weight: 358.14.Water white transparency monoclinic system prismatic crystal.
Sodium dihydrogen phosphate, another name: monosodium phosphate, Sodium Dihydrogen Phosphate, two hypophosphite monohydrate sodium dihydrogens, CAS number: 13472-35-0, chemical formula: NaH
2PO
42H
2O, molecular weight: 156.01, colourless iris crystallization, slightly hygroscopicity.
Sodium pyrophosphate, another name: tetrasodium phosphate; Anhydrous sodium pyrophosphate, CAS number: 7722-88-5, chemical formula: Na
4P
2O
7, molecular weight: 265.9, white powdery or crystallization.
Described auxiliary material magnesium salts is any one or the two kinds of combinations in magnesium sulfate, the magnesium chloride, and these auxiliary materials are that food additives or feed addictive allow to use kind.
Magnesium sulfate, another name: epsom salt has another name called magnesium sulphate, sal amarum, epsomite, Epsom salts, CAS number: 10034-99-8, chemical formula: MgSO
4(or MgSO
47H
2O), molecular weight 120.37 or 246.47 belongs to orthorhombic system, is four jiaos of granular or rhomboidans, and colourless, transparent, aggregate is white, rose-colored or green glass gloss.That shape has is fibrous, needle-like, granular or powder.Odorless, refrigerant, bitter saline taste is arranged, relative density 1.67～1.71.
Magnesium chloride, another name: magnesium chloride (hexahydrate), magnesium chloride hexahydrate, CAS number: 7791-18-6, chemical formula: MgCl
2(or MgCl
26H
2O), molecular weight: 95.21 or 203.30, colourless crystallization, easily deliquescence is soluble in water, can be dissolved in alcohol.
Described cane sugar factory scum silica frost derives from the cane sugar factory mixed juice and processes the product that obtains through the come-up clarification process, contains the nutriments such as carbohydrate, fat and a small amount of protein, mineral matter.For feeding intake accurately, cane sugar factory scum silica frost of the present invention calculates with the over dry material.The over dry material refers to material is placed in the baking oven, keeps being dried to the material that constant weight obtains under the constant condition of physical property.The computing formula of over dry material of the present invention is:
m
Do=m
Wet* (1-W)
W in the formula---moisture content (%)
m
Wet---wet cane sugar factory scum silica frost quality (g)
m
Do---over dry cane sugar factory scum silica frost quality (g)
The assay method of cane sugar factory scum silica frost moisture content W of the present invention is: evenly get cane sugar factory scum silica frost 5～10g, be ground into the particle that diameter is no more than 3mm, put in the baking oven and under 100～105 ℃, be dried to double difference of weighing below 0.3mg, calculate moisture content according to the weight that subtracts mistake, computing formula is:
The preparation method of the above high protein microbiological feed may further comprise the steps:
1. the preparation of useful fermenting agent: the beneficial microbe bacterial classification is enlarged cultivation, make useful fermenting agent;
2. cane sugar factory scum silica frost preliminary treatment: with fresh cane sugar refinery dross dehydration, obtain water content and be 40%～65% wet scum silica frost, pulverize, for subsequent use; Or with fresh cane sugar refinery dross dehydration, drying, obtain water content and be lower than 20% dry dross, pulverize, for subsequent use;
3. prepare burden: take by weighing mixed material by following weight part ratio: cane sugar factory scum silica frost 30～70 parts of (in the over dry material), 0.1～4 part of non protein nitrogen, 0.1～3 part of phosphate, 0.1～3 part of magnesium salts, 30～65 parts in water; Place proportion container to mix mixed material, regulating pH value is 4.5～7.5, forms fermentation materials;
4. sterilization: fermentation materials is moved into disinfection tank, use steam in 100～121 ℃ of sterilization 15～20min;
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to below 40 ℃, add the useful fermenting agent of mixed material gross weight 2～20%, and abundant mixing, under aerobic condition, 20～35 ℃ of fermentation 2～6d;
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Described useful fermenting agent contains that long handle wood is mould, any one or more than one beneficial microbe strain combination in the aspergillus niger, lonely humicola lanuginosa, penicillium funiculosum, geotrichum candidum, candida utili, fission yeast, candida tropicalis.
The preparation method of the above high protein microbiological feed, described useful fermenting agent comprises liquid bacterial agent and solid fungicide, it is in the following ways preparation respectively:
The preparation of liquid bacterial agent: with in the beneficial microbe bacterial classification any one or more than one be inoculated in the scale-up medium, under aerobic condition, enlarge to cultivate 1～5d for 25～30 ℃, the living bacteria count of gained microbial inoculum is greater than 100,000,000/g, the miscellaneous bacteria rate is lower than 20%; Described scale-up medium prescription is comprised of the material of following weight part ratio: 16～20 parts in molasses, 75～85 parts in water, 0.3～0.8 part in urea, 0.1～0.3 part of potassium dihydrogen phosphate, and regulating the pH value is 5.0～6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product;
The preparation of solid fungicide: with in the beneficial microbe bacterial classification any one or more than one be inoculated in and enlarge in the culture medium, under aerobic condition, 25～30 ℃ enlarge cultivation 1～5d, culture in temperature≤50 ℃, constant pressure and dry or vacuum freeze-drying, grind or pulverize, mix, the living bacteria count of gained microbial inoculum is greater than 200,000,000/g, and the miscellaneous bacteria rate is lower than 30%; Described expansion culture medium prescription is comprised of the material of following weight part ratio: 45～50 parts in wheat bran, 1～3 part of sucrose, 45～55 parts in water, 0.1～0.5 part of diammonium hydrogen phosphate, regulating the pH value is 5.0～6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Formula Design of the present invention is reasonable, preparation method's science, and cost is low, can be used as the feed of the letting animals feeds such as pig, ox, sheep, chicken.Feed after the fermentation is fragrant, and palatability is better, can stimulate livestock appetite, increases feed intake; Nutritious, protein content is higher, crude protein 〉=38%, and contain 18～20 seed amino acids, and total amino acid content 〉=20%, other contains multivitamin, and the digestibility of feed improves, and daily gain and meat feed ratio increase; Contain multiple probio, can improve micro-ecological environment in the letting animals feed body, suppress the growth of pathogenic bacteria in the body, improve physique and resistance against diseases.
Compared with prior art, the invention has the beneficial effects as follows:
1. prepare the high protein microbiological feed take sugar refinery scum silica frost as primary raw material, can solve the sugar plant pollution problem, can expand again new source and the production ways of protein feed;
Raw material be easy to get, inexpensive, technical recipe is simple, normal temperature and pressure fermentation is not high to equipment and site requirements, can utilize sugar refinery equipment component and resource, has the few advantage of investment;
3. the present invention's beneficial microbe bacterial classification metabolism of selecting is vigorous, and growth rate is fast, and output capacity is high, and economic benefit and social benefit are obvious.
The specific embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited to the scope that embodiment represents.
One, the preparation of scale-up medium
Scale-up medium prescription 1: take by weighing material by following weight: molasses 2Kg, water 8.5Kg, urea 0.08Kg, potassium dihydrogen phosphate 0.03Kg, regulating the pH value is 5.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Scale-up medium prescription 2: take by weighing material by following weight: molasses 1.6Kg, water 7.5Kg, urea 0.01Kg, potassium dihydrogen phosphate 0.01Kg, regulating the pH value is 6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Scale-up medium prescription 3: take by weighing material by following weight: molasses 1.83Kg, water 8.1Kg, urea 0.05Kg, potassium dihydrogen phosphate 0.02Kg, regulating the pH value is 5.5,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Two, enlarge the preparation of culture medium
Enlarge culture medium prescription 1: take by weighing material by following weight: wheat bran 4.5Kg, sucrose 0.1Kg, water 4.5Kg, diammonium hydrogen phosphate 0.01Kg, regulating the pH value is 5.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Enlarge culture medium prescription 2: take by weighing material by following weight: wheat bran 5.0Kg, sucrose 0.3Kg, water 5.5Kg, diammonium hydrogen phosphate 0.05Kg, regulating the pH value is 6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Enlarge culture medium prescription 3: take by weighing material by following weight: wheat bran 4.77Kg, sucrose 0.2Kg, water 5.0Kg, diammonium hydrogen phosphate 0.03Kg, regulating the pH value is 5.5,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
Three, the preparation method of high protein microbiological feed
Embodiment 1
1. the preparation of useful fermenting agent: mould being inoculated into respectively with candida utili of long handle wood enlarged in the culture medium prescription 1, under 25～30 ℃, aerobic condition, the mould cultivation of long handle wood 5 days, candida utili was cultivated 1 day, culture grinds after 40 ℃ of normal pressure forced air dryings evenly, the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 50% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 96.8kg(contains over dry material 48.4kg), ammonium chloride 0.5kg, dipotassium hydrogen phosphate 0.2kg, magnesium sulfate 0.1kg, water 3.2kg places proportion container to mix, and with being 6.5 with 1mol/L NaOH pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 105 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add the 1.0kg long handle mould solid fungicide of wood and 1.0kg candida utili solid fungicide, and abundant mixing, under aerobic condition, 25 ℃ of fermentation 6d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 2
1. the preparation of useful fermenting agent: with the aspergillus niger kind to enlarging in the culture medium prescription 2, under 25～30 ℃, aerobic condition, cultivated 4 days, culture grinds after 38 ℃ of normal pressure forced air dryings evenly, and the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%; Brewer's yeast is inoculated in the scale-up medium 1, cultivated 2 days under 25～30 ℃, aerobic condition, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 40% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 90kg(contains over dry material 36kg), ammonium sulfate 1.2kg, sodium hydrogen phosphate 0.3kg, magnesium chloride 0.2kg, place proportion container to mix, and with being 7.5 with 1mol/L NaOH pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use water vapour in 115 ℃ of sterilization 18min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 28 ℃, add 1.0kg aspergillus niger solid fungicide and 7.3kg saccharomyces cerevisiae liquid, and abundant mixing, under aerobic condition, 29 ℃ of fermentation 4d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 3
1. the preparation of useful fermenting agent: lonely humicola lanuginosa and fission yeast are inoculated into respectively in the scale-up medium prescription 3, under 25～30 ℃, aerobic condition, lonely humicola lanuginosa was cultivated 4 days, fission yeast was cultivated 1.5 days, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 10% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 55kg(contains over dry material 49.5kg), urea phosphate 1.5kg, sodium dihydrogen phosphate 0.4kg, magnesium sulfate 0.1kg, water 29kg places proportion container to mix, and with being 6.8 with 1mol/L NaOH pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 121 ℃ of sterilization 15min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 35 ℃, add 5.0kg lonely detritus mould liquid and 12.2kg Schizosaccharomyces liquid, and abundant mixing, under aerobic condition, 35 ℃ of fermentation 4d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 4
1. the preparation of useful fermenting agent: penicillium funiculosum and candida tropicalis are inoculated into respectively in the scale-up medium prescription 2, under 25～30 ℃, aerobic condition, penicillium funiculosum was cultivated 4 days, candida tropicalis was cultivated 1.5 days, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 8% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 50kg(contains over dry material 46kg), urea 1.8kg, ammonium hydrogen phosphate 1.3kg, magnesium chloride 1.2kg, water 36.7kg places proportion container to mix, and with being 6.0 with 1mol/L hydrochloric acid pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 105 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 4.0kg penicillium funiculosum liquid and 5.0kg Candida tropicalis liquid, and abundant mixing, under aerobic condition, 26 ℃ of fermented and cultured 5d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 5
1. the preparation of useful fermenting agent: candida tropicalis is inoculated in the expansion culture medium prescription 3, under 25～30 ℃, aerobic condition, cultivated 2 days, culture grinds behind-40 ℃ of vacuum freeze-dryings evenly, and the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 60% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 87kg(contains over dry material 34.8kg), ammonium dihydrogen phosphate (ADP) 0.6kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.1kg, water 2.0kg places proportion container to mix, and with being 5.5 with 1mol/L hydrochloric acid pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use water vapour in 100 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 29 ℃, add 10kg candida tropicalis solid fungicide, and abundant mixing, under aerobic condition, 24 ℃ of fermentation 2.5d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 6
1. the preparation of useful fermenting agent: fission yeast is inoculated in the scale-up medium prescription 2, cultivated 2 days under 25～30 ℃, aerobic condition, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 20% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 60kg(contains over dry material 48kg), carbonic hydroammonium 0.8kg, tertiary sodium phosphate 0.5kg, magnesium chloride 0.2kg, water 40kg places proportion container to mix, and with being 6.5 with 1mol/L sodium hydroxide solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 115 ℃ of sterilization 15min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 26 ℃, add 7.5kg Schizosaccharomyces liquid, and abundant mixing, under aerobic condition, 20 ℃ of fermentation 2d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 7
1. the preparation of useful fermenting agent: geotrichum candidum is inoculated in the scale-up medium prescription 3, cultivated 2 days under 25～30 ℃, aerobic condition, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 12% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 70kg(contains over dry material 61.6kg), diammonium hydrogen phosphate 2.2kg, tripotassium phosphate 1.5kg, magnesium sulfate 0.3kg, 21.6kg add water, place proportion container to mix, and with being 7.2 with 1mol/L sodium hydroxide solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 115 ℃ of sterilization 28min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 5.0kg Geotrichum liquid, and abundant mixing, under aerobic condition, 24 ℃ of fermentation 3d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 8
1. the preparation of useful fermenting agent: mould and brewer's yeast is inoculated into respectively in the scale-up medium prescription 2 with long handle wood, under 25～30 ℃, aerobic condition, the mould cultivation of long handle wood 5 days, brewer's yeast was cultivated 1.5 days, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 10% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 55kg(contains over dry material 49.5kg), sodium nitrate 1.5kg, sodium pyrophosphate 0.5kg, magnesium sulfate 0.2kg, water 33.8kg places proportion container to mix, and with being 4.5 with 1mol/L hydrochloric acid solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use water vapour in 105 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 32 ℃, add 4.0kg long handle Trichoderma liquid and 5.0kg saccharomyces cerevisiae liquid, and abundant mixing, under aerobic condition, 26 ℃ of fermented and cultured 4d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 9
1. the preparation of useful fermenting agent: aspergillus niger and candida utili are inoculated into respectively in the expansion culture medium prescription 3, under 25～30 ℃, aerobic condition, aspergillus niger was cultivated 4 days, candida utili was cultivated 2 days, culture grinds behind-45 ℃ of vacuum freeze-dryings evenly, the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 65% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 85.7kg(contains over dry material 30kg), potassium nitrate 1.2kg, sodium dihydrogen phosphate 0.6kg, 0.2kg magnesium sulfate, place proportion container to mix, and with being 7.0 with 1mol/L sodium hydroxide solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 105 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 3.0kg aspergillus niger solid fungicide and 5.0kg candida utili solid fungicide, and abundant mixing, under aerobic condition, 24 ℃ of fermentation 5d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 10
1. the preparation of useful fermenting agent: lonely humicola lanuginosa and brewer's yeast are inoculated into respectively in the expansion culture medium prescription 3, under 25～30 ℃, aerobic condition, lonely humicola lanuginosa was cultivated 4 days, brewer's yeast was cultivated 1.5 days, culture grinds after 38 ℃ of normal pressure forced air dryings evenly, the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 55% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 88kg(contains over dry material 39.6kg), ammonium sulfate 0.8kg, potassium dihydrogen phosphate 0.6kg, magnesium chloride 0.4kg, place proportion container to mix, and with being 6.8 with 1mol/L hydrochloric acid solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 115 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 35 ℃, add the lonely humicola lanuginosa solid fungicide of 3.0kg and 7.8kg brewer's yeast solid fungicide, and abundant mixing, under aerobic condition, 27 ℃ of fermentation 5d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 11
1. the preparation of useful fermenting agent: geotrichum candidum and candida utili are inoculated into respectively in the scale-up medium prescription 3, under 25～30 ℃, aerobic condition, geotrichum candidum was cultivated 2 days, candida utili was cultivated 1.5 days, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 10% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 77.8kg(contains over dry material 70kg), ammonium sulfate 4kg, potassium dihydrogen phosphate 0.1kg, magnesium chloride 0.4kg, water 52kg places proportion container to mix, and with being 6.5 with 1mol/L hydrochloric acid solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 115 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 4.0kg Geotrichum liquid and 6.5kg candida utili bacterium liquid, and abundant mixing, under aerobic condition, 25 ℃ of fermentation 3d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 12
1. the preparation of useful fermenting agent: penicillium funiculosum and fission yeast are inoculated into respectively in the expansion culture medium prescription 3, under 25～30 ℃, aerobic condition, penicillium funiculosum was cultivated 5 days, fission yeast was cultivated 3 days, culture grinds behind 40 ℃ of constant pressure and dries evenly, the living bacteria count of gained microbial inoculum is greater than 2.0 hundred million/g, and the miscellaneous bacteria rate is lower than 30%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, obtain water content and be 45% wet scum silica frost, pulverize for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: wet scum silica frost 80kg(contains over dry material 44kg), diammonium hydrogen phosphate 1.2kg, tripotassium phosphate 3.0kg, magnesium sulfate 0.3kg, 8kg water places proportion container to mix, and with being 6.5 with 1mol/L hydrochloric acid solution adjusting pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use water vapour in 115 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 2.0kg penicillium funiculosum solid fungicide and 2.5kg fission yeast solid fungicide, and abundant mixing, under aerobic condition, 25 ℃ of fermentation 4d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Embodiment 13
1. the preparation of useful fermenting agent: penicillium funiculosum and candida tropicalis are inoculated into respectively in the scale-up medium prescription 1, under 25～30 ℃, aerobic condition, penicillium funiculosum was cultivated 4 days, candida tropicalis was cultivated 1.5 days, the living bacteria count of gained microbial inoculum is greater than 1.0 hundred million/g, and the miscellaneous bacteria rate is lower than 20%.
2. cane sugar factory scum silica frost preliminary treatment: cane sugar factory mixed juice scum silica frost is dewatered by plate compression, and obtaining water content through constant pressure and dry again is 8% dry dross, pulverizes for subsequent use.
3. prepare burden: take by weighing mixed material by following weight: dry dross 55kg(contains over dry material 50.6kg), urea 1.2kg, ammonium dihydrogen phosphate (ADP) 0.8kg, magnesium chloride 3.0kg, water 35.7kg places proportion container to mix, and with being 6.0 with 1mol/L hydrochloric acid pH value, form fermentation materials.
4. sterilization: fermentation materials is moved into disinfection tank, use sugar refinery exhaust steam in 115 ℃ of sterilization 20min.
5. inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to 30 ℃, add 4.0kg penicillium funiculosum liquid and 5.5kg Candida tropicalis liquid, and abundant mixing, under aerobic condition, 26 ℃ of fermented and cultured 4d.
6. deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
Claims (11)
1. high protein microbiological feed, it is characterized in that, described high protein microbiological feed is to add useful fermenting agent fermentation to make in mixed material, described mixed material is comprised of the material of following weight part ratio: 30～70 parts of cane sugar factory scum silica frost (in the over dry material), 0.1～4 part of non protein nitrogen, 0.1～3 part of phosphate, 0.1～3 part of magnesium salts, 30～65 parts in water, regulating the pH value is 4.5～7.5, add the useful fermenting agent that 2～20% usefulness beneficial microbe bacterial classifications account for described mixed material gross weight are made, effective total viable count of described useful fermenting agent is not less than 100,000,000/g, through mixing, fermented and cultured, dry, pulverize, get the high protein microbiological feed.
2. described high protein microbiological feed according to claim 1 is characterized in that, comprises the composition of following percentage by weight: crude protein 〉=38%, total amino acid content 〉=20%, crude fat 7～15%, crude fibre 7～18%.
3. described high protein microbiological feed according to claim 1 and 2, it is characterized in that described beneficial microbe bacterial classification is that long handle wood is mould, any one or more than one strain combination in the aspergillus niger, lonely humicola lanuginosa, penicillium funiculosum, geotrichum candidum, candida utili, brewer's yeast, fission yeast, candida tropicalis.
4. described high protein microbiological feed according to claim 3, it is characterized in that described non protein nitrogen is any one or more than one combination in urea, ammonium chloride, ammonium sulfate, carbonic hydroammonium, ammonium dihydrogen phosphate (ADP), diammonium hydrogen phosphate, urea phosphate, sodium nitrate, the potassium nitrate.
5. described high protein microbiological feed according to claim 3, it is characterized in that described phosphate is any one or more than one combination in tripotassium phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, tertiary sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, diammonium hydrogen phosphate, ammonium hydrogen phosphate, the sodium pyrophosphate.
6. described high protein microbiological feed according to claim 3 is characterized in that, described magnesium salts is any one or the two kinds of combinations in magnesium sulfate, the magnesium chloride.
7. described high protein microbiological feed according to claim 3 is characterized in that, described cane sugar factory scum silica frost derives from the cane sugar factory mixed juice and processes the product that obtains through the come-up clarification process.
One kind arbitrary such as claim 1 to 7 as described in the preparation method of high protein microbiological feed, it is characterized in that, may further comprise the steps:
(1) preparation of useful fermenting agent: the beneficial microbe bacterial classification is enlarged cultivation, make useful fermenting agent;
(2) cane sugar factory scum silica frost preliminary treatment: with fresh cane sugar refinery dross dehydration, obtain water content and be 40%～65% wet scum silica frost, pulverize, for subsequent use; Or with fresh cane sugar refinery dross dehydration, drying, obtain water content and be lower than 20% dry dross, pulverize, for subsequent use;
(3) batching: take by weighing mixed material by following weight part ratio: cane sugar factory scum silica frost 30～70 parts of (in the over dry material), 0.1～4 part of non protein nitrogen, 0.1～5 part of phosphate, 0.1～3 part of magnesium salts, 30～65 parts in water; Place proportion container to mix mixed material, regulating pH value is 4.5～7.5, forms fermentation materials;
(4) sterilization: fermentation materials is moved into disinfection tank, use steam in 100～121 ℃ of sterilization 15～20min;
(5) inoculation, fermented and cultured: move into round when the fermentation materials after to be sterilized is cooled to below 40 ℃, add the useful fermenting agent of mixed material gross weight 2～20%, and abundant mixing, under aerobic condition, 20～35 ℃ of fermentation 2～6d;
(6) deactivation, drying, pulverizing: the gross product that will ferment after the fermentation ends carries out the thalline deactivation, dehydrates, pulverizes, and namely gets the high protein microbiological feed.
9. the preparation method of high protein microbiological feed according to claim 8, it is characterized in that described useful fermenting agent contains that long handle wood is mould, any one or more than one beneficial microbe strain combination in the aspergillus niger, lonely humicola lanuginosa, penicillium funiculosum, geotrichum candidum, candida utili, fission yeast, candida tropicalis.
10. the preparation method of high protein microbiological feed according to claim 9, it is characterized in that, described useful fermenting agent is liquid bacterial agent, it prepares in the following ways: with in the beneficial microbe bacterial classification any one or more than one be inoculated in the scale-up medium, under aerobic condition, 25～30 ℃ enlarge cultivation 1～5d, and the living bacteria count of gained microbial inoculum is greater than 100,000,000/g, and the miscellaneous bacteria rate is lower than 20%; Described scale-up medium prescription is comprised of the material of following weight part ratio: 16～20 parts in molasses, 75～85 parts in water, 0.3～0.8 part in urea, 0.1～0.3 part of potassium dihydrogen phosphate, regulating the pH value is 5.0～6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
11. the preparation method of high protein microbiological feed according to claim 9, it is characterized in that, described useful fermenting agent is solid fungicide, it prepares in the following ways: with in the beneficial microbe bacterial classification any one or more than one be inoculated in and enlarge in the culture medium, under aerobic condition, 25～30 ℃ enlarge cultivation 1～5d, culture in temperature≤50 ℃, constant pressure and dry or vacuum freeze-drying, grind or pulverize, mix, the living bacteria count of gained microbial inoculum is greater than 200,000,000/g, and the miscellaneous bacteria rate is lower than 30%; Described expansion culture medium prescription is comprised of the material of following weight part ratio: 45～50 parts in wheat bran, 1～3 part of sucrose, 45～55 parts in water, 0.1～0.5 part of diammonium hydrogen phosphate, regulating the pH value is 5.0～6.0,115 ℃ of water vapour sterilizations 20 minutes, and get final product.
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| CN109601740A (en) * | 2019-01-21 | 2019-04-12 | 广西大学 | A kind of bagasse fermentation live pig special microorganism feed and preparation method thereof |
| CN109601740B (en) * | 2019-01-21 | 2022-03-11 | 广西大学 | Bagasse fermented special microbial feed for live pigs and preparation method thereof |
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