CN102949506A - Traditional Chinese medicine extract for relieving itching and preventing allergy as well as preparation method, application and pharmaceutical composition of extract - Google Patents
Traditional Chinese medicine extract for relieving itching and preventing allergy as well as preparation method, application and pharmaceutical composition of extract Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine extract for relieving itching and preventing allergy as well as a preparation method, application and a medicine composition of the extract. The preparation method of the traditional Chinese medicine extract is carried out according to any one of the following manners: manner one: evenly mixing the raw materials and extracting by using water and/or a hydrophilic solvent; and manner two: individually extracting the ingredients of the raw materials by using the water and/or the hydrophilic solvent and then mixing evenly, wherein the raw material comprises the following components in parts by weight: 1-97 parts of fructus kochiae, 1-97 parts of fructus cnidii, 1-97 parts of pepper and 1-97 parts of radix sophorae flavescentis. The pharmaceutical composition of the traditional Chinese medicine extract has the obvious synergic allergy preventing and itching relieving effects, and has less side effects.
Description
Technical field
The invention belongs to natural medicine field, be specifically related to antipruritic antianaphylactic Chinese medicine extract and preparation method, application and pharmaceutical composition.
Background technology
Along with the development of society, people's living standard improve constantly, but incident is that increasing also from the social pressure of the each side such as work, life, various diseases is perplexing people, particularly the puzzlement of the diseases such as allergy, pruritus allows numerous people suffer untold misery especially.People are also increasing to the demand of safely and effectively natural antiallergic, antipruritic product.
One of dermopathic main Types of people is exactly, and from external source or endogenous material, stimulates mastocyte, and then produces histamine and act on teleneuron, thereby produces the symptoms such as erythema, pimple, pruritus, pain, allergy.In the prior art, in the majority with the chemosynthesis composition in antiallergic, the antipruritic product, side effect is larger, and antipruritic Claritin or the product of pure natural now, safety do not have substantially.
Summary of the invention
Technical problem to be solved by this invention be overcome in antiallergic of the prior art, the antipruritic product in the majority with synthetic ingredient, side effect is larger, and substantially there are not pure natural, the antipruritic Claritin of safety or the defective of product, a kind of have significantly collaborative antiallergic, antipruritic effect are provided, Chinese medicine extract that pure natural, safety, side effect are little and preparation method thereof, application and pharmaceutical composition.
The invention provides a kind of preparation method of Chinese medicine extract, undertaken by following either party's formula:
Mode one, after the even mixing of raw material, water and/or hydrophilic solvent extract and get final product;
Mode two after the independent water of each composition and/or hydrophilic solvent extraction with raw material, evenly mixes getting final product; Wherein, described raw material contains Fructus Kochiae 1-97 part of following portions by weight, Fructus Cnidii 1-97 part, and Pericarpium Zanthoxyli 1-97 part, Radix Sophorae Flavescentis 1-97 part, the gross weight umber is 100 parts.
Among the present invention, the described Fructus Kochiae, Fructus Cnidii, Pericarpium Zanthoxyli and Radix Sophorae Flavescentis those skilled in the art all know, are the Chinese crude drug that Chinese Pharmacopoeia is included the title definition.
Among the present invention, what described raw material was better is Fructus Kochiae 15-55 part of containing following portions by weight, Fructus Cnidii 15-55 part, and Pericarpium Zanthoxyli 15-55 part, Radix Sophorae Flavescentis 15-55 part, the gross weight umber is 100 parts; Better is 25 parts of Fructus Cnidiis, 25 parts in Pericarpium Zanthoxyli, and 25 parts of Radix Sophorae Flavescentiss, gross weight umber are 100 parts.
Among the present invention, described raw material is before using, and better can pulverize rear the use by this area routine operation, and the particle diameter of better pulverizing is 20～60 orders.
Among the present invention, described hydrophilic solvent is the common hydrophilic solvent in this area, one or more that better is in ethanol, methanol, glycerol, propylene glycol, butanediol and the dipropylene glycol.
Among the present invention, described raw material and water and/or hydrophilic solvent can be with magnitude relation that this area is conventional uses, that better is mass ratio 1:5～1:20, that better is mass ratio 1:8～1:15.When making water and hydrophilic solvent simultaneously, what the hydrophilic solvent consumption was better accounts for 50%～85% of water and hydrophilic solvent volume total amount for hydrophilic solvent.
Among the present invention, in mode one and the mode two, the condition of described extraction is this area normal condition, and what the extraction temperature was better is 50 ℃～100 ℃, and what the time of extraction was better is 2～4 hours, and what the number of times of extraction was better is 1 ~ 3 time.
Among the present invention, in mode one and the mode two, after the step of described extraction, it is better after relative density is 1.0～1.2 when also the extract of the water that obtains and/or hydrophilic solvent being concentrated into 20 ℃ of temperature, upper nonpolarity macroporous adsorptive resins chromatographic column, take ethanol water or the methanol aqueous solution eluting of volumetric concentration as 60%～95%, collect eluent and get final product afterwards.
Wherein, described nonpolar macroporous adsorption resin is the conventional said nonpolar macroporous adsorption resin in this area, and is better for HPD100 type nonpolar macroporous adsorption resin or D101 type nonpolar macroporous adsorption resin.
Wherein, the applied sample amount of the extract of described water and/or hydrophilic solvent is better is 1/10～10 times of nonpolar macroporous adsorption resin quality.
Wherein, the eluent consumption of described eluting is better is 1～4 times of quality of the extract of water and/or hydrophilic solvent.
That wherein, the flow velocity of described eluting is better is 2～4BV/h.
Among the present invention, also can the extracting solution that obtain be concentrated into water content less than mass percent 10% according to this area routine after the described Chinese medicine extract preparation.Wherein, described simmer down to this area routine operation, better is concentrating under reduced pressure.
The Chinese medicine extract that the present invention also provides the preparation method such as aforementioned Chinese medicine extract to make.
The present invention also provides a kind of pharmaceutical composition, and it contains aforesaid Chinese medicine extract and pharmaceutically acceptable excipient.
Among the present invention, described pharmaceutically acceptable carrier refers to the pharmaceutical carrier of pharmaceutical field routine, wherein, and diluent such as starch, Icing Sugar, dextrin, microcrystalline Cellulose, mannitol, lactose and Semen sojae atricolor wet goods; Binding agent such as polyvinylpyrrolidone or hydroxypropyl cellulose etc.; Disintegrating agent such as sodium carboxymethyl cellulose or low-substituted hydroxypropyl cellulose etc.; Lubricant such as magnesium stearate or Pulvis Talci etc.; Stabilizing agent such as sodium carboxymethyl cellulose or cyclodextrin etc.; Antiseptic such as ethylparaben or sodium benzoate etc.In addition, can also in this pharmaceutical composition, add other adjuvant such as flavouring agent and/or sweeting agent such as sucrose, fructose and aspartame etc.The active component of this pharmaceutical composition is the above-mentioned Rhizoma Acori Graminei extract of the present invention for the treatment of effective dose.This pharmaceutical composition can adopt the method for medical domain routine, and described active component and pharmaceutically acceptable carrier are made various dosage forms.When being used for it can being prepared into conventional solid preparation such as tablet, capsule, soft capsule, liquid preparation, granule, soft extract, pill, suspending agent, dispersant, syrup or suppository etc. when oral; When being used for injection, it can be prepared into injection.In various preparations, the content of Chinese medicine extract is mass percent 1% ~ 100%, and preferred content is mass percent 50% ~ 100%.
The present invention also provides aforesaid Chinese medicine extract at preparation antiallergic and/or antipruritic medicine, skin care item or washes the application of protecting in the product.
Among the present invention, described skin care item or wash that to protect product be that this area routine is said can be conventional aerosol, solution, suspensoid, Emulsion, ointment, semifluid preparation, tablet or powder.
Among the present invention, preparation skin care item or wash when protecting product, those skilled in the art can select essence or the antiseptic of any appropriate; As, antiseptic is generally selected parabens, benzoic acid/benzyl alcohol and derivatives class, imidazolidinyl urea or methyl isothiazole beautiful jade ketone and chloro thing thereof etc.; Essence is generally selected according to actual needs.
Agents useful for same of the present invention and raw material equal commercially available getting except specified otherwise.
On the basis that meets this area general knowledge, the optimum condition of each above-mentioned technical characterictic can combination in any obtain preferred embodiments of the present invention among the present invention.
Positive progressive effect of the present invention is:
Chinese medicine extract of the present invention suppresses the histamine releasing effect, and used drug level is starkly lower than the concentration of single Chinese medicine extract when using, and has the effect of higher inhibition histamine, and is safe and non-stimulating, can play antipruritic, antianaphylactic effect.The Chinese medicine extract that mode one of the present invention makes has more excellent antiallergic, antipruritic effect.
Description of drawings
Fig. 1 is that the various Chinese medicine extract of effect embodiment 1 are to the inhibitory action comparison diagram of the rat hypertrophy cell histamine releasing of antigen sensibilization.
The specific embodiment
Mode below by embodiment further specifies the present invention, but does not therefore limit the present invention among the described scope of embodiments.
The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Unless otherwise indicated, otherwise percentage ratio and umber calculate by weight; The value that relative density among each embodiment records when being 20 ℃.
Embodiment 1
Get Fructus Kochiae 25kg, Fructus Cnidii 25kg, Pericarpium Zanthoxyli 25kg, Radix Sophorae Flavescentis 25kg, solvent is extracted in preparation: volumetric concentration 50% ethanol water (V/V), configuration eluent: volumetric concentration 95% ethanol water (V/V), select non-polar resin post (HPD100 type, fast science equipment company limited rubs in Shanghai), former medicine: extract solvent quality ratio=1:20, extracting solution: resin: eluent mass ratio=10:1:10.
Fructus Kochiae powder is broken to 20～60 orders, extract solvent with 500L, under 75 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, through non-polar resin post absorb-elute, elution flow rate is 2BV/h, collection with the ethanol evaporate to dryness, is concentrated into 10～15kg(Fructus Kochiae extract by the eluent of resin column), do Quantitative Comparison for ease of detection of active, be diluted to identical multiple, add the 35kg propylene glycol, add water and add to the 50kg use, namely get Fructus Kochiae extracting solution (use separately, as a comparison case 1).
Fructus Cnidii is crushed to 20～60 orders, extract solvent with 500L, under 75 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, through non-polar resin post absorb-elute, elution flow rate is 2BV/h, collection with the ethanol evaporate to dryness, is concentrated into 10～15kg(Fructus cnidii extract by the eluent of resin column), do Quantitative Comparison for ease of detection of active, be diluted to identical multiple, add the 35kg propylene glycol, add water and add to the 50kg use, namely get Fructus Cnidii extracting solution (use separately, as a comparison case 2).
Zanthoxyli Bungeani powder is broken to 20～60 orders, extract solvent with 500L, under 75 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, through non-polar resin post absorb-elute, elution flow rate is 2BV/h, collection with the ethanol evaporate to dryness, is concentrated into 10～15kg(Fructus Zanthoxyli extract by the eluent of resin column), do Quantitative Comparison for ease of detection of active, be diluted to identical multiple, add the 35kg propylene glycol, add water and add to the 50kg use, namely get Pericarpium Zanthoxyli extracting solution (use separately, as a comparison case 3).
Radix Sophorae Flavescentis powder is broken to 20～60 orders, extracts solvent with 500L, under 75 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, and through non-polar resin post absorb-elute, elution flow rate is 2BV/h, collection with the ethanol evaporate to dryness, is concentrated into 10～15kg by the eluent of resin column, namely get Radix Sophorae Flavescentis extractive liquid, do Quantitative Comparison for ease of detection of active, be diluted to identical multiple, add the 35kg propylene glycol, adding water adds to 50kg and uses (use separately, as a comparison case 4).
Get above-mentioned Fructus Kochiae extracting solution 10kg, Fructus Cnidii extracting solution 10kg, Pericarpium Zanthoxyli extracting solution 10kg, Radix Sophorae Flavescentis extractive liquid 10kg, fully mixing namely gets Chinese medicine extract of the present invention.
Embodiment 2
Get Fructus Kochiae 15kg, Fructus Cnidii 15kg, Pericarpium Zanthoxyli 15kg, Radix Sophorae Flavescentis 55kg, solvent is extracted in preparation: volumetric concentration 50% ethanol water (V/V), configuration eluent: volumetric concentration 95% ethanol water (V/V), select non-polar resin post (D101 type, Tianjin Ourui Biology Technology Co., Ltd.), former medicine: extract the mass ratio=1:5 of solvent, extracting solution: resin: the mass ratio=1:10:4 of eluent.
The Fructus Kochiae, Fructus Cnidii, Pericarpium Zanthoxyli, Radix Sophorae Flavescentis powder are broken to 20～60 order mixings, extract solvent with 2000L, under 65 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, and through non-polar resin post absorb-elute, elution flow rate is 4BV/h, collection is by the eluent of resin column, with the ethanol evaporate to dryness, be concentrated into 40～60kg, do Quantitative Comparison for ease of detection of active, be diluted to identical multiple, add the 140kg propylene glycol, add water and add to 200kg, namely get Chinese medicine extract.
Embodiment 3
Get Fructus Kochiae 97kg, Fructus Cnidii 1kg, Pericarpium Zanthoxyli 1kg, Radix Sophorae Flavescentis 1kg; Configuration eluent: volumetric concentration 85% ethanol water (V/V).Select non-polar resin post (HPD100 type, Shanghai rub fast science equipment company limited), former medicine: extract solvent quality ratio=1:15, extracting solution: resin: the mass ratio=1:1:2 of eluent.
The Fructus Kochiae, Fructus Cnidii, Pericarpium Zanthoxyli, Radix Sophorae Flavescentis are crushed to respectively 20～60 orders, water is as extracting solvent, under 100 ℃ of conditions, extracted 3 hours respectively, being concentrated into relative density is between 1.0～1.2, and through non-polar resin post absorb-elute, elution flow rate is 3BV/h, collection is by the eluent of resin column, the eluting sections of 4 flavor medical materials are mixed, the ethanol evaporate to dryness to water content less than mass percent 10%, namely get Chinese medicine extract.
Embodiment 4
Get Fructus Kochiae 55kg, Fructus Cnidii 15kg, Pericarpium Zanthoxyli 15kg, Radix Sophorae Flavescentis 15kg, solvent is extracted in preparation: volumetric concentration 10% aqueous solution of propylene glycol (V/V), configuration eluent: volumetric concentration 80% ethanol water (V/V), select non-polar resin post (D101 type, Tianjin Ourui Biology Technology Co., Ltd.), former medicine: extract solvent quality ratio=1:8, extracting solution: resin: the mass ratio=5:1:20 of eluent.
The Fructus Kochiae, Fructus Cnidii, Pericarpium Zanthoxyli, Radix Sophorae Flavescentis powder are broken to 20～60 order mixings, extract solvent with 2000L, under 100 ℃ of conditions, extracted 3 hours, being concentrated into relative density is between 1.0～1.2, through non-polar resin post absorb-elute, elution flow rate is 2BV/h, collects the eluent by resin column, with the ethanol evaporate to dryness to water content less than mass percent 10%, namely get Chinese medicine extract.
Embodiment 5
Get Fructus Kochiae 20kg, Fructus Cnidii 20kg, Pericarpium Zanthoxyli 35kg, Radix Sophorae Flavescentis 25kg, solvent is extracted in preparation: volumetric concentration 50% ethanol water (V/V).
The Fructus Kochiae, Fructus Cnidii, Pericarpium Zanthoxyli, Radix Sophorae Flavescentis powder are broken to 20～60 order mixings, extract solvent with 2000L, under 65 ℃ of conditions, extracted 3 hours, filter, with ethanol evaporate to dryness in the filtrate, be concentrated into 40～60kg, add the 140kg propylene glycol, add water and add to 200kg, namely get Chinese medicine extract.
Embodiment 6
Get Fructus Kochiae 10kg, Fructus Cnidii 55kg, Pericarpium Zanthoxyli 10kg, Radix Sophorae Flavescentis 25kg, solvent is extracted in preparation: the aqueous solution (V/V) of volumetric concentration 25% methanol and 25% glycerol, extract temperature 50 degree, extract 3 times; All the other conditions are with embodiment 2.
Embodiment 7
Get Fructus Kochiae 10kg, Fructus Cnidii 10kg, Pericarpium Zanthoxyli 55kg, Radix Sophorae Flavescentis 25kg, solvent is extracted in preparation: volumetric concentration 50% butanediol solution (V/V), extract temperature 85 degree, extract 4 hours extraction times 1 time; All the other conditions are with embodiment 2.
Embodiment 8-10
The Chinese medicine extract consumption that embodiment 8-10 gets respectively embodiment 2-4 is mass percent 1%, 20% and 50%, with microcrystalline Cellulose, pregelatinized Starch and crospolyvinylpyrrolidone (these three kinds of composition equivalent, with Chinese medicine extract total amount to 100%) evenly mix, then mix with 5% alcoholic solution, granulation, drying, afterwards again with mix lubricant, tabletting gets final product, and namely gets the tablet of Chinese medicine extract.
Effect embodiment
1, equipment and material
Instrument: CO
2Incubator (SANYO GS); 405 type microplate reader (U.S. Bio-Rad company); Micro sample adding appliance (eppendorf); Centrifuge (eppendorf).
Reagent: testing sample is Comparative Examples 1-4 Fructus Kochiae extracting solution Fructus Cnidii extracting solution, Pericarpium Zanthoxyli extracting solution and the Radix Sophorae Flavescentis extractive liquid that obtains among embodiment 1, embodiment 2, the embodiment 1, be 10mg/ml and 1mg/ml with the PBS solution dilution, put in the refrigerator in 2 ℃ of-8 ℃ of preservations; Bovine serum albumin (BSA) (worker is given birth in Shanghai); Dimethyl benzene dialdehyde (OPT) (sigma); Compound 48/80(sigma).Tyrode culture fluid (1L): glucose 1g, NaCl 8g, KCl 0.2g, CaCl
22H
2O 0.2g, MgCl
20.1g, NaH
2PO
40.5g, HEPES 2.4g, 115 ℃ of 20min autoclavings.
Laboratory animal: the SD rat, male, body weight is 200 grams (buying in west, Shanghai pul-Bi Kai laboratory animal company limited) approximately.
2, experimental technique
2.1, the separation of rat peritoneal mast cells (Rat peritoneal mast cells, RPMC)
The disconnected cervical vertebra of normal rat is put to death, place 75% ethanol for disinfection sterilization (keeping its head to prevent that more than the ethanol liquid level ethanol from pouring into its abdomen), cut off the abdominal part fur, Tyrode culture fluid (the containing 0.1%BSA) 40ml of lumbar injection pre-cooling massages abdominal part gently with the rinsing abdominal cavity, draw rinsing liquid and place centrifugal (4 ℃ of sterilized centrifuge tube, 1500rpm, 10min), abandon supernatant, draw an amount of Tyrode culture fluid (containing 0.1%BSA) re-suspended cell, mastocyte concentration is about 10
5Cells/ml.
2.2, mast cell degranulation experiment
Divide the eppendorf that installs to after sterilization pipe with the mastocyte liquid behind the mixing, every pipe 200 μ l, the equal volume with 25 μ l in different samples adds different Chinese medicine extract, and mixing is inserted 5%CO immediately
237 ℃ of incubators are hatched 10min.Take out sample, add Compound 48/80(final concentration as 10 μ g/ml take 25 μ l equal volume), again insert 5%CO
2Take out after 37 ℃ of incubators are hatched 20min, sample is inserted ice chest cooling 10min with cessation reaction.Wherein, the Control sample does not add any Chinese herbal medicine extract and derivant, supply volume with 50 μ l Tyrode culture fluid (containing 0.1%BSA), the Compound 48/80 that the Model sample only adds 25 μ l to be inducing mast cell degranulation, and supplies residual volume with the Tyrode culture fluid (containing 0.1%BSA) of 25 μ l.
2.3, the mensuration of histamine
With the sample behind the reaction terminating centrifugal (4 ℃, 1500rpm, 10min), mastocyte can be deposited in EP pipe bottom in the sample after centrifugal, and supernatant is taken out, and inserts 4 ℃ of refrigerators stand-by.Mastocyte with the resuspended precipitation of Tyrode culture fluid (containing 0.1%BSA) of 250 μ l, after resuspended, insert 90 ℃ of water-bath heating, take out behind the 5min, insert immediately ice chest, thoroughly take out after the cooling until sample, again insert 90 ℃ of water-bath heating, repeated multiple times, utilize the principle of expanding with heat and contract with cold to reach the purpose of smudge cells.With the sample after the fragmentation centrifugal (4 ℃, 1500rpm, 10min), get supernatant.
Get each sample with after the 250 μ l distilled waters dilutions, add 125 μ l concentration and be the NaOH alkalization of 0.4mol/L, the concussion mixing also adds rapidly the methanol solution 100 μ l that mass fraction is 0.1% o-phthalaldehyde(OPA) (o-phthaldialdehyde, OPT), mixing immediately, ice bath reaction 40min.Adding such as 125 μ l concentration at each sample at last is that the HCl acidify of 0.5mol/L is to stop condensation reaction and to make fluorescent material stable.The sample of processing adds 96 orifice plates in the mode of each sample 3 hole, every hole 200 μ l, measures fluorescent value (excitation wavelength 350nm, emission wavelength 443nm).
3, result and discussion
The processing of data and analysis: each data is the average of 3 parallel multiple pipes.According to the fluorescence intensity that records, calculate the histamine releasing rate of each sample.And calculate Chinese medicine extract in each sample to the suppression ratio of mast cell degranulation reaction according to the histamine releasing rate.Computing formula is as follows:
Histamine releasing rate (Histamine Release Ratio)=supernatant histamine light absorption value/(supernatant histamine light absorption value+cell inner tissue amine light absorption value) * 100%
Suppression ratio (Inhibition Ratio)=[1-(sample histamine releasing rate-normal control tissue amine release rate)/(model control group is knitted amine release rate-normal control tissue amine release rate)] * 100%
By statistics, each sample Chinese medicine extract to the inhibitory action comparison diagram of the rat hypertrophy cell histamine releasing of antigen sensibilization as shown in Figure 1, concrete histamine releasing rate and histamine releasing suppression ratio result are recorded in table 1.
Table 1 Chinese medicine extract is to the inhibitory action of the rat hypertrophy cell histamine releasing of antigen sensibilization
| Sample | Histamine releasing rate (%) | Histamine releasing suppression ratio (%) |
| |
20 | / |
| Model | 79.3 | / |
| Blank | 77.4 | 3.2 |
| Embodiment 1 | 39.0 | 67.9 |
| Embodiment 2 | 26.2 | 89.5 |
| Comparative Examples 1, the Fructus Cnidii extracting solution | 54.3 | 42.1 |
| Comparative Examples 2, Radix Sophorae Flavescentis extractive liquid | 49.5 | 50.3 |
| Comparative Examples 3, Pericarpium Zanthoxyli extracting solution | 53.4 | 43.6 |
| Comparative Examples 4, Fructus Kochiae extracting solution | 51.8 | 46.3 |
Reach as shown in Figure 1 the experimental data of table 1 as can be known, Chinese medicine extract of the present invention obviously is better than single Chinese medicine extract to the inhibition of histamine releasing, illustrate that Chinese medicine extract of the present invention is suppressing to have synergism aspect the histamine releasing, safe and non-stimulating, can effectively play antipruritic, antianaphylactic effect.Further, the Chinese medicine extract of embodiments of the invention 2 is more excellent than the effect of the inhibition histamine releasing of embodiment 1.
The preparation of Application Example 1 antipruritic shampoo
By the good above-mentioned material of proportioning weighing, first A mutually in fatty alcohol ether sulfate sodium (TexaponN70, Cognis), disodiumedetate (EDTA-2Na, DOW) adds in the deionized water, 75 ℃ of lower stirrings, fully after the dissolving, add cocamido propyl betaine (CAB-35 is bestowed by heaven), be cooled to 45-50 ℃ under stirring; Add the B phase, add an amount of antiseptic and essence, pH value is controlled at 5.0-7.0, and the discharging that stirs obtains antipruritic shampoo.
The preparation of Application Example 2 Shu Min essences
By the good above-mentioned material of proportioning weighing, first A mutually in the Carbopol 941(U.S. reveal rich profit company) be dispersed in the deionized water, stir evenly, then the adding scattered hyaluronate sodium of butanediol after stirring, heats to 85-90 ℃, stirs; Be cooled to 45-50 ℃ under stirring; Add the B phase, add an amount of antiseptic and essence, pH value is controlled at 5.0-7.0, and the discharging that stirs obtains the Shu Min essence.
The preparation of Application Example 3 Shu Min facial creams
By the good above-mentioned material of proportioning weighing, first xanthan gum is dispersed in the glycerol, redispersion is in water and increase the temperature to simultaneously 85 ℃, then adds mutually other components of B, stirs; A in 85 ℃ of dissolve completes, is stirred; A is added to the mutually middle homogenizing that stirs of B, and approximately 4 minutes, froth breaking was cooled to 50 ℃; Add the C phase, add an amount of essence and antiseptic, pH value is controlled at 5.0-7.0, and the discharging that stirs obtains the Shu Min facial cream.
The preparation of Application Example 4 antipruritic waters
Embodiment 2: water=1:4, the good material of according to the above ratio weighing.Water is heated to first 85 ℃, kept 30 minutes, be cooled to 50 ℃; Add Chinese medicine extract, dissolve complete stirs, and adds an amount of essence and antiseptic, and pH value is controlled at 5.0-7.0, and the discharging that stirs obtains antipruritic water.
Claims (10)
1. the preparation method of a Chinese medicine extract is characterized in that, it is undertaken by following either party's formula:
Mode one, after the even mixing of raw material, water and/or hydrophilic solvent extract and get final product;
Mode two after the independent water of each composition and/or hydrophilic solvent extraction with raw material, evenly mixes getting final product;
Wherein, described raw material contains Fructus Kochiae 1-97 part of following portions by weight, Fructus Cnidii 1-97 part, and Pericarpium Zanthoxyli 1-97 part, Radix Sophorae Flavescentis 1-97 part, the gross weight umber is 100 parts.
2. the preparation method of Chinese medicine extract as claimed in claim 1 is characterized in that, described raw material is Fructus Kochiae 15-55 part of containing following portions by weight, Fructus Cnidii 15-55 part, and Pericarpium Zanthoxyli 15-55 part, Radix Sophorae Flavescentis 15-55 part, the gross weight umber is 100 parts; Better is 25 parts of Fructus Cnidiis, 25 parts in Pericarpium Zanthoxyli, and 25 parts of Radix Sophorae Flavescentiss, gross weight umber are 100 parts; Described raw material used after the better pulverizing before using; What the particle diameter of described pulverizing was better is 20～60 orders.
3. the preparation method of Chinese medicine extract as claimed in claim 1 is characterized in that, described hydrophilic solvent is one or more in ethanol, methanol, glycerol, propylene glycol, butanediol and the dipropylene glycol.
4. the preparation method of Chinese medicine extract as claimed in claim 1 is characterized in that, described raw material and water and/or hydrophilic solvent be mass ratio 1:5～1:20 with magnitude relation, that better is mass ratio 1:8～1:15; When making water and hydrophilic solvent simultaneously, what the hydrophilic solvent consumption was better accounts for 50%～85% of water and hydrophilic solvent volume total amount for hydrophilic solvent; What the condition of described extraction was better is: extracting temperature is 50 ℃～100 ℃, and the time of extraction is 2～4 hours, and the number of times of extraction is 1 ~ 3 time.
5. the preparation method of Chinese medicine extract as claimed in claim 1, it is characterized in that, after the step of described extraction, after relative density is 1.0～1.2 when also the extract of the water that obtains and/or hydrophilic solvent being concentrated into 20 ℃ of temperature, upper nonpolarity macroporous adsorptive resins chromatographic column, take ethanol water or the methanol aqueous solution eluting of volumetric concentration as 60%～95%, collect eluent and get final product afterwards.
6. the preparation method of Chinese medicine extract as claimed in claim 5 is characterized in that, described nonpolar macroporous adsorption resin is HPD100 type nonpolar macroporous adsorption resin or D101 type nonpolar macroporous adsorption resin; The applied sample amount of the extract of described water and/or hydrophilic solvent is 1/10～10 times of nonpolar macroporous adsorption resin quality; The eluent consumption of described eluting is 1～4 times of quality of the extract of water and/or hydrophilic solvent; The flow velocity of described eluting is 2～4BV/h.
7. such as the preparation method of claim 1 or 5 described Chinese medicine extract, it is characterized in that, also the extracting solution that obtains is concentrated into water content less than mass percent 10% after the described Chinese medicine extract preparation; Described concentrated better be concentrating under reduced pressure.
8. Chinese medicine extract that makes such as the preparation method of each described Chinese medicine extract of claim 1-7.
9. a pharmaceutical composition is characterized in that, it contains Chinese medicine extract claimed in claim 8 and pharmaceutically acceptable excipient; What the content of described Chinese medicine extract was better is mass percent 1% ~ 100%, and better is mass percent 50% ~ 100%.
10. Chinese medicine extract as claimed in claim 8 is at preparation antiallergic and/or antipruritic medicine, skin care item or wash the application of protecting in the product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101409685A CN102949506A (en) | 2012-05-08 | 2012-05-08 | Traditional Chinese medicine extract for relieving itching and preventing allergy as well as preparation method, application and pharmaceutical composition of extract |
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|---|---|---|---|
| CN2012101409685A CN102949506A (en) | 2012-05-08 | 2012-05-08 | Traditional Chinese medicine extract for relieving itching and preventing allergy as well as preparation method, application and pharmaceutical composition of extract |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735700A (en) * | 2014-01-17 | 2014-04-23 | 赵恒亮 | Medicinal bath preparation for treating sheep ectoparasite and preparation method thereof |
| CN104922266A (en) * | 2015-04-29 | 2015-09-23 | 杨卫国 | Antibacterial fluid for treating onychomycosis and tinea of feet and hands, and preparation method thereof |
| CN104958213A (en) * | 2015-06-26 | 2015-10-07 | 江苏奇力康皮肤药业有限公司 | Bathing shampoo capable of relieving itching and moistening and preparation method thereof |
| CN106880758A (en) * | 2017-03-10 | 2017-06-23 | 陈国忠 | It is a kind of for pruritus vulvue and the Chinese medicine preparation and preparation method of eczema stubborn dermatitis |
| CN111529463A (en) * | 2020-05-15 | 2020-08-14 | 广州比力福生物科技有限公司 | Compound plant anti-allergy agent |
| CN112891388A (en) * | 2021-02-10 | 2021-06-04 | 无限极（中国）有限公司 | Composite plant extract and preparation method and application thereof |
| CN113082129A (en) * | 2021-04-16 | 2021-07-09 | 黄冈师范学院 | Lotion for treating intractable skin itch and preparation method thereof |
| CN114224953A (en) * | 2022-01-06 | 2022-03-25 | 李成 | Itching relieving cream for treating anal itching and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735700A (en) * | 2014-01-17 | 2014-04-23 | 赵恒亮 | Medicinal bath preparation for treating sheep ectoparasite and preparation method thereof |
| CN103735700B (en) * | 2014-01-17 | 2016-04-27 | 赵恒亮 | The treatment verminal Medicatedbath lotion of sheep and compound method |
| CN104922266A (en) * | 2015-04-29 | 2015-09-23 | 杨卫国 | Antibacterial fluid for treating onychomycosis and tinea of feet and hands, and preparation method thereof |
| CN104958213A (en) * | 2015-06-26 | 2015-10-07 | 江苏奇力康皮肤药业有限公司 | Bathing shampoo capable of relieving itching and moistening and preparation method thereof |
| CN106880758A (en) * | 2017-03-10 | 2017-06-23 | 陈国忠 | It is a kind of for pruritus vulvue and the Chinese medicine preparation and preparation method of eczema stubborn dermatitis |
| CN111529463A (en) * | 2020-05-15 | 2020-08-14 | 广州比力福生物科技有限公司 | Compound plant anti-allergy agent |
| CN112891388A (en) * | 2021-02-10 | 2021-06-04 | 无限极（中国）有限公司 | Composite plant extract and preparation method and application thereof |
| CN113082129A (en) * | 2021-04-16 | 2021-07-09 | 黄冈师范学院 | Lotion for treating intractable skin itch and preparation method thereof |
| CN114224953A (en) * | 2022-01-06 | 2022-03-25 | 李成 | Itching relieving cream for treating anal itching and preparation method thereof |
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Application publication date: 20130306 |