CN102939100A - 用于消化鸡胚胎的酶组合物 - Google Patents

用于消化鸡胚胎的酶组合物 Download PDF

Info

Publication number
CN102939100A
CN102939100A CN2010800583217A CN201080058321A CN102939100A CN 102939100 A CN102939100 A CN 102939100A CN 2010800583217 A CN2010800583217 A CN 2010800583217A CN 201080058321 A CN201080058321 A CN 201080058321A CN 102939100 A CN102939100 A CN 102939100A
Authority
CN
China
Prior art keywords
embryo
virus
accutase
trypsin
dispase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2010800583217A
Other languages
English (en)
Chinese (zh)
Inventor
M·马里利亚诺
J·雷蒙德
M·圣-马里
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Publication of CN102939100A publication Critical patent/CN102939100A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN2010800583217A 2009-07-21 2010-07-21 用于消化鸡胚胎的酶组合物 Pending CN102939100A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP09305690 2009-07-21
EP09305690.1 2009-07-21
US26429909P 2009-11-25 2009-11-25
US61/264,299 2009-11-25
PCT/EP2010/004478 WO2011009613A1 (en) 2009-07-21 2010-07-21 Enzymatic composition for the digestion of chicken embryos

Publications (1)

Publication Number Publication Date
CN102939100A true CN102939100A (zh) 2013-02-20

Family

ID=43498784

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800583217A Pending CN102939100A (zh) 2009-07-21 2010-07-21 用于消化鸡胚胎的酶组合物

Country Status (13)

Country Link
US (1) US20120190100A1 (enExample)
EP (1) EP2456461A1 (enExample)
JP (1) JP2012533311A (enExample)
KR (1) KR20120089245A (enExample)
CN (1) CN102939100A (enExample)
AU (1) AU2010275764A1 (enExample)
CA (1) CA2768562A1 (enExample)
IN (1) IN2012DN01577A (enExample)
MX (1) MX2012000979A (enExample)
RU (1) RU2012106760A (enExample)
SG (1) SG178090A1 (enExample)
TW (1) TW201109440A (enExample)
WO (1) WO2011009613A1 (enExample)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105316309A (zh) * 2015-12-04 2016-02-10 广州赛莱拉干细胞科技股份有限公司 一种消化酶组合物及成纤维细胞和饲养层细胞的制备方法
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
WO2019129306A1 (zh) * 2017-12-26 2019-07-04 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
CN111117947A (zh) * 2020-01-09 2020-05-08 电子科技大学 一种鲤科鱼类肝细胞的快速分离方法
CN112458040A (zh) * 2020-12-23 2021-03-09 四川农业大学 一种成年牦牛瘤胃上皮细胞的分离培养方法
CN113136412A (zh) * 2021-04-22 2021-07-20 浙江珂瑞康生物医疗科技有限公司 用于含可溶性蛋白生物医用材料的生物负载测定处理液
CN113750224A (zh) * 2015-05-19 2021-12-07 科学蛋白实验室有限责任公司 在生产胰液素过程中减少或灭活病毒和微生物内容物的方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201508401TA (en) * 2010-10-11 2015-11-27 Abbvie Bahamas Ltd Processes for purification of proteins
EP2870173B1 (en) * 2012-07-09 2018-10-17 Roche Diagniostics GmbH Recombinantly produced neutral protease originating from paenibacillus polymyxa
CN104818238B (zh) * 2015-03-26 2019-12-20 四川农业大学 一种鸡肠上皮细胞分离培养方法
CN111936622B (zh) * 2018-01-19 2024-05-28 可隆生命科学株式会社 重组痘苗病毒和包含其的药物组合物
EP3787771A1 (en) * 2018-04-29 2021-03-10 REGENXBIO Inc. Scalable clarification process for recombinant aav production
CN117512055B (zh) * 2023-11-17 2024-08-23 广州元信生物科技有限公司 一种肝脏组织解离试剂盒及其解离方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692156A (zh) * 2002-09-05 2005-11-02 巴法里安诺迪克有限公司 在无血清条件下培养原代细胞和扩增病毒的方法
CN101490243A (zh) * 2006-07-12 2009-07-22 谢菲尔德大学 细胞生长培养基

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072565A (en) * 1974-11-04 1978-02-07 The Dow Chemical Company Production of viruses in tissue culture without use of serum
US4675187A (en) 1983-05-16 1987-06-23 Bristol-Myers Company BBM-1675, a new antibiotic complex
FR2643817B1 (fr) 1989-03-06 1993-12-17 Transgene Sa Composition pharmaceutique, utile a titre preventif ou curatif contre les tumeurs induites par les papillomavirus
GB8705477D0 (en) 1987-03-09 1987-04-15 Carlton Med Prod Drug delivery systems
GB9001641D0 (en) 1990-01-24 1990-03-21 Imp Cancer Res Tech Compounds
FR2668064B1 (fr) 1990-10-23 1994-12-16 Transgene Sa Composition pharmaceutique pour le traitement ou la prevention d'une tumeur maligne.
DE69434689D1 (de) 1993-06-10 2006-05-18 Genetic Therapy Inc Adenovirale vektoren für die behandlung der hämophilie
FR2710536B1 (fr) 1993-09-29 1995-12-22 Transgene Sa Usage anti-cancéreux d'un vecteur viral comportant un gène modulateur de la réponse immunitaire et/ou inflammatoire.
FR2751879B1 (fr) 1996-07-30 1998-10-30 Transgene Sa Composition pharmaceutique contre les tumeurs et infections a papillomavirus
FR2766091A1 (fr) 1997-07-18 1999-01-22 Transgene Sa Composition antitumorale a base de polypeptide immunogene de localisation cellulaire modifiee
FR2777570A1 (fr) 1998-04-17 1999-10-22 Transgene Sa Mutant ayant une activite phosphoribosyl transferase
FR2855758B1 (fr) 2003-06-05 2005-07-22 Biomerieux Sa Composition comprenant la polyproteine ns3/ns4 et le polypeptide ns5b du vhc, vecteurs d'expression incluant les sequences nucleiques correspondantes et leur utilisation en therapeutique
KR101062140B1 (ko) 2003-07-21 2011-09-05 트랜스진 에스.에이. 향상된 시토신 디아미네이즈 활성을 갖는 폴리펩티드
WO2005030940A1 (en) * 2003-09-29 2005-04-07 Seong Jun Yoon Highly bioactive and productive chicken embryo extract and method for preparation thereof
EP1791952A4 (en) * 2004-08-13 2008-06-11 Univ Georgia Res Found COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS
EP1807508A4 (en) * 2004-11-05 2008-03-12 Us Gov Health & Human Serv METHODS FOR PREPARING CELLS AND VIRUSES
US20100138947A1 (en) 2005-04-29 2010-06-03 Ivan Vassiliev Method for Producing Stem Cells or Stel Cell-Like Cells from Mammalian Embryos
EP2013229A2 (en) 2006-04-21 2009-01-14 Transgene S.A. Hpv-18-based papillomavirus vaccine
KR101082296B1 (ko) 2006-06-20 2011-11-09 트랜스진 에스.에이. 폭스바이러스 및 폭스바이러스 조성물 제조 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692156A (zh) * 2002-09-05 2005-11-02 巴法里安诺迪克有限公司 在无血清条件下培养原代细胞和扩增病毒的方法
CN101490243A (zh) * 2006-07-12 2009-07-22 谢菲尔德大学 细胞生长培养基

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OTTO-WILHELM MERTEN: "《Encyclopedia of Cell Technology》", 31 December 2003, article "Cell Detachment" *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113750224A (zh) * 2015-05-19 2021-12-07 科学蛋白实验室有限责任公司 在生产胰液素过程中减少或灭活病毒和微生物内容物的方法
CN113750224B (zh) * 2015-05-19 2024-04-30 科学蛋白实验室有限责任公司 在生产胰酶过程中减少或灭活病毒和微生物内容物的方法
US11952603B2 (en) 2015-05-19 2024-04-09 Scientific Protein Laboratories, Llc Method for reducing or inactivating viral and microbial content in the processes for the manufacture of pancreatin
CN105316309A (zh) * 2015-12-04 2016-02-10 广州赛莱拉干细胞科技股份有限公司 一种消化酶组合物及成纤维细胞和饲养层细胞的制备方法
WO2019129306A1 (zh) * 2017-12-26 2019-07-04 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
US11932869B2 (en) 2017-12-26 2024-03-19 Eyecure Therapeutics, Inc. (Jiangsu) Method for digestion of RPE cells
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
CN111117947B (zh) * 2020-01-09 2022-03-08 电子科技大学 一种鲤科鱼类肝细胞的快速分离方法
CN111117947A (zh) * 2020-01-09 2020-05-08 电子科技大学 一种鲤科鱼类肝细胞的快速分离方法
CN112458040B (zh) * 2020-12-23 2022-07-05 四川农业大学 一种成年牦牛瘤胃上皮细胞的分离培养方法
CN112458040A (zh) * 2020-12-23 2021-03-09 四川农业大学 一种成年牦牛瘤胃上皮细胞的分离培养方法
CN113136412A (zh) * 2021-04-22 2021-07-20 浙江珂瑞康生物医疗科技有限公司 用于含可溶性蛋白生物医用材料的生物负载测定处理液

Also Published As

Publication number Publication date
CA2768562A1 (en) 2011-01-27
WO2011009613A1 (en) 2011-01-27
US20120190100A1 (en) 2012-07-26
KR20120089245A (ko) 2012-08-09
RU2012106760A (ru) 2013-08-27
SG178090A1 (en) 2012-03-29
AU2010275764A1 (en) 2012-03-15
MX2012000979A (es) 2012-06-08
TW201109440A (en) 2011-03-16
AU2010275764A2 (en) 2012-03-15
JP2012533311A (ja) 2012-12-27
IN2012DN01577A (enExample) 2015-06-05
EP2456461A1 (en) 2012-05-30

Similar Documents

Publication Publication Date Title
CN102939100A (zh) 用于消化鸡胚胎的酶组合物
CN101460627B (zh) 制备痘病毒的方法以及痘病毒组合物
AU2010247371B2 (en) Method for orthopoxvirus production and purification
HK1174950A (en) Process for producing poxviruses and poxvirus compositions

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20130220