CN102934819A - Trepang capsule and preparation process thereof - Google Patents

Abstract

The invention relates to a trepang capsule and a preparation process thereof. The trepang capsule is composed of 500-1000 parts of trepang, 50-100 parts of rhodiola root, 50-80 parts of mulberry leaf, 50-80 parts of Chinese wolfberry; 50-80 parts of hawthorn, 50-80 parts of Chinese date, 10-20 parts of fructus momordicae, 50-80 parts of calcium gluconate, and 50-80 parts of vitamin C. The preparation process comprises the following eleven steps: raw material purification, vacuum freeze drying, coarse cracking, superfine grinding, microwave auxiliary enzymolysis, enzyme deactivation, filtration, desalting, auxiliary seasoning processing, material proportioning and pelletization, as well as sterilization and packing. The obtained trepang capsule is reasonable in formulation composition, and stable in property, has blood sugar decreasing and blood pressure reducing efficacy, and is especially prominent in blood fat reducing efficacy.

Description

A kind of beche-de-mer capsules and preparation technology thereof

Technical field

The present invention relates to a kind of beche-de-mer capsules and preparation technology thereof.

Background technology

Sea cucumber belongs to the Echinodermata Holothuroidea, and the whole world approximately has sea cucumber kind more than 1100 at present, and wherein 40 kinds of left and right can be edible; China approximately has sea cucumber more than 140 to plant, wherein have about 20 kinds can be edible, 10 kinds of sea cucumbers have higher commodity value.Before centuries, China people just think that sea cucumber is not only a kind of delicacies, but also are a kind of tonic nutriments.China Ming Dynasty thanks between year in Wanli year and starts system work " five assorted ancient sacrificial utensils " just relevant for the record of sea cucumber: " sea cucumber, beach, the east area of the Liao River, coextensive with eastern and southern Liaoning Province has it, its benefit warm in nature, sufficient enemy joins, therefore named sea cucumber ".

Sea cucumber is the animality health food of high nutrition, high protein, and its nutritional labeling is mainly sterol and Triterpenoids.Research in recent years tentative confirmation Stichopus Japonicus Acidic Mucopolysaccharide has antitumor and blood coagulation resisting function, and the contained holothurin of actinopyga agassizi is inhibited to the S180 mouse tumor, and sea cucumber also has the Antiarthritic effect in addition, and old man's arthralgia is had to curative effect.

The defect of prior art has: one, in the processing of traditional sea cucumber, sea cucumber is pulverized with enzymolysis process incomplete, thereby destroys the nutritional labeling in sea cucumber, reduces the health-care efficacy of sea cucumber.Two, sea cucumber preparation nutritional labeling is more single, and formulating is less, and mouthfeel and nutritive value are all lower.

Summary of the invention

The invention provides a kind of formula and preparation technology thereof of beche-de-mer capsules, as a kind of formula capsule, it had both retained the main nutrient composition in the sea cucumber, compatible with other functional health care foods again, formula is reasonable, process stabilizing, and health-care effect is outstanding, definite reducing blood lipid, blood sugar and blood pressure effect are arranged, and wherein effect for reducing blood fat is particularly outstanding.

Technical scheme is as follows:

Beche-de-mer capsules of the present invention, by sea cucumber, rhodiola root, mulberry leaf, matrimony vine, hawthorn, date, Momordica grosvenori, calcium gluconae and vitamin C, formed, sea cucumber 500-1000 part wherein, rhodiola root 50-100 part, mulberry leaf 50-80 part, matrimony vine 50-80 part, hawthorn 50-80 part, date 50-80 part, Momordica grosvenori 10-20 part, calcium gluconae 50-80 part, vitamin C 50-80 part.

The preparation technology of beche-de-mer capsules is:

1, purification of raw materials: by the fresh and alive sea cucumbers of acceptance(check), along belly from first to last complete section open, gill, impurity is cleaned up.

2, vacuum freeze drying: the sea cucumber cleaned up is carried out to freeze drying, and cryogenic temperature is-20～-25 ℃, and vacuum is 5-15Pa, snap frozen 2-3h, freeze-drying final temperature 60-70 ℃.

3, coarse crushing: the sea cucumber after freeze drying is put into to pulverizer, pulverize 10-30min, cross 60 mesh sieves, obtain thick sea cucumber powder.

4, ultramicro grinding: first the sea cucumber meal is carried out to ultramicro grinding by airslide disintegrating mill, time 2-3h, obtain ultra micro sea cucumber powder; Then ultra micro sea cucumber powder is carried out to the nanometer pulverizing by the high speed shear ultrafine crusher, grinding time is 3-5h.

5, microwave-assisted enzyme solution: add pure water, sea cucumber powder and water are dissolved with the 1:5-10 mass ratio, obtain sea cucumber milk, compound protease (the pepsin: bromelain=2:1) of accurate weighing sea cucumber weight 0.3-0.4%, put into the container cleaned up through pure water, after adding appropriate pure water that compound protease is dissolved, put into enzymatic vessel together with sea cucumber milk, standing 1-2min in the microwave environment that is 500-1000W in intensity.

6, the enzyme that goes out: after enzymolysis completes, by enzymolysis liquid rapidly degree of intensification to 90 ℃ of enzyme 10-30min that go out; After completing the deactivation of enzyme, enzymatic vessel is stopped to heating, enzymolysis liquid is cooled to rapidly to normal temperature; The enzymatic vessel purified rinse water, flushing liquor is put into enzymolysis liquid.

7, filter: the enzymolysis liquid after cooling is first used to the centrifugal 20min of generic centrifuge, filter, get filtrate, the centrifugal 20min of low temperature ultrahigh speed, filter filtrate for later use.

8, desalination: by filtrate by nanofiltration with except freshen; Residue and then mistake 100 mesh sieves, obtain sea cucumber extract.

9, processing auxiliary material baste:

Get rhodiola root, mechanical lapping, gained rhodiola root meal is crossed 60 mesh sieves; Carbon dioxide supercritical extraction method is extracted rhodiola active ingredient, and wherein extraction temperature is 40 ℃, and extracting pressure is 28MPa, and entrainer is ethanol, and volume fraction 78% extracts 160 minutes; 6000g * high speed centrifugation 15 minutes, separate solvent, and (40 ℃ 0.1MPa), obtain drying under reduced pressure.

Get the mulberry leaf sheet after first frost, 25 ℃ of water rinses, spread and dry, in 25 ℃, 0.1MPa environment, place 4 hours, 100 ℃ of steam beatings 5 minutes, room temperature is cooling, 150 ℃ of baking ovens toast 15 seconds, 4 ℃ cooling, pulverizes, and crosses 80 mesh sieves, obtain the mulberry leaf meal, meal is dropped into to fluid energy mill and carry out ultramicro grinding, product is crossed 300 mesh sieves, obtains mulberry leaf powder.

Get matrimony vine, first decoct 2 hours with 8 times of water gagings, then decoct 1.5 hours with 6 times of water gagings, merge decocting liquid twice, film evaporation method is flung to moisture, reduction vaporization dry (0.1MPa, 20 ℃), and reduction vaporization dry (0.1MPa, 20 ℃), obtain wolfberry fruit extract.

Get hawthorn, soak 60min, then adopt the decocting cooking method to extract, extract 3 times, add water and doubly measure and be respectively 14 times, 12 times and 12 times, extraction time is respectively 3.0h, 2.5h and 2.5h, merges filtrate three times, and film drying method drying, obtain haw thorn extract.

Get date, dry and pulverize, cross 60 mesh sieves, obtain jujube powder; Jujube powder is put into to extraction equipment for fat, adopt absolute ethyl alcohol refluxing extraction 3 times, each 2h; Jujube powder after degreasing is refluxed adds 20 times of water gagings and soaks, and after regulating pH8, immersion 80min, takes the Microwave Extraction method to extract jujube powder, and time 5min, obtain Fructus Jujubae extract.

Get Momordica grosvenori, dry and pulverize, cross 60 mesh sieves, obtain mangosteen powder; Add 30 times of water gagings, under 50 ℃ of conditions, ultrasonic wave extracts 3 times, each 5min, and operating frequency 53KHz, power 150W, merge filtrate three times, and film drying method drying, obtain Fructus Monordicae extract.。

By above-mentioned mixing of materials, and add calcium gluconae and vitamin C, the pure water of 10 times of quality soaks, and moves in jacketed pan, in ultrasonic environment, heats, and ultrasonic frequency is 20-30KHz, and boiling temperature is 95-100 ℃, and the time is 2-4 hour; Filter with the centrifuge of 100 order filter clothes, obtain the auxiliary material baste.

10, batching and granulation:

Sea cucumber feed liquid, auxiliary material baste that enzymolysis is filtered carry out proportioning in the ratio of 1:1, mix and again pass through centrifugal filtration, and drying, obtain dry extract.

Add microcrystalline cellulose in quality 1:1 ratio, fully mix, cross 40 mesh sieves, put the vacuum desiccator inner drying.

With 75% ethanol softwood processed, with 14 eye mesh screens, granulate, in 70 ℃ of oven dry, obtain the beche-de-mer capsules particle.

11, sterilizing and filling: the method that sterilizing adopts pasteurization to combine with microwave sterilization.

At first product is placed in to 95 ℃ of conditions, sterilizing 15s, shift out; Put it into again the microwave quick-reaction system and carry out microwave sterilization, power 850-1000W, time 8-15min.

In the filling process, the qualified capsule product of filling should be put into to constant heat storage at any time and preserve, after the complete end of lid is rolled in same batch materials filling, again carry out ethylene oxide sterilizing.

Compared to the prior art, the technology of the present invention effect comprises:

1, to choose sea cucumber be primary raw material in the present invention, and compatible with rhodiola root, mulberry leaf, matrimony vine, hawthorn, date, Momordica grosvenori, calcium gluconae and vitamin C, and the prescription ratio has been carried out to detailed screening, and formula forms rationally, stable in properties.

2, the present invention conducts in-depth research sea cucumber pulverizing and enzymolysis process, has significantly improved the bioavilability of sea cucumber, has fully retained the main nutrient composition of sea cucumber.

3, the present invention has carried out system research to the pulverizing extraction process of rhodiola root, mulberry leaf, matrimony vine, hawthorn, date and Momordica grosvenori, by technology such as ultramicro grinding, makes the stripping rapidly of its active ingredient, improves extraction efficiency.

4, the present invention conducts in-depth research the product sterilization process, make and can reach sterilization effect, but the retained product nutritional labeling is not destroyed again.

5, gained beche-de-mer capsules of the present invention, have definite reducing blood lipid, hypoglycemic and blood pressure reduction effect, and wherein blood fat reducing function is especially outstanding.

The specific embodiment

Below in conjunction with specific embodiment, technical solution of the present invention is described in detail.

Embodiment 1:

Formula forms:

500 parts of sea cucumbers, 50 parts of rhodiola roots, 50 parts, mulberry leaf, 50 parts of matrimony vines, 50 parts of hawthorn, 50 parts, date, 10 parts of Momordica grosvenoris, 50 parts of calcium gluconaes, 50 parts of vitamin Cs.

The preparation technology of beche-de-mer capsules is:

1, purification of raw materials: by the fresh and alive sea cucumbers of acceptance(check), along belly from first to last complete section open, gill, impurity is cleaned up.

2, vacuum freeze drying: the sea cucumber cleaned up is carried out to freeze drying, and cryogenic temperature is-20 ℃, and vacuum is 5Pa, snap frozen 2h, 60 ℃ of freeze-drying final temperatures.

3, coarse crushing: the sea cucumber after freeze drying is put into to pulverizer, pulverize 10min, cross 60 mesh sieves, obtain thick sea cucumber powder.

4, ultramicro grinding: first the sea cucumber meal is carried out to ultramicro grinding by airslide disintegrating mill, time 2h, obtain ultra micro sea cucumber powder; Then ultra micro sea cucumber powder is carried out to the nanometer pulverizing by the high speed shear ultrafine crusher, grinding time is 3h.

5, microwave-assisted enzyme solution: add pure water, sea cucumber powder and water are dissolved with the 1:5 mass ratio, obtain sea cucumber milk, compound protease (the pepsin: bromelain=2:1) of accurate weighing sea cucumber weight 0.3%, put into the container cleaned up through pure water, after adding appropriate pure water that compound protease is dissolved, put into enzymatic vessel together with sea cucumber milk, standing 1min in the microwave environment that is 500W in intensity.

6, the enzyme that goes out: after enzymolysis completes, by enzymolysis liquid rapidly degree of intensification to 90 ℃ of enzyme 10min that go out; After completing the deactivation of enzyme, enzymatic vessel is stopped to heating, enzymolysis liquid is cooled to rapidly to normal temperature; The enzymatic vessel purified rinse water, flushing liquor is put into enzymolysis liquid.

7, filter: the enzymolysis liquid after cooling is first used to the centrifugal 20min of generic centrifuge, filter, get filtrate, the centrifugal 20min of low temperature ultrahigh speed, filter filtrate for later use.

8, desalination: by filtrate by nanofiltration with except freshen; Residue and then mistake 100 mesh sieves, obtain sea cucumber extract.

9, processing auxiliary material baste:

Get rhodiola root, mechanical lapping, gained rhodiola root meal is crossed 60 mesh sieves; Carbon dioxide supercritical extraction method is extracted rhodiola active ingredient, and wherein extraction temperature is 40 ℃, and extracting pressure is 28MPa, and entrainer is ethanol, and volume fraction 78% extracts 160 minutes; 6000g * high speed centrifugation 15 minutes, separate solvent, and (40 ℃ 0.1MPa), obtain drying under reduced pressure.

Get the mulberry leaf sheet after first frost, 25 ℃ of water rinses, spread and dry, in 25 ℃, 0.1MPa environment, place 4 hours, 100 ℃ of steam beatings 5 minutes, room temperature is cooling, 150 ℃ of baking ovens toast 15 seconds, 4 ℃ cooling, pulverizes, and crosses 80 mesh sieves, obtain the mulberry leaf meal, meal is dropped into to fluid energy mill and carry out ultramicro grinding, product is crossed 300 mesh sieves, obtains mulberry leaf powder.

Get matrimony vine, first decoct 2 hours with 8 times of water gagings, then decoct 1.5 hours with 6 times of water gagings, merge decocting liquid twice, film evaporation method is flung to moisture, reduction vaporization dry (0.1MPa, 20 ℃), and reduction vaporization dry (0.1MPa, 20 ℃), obtain wolfberry fruit extract.

Get hawthorn, soak 60min, then adopt the decocting cooking method to extract, extract 3 times, add water and doubly measure and be respectively 14 times, 12 times and 12 times, extraction time is respectively 3.0h, 2.5h and 2.5h, merges filtrate three times, and film drying method drying, obtain haw thorn extract.

Get date, dry and pulverize, cross 60 mesh sieves, obtain jujube powder; Jujube powder is put into to extraction equipment for fat, adopt absolute ethyl alcohol refluxing extraction 3 times, each 2h; Jujube powder after degreasing is refluxed adds 20 times of water gagings and soaks, and after regulating pH8, immersion 80min, takes the Microwave Extraction method to extract jujube powder, and time 5min, obtain Fructus Jujubae extract.

Get Momordica grosvenori, dry and pulverize, cross 60 mesh sieves, obtain mangosteen powder; Add 30 times of water gagings, under 50 ℃ of conditions, ultrasonic wave extracts 3 times, each 5min, and operating frequency 53KHz, power 150W, merge filtrate three times, and film drying method drying, obtain Fructus Monordicae extract.。

By above-mentioned mixing of materials, and add calcium gluconae and vitamin C, the pure water of 10 times of quality soaks, and moves in jacketed pan, in ultrasonic environment, heats, and ultrasonic frequency is 20KHz, and boiling temperature is 95 ℃, and the time is 4 hours; Filter with the centrifuge of 100 order filter clothes, obtain the auxiliary material baste.

10, batching and granulation:

Sea cucumber feed liquid, auxiliary material baste that enzymolysis is filtered carry out proportioning in the ratio of 1:1, mix and again pass through centrifugal filtration, and drying, obtain dry extract.

Add microcrystalline cellulose in quality 1:1 ratio, fully mix, cross 40 mesh sieves, put the vacuum desiccator inner drying.

With 75% ethanol softwood processed, with 14 eye mesh screens, granulate, in 70 ℃ of oven dry, obtain the beche-de-mer capsules particle.

11, sterilizing and filling: the method that sterilizing adopts pasteurization to combine with microwave sterilization.

At first product is placed in to 95 ℃ of conditions, sterilizing 15s, shift out; Put it into again the microwave quick-reaction system and carry out microwave sterilization, power 850W, time 15min.

In the filling process, the qualified capsule product of filling should be put into to constant heat storage at any time and preserve, after the complete end of lid is rolled in same batch materials filling, again carry out ethylene oxide sterilizing.

Embodiment 2:

Formula forms:

1000 parts of sea cucumbers, 100 parts of rhodiola roots, 80 parts, mulberry leaf, 80 parts of matrimony vines, 80 parts of hawthorn, 80 parts, date, 20 parts of Momordica grosvenoris, 80 parts of calcium gluconaes, 80 parts of vitamin Cs.

The preparation technology of beche-de-mer capsules is:

1, purification of raw materials: by the fresh and alive sea cucumbers of acceptance(check), along belly from first to last complete section open, gill, impurity is cleaned up.

2, vacuum freeze drying: the sea cucumber cleaned up is carried out to freeze drying, and cryogenic temperature is-25 ℃, and vacuum is 15Pa, snap frozen 3h, 70 ℃ of freeze-drying final temperatures.

3, coarse crushing: the sea cucumber after freeze drying is put into to pulverizer, pulverize 30min, cross 60 mesh sieves, obtain thick sea cucumber powder.

4, ultramicro grinding: first the sea cucumber meal is carried out to ultramicro grinding by airslide disintegrating mill, time 3h, obtain ultra micro sea cucumber powder; Then ultra micro sea cucumber powder is carried out to the nanometer pulverizing by the high speed shear ultrafine crusher, grinding time is 5h.

5, microwave-assisted enzyme solution: add pure water, sea cucumber powder and water are dissolved with the 1:10 mass ratio, obtain sea cucumber milk, compound protease (the pepsin: bromelain=2:1) of accurate weighing sea cucumber weight 0.4%, put into the container cleaned up through pure water, after adding appropriate pure water that compound protease is dissolved, put into enzymatic vessel together with sea cucumber milk, standing 2min in the microwave environment that is 1000W in intensity.

6, the enzyme that goes out: after enzymolysis completes, by enzymolysis liquid rapidly degree of intensification to 90 ℃ of enzyme 30min that go out; After completing the deactivation of enzyme, enzymatic vessel is stopped to heating, enzymolysis liquid is cooled to rapidly to normal temperature; The enzymatic vessel purified rinse water, flushing liquor is put into enzymolysis liquid.

7, filter: the enzymolysis liquid after cooling is first used to the centrifugal 20min of generic centrifuge, filter, get filtrate, the centrifugal 20min of low temperature ultrahigh speed, filter filtrate for later use.

8, desalination: by filtrate by nanofiltration with except freshen; Residue and then mistake 100 mesh sieves, obtain sea cucumber extract.

9, processing auxiliary material baste:

Get rhodiola root, mechanical lapping, gained rhodiola root meal is crossed 60 mesh sieves; Carbon dioxide supercritical extraction method is extracted rhodiola active ingredient, and wherein extraction temperature is 40 ℃, and extracting pressure is 28MPa, and entrainer is ethanol, and volume fraction 78% extracts 160 minutes; 6000g * high speed centrifugation 15 minutes, separate solvent, and (40 ℃ 0.1MPa), obtain drying under reduced pressure.

Get the mulberry leaf sheet after first frost, 25 ℃ of water rinses, spread and dry, in 25 ℃, 0.1MPa environment, place 4 hours, 100 ℃ of steam beatings 5 minutes, room temperature is cooling, 150 ℃ of baking ovens toast 15 seconds, 4 ℃ cooling, pulverizes, and crosses 80 mesh sieves, obtain the mulberry leaf meal, meal is dropped into to fluid energy mill and carry out ultramicro grinding, product is crossed 300 mesh sieves, obtains mulberry leaf powder.

Get matrimony vine, first decoct 2 hours with 8 times of water gagings, then decoct 1.5 hours with 6 times of water gagings, merge decocting liquid twice, film evaporation method is flung to moisture, reduction vaporization dry (0.1MPa, 20 ℃), and reduction vaporization dry (0.1MPa, 20 ℃), obtain wolfberry fruit extract.

Get hawthorn, soak 60min, then adopt the decocting cooking method to extract, extract 3 times, add water and doubly measure and be respectively 14 times, 12 times and 12 times, extraction time is respectively 3.0h, 2.5h and 2.5h, merges filtrate three times, and film drying method drying, obtain haw thorn extract.

Get date, dry and pulverize, cross 60 mesh sieves, obtain jujube powder; Jujube powder is put into to extraction equipment for fat, adopt absolute ethyl alcohol refluxing extraction 3 times, each 2h; Jujube powder after degreasing is refluxed adds 20 times of water gagings and soaks, and after regulating pH8, immersion 80min, takes the Microwave Extraction method to extract jujube powder, and time 5min, obtain Fructus Jujubae extract.

Get Momordica grosvenori, dry and pulverize, cross 60 mesh sieves, obtain mangosteen powder; Add 30 times of water gagings, under 50 ℃ of conditions, ultrasonic wave extracts 3 times, each 5min, and operating frequency 53KHz, power 150W, merge filtrate three times, and film drying method drying, obtain Fructus Monordicae extract.。

By above-mentioned mixing of materials, and add calcium gluconae and vitamin C, the pure water of 10 times of quality soaks, and moves in jacketed pan, in ultrasonic environment, heats, and ultrasonic frequency is 30KHz, and boiling temperature is 100 ℃, and the time is 2 hours; Filter with the centrifuge of 100 order filter clothes, obtain the auxiliary material baste.

10, batching and granulation:

Sea cucumber feed liquid, auxiliary material baste that enzymolysis is filtered carry out proportioning in the ratio of 1:1, mix and again pass through centrifugal filtration, and drying, obtain dry extract.

Add microcrystalline cellulose in quality 1:1 ratio, fully mix, cross 40 mesh sieves, put the vacuum desiccator inner drying.

With 75% ethanol softwood processed, with 14 eye mesh screens, granulate, in 70 ℃ of oven dry, obtain the beche-de-mer capsules particle.

11, sterilizing and filling: the method that sterilizing adopts pasteurization to combine with microwave sterilization.

At first product is placed in to 95 ℃ of conditions, sterilizing 15s, shift out; Put it into again the microwave quick-reaction system and carry out microwave sterilization, power 1000W, time 8min.

In the filling process, the qualified capsule product of filling should be put into to constant heat storage at any time and preserve, after the complete end of lid is rolled in same batch materials filling, again carry out ethylene oxide sterilizing.

Claims (2)

1. a beche-de-mer capsules, it is characterized in that, beche-de-mer capsules consists of sea cucumber, rhodiola root, mulberry leaf, matrimony vine, hawthorn, date, Momordica grosvenori, calcium gluconae and vitamin C, wherein sea cucumber 500-1000 part, rhodiola root 50-100 part, mulberry leaf 50-80 part, matrimony vine 50-80 part, hawthorn 50-80 part, date 50-80 part, Momordica grosvenori 10-20 part, calcium gluconae 50-80 part, vitamin C 50-80 part.

2. the preparation technology of a kind of beche-de-mer capsules as claimed in claim 1, it is characterized in that, this preparation technology comprises purification of raw materials, vacuum freeze drying, coarse crushing, ultramicro grinding, microwave-assisted enzyme solution, the enzyme that goes out, filtration, desalination, processing auxiliary material baste, batching and granulation and sterilizing and filling totally ten one steps:

(1) purification of raw materials: by the fresh and alive sea cucumbers of acceptance(check), along belly from first to last complete section open, gill, impurity is cleaned up.

(2) vacuum freeze drying: the sea cucumber cleaned up is carried out to freeze drying, and cryogenic temperature is-20～-25 ℃, and vacuum is 5-15Pa, snap frozen 2-3h, freeze-drying final temperature 60-70 ℃.

(3) coarse crushing: the sea cucumber after freeze drying is put into to pulverizer, pulverize 10-30min, cross 60 mesh sieves, obtain thick sea cucumber powder.

(4) ultramicro grinding: first the sea cucumber meal is carried out to ultramicro grinding by airslide disintegrating mill, time 2-3h, obtain ultra micro sea cucumber powder; Then ultra micro sea cucumber powder is carried out to the nanometer pulverizing by the high speed shear ultrafine crusher, grinding time is 3-5h.

(5) microwave-assisted enzyme solution: add pure water, sea cucumber powder and water are dissolved with the 1:5-10 mass ratio, obtain sea cucumber milk, compound protease (the pepsin: bromelain=2:1) of accurate weighing sea cucumber weight 0.3-0.4%, put into the container cleaned up through pure water, after adding appropriate pure water that compound protease is dissolved, put into enzymatic vessel together with sea cucumber milk, standing 1-2min in the microwave environment that is 500-1000W in intensity.

(6) enzyme that goes out: after enzymolysis completes, by enzymolysis liquid rapidly degree of intensification to 90 ℃ of enzyme 10-30min that go out; After completing the deactivation of enzyme, enzymatic vessel is stopped to heating, enzymolysis liquid is cooled to rapidly to normal temperature; The enzymatic vessel purified rinse water, flushing liquor is put into enzymolysis liquid.

(7) filter: the enzymolysis liquid after cooling is first used to the centrifugal 20min of generic centrifuge, filter, get filtrate, the centrifugal 20min of low temperature ultrahigh speed, filter filtrate for later use.

(8) desalination: by filtrate by nanofiltration with except freshen; Residue and then mistake 100 mesh sieves, obtain sea cucumber extract.

(9) processing auxiliary material baste:

Get rhodiola root, mechanical lapping, gained rhodiola root meal is crossed 60 mesh sieves; Carbon dioxide supercritical extraction method is extracted rhodiola active ingredient, and wherein extraction temperature is 40 ℃, and extracting pressure is 28MPa, and entrainer is ethanol, and volume fraction 78% extracts 160 minutes; 6000g * high speed centrifugation 15 minutes, separate solvent, and (40 ℃ 0.1MPa), obtain drying under reduced pressure.

Get the mulberry leaf sheet after first frost, 25 ℃ of water rinses, spread and dry, in 25 ℃, 0.1MPa environment, place 4 hours, 100 ℃ of steam beatings 5 minutes, room temperature is cooling, 150 ℃ of baking ovens toast 15 seconds, 4 ℃ cooling, pulverizes, and crosses 80 mesh sieves, obtain the mulberry leaf meal, meal is dropped into to fluid energy mill and carry out ultramicro grinding, product is crossed 300 mesh sieves, obtains mulberry leaf powder.

Get matrimony vine, first decoct 2 hours with 8 times of water gagings, then decoct 1.5 hours with 6 times of water gagings, merge decocting liquid twice, film evaporation method is flung to moisture, reduction vaporization dry (0.1MPa, 20 ℃), and reduction vaporization dry (0.1MPa, 20 ℃), obtain wolfberry fruit extract.

Get hawthorn, soak 60min, then adopt the decocting cooking method to extract, extract 3 times, add water and doubly measure and be respectively 14 times, 12 times and 12 times, extraction time is respectively 3.0h, 2.5h and 2.5h, merges filtrate three times, and film drying method drying, obtain haw thorn extract.

Get date, dry and pulverize, cross 60 mesh sieves, obtain jujube powder; Jujube powder is put into to extraction equipment for fat, adopt absolute ethyl alcohol refluxing extraction 3 times, each 2h; Jujube powder after degreasing is refluxed adds 20 times of water gagings and soaks, and after regulating pH8, immersion 80min, takes the Microwave Extraction method to extract jujube powder, and time 5min, obtain Fructus Jujubae extract.

Get Momordica grosvenori, dry and pulverize, cross 60 mesh sieves, obtain mangosteen powder; Add 30 times of water gagings, under 50 ℃ of conditions, ultrasonic wave extracts 3 times, each 5min, and operating frequency 53KHz, power 150W, merge filtrate three times, and film drying method drying, obtain Fructus Monordicae extract.。

By above-mentioned mixing of materials, and add calcium gluconae and vitamin C, the pure water of 10 times of quality soaks, and moves in jacketed pan, in ultrasonic environment, heats, and ultrasonic frequency is 20-30KHz, and boiling temperature is 95-100 ℃, and the time is 2-4 hour; Filter with the centrifuge of 100 order filter clothes, obtain the auxiliary material baste.

(10) batching and granulation:

Sea cucumber feed liquid, auxiliary material baste that enzymolysis is filtered carry out proportioning in the ratio of 1:1, mix and again pass through centrifugal filtration, and drying, obtain dry extract.

Add microcrystalline cellulose in quality 1:1 ratio, fully mix, cross 40 mesh sieves, put the vacuum desiccator inner drying.

With 75% ethanol softwood processed, with 14 eye mesh screens, granulate, in 70 ℃ of oven dry, obtain the beche-de-mer capsules particle.

(11) sterilizing and filling: the method that sterilizing adopts pasteurization to combine with microwave sterilization.

At first product is placed in to 95 ℃ of conditions, sterilizing 15s, shift out; Put it into again the microwave quick-reaction system and carry out microwave sterilization, power 850-1000W, time 8-15min.

In the filling process, the qualified capsule product of filling should be put into to constant heat storage at any time and preserve, after the complete end of lid is rolled in same batch materials filling, again carry out ethylene oxide sterilizing.