CN102933074B - 由xy es细胞制备能育的xy雌性动物 - Google Patents

由xy es细胞制备能育的xy雌性动物 Download PDF

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Publication number
CN102933074B
CN102933074B CN201180028717.1A CN201180028717A CN102933074B CN 102933074 B CN102933074 B CN 102933074B CN 201180028717 A CN201180028717 A CN 201180028717A CN 102933074 B CN102933074 B CN 102933074B
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cells
mice
basal medium
female
donor
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Chinese (zh)
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CN102933074A (zh
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W·奥尔巴克
T·德希阿拉
W·普埃米洛
D·弗伦德维
D·巴伦苏埃拉
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Regeneron Pharmaceuticals Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0091—Oxidoreductases (1.) oxidizing metal ions (1.16)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201180028717.1A 2010-06-11 2011-06-10 由xy es细胞制备能育的xy雌性动物 Active CN102933074B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510101534.8A CN104711218B (zh) 2010-06-11 2011-06-10 由xy es细胞制备能育的xy雌性动物

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US35389610P 2010-06-11 2010-06-11
US61/353,896 2010-06-11
US13/157,728 US9149026B2 (en) 2010-06-11 2011-06-10 Production of fertile XY animals from XY ES cells
US13/157,728 2011-06-10
PCT/US2011/039997 WO2011156723A1 (en) 2010-06-11 2011-06-10 Production of fertile xy female animals from xy es cells

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CN201510101534.8A Division CN104711218B (zh) 2010-06-11 2011-06-10 由xy es细胞制备能育的xy雌性动物

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CN102933074B true CN102933074B (zh) 2015-04-08

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CN201510101534.8A Active CN104711218B (zh) 2010-06-11 2011-06-10 由xy es细胞制备能育的xy雌性动物

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US (4) US9149026B2 (https=)
EP (1) EP2579711B1 (https=)
JP (3) JP2013528062A (https=)
CN (2) CN102933074B (https=)
AU (1) AU2011265306C1 (https=)
CA (1) CA2800534C (https=)
CY (1) CY1121955T1 (https=)
DK (1) DK2579711T3 (https=)
ES (1) ES2727738T3 (https=)
MY (2) MY191870A (https=)
PL (1) PL2579711T3 (https=)
PT (1) PT2579711T (https=)
SG (1) SG185703A1 (https=)
WO (1) WO2011156723A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY191870A (en) 2010-06-11 2022-07-18 Regeneron Pharma Production of fertile xy female animals from xy es cells
SG11201406547YA (en) 2012-04-25 2014-11-27 Regeneron Pharma Nuclease-mediated targeting with large targeting vectors
DK3456831T3 (da) 2013-04-16 2021-09-06 Regeneron Pharma Målrettet modifikation af rottegenom
KR102170502B1 (ko) 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 게놈의 표적화된 변형을 위한 방법 및 조성물
HRP20200529T1 (hr) 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
SI3161128T1 (sl) 2014-06-26 2019-02-28 Regeneron Pharmaceuticals, Inc. Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe
BR112017007770A2 (pt) * 2014-10-15 2018-01-16 Regeneron Pharma cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
KR102531016B1 (ko) 2014-11-21 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물
ES2947714T3 (es) 2014-12-19 2023-08-17 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso
MX388475B (es) * 2015-09-17 2025-03-20 Regeneron Pharma Selección de células pluripotentes para producir ratones hembra xy fértiles.

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1217746A (zh) * 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
CN1423522A (zh) * 1999-12-20 2003-06-11 安东尼·C·F·佩里 从培养细胞产生克隆胚胎和成年动物的方法
EP1516924A1 (en) * 2003-09-17 2005-03-23 Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) Generation of human embryonic stem cells from triploid zygotes
CN1649489A (zh) * 2002-02-27 2005-08-03 长尾恭光 一种制备种系嵌合动物的方法
CN101506354A (zh) * 2006-08-09 2009-08-12 维涡里斯公司 用胚胎干细胞制备转基因禽类的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
GB0031744D0 (en) * 2000-12-28 2001-02-07 Pig Improvement Co Uk Ltd Methods
JP2004248505A (ja) * 2001-09-21 2004-09-09 Norio Nakatsuji 移植抗原の一部または全てを欠除したｅｓ細胞由来の未分化な体細胞融合細胞およびその製造
WO2003073843A2 (en) * 2002-03-05 2003-09-12 Artemis Pharmaceuticals Gmbh Inbred embryonic stem-cell derived mice
KR101178786B1 (ko) 2004-03-23 2012-09-07 다이이찌 산쿄 가부시키가이샤 다능성 줄기세포의 증식 방법
ES2463476T3 (es) 2004-10-19 2014-05-28 Regeneron Pharmaceuticals, Inc. Método para generar un ratón homocigótico para una modificación genética
JP4595896B2 (ja) 2006-07-14 2010-12-08 パナソニック電工株式会社 静電霧化装置
GB0623635D0 (en) * 2006-11-27 2007-01-03 Stem Cell Sciences Uk Ltd Pluripotent cell growth media
JP5843775B2 (ja) 2009-10-13 2016-01-13 ステムセル テクノロジーズ インコーポレーティッド 幹細胞を分化させるための重量オスモル濃度の操作法
MY191870A (en) 2010-06-11 2022-07-18 Regeneron Pharma Production of fertile xy female animals from xy es cells
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
SI3161128T1 (sl) 2014-06-26 2019-02-28 Regeneron Pharmaceuticals, Inc. Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe
BR112017007770A2 (pt) 2014-10-15 2018-01-16 Regeneron Pharma cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1217746A (zh) * 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
CN1423522A (zh) * 1999-12-20 2003-06-11 安东尼·C·F·佩里 从培养细胞产生克隆胚胎和成年动物的方法
CN1649489A (zh) * 2002-02-27 2005-08-03 长尾恭光 一种制备种系嵌合动物的方法
EP1516924A1 (en) * 2003-09-17 2005-03-23 Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) Generation of human embryonic stem cells from triploid zygotes
CN101506354A (zh) * 2006-08-09 2009-08-12 维涡里斯公司 用胚胎干细胞制备转基因禽类的方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Non-equivalence of cloned and clonal mice.;Jinsong Li,etc.;《Current Biology》;20050920(第18期);第756页第1-2栏 *
XY female mice resulting from a heritable mutation in the primary testis-determining gene,Tdy.;ROBIN LOVELL-BADGE and ELIZABETH ROBERTSON;《Development》;19901231;第109卷;635-646 *
从早期胚胎多能干细胞生成的嵌合鼠;宋震涛等;《遗传学报》;19931227;第20卷(第6期);499-503 *
具高效种系嵌合能力的C57BL/6J小鼠ES细胞系的建立;童英等;《遗传学报》;19991010;第26卷(第5期);468-473 *
小鼠ES细胞种系嵌合体的获得;陈伟胜等;《遗传学报》;19990410;第26卷(第2期);126-134 *

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JP6310969B2 (ja) 2018-04-11
PT2579711T (pt) 2019-06-11
ES2727738T3 (es) 2019-10-18
US9149026B2 (en) 2015-10-06
AU2011265306A1 (en) 2012-12-20
CA2800534A1 (en) 2011-12-15
JP6310968B2 (ja) 2018-04-11
EP2579711B1 (en) 2019-04-17
CN104711218A (zh) 2015-06-17
US20110307968A1 (en) 2011-12-15
US9885058B2 (en) 2018-02-06
US20170218399A1 (en) 2017-08-03
DK2579711T3 (da) 2019-06-17
EP2579711A1 (en) 2013-04-17
AU2011265306C1 (en) 2015-01-22
WO2011156723A1 (en) 2011-12-15
US9398762B2 (en) 2016-07-26
MY172702A (en) 2019-12-10
CA2800534C (en) 2019-01-22
US20160295841A1 (en) 2016-10-13
AU2011265306B2 (en) 2014-08-07
US9655351B2 (en) 2017-05-23
CY1121955T1 (el) 2020-10-14
CN102933074A (zh) 2013-02-13
HK1211621A1 (en) 2016-05-27
PL2579711T3 (pl) 2019-09-30
SG185703A1 (en) 2013-01-30
US20150067901A1 (en) 2015-03-05
JP2016185167A (ja) 2016-10-27
CN104711218B (zh) 2018-09-25
JP2016185168A (ja) 2016-10-27
MY191870A (en) 2022-07-18
JP2013528062A (ja) 2013-07-08

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