CN102919065A - Method for determining regeneration capacity of italian ryegrasses with different stubble heights - Google Patents
Method for determining regeneration capacity of italian ryegrasses with different stubble heights Download PDFInfo
- Publication number
- CN102919065A CN102919065A CN2012104633150A CN201210463315A CN102919065A CN 102919065 A CN102919065 A CN 102919065A CN 2012104633150 A CN2012104633150 A CN 2012104633150A CN 201210463315 A CN201210463315 A CN 201210463315A CN 102919065 A CN102919065 A CN 102919065A
- Authority
- CN
- China
- Prior art keywords
- annual ryegrass
- subgroup
- stubble
- group
- experimental group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a method for determining regeneration capacity of italian ryegrasses with different stubble heights. The method comprises the steps of: cultivating the italian ryegrasses with different stubble heights; calculating regeneration capacity indexes of the italian ryegrasses by the ratio of stubble biomass of the italian ryegrasses before being defoliated to the biomass of a new leaf after being defoliated, comparing the regeneration capacity indexes, and judging that the italian ryegrass with a large value has stronger regeneration capacity. According to the method, the regeneration capacity of the italian ryegrasses with different stubble heights can be effectively measured, and the method has important significance on research of regeneration of pasture. The method disclosed by the invention is simple in measurement method, easy to operate, convenient to widely apply, and strong in practicability.
Description
Technical field
The present invention relates to a kind of scientific research methods, specifically a kind of method of measuring the high Annual Ryegrass regeneration capacity power of different stubbles.
Background technology
Herbage searched for food by plant-eating animal or the people for cut cut after, usually show " reviviscence ", this is that herbage is kept its own existence, resists one of the most basic biological property of plant-eating animal injury, and it is produced keeping the grassland, the raising grass yield also has important meaning.The height of left different stubbles behind the herbage defoliation can produce important impact to the regeneration of herbage, the difference of the organic substance that the acrial part that the different high meetings of stubble causes is stored, thus can produce different impacts to the regeneration of herbage.Because the essential problem that herbage grows again is a process of utilizing energy and material to make newborn organic substance, yet, the herbage defoliation not only can its photosynthesis of severe inhibition, reduce the supply of photosynthetic product, even in the situation that defoliation is serious, can cause photosynthesis to interrupt fully, so, because the difference of the organic product amount of storing in the body that institute's high difference of toothing causes behind the defoliation might produce very important effect to its regeneration.
The difference of the interior organic product amount of storing of body that the high difference of eliminating institute's toothing causes could really reflect the power of the regeneration capacity of the high perennial ryegrasses of different stubbles to the impact of regeneration, but has not yet to see the report about this respect.Seek a kind of effective method, difference by getting rid of the organic product amount of storing in the body that the high difference of stubble causes is on the impact of regeneration, could measure in essence the power of the high different perennial ryegrass regeneration capacity of stubble, this regeneration to research herbage has great importance, yet yet there are no so far the report about the concrete measuring method of this respect.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, provide a kind of mensuration Annual Ryegrass leaf regeneration capacity strong and weak diverse ways, its measuring method is simple, can detect the difference of the high different perennial ryegrass regeneration capacity powers of stubble.
The present invention addresses the above problem the technical scheme that adopts to be:
A kind of method of measuring the high Annual Ryegrass regeneration capacity power of different stubbles may further comprise the steps:
Step 1: choose 2 consistent week Annual Ryegrass 72 strains in age of growth, implant in 12 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in mean temperature, every day, average light was according to being under 8 hours conditions, cultivate after 70 days, this 12 basin Annual Ryegrass is divided into 2 groups at random, every group of 6 basins, namely experimental group a and experimental group b are divided into a1 subgroup and a2 subgroup to experimental group a more at random, experimental group b is divided into b1 subgroup and b2 subgroup at random, every subgroup 3 basins; Cut off the Annual Ryegrass blade among experimental group a and the experimental group b, a group stays respectively different stubbles high with the b group;
Directly get test subgroup a1 and b1, get test subgroup a1 and b1 after perhaps the Annual Ryegrass that has cut off leaf among experimental group a and the b being continued to cultivate also leaf-cutting, cultivation and leaf shearing method are to cultivate under 20 ℃ of conditions of average daily temperature, cut blade once every 6 days, continuously leaf-cutting is N time, and a group and b group were cut afterwards respectively at every turn and stayed before identical batch high;
Test subgroup a1 after the leaf-cutting and the root of b1 are separated with stubble, obtain the Annual Ryegrass stubble, the Annual Ryegrass stubble put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass stubble oven dry thing, then survey every basin Annual Ryegrass stubble oven dry amount with electronic balance, obtain respectively testing stubble biomass Ra and the Rb of subgroup a1 and test subgroup b1, for subsequent use;
Step 2: will test that Annual Ryegrass is to cultivate under the 20-25 ℃ of condition in temperature among subgroup a2 and the b2, after 6 days, cut newborn Annual Ryegrass blade in the step 1 institute high position of toothing, obtain the Annual Ryegrass Newborn Leaves, the Annual Ryegrass Newborn Leaves put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass Newborn Leaves oven dry thing, then survey every basin Annual Ryegrass Newborn Leaves oven dry amount with electronic balance, obtain respectively testing subgroup a2 and b2 Newborn Leaves biomass La and Lb, for subsequent use;
Step 3: utilize Annual Ryegrass stubble biomass Ra, the Rb record, Newborn Leaves biomass La, Lb, according to formula RIa=
* 100% and RIb=
* 100% calculates Annual Ryegrass regeneration capacity index, compares the size of RIa and RIb, and value the greater just illustrates that its regeneration capacity is stronger.
Beneficial effect of the present invention is:
A, the present invention can effectively measure the power of the high Annual Ryegrass regeneration capacity of different stubbles, and the regeneration of studying herbage is had great importance;
B, measuring method of the present invention are simple, and easy operating is convenient to extensive use, and practical;
C, instrument required for the present invention are simple, with low cost, have improved economic benefit.
Embodiment
The present invention is further elaborated below in conjunction with embodiment.
A kind of method of measuring the high Annual Ryegrass regeneration capacity power of different stubbles may further comprise the steps:
Step 1: choose 2 consistent week Annual Ryegrass 72 strains in age of growth, implant in 12 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in mean temperature, every day, average light was according to being under 8 hours conditions, cultivate after 70 days, this 12 basin Annual Ryegrass is divided into 2 groups at random, every group of 6 basins, namely experimental group a and experimental group b are divided into a1 subgroup and a2 subgroup to experimental group a more at random, experimental group b is divided into b1 subgroup and b2 subgroup at random, every subgroup 3 basins; Cut off the Annual Ryegrass blade among experimental group a and the experimental group b, a group stays respectively different stubbles high with the b group;
Directly get test subgroup a1 and b1, get test subgroup a1 and b1 after perhaps the Annual Ryegrass that has cut off leaf among experimental group a and the b being continued to cultivate also leaf-cutting, cultivation and leaf shearing method are to cultivate under 20 ℃ of conditions of average daily temperature, cut blade once every 6 days, continuously leaf-cutting is N time, and a group and b group were cut afterwards respectively at every turn and stayed before identical batch high;
Test subgroup a1 after the leaf-cutting and the root of b1 are separated with stubble, obtain the Annual Ryegrass stubble, the Annual Ryegrass stubble put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass stubble oven dry thing, then survey every basin Annual Ryegrass stubble oven dry amount with electronic balance, obtain respectively testing stubble biomass Ra and the Rb of subgroup a1 and test subgroup b1, for subsequent use;
Step 2: will test that Annual Ryegrass is to cultivate under the 20-25 ℃ of condition in temperature among subgroup a2 and the b2, after 6 days, cut newborn Annual Ryegrass blade in the step 1 institute high position of toothing, obtain the Annual Ryegrass Newborn Leaves, the Annual Ryegrass Newborn Leaves put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass Newborn Leaves oven dry thing, then survey every basin Annual Ryegrass Newborn Leaves oven dry amount with electronic balance, obtain respectively testing subgroup a2 and b2 Newborn Leaves biomass La and Lb, for subsequent use;
Step 3: utilize Annual Ryegrass stubble biomass Ra, the Rb record, Newborn Leaves biomass La, Lb, according to formula RIa=
* 100% and RIb=
* 100% calculates Annual Ryegrass regeneration capacity index, compares the size of RIa and RIb, and value the greater just illustrates that its regeneration capacity is stronger.
Embodiment 1
A kind of method of measuring the high Annual Ryegrass leaf regeneration capacity power of different stubbles may further comprise the steps:
Step 1: choose 2 consistent week Annual Ryegrass 72 strains in age of growth, implant in 12 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in mean temperature, every day, average light was according to being under 8 hours conditions, cultivate after 70 days, this 12 basin Annual Ryegrass is divided into 2 groups at random, every group of 6 basins, namely experimental group a and experimental group b are divided into a1 subgroup and a2 subgroup to experimental group a more at random, experimental group b is divided into b1 subgroup and b2 subgroup at random, every subgroup 3 basins; Cut off the Annual Ryegrass blade among experimental group a and the experimental group b, a group and b group stay respectively the stubble of 1cm and 5cm high, then get test subgroup a1 and b1, root is separated with stubble, obtain the Annual Ryegrass stubble, the Annual Ryegrass stubble put into 75 ℃ drying baker, oven dry obtains every basin Annual Ryegrass stubble oven dry thing, then surveys every basin Annual Ryegrass stubble oven dry amount with electronic balance, obtain respectively testing stubble biomass Ra and the Rb of subgroup a1 and test subgroup b1, for subsequent use;
Step 2: will test that Annual Ryegrass is to cultivate under 20 ℃ of conditions in temperature among subgroup a2 and the b2, after 6 days, cut newborn Annual Ryegrass blade in the step 1 institute high position of toothing, obtain the Annual Ryegrass Newborn Leaves, the Annual Ryegrass Newborn Leaves sent in 75 ℃ the drying baker, oven dry, obtain every basin Annual Ryegrass Newborn Leaves oven dry thing, then survey every basin Annual Ryegrass Newborn Leaves oven dry amount with electronic balance, obtain respectively testing subgroup a2 and b2 Newborn Leaves biomass La and Lb, for subsequent use;
Step 3: utilize Annual Ryegrass stubble biomass Ra, the Rb record, Newborn Leaves biomass La, Lb, according to formula RIa=
* 100% and RIb=
* 100% calculates Annual Ryegrass regeneration capacity index, compares the size of RIa and RIb, and value the greater just illustrates that its regeneration capacity is stronger;
The result shows, RIa is that 81.30%, RIb is that 70.19%, RIa is higher by 15.82% than RIb, namely cultivate 70 days after, in the situation that 1 defoliation, leave the high Annual Ryegrass for 1cm of stubble and have stronger regeneration capacity than toothing is high for the Annual Ryegrass of 5cm.
Embodiment 2
A kind of method of measuring the high Annual Ryegrass leaf regeneration capacity power of different stubbles may further comprise the steps:
Step 1: choose 2 consistent week Annual Ryegrass 72 strains in age of growth, implant in 12 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in mean temperature, every day, average light was according to being under 8 hours conditions, cultivate after 70 days, this 12 basin Annual Ryegrass is divided into 2 groups at random, every group of 6 basins, namely experimental group a and experimental group b are divided into a1 subgroup and a2 subgroup to experimental group a more at random, experimental group b is divided into b1 subgroup and b2 subgroup at random, every subgroup 3 basins; Cut off the Annual Ryegrass blade among experimental group a and the experimental group b, a group and b group stay respectively the stubble of 1cm and 5cm high, then be to cultivate under 20 ℃ of conditions in temperature, cut leaf once every 6 days in the incubation, a group and b group institute toothing height are respectively 1cm and 5cm equally, cut continuously 2 times, this process is total to leaf-cutting 3 times, after the 3rd leaf-cutting, get immediately test subgroup a1 and b1, root is separated with stubble, obtain the Annual Ryegrass stubble, the Annual Ryegrass stubble is put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass stubble oven dry thing, then survey every basin Annual Ryegrass stubble oven dry amount with electronic balance, obtain respectively testing stubble biomass Ra and the Rb of subgroup a1 and test subgroup b1, for subsequent use;
Step 2: will test that Annual Ryegrass is to cultivate under 25 ℃ of conditions in temperature among subgroup a2 and the b2, after 6 days, cut newborn Annual Ryegrass blade in the step 1 institute high position of toothing, obtain the Annual Ryegrass Newborn Leaves, the Annual Ryegrass Newborn Leaves put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass Newborn Leaves oven dry thing, then survey every basin Annual Ryegrass Newborn Leaves oven dry amount with electronic balance, obtain respectively testing subgroup a2 and b2 Newborn Leaves biomass La and Lb, for subsequent use;
Step 3: utilize Annual Ryegrass stubble biomass Ra, the Rb record, Newborn Leaves biomass La, Lb, according to formula RIa=
* 100% and RIb=
* 100% calculates Annual Ryegrass regeneration capacity index, compares the size of RIa and RIb, and value the greater just illustrates that its regeneration capacity is stronger;
The result shows, RIa is that 20.59%, RIb is that 37.51%, RIb is higher by 82.18% than RIa, namely in the situation that 3 defoliations, leaves the high Annual Ryegrass for 5cm of stubble and has stronger regeneration capacity than toothing is high for the Annual Ryegrass of 1cm.
Claims (1)
1. a method of measuring the high Annual Ryegrass regeneration capacity power of different stubbles is characterized in that, may further comprise the steps:
Step 1: choose 2 consistent week Annual Ryegrass 72 strains in age of growth, implant in 12 identical flowerpots with every basin 6 strains, mixed-matrix is housed in the flowerpot, mixed-matrix is comprised of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, it is 25 ℃ in mean temperature, every day, average light was according to being under 8 hours conditions, cultivate after 70 days, this 12 basin Annual Ryegrass is divided into 2 groups at random, every group of 6 basins, namely experimental group a and experimental group b are divided into a1 subgroup and a2 subgroup to experimental group a more at random, experimental group b is divided into b1 subgroup and b2 subgroup at random, every subgroup 3 basins; Cut off the Annual Ryegrass blade among experimental group a and the experimental group b, a group stays respectively different stubbles high with the b group;
Directly get test subgroup a1 and b1, get test subgroup a1 and b1 after perhaps the Annual Ryegrass that has cut off leaf among experimental group a and the b being continued to cultivate also leaf-cutting, cultivation and leaf shearing method are to cultivate under 20 ℃ of conditions of average daily temperature, cut blade once every 6 days, continuously leaf-cutting is N time, and a group and b group were cut afterwards respectively at every turn and stayed before identical batch high;
Test subgroup a1 after the leaf-cutting and the root of b1 are separated with stubble, obtain the Annual Ryegrass stubble, the Annual Ryegrass stubble put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass stubble oven dry thing, then survey every basin Annual Ryegrass stubble oven dry amount with electronic balance, obtain respectively testing stubble biomass Ra and the Rb of subgroup a1 and test subgroup b1, for subsequent use;
Step 2: will test that Annual Ryegrass is to cultivate under the 20-25 ℃ of condition in temperature among subgroup a2 and the b2, after 6 days, cut newborn Annual Ryegrass blade in the step 1 institute high position of toothing, obtain the Annual Ryegrass Newborn Leaves, the Annual Ryegrass Newborn Leaves put into 75 ℃ drying baker, oven dry, obtain every basin Annual Ryegrass Newborn Leaves oven dry thing, then survey every basin Annual Ryegrass Newborn Leaves oven dry amount with electronic balance, obtain respectively testing subgroup a2 and b2 Newborn Leaves biomass La and Lb, for subsequent use;
Step 3: utilize Annual Ryegrass stubble biomass Ra, the Rb record, Newborn Leaves biomass La, Lb, according to formula RIa=
* 100% and RIb=
* 100% calculates Annual Ryegrass regeneration capacity index, compares the size of RIa and RIb, and value the greater just illustrates that its regeneration capacity is stronger.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210463315.0A CN102919065B (en) | 2012-11-17 | 2012-11-17 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210463315.0A CN102919065B (en) | 2012-11-17 | 2012-11-17 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102919065A true CN102919065A (en) | 2013-02-13 |
| CN102919065B CN102919065B (en) | 2014-03-12 |
Family
ID=47634116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210463315.0A Expired - Fee Related CN102919065B (en) | 2012-11-17 | 2012-11-17 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102919065B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520146A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum |
| CN102565313A (en) * | 2011-12-15 | 2012-07-11 | 河南科技大学 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
-
2012
- 2012-11-17 CN CN201210463315.0A patent/CN102919065B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520146A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum |
| CN102565313A (en) * | 2011-12-15 | 2012-07-11 | 河南科技大学 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
Non-Patent Citations (3)
| Title |
|---|
| 刘丹等: "断根与外源细胞分裂素诱导去叶黑麦草耐牧性的研究", 《中国农学通报》, vol. 28, no. 14, 20 May 2012 (2012-05-20) * |
| 王永军等: "留茬高度与刈割时期对墨西哥玉米再生性能的影响", 《中国农业科学》, vol. 38, no. 8, 31 August 2005 (2005-08-31) * |
| 石凤翎等: "刈割对缘毛雀麦3个品系再生的影响", 《草业科学》, vol. 26, no. 9, 15 September 2009 (2009-09-15) * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102919065B (en) | 2014-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pyter et al. | Effects of rhizome size, depth of planting and cold storage on Miscanthus x giganteus establishment in the Midwestern USA | |
| Beard et al. | Effect of temperature and cutting on the growth of creeping bentgrass (Agrostis palustris Huds.) roots 1 | |
| CN102612947A (en) | Method for improving growth performance of lawn plants by adopting rare earth lanthanum | |
| CN104026078A (en) | Reticulitermes-flavipes indoor intensive breeding method | |
| CN102550490B (en) | Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same | |
| CN102919065B (en) | Method for determining regeneration capacity of italian ryegrasses with different stubble heights | |
| CN102919066B (en) | Method for determining regeneration capacity of italian ryegrasses with different stubble heights by ribosylzeatin | |
| Ball et al. | Seasonal differences in the capacity of perennial ryegrass to respond to gibberellin explained | |
| Na et al. | Management of perennial warm-season bioenergy grasses. II. Seasonal differences in elephantgrass and energycane morphological characteristics affect responses to harvest frequency and timing | |
| CN102565313B (en) | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves | |
| Walker et al. | A quantitative study of water stress effect on sugarcane photosynthesis | |
| CN105724365A (en) | Black soldier fly egg refrigeration preservation method | |
| CN102520146B (en) | Method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum | |
| Sreekumar et al. | Fluorescence transients as a selection tool for marine microalgal consortia in a raceway pond reactor for biofuel production | |
| CN105994368B (en) | A kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow | |
| CN103416235A (en) | Facility crop low-temperature stress diagnostic method | |
| Wei et al. | Effects of temperature on the development and reproduction of sugarcane top borer Scirpophaga excerptalis (Lepidoptera: Pyralidae) | |
| Gomez-del-Campo | Effect of water supply on leaf area development, stomatal activity, transpiration, and dry matter production and distribution in young olive trees | |
| Sun et al. | Time budget and activity rhythm of wild Great Bustard in winter | |
| CN103642696B (en) | A kind of method of butterfly orchid tissue cultured seedling fast breeding and fungal elicitor | |
| CN108307908A (en) | A kind of eucalyptus citriodora oil efficient business method of woods | |
| Khan et al. | Rhizome viability and shoot vigour in relation to Miscanthus establishment. | |
| Yoon et al. | Paulownia: China's wonder tree | |
| Zheng et al. | Research on the relationship among the growth period environmental factors of tomato under the condition of mulched drip irrigation in greenhouse | |
| CN105353094A (en) | Quick detection method for resistant digitaria sanguinalis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140312 Termination date: 20141117 |
|
| EXPY | Termination of patent right or utility model |