CN102565313A - Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves - Google Patents
Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves Download PDFInfo
- Publication number
- CN102565313A CN102565313A CN2011104204589A CN201110420458A CN102565313A CN 102565313 A CN102565313 A CN 102565313A CN 2011104204589 A CN2011104204589 A CN 2011104204589A CN 201110420458 A CN201110420458 A CN 201110420458A CN 102565313 A CN102565313 A CN 102565313A
- Authority
- CN
- China
- Prior art keywords
- itanlian rye
- itanlian
- stubble
- regeneration
- rye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves after removing leaves. The method comprises the following steps of: taking 36-48 plants of lolium multiflorum which are consistent in growth and 3 years old, culturing for 7 weeks under the conditions that the temperature is 18-25 DEG C and the illumination time is 6-8 hours every day, respectively detecting the average carbon storage Cr in the root parts of the lolium multiflorum before removing the leaves, the average carbon storage Cs in stubbles of the lolium multiflorum before removing the leaves and the total average carbon storage Cn in regenerated leaf blades of the lolium multiflorum, and calculating the relative maximum amount RI of the stored carbohydrates capable of being utilized for regeneration of the lolium multiflorum leaves. According to the method for measuring the stored carbohydrates capable of being utilized for regeneration of the lolium multiflorum leaves, provided by the invention, the measuring method is simple, the relative maximum amount of the stored carbohydrates which can be utilized for regeneration of the leaf blades of the lolium multiflorum before removing the leaves can be detected, the process is simple, the operation is simple, and the production efficiency and the working benefits can be further improved.
Description
Technical field
The present invention relates to a kind of measuring method, a kind of specifically method of measuring Itanlian rye leaf regeneration storage carbohydrate.
Background technology
Herbage searched for food by plant-eating animal or the people for cut cut after; Usually show " reproducibility "; This is that herbage is kept its own existence, resists one of the most basic biological characteristics of plant-eating animal injury, and it is produced keeping the grassland, the raising grass yield also all has important meaning.The regeneration of herbage be one relate to many-sided, complicated problems comparatively, but numerous regeneration problems must be faced a basic fact, promptly behind the defoliation the required carbohydrates of regeneration come source problem.Because the long again essential problem of herbage is a process of utilizing energy and material to make newborn organic substance; Yet the herbage defoliation not only can its photosynthesis of severe inhibition, reduces the supply of photosynthate; Even under the defoliation serious situation, can cause light action to interrupt fully.So, the photosynthetic organic product of storing in the herbage defoliation precursor, promptly the parent carbohydrates might produce important effect to its regeneration.
But present numerous scholar is to the research of pasture utilization carbohydrates; Only limit to produce wholesome effect to regeneration, or the carbohydrates that utilizes isotope technology checking regeneration to utilize has part to come from the carbohydrates of being stored before the defoliation from clear and definite its in aspect of matter.The carbohydrates of making the preceding storage of herbage defoliation clear has on earth and how much participates directly in the regeneration leaf growth process, the regeneration of studying herbage is had great importance, yet also do not see the report about the concrete measuring method of this respect so far.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, the method for measuring Itanlian rye leaf regeneration storage carbohydrate is provided, its measuring method is simple, can detect the relative maximum of the carbohydrates of the preceding storage of its defoliation that the regeneration of rye blade of grass can utilize.
The present invention addresses the above problem the technical scheme that is adopted to be: measure the method for Itanlian rye leaf regeneration storage carbohydrate, its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 6-8 strains; Mixed-matrix is made up of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, is 17-25 ℃ in temperature, and illumination every day is under 6-8 hours conditions; Cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 1-5cm, then 6 basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble; Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, be under 20-25 ℃ of conditions in temperature with the Itanlian rye among the test group b; Carrying out lucifuge cultivates; Every at a distance from 6 days, on the high position of step 1 institute toothing, cut newborn Itanlian rye blade, when stopping growing, all Itanlian ryes in test group b end; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves; Obtain the average total carbon reserves of Itanlian rye regeneration blade at last, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate.
Beneficial effect
A, measuring method of the present invention are simple, and easy operating is convenient to widespread use, and practical.
B, the present invention detect the relative quantity that regeneration rye blade of grass utilizes storage carbohydrate through measuring carbon, and when detecting carbon content, required instrument is simple, and is with low cost, improved economic benefit.
Embodiment
Measure the method for Itanlian rye leaf regeneration storage carbohydrate, its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 6-8 strains; Mixed-matrix by ratio of weight and the number of copies is 17-25 ℃ by 3 parts of vermiculites and the preparation of 1 part of turfy soil in temperature, under 6-8 hours conditions of illumination every day; After cultivating for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 1-5cm, then 6 above-mentioned basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after separate with stubble again, obtain the Itanlian rye root and the stubble; Above-mentioned Itanlian rye root and stubble are sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root and stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, the Itanlian rye among the test group b is carried out lucifuge cultivate under 20-25 ℃ condition; Every at a distance from 6 days; On the high position of step 1 institute toothing, cut newborn Itanlian rye blade, end when all Itanlian ryes in test group b all stop growing; The above-mentioned Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect the biomass and the carbon content of oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves afterwards; Calculate the average total carbon reserves of Itanlian rye regeneration blade again, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate.
Embodiment 1
Measure the method for Itanlian rye leaf regeneration storage carbohydrate, its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 36 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 6 strains; Mixed-matrix is made up of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, is 17 ℃ in temperature, and illumination every day is under 6 hours conditions; Cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 1cm, then 6 basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble; Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, be under 20 ℃ of conditions in temperature, carry out lucifuge and cultivate the Itanlian rye among the test group b, every at a distance from 6 days, on the high position of step 1 institute toothing, cut new life's Itanlian rye blade, cut 3 blades after Itanlian rye promptly stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves; Obtain the average total carbon reserves of Itanlian rye regeneration blade at last, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate;
The result shows that RI is 20.35%, promptly at jointing in the time of preceding 20 days, leaves that stubble is high be the Itanlian rye of 1cm, and the relative maximum of the carbohydrates of storage is 20.35% in its body that leaf regeneration is utilized.
Embodiment 2
Measure the method for Itanlian rye leaf regeneration storage carbohydrate, its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 42 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 7 strains; Mixed-matrix is made up of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, is 21 ℃ in temperature, and illumination every day is under 7 hours conditions; Cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 3cm, then 6 basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble; Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, be under 23 ℃ of conditions in temperature, carry out lucifuge and cultivate the Itanlian rye among the test group b, every at a distance from 6 days, on the high position of step 1 institute toothing, cut new life's Itanlian rye blade, cut 3 blades after Itanlian rye promptly stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves; Obtain the average total carbon reserves of Itanlian rye regeneration blade at last, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate;
The result shows that RI is 23.53%, promptly at jointing in the time of preceding 20 days, leaves that stubble is high be the Itanlian rye of 3cm, and the amount of maximum relatively of the carbohydrates of storing in its body that leaf regeneration is utilized is 23.53%.
Embodiment 3
Measure the method for Itanlian rye leaf regeneration storage carbohydrate, its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 48 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 8 strains; Mixed-matrix is made up of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, is 25 ℃ in temperature, and illumination every day is under 8 hours conditions; Cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 5cm, then 6 basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble; Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, be under 25 ℃ of conditions in temperature, carry out lucifuge and cultivate the Itanlian rye among the test group b, every at a distance from 6 days, on the high position of step 1 institute toothing, cut new life's Itanlian rye blade, cut 3 blades after Itanlian rye stopped growth; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves; Obtain the average total carbon reserves of Itanlian rye regeneration blade at last, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate;
The result shows that RI is 25.23%, promptly before jointing about 20 days the time, leaves that stubble is high be the Itanlian rye of 5cm, and the carbohydrates of storing in its body that leaf regeneration the is utilized amount of maximum relatively is 25.23%;
In technique scheme, used mixed-matrix is lighter in carrying, is prone to separate with root through washing, and has good water-retaining property and gas permeability simultaneously, and it is nutritious, helps the growth of Itanlian rye root system;
In technique scheme; The carbohydrate species of storing in the Itanlian rye body is very many, and glucose, sucrose, levulan and starch or the like are arranged, and these carbohydrates all contain carbon; Through measuring carbon, can reflect the relative quantity of carbohydrates;
In the technique scheme, the purpose that adopts lucifuge to cultivate is utilized the carbohydrates of storing in its body in order to make the rye grass leaf regeneration, because photosynthesis stops fully after the lucifuge, its regrowth can only utilize the carbohydrates of storing in its body;
In the technique scheme,, thereby let Itanlian rye stop growing, the utilization of the limit degree of the carbohydrates that so just can realize storing before leaf regeneration is to its defoliation through the repeatedly defoliation under the lucifuge condition.The carbon of storing before the defoliation that is utilized through defoliation rear blade regeneration; The preceding ratio of storing carbon of the leaf that accounts for; Just can reflect that the storage carbohydrate that much ratios are arranged on earth directly is used to the leaf growth of regenerating, so just obtain the relative maximum of Itanlian rye leaf regeneration storage carbohydrate.
Claims (1)
1. measure the method for Itanlian rye leaf regeneration storage carbohydrate, it is characterized in that: its measuring method is:
Step 1, choose 3 consistent week Itanlian rye 36-48 strains in age of growth, implant in 6 identical flowerpots, mixed-matrix is housed in the flowerpot with every basin 6-8 strains; Mixed-matrix is made up of 3 parts of vermiculites and 1 part of turfy soil by ratio of weight and the number of copies, is 17-25 ℃ in temperature, and illumination every day is under 6-8 hours conditions; Cultivated for 7 weeks, cut off the blade of Itanlian rye, all Itanlian ryes all stay identical stubble high; Stubble is high to be 1-5cm, then 6 basin Itanlian ryes is divided into 2 groups at random, every group 3 basin; Be test group a and test group b, subsequent use;
Step 2, get the Itanlian rye among the test group a; The root water of Itanlian rye in every basin is washed out, after the root of Itanlian rye is separated with stubble, obtain Itanlian rye root and Itanlian rye stubble; Itanlian rye root and Itanlian rye stubble sent in 75 ℃ the drying baker; Oven dry obtains every basin Itanlian rye root oven dry thing and Itanlian rye stubble oven dry thing, detects biomass and carbon content in every basin Itanlian rye root and the Itanlian rye stubble oven dry thing; After calculate Itanlian rye root before the defoliation average carbon reserves and the preceding Itanlian rye stubble of defoliation in average carbon reserves, subsequent use;
Step 3, be under 20-25 ℃ of conditions in temperature with the Itanlian rye among the test group b; Carrying out lucifuge cultivates; Every at a distance from 6 days, on the high position of step 1 institute toothing, cut newborn Itanlian rye blade, when stopping growing, all Itanlian ryes in test group b end; The Itanlian rye blade of at every turn cutting sent in 75 ℃ the drying baker; Oven dry; Detect biomass and carbon content in the oven dry thing, obtain the carbon reserves of every basin Itanlian rye regeneration blade behind each defoliation, calculate every basin regeneration blade total carbon reserves; Obtain the average total carbon reserves of Itanlian rye regeneration blade at last, subsequent use;
Step 4, utilize the average carbon reserves C of Itanlian rye root before the defoliation
r, the average carbon reserves C before the defoliation in the Itanlian rye stubble
sAverage total carbon reserves C with Itanlian rye regeneration blade
n, according to formula RI=
Calculate the relative maximum RI of Itanlian rye leaf regeneration storage carbohydrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110420458 CN102565313B (en) | 2011-12-15 | 2011-12-15 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110420458 CN102565313B (en) | 2011-12-15 | 2011-12-15 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102565313A true CN102565313A (en) | 2012-07-11 |
| CN102565313B CN102565313B (en) | 2013-10-16 |
Family
ID=46411271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110420458 Expired - Fee Related CN102565313B (en) | 2011-12-15 | 2011-12-15 | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102565313B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919066A (en) * | 2012-11-17 | 2013-02-13 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights by ribosylzeatin |
| CN102919065A (en) * | 2012-11-17 | 2013-02-13 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001095702A1 (en) * | 2000-06-14 | 2001-12-20 | Molecular Plant Breeding Nominees Ltd | Modification of lignin biosynthesis |
| CN102227981A (en) * | 2011-04-27 | 2011-11-02 | 天津师范大学 | Method for inhibiting growth of lawn weeds in planting lawn |
-
2011
- 2011-12-15 CN CN 201110420458 patent/CN102565313B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001095702A1 (en) * | 2000-06-14 | 2001-12-20 | Molecular Plant Breeding Nominees Ltd | Modification of lignin biosynthesis |
| CN102227981A (en) * | 2011-04-27 | 2011-11-02 | 天津师范大学 | Method for inhibiting growth of lawn weeds in planting lawn |
Non-Patent Citations (6)
| Title |
|---|
| A. MORVAN-BERTRAND ET AL: "Partitioning of reserve and newly assimilated carbon in roots and leaf tissues of Lolium perenne during regrowth after defoliation: assessment by 13C steady-state labelling and carbohydrate analysis", 《PLANT, CELL & ENVIRONMENT》 * |
| C.Y.WARD: "Carbohydrate Food Reserves and Leaf Area in Regrowth of Orchardgrass", 《CROP SCIENCE》 * |
| D. J. DONAGHY: "The importance of water-soluble carbohydrate reserves on regrowth and root growth of Lolium perenne (L.)", 《GRASS AND FORAGE SCIENCE》 * |
| DONAGHY ET AL: "Priority for allocation of water-soluble carbohydrate reserves during regrowth of Lolium perenne", 《GRASS AND FORAGE SCIENCE》 * |
| 温方等: "影响牧草再生性的因素分析", 《草原与草坪》 * |
| 白永飞: "刈割对牧草贮藏碳水化合物含量变化的影响", 《内蒙古农牧学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919066A (en) * | 2012-11-17 | 2013-02-13 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights by ribosylzeatin |
| CN102919065A (en) * | 2012-11-17 | 2013-02-13 | 河南科技大学 | Method for determining regeneration capacity of italian ryegrasses with different stubble heights |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102565313B (en) | 2013-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Verwijst et al. | Development of sustainable willow short rotation forestry in northern Europe | |
| Cui et al. | Starch accumulation in duckweed for bioethanol production | |
| CN101491194A (en) | High-efficiency environmental-protection Tuckahoe cultivation method | |
| CN102301882A (en) | Agricultural cultivation method of paddy-straw-goose ecology | |
| CN105145098B (en) | A kind of method of Java brucea cutting propagation | |
| CN103518514A (en) | Cultivation method allowing water bamboos to be harvested continuously in summer and autumn | |
| CN102093105A (en) | Culturing substrate for industrially cultivating agaricus bisporus and preparation method thereof | |
| CN101663996A (en) | Method for high yield, prematurity and dwarfing cultivation of jatropha curcas | |
| CN102565313B (en) | Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves | |
| CN115720851A (en) | Sugarcane somatic embryo and induction method thereof | |
| CN104206173B (en) | A kind of method utilizing wild Hericium erinaceus cultivating in bag | |
| CN106259208B (en) | A kind of method that tobacco plant repeatedly breeds aphidius gifuensis | |
| CN102550490B (en) | Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same | |
| CN102860241A (en) | Fruit-tree planting technology for improving room temperature of device | |
| Dubrovskis et al. | Biogas production from fresh maize biomass. | |
| CN102520146B (en) | Method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum | |
| CN103688864A (en) | Lilium oriental hybrid tissue culture seedling strengthening method | |
| CN103749126B (en) | The sustainable cultivation method of a kind of root of three-nerved spicebush | |
| CN102804986A (en) | Interplanting method for sorghum and cowpea | |
| UA81055U (en) | Method for cultivating calanoid copepods arctodiaptomus salinus (daday) | |
| Li et al. | Effects of deficit irrigation and planting modes on leaves' water physiological characteristics and grain yield of winter wheat | |
| CN202714018U (en) | Ground heating warming seedbed | |
| WO2008029173A2 (en) | Cultivation of sweet sorghum for bioethanol production | |
| CN110249952A (en) | A kind of cultural method improving the Changsha cigarette district tobacco efficiency of light energy utilization | |
| CN106613897B (en) | A method of cultivating grazing Brassica napus using distant hybridization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131016 Termination date: 20141215 |
|
| EXPY | Termination of patent right or utility model |